3 Biotech最新文献

{"title":"Virtual screening and molecular dynamics simulations of phytochemicals targeting cofactor-independent phosphoglycerate mutase in antimicrobial-resistant Mycoplasma genitalium","authors":"Krishnendu Barik, Pranabesh Mandal, Praffulla Kumar Arya, Durg Vijay Singh, Anil Kumar","doi":"10.1007/s13205-024-04082-8","DOIUrl":"https://doi.org/10.1007/s13205-024-04082-8","url":null,"abstract":"<p><i>Mycoplasma genitalium (M. genitalium)</i> poses a significant challenge in clinical treatment due to its increasing antimicrobial resistance. This study investigates alternative therapeutic approaches by targeting the cofactor-independent phosphoglycerate mutase (iPGM) enzyme with phytochemicals derived from ethnobotanical plants. In silico screening identified several promising inhibitors, with 2-carboxy-D-arabinitol demonstrating the highest binding affinity (− 9.77 kcal/mol), followed by gluconic acid (− 9.03 kcal/mol) and citric acid (− 8.68 kcal/mol). Further analysis through molecular dynamics (MD) simulations revealed insights into the binding mechanisms and stability of these phytochemicals within the iPGM active site. The MD simulations indicated initial fluctuations followed by stability, with intermittent spikes in RMSD values. The lowest RMSF values confirmed the stability of the ligand–protein complexes. Key residues, including Ser-61, Arg-188, Glu-62, Asp-397, and Arg-260, were found to play crucial roles in the binding and retention of inhibitors within the active pocket. These findings suggest that the identified phytochemicals could serve as novel antimicrobial agents against <i>M. genitalium</i> by effectively inhibiting iPGM activity.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of rice rhizosphere-isolated bacteria for their ability to stimulate plant growth and their antagonistic effects against Xanthomonas arboricola pv. juglandis","authors":"Cumhur Avsar","doi":"10.1007/s13205-024-04077-5","DOIUrl":"https://doi.org/10.1007/s13205-024-04077-5","url":null,"abstract":"<p>This study looked at the possibility of using bacteria that were separated from the rhizosphere of rice plants to promote plant development and offer biological control against pests that affect agriculture. A total of 119 bacteria were isolated from rice rhizospheres collected from six different locations. Of these, 15.47% showed phosphate solubilization, 47.05% showed IAA, 89.07% showed siderophore, and 10.08% showed ACC deaminase activity. Generally, high siderophore production was observed in strains showing ACC deaminase activity. The antagonistic behavior of all strains against the walnut pest <i>Xanthomonas arbiricola</i> was also studied, and eight (6.7%) isolates suppressed the growth of this pathogen (7–43 ± 2 mm zone diameter). It was also noted that these eight isolates showed almost exclusively siderophore activity. In contrast to IAA and siderophore synthesis, the study demonstrated reduced activity levels for phosphate solubilization and ACC deaminase. The 16S rRNA sequence results of some of the bacteria selected in this study and AFLP analysis based on some restriction enzymes showed that the diversity was quite high. According to the 16S rRNA analysis, the high antagonistic effect of strain 71, which is one of the members of the <i>Enterobacter</i> genus, shows that it can be used as a biocontrol agent. In this study, it was revealed in detail that bacteria can be preferred as alternative biological agents for plant growth instead of synthetic fertilizers. This is the first study on this subject in this region, which is one of the important points of the country in terms of rice production.