3 Biotech最新文献

{"title":"Exploring the protective role of metformin and dehydrozingerone in sodium fluoride-induced neurotoxicity: evidence from prenatal rat models.","authors":"Tejas Ahuja, Farmiza Begum, Gautam Kumar, Smita Shenoy, Nitesh Kumar, Rekha R Shenoy","doi":"10.1007/s13205-024-04175-4","DOIUrl":"10.1007/s13205-024-04175-4","url":null,"abstract":"<p><p>This study is aimed at evaluating the neurotoxic effects of chronic exposure of sodium fluoride (NaF) in developmental stages in rat using prenatal models. NaF (100 ppm, orally) dosing via drinking water was given to pregnant rats in disease group. In the treatment groups, Metformin & Dehydrozingerone (DHZ) (200 mg/kg) were administered orally along with NaF, and the dosing was continued throughout the gestation and lactation periods to the pups until the end of experiment. Behavioural studies like Novel Object Recognition Test (NORT), Open Field & Actophotometer test and biochemical estimations like Acetylcholinesterase (AchE), Glutathione (GSH), Malondialdehyde (MDA) were conducted on animals followed by histopathological image analysis. It was observed that NaF exposure significantly decreased learning, memory and locomotor ability (at p < 0.05, p ≤ 0.01) in rat pups and was also able to induce anxiety like behavior. Levels of AchE (p ≤ 0.001) and MDA (p ≤ 0.01, p ≤ 0.001) was found to be significantly elevated and GSH levels were significantly decreased (p ≤ 0.01, p ≤ 0.001) in hippocampus and frontal cortex in the disease group. Histopathological image analysis showed presence of degenerated neurons in hippocampus of disease group. From this study, it was observed that treatment with Metformin and DHZ, was able to significantly ameliorate the cognitive impairments, improve the condition of oxidative stress and decrease neuronal degeneration in NaF fed rat pups. These results established the protective role of Metformin and DHZ in NaF induced neurodevelopmental toxicity with particular emphasis on their antioxidant properties.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 2","pages":"36"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11711601/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microwave-synthesized NiZrO₃@GNP and NiZrO3@MWCNT nanocomposites: enhanced antimicrobial efficacy against biofilms and <i>Mycobacterium smegmatis</i>.","authors":"J John Benitto, J Judith Vijaya, Thenmozhli Geetha Saravanan, Radhakrishnan Manikkam, Bhavesh H Budhi","doi":"10.1007/s13205-024-04201-5","DOIUrl":"10.1007/s13205-024-04201-5","url":null,"abstract":"<p><p>The persistent challenge posed by antibiotic-resistant bacteria and tuberculosis necessitates innovative approaches to antimicrobial treatment. This study explores the synthesis and characterization of NiZrO₃ nanoparticles integrated with graphene nanoplatelets (GNP) and multi-walled carbon nanotubes (MWCNT), using a microwave-assisted green synthesis route, employing fenugreek (<i>Trigonella foenum-graecum</i>) seed extract as a gelling agent. The synthesised nanocomposites were systematically analyzed using XRD, FT-IR, Raman spectroscopy, HR-SEM and HR TEM analysis to assess structural, optical, and morphological properties. The antimicrobial and antibiofilm efficacy was evaluated against drug-resistant strains, including <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i>, by well diffusion method and crystal violet-Microtitre plate (CV-MtP) method. Notably, the NiZrO₃@MWCNT composite exhibited a maximum antibacterial inhibition zone of 13 mm and showed superior biofilm inhibition of 92.8% against K. pneumoniae at 500 ppm. In contrast, NiZrO₃@GNP demonstrated a biofilm inhibition of 97% at 500 ppm. Furthermore, the microplate Alamar Blue assay (MABA) was employed to determine the minimum inhibitory concentration (MIC) against <i>Mycobacterium smegmatis</i> (MTS) with NiZrO₃@MWCNT achieving 96% inhibition and at 500 ppm. These results confirm the enhanced antimicrobial efficacy of the carbon-integrated nanocomposites over pure NiZrO₃, which showed limited activity. This research underscores the promise of NiZrO₃-based nanocomposites as advanced antimicrobial agents, offering a novel strategy to combat the global health threat of antibiotic resistance.