Brazilian journal of veterinary medicine最新文献

{"title":"Evaluation of DNA extraction from different canine lymphoma sample types for PARR analysis.","authors":"Vinício Cascardo, Carla Beatriz Ventura Leite, Matheus de Souza Santana, Huarrisson Azevedo Santos, Thiago Souza Costa, Andresa Guimarães","doi":"10.29374/2527-2179.bjvm007926","DOIUrl":"10.29374/2527-2179.bjvm007926","url":null,"abstract":"<p><p>This study evaluated the performance of DNA extraction using the phenol-chloroform method across different types of canine lymphoma samples, with the aim of applying it to PARR. A total of 48 samples previously diagnosed as lymphoma were analyzed, including lymph node aspirates, cytological slides, andparaffin-embedded tissue blocks. DNA concentration was measured by spectrophotometry, and DNA suitability was confirmed by conventional PCR targeting a lymphocyte-specific endogenous control. No statistically significant differences were observed among the groups regarding DNA concentration (p > 0.05), with the following pairwise comparisons: paraffin-embedded tissue versus aspirate (p = 0.6827), aspirate versus cytological slide (p = 0.2242), and paraffin-embedded tissue versus cytological slide (p = 0.1173). All samples showed successful amplification, and the method demonstrated consistent performance, enabling the use of a single extraction protocol applicable to all three sample types, thereby showing potential as an accessible and standardizable approach for preparing samples for the PARR assay.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"48 ","pages":"e007926"},"PeriodicalIF":0.0,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13644735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149139533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anatomopathological features of uterine tube adenoma in a female dog.","authors":"Lorrayne Dos Santos Miranda, Marina Possa Dos Reys, Lara Coslop Comerio, Rodrigo Viana Sepulveda, Lidianne Narducci Monteiro, Geovanni Dantas Cassali, Mayra Cunha Flecher","doi":"10.29374/2527-2179.bjvm010626","DOIUrl":"10.29374/2527-2179.bjvm010626","url":null,"abstract":"<p><p>The uterine tube is part of the female reproductive system responsible for connecting the ovary to the uterus. Neoplasms originating from the uterine tube are rare in both animals and humans, with few reports available in the veterinary literature. A 7-year-old mixed-breed bitch was presented with clinical signs of lethargy, weight gain, and abdominal distension. Ultrasonography and computed tomography revealed a large abdominal mass. The patient underwent exploratory laparotomy for surgical excision of the mass, which was found adhered to the right ovary. Grossly, the mass measured 27.5 × 22.5 × 5.5 cm, had a soft consistency, irregular contours, reddish coloration, and a papillary appearance. Histopathological evaluation revealed well-differentiated papillary projections lined by ciliated columnar epithelial cells, with positive expression of estrogen and progesterone receptors and low Ki-67 labeling index, supporting the diagnosis of uterine tube adenoma. Although these tumors may reach considerable dimensions, they are generally associated with a favorable prognosis. In the present case, the diagnosis was established based on the correlation of clinical, morphological, and histopathological findings.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"48 ","pages":"e010626"},"PeriodicalIF":0.0,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13644734/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149139474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unusually long-term survival in a feline with Fibrodysplasia ossificans progressiva (FOP-like) disease without <i>ACVR1</i> gene mutations.","authors":"Claudia de Aurrecoechea, Sebastián Fernández Barrios, Rody Artigas","doi":"10.29374/2527-2179.bjvm005726","DOIUrl":"10.29374/2527-2179.bjvm005726","url":null,"abstract":"<p><p>Fibrodysplasia ossificans progressiva (FOP) is a rare genetic disorder characterized by heterotopic ossification within the connective tissues that affects the muscles, tendons, and joint capsules. It is an autosomal inherited condition caused by mutations in ACVR1, which is associated with ectopic bone formation. A 5-year-old neutered male outdoor cat, clinically diagnosed with FOP-like disease three years prior, presented for follow-up evaluation owing to an unusually long survival. New blood samples and radiographs were obtained to assess disease progression and