Brazilian journal of veterinary medicine最新文献

{"title":"Pulmonary aspergillosis in a horse: a case report.","authors":"Ubiratan Pereira Melo, Cíntia Ferreira, Suziane Wilma Mota Barreto","doi":"10.29374/2527-2179.bjvm004723","DOIUrl":"10.29374/2527-2179.bjvm004723","url":null,"abstract":"<p><p>Respiratory diseases considerably affect equine athletes, being the second most common cause of poor performance. Among these diseases, fungal pneumonia in horses, caused specifically by <i>Aspergillus</i> spp., is relatively rare but potentially fatal. Fungal pneumonia typically affects horses exposed to fungal elements due to environmental factors, immunosuppression, or previous debilitating illnesses. Treatment is complex, with minimal success due to late diagnosis and serious concomitant underlying diseases. The choice of medication depends on the site of infection, the fungal species involved, and financial considerations. This report describes a case of pulmonary aspergillosis diagnosed in a 10-year-old castrated Quarter Horse. Transtracheal lavage revealed fungal elements characteristic of <i>Aspergillus fumigatus</i>. Treatment with dexamethasone, bromhexine hydrochloride, and itraconazole led to a successful recovery. The diagnosis of equine aspergillosis is challenging because its clinical signs overlap with other respiratory diseases. Fungal infections like aspergillosis are gaining attention in the equine health scene. Early and accurate diagnosis is crucial to avoid unnecessary use of antibiotics and prevent antimicrobial resistance. Furthermore, veterinarians and horse handlers must be aware of the risks of spreading aspergillosis to humans, emphasizing preventative measures and respiratory protection.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"46 ","pages":"e004723"},"PeriodicalIF":0.0,"publicationDate":"2024-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10811723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139572405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Addition of synthetic polymer in the freezing solution of mesenchymal stem cells from equine adipose tissue as a future perspective for reducing of DMSO concentration.","authors":"Cátia Nascimento, Márcia Viviane Alves Saraiva, Vitoria Mattos Pereira, Danielle Cristina Calado de Brito, Francisco Léo Nascimento de Aguiar, Benner Geraldo Alves, Kelly Cristine Santos Roballo, José Ricardo de Figueiredo, Carlos Eduardo Ambrósio, Ana Paula Ribeiro Rodrigues","doi":"10.29374/2527-2179.bjvm002523","DOIUrl":"10.29374/2527-2179.bjvm002523","url":null,"abstract":"<p><p>The regenerative therapies with stem cells (SC) has been increased by the cryopreservation, permitting cell storage for extended periods. However, the permeating cryoprotectant agents (CPAs) such as dimethylsulfoxide (DMSO) can cause severe adverse effects. Therefore, this study evaluated equine mesenchymal stem cells derived from adipose tissue (eAT-MSCs) in fresh (Control) or after slow freezing (SF) in different freezing solutions (FS). The FS comprise DMSO and non-permeating CPAs [Trehalose (T) and the SuperCool X-1000 (X)] in association or not, totalizing seven different FS: (DMSO; T; X; DMSO+T; DMSO+X; T+X, and DMSO+T+X). Before and after cryopreservation were evaluated, viability, colony forming unit (CFU), and cellular differentiation capacity. After freezing-thawing, the viability of the eAT-MSCs reduced (P< 0.05) in all treatments compared to the control. However, the viability of frozen eAT-MSCs in DMSO (80.3 ± 0.6) was superior (P<0.05) to the other FS. Regarding CFU, no difference (P>0.05) was observed between fresh and frozen cells. After freezing-thawing, the eAT-MSCs showed osteogenic, chondrogenic, and adipogenic lineages differentiation potential. Nonetheless, despite the significative reduction in the osteogenic differentiation capacity between fresh and frozen cells, no differences (P > 0.05) were observed among FS. Furthermore, the number of chondrogenic differentiation cells frozen in DMSO+X solution reduced (P<0.05) comparing to the control, without differ (P>0.05) to the other FS. The adipogenic differentiation did not differ (P>0.05) among treatments. In conclusion, although these findings confirm the success of DMSO to cryopreserve eAT-MSCs, the Super Cool X-1000 could be a promise to reduce the DMSO concentration in a FS.