3 Biotech最新文献

{"title":"CRISPR/Cas-mediated genome editing: playing a versatile role in mitigating the challenges of sustainable rice improvement.","authors":"Byomkesh Dash, Sudhansu Sekhar Bhuyan, Raj Kishore Sahoo, Nibedita Swain, Kishor Pundlik Jeughale, Suman Sarkar, Ram Lakhan Verma, C Parameswaran, B N Devanna, Sanghamitra Samantaray","doi":"10.1007/s13205-025-04494-0","DOIUrl":"10.1007/s13205-025-04494-0","url":null,"abstract":"<p><p>Just as Gregor Mendel's laws of inheritance laid the foundation for modern genetics, the emergence of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas systems has catalyzed a new era in precision genome engineering. CRISPR/Cas has revolutionized rice (<i>Oryza sativa</i> L.) breeding by enabling precise, transgene-free edits to improve yield, nutrition, and stress tolerance. Advanced tools like base and prime editing further refine these capabilities, offering powerful solutions for climate-resilient agriculture and global food security. The review synthesizes the CRISPR-mediated strategies for improving resistance against major biotic (bacterial blight, blast, sheath blight) and abiotic (drought, salinity, submergence, nutrient deficiency) stresses. Additionally, we explore the critical prerequisites for efficient genome editing in rice, ranging from target site design, PAM specificity, delivery systems (like <i>Agrobacterium</i>, RNPs, and nanoparticle-mediated delivery), to screening and validation of mutants. This review also highlights recent breakthroughs in multiplex genome editing for complex traits, including the development of haploid inducer lines and clonal seed technology. Haploid inducers accelerate breeding by producing homozygous lines without tissue culture, while engineered apomixis enables clonal propagation of elite hybrids. Beyond technical dimensions, this review underscores the broader socio-economic and regulatory implications of genome-edited rice, addressing the emerging ethical concerns, intellectual property issues, farmer access, and equitable technology dissemination in resource-limited agricultural regions. As the global policy landscape transitions to accommodate CRISPR-edited crops, transparent regulatory frameworks, stakeholder engagement, and public perception will play pivotal roles in ensuring sustainable, safe, and inclusive adoption of genome editing in agriculture.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"327"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12408448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated production and protein-protein docking analysis of <i>L</i>-methionase from <i>Klebsiella oxytoca</i> for enzyme-based anticancer therapy via methionine starvation and epigenetic modulation.","authors":"Bhupender Sharma, Vivek Chauhan, Vivek Kumar Dhiman, Rakesh Kumar, Gaytri Mahajan, Sukhdev Singh, Kanta Sashi, Anjali Kashwal, Shamsher S Kanwar","doi":"10.1007/s13205-025-04501-4","DOIUrl":"10.1007/s13205-025-04501-4","url":null,"abstract":"<p><p>Intracellular L-methionine γ-lyase (MGL) from <i>Klebsiella oxytoca</i> BLM-1 was produced and optimized using a combination of One-Factor-at-a-Time and Response Surface Methodology. Optimal culture conditions, such as pH 9.0, 3% (w/v) lactose, and 1.02% (w/v) malt extract, resulted in the highest intracellular MGL activity (0.235 U/mL), representing a 1.13-fold improvement over initial conditions, with a total yield of 40.80 U from a 2 L optimized broth. Purification using Octyl-Sepharose chromatography produced a highly active multimeric enzyme (~ 250 kDa) with 0.384 U/mL activity, which was confirmed as a heteromeric complex (~ 63 kDa and ~ 117 kDa subunits) by SDS-PAGE. The enzyme displayed strong cytotoxic activity toward methionine-dependent cancer cell lines, with IC₅₀ values of 0.023 U for HepG2 and 0.0045 U for A549, while exerting minimal effects on HEK-293 cells. Molecular docking revealed that L-methionine binds to the MGL active site with a binding energy of - 6.5 kcal/mol, and protein-protein docking identified favorable interactions with key methionine pathway enzymes, including METAP2 (- 902.3), MAT2A (- 895.1), and SAHH (- 890.9). These findings highlight the successful optimization of MGL production, its effective purification, and its significant anticancer potential, providing a strong foundation for its development as a therapeutic enzyme for methionine-dependent tumors.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04501-4.