{"title":"Efficient production of the high-intensity natural sweetener siamenoside I by the exo-1,3-beta glucanase (Exo15) from <i>Meyerozyma guilliermondii</i> LHGNSJ-VS01.","authors":"Hongjiang Wang, Haifeng Xie, Ailing Zhong, Qilin Xie","doi":"10.1007/s13205-025-04260-2","DOIUrl":"10.1007/s13205-025-04260-2","url":null,"abstract":"<p><p>The scarcity of siamenoside I (SI) hindered its widespread application. Addressing this challenge, we devised an innovative biocatalytic strategy and biological solution for large-scale SI production. Endo 15, an endophyte from <i>Siraitia grosvenorii</i>, exhibited excellent proficiency in SI synthesis, achieving a remarkable 50.65% SI abundance. By harnessing the extracellular protein of Endo 15 (EP), we further escalated SI abundance to 83.59 ± 2.5%, accompanied by full substrate conversion. Delving into the underlying mechanisms, we identified Exo15, a distinct functional protein derived from EP, displaying merely 48.88% amino acid similarity to the yeast exo-1,3-beta glucanase (Exg1). Successfully overexpressing Exo15 in <i>E. coli</i>, we confirmed its functionality in line with EP. Exo15 exhibited exceptional catalytic prowess, efficiently hydrolyzing mogroside V into the high-potency sweetener SI, with unparalleled activity and specificity. Our groundbreaking approach yielded an impressive SI titer of 54 g/L, coupled with an average conversion rate of 2.5 g/L per hour. These outstanding outcomes underscore the immense potential of Exo15 in cost-effective industrial production of the premium natural sweetener, siamenoside I, paving the way for its widespread adoption.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04260-2.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"94"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
3 BiotechPub Date : 2025-04-01Epub Date: 2025-03-07DOI: 10.1007/s13205-025-04252-2
Argajit Sarkar, Surajit Bhattacharjee
{"title":"Biofilm-mediated bioremediation of xenobiotics and heavy metals: a comprehensive review of microbial ecology, molecular mechanisms, and emerging biotechnological applications.","authors":"Argajit Sarkar, Surajit Bhattacharjee","doi":"10.1007/s13205-025-04252-2","DOIUrl":"10.1007/s13205-025-04252-2","url":null,"abstract":"<p><p>Environmental pollution, driven by rapid industrialization and urbanization, has emerged as a critical global challenge in the twenty-first century. This comprehensive review explores the potential of bacterial biofilms in bioremediation, focusing on their ability to degrade and transform a wide array of pollutants, including heavy metals, persistent organic pollutants (POPs), oil spills, pesticides, and emerging contaminants, such as pharmaceuticals and microplastics. The unique structural and functional characteristics of biofilms, including their extracellular polymeric substance (EPS) matrix, enhanced genetic exchange, and metabolic cooperation, contribute to their superior pollutant degradation capabilities compared to planktonic bacteria. Recent advancements in biofilm-mediated bioremediation include the application of genetically engineered microorganisms, nanoparticle-biofilm interactions, and innovative biofilm reactor designs. The CRISPR-Cas9 system has shown promise in enhancing the degradative capabilities of biofilm-forming bacteria while integrating nanoparticles with bacterial biofilms demonstrates significant improvements in pollutant degradation efficiency. As global pollution rises, biofilm-based bioremediation emerges as a cost-effective and environmentally friendly approach to address diverse contaminants. This review signifies the need for further research to optimize these techniques and harness their full potential in addressing pressing environmental challenges.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"78"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889332/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
3 BiotechPub Date : 2025-04-01Epub Date: 2025-03-23DOI: 10.1007/s13205-025-04233-5
Ahmed Z Ezz Eldeen, Fatma El-Zahraa A Abd El-Aziz, Ahmed M Sayed, Sayed A S Mousa, Mostafa A Asmaey
{"title":"Exploring the phytochemical composition of <i>Salsola imbricata</i>: investigating its protective potential against UV-C radiation in earthworms and isopods models.","authors":"Ahmed Z Ezz Eldeen, Fatma El-Zahraa A Abd El-Aziz, Ahmed M Sayed, Sayed A S Mousa, Mostafa A Asmaey","doi":"10.1007/s13205-025-04233-5","DOIUrl":"10.1007/s13205-025-04233-5","url":null,"abstract":"<p><p>The aqueous ethanolic extract of <i>Salsola imbricata</i> (AEESI) demonstrated significant protective effects against UV-C radiation damage, using earthworms and isopods as models for human skin and eyes, respectively. High-performance liquid chromatography (HPLC) analysis identified 15 bioactive polyphenolic compounds in AEESI, with chlorogenic acid (55.51 µg/ml) and gallic acid (46.69 µg/ml) as the dominant phenolic acids, and naringenin (40.42 µg/ml) as the primary flavonoid. The extract effectively mitigated histological and ultrastructural damage caused by UV-C radiation in both models. Additionally, quality control parameters, including moisture content, pH, acidity index, ash content, and elemental composition, were determined for the first time. These findings highlight the potential of <i>S. imbricata</i> extract as a protective agent against UV-C radiation-induced damage, attributed to its rich polyphenolic content.