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of cytotoxic potential of silver nanoparticles biosynthesized using essential oils of Jasminum sambac against breast cancer and bacterial cells","authors":"Ali Kadhum Bidan, Zainab Shakir Abdullah Al-Ali","doi":"10.1007/s13205-024-04058-8","DOIUrl":"https://doi.org/10.1007/s13205-024-04058-8","url":null,"abstract":"<p>Essential oils (EOs) which cover about 91% whole biomolecules formulated from <i>Jasminum sambac</i> leaves based on Gas chromatography-mass spectrometry were employed to identify structures. EOs were observed as good agents in the preparation of Silver nanoparticles (AgNPs) through the proposed mechanism that was attempted to interpret the pathway of the bio-preparation process. The characterization of EOs-AgNPs carried via ultraviolet–visible to reveal surface plasmon resonance at 420 nm, Fourier transform infrared to observe functional groups EOs compared to EOs-AgNPs. X-ray diffraction (XRD) revealed a broad chart owing to the small size of AgNPs in average size less than 10 nm calculated relying on image J software, spherical AgNPs with a small dispersive size observed by transmission electron microscopy. Quasi near spherical surface morphology of EOs-AgNPs had detected by field emission scanning electron microscope. EOs-AgNPs were assessed for their antibacterial potential against both Gram-positive (<i>Staphylococcus aureus</i>) and Gram-negative (<i>Escherichia coli</i>) bacteria as suppressing bacterial agents. EOs-AgNPs had their anti-breast cancer MCF-7 cell line ability investigated by DNA fragmentation; cycle flow cytometry (apoptosis) at half maximal inhibitory concentration (IC₅₀) was determined at 260 µg/mL which has been stated by cytotoxicity (MTT) assay. EOs-AgNPs have antibacterial and anticancer therapeutic potential, and it is safe, inexpensive, and scalable in the nanoscale range.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fusarium begoniae metabolites: a promising larvicidal, pupicidal potential, histopathological alterations and detoxifications enzyme profiles of medically important mosquito vector Aedes aegypti, Culex quinquefasciatus and Anopheles stephensi","authors":"Chinnasamy Ragavendran, Annadurai Govindaraj, Chinnaperumal Kamaraj, Devarajan Natarajan, Guilherme Malafaia, Abdulwahed Fahad Alrefaei, Mikhlid H. Almutairi","doi":"10.1007/s13205-024-04061-z","DOIUrl":"https://doi.org/10.1007/s13205-024-04061-z","url":null,"abstract":"<p>Endophytic fungal molecules have the potential to be a cost-effective chemical source for developing eco-friendly disease-controlling pharmaceuticals that target mosquito-borne illnesses. The primary aims of the study were to identify the fungus <i>Fusarium begoniae</i> larvicidal ability against <i>Aedes aegypti</i>, <i>Culex quinquefasciatus,</i> and <i>Anopheles stephensi.</i> The ethyl acetate extract demonstrated lethal concentrations that kill 50% of exposed larvae (LC₅₀) and 90% of exposed larvae (LC₉₀) for the 1st to 4th instar larvae of <i>An. stephensi</i> (LC₅₀ = 54.821, 66.525, 68.250, and 73.614; LC₉₀ = 104.56, 138.205, 150.415, and 159.466 μg/mL), <i>Cx. quinquefasciatus</i> (LC₅₀ = 64.981, 36.505, 42.230, and 36.514; LC₉₀ = 180.46, 157.105, 140.318, and 153.366 μg/ mL), and <i>Ae. aegypti</i> (LC₅₀ = 74.890, 33.607, 52.173, and 26.974; LC₉₀ = 202.56, 162.205, 130.518, and 163.286 μg/mL). Mycelium metabolites were evaluated for their pupicidal activity towards <i>Ae. aegypti</i> (LC₅₀ = 80.669, LC₉₀ = 119.904), <i>Cx. quinquefasciatus</i> (LC₅₀ = 70.569, LC₉₀ = 109.840), and <i>An. stephensi</i> (LC₅₀ = 73.269, LC₉₀ = 110.590 μg/mL). The highest larvicidal activity was recorded at 300 µg/mL, with 100% mortality against first and second-instar larvae of <i>Cx. quinquefasciatus</i>. Metabolite exposure to larvae exhibited several abnormal behavioral changes. The exposure to <i>F. begoniae</i> metabolite, key esterases such as acetylcholinesterase, α-and-β-carboxylesterase, and acid and alkaline phosphatase activity significantly decreased compared to control larvae. The outcomes of the histology analysis revealed that the mycelium metabolites-treated targeted larvae had a disorganized abdominal mid and hindgut epithelial cells. The is first-hand information on study of ethyl-acetate-derived metabolites from <i>F. begoniae</i> tested against larvae and pupae of <i>Ae. aegypti, Cx. quinquefasciatus</i> and <i>An. stephensi</i>. Bio-indicator toxicity findings demonstrate that <i>A. nauplii</i> displayed no mortality.