Brazilian journal of veterinary medicine最新文献_第4页

Description of the parasitic fauna of a specimen of Didelphis albiventris at Rio Grande do Sul 描述南里奥格兰德州的白琵鹭标本寄生动物群

Brazilian journal of veterinary medicine Pub Date : 2024-04-02 DOI: 10.29374/2527-2179.bjvm000524

J. Lignon, D. M. Pinto, S. G. Monteiro, N. S. Martins, Julia Victória de Souza, Giulia Ribeiro Meireles, Tamires Silva Dos Santos, F. Pappen, F. Bruhn

{"title":"Description of the parasitic fauna of a specimen of Didelphis albiventris at Rio Grande do Sul","authors":"J. Lignon, D. M. Pinto, S. G. Monteiro, N. S. Martins, Julia Victória de Souza, Giulia Ribeiro Meireles, Tamires Silva Dos Santos, F. Pappen, F. Bruhn","doi":"10.29374/2527-2179.bjvm000524","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm000524","url":null,"abstract":"Abstract Didelphis albiventris is considered the most common marsupial in Rio Grande do Sul. With omnivorous and synanthropic habits, it can serve as a host to various parasites, playing an important role in maintaining their biological cycle. Despite being a widespread and abundant species, it has a relatively little-known parasitic fauna. Therefore, the aim of this study was to report the diversity of parasites in a fecal sample from D. albiventris in Rio Grande do Sul, Southern Brazil. Modified Centrifugal-flotation and Spontaneous sedimentation techniques were used, revealing a high taxonomic diversity of parasites. Eggs of Ancylostoma spp., Toxocara spp., and Anoplocephalidae were reported for the first time in the host in the southern region of the country, along with the first report of pseudoparasitism by Syphacia spp. and Monocystis spp. in this animal species. The presence of different parasites in the feces of D. albiventris is of utmost importance, primarily for public health, but also for understanding the biodiversity of parasites present in wildlife, which has been poorly studied until now. This allows the implementation of effective strategies for controlling, preventing and treating these diseases.","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"132 ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140752223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of sarolaner in the treatment of tungiasis in naturally infested dogs. 使用沙罗来纳治疗自然感染的狗的东革热。

Brazilian journal of veterinary medicine Pub Date : 2024-03-13 eCollection Date: 2024-01-01 DOI: 10.29374/2527-2179.bjvm000224

Rafaella Tortoriello, Natália Lôres Lopes, Bianca Bianco Paschoalino Linhares, Thais Ribeiro Correia, Julio Israel Fernandes

{"title":"Use of sarolaner in the treatment of tungiasis in naturally infested dogs.","authors":"Rafaella Tortoriello, Natália Lôres Lopes, Bianca Bianco Paschoalino Linhares, Thais Ribeiro Correia, Julio Israel Fernandes","doi":"10.29374/2527-2179.bjvm000224","DOIUrl":"10.29374/2527-2179.bjvm000224","url":null,"abstract":"Tungiasis is an endemic dermatological parasitic zoonosis in Latin America, caused by the sand flea Tunga spp. (Siphonaptera, Tungidae), which promotes intense discomfort, swelling, erythema, itching, pain, secondary bacterial infection, cellulitis and necrosis. Sarolaner has been used to control different ectoparasites, but there is no record of its use for the treatment of tungiasis in dogs. The objective of this study was to evaluate the effectiveness of sarolaner for the treatment dogs naturally infested by Tunga spp. kept in the same infested environment. Three of four animals were medicated with sarolaner orally with a single dose of 2 mg/kg, as recommended by the manufacturer, and one animal remained without medication. After 24 hours, the fleas from all four dogs were mechanically removed. The animals were reevaluated on days +15 and +30 to assess possible reinfestation. The medicated animals remained free of fleas, while the untreated animal had fleas on the days previously defined for reevaluation. We can thus conclude that the use of sarolaner is an effective choice for tungiasis treatment.","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"46 ","pages":"e000224"},"PeriodicalIF":0.0,"publicationDate":"2024-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10938855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140133404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Solid epithelioid peritoneal mesothelioma with pulmonary metastasis in feline. 实性上皮样腹膜间皮瘤伴有肺转移的猫科动物。