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"97"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11929650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143699330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Inhibitory effects of silver and copper oxide nanoparticles, synthesized using <i>Juglans regia</i> green husk aqueous extract, on human insulin fibrillation.","authors":"Setayesh Shevidi, Seyyed Abolghasem Ghadami, Parinaz Ghadam, Neda Arghand","doi":"10.1007/s13205-025-04257-x","DOIUrl":"10.1007/s13205-025-04257-x","url":null,"abstract":"<p><p>Recent research indicates that nanoparticles can serve as tools for the diagnosis and treatment of diseases. This study investigates the inhibitory effects of silver and copper oxide nanoparticles, synthesized using <i>Juglans regia</i> green husk aqueous extract, on human insulin fibrillation. Initially, the formation of amyloid fibrils in recombinant human insulin protein was examined under various buffers and by altering physicochemical conditions, such as pH and temperature, identifying optimal conditions for fibril formation. The nanoparticles were synthesized and characterized for size using dynamic light scattering (DLS), morphology via scanning electron microscopy (SEM), and surface charge through zeta potential analysis. Utilizing biochemical and biophysical techniques, including turbidimetry, DLS, SEM, and fluorescence spectroscopy, we demonstrate that both nanoparticle types effectively inhibit insulin fibrillation, with copper nanoparticles exhibiting superior efficacy. Bioinformatics analyses, combined with zeta potential measurements, suggest that the inhibitory effects of the nanoparticles arise from interactions with charged regions of the insulin molecule. These findings highlight the critical role of nanoparticle characteristics in modulating protein aggregation and present promising therapeutic potential for addressing amyloid-related diseases. Future research should aim to optimize nanoparticle design and evaluate their pharmacokinetics to improve their clinical applicability.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"98"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11930910/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143708059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of CRISRP/Cas9-based TP53-knockout pig muscle stem cells for use in the cultured meat industry.","authors":"Witsanu Srila, Amorn Pangjantuk, Phongsakorn Kunhorm, Nipha Chaicharoenaudomrung, Parinya Noisa","doi":"10.1007/s13205-025-04225-5","DOIUrl":"10.1007/s13205-025-04225-5","url":null,"abstract":"<p><p>Muscle satellite cells (MSCs) are essential for cultured meat production although their restricted lifespan and diminished stemness during prolonged culture pose significant limitations. This study established immortalized porcine MSCs using <i>TP53</i> gene deletion with the CRISPR/Cas9 technology. Several <i>TP53</i>-knockout (KO) clones were generated, exhibiting indel alterations and monoallelic and biallelic deletions. These clones exhibited markedly prolonged cellular lifespans compared to wild-type cells, overcoming the constraints of senescence. The growth rates and the proliferation marker (ki67) gene expression (passage 20) in the <i>TP53</i>-KO clones were dramatically elevated compared to the WT cells. All <i>TP53</i>-KO clones demonstrated a loss of stemness, proliferation, and muscle differentiation marker gene expression during long-term cell culture except for Desmin expression in the <i>TP53</i>-KO 42 clone. Immortalized <i>TP53</i>-KO clones maintained the ability to express muscle-specific protein markers compared to wild-type cells. Moreover, all clones had non-tumorigenic behavior, except <i>TP53</i>-KO clones 41 and 42, which displayed tumorigenic potential. <i>TP53</i>-KO clones demonstrated enhanced myogenic differentiation efficiency after multiple passages in comparison to wild-type cells. The results highlight the potential of <i>TP53</i>-KO MSCs as a cellular resource for future cultured meat production.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04225-5.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"92"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
3 BiotechPub Date : 2025-04-01Epub Date: 2025-03-05DOI: 10.1007/s13205-025-04249-x
