IF 2.6 4区生物学

3 Biotech Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI: 10.1007/s13205-024-04125-0

Achasih Q Nkemzi, Kunle Okaiyeto, Omolola Oyenihi, Chinyerum S Opuwari, Okobi E Ekpo, Oluwafemi O Oguntibeju

{"title":"Antidiabetic, anti-inflammatory, antioxidant, and cytotoxicity potentials of green-synthesized zinc oxide nanoparticles using the aqueous extract of Helichrysum cymosum.","authors":"Achasih Q Nkemzi, Kunle Okaiyeto, Omolola Oyenihi, Chinyerum S Opuwari, Okobi E Ekpo, Oluwafemi O Oguntibeju","doi":"10.1007/s13205-024-04125-0","DOIUrl":"10.1007/s13205-024-04125-0","url":null,"abstract":"The current research involved the synthesis of zinc oxide nanoparticles (ZnO-NPs) using an aqueous extract of Helichrysum cymosum shoots, and subsequent characterization via different analytical methods, such as UV-Vis spectroscopy, Scanning electron microscope (SEM), Energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Transmission electron microscope (TEM), and zeta potential. The biological effects of the ZnO-NPs were then tested against C3A hepatocyte cells and L6 myocyte cell lines via series of analysis, including cytotoxicity, antioxidant, anti-inflammatory, and antidiabetic effect via enzymatic inhibition. The UV-Vis analysis showed a maximum absorption spectrum at 360, and the TEM analysis reveals a spherical and hexagonal structures, with an average dimension of 28.05-58.3 nm, and the XRD reveals a crystalline hexagonal structure. The zeta potential evaluation indicated that the ZnO-NPs are relatively stable at - 20 mV, and the FTIR analysis identified some important functional group associated with phenolics, carboxylic acid, and amides that are responsible for reducing and stabilizing the ZnO-NPs. The synthesized ZnO-NPs demonstrated cytotoxic effects on the cell lines at higher concentrations (125 µg/mL and 250 µg/mL), complicating the interpretation of the results of the inflammatory and antioxidant assays. However, there was a significant (p < 0.05) increase in the inhibitions of pancreatic lipase, alpha-glucosidase, and alpha-amylase, indicating beneficial antidiabetic effects.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11535088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanomaterial-enabled drug transport systems: a comprehensive exploration of current developments and future avenues in therapeutic delivery. 纳米材料药物传输系统：全面探索治疗传输的当前发展和未来途径。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI: 10.1007/s13205-024-04135-y

Shatabdi Basu, Pragnya Biswas, Mariya Anto, Nandini Singh, Koel Mukherjee

{"title":"Nanomaterial-enabled drug transport systems: a comprehensive exploration of current developments and future avenues in therapeutic delivery.","authors":"Shatabdi Basu, Pragnya Biswas, Mariya Anto, Nandini Singh, Koel Mukherjee","doi":"10.1007/s13205-024-04135-y","DOIUrl":"10.1007/s13205-024-04135-y","url":null,"abstract":"Over the years, nanotechnology has gained popularity as a viable solution to address gene and drug delivery challenges over conventional methods. Extensive research has been conducted on nanosystems that consist of organic/inorganic materials, drugs, and its biocompatibility become the primary goal of improving drug delivery. Various surface modification methods help focus targeted and controlled drug release, further enabling multidrug delivery also. This newer technology ensures the stability of drugs that can unravel the mechanisms involved in cellular processes of disease development and its management. Tailored medication delivery provides benefits such as therapy, controlled release, and reduced adverse effects, which are especially important for controlling illnesses like cancer. However, multifunctional nanocarriers that possess high viscoelasticity, extended circulation half-life, biocompatibility, and biodegradability face some challenges and limitations too in human bodies. To produce a consistent therapeutic platform based on complex three-dimensional nanoparticles, careful design and engineering, thorough orthogonal analysis methods, and reproducible scale-up and manufacturing processes will be required in the future. Safety and effectiveness of nano-based drug delivery should be thoroughly investigated in preclinical and clinical trials, especially when considering biodistribution, targeting specific areas, and potential immunological toxicities. Overall, the current review article explores the advancements in nanotechnology, specific to nanomaterial-enabled drug delivery systems, carrier fabrication techniques and modifications, disease management, clinical research, applications, limitations, and future challenges. The work portrays how nanomedicine distribution affects healthcare with an emphasis on the developments in drug delivery techniques.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinformatics analysis and experimental validation of the oncogenic role of COL11A1 in pan-cancer. COL11A1在泛癌症中致癌作用的生物信息学分析和实验验证。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI: 10.1007/s13205-024-04133-0