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"332"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12417354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multicomponent nanofibrous electrospun dressings, evaluated for the healing of full-thickness excision wounds in a porcine model.","authors":"Sobha Kota, Ratnakumari Anantha, Radhika Sajja, Pradeep Dumpala","doi":"10.1007/s13205-025-04460-w","DOIUrl":"10.1007/s13205-025-04460-w","url":null,"abstract":"<p><p>The first layer of the advanced wound dressing comprises a 3 mm layer of copper (oxide) nanoparticles synthesized with the leaf extracts of <i>M.</i> <i>oleifera</i> and <i>Musa</i>, mixed with <i>Cochlospermum</i> <i>gossypium</i> and <i>Acacia</i> <i>arabica</i> gums, and coated on the cotton fabric. By electrospinning at 20 kV, a 9-layered nanofibrous framework of the biopolymers cellulose acetate, gelatin, and marine chitosan, impregnated with bioactives such as carbon dots derived from medicinal plants, green synthesized copper nanoparticles, and phytochemicals extracted with acetone and isopropanol was woven. The core, shell and active layer nanofibers of different polymers, with a fiber diameter ranging from 252 nm to 3.1 µm, were grafted onto the CuNP-coated cotton fabric. Four wound dressings viz., WD1 (L)/WD2 (H) with poly l-lysine in low and high concentrations, and WD3 (L)/WD4 (H) with polyethylene oxide in low and high concentrations, were fabricated to improve the piezoelectric behavior. Of them, WD2 and WD4 showed promising biological characteristics of anti-inflammatory, anti-oxidant, and anti-microbial potential. They were endowed with piezoelectric constants of 24.28 pm/V (WD2) and 0.28 nm/V (WD4), and zeta potentials of 57.7 mV (WD2) and 94.6 mV (WD4). Within 8 days of causing full-thickness wounds, the two test items were able to effectively cause quick wound contraction, epithelial tissue regrowth, and elimination of inflammation; by 34 days, normalcy had returned.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04460-w.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"335"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425873/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, partial characterization of nanochitosan from cuttlebone waste of <i>Sepia prashadi</i> and its in vitro anticancer potentials.","authors":"Vipra Sharma, Annathai Pitchai, Revathi Duraisamy, Dhanraj Ganapathy, Pasiyappazham Ramasamy","doi":"10.1007/s13205-025-04504-1","DOIUrl":"https://doi.org/10.1007/s13205-025-04504-1","url":null,"abstract":"<p><p>Cuttlebone waste, a marine byproduct, was used as a sustainable source for the extraction of chitosan and synthesis of nanochitosan. This study looks into how to make nanochitosan from the cuttlebone waste of <i>Sepia prashadi</i> and some of its properties. It shows that this material could be used in environmentally friendly ways. Nanochitosan was prepared using a sustainable extraction method followed by ionic gelation. Physicochemical properties were analyzed using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM), revealing its nanostructured morphology and functional group integrity. The cytotoxicity of nano chitosan was evaluated in vitro against human oral cancer cell lines (KB) using MTT assay. Nanochitosan typically exhibits characteristic FTIR peaks, including an amide II peak with N-H stretching at 2880 cm⁻¹ and C-H stretching at 1531 cm⁻¹, confirming its functional groups. SEM studies show that nanochitosan particles range from 70 to 135 nm in size, characterized by their small, uniform morphology, contributing to their unique biological and chemical properties. Nanochitosan exhibited significant dose-dependent cytotoxicity, indicating its potential as an anticancer agent<b>.</b> This study demonstrates both the eco-friendly synthesis and the promising anticancer potential of nanochitosan derived from marine biowaste.