Brazilian journal of veterinary medicine Pub Date : 2024-02-28 eCollection Date: 2024-01-01 DOI: 10.29374/2527-2179.bjvm004523

Heloísa Cristina Teixeira de Carvalho, Lígia Fernandes Gundim, Felipe Martins Pastor, Gabriel Henrique Guimarães, Arlinda Flores Coleto, Matias Pablo Juan Szabó, Alessandra Aparecida Medeiros-Ronchi

{"title":"Solid epithelioid peritoneal mesothelioma with pulmonary metastasis in feline.","authors":"Heloísa Cristina Teixeira de Carvalho, Lígia Fernandes Gundim, Felipe Martins Pastor, Gabriel Henrique Guimarães, Arlinda Flores Coleto, Matias Pablo Juan Szabó, Alessandra Aparecida Medeiros-Ronchi","doi":"10.29374/2527-2179.bjvm004523","DOIUrl":"10.29374/2527-2179.bjvm004523","url":null,"abstract":"Mesothelioma is a rare malignant neoplasm that affects the mesothelial cells lining the thoracic and abdominal cavities, such as the pleura, peritoneum, and pericardium. It is most prevalent in dogs and cattle, but the causes of this disease in animals are uncertain. In felines, it mainly affects the pleura, with an unfavorable prognosis. This paper explores a rare case of metastatic peritoneal mesothelioma in a 2-year-old female mixed breed cat, emphasizing its uniqueness due to the feline's age. The patient, previously treated at a private clinic, presented moderate abdominal distension as the only clinical sign. Abdominal ultrasound and peritoneal fluid cytology led to the provisional diagnosis of mesothelioma/carcinomatosis. One day after exploratory laparotomy, the animal died and was subsequently sent for necropsy. During macroscopic analysis, nodules were observed in the peritoneum, diaphragm, omentum, stomach serosa, and large intestine, and the diagnosis of solid epithelioid peritoneal mesothelioma with lung metastasis was confirmed after microscopic analysis. The diagnosis of mesothelioma is challenging, and the importance of immunohistochemical panels with specific markers such as cytokeratin AE1/AE3 and calretinin is highlighted. Considering that mesothelioma is a pathology with a poor prognosis, it is essential to include this disease in the list of differential diagnoses within veterinary oncology.","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"46 ","pages":"e004523"},"PeriodicalIF":0.0,"publicationDate":"2024-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10901465/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139991986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental enrichment interaction for laboratory beagle dogs used in research. 用于研究的实验用小猎犬的环境强化互动。

Brazilian journal of veterinary medicine Pub Date : 2024-02-16 eCollection Date: 2024-01-01 DOI: 10.29374/2527-2179.bjvm006323

Anna Julia Bessa Fernandes, Fernanda da Silva Freitas Campos, Gabriella Santos Oliveira, Priscila Cardim Oliveira, Debora Azevedo Borges, Ivan de Alamar Pedrosa, Fabio Barbour Scott