Xiaofeng Wan, Qingmei Deng, Anling Chen, Xinhui Zhang, Wulin Yang

{"title":"Bioinformatics analysis and experimental validation of the oncogenic role of COL11A1 in pan-cancer.","authors":"Xiaofeng Wan, Qingmei Deng, Anling Chen, Xinhui Zhang, Wulin Yang","doi":"10.1007/s13205-024-04133-0","DOIUrl":"10.1007/s13205-024-04133-0","url":null,"abstract":"The intricate expression patterns and oncogenic attributes of COL11A1 across different cancer types remain largely elusive. This study used several public databases (TCGA, GTEx, and CCLE) to investigate the pan-cancer landscape of COL11A1 expression, its prognostic implications, interplay with the immune microenvironment, and enriched signaling cascades. Concurrently, western blot analyses were performed to verify COL11A1 expression in lung adenocarcinoma (LUAD) cell lines and clinical samples. In addition, COL11A1 knockout cell lines were generated to scrutinize the functional consequences of COL11AI expression on cancer cell behavior by use MTT, colony formation, and scratch wound healing assays. A comprehensive database investigation revealed that COL11A1 was upregulated in a majority of tumor tissues and its expression was highly correlated with a patient's prognosis. Notably, genetic alterations in COL11A1 predominantly occurred as mutations, while its DNA methylation status inversely mirrored gene expression levels across multiple promoter regions. Our findings suggest that COL11A1 helps to modulate the tumor immune landscape and potentially acts through the epithelial-mesenchymal transition (EMT) pathway to exert its oncogenic function. Western blot analyses further substantiated the specific upregulation of COL11A1 in LUAD cell lines and tissues, suggesting a close association with the EMT process. Ablation of COL11A1 in cancer cells significantly reduced their proliferative, clonogenic, and migratory abilities, underscoring the functional significance of COL11A1 in tumor cell behavior. Collectively, this research revealed the prevalent overexpression of COL11A1 in pan-cancer tissues, its profound prognostic and microenvironmental correlations, and the mechanistic underpinnings of its tumor-promoting effects as mediated via EMT signaling. Our findings suggest that COL11A1 could serve as a prognostic and diagnostic biomarker and therapeutic target for cancer.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in the synthesis of antidepressant derivatives: pharmacologic insights for mood disorders. 抗抑郁衍生物合成的最新进展：情绪障碍的药理学启示。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-11-01 Epub Date: 2024-10-05 DOI: 10.1007/s13205-024-04104-5

Jeetendra Kumar Gupta, Kuldeep Singh, Alok Bhatt, Prateek Porwal, Rekha Rani, Anubhav Dubey, Divya Jain, Sachchida Nand Rai

{"title":"Recent advances in the synthesis of antidepressant derivatives: pharmacologic insights for mood disorders.","authors":"Jeetendra Kumar Gupta, Kuldeep Singh, Alok Bhatt, Prateek Porwal, Rekha Rani, Anubhav Dubey, Divya Jain, Sachchida Nand Rai","doi":"10.1007/s13205-024-04104-5","DOIUrl":"10.1007/s13205-024-04104-5","url":null,"abstract":"Mood disorders, including depression, remain a significant global health concern, necessitating continuous efforts to develop novel and more effective antidepressant therapies. Although there have been significant advancements in comprehending the biology of Major Depressive Disorder (MDD), a considerable number of people suffering from depression do not exhibit positive responses to the pharmacologic treatments now available. This study specifically examines emerging targets and potential future approaches for pharmaceutical interventions in the treatment of MDD. The discussion revolves around novel therapeutic agents and their effectiveness in treating depression. The focus is on the specific pathophysiological pathways targeted by these agents and the amount of evidence supporting their use. While conventional antidepressants are anticipated to continue being the primary treatment for MDD in the foreseeable future, there is currently extensive research being conducted on numerous new compounds to determine their effectiveness in treating MDD. Many of these compounds have shown encouraging results. This review highlighted the recent advances in the synthesis of antidepressant derivatives and explores their pharmacologic insights for the treatment of mood disorders.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11456089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142396915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing epigenetic profiling in cervical cancer: machine learning techniques for classifying DNA methylation patterns. 推进宫颈癌的表观遗传学分析：对 DNA 甲基化模式进行分类的机器学习技术。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-11-01 Epub Date: 2024-10-09 DOI: 10.1007/s13205-024-04107-2