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"342"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Curcumin improves diabetic vascular aging rats and high glucose-induced cells aging by promoting mitophagy.","authors":"Jinlin Wu, Xi Mei, Yong Li, Fang Liu, Dongfang Liu","doi":"10.1007/s13205-025-04510-3","DOIUrl":"10.1007/s13205-025-04510-3","url":null,"abstract":"<p><p>Vascular aging was reported to be closely related to diabetes. This study investigates the inhibitory effects and mechanism of curcumin on diabetic vascular aging by regulating mitophagy through the PINK1 pathway. The diabetic rat model was established by feeding with a high-fat diet and intraperitoneal injection of streptozotocin (STZ), and treated with high-dose (200 mg/kg), low-dose (50 mg/kg) curcumin, or metformin (200 mg/kg), respectively. The role of mitophagy in high glucose (HG)-induced human aortic smooth muscle cells (HASMCs) aging in vitro were investigated. The results indicated that curcumin ameliorated weight loss and improved elevated blood glucose levels in diabetic rats. Curcumin also improved the vascular pathological changes of the common carotid artery, decreased the vascular interstitial collagen fiber and vascular calcium salt deposition, and improved vascular ultrastructure. Furthermore, curcumin significantly decreased ET-1, VCAM-1, and p16 expressions. In addition, curcumin increased the expression of LC3II/I, Beclin1, and PINK1 proteins, while decreasing p62 expression. High-dose curcumin could improve mitochondrial morphology and increase mitochondrial autophagy. Additionally, curcumin increased HASMCs viability and inhibited HG-induced vascular aging by promoting mitophagy in vitro. WB confirmed that LC3II/I, Beclin1, and PINK1 levels were increased, while p62 and p16 levels were decreased. The improvement effect of curcumin on vascular aging was reversed by the mitophagy inhibitor Mdivi-1 or PINK1 siRNA. In conclusion, curcumin alleviates vascular aging in diabetic rats and HG-induced senescence in HASMCs by enhancing mitochondrial autophagy. These results suggest that curcumin has therapeutic potential in alleviating diabetic vascular aging.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04510-3.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"341"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12431978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient bioconversion of deinked paper sludge into valuable biobased succinic acid by engineered <i>Escherichia coli</i>.","authors":"Chotika Gosalawit, Walainud Congthai, Kanyarat Onsanoi, Chutchawan Phosriran, Kuan-Chen Cheng, Kata Buda, Csaba Feher, Kaemwich Jantama","doi":"10.1007/s13205-025-04522-z","DOIUrl":"https://doi.org/10.1007/s13205-025-04522-z","url":null,"abstract":"<p><p>With the ongoing rise in petroleum prices, there has been growing interest in the microbial production of succinic acid from industrial wastes. Deinked paper sludge (DPS) is a significant waste, rich in cellulose and hemicellulose, generated in paper recycling plants. The disposal of DPS raises environmental concerns, and potential health hazards. This study explored an alternative and integrated platform, combining effective pre-treatment and enzymatic hydrolysis of DPS, and efficient fermentation process, for biobased succinic acid production by <i>Escherichia coli</i> KJ122. Initially, 10% (w/v) DPS was pre-treated with 1 N NaOH for 16 h followed by autoclaving at 120 °C for 20 min to remove inhibitors. The cellulase loading of 60 PCU (Protein Centered Unit)/g NaOH-pre-treated DPS was found to be optimal to release the highest fermentable sugars to 84.6 ± 1.8% after hydrolysis. For separated hydrolysis and fermentation (SHF), succinic acid at 27.80 ± 0.37 g/L with a yield of 0.68 ± 0.20 g/g and productivity of 0.50 ± 0.00 g/L/h was achieved. However, DPS loading at 100 g/L NaOH-pre-treated DPS in SHF posed challenges due to high viscosity of the broth, requiring increased agitation and energy consumption, and prolonged operating time, particularly for large-scale operations. To address these issues, a fed-batch semi-SHF process was employed, significantly improving succinic acid yield (0.97 ± 0.01 g/g; 88.1% theoretical maximum) and productivity (0.55 ± 0.02 g/L/h). According to the mass balance analysis, producing 1 kg of succinic acid required 2.5 kg NaOH-pre-treated DPS. This work provides an alternative and effective method for converting DPS into high-value bio-succinic acid, offering potential applications in the paper industry and contributing to bio-circular economy and zero-waste principles.