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e002523"},"PeriodicalIF":0.0,"publicationDate":"2023-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10756151/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139076053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluctuations of antibody serum titers for <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> in naturally infected crossbred cows during gestation.","authors":"Uillians Volkart de Oliveira, Vanessa Carvalho Sampaio de Magalhães, Sônia Carmen Lopo Costa, Ivan Bezerra Allaman, Alexandre Dias Munhoz","doi":"10.29374/2527-2179.bjvm003023","DOIUrl":"10.29374/2527-2179.bjvm003023","url":null,"abstract":"<p><p>This study aimed to assess the fluctuations of antibody serum titers for <i>Toxoplasma gondii</i> and <i>Neospora caninum</i> in naturally infected crossbred cows during gestation and to investigate transplacental transmission of <i>T. gondii</i>; 51 cows were monitored during pregnancy by monthly serologic testing by indirect fluorescent antibody test. 33 cows were seronegative for both <i>N. caninum</i> and <i>T. gondii</i>, 10 were seropositive only for <i>N. caninum</i>, 5 were seropositive only for <i>T. gondii,</i> and 3 were seropositive for both <i>N. caninum</i> and <i>T. gondii</i>. In both protozoan infections, great variation in antibody levels in pregnant cows was observed, and there was significant increase (p<0.05) in the comparison between the averages of serological titration per trimester. There was a significant correlation (p<0.05) between month and probability of seropositivity for <i>T. gondii</i>. We conclude that pregnancy influences antibody titers of crossbred cows naturally infected with <i>N. caninum</i> and/or <i>T. gondii</i>, and that serologic testing for <i>T. gondii</i> in pregnant cows from the sixth month of gestation onward may decrease the number of false negative results.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e003023"},"PeriodicalIF":0.0,"publicationDate":"2023-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10756150/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139076054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gasless laparoscopic renal biopsy in canine cadavers: a preclinical study.","authors":"Vanessa Milech, Bernardo Nascimento Antunes, Pâmela Caye, Hellen Fialho Hartmann, Marcella Teixeira Linhares, Vinícius da Silva Cadiñanos, Thiago Rodrigues da Cunha, Helena Castro Diniz, Maurício Veloso Brun","doi":"10.29374/2527-2179.bjvm000523","DOIUrl":"10.29374/2527-2179.bjvm000523","url":null,"abstract":"<p><p>In this study we aimed to analyze the feasibility of the gasless renal biopsy technique in canine cadavers. The cadavers were randomly divided into two groups: laparoscopic GCG, in which gasless laparoscopy was performed and GCP, laparoscopy with pneumoperitoneum was performed. The procedures were randomly performed on the right and left kidneys. The total surgical time, procedural steps, and intraoperative complications were recorded. The degree of difficulty of the surgical approaches was evaluated by the surgeon, assistant, and external evaluators. Renal samples were evaluated for quality, number of glomeruli, and proportion of renal cortex. The total operative time was higher in the GCG group than in the GCP group (p < 0.01). Additionally, positioning of the second portal and platform positioning took longer than the other steps. The surgical groups differed from each other in the Likert scale values for almost all the parameters regarding the difficulty of the surgical approaches (p < 0.05), with higher scores in the GCG group than in the GCP group. Based on the video recordings, the GCP group had higher scores than the GCG group for degrees of difficulty of the approach (p < 0.05). Renal histological parameters were similar between the surgical groups and surgical sides. Our study findings indicate that the proposed <i>gasless</i> renal biopsy technique is feasible. The longer operative time and technical difficulties in the gasless approach did not affect the quality of the renal specimens.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e000523"},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10750501/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139041052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dioctophimosis in a community dog from a public park in the city of Curitiba-PR: a case report from the perspective of One Health.","authors":"Vitor Campos Assumpção de Amarante, Vivien Midori Morikawa, Antonio Waldir Cunha da Silva, Dirciane Floeter","doi":"10.29374/2527-2179.bjvm004923","DOIUrl":"10.29374/2527-2179.bjvm004923","url":null,"abstract":"<p><p>The present study describes from the holistic One Health perspective a case report of dioctophimosis in a community dog (defined as stray dogs managed by the local community) residing in a park in the city of Curitiba-PR, considering the multispecies collectives directly affected by the dynamics of this dangerous zoonosis. The work underlines the importance of the community dog program of the Rede de Proteção Animal, demonstrating the health barrier function of these dogs. Despite being a cosmopolitan zoonosis, dioctophimosis is of particular importance in regions of the developing world, including Latin America, because of large populations of stray animals and social inequalities. Public policies focused on raising awareness among the general population about this parasitic disease and its risks and the restraint of actions that determine risks of infection for humans and companion animals are fundamental to a One Health approach to prevent this zoonosis.