{"title":"Plant resources for immunonutrients and immunomodulators to combat infectious respiratory viral diseases: a review.","authors":"Sobha Kota, Anand Kumar Nelapati, Vayunandana Rao Govada","doi":"10.1007/s13205-024-04143-y","DOIUrl":"10.1007/s13205-024-04143-y","url":null,"abstract":"<p><p>Boosting the immune system has become a crucial aspect in the global battle against the COVID-19 pandemic and other similar infections to protect oneself against symptoms, especially in the prevention of viral infections of the lower respiratory tract. The importance of conducting more studies to create successful herbal formulations as infection prevention measures is emphasized in this review, which looks at the function of immune-boosting nutrients, medicinal plants, and herbal treatments. We reviewed and analyzed 207 studies published from 1946 to the present using reputable databases like Google Scholar, PubMed, and NCBI. The review examined 115 plant species in total and identified 12 key nutrients, including vitamins A, D, C, omega-3 fatty acids, iron, and zinc, while noting that four plant families, Rosaceae, Asteraceae, Amaryllidaceae, and Acanthaceae, show potential against respiratory infections like influenza, RSV, and SARS-CoV. To lower the risk of infection, it is recommended to consume nutritious meals that have immune-modulating qualities. Information on the bioactive components of medicinal herbs, spices, and plants that have been effective in treating respiratory viral infections and related conditions is compiled in this review, which highlights phytoactive substances with antibacterial and antiviral activity as effective modulators to lower the risk of infections. Furthermore, it is highlighted that ancient knowledge systems, like Ayurveda and Naturopathy, should be integrated to help develop new herbal formulations. To improve immunity and lessen vulnerability to serious respiratory infections, the results highlight the need for including immune-modulating foods and plant-based medicines into everyday routines.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"302"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568085/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
3 BiotechPub Date : 2024-12-01Epub Date: 2024-11-17DOI: 10.1007/s13205-024-04137-w
P Sujith, J Rajesh Singh, S Jayalakshmi, Karthikeyan Kandaswamy, Mohammed Rafi Shaik, Shaik Althaf Hussain, Zulhisyam Abdul Kari, Ajay Guru
{"title":"Metagenomics approaches in the discovery and development of new bioactive compound of 8-demethoxy-10-deoxysteffimycin from mangrove sediments.","authors":"P Sujith, J Rajesh Singh, S Jayalakshmi, Karthikeyan Kandaswamy, Mohammed Rafi Shaik, Shaik Althaf Hussain, Zulhisyam Abdul Kari, Ajay Guru","doi":"10.1007/s13205-024-04137-w","DOIUrl":"10.1007/s13205-024-04137-w","url":null,"abstract":"<p><p>A metagenomic library consisting of 15,000 clones was constructed from the mangrove sediment. An antimicrobially active clone from the metagenomic library PS49 was identified by function- based screening. This paper presents the results of the biochemical characterization and metagenomic library screening of the marine-derived antibiotic, 8-demethoxy-10-deoxysteffimycin. Plasmid libraries were constructed, and clones were produced using a metagenomic approach. Out of 15,000 clones, 81 clones were screened for antimicrobial activity, and five potential clones were selected. The activity of one clone was characterized and named as PS49. The bioactive compounds from the selected clone were checked for antimicrobial, antioxidant, and anticancer activities. The clone PS49 was tested against various pathogens including bacteria and fungi and it showed inhibitory effects against all the tested pathogens. The antimicrobially active fractions were then crystallized and subjected to spectroscopic analysis such as FTIR, NMR and LC-MS analysis. The substance from clone PS49 has finally been recognized, and the compound from clone PS49 has been identified as 8-demethoxy-10-deoxysteffimycin. The substances isolated from the PS49 clone exhibited strong anticancer activity against skin cancer-cell lines SK-MEL2. The compounds showed a reduction in cell viability with an increase in the compound concentration. The compounds obtained from clone PS49 showed an IC<sub>50</sub> value of 85 µg/ml.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"303"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11570573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142674806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