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"348"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436256/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sesamin and α-linolenic acid protection against hyperlipidemia-induced obesity in <i>Drosophila melanogaster</i> model.","authors":"Abu Safana Biswas, Rajeshwari Selvaraj, Kamsagara Linganna Krishna, Ganavi Bethanagere Ramesha, Bharat Jayaprakash Byalahunashi, Seema Mehdi, Suman Pathak","doi":"10.1007/s13205-025-04532-x","DOIUrl":"https://doi.org/10.1007/s13205-025-04532-x","url":null,"abstract":"<p><p>This study evaluates the anti-hyperlipidemic activity of phytoconstituents, such as sesamin and α-linolenic acid (ALA), in a high-fat diet (HFD)- induced <i>Drosophila melanogaster</i> obese model. Male wild-type <i>Drosophila</i> were fed an HFD (10% coconut oil) to induce obesity for 5 days. The obese flies were treated with the test compounds (sesamin, ALA) and rosuvastatin as a standard drug. Sesamin and ALA were tested for acute toxicity studies to calculate the doses for the efficacy study. Body weight, climbing, negative geotaxis, and cold stress were performed as behavioural assays, and lipid profiling (total cholesterol, triglycerides (TG, LDL, and HDL), glucose levels, and total protein content was determined as biochemical assays. Results demonstrated that sesamin and ALA have good potency in significantly improving behavioural parameters (P < 0.05). However, when compared, ALA showed better potency in enhancing these parameters. ALA has better potency in reducing TG and LDL levels, which are responsible for cardiovascular disease. Sesamin has better efficacy in improving the level of HDL, which an HFD reduces in flies, which is responsible for atherosclerosis. Sesamin reduced lipid profile in a dose-dependent manner, but required higher doses to achieve comparable lipid-lowering effects. In conclusion, ALA exhibited more substantial protective effects in reducing hyperlipidemia compared to control flies as an anti-hyperlipidemic agent in a dose-dependent manner.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"363"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12480314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untargeted metabolomics reveals <i>Syzygium cumini</i> (L.) alleviates ER stress and redox damage via activation of ATF-6/CHOP/NF-κB signaling axis in diabetic rats.","authors":"Karan Singh Yadav, Gurvinder Singh, Anjali Mishra, Amol Chhatrapati Bisen, Akash Ranjan, Rabi Sankar Bhatta, Vineeta Tripathi, Dinesh Kumar, Madhav Nilakanth Mugale","doi":"10.1007/s13205-025-04523-y","DOIUrl":"https://doi.org/10.1007/s13205-025-04523-y","url":null,"abstract":"<p><p>This study reports the therapeutic potential of aqueous seed extract <i>Syzygium cumini</i> (AESC) in alleviating type 1 diabetes mellitus (T1DM) associated endoplasmic reticulum (ER) and oxidative stress in a streptozotocin (STZ)-induced rat model. The LC-MS/MS analysis of AESC revealed gallic acid, ellagic acid, quercetin, malic acid, and citric acid as major antidiabetic phytoconstituents. Administration of AESC (250 mg/kg) normalized fasting blood glucose levels to those of healthy control rats. Moreover, AESC increases antioxidant levels and downregulates ER and inflammatory markers. Histopathological evaluation showed improved pancreatic tissue architecture, and immunohistochemistry revealed enhanced insulin expression within the islets. Mechanistically, AESC alleviated ER stress and oxidative damage through the ATF-6/CHOP/NF-κB signaling axis. Furthermore, serum metabolomics indicated aberrant accumulation of branched-chain amino acids and reduced 3-hydroxybutyrate levels (increased ketolysis) in diabetic rats that were reversed by AESC. This study is limited by using a single dosage of AESC and short-term evaluation, emphasizing acute rather than long-term effects. Furthermore, the lack of in vitro validation and genetic knockdown approaches restricts confirmation of the specific mechanisms underlying the antidiabetic action of the extract. Future studies employing multiple dosage regimens, chronic treatment models, and molecular validation strategies are warranted to establish the long-term efficacy, safety, and mechanistic specificity of AESC as a potential therapeutic for T1DM.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04523-y.