Jing Wu, Sen Liu, QiQi Song, FuLin Tao, WenTao Zhu, FuPing Zhuang, Wei Fang, ZeGeng Li, DianLei Wang
{"title":"Triple regulation of oxidative-acetylation cycling pathways in COPD glucocorticoid resistance by HuaTanJiangQi capsules.","authors":"Jing Wu, Sen Liu, QiQi Song, FuLin Tao, WenTao Zhu, FuPing Zhuang, Wei Fang, ZeGeng Li, DianLei Wang","doi":"10.1007/s13205-025-04249-x","DOIUrl":"10.1007/s13205-025-04249-x","url":null,"abstract":"<p><p>Glucocorticoid (GC) resistance in chronic obstructive pulmonary disease (COPD) induced by long-term smoking, significantly reduces the quality of life of patients. The complex interaction between antioxidants and acetylation is an important factor that contributes to the slow progression of treatment. This study highlights the development of GC resistance in COPD through 4-hydroxynonenal (4-HNE), multidrug resistance-associated protein 1 (MRP1), histone deacetylase 2 (HDAC2), and nuclear related factor 2 (Nrf2), using enzyme-linked immunosorbent assays, western blotting, and siRNA silencing. Our results suggest that long-term exposure to cigarette smoke can increase 4-HNE toxicity via reactive oxygen species (ROS)-induced lipid peroxidation and decrease the expression of MRP1, histone HDAC2, and Nrf2. Together, these molecules form and enhance the cyclic resistance pathway in COPD, including MRP1 reducing 4-HNE efflux, 4-HNE down-regulating HDAC2 expression by oxidation, HDAC2 reducing Nrf2 transcription by deacetylation, and Nrf2 reducing MRP1 expression through acetylation. The HuaTanJiangQi Capsule (HTJQ) reduces GC resistance via a triple regulatory pathway by enhancing the activity of HDAC2, promoting the transcription of Nrf2, up-regulating the expression of MRP1, and reducing lipid peroxidation induced by ROS. Thus, this cyclic mechanism of GC resistance in COPD may open new avenues for robust therapies using HTJQ.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"72"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Beyond the genome: unveiling tissue-specific non-coding RNAs in clove (<i>Syzygium aromaticum L.</i>).","authors":"Nitesh Kumar Sharma, Dwijesh Chandra Mishra, Baibhav Kumar, Sudhir Srivastava, Krishna Kumar Chaturvedi, Awani Kumar Singh, Sharanbasappa D Madival, Neeraj Budhlakoti, Girish Kumar Jha","doi":"10.1007/s13205-025-04251-3","DOIUrl":"10.1007/s13205-025-04251-3","url":null,"abstract":"<p><p>Clove (<i>Syzygium aromaticum</i>), valued for its role in food preservation and medicine, has recently drawn research interest for its noncoding RNAs (ncRNAs). This study discovers 3274 long noncoding RNAs (lncRNAs) and 2404 circular RNAs (circRNAs) from publicly available RNAseq data. We identified the regulation of 834 genes through miRNA-lncRNA-mRNA network interactions. Additionally, 35 lncRNAs were predicted as precursors for 17 microRNAs (miRNAs), highlighting their role in post-transcriptional regulation. Tissue-specific analysis of circRNAs revealed their interaction with 1047 miRNAs and competing for binding sites on 2382 messenger RNAs (mRNAs). These results underscore their involvement in complex regulatory networks. To support further research and development, we developed SaroNcRDb (http://backlin.cabgrid.res.in/saroncrdb/), a web resource providing detailed insights into the types, chromosomal locations, tissue distributions, and interactions of identified ncRNAs. The findings pave the way for future studies to harness the regulatory roles of ncRNAs in improving Clove's agronomic traits and secondary metabolite production.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"81"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"POGZ targeted by LINC01355/miR-27b-3p retards thyroid cancer progression via interplaying with MAD2L2.","authors":"Jiancan Lu, Xinglu Zhou, Hongling Zhu, Mei Zou, Lianyong Liu, Xiangqi Li, Mingjun Gu","doi":"10.1007/s13205-025-04231-7","DOIUrl":"10.1007/s13205-025-04231-7","url":null,"abstract":"<p><p>Despite the high morbidity of thyroid cancer (THCA), the underlying molecular pathology remains elusive. That autism-associated protein POGZ has recently been involved in tumorigenesis intrigues us exploring its relevant molecular regulatory network in THCA. Clinical characteristics and intermolecular relationships were dissected by bioinformatics. Interaction between POGZ and MAD2L2 was examined by Co-IP assay. Targeting relationships between miR-27b-3p and POGZ/LINC01355 was verified by sequence prediction and dual-luciferase reporter detection. Cellular effects of genes were assessed by CCK-8 assay, clone formation assay, and Transwell assay, and further confirmed by a tumor-bearing nude mice model. Our results demonstrated a decrease in POGZ expression in THCA tissues and cell lines, and an interaction between POGZ and MAD2L2 protein. POGZ inhibited both the proliferation and motility of THCA cells, with