3 BiotechPub Date : 2024-12-01Epub Date: 2024-11-09DOI: 10.1007/s13205-024-04148-7
Ning Tang, Nuojin Geng, Xinhua Zhu
{"title":"Evaluation of Xihuang Pill in inducing pyroptosis in glioma cells through modulation of miR-21-5p.","authors":"Ning Tang, Nuojin Geng, Xinhua Zhu","doi":"10.1007/s13205-024-04148-7","DOIUrl":"https://doi.org/10.1007/s13205-024-04148-7","url":null,"abstract":"<p><p>This study aims to elucidate the mechanism by which Xihuang Pill induces pyroptosis in glioma cells via the regulation of miR-21-5p. Human glioma cell lines U-87 and LN-229 were used as experimental models to assess the effects of Xihuang Pill on glioma pyroptosis. Cells were incubated with Xihuang Pill extract at concentrations of 7.5, 15, and 30 µg/mL for 24 h, alongside transfection with miR-21-5p mimic, an overexpression vector for STAT3, or incubation with 50 µg/mL of the STAT3 activator Colivelin for 4 h. Cell viability was measured using the CCK-8 assay, apoptosis was detected by flow cytometry, and expression levels of p-STAT3/STAT3 and pyroptosis-related proteins were determined by Western Blot. Additionally, cleaved caspase-1 was assessed by immunofluorescence, miR-21-5p expression by qRT-PCR, and STAT3 binding to the miR-21-5p promoter region by ChIP and dual-luciferase reporter assays. Results showed that Xihuang Pill significantly reduced cell viability, increased apoptosis, and upregulated the expression of pyroptosis-related proteins such as NLRP3, IL-1β, cleaved caspase-1, and GSDMD-N, while reducing p-STAT3/STAT3 and miR-21-5p levels (P < 0.05). Xihuang Pill inhibited STAT3 activation, which modulated miR-21-5p expression by binding to its promoter region. Co-transfection with miR-21-5p mimic reversed the effect of Xihuang Pill on glioma pyroptosis (P < 0.05). In conclusion, Xihuang Pill promotes glioma cell pyroptosis through the STAT3/miR-21-5p pathway.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"295"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11550298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
3 BiotechPub Date : 2024-12-01Epub Date: 2024-11-04DOI: 10.1007/s13205-024-04133-0
Xiaofeng Wan, Qingmei Deng, Anling Chen, Xinhui Zhang, Wulin Yang
{"title":"Bioinformatics analysis and experimental validation of the oncogenic role of COL11A1 in pan-cancer.","authors":"Xiaofeng Wan, Qingmei Deng, Anling Chen, Xinhui Zhang, Wulin Yang","doi":"10.1007/s13205-024-04133-0","DOIUrl":"10.1007/s13205-024-04133-0","url":null,"abstract":"<p><p>The intricate expression patterns and oncogenic attributes of COL11A1 across different cancer types remain largely elusive. This study used several public databases (TCGA, GTEx, and CCLE) to investigate the pan-cancer landscape of COL11A1 expression, its prognostic implications, interplay with the immune microenvironment, and enriched signaling cascades. Concurrently, western blot analyses were performed to verify COL11A1 expression in lung adenocarcinoma (LUAD) cell lines and clinical samples. In addition, COL11A1 knockout cell lines were generated to scrutinize the functional consequences of COL11AI expression on cancer cell behavior by use MTT, colony formation, and scratch wound healing assays. A comprehensive database investigation revealed that COL11A1 was upregulated in a majority of tumor tissues and its expression was highly correlated with a patient's prognosis. Notably, genetic alterations in <i>COL11A1</i> predominantly occurred as mutations, while its DNA methylation status inversely mirrored gene expression levels across multiple promoter regions. Our findings suggest that COL11A1 helps to modulate the tumor immune landscape and potentially acts through the epithelial-mesenchymal transition (EMT) pathway to exert its oncogenic function. Western blot analyses further substantiated the specific upregulation of COL11A1 in LUAD cell lines and tissues, suggesting a close association with the EMT process. Ablation of COL11A1 in cancer cells significantly reduced their proliferative, clonogenic, and migratory abilities, underscoring the functional significance of COL11A1 in tumor cell behavior. Collectively, this research revealed the prevalent overexpression of COL11A1 in pan-cancer tissues, its profound prognostic and microenvironmental correlations, and the mechanistic underpinnings of its tumor-promoting effects as mediated via EMT signaling. Our findings suggest that COL11A1 could serve as a prognostic and diagnostic biomarker and therapeutic target for cancer.