{"title":"Environmental enrichment interaction for laboratory beagle dogs used in research.","authors":"Anna Julia Bessa Fernandes, Fernanda da Silva Freitas Campos, Gabriella Santos Oliveira, Priscila Cardim Oliveira, Debora Azevedo Borges, Ivan de Alamar Pedrosa, Fabio Barbour Scott","doi":"10.29374/2527-2179.bjvm006323","DOIUrl":"10.29374/2527-2179.bjvm006323","url":null,"abstract":"Experimental animal facilities can have a negative impact on the well-being of animals owing to confinement. To mitigate this, environmental enrichment (EE) is implemented confinement. The purpose of EE is to enhance the complexity of an animal's natural environment. The objective of this study was to identify the types of EE most enjoyed by dogs used in experimental research and housed in individual kennels. A total of six adult Beagle dogs, housed at the Laboratório de Quimioterapia Experimental em Parasitologia Veterinária (LQEPV) at the Universidade Federal Rural do Rio de Janeiro (UFRRJ) were included in the study. The EE tools used included Petball® toys, a grassy outdoor area, interaction with other dogs and with a team member, a \"pool\" made of plastic bottles, and dog's wet food ice cream. A team member assessed the usage of these tools every 5 min for a 30-min period, six times per day, one day per week, over the course of eight weeks. The study revealed that the grass area was the tool most commonly used for physical enrichment, accounting for 58% of the occurrences (p < 0.05). Social and food enrichment were enjoyed in second and third place, with 23% and 19% of occurrences, respectively. that the study findings suggest that dogs housed in individual kennels enjoy engaging in their natural behaviors.","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"46 ","pages":"e006323"},"PeriodicalIF":0.0,"publicationDate":"2024-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10880118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lippia sidoides essential oil at concentration of 0.25% provided improvements in microbiota and intestine integrity of Danio rerio. 浓度为 0.25% 的茜草精油可改善丹利欧的微生物群和肠道完整性。

Brazilian journal of veterinary medicine Pub Date : 2024-02-14 eCollection Date: 2024-01-01 DOI: 10.29374/2527-2179.bjvm005323

Lucas Cardoso, Marco Shizuo Owatari, Francisco Célio Maia Chaves, Tamiris Henrique Ferreira, Domickson Silva Costa, William Eduardo Furtado, Marília Tedesco, Luciana Aparecida Honorato, José Luiz Pedreira Mouriño, Maurício Laterça Martins

{"title":"Lippia sidoides essential oil at concentration of 0.25% provided improvements in microbiota and intestine integrity of Danio rerio.","authors":"Lucas Cardoso, Marco Shizuo Owatari, Francisco Célio Maia Chaves, Tamiris Henrique Ferreira, Domickson Silva Costa, William Eduardo Furtado, Marília Tedesco, Luciana Aparecida Honorato, José Luiz Pedreira Mouriño, Maurício Laterça Martins","doi":"10.29374/2527-2179.bjvm005323","DOIUrl":"10.29374/2527-2179.bjvm005323","url":null,"abstract":"The study evaluated the effects of dietary supplementation with Lippia sidoides essential oil on the microbiota and intestinal morphology of Danio rerio. For this, 448 fish were randomly distributed in 28 tanks divided into a control group fed a commercial diet without supplementation, a group fed a commercial diet containing grain alcohol and five groups fed a commercial diet containing essential oil of L. sidoides (LSEO) at concentrations of 0.25%, 0.50%, 0.75%, 1.00% and 1.25%. After the period of dietary supplementation, biological materials were collected for microbiological and histological analyses. There were no significant differences regarding the microbiological count between the groups. Diversity of the microbiome was higher in 0.25% group than in control group. LSEO inhibited the growth of potentially pathogenic bacteria. Fish fed LSEO0.25% showed greater intestinal histomorphometric indices. The inclusion of LSEO at 0.25% in the diet of D. rerio provided improvements in fish microbiota and intestine integrity.","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"46 ","pages":"e005323"},"PeriodicalIF":0.0,"publicationDate":"2024-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10868529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139742838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An evaluation of techniques to diagnose Dioctophyme renale in dogs. 狗肾脏道真菌诊断技术评估。

Brazilian journal of veterinary medicine Pub Date : 2024-02-05 eCollection Date: 2024-01-01 DOI: 10.29374/2527-2179.bjvm006423

Gabriela de Almeida Capella, Josaine Cristina da Silva Rappeti, Natalia Berne Pinheiro, Soliane Carra Perera, Micaele Quintana de Moura, Marlete Brum Cleff, Caroline Maciel da Costa, Adriane Leites Strothmann, Guilherme Borges Weege, Carolina Silveira Mascarenhas, Maria Elisabeth Aires Berne