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospects of crude enzymes in replacing pure enzymes for dissolving pulp production","authors":"Prabhjot Kaur, Jitender Sharma, Nishi Kant Bhardwaj, Shubhang Bhardwaj, Daljeet Kaur, Amarjit Singh, Ashish Kumar","doi":"10.1007/s13205-024-04080-w","DOIUrl":"https://doi.org/10.1007/s13205-024-04080-w","url":null,"abstract":"<p>High-purity cellulose from paper pulp can be obtained after appropriate treatments involving pure xylanases and cellulases/endoglucanases. This study investigated the efficacy of using crude xylanase and cellulase instead of commercial ones to improve process economics. Kraft paper grade pulp produced from veneer waste, hardwood, and non-wood sources was utilized as a more sustainable option. Crude xylanase and cellulase from isolated soil bacteria <i>Bacillus pumilus</i> 3GAH and <i>Bacillus subtilis</i> PJK6 were used for process optimization. The correlation between Fock reactivity, chain scission, and crystallinity after crude-cellulase treatment was established through chemical, FTIR, and XRD analyses. Pentosans in kraft pulp were reduced from an initial 18.7% to 4.9% through sequential treatments with crude xylanase and alkali. Subsequent crude-cellulase treatment, even at 8 U/g o.d. pulp, improved Fock reactivity from 28.2% to 61.2%, fulfilling a major criterion for viscose. Thus, crude enzymes can be effectively used for the efficient and economical upgrading of paper pulp to dissolving pulp.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the defense response in tomato against <i>Sclerotium rolfsii</i> by <i>Trichoderma asperellum</i> strain A10 through gene expression analysis.","authors":"C Shanmugaraj, Deeba Kamil, R Parimalan, Praveen Kumar Singh, P R Shashank, M A Iquebal, Zakir Hussain, Amrita Das, Robin Gogoi, K Nishmitha","doi":"10.1007/s13205-024-04040-4","DOIUrl":"10.1007/s13205-024-04040-4","url":null,"abstract":"<p><p>Biological control agents are preferred over chemicals for managing plant diseases, with <i>Trichoderma</i> species being particularly effective against soil-borne pathogens. This study examines the use of a highly antagonistic strain, <i>Trichoderma asperellum</i> A10, and a virulent strain, <i>Sclerotium rolfsii</i> Sr38, identified and confirmed through ITS, β-tubulin (<i>T. asperellum</i>), TEF 1α, and RPB2 (<i>S. rolfsii</i>) sequences. In vitro and in planta experiments compared the antagonistic potential of A10 with other antagonistic fungi and fungicides against <i>S. rolfsii</i>. A10 achieved 94.66% inhibition of <i>S. rolfsii</i> in dual culture assays. In greenhouse trials with tomato variety Pusa Ruby, A10 showed significant pre- and post-inoculation effectiveness, with disease inhibition of 86.17 and 80.60%, respectively, outperforming <i>T. harzianum</i>, Propiconazole, and Carbendazim. Additionally, microbial priming with A10 was explored to enhance plant defense responses. Pre-treatment of tomato plants with <i>T. asperellum</i> A10 led to significant upregulation of several defense-related genes, including PR1, PR2, PR3, PR5, PR12, thioredoxin peroxidase, catalase, polyphenol oxidase, phenylalanine ammonia lyase, isochorismate synthase, laccase, prosystemin, multicystatin, WRKY31, MYC2, lipoxygenase A, lipoxygenase C, proteinase inhibitor I, proteinase inhibitor II, and ethylene response 1 associated with various signaling pathways such as salicylic acid (SA)-mediated and jasmonic acid/ethylene (JA/ET)-mediated responses. This upregulation was particularly evident at 48 h post-inoculation in A10-primed plants challenged with <i>S. rolfsii</i>, inducing resistance against collar rot disease. This study underscores the effectiveness of <i>T. asperellum</i> A10 in controlling collar rot and highlights its potential for inducing resistance in plants through microbial priming, providing valuable insights into sustainable disease management strategies.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04040-4.