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04201-5.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 2","pages":"35"},"PeriodicalIF":2.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11707131/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of leads from bis-indole based triazine derivatives targeting human aldose reductase in diabetic type 2: in-silico approaches.","authors":"Miah Roney, Abdul Rashid Issahaku, Md Nazim Uddin, Anke Wilhelm, Mohd Fadhlizil Fasihi Mohd Aluwi","doi":"10.1007/s13205-024-04178-1","DOIUrl":"10.1007/s13205-024-04178-1","url":null,"abstract":"<p><p>Diabetes mellitus (DM) poses a major global healthcare challenge, highlighting the need for new treatments beyond current options. Currently available drugs have side effects including weight gain, nausea, vomiting, diarrhea, insulin resistance etc. Therefore, given the benefits of indole derivatives in diabetes and the lack of computational studies on bis-indole-based triazine derivatives with aldose reductase (AR), this study employs in-silico analysis to explore their potential as type-2 diabetes treatments. Based on the Differential Expression analysis, the human aldose reductase (HAR) encoding gene AKR1B1 showed overexpression in GSE30122 diabetes patients (Log2FC = 0.62, <i>P</i> < 0.01). Moreover, the compounds 2-((5,6-di(1H-indol-3-yl)-1,2,4-triazin-3-yl)thio)-1-(3-hydroxy-5-methylphenyl)ethan-1-one (4) and 2-((5,6-di(1H-indol-3-yl)-1,2,4-triazin-3-yl)thio)-1-(4-nitrophenyl)ethan-1-one (8) were identified as leading candidates, showing binding energies of - 62.12, - 81.73 kcal/mol and - 57.19, - 85.97 kcal/mol, respectively. Docking, MM/GBSA screening, molecular dynamics (MD) simulations, PCA, and post-MM/GBSA analysis confirmed their stability and favorable binding compared to the apo protein and control. Further in-vitro, in-vivo, and clinical studies are required to validate their therapeutic potential.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"5"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11635081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multilocus gene characterization of a '<i>Candidatus</i> Phytoplasma trifolii'-related strain (16SrVI-D) associated with witches' broom and stunting disease of <i>Gelbionis coronaria</i> (L.).","authors":"Priyam Panda, Shivani Gupta, Govind P Rao","doi":"10.1007/s13205-024-04198-x","DOIUrl":"10.1007/s13205-024-04198-x","url":null,"abstract":"<p><p>During November-December of 2019, severe witches' broom along with little leaf and stunting symptoms was observed in <i>Glebionis coronaria</i> at Indian Institute of Sugarcane Research, Lucknow, Uttar Pradesh with an average disease incidence of 20%. An amplicon of ~ 1.3 kb of 16S rRNA gene was amplified in symptomatic <i>C</i>. <i>coronarium</i> plants using universal phytoplasma-specific nested primer pairs (P1/P7 followed by 3Fwd/3Rev). Additionally, in another host, <i>Catharanthus roseus</i>, growing in near beds and the leafhopper species <i>Hishimonus phycitis</i>, amplification of similar amplified products were obtained using the similar nested PCR primer pairs. Further, phytoplasma presence was confirmed by amplifying non-ribosomal genes including <i>secA, rp</i> and <i>secY</i> in the symptomatic <i>G. coronaria, Catharanthus roseus</i> and <i>Hishimonus phycitis</i> using gene-specific primers of <i>secA</i>, <i>secY</i>, and <i>rp</i> genes. The comparison of 16S rRNA, <i>secA</i>, <i>rp</i>, and <i>secY</i> gene sequences through BLASTn, phylogeny and in silico RFLP analysis of <i>C. coronarium, C. roseus</i>, and the leafhopper species <i>Hishimonus phycitis</i> identified the presence of '<i>Candidatus Phytoplasma trifolii</i>'-related strain (16SrIV-D subgroup) in all the plants and leafhopper samples. These findings demonstrated the utility of multilocus genes for accurate identification of phytoplasma strains in the plant and insect samples. This is the first report of 16SrVI-D subgroup of phytoplasmas associated with witches' broom, little leaf and stunting disease in <i>Glebionis coronaria</i>.