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"290"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Methanolic extract of wheatgrass (<i>Triticum aestivum</i> L.) prevents BPA-induced disruptions in the ovarian steroidogenic pathway and alleviates uterine inflammation in Wistar rats.","authors":"Ananya Barman, Angshita Ghosh, Tarun Kumar Kar, Sandip Chattopdhyay","doi":"10.1007/s13205-024-04117-0","DOIUrl":"10.1007/s13205-024-04117-0","url":null,"abstract":"<p><p>The present study examined the anti-inflammatory and functional improvement of the uterus and ovary, respectively, in bisphenol-A (BPA)-fed adult Wistar rats following the ingestion of methanolic extract of wheatgrass (WG-ME). Four groups of rats were conditioned as vehicle-treated control, BPA-treated (100 mg/kg b.w.), BPA + WG-ME (100 mg BPA/kg b.w. + 200 mg WG-ME/kg b.w.), and WG-ME (200 mg/kg b.w.) groups. The LC-MS study confirmed the presence of numerous bioactive components in WG-ME. ELISA, PAGE, real-time PCR, and immunohistostaining were executed to test the efficacy of WG-ME against BPA. WG-ME was shown to induce significant weight gain of the uterus and ovaries as well as improve the estrous cycle and antioxidant status. WG-ME effectively suppressed the mRNA expression of TNF-α (tumor necrosis factor-alpha) and NF-κB (nuclear factor kappa-B). This extract also increased the expression of the antiapoptotic factor BCL2 (B-cell lymphoma 2) in the uterine tissue of rats administered BPA while impeding the abnormal expression of the tumor proteins p53, cylcin-D1, and BAX (BCL2-associated protein X). An enhanced steroidogenic event was supported by improved gonadotropins and reproductive hormone levels, feeble signaling of androgen receptors, and improved ovarian follicular growth with a distinct appearance of granulosa layer as well as better uterine histomorphology. The abundance of apigenin and catechin compounds in WG-ME may potentiate the above effects. The molecular interaction study predicted that apigenin inhibits TNF-α by interacting with its major site. Hence, WG-ME may exert its preventive efficacy in managing the functional imbalance of reproductive organs caused by BPA.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04117-0.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"310"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586330/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In silico study, synthesis and antimalarial evaluation of hybrid pyridine substituted pyrazole 1,3,5-triazine derivatives.","authors":"Biswajit Devanath, Bonita Chetia, Anshul Shakya, Surajit Kumar Ghosh, Saurav Jyoti Patgiri, Ipsita Pal Bhowmick, Udaya Pratap Singh, Hans Raj Bhat","doi":"10.1007/s13205-024-04129-w","DOIUrl":"10.1007/s13205-024-04129-w","url":null,"abstract":"<p><p>Malaria is a significant global public health issue, particularly prevalent in Africa, Asia, and Latin America, necessitating urgent research into novel and efficient therapies. In the current research, we have designed pyridine substituted pyrazole 1,3,5-triazine derivatives as antimalarials. A library including 300 compounds, designated as <b>7S</b> (<b>1</b>-<b>300</b>), has been generated using a variety of aliphatic and aromatic amines. Ten compounds have been selected via in silico screening such as molecular properties, toxicity study, docking study and conventional synthesis for antimalarial evaluation against <i>P. falciparum</i> strains 3D7 (chloroquine-sensitive) and Dd2 (chloroquine-resistant). The docking results of compounds <b>7s258</b> and <b>7s5</b> revealed higher binding interaction with amino acids Leu46, Phe58, Phe116, Ala16 (-341.33 kcal/mol), Ser111, Ile112, Val45 Pro113, Leu119 (-335.16 kcal/mol) and Phe58, Ser111, Ile112, Phe116 (-354.47 kcal/mol), Phe58, Met55, Leu46, Leu164, Pro113 (-346.34 kcal/mol) against wild (1J3I) and quadruple mutant (1J3K) type of <i>Pf</i>-DHFR inhibitors. Further these compounds were synthesized by simple nucleophilic substitution reaction and characterized by different spectroscopic methods. The in vitro antimalarial assay results suggested that these compounds exhibit considerable antimalarial activity with IC<sub>50</sub> values of 32.74-46.80 μM and 28.05-54.95 μM against both the chloroquine-sensitive (3D7) and chloroquine-resistant (Dd2) strains of <i>P. falciparum</i>, respectively. Among the ten derivatives, compound <b>7s258</b> and <b>7s5</b> show substantial potential as antimalarial agents. They are highly suitable for further refinement in the field of drug development to effectively decrease the global malarial burden.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04129-w.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"301"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11568084/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