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11344752/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142071724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical profiling and antibacterial activities of <i>Ziziphora tenuior</i> root extracts: a molecular docking against VanA of vancomycin-resistant enterococci.","authors":"Asma Hatami","doi":"10.1007/s13205-024-04056-w","DOIUrl":"10.1007/s13205-024-04056-w","url":null,"abstract":"<p><p>Medicinal plants, renowned for their antibacterial phytocompounds and secondary metabolites, hold significant promise in addressing antibiotic-resistant bacterial strains. This study aimed to conduct phytochemical profiling of the methanolic and dichloromethane extracts of <i>Ziziphora tenuior</i> root using the GC-MS technique. These extracts' antioxidant potential was assessed via DPPH assay and their antibacterial activity was evaluated against <i>S. aureus</i>, <i>E. coli</i>, and VRE bacterial strains. Furthermore, the drug-ligand interactions between the extracts' biocompounds and d-alanyl-d-lactate ligase (VanA) protein of vancomycin-resistant enterococci strains (VRE) were analyzed using molecular docking. Based on the results, 74% of methanolic extract consisted of (3methyl, 24S)-stigmast-5-en-3-ol (which is a β-sitosterol), followed by Tetrasiloxane, decamethyl (15.5%), and 1-methyl-4-phenyl-5-thioxo-1,2,4-triazolidin-3-one (10.5%). Also, the only predominant compound identified in the dichloromethane extract was Benzo[h]quinoline, 2,4-dimethyl-. Both extracts showed antioxidant activity, while the antioxidant activity of the methanolic extract (IC₅₀ = 95.33 μg/ml) was significantly higher than that of the dichloromethane extract (IC₅₀ = 934.23 μg/ml). Also, both extracts displayed substantial antibacterial efficacy against the tested pathogens, particularly against VRE. Moreover, the in silico analysis revealed that (3methyl, 24S)-stigmast-5-en-3-ol and Benzo[h]quinoline,2,4-dimethyl- exhibited notable interactions with VanA through docking energy values of - 9.0 and - 9.1 kcal/mol, respectively. Furthermore, these compounds formed 2 and 1 hydrogen bonds with VanA, respectively, highlighting their potential as effective interactants. These findings provide valuable visions into the therapeutic potentials of these plant-derived biocompounds in combating antibiotic-resistant bacterial infections.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11362404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142103306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancement of the degradation capacity of <i>Is</i>PETase by acidic amino acids insertion and carbohydrate-binding module fusion.","authors":"Chuang Li, Qingqing Zheng, Wei Liu, Quanyu Zhao, Ling Jiang","doi":"10.1007/s13205-024-04041-3","DOIUrl":"10.1007/s13205-024-04041-3","url":null,"abstract":"<p><p>The biocatalytic degradation of poly(ethylene terephthalate) (PET) through enzymatic methods has garnered considerable attention due to its environmentally friendly and non-polluting nature, as well as its high specificity. While previous efforts in enhancing <i>Is</i>PETase performance have focused on amino acid substitutions in protein engineering, we introduced an amino acid insertion strategy in this work. By inserting a negatively charged acidic amino acid, Glu, at the right-angle bend of <i>Is</i>PETase, the binding capability between the enzyme's active pocket and PET was improved. The resulted mutant <i>Is</i>PETase^9394insE exhibited enhanced hydrolytic activity towards PET at various temperatures ranging from 30 to 45 ℃ compared with the wild-type <i>Is</i>PETase. Notably, a 10.04-fold increase was observed at 45 ℃. To further enhance PET hydrolysis, different carbohydrate-binding modules (CBMs) were incorporated at the C-terminus of <i>Is</i>PETase^9394insE. Among these, the fusion of CBM from <i>Verrucosispora sioxanthis</i> exhibited the highest enhancement, resulting in a 1.82-fold increase in PET hydrolytic activity at 37 ℃ compared with the <i>Is</i>PETase^9394insE. Finally, the engineered variant was successfully employed for the degradation of polyester filter cloth, demonstrating its promising hydrolytic capacity. In conclusion, this research presents an alternative enzyme engineering strategy for modifying PETases and enriches the pool of potential candidates for industrial PET degradation.