巨噬细胞标记基因驱动的食管癌预后模型:整合多组学分析和治疗策略。

IF 2.9 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2025-10-01 Epub Date: 2025-09-04 DOI:10.1007/s13205-025-04452-w
Zehan Li, Huazhen Wu, Chuzhong Wei, Yunton Jia, Weiqi Zhao, Xiaoli Feng, Fanghui Bian, Pingmei Zhang, Qiyu Liao, Zhiyong Pan, Lingxin Zeng, Jiemin Liang, Yuxiao Tian, Xinyu Wang, Yuyi Liu, Xin Wang, Song Zhu, Ruiming Tang
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引用次数: 0

摘要

通过整合食管癌(ESCA)的单细胞和大量rna测序数据,我们开发并验证了7个巨噬细胞基因预后标记(FCN1, SCARB2, ATF5, PHLDA2, GLIPR1, CHORDC1和BCKDK)。这一特征有效地将患者分为高风险和低风险组,总生存率显著不同,实现了1年、2年和3年生存预测的曲线下面积(AUC)值大于0.7。高危状态与免疫抑制肿瘤微环境相关,其特征是B细胞和CD8 + T细胞的浸润较低,并与多种化疗药物(包括顺铂和5-氟尿嘧啶)的敏感性降低有关。相反,低风险状态与更大的免疫细胞浸润和更高的预测化学敏感性有关。在单细胞水平上,伪时间分析显示巨噬细胞成熟与风险评分降低显著相关,表明成熟的巨噬细胞可能有助于良好的预后。此外,细胞通讯分析发现,高风险巨噬细胞是通过信号通路(如SPP1和补体)促进致瘤微环境的主要驱动因素。总之,这7个基因标记是一个强大的预后生物标志物,为ESCA的个性化风险评估和治疗选择提供了新的策略。补充信息:在线版本包含补充资料,提供地址:10.1007/s13205-025-04452-w。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Macrophage marker gene-driven prognostic models for esophageal cancer: integrating multi-omics analysis and therapeutic strategies.

By integrating single-cell and bulk RNA-sequencing data for esophageal cancer (ESCA), we developed and validated a seven-macrophage-gene prognostic signature (FCN1, SCARB2, ATF5, PHLDA2, GLIPR1, CHORDC1, and BCKDK). This signature effectively stratified patients into high- and low-risk groups with significantly different overall survival, achieving area under the curve (AUC) values greater than 0.7 for 1-, 2-, and 3-year survival prediction. A high-risk status correlated with an immunosuppressive tumor microenvironment, characterized by lower infiltration of B cells and CD8 + T cells, and was associated with reduced sensitivity to multiple chemotherapeutic agents, including Cisplatin and 5-Fluorouracil. Conversely, a low-risk status was linked to greater immune cell infiltration and higher predicted chemosensitivity. At the single-cell level, pseudotime analysis revealed that macrophage maturation significantly correlated with a decreasing risk score, suggesting that mature macrophages may contribute to a favorable prognosis. Furthermore, cell communication analysis identified high-risk macrophages as dominant drivers of a pro-tumorigenic microenvironment via signaling pathways, such as SPP1 and complement. In conclusion, this seven-gene signature is a robust prognostic biomarker that offers a new strategy for personalized risk assessment and treatment selection in ESCA.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04452-w.

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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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