investigate the patient's genetic background, with the aim of describing this case's clinical evolution and new genetic findings, and sharing the patient's exceptionally rare long-term survival with peers. Radiographic evaluation revealed severe progression of osteoarticular disease, partial to total resorption of previously deposited mineralized tissue, marked hypertrophy of the limb musculature, and an overall increase in radiographic muscular density. Genetic analysis confirmed the absence of the ACVR1 mutation. This is the first reported case of FOP-like disease in a cat in Uruguay, with no ACVR1 mutation detected and an unusually long survival of more than three years after diagnosis.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"48 ","pages":"e005726"},"PeriodicalIF":0.0,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13644736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149139641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of the continuous rate infusion of propofol with or without fentanyl on ruminal motility in calves.","authors":"Guilherme Lopes da Silva, Maurício Deschk, Thomas Alexander Trein, Marcelo Augusto de Araújo, Bruna de Moraes Martins Games, Celso Antônio Rodrigues, Paulo Sérgio Patto Dos Santos","doi":"10.29374/2527-2179.bjvm010326","DOIUrl":"10.29374/2527-2179.bjvm010326","url":null,"abstract":"<p><p>This study aimed to evaluate ruminal motility before, during, and after propofol anesthesia with or without fentanyl in Holstein calves by investigating effects of these anesthetic protocols on rumen function and cardiorespiratory variables. This study employed a randomized crossover design with a 1-week washout period between treatments and included eight calves aged 6-12 months and weighing 100-180 kg. Animals received either propofol alone (propofol group [PG]) or propofol with fentanyl (propofol-fentanyl group [FG]), alternating the order of treatments between groups. Anesthesia was induced with propofol (5.0 mg/kg IV) in the PG and with propofol (4.0 mg/kg IV) and fentanyl (0.001 mg/kg IV) in the FG. Next, anesthesia was maintained for 60 min by the continuous infusion of propofol (0.6 mg/kg/min IV) alone in the PG or combined with fentanyl (0.001 mg/kg/h IV) in the FG. Cardiorespiratory variables were recorded at baseline and every 15 min during anesthesia. Ruminal motility was assessed before fasting, after fasting, during anesthesia, and throughout recovery until contractions returned to baseline values. We noted that the median (minimum/maximum) ruminal normalization time did not differ significantly between the PG (35 [10/50] min) and FG (45 [20/120] min) (P = 0.19). Food withholding and handling-related stress reduced ruminal motility in both groups. Both protocols completely suppressed ruminal contractions during anesthesia, with contractions beginning to return approximately 10 min after the end of infusion in the PG and 20 min in the FG. Furthermore, propofol caused hypoventilation and respiratory acidosis while maintaining hemodynamic variables within clinically acceptable limits. In summary, fentanyl addition was not associated with a statistically significant prolongation of ruminal-motility recovery, although the wider range of the ruminal normalization time observed in the FG suggests individual variability, warranting further investigation.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"48 ","pages":"e010326"},"PeriodicalIF":0.0,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13644737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149139530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology of the major salivary glands in ocelots (<i>Leopardus pardalis</i>): description of 02 cases.","authors":"Letícia Victal Pereira, Anieli Vidal Stocco, Raquel Batista Junger de Carvalho, Carlos Augusto Santos Sousa, Paulo Souza, Marcelo Abidu-Figueiredo","doi":"10.29374/2527-2179.bjvm006126","DOIUrl":"10.29374/2527-2179.bjvm006126","url":null,"abstract":"<p><p>The ocelot (<i>Leopardus pardalis</i>) is the largest species of its genus and, despite its wide distribution throughout the Americas, it is included on the main list of endangered species. Its conservation has been widely studied, but there is a lack of studies regarding its morphology. The morphology, measurements, and location of the salivary glands in wild felids are fundamental, as they provide basic information for understanding the biology of these species. This study was approved by the Research Ethics Committee of the Federal Rural University of Rio de Janeiro (018/2017). Anatomical dissections were performed on the cadavers of two adult female ocelots. In the specimens studied, the parotid (PG), mandibular (MG), molar (MolG), sublingual (SLG), and zygomatic (ZG) salivary glands were identified in both antimeres. The length, width, thickness, and weight of the glands were measured, revealing individual variations among the analyzed specimens. Information on the anatomy of salivary glands in wild animals is scarce. The description of these glands in wild felids is important, as it may facilitate clinical treatments and provide information for professionals working in zoos and conservation units.