{"title":"An evaluation of techniques to diagnose Dioctophyme renale in dogs.","authors":"Gabriela de Almeida Capella, Josaine Cristina da Silva Rappeti, Natalia Berne Pinheiro, Soliane Carra Perera, Micaele Quintana de Moura, Marlete Brum Cleff, Caroline Maciel da Costa, Adriane Leites Strothmann, Guilherme Borges Weege, Carolina Silveira Mascarenhas, Maria Elisabeth Aires Berne","doi":"10.29374/2527-2179.bjvm006423","DOIUrl":"10.29374/2527-2179.bjvm006423","url":null,"abstract":"Dioctophyme renale is a nematode with zoonotic potential that affects the kidneys of carnivorous, wild, and domestic mammals. In this study, we sought to evaluate the indirect ELISA method against routine methods used to diagnose dioctophimosis. Hence, 38 dogs parasitized by D. renale, as confirmed by surgery, were selected. The dogs were evaluated by abdominal ultrasound and urinalysis, and their sera were tested by indirect ELISA using D. renale adult secretion and excretion antigen (DES). Five dogs were followed up with serum collections on day 0 (day of surgery) and 30, 60, and 90 days after surgery to evaluate antibody kinetics. Abdominal ultrasound and indirect ELISA successfully diagnosed 37 dogs parasitized by D. renale, while urinalysis diagnosed 29 animals. The positive animals were parasitized with 1-7 parasites; 17 dogs were infected by male and female parasites, 15 only by female parasites, and six were parasitized only by male parasites. When assessing specificity and sensitivity, all techniques showed 100% specificity and 81.6%, 97.4%, and 97.4% sensitivity for urinalysis, ultrasound, and ELISA, respectively (p < 0.001). The five positive dogs that were followed up after surgery showed a progressive decrease in mean absorbances in indirect ELISA (0.644, 0.516, 0.511, and 0.440, respectively). This study demonstrated that the indirect ELISA using the DE antigen could diagnose dioctophimosis regardless of the number, sex, and location of the parasites, with the potential to be used in epidemiological research and implementing immunological and molecular studies, opening new lines of research on D. renale.","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"46 ","pages":"e006423"},"PeriodicalIF":0.0,"publicationDate":"2024-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10851783/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139708706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bovine herpesvirus meningoencephalitis in the State of Tocantins, Brazil. 巴西托坎廷斯州的牛疱疹病毒脑膜脑炎。

Brazilian journal of veterinary medicine Pub Date : 2024-01-30 eCollection Date: 2024-01-01 DOI: 10.29374/2527-2179.bjvm004023

Ilgner Aimar Bezerra Pinheiro, Bianca Pereira Dias, Jardel Martins Ferreira, Alessandro José Ferreira Dos Santos, Sandro Estevan Moron, Gilzelle Maria da Luz Silva, Liana Bezerra Dias de Lima, Fabiano Mendes de Cordova

{"title":"Bovine herpesvirus meningoencephalitis in the State of Tocantins, Brazil.","authors":"Ilgner Aimar Bezerra Pinheiro, Bianca Pereira Dias, Jardel Martins Ferreira, Alessandro José Ferreira Dos Santos, Sandro Estevan Moron, Gilzelle Maria da Luz Silva, Liana Bezerra Dias de Lima, Fabiano Mendes de Cordova","doi":"10.29374/2527-2179.bjvm004023","DOIUrl":"10.29374/2527-2179.bjvm004023","url":null,"abstract":"Three outbreaks of herpesvirus meningoencephalitis in cattle have been reported in three municipalities in the northern region of the State of Tocantins, Brazil. In one outbreak, 41 predominantly young bovines were affected, with 2-3 deaths in some cases. The animals showed neurological signs of incoordination, blindness, and recumbency, with death occurring within approximately 4-5 d. At necropsy, hyperemia and leptomeningeal hemorrhages were observed in the brain. Histology revealed more intense lesions in the rostral portions of the brain, mainly affecting the frontoparietal cerebral cortex, with nonsuppurative encephalitis and meningitis, glial nodules, neuronophagia, and eosinophilic intranuclear inclusion bodies in the astrocytes and neurons. This study shows the presence of bovine herpesvirus in Tocantins, probably the highly neurotropic type 5 strain, and emphasizes its importance in the differential diagnosis of bovine neuropathies.","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"46 ","pages":"e004023"},"PeriodicalIF":0.0,"publicationDate":"2024-01-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10829934/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139652312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Illustrated technique of superficial lymphadenectomy of dogs and cats: preliminary study. 猫狗浅表淋巴腺切除术图解技术：初步研究。