Apoorva, Vikas Handa, Shalini Batra, Vinay Arora

{"title":"Advancing epigenetic profiling in cervical cancer: machine learning techniques for classifying DNA methylation patterns.","authors":"Apoorva, Vikas Handa, Shalini Batra, Vinay Arora","doi":"10.1007/s13205-024-04107-2","DOIUrl":"10.1007/s13205-024-04107-2","url":null,"abstract":"This study investigates the ability to predict DNA methylation patterns in cervical cancer cells using decision-tree-based ensemble approaches and neural network-based models. The research findings suggest that a model based on random forest achieves a significant prediction accuracy of 91.35%. This projection was derived from comprehensive experimentation and a meticulous performance evaluation of the random forest model, employing a range of measures including Accuracy, Sensitivity, Specificity, Matthews Correlation Coefficient, F1-score, Recall, and Precision. The results indicate that the random forest model exhibits superior performance compared to other tree-based models such as the Simple Decision Tree and XGBoost, as well as neural network-based models including Convolutional Neural Networks, Feed Forward Networks, and Wavelet Neural Networks. The findings indicate that using random forest-based techniques has great potential for future study and might be highly valuable in clinical applications, especially in improving diagnostic and treatment strategies based on epigenetic profiles.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11461404/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biohydrogen production from co-substrates through dark fermentation by bacterial consortium. 细菌联合体通过暗发酵从共底物中生产生物氢。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-11-01 Epub Date: 2024-10-25 DOI: 10.1007/s13205-024-04106-3

Chelladurai Mumtha, Pambayan Ulagan Mahalingam

{"title":"Biohydrogen production from co-substrates through dark fermentation by bacterial consortium.","authors":"Chelladurai Mumtha, Pambayan Ulagan Mahalingam","doi":"10.1007/s13205-024-04106-3","DOIUrl":"10.1007/s13205-024-04106-3","url":null,"abstract":"Hydrogen is a clean energy carrier that can be used as fuel for fuel cells. Dark fermentative biohydrogen production with other waste biomass needs to be explored as an alternative for sustainable biohydrogen production in future. In this study, lab-scale bioreactor were carried out to produce biohydrogen from co-substrates using bacterial consortium at 37 ℃. For the experimental setup, a 1-L-working-volume reactor was used for biohydrogen production by bacterial monocultures and consortium on co-substrates. A batch experiment was performed at 37 °C with an initial pH of 7.0 and a mixing ratio of 600:300 between dairy whey and sugarcane bagasse. Total solids (TS), volatile solids (VS), total chemical oxygen demand (TCOD), soluble chemical oxygen demand (SCOD), and hydrogen production rate (HPR) were determined from co-substrates during the dark fermentation process. Morphologic changes of biohydrogen producing bacteria binds on co-substrates after the fermentation process were determined using SEM imaging. The bacteria can degrade the substrate when they attach to it causing hole formation and cracked the surface area. The level of biohydrogen production by bacterial consortium was observed and the results revealed a hydrogen production rate of 35.9 mL H2/L/h. In fermentative H2 production, it is quite similar to that of most H2-producing bacteria previously studied, especially that of the bacterial consortium, and this indicates that the attempt to find an outstanding bacterial strain for fermentative H2 production might be very difficult if not impossible.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial Expression of Concern: Two species of Ulva inhibits the progression of cervical cancer cells SiHa by means of autophagic cell death induction. 社论表达关注：两种莼菜通过诱导自噬细胞死亡抑制宫颈癌细胞SiHa的进展。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-11-01 Epub Date: 2024-10-24 DOI: 10.1007/s13205-024-04115-2

Asmita Pal, Preeti Verma, Subhabrata Paul, Indira Majumder, Rita Kundu

引用次数: 0

Correction: Genome-wide identification and characterization of glutathione S-transferase gene family in quinoa (Chenopodium quinoa Willd.). 更正：藜麦（Chenopodium quinoa Willd.）

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-11-01 Epub Date: 2024-10-12 DOI: 10.1007/s13205-024-04076-6

Shivani Tiwari, Swati Vaish, Nootan Singh, Mahesh Basantani, Atul Bhargava

引用次数: 0

Pharmacokinetic predictions of ROS-mediated targets and genotoxin combinations via multiple ligand simultaneous docking and ROS evaluation in vitro using HepG2 cell lines. 利用 HepG2 细胞系，通过多配体同时对接和 ROS 体外评估，对 ROS 介导的靶标和基因毒素组合进行药代动力学预测。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-11-01 Epub Date: 2024-10-14 DOI: 10.1007/s13205-024-04109-0