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"48 ","pages":"e006126"},"PeriodicalIF":0.0,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13644739/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149139633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hidden epidemic in the concrete jungle: habitat fragmentation and rising snakebite occurrence in São Paulo, a tropical megacity.","authors":"Renan de Oliveira Nogueira, Beatriz Rodrigues da Cruz, Pedro Augusto Pires Brandão, Herbert Sousa Soares, Arlei Marcili, Guilherme José da Costa Silva","doi":"10.29374/2527-2179.bjvm002926","DOIUrl":"10.29374/2527-2179.bjvm002926","url":null,"abstract":"<p><p>This study analyzes 13,111 snakebite cases (2010-2023) in São Paulo, Brazil, correlating them with deforestation patterns using SINAN and MapBiomas data. Snakebite occurrence was concentrated in the southern zone, particularly in the Parelheiros district, an area characterized by intense urban expansion, fragmented forest remnants, and high social vulnerability. <i>Bothrops</i> species were responsible for 81.5% of envenomings, with incidents concentrated in three districts bordering fragmented forests. Annual deforestation fluctuated (43.1-321.2 ha), but snakebite patterns showed unique temporal dynamics, including a 2012 outbreak potentially linked to habitat fragmentation from the Rodoanel highway expansion. Intermediate deforestation zones (average of 137.5 ha/year) experienced the highest risks, suggesting edge effects and rodent proliferation as key mediators. The results demonstrate how urban expansion in biodiversity hotspots exacerbates human-wildlife conflict, highlighting the need for integrated prevention strategies in urban-forest interface zones.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"48 ","pages":"e002926"},"PeriodicalIF":0.0,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13644740/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149139476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the response of creole cows (<i>Bos taurus</i>) to two fixed-time artificial insemination protocols in the High-Andean region of Ayacucho, Peru.","authors":"Mijaíl Contreras Huamaní, César Jorge Mendoza Leiva, Hamilton Guzman Santaria, Walter Palomino-Guerrera, Hurley Abel Quispe-Ccasa","doi":"10.29374/2527-2179.bjvm000326","DOIUrl":"10.29374/2527-2179.bjvm000326","url":null,"abstract":"<p><p>Unlike specialized breeds, High-Andean Creole cattle have developed a remarkable adaptability and hardiness to challenging high-altitude conditions, albeit at the expense of productivity. However, the efficiency of their reproductive performance under estrus synchronization protocols for fixed-time artificial insemination (FTAI) remains scarcely addressed. This study aimed to evaluate the response of Creole cows to two FTAI protocols in the districts of Chuschi (Cangallo) and Chipao (Lucanas), Ayacucho, Peru. From a sample of 60 Creole cows (identified by phenotype and coat color), with uniform body condition scores and management system, 35 were synchronized using a three-handling protocol (T1: estradiol cypionate and Bovine Intravaginal device (BID) removal on day 8), and 25 using a four-handling protocol (T2: BID removal on day 7 and estradiol benzoate on day 8). The cows were inseminated using thawed semen from Fleckvieh and Brown Swiss bulls, the latter belonging to the genetic nucleus of the Instituto Nacional de Innovación Agraria. Estrus detection rates ranged from 88.6% to 80.0%, yielding pregnancy rates of 57.1% and 56.0% for T1 and T2, respectively (p>0.05). No significant effects of protocol, age, number of calvings, body condition score, or bull breed were found on estrus detection, pregnancy, or calf sex (p>0.05). However, cows with a corpus luteum had 7.1-fold higher odds of pregnancy than those presenting only follicles (p<0.05). In conclusion, both FTAI protocols demonstrated comparable reproductive performance; however, the three-handling protocol with estradiol cypionate emerges as the preferred option due to its lower cost and greater operational simplicity under challenging High-Andean conditions.