Brazilian journal of veterinary medicine Pub Date : 2024-01-17 eCollection Date: 2024-01-01 DOI: 10.29374/2527-2179.bjvm004823

Matheus Junger do Brasil, Clara Villela Castello Branco, Naomi Cappelli, Joice Bento da Silva, Rosaura Leite Rodrigues, Maria Eduarda Dos Santos Lopes Fernandes

{"title":"Illustrated technique of superficial lymphadenectomy of dogs and cats: preliminary study.","authors":"Matheus Junger do Brasil, Clara Villela Castello Branco, Naomi Cappelli, Joice Bento da Silva, Rosaura Leite Rodrigues, Maria Eduarda Dos Santos Lopes Fernandes","doi":"10.29374/2527-2179.bjvm004823","DOIUrl":"10.29374/2527-2179.bjvm004823","url":null,"abstract":"Superficial lymphadenectomy is an easy-to-perform and cost-effective routine technique. Despite its simplicity, it remains underutilized in veterinary medicine, with most practitioners being oncological surgeons. This study aims to enhance accessibility to the surgical procedure by providing anatomical representations of superficial lymphadenectomy in the carcasses of dogs and cats. A preliminary study involving two canines and two felines was conducted, with each group comprising a dog and a cat. Group A was designated to superficial lymphadenectomy techniques to create an illustrated step-by-step procedure, while group B underwent anatomical dissection to expose lymph nodes and their adnexa. The approach to superficial lymph nodes in dogs and cats is simple, allowing for the demonstration of superficial lymphadenectomy techniques in the corpses of dogs and cats without complications. This includes the dissection and presentation of anatomical structures adjacent to the lymph nodes. In conclusion, the techniques applied to subjects in groups A and B proved effective, successfully demonstrating and excising all superficial lymph nodes in the corpses of dogs and cats. These findings suggest that the developed set of techniques developed for lymph node excision holds promise for safe and effective application in live animals.","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"46 ","pages":"e004823"},"PeriodicalIF":0.0,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10811725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139572404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of RNA-seq to identify genes encoding cytokines and chemokines activated following uptake and processing a candidate peptide vaccine developed against Mycobacterium avium subsp. paratuberculosis. 利用 RNA-seq 鉴定编码细胞因子和趋化因子的基因，这些基因在吸收和处理针对副结核分枝杆菌亚种开发的候选多肽疫苗后被激活。

Brazilian journal of veterinary medicine Pub Date : 2024-01-16 eCollection Date: 2024-01-01 DOI: 10.29374/2527-2179.bjvm002723