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"26"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682026/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enzymatic characterization of a thermostable 6-phosphogluconate dehydrogenase from <i>Hydrogenobacter thermophilus</i> and its application for NADH regeneration.","authors":"Xinming Feng, Xinyu Cui, Kun Wang, Juanjuan Liu, Dongdong Meng","doi":"10.1007/s13205-024-04165-6","DOIUrl":"10.1007/s13205-024-04165-6","url":null,"abstract":"<p><p>6-Phosphogluconate dehydrogenases (6PGDHs) are widely existing as reduced cofactor (NADH/NADPH) regeneration biocatalysts. Herein, a thermostable 6PGDH from <i>Hydrogenobacter thermophilus</i> (Ht6PGDH) was overexpressed in <i>Escherichia coli</i> and enzymologically characterized. Ht6PGDH exhibited exceptional stability and catalytic activity under high-temperature conditions, with an optimum temperature of 85 °C and the ability to maintain high activity for prolonged periods at 70 °C, which could be purified through a one-step heat treatment. Moreover, Ht6PGDH exhibited a preference for NAD⁺ with a <i>K</i> _m value of 0.4 mM and a <i>k</i> _cat value of 28.6 s⁻¹, demonstrating a significant preference over NADP⁺. These properties render Ht6PGDH a potentially valuable enzyme for high-temperature bioconversion and in vitro synthetic biosystems. Additional research showed that Ht6PGDH excelled in the regeneration of NADH, achieving efficient lactate production when integrated into an in vitro synthetic biosystem containing lactate dehydrogenase (LDH). Furthermore, the cascade reaction of Ht6PGDH with glucose-6-phosphate dehydrogenase (G6PDH) was explored for NADH regeneration using starch as the substrate, further validating its potential application in complex biosynthetic systems.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04165-6.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"3"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11621248/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Involvement of intestinal mucosal microbiota in adenine-induced liver function injury.","authors":"Leyao Fang, Junxi Shen, Yi Wu, Zhoujin Tan","doi":"10.1007/s13205-024-04180-7","DOIUrl":"10.1007/s13205-024-04180-7","url":null,"abstract":"<p><p>Adenine is frequently utilized as a model medication for chronic renal disease. Adenine can affect organs other than the kidneys, including the heart and the intestine. The liver is a vital organ involved in the in vivo metabolism of adenine. Adenine may negatively impact liver function. Research indicated that adenine caused dysbiosis of the gut microbiota in mice. Investigations into the gut-liver axis have demonstrated a substantial association between drug-induced hepatic dysfunction and gut microbiota. Consequently, we delivered distinct dosages of adenine via gavage to mice to examine the correlation between adenine-induced liver impairment and gut microbiota dysbiosis. Mice were treated with low-dose adenine suspension (NLA), medium-dose adenine suspension (NMA), high-dose adenine suspension (NHA), and sterile water (NC) as a control. The results indicated that mice in the NLA, NMA, and NHA groups had decreased body weight and a reduction in liver index. Subsequent to adenine administration, the concentrations of AST, ALT, and LDH increased, whereas SDH levels decreased. As doses increased, liver function impairment and hepatic energy metabolism abnormalities aggravated. Adenine also damaged the colonic architecture in mice. Moreover, adenine modified the makeup and structure of the gut mucosal microbiota, enhancing specific bacterial genera and influencing the microbiota's energy metabolism-related functions. The results of our research established a correlation among certain bacteria, liver function injury, and hepatic energy metabolism. The gut mucosal microbiota was involved in adenine-induced liver injury and hepatic energy metabolism. These results can offer novel insights into the role of gut microbiota in drug-induced liver injury and provide specific guidelines for the modeling and therapeutic application of adenine.