3 BiotechPub Date : 2024-12-01Epub Date: 2024-11-25DOI: 10.1007/s13205-024-04159-4
Noor Saba Khan, Saumya Choudhary, Mohd Ali, Mohd Shawaz, Benedikt Jakob Lohnes, Nitesh Kumar Poddar
{"title":"Unveiling biomarker detection in Alzheimer's disease: a computational approach to microarray analysis.","authors":"Noor Saba Khan, Saumya Choudhary, Mohd Ali, Mohd Shawaz, Benedikt Jakob Lohnes, Nitesh Kumar Poddar","doi":"10.1007/s13205-024-04159-4","DOIUrl":"https://doi.org/10.1007/s13205-024-04159-4","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a major neurodegenerative condition that affects a significant number of people around the world, making understanding the underlying molecular mechanisms fundamental for identifying predictive biomarkers and therapeutic targets for treating AD. Analysis of the gene expression profile GSE5281, consisting of 161 samples (87 AD and 74 control samples) revealed differentially expressed genes (DEGs) used for KEGG screening to connect dysregulated genes to metabolic pathways or other neurological diseases including Parkinson's, prion, and Huntington's and construction of a protein interaction network. Protein-protein interaction (PPI) network and module analysis uncovered the hub genes ACTB, ACTG1, ATP5A1, CCT2, CDC42, EGFR, FN1, GAPDH, GFAP, GRIA1, HSP90AB1, MAPK1, PSMA3, PSMD14, SNAP25, SNCA, SOD1, SOX2, TPI1, and YWHAZ. The analysis revealed a link between dysregulated genes and processes in AD pathology, including the promotion of osteoporosis, an altered nucleotide metabolism, microtubule stability, and the dysfunctionality of the blood-brain barrier (BBB). These targets might be used as predictive biomarkers or to develop curative and preventive therapeutic approaches for treating AD.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"311"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11589038/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142738010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Rational design of lanosterol 14α-demethylase for ergosterol biosynthesis in <i>Saccharomyces cerevisiae</i>.","authors":"Ruixue Liang, Kangjie Xu, Xinglong Wang, Wenqian Wei, Qihang Chen, Zhijie Qin, Weizhu Zeng, Jingwen Zhou","doi":"10.1007/s13205-024-04136-x","DOIUrl":"10.1007/s13205-024-04136-x","url":null,"abstract":"<p><p>Ergosterol is widely used in skin care products and drug preparation. Lanosterol 14α-demethylase (Erg11p, 14DM, CYP51) is the rate-limiting enzyme for the biosynthesis of various steroid compounds in <i>Saccharomyces cerevisiae</i>. Herein, Erg11p was engineered to extend the in vivo catalytic half-life and increase the turnover rate. Single mutations resulting in lower folding energy were selected, and mutant P201H had an ergosterol yield of 576.9 mg·L<sup>-1</sup>. Through consensus design, single mutations resulting in higher sequence identity to homologs were tested and mutant K352L had an ergosterol yield of 677.9 mg·L<sup>-1</sup>. The key residues for substrate binding were confirmed via alanine scanning mutagenesis and mutant F384A had an ergosterol yield of 657.8 mg·L<sup>-1</sup>. Molecular dynamics (MD) simulation was conducted to investigate the contributions of pocket residues and eight residues were found to engage in weak interactions with lanosterol. Saturation mutagenesis was applied to these residues to enhance binding to lanosterol, and mutant F384E had an ergosterol yield of 733.8 mg·L<sup>-1</sup>. Meanwhile, MD simulations were conducted to assess the impact of mutant F384E on enzyme activity. The results consistently showed that single point mutation F384E had the greatest effect, outperforming the combination mutations. Batch fermentation increased the ergosterol yield of mutant F384E to 3067.5 mg·L<sup>-1</sup>, the highest reported to date. The successful engineering of Erg11p may pave the way for industrial-scale production of ergosterol and other steroids.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04136-x.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"300"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11564469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