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e004923"},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10750503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139041050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Occurrence of <i>Toxoplasma gondii</i> and other gastrointestinal parasites in free-roaming cats from the Rio de Janeiro zoo.","authors":"Igor Falco Arruda, Raissa Cristina Ferreira Ramos, Alynne da Silva Barbosa, Ginette Villar-Echarte, Anderson Mendes Augusto, Fernando Troccoli, Maria Regina Reis Amendoeira","doi":"10.29374/2527-2179.bjvm006023","DOIUrl":"10.29374/2527-2179.bjvm006023","url":null,"abstract":"<p><p>This communication aimed to determine the frequency of infection by <i>T. gondii</i> and other gastrointestinal parasites in free-living cats captured in BioParque do Rio (Fundação RioZoo), Brazil. To this end, 58 blood and 51 fecal samples were collected from 68 cats from July 2019 to September 2020. The serum obtained was analyzed by indirect fluorescent antibody test for anti-<i>T. gondii</i> IgG. Fecal samples were examined by microscopic parasitological techniques. Of the total, 10.3% of the animals presented anti-<i>T. gondii</i> IgG. Parasitic structures were observed in 39.2% of the fecal samples. Hookworms were the most detected parasites (19.6%) followed by <i>Cystoisospora</i> sp. (11.7%), <i>Dipylidium caninum</i> (7.8%) and <i>Toxocara cati</i> (3.9%). These results indicated the exposure of this population of stray cats to potentially zoonotic parasites, which in addition to causing possible damage to domestic and wild animal health, also pose risks to public health.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e006023"},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10750499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139041053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endoparasites of <i>Leopardus pardalis</i> (Carnivora, Felidae) rescued in the State of Rio de Janeiro.","authors":"Gabriela Pereira Salça de Almeida, So Yin Nak, Gabriel Alcides Capucho, Brena Gava Guimarães, Diefrey Ribeiro Campos, Debora Azevedo Borges, Daniel de Almeida Balthazar, Thais Ribeiro Correia","doi":"10.29374/2527-2179.bjvm006123","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm006123","url":null,"abstract":"<p><p>Wild cats play an important role as top predators in the food chain and act as ecosystem regulators. However, in recent decades, many studies have demonstrated the potential effects of parasitic diseases on wild carnivore populations, including cats. This study reports on the endoparasites found in an injured and rescued specimen of <i>Leopardus pardalis</i> in the state of Rio de Janeiro. Fecal samples were collected and processed using five coproparasitological techniques: a simple flotation centrifugation, a zinc sulfate flotation centrifugation, a formalin-ether sedimentation centrifugation, a conical centrifuge tube technique, and a modified Ziehl-Neelsen staining technique for fecal smears. Helminth eggs belonging to the families Trichuridae and Diphyllobothriidae and the genus <i>Toxocara</i> were found in both sedimentation flotation techniques. Protozoan oocysts belonging to the genus <i>Cryptosporidium</i> were identified by modified Ziehl-Neelsen staining. These findings show that ocelots can harbor potentially zoonotic and pathogenic endoparasites. Further studies on the helminths and protofauna of these animals are necessary.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e006123"},"PeriodicalIF":0.0,"publicationDate":"2023-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10773826/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139405513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In vitro</i> evaluation of the activity of Cinnamaldehyde as an inhibitor of the biological cycle of <i>Ctenocephalides felis felis</i>.","authors":"Cecília Lopes da Conceição, Ester Oliozi Marré, Ygor Henrique da Silva, Larissa de Oliveira Santos, Brena Gava Guimarães, Taynara Monsores E Silva, Matheus Eduardo Costa da Silva, Diefrey Ribeiro Campos, Fabio Barbour Scott, Katherina Coumendouros","doi":"10.29374/2527-2179.bjvm005123","DOIUrl":"10.29374/2527-2179.bjvm005123","url":null,"abstract":"<p><p>The objective of this work was to evaluate the <i>in vitro</i> activity of cinnamaldehyde as an inhibitor of the biological cycle of <i>Ctenocephalides felis felis</i>. Tests were carried out with six repetitions containing 10 eggs of <i>C. felis felis</i> together with 0.5g of diet and filter paper discs with area of 23.76 cm² impregnated with different concentrations of cinnamaldehyde (6; 8; 10; 12; 14; 16; 18; 20; 24 and 28 µg.cm^-2) in Petri dishes and incubated in a climate-controlled chamber at 27 ± 1 °C and RH 75 ± 10%, for 30 days. The tests included a placebo group containing the diluent (acetone) and a negative control group, without treatment. At the end of 30 days, the percentage of inhibition of adult emergence/mortality was calculated, as well as the LC₅₀ and LC₉₀. Inhibition of adult emergence greater than 50% was observed from a concentration of 10 µg.cm^-2 and 100% inhibition from a concentration of 14 µg.cm^-2, obtaining LC₅₀ and LC₉₀ results of 8.75 and 13.57 µg.cm^-2, respectively. We concluded that the volatile compound cinnamaldehyde is effective <i>in vitro</i> as an inhibitor of the biological cycle of <i>C. felis felis</i>.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e005123"},"PeriodicalIF":0.0,"publicationDate":"2023-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10750500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139041049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Flea parasitism in wild mammals in the metropolitan region of Sorocaba, São Paulo, Brazil.","authors":"Rodrigo Hidalgo Friciello Teixeira, André Luiz Mota da Costa, Cassia Regina Ramos Gonzaga, Flora Nogueira Matos, Marina Alvarado de Medeiros, Shamira de Fátima Sallum Leandro, Ronnie Von Matheus, Maria Atalla Bellotti, Beatriz Freitas Lima, Cláudio Alessandro Massamitsu Sakamoto, Luciano Antunes Barros","doi":"10.29374/2527-2179.bjvm003523","DOIUrl":"10.29374/2527-2179.bjvm003523","url":null,"abstract":"<p><p>Fleas are eurixenous ectoparasites that can parasitize different species of warm-blooded animals, including humans, with the possibility of causing irritation and blood spoliation. They are vectors responsible for the transmission of numerous pathogens and have a wide geographical distribution, more frequently in warm regions. Domestic animals (dogs and cats) are preferred hosts, but parasitism can also occur in wild hosts, with a greater variety of parasitic species and strong interaction between these siphonapters and their hosts. During the period from January 2021 to June 2023, flea specimens were collected from wild animals coming from the metropolitan region of Sorocaba, São Paulo. Some of these animals were animals from the zoo's own stock, which were diagnosed with parasitism during routine examinations and others were rescued from the natural environment and sent to the zoo. The flea specimens collected were packed in alcohol 700 GL and sent for microscopic diagnosis. Four groups were diagnosed at the specific level: <i>Ctenocephalides felis</i>, <i>Rhopalopsyllus lutzi</i>, <i>R. lugrubis</i> and <i>Tunga penetrans,</i> and two groups as unidentified species, belonging to the genera <i>Rhopalopsyllus</i> and <i>Polygenis</i>. The parasitized animals were all mammals, belonging to eleven different species, distributed in the Orders Carnivora, Didelphimorphia, Rodentia and Cingulata. Studies on the parasitofauna of wild animals contribute to a greater knowledge about the distribution of parasitic agents and their relationships with the host species.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e003523"},"PeriodicalIF":0.0,"publicationDate":"2023-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10750502/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139041051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cavia porcellus</i> (Rodentia: Caviidae) as host for <i>Ctenocephalides felis felis</i> (Siphonaptera: Pulicidae) in artificially infestation.","authors":"Thalita Xavier de Araujo da Silva, Gabriela Pereira Salça de Almeida, Debora Azevedo Borges, Victor Elias Caceres Rios, Thaís Ribeiro Correia","doi":"10.29374/2527-2179.bjvm003123","DOIUrl":"https://doi.org/10.29374/2527-2179.bjvm003123","url":null,"abstract":"<p><p>The number of guinea pigs is increasing as pet and their domestication necessitates the study of their pathology and emerging diseases. This study aimed to evaluate guinea pigs' capacity to be infested by <i>Ctenocephalides felis felis</i> fleas, as it is a common parasite among pets that causes irritation, stress, and transmission of other pathogens. Seventeen guinea pigs were infested with <i>C. felis felis</i>. After 48 hours, the animals were combed, and the number of fleas was determined. Guinea pigs had a very low recovery (average of 5%), leading us to conclude that they are not good hosts for this species, despite some literature citing it as an accidental host if infested along with dogs or cats.</p>","PeriodicalId":72458,"journal":{"name":"Brazilian journal of veterinary medicine","volume":"45 ","pages":"e003123"},"PeriodicalIF":0.0,"publicationDate":"2023-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10718048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138811943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}