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"6"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11638458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation, structure analysis and expression characterization of the Hexokinase gene family in <i>Sorghum bicolor</i>.","authors":"Sen Li, Yansheng Liu, Xin'er Qin, Xiaofei He, Shaopeng Han, Yang Lv, Zhuying Deng, Gongjian Zeng, Xinqiang Gao, Yongfeng Hu, Xiangling Shen","doi":"10.1007/s13205-024-04190-5","DOIUrl":"10.1007/s13205-024-04190-5","url":null,"abstract":"<p><p>Hexokinases (HXK) not only facilitate carbohydrate metabolism but also play important roles in sugar sensing in higher plants. <i>HXK</i> gene families have been extensively discussed in many plant species; however, comprehensive information regarding <i>HXKs</i> in sorghum remains unclear. To address this gap, we identified 7 putative sorghum <i>HXKs</i> (<i>SbHXK1</i> to <i>SbHXK7</i>), and the features of their conserved domains, gene structure, evolutionary tree, and cis-acting elements were systematically characterized to reveal the evolutionary conservation between different plant species. Based on expression profiling, we found that different expression patterns of <i>SbHXKs</i> were associated with different physiological processes, including abiotic stresses. Further qRT-PCR verification under salt and sucrose treatment confirmed that <i>SbHXK2</i>, <i>SbHXK4</i>, and <i>SbHXK5</i> may play very important roles under high osmotic pressure. Notably, SbHXKs are predominantly localized in the cytoplasm, in contrast to some rice and <i>Arabidopsis</i> HXKs, which are localized in chloroplasts or mitochondria, suggesting divergent roles for SbHXKs. In summary, our study provides a theoretical foundation for understanding the HXK gene family and offers fundamental insights of <i>SbHXKs</i> in sorghum.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04190-5.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"20"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11662118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical composition, in vitro cytotoxicity and in silico ADME/Tox analysis of the active compounds of <i>Oxalis latifolia</i> Kunth. extracts with promising anticancer potential.","authors":"Arumugam Vignesh, Karuppasamy Dharani, Subramaniam Selvakumar, Krishnan Vasanth","doi":"10.1007/s13205-024-04167-4","DOIUrl":"10.1007/s13205-024-04167-4","url":null,"abstract":"<p><p>This study investigated the anticancer phytocompounds in leaf extracts of <i>Oxalis latifolia</i> Kunth. Quantitative analysis of the phytochemical composition showed high levels of primary metabolites: carbohydrates (45.11 ± 2.15 GLE mg/100 mg), proteins (28.13 ± 0.94 BSA mg/100 mg), and amino acids (13.25 ± 1.16 LE mg/100 mg). Ethyl acetate extracts had the highest concentrations of secondary metabolites, including phenolic content (122.52 ± 4.27 GAE mg/100 mg), total flavonoids (91.86 ± 2.65 QE mg/100 mg), and alkaloids (82.18 ± 0.72 COLE mg/100 mg). In addition, strong antioxidant activities were observed in the DPPH^• scavenging assay (IC₅₀ 11.51 ± 2.28 µg/mL), ABTS^·+ radical cation scavenging activity (97.42 ± 7.19 µM TE/g), and FRAP assay (14.34 ± 1.24 mM Fe(II)/mg). Based on preliminary analysis, the ethyl acetate extract was fractionated using thin-layer chromatography (TLC), yielding two distinct fractions with Rf values of 0.31 and 0.76. GC-MS analysis of these fractions identified 33 bioactive compounds. These fractions exhibited anticancer activity against the A549 lung cancer cell line, with IC₅₀ values of 47.25 µg/mL and 48.31 µg/mL, as determined by the MTT assay. Furthermore, absorption, distribution, metabolism, excretion, and toxicity (ADMET) studies on 25 selected compounds indicated favorable pharmacokinetic properties and drug-likeness. In silico molecular docking showed strong binding affinities of these bioactive compounds to the p21 protein, comparable to the synthetic drug Cisplatin-quercetin. The results highlight the potential of <i>O. latifolia</i> in anticancer therapy, particularly through modulation of the p21 pathway, supported by in vitro cytotoxicity assessments, molecular docking, and ADMET analysis.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04167-4.