C P Sri Snehaa, Praveen Kumar Issac, Palanisamy Rajaguru, Velan Pugalenthi

{"title":"Pharmacokinetic predictions of ROS-mediated targets and genotoxin combinations via multiple ligand simultaneous docking and ROS evaluation in vitro using HepG2 cell lines.","authors":"C P Sri Snehaa, Praveen Kumar Issac, Palanisamy Rajaguru, Velan Pugalenthi","doi":"10.1007/s13205-024-04109-0","DOIUrl":"10.1007/s13205-024-04109-0","url":null,"abstract":"Although combination therapy is known for its high efficacy, reduced side effects and drug resistance, toxicity remains a major drawback. Some of the genes are likely to induce hepatotoxicity through ROS-mediated mechanisms when a drug is metabolized alone or in combination in the liver. To address this, we have developed a scientific approach to predict the toxicity of different genotoxin combinations and validate their interactions with various targets. The current study is an extensive study of our previous set of in vivo rat liver microarray data processed using R studio for their functional analysis. About five combinations of genotoxins such as CPT/ETP, CPT/CPL, ETP/CPL, CP/CPT and EES/CP along with their differential gene expression targeting Chemical carcinogenesis-ROS are chosen for this study. We aim to examine the binding affinity of different genotoxin combinations using in silico multiple ligand simultaneous docking (MLSD) and are then bio-evaluated for cytotoxicity in vitro using human hepatocellular carcinoma cell lines (HepG2) with the MTT assay. As a result, dose-response cytotoxicity with its strength of interactions and a significant variance in ROS levels in the treated cells is observed compared to their IC50 values. Out of 5 combinations such as CPT/CPL, ETP/CPL and EES/CP are found not only to be significantly cytotoxic but also induce oxidative stress specifically above their IC50 values with good and moderate binding interactions ensuring their toxicity. On the contrary, the safe combinations are found to be CTP/ETP and CP/CPT possibly with no and tolerable adverse effects standing as preliminary information for researchers in drug design and development.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11471743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142455458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing glioblastoma cytotoxicity through encapsulating O6-benzylguanine and temozolomide in PEGylated liposomal nanocarrier: an in vitro study. 通过在 PEG 化脂质体纳米载体中封装 O6-苄基鸟嘌呤和替莫唑胺来增强胶质母细胞瘤的细胞毒性：一项体外研究。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-11-01 Epub Date: 2024-10-23 DOI: 10.1007/s13205-024-04123-2

Manasa Manjunath Hegde, Pranoti Palkar, Sadhana P Mutalik, Srinivas Mutalik, Jayant Sastri Goda, B S Satish Rao

{"title":"Enhancing glioblastoma cytotoxicity through encapsulating O6-benzylguanine and temozolomide in PEGylated liposomal nanocarrier: an in vitro study.","authors":"Manasa Manjunath Hegde, Pranoti Palkar, Sadhana P Mutalik, Srinivas Mutalik, Jayant Sastri Goda, B S Satish Rao","doi":"10.1007/s13205-024-04123-2","DOIUrl":"10.1007/s13205-024-04123-2","url":null,"abstract":"Glioblastoma (GBM) (grade IV glioma) is the most fatal brain tumor, with a median survival of just 14 months despite current treatments. Temozolomide (TMZ), an alkylating agent used with radiation, faces challenges such as systemic toxicity, poor absorption, and drug resistance. To enhance TMZ effectiveness, we developed poly(ethylene glycol) (PEG) liposomes co-loaded with TMZ and O6-benzylguanine (O6-BG) for targeted glioma therapy. These liposomes, prepared using the thin-layer hydration method, had an average size of 146.33 ± 6.75 nm and a negative zeta potential (-49.6 ± 3.1 mV). Drug release was slower at physiological pH, with 66.84 ± 4.62% of TMZ and 69.70 ± 2.88% of O6-BG released, indicating stability at physiological conditions. The liposomes showed significantly higher cellular uptake (p < 0.05) than the free dye. The dual drug-loaded liposomes exhibited superior cytotoxicity against U87 glioma cells, with a lower IC50 value (3.99µg/mL) than the free drug combination, demonstrating enhanced anticancer efficacy. The liposome formulation induced higher apoptosis (19.42 ± 3.5%) by causing sub-G0/G1 cell cycle arrest. The novelty of our study lies in co-encapsulating TMZ and O6-BG within PEGylated liposomes, effectively overcoming drug resistance and improving targeted delivery for glioma treatment.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04123-2.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11499494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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