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"356"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12449296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophage marker gene-driven prognostic models for esophageal cancer: integrating multi-omics analysis and therapeutic strategies.","authors":"Zehan Li, Huazhen Wu, Chuzhong Wei, Yunton Jia, Weiqi Zhao, Xiaoli Feng, Fanghui Bian, Pingmei Zhang, Qiyu Liao, Zhiyong Pan, Lingxin Zeng, Jiemin Liang, Yuxiao Tian, Xinyu Wang, Yuyi Liu, Xin Wang, Song Zhu, Ruiming Tang","doi":"10.1007/s13205-025-04452-w","DOIUrl":"10.1007/s13205-025-04452-w","url":null,"abstract":"<p><p>By integrating single-cell and bulk RNA-sequencing data for esophageal cancer (ESCA), we developed and validated a seven-macrophage-gene prognostic signature (FCN1, SCARB2, ATF5, PHLDA2, GLIPR1, CHORDC1, and BCKDK). This signature effectively stratified patients into high- and low-risk groups with significantly different overall survival, achieving area under the curve (AUC) values greater than 0.7 for 1-, 2-, and 3-year survival prediction. A high-risk status correlated with an immunosuppressive tumor microenvironment, characterized by lower infiltration of B cells and CD8 + T cells, and was associated with reduced sensitivity to multiple chemotherapeutic agents, including Cisplatin and 5-Fluorouracil. Conversely, a low-risk status was linked to greater immune cell infiltration and higher predicted chemosensitivity. At the single-cell level, pseudotime analysis revealed that macrophage maturation significantly correlated with a decreasing risk score, suggesting that mature macrophages may contribute to a favorable prognosis. Furthermore, cell communication analysis identified high-risk macrophages as dominant drivers of a pro-tumorigenic microenvironment via signaling pathways, such as SPP1 and complement. In conclusion, this seven-gene signature is a robust prognostic biomarker that offers a new strategy for personalized risk assessment and treatment selection in ESCA.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04452-w.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"329"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411374/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling of the bacterial community and the degradative capability of newly isolated poly(lactic acid) (PLA)- and poly(butylene succinate) (PBS)-degrading bacteria from coastal samples.","authors":"Ausawadee Phonlamai, Wanthanee Khetkorn, Voranuch Thongpool, Titiporn Panyachanakul, Chanwit Suriyachadkun, Vichien Kitpreechavanich, Chatsuda Sakdapetsiri, Thanasak Lomthong","doi":"10.1007/s13205-025-04521-0","DOIUrl":"https://doi.org/10.1007/s13205-025-04521-0","url":null,"abstract":"<p><p>The coastal area of Thailand is a tropical marine environment with high microbial diversity, providing favorable conditions for microorganisms capable of degrading bioplastics. The current study aimed to investigate the bacterial community profiling of four samples collected from a coastal area in Thailand and to isolate the potential thermophilic bacteria with the ability to produce bioplastic-degrading enzymes. Our analysis revealed site-specific predominant genera: <i>Brevibacillus</i> in seawater (64.34 ± 0.27%), <i>Pseudomonas</i> in plastic waste (39.69 ± 3.77%), <i>Pseudoalteromonas</i> in soil (54.83 ± 2.40%), and <i>Psychrobacter</i> in moss rock (41.01 ± 1.67%). The thermophilic bacteria, including 6 poly(lactic acid) (PLA)- and 3 poly(butylene succinate) (PBS)-degrading bacteria, were isolated using a two-step technique in an emulsified polymer medium. These nine isolates were classified into five species across four genera: <i>Brevibacillus gelatini</i>, <i>Microbispora rosea</i>, <i>Actinomadura keratinilytica</i>, <i>Paenibacillus thermoaerophilus</i>, and <i>P. ginsengihumi</i>. Among these, <i>Actinomadura keratinilytica</i> LDF1 and <i>M. rosea</i> BS2-4 exhibited the highest enzymatic activities for PLA and PBS degradation (0.87 ± 0.11 U/mL and 0.31 ± 0.03 U/mL, respectively). Scanning electron microscopy confirmed the degradation capabilities of these strains in culture medium. Crude enzyme from the LDF1 strain demonstrated versatility in degrading various types of PLA, including PLA film, PLA powder, commercial cup, and commercial cutlery, while the strain BS2-4 enzyme effectively degraded PBS in film, powder, commercial cup, and commercial drinking straw. These findings advance our understanding of coastal microbial ecology and also highlight the potential of indigenous bacteria for bioplastic waste management, contributing to sustainable environmental solutions.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04521-0.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"352"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12443653/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}