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"48 ","pages":"e000326"},"PeriodicalIF":0.0,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13644738/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149139557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Total ear canal ablation for the treatment of auricular hemangiosarcoma in a cat: case report.","authors":"Júlia Vaz Feio, Elaine Lopes de Carvalho","doi":"10.29374/2527-2179.bjvm012626","DOIUrl":"10.29374/2527-2179.bjvm012626","url":null,"abstract":"<p><p>Ear neoplasms are growths that commonly affect light-coated, unpigmented felines exposed to prolonged sunlight. Another factor associated with these growths is the constant tissue trauma caused by intense pruritus in cases of chronic otitis. Any tissue making up the structure of the pinna can undergo changes and initiate pathogenic cell proliferation. In this context, cutaneous hemangiosarcomas may arise from alterations in the ear's vascular endothelium, albeit less frequently. This report describes the case of a 12-year-old, white, mixed-breed cat with a history of prior pinnectomy and local recurrence. To treat the new lesion, total ear canal ablation combined with cryotherapy of the surgical bed was selected to maximize recurrence-free survival. Histopathological analysis of the surgical specimen confirmed a moderate grade hemangiosarcoma associated with chronic otitis, with clear margins. The patient was referred to for oncological follow-up and remains free of recurrence one year after the procedure.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"48 ","pages":"e012626"},"PeriodicalIF":0.0,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13618361/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of the subcutaneous ureteral bypass system (SIDUS) in dogs and cats: a case series study.","authors":"Letícia Alves Wrobel, Fabiana Antunes Andrade, Eloísa Muehlbauer, Mariana Etchepare, Renato Nisihara","doi":"10.29374/2527-2179.bjvm005926","DOIUrl":"10.29374/2527-2179.bjvm005926","url":null,"abstract":"<p><p>Subcutaneous ureteral bypass (SIDUS) has become an established surgical alternative for the management of ureteral obstruction in cats and dogs, providing urinary diversion and facilitating renal decompression. This retrospective cohort study evaluated the clinical, biochemical, and microbiological outcomes of 17 animals that underwent SIDUS implantation and were followed for up to 90 days. Data were retrospectively obtained from medical records, including clinical assessments, laboratory tests, and urine cultures. Serum creatinine concentrations decreased significantly from a preoperative median of 10.0 to 6.0 mg/dL at 24 h after surgery (<i>p</i> < 0.001) and remained at a median of 1.8 mg/dL at 60 and 90 days. Urea and phosphorus concentrations showed similar reductions following implantation. Leukocyte counts exhibited mild postoperative variation, consistent with a possible inflammatory response. Urine cultures were predominantly negative throughout follow-up, and positive cultures were sporadic and not accompanied by reported clinical signs or deterioration of laboratory parameters. Despite the small sample size and limited follow-up period, these findings indicate substantial improvement in renal biochemical parameters following SIDUS implantation, with few clinically relevant urinary tract infections observed during the study period.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"48 ","pages":"e05926"},"PeriodicalIF":0.0,"publicationDate":"2026-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13618360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ERRATUM: Characterization of βlactoglobulin and κcasein genotypes PCR-RFLP in dairy cattle from Panama.","authors":"","doi":"10.29374/2527-2179.bjvm013626","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm013626","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.1590/abd1806-4841.20142998.].</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"48 ","pages":"e013626"},"PeriodicalIF":0.0,"publicationDate":"2026-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13577604/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148978959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}