these effects being reversed upon MAD2L2 silencing. LINC01355 exhibited low expression level and a positive correlation with POGZ in THCA. Both miR-27b-3p and LINC01355 were identified as regulators of POGZ through targeting. Elevated miR-27b-3p suppressed POGZ expression. LINC01355 promoted POGZ and counteracted the inhibitory effects of miR-27b-3p. Furthermore, miR-27b-3p increased the proliferation and motility of THCA cells, an effect that was blocked by LINC01355. At the animal level, POGZ, LINC01355, and MAD2L2 all attenuated tumor growth in THCA. Collectively, POGZ restrains THCA growth by interacting with MAD2L2 protein, and POGZ modulation involves a complex interplay orchestrated by LINC01355-targeted miR-27b-3p. By reporting the first POGZ-focused ceRNA network involving noncoding RNA in THCA, our study paves the way for exploring POGZ-related pathways and developing new therapeutic strategies in cancer.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04231-7.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"79"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
3 BiotechPub Date : 2025-04-01Epub Date: 2025-03-28DOI: 10.1007/s13205-025-04266-w
Himanshu Jangid, Gaurav Kumar
{"title":"Ecotoxicity of fungal-synthesized silver nanoparticles: mechanisms, impacts, and sustainable mitigation strategies.","authors":"Himanshu Jangid, Gaurav Kumar","doi":"10.1007/s13205-025-04266-w","DOIUrl":"10.1007/s13205-025-04266-w","url":null,"abstract":"<p><p>The review investigates the ecotoxicological implications of fungal-synthesized silver nanoparticles (AgNPs), focusing on their behavior, transformations, and impacts across aquatic and terrestrial ecosystems. Advanced techniques, such as Single-Particle ICP-MS and Nanoparticle Tracking Analysis, reveal the persistence and biotransformation of AgNPs, including silver ion (Ag⁺) release and reactive oxygen species (ROS) generation. The review highlights species-specific bio-accumulation pathways in algae, soil microbes, invertebrates, and vertebrates, along with the limited biomagnification potential within trophic levels. Long-term exposure to AgNPs leads to reduced soil fertility, altered microbial communities, and inhibited plant growth, raising significant ecological concerns. Sustainable mitigation strategies, including bioremediation and advanced filtration systems, are proposed to reduce the environmental risks of AgNPs. This comprehensive analysis provides a framework for future ecological studies and regulatory measures, balancing the technological benefits of fungal-synthesized AgNPs with their environmental safety.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"101"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
3 BiotechPub Date : 2025-04-01Epub Date: 2025-03-03DOI: 10.1007/s13205-025-04240-6
Veintramuthu Sankar, Rajendran Selvakumar, R Narmadha, V G Jaishree
{"title":"Enhanced therapeutic approach for vaginal candidiasis: chitosan nanoparticulate thermoreversible in situ gels for sustained clotrimazole delivery.","authors":"Veintramuthu Sankar, Rajendran Selvakumar, R Narmadha, V G Jaishree","doi":"10.1007/s13205-025-04240-6","DOIUrl":"10.1007/s13205-025-04240-6","url":null,"abstract":"<p><p>This study aimed to develop and evaluate clotrimazole (CLZ)-loaded chitosan (CS) nanoparticles in a thermoreversible in situ gel for treating vaginal candidiasis (VC). Chitosan nanoparticles (CS-NPs) were prepared using ionotropic gelation with optimization through the design of experiments (DoE), considering factors such as chitosan pH, sodium tripolyphosphate (TPP) pH, the ratio of chitosan to TPP, and drug. Under optimal conditions (pH of CS, TPP, CS: TPP, and drug at 2, 2, 4:1, and 10 mg), nanoparticles exhibited desirable properties: particle size of 101.7 nm, polydispersity index (PDI) of 0.108, zeta potential of 35.4, and encapsulation efficiency of 98.36%. Thermoreversible in situ gels incorporating poloxamer (PXM) 407 and 188 were produced via the cold method and evaluated for mechanical and physicodynamic properties. It was found that nanoparticulate thermoreversible gel (NTG) prepared with 24% PXM 407, 4% PXM 188, 0.5% HPMC E-50, or 0.5% chitosan is suitable for vaginal administration, since it fulfills the in situ gel characteristics such as pH (4.7), gelation temperature and time (36 ℃ ± 0.2 and 4 ± 0.2 min), and viscosity (2690 cP (centipoise) at 25 ℃ and 15,600 cP at 37 ℃). In vitro release studies for the developed formulation showed 98% drug release over 72 h, with an extended residence time compared to the marketed formulation. In vitro antifungal and cytocompatibility studies revealed that the developed NTG was effective against VC and free from cytotoxicity.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"71"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876479/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}