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"19"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11662119/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome sequencing of <i>Escherichia coli</i> phage <i>UFJF_EcSW4</i> reveals a novel lytic <i>Kayfunavirus</i> species.","authors":"Pedro Marcus Pereira Vidigal, Humberto Moreira Hungaro","doi":"10.1007/s13205-024-04172-7","DOIUrl":"10.1007/s13205-024-04172-7","url":null,"abstract":"<p><p>The <i>Escherichia coli</i> phage <i>UFJF_EcSW4</i> was isolated from polluted stream water and showed clear lysis plaques on the host, measuring 0.67 ± 0.43 mm, with a titer of 9.57 ± 0.23 log PFU/ml. It demonstrated a very narrow host range, infecting only its host. Additionally, it has a short latent period of 9 min, a burst size of 49 PFU/infected cell, and stability over a wide range of pH, temperature, and free residual chlorine. The phage has a double-stranded DNA genome spanning 40,299 bp, with a GC content of 49.87% and short-direct terminal repeats (DTR) sequences of 286 bp. The <i>UFJF_EcSW4</i> genome contains 55 genes, organized into functional modules with a unidirectional arrangement, regulated by 22 promoters (three from the phage and 19 from the host) and three Rho-independent terminators. Comparative analysis revealed that the <i>UFJF_EcSW4</i> genome shares an average genomic similarity of 77.82% with the genome sequences of phages from the <i>Kayfunavirus</i> genus but does not surpass the 95% threshold necessary for species classification. Therefore, the <i>UFJF_EcSW4</i> is a novel <i>Kayfunavirus UFJF_EcSW4</i> species belonging to the <i>Studiervirinae</i> subfamily.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04172-7.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"10"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11646959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142845503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the neuroprotective properties of <i>Sargassum wightii</i> extract against MPP⁺-induced apoptosis in SH-SY5Y human neuroblastoma cells.","authors":"Rajeswari Ramasamy, Muthukumaran Azhaguchamy, Johnson Retnaraj Samuel Selvan Christyraj, Lalithalakshmi Kanagaraj","doi":"10.1007/s13205-024-04185-2","DOIUrl":"10.1007/s13205-024-04185-2","url":null,"abstract":"<p><p>This study aims to assess the neuroprotective effects of the methanolic extract of <i>Sargassum wightii</i> against oxidative stress and cell death induced by neurotoxins MPP ⁺ in SH-SY5Y cells. Briefly, the methanolic extract of <i>S.wightii</i> decreased the cytotoxicity of MPP ⁺ in SH-SY5Y cells. Treatment with <i>S.wightii</i> extract at a concentration of 400 µg/ml resulted in a notable decrease in cell death, particularly in MPP ⁺ -induced cells. Flow cytometry analysis with annexin V/PI staining reveals apoptosis and necrosis in SH-SY5Y cell lines upon exposure to 1 mM of MPP ⁺ . However, 100-400 µg/ml concentrations of <i>S.wightii</i> extract effectively decrease apoptosis in SH-SY5Y cells. Furthermore, <i>S.wightii</i> inhibits caspase-3 activity, effectively shielding neuronal cells against MPP ⁺ -induced cell death. Mitochondrial membrane potential (MMP) assay using a JC-1 fluorescent probe indicates that the methanolic extract of <i>S.wightii</i> exhibits protective effects against MPP ⁺ -induced cell death and maintains mitochondrial membrane potential. Our results conclude that exposing SH-SY5Y cells to a methanolic extract of <i>S.wightii</i> could potentially increase the likelihood of inhibiting the cascade mechanism, stopping MPP⁺-induced apoptosis, and preventing the rupture of the mitochondrial membrane. However, the lack of low solubility and poor bioavailability reduce the therapeutic efficacy of <i>S.wightii</i>. Liposome-based drug delivery systems can improve the bioavailability and stability of bioactive compounds, enhancing their therapeutic potential. Hence, <i>S.wightii</i> may hold promise as an innovative treatment for neurological ailments.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"22"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11668720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142891269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}