3 BiotechPub Date : 2024-12-01Epub Date: 2024-11-19DOI: 10.1007/s13205-024-04156-7
Jingya Li, Ziao Liu, Min Pan, Li Li, Xiaohui Tong, Yajuan Wang, Bin Chen, Tongsheng Wang
{"title":"Exploring the mechanism of carbamazepine decreasing testosterone levels based on cAMP/PKA/CREB pathway.","authors":"Jingya Li, Ziao Liu, Min Pan, Li Li, Xiaohui Tong, Yajuan Wang, Bin Chen, Tongsheng Wang","doi":"10.1007/s13205-024-04156-7","DOIUrl":"10.1007/s13205-024-04156-7","url":null,"abstract":"<p><p>The aim of this study was to explore the molecular mechanisms underlying carbamazepine (CBZ)-induced testicular toxicity and testosterone reduction in rats. For this purpose, Sprague-Dawley (SD) rats were intervened with 200 mg/kg CBZ for 12 weeks, and R2C cells were exposed to CBZ at concentrations of 0.5, 1 and 1.5 mM for 24 h. HE, Tunel, ELISA, immunofluorescence staining, RT-qPCR, and western blot were used to reveal the effects of CBZ on spermatozoa quality, testicular tissue structure, testosterone level and testosterone synthesis-related enzymes in rats. The results showed that CBZ significantly damaged the testicular tissue structure of rats, induced cell apoptosis, down-regulated the gene and protein expression levels of testosterone synthesis-related enzymes (STAR, TSPO, 17β-HSD and 3β-HSD), inhibited the expression of related proteins in the cAMP/PKA/CREB signalling pathway, and suppressed testosterone levels. In addition, the use of Db-cAMP (a PKA activator) significantly upregulated the protein expressions of PKA and p-CREB, evidently alleviated the CBZ-induced decrease in testosterone levels. In conclusion, CBZ induced testosterone resynthesis by inhibiting the cAMP/PKA/CREB pathway, affecting the expression of steroid synthesis-related enzymes and reducing testosterone levels.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"305"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11576689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"UPLC-QToF-MS/MS screening and characterization of <i>Symphorema polyandrum</i> Wight and in vitro assessment of its antioxidant, anticancer, and anti-inflammatory potential.","authors":"Dibya Ranjan Sahoo, Swaraj Kumar Babu, Baishali Basundhara Naik, Sajna Sameekshya Hota, Namita Bhoi, Barun Sarkar, S K Mustaq Ali, Pradeep Kumar Naik","doi":"10.1007/s13205-024-04144-x","DOIUrl":"10.1007/s13205-024-04144-x","url":null,"abstract":"<p><p><i>Symphorema polyandrum</i> belongs to the Lamiaceae family and is locally known as Badichang or mahasindhu. In this study, we performed Soxhlet extraction to prepare methanolic and hydromethanolic extracts, followed by quantification of their total phenolic content and total flavonoid content. Qualitative analysis of both the extracts was conducted to determine the presence of different phytochemicals. In addition, we aimed to identify the important phytochemical constituents in the methanolic extracts of <i>S. polyandrum</i> (SPM) using ultra-performance liquid chromatography hyphenated with high-resolution mass spectrometry (UPLC-ESI-QTOF-MS<sup>E</sup>). Furthermore, this study investigated the antioxidant, anticancer and anti-inflammatory properties of SPM and its safety profile in the normal fibroblast cell line L929. A colony proliferation assay and a Griess assay were performed to evaluate the effects of SPM on colony formation and nitric oxide (NO) production. A total of 13 important phytochemicals were identified and reported. The methanolic extract of SPM demonstrated significant antioxidant activity. SPM also showed substantial antiproliferative activity on MDA-MB-231 triple-negative breast cancer cells, with an IC<sub>50</sub> value of 45.53 ± 1.63 µg/ml, and also reduced the survival of these cancer cells by promoting nuclear fragmentation and condensation without causing harm to normal cells. SPM inhibits the colony formation and reduces the nitric oxide (NO) production. The anti-inflammatory potential of SPM was assessed utilizing the murine alveolar macrophages (J774.A.1) as an in vitro model, and SPM effectively lowered the levels of proinflammatory cytokines such as TNF-α and IL-6. These findings emphasized the antiproliferative potential of SPM to cancer cells, along with its anti-inflammatory, and antioxidant capabilities, indicating the therapeutic efficacy of this medicinal plant.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"298"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}