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11306670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141915855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification of <i>HSP90</i> gene family in <i>Rosa chinensis</i> and its response to salt and drought stresses.","authors":"Jun Xu, Shuangwei Liu, Yueming Ren, Yang You, Zhifang Wang, Yongqiang Zhang, Xinjie Zhu, Ping Hu","doi":"10.1007/s13205-024-04052-0","DOIUrl":"10.1007/s13205-024-04052-0","url":null,"abstract":"<p><p>Heat shock protein 90 (HSP90) is important for many organisms, including plants. Based on the whole genome information, the gene number, gene structure, evolutionary relationship, protein structure, and active site of the HSP90 gene family in <i>Rosa chinensis</i> and <i>Rubus idaeus</i> were determined, and the expression of the <i>HSP90</i> gene under salt, and drought stresses in two rose varieties Wangxifeng and Sweet Avalanche were analyzed. Six and eight <i>HSP90</i> genes were identified from <i>R. chinensis</i> and <i>Ru. idaeus</i>, respectively. Phylogenetic analysis revealed that the analyzed genes were divided into two Groups and four subgroups (Classes 1a, 1b, 2a, and 2b). Although members within the same classes displayed highly similar gene structures, while the gene structures and conserved domains of Group 1 (Class 1a and 1b) and the Group 2 (Class 2a and 2b) are different. Tandem and segmental duplication genes were found in <i>Ru. idaeus</i>, but not in <i>R. chinensis</i>, perhaps explaining the difference in <i>HSP90</i> gene quantity in the two analyzed species. Analysis of cis-acting elements revealed abundant abiotic stress, photolight-response, and hormone-response elements in <i>R. chinensis</i> HSP90s. qRT-PCR analysis suggested that <i>RcHSP90-1-1</i>, <i>RcHSP90-5-1</i> and <i>RcHSP90-6-1</i> in Sweet Avalanche and Wangxifeng varieties played important regulatory roles under salt and drought stress. The analysis of protein structure and active sites indicate that the potential different roles of <i>RcHSP90-1-1</i>, <i>RcHSP90-5-1</i>, and <i>RcHSP90-6-1</i> in salt and drought stresses may come from the differences of corresponding protein structures and activation sites. These data will provide information for the breeding of rose varieties with high stress resistance.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04052-0.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11330952/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142003327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in glycosyltransferase-mediated glycodiversification of small molecules.","authors":"Nguyen Huy Thuan, Quach Thi Thu Huong, Bui Dinh Lam, Ho Thanh Tam, Pham The Thu, Nguyen Xuan Canh, Vinay Bharadwaj Tatipamula","doi":"10.1007/s13205-024-04044-0","DOIUrl":"10.1007/s13205-024-04044-0","url":null,"abstract":"<p><p>Currently, numerous glycosides have been synthesized and used in clinical applications, neutraceuticals, cosmetics, and food processing. Structurally, a glycoside is composed of aglycone attaching to one or several sugar moieties so-called glycone. It is found that biochemical or biopharmaceutical properties of glycoside are mainly determined by its sugar part and thereby alternation of this glycone resulting in novel structure and characteristics as well. The use of traditional production methods of glycosides such as direct extraction and purification from plants, animals, or microorganisms is very challenging (laborious, time-consuming, technique, high price, low yield, etc.). Alternatively, the use of enzymatic methods for the biosynthesis of glycosides has become a highly promising tool. Particularly, the diverse structure of glycosides can be obtained using the promiscuous catalytic activity of glycosyltransferases (GT) mined from bioresources (plants, fungi, microorganisms, etc.). In addition, the exploration of GT catalytic promiscuity toward diverse aglycones, and glycones has indeed been interesting and played a key role in the production of novel glycosides. This review described the recent advances in glycosyltransferase-mediated glycodiversification of small molecules (flavonoids, steroids, terpenoids, etc.). Mostly, references were collected from 2014 to 2023.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11343957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142054629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}