Michelle Athena Decourcey, William Charles Davis, Cleverson de Souza

{"title":"Use of RNA-seq to identify genes encoding cytokines and chemokines activated following uptake and processing a candidate peptide vaccine developed against Mycobacterium avium subsp. paratuberculosis.","authors":"Michelle Athena Decourcey, William Charles Davis, Cleverson de Souza","doi":"10.29374/2527-2179.bjvm002723","DOIUrl":"10.29374/2527-2179.bjvm002723","url":null,"abstract":"Analysis of the primary and recall responses to a membrane molecule (MMP), encoded by MAP2121c demonstrated that tri-directional signaling between the antigen-presenting cell (APC), CD4 and CD8 is essential for eliciting a CD8 cytotoxic T cell (CTL) response against Mycobacterium avium subsp. paratuberculosis. As reported here, RNA-sequencing was used to initiate the characterization of the signaling pathways involved in eliciting the development of CD8 CTL, starting with the characterization of the activation status of genes in monocyte-derived macrophages (MoMΦ) following uptake and processing MMP for the presentation of antigenic epitopes to CD4 and CD8 T cells. Activation status was compared with the uptake and processing of LPS, a nonspecific stimulator of macrophages. 1609 genes were identified that were upregulated, and 1277 were downregulated three hours after uptake and processing MMP. No significant difference was observed in the cytokine genes selected for analysis of the signaling that must occur between APC, CD4, and CD8 for the development of CTL. The initial observations indicate screening of the transcriptome should include genes involved in signaling between APC and CD4, and CD8 regardless of their activation status. Four genes of interest in this study, IL12A, IL12B, IL15, and IL23A, were not significantly different from control values. The initial studies also indicate MoMΦ can be included with dendritic cells and monocyte-derived dendritic cells for further analysis of the tri-directional signaling required for the development of CTL.","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"46 ","pages":"e002723"},"PeriodicalIF":0.0,"publicationDate":"2024-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10811724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139572406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical and laboratory findings of hookworms (Ancylostoma spp.) in naturally infected adult dogs. 成年犬自然感染钩虫（钩虫属）的临床和实验室结果。

Brazilian journal of veterinary medicine Pub Date : 2024-01-13 eCollection Date: 2024-01-01 DOI: 10.29374/2527-2179.bjvm007724

Bruna Dos Santos, Naiara Mirelly Marinho da Silva, Silvia Eugenia Vargas Mora, André Augusto Justo, Elizabeth Moreira Dos Santos Schmidt, Regina Kiomi Takahira

{"title":"Clinical and laboratory findings of hookworms (Ancylostoma spp.) in naturally infected adult dogs.","authors":"Bruna Dos Santos, Naiara Mirelly Marinho da Silva, Silvia Eugenia Vargas Mora, André Augusto Justo, Elizabeth Moreira Dos Santos Schmidt, Regina Kiomi Takahira","doi":"10.29374/2527-2179.bjvm007724","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm007724","url":null,"abstract":"Intestinal parasites of the genus Ancylostoma are the most prevalent in coproparasitological examinations and necropsies of dogs in Brazil. Although adult dogs often remain asymptomatic when infected, there is limited published information concerning the laboratory and clinical findings and severity of infection in symptomatic adult dogs. Therefore, this study aimed to characterize the clinical and laboratory findings of adult Ancylostoma-infected dogs. Data were obtained by surveying the medical records of dogs treated at a University Veterinary Hospital from June 2009 to June 2019. Of 243 cases, 30 met the selection criteria. The most frequent clinical signs were diarrhea and hematochezia (13/30 each - 43.3%), and the most common laboratory findings were eosinophilia (13/30 - 43.3%), increased RDW (6/29 - 20.7%), and hypoalbuminemia (5/21 - 23.8%). Dogs with Ancylostoma spp. presented an increased proportion of high RDW (P= 0.010) compared with non-parasitized dogs. Dogs with higher fecal egg scores (n= 18) showed significantly lower RBC (6.26 ± 0.64 vs 7.24 ± 0.74 106/µL, P< 0.001) and albumin levels (2.8 ± 0.5 vs 3.2 ± 0.4 g/dL, P= 0.021), and an increased eosinophil count (1.42 ± 0.95 vs 0.58 ± 0.47 103/µL, P= 0.003) compared with the control group. Fecal egg scores exhibited a weak correlation with eosinophils (r = 0.42, P= 0.002) and a moderate correlation with RBC (r = -0.52, P< 0.001). In conclusion, despite relevant clinical signs, the laboratory findings were indicative of mild alterations in adult dogs, especially in those with lower scores of eggs in coproparasitological tests.","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"46 ","pages":"e007724"},"PeriodicalIF":0.0,"publicationDate":"2024-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11734537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0