3 Biotech最新文献

{"title":"Biotransformation of baicalin and glycyrrhizic acid using immobilized Fe₃O₄@Chitosan@β-glucuronidase.","authors":"Yue Zhao, Weiqiang Zhou, Ping Wang, Yumei Li, Pengfei Gu, Juan Gao","doi":"10.1007/s13205-025-04220-w","DOIUrl":"https://doi.org/10.1007/s13205-025-04220-w","url":null,"abstract":"<p><p>β-Glucuronidase can hydrolyze β-glucuronic acid-containing glycosides, such as baicalin and glycyrrhizic acid. In this study, the β-glucuronidase gene from <i>Lactobacillus rhamnosus</i> was cloned and expressed in <i>Escherichia coli</i>. The resulting recombinant protein, designated LrhGUS, exhibited a molecular weight of approximately 72 kDa. The hydrolysis pathway of glycyrrhizic acid by recombinant LrhGUS proceeded as follows: glycyrrhizic acid → glycyrrhetinic acid monoglucuronide (GMAG) → glycyrrhetinic acid (GA), achieving a conversion rate of 90.38% with 2 mg/ml glycyrrhizic acid. Additionally, LrhGUS hydrolyzed baicalin into baicalein with a conversion rate of 94.64% using 20 mg/ml baicalin. Magnetic chitosan microspheres were utilized as carriers for immobilizing recombinant LrhGUS. Response surface methodology was employed to optimize immobilization conditions, which were determined to be a glutaraldehyde concentration of 1%, an enzyme loading of 0.8 mg/g bead, and a crosslinking temperature of 25 °C. The optimal temperature and pH for the immobilized enzyme were identical to those of the free enzyme; however, the immobilized enzyme demonstrated superior stability compared to the free enzyme. Notably, under acidic conditions, the pH stability of immobilized LrhGUS was significantly higher than that of the free enzyme. After incubation at 80 °C for 12 h, the thermal stability of the immobilized enzyme improved by approximately 50% relative to the free enzyme. Moreover, the immobilized LrhGUS exhibited excellent reusability, maintaining approximately 30% enzyme activity after seven cycles. Using the immobilized enzyme, baicalein was successfully prepared on a 1 g scale, while GAMG and GA were prepared on a 100 mg scale. These findings provide a robust foundation for the potential industrial application of β-glucuronidase.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04220-w.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"63"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11829881/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143439367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the therapeutic potential of oleanolic acid and its derivatives in cancer treatment: a comprehensive review.","authors":"Rachel Savio D'Mello, Vividh Mendon, Padmini Pai, Ipshita Das, Babitha Kampa Sundara","doi":"10.1007/s13205-025-04209-5","DOIUrl":"10.1007/s13205-025-04209-5","url":null,"abstract":"<p><p>Oleanolic acid (OA) is a triterpenoid that occurs naturally and may be isolated from various plants. Analogs of oleanolic acid can be produced artificially or naturally. The current treatments have limited selectivity and may also impact normal cells. OA and its derivatives provide a promising cancer treatment platform with greater selectivity and less toxic effects. As a result of their enhanced sensitivity, selectivity, and low toxicity, they are great options for focusing on particular biological pathways and reducing the growth of tumor cells. The effects of OA and derivatives of OA on various cancer types have been investigated. However, breast and hepatocellular malignancies are the most studied cancers. In breast cancer, derivatives such as saikosaponin A (SSa), saikosaponin B (SSb), and SZC014 influence key pathways such as the Janus kinase/signal transducer and activator of transcription (JAK/STAT), protein kinase-B (Akt), and nuclear factor-kappa B (NF-κB) pathways, inhibiting metastasis, angiogenesis, and cell migration, respectively. When a para-aminobenzoic acid (PABA)/nitric oxide (NO) derivative of OA is administered to HepG2 cells, the reactive oxygen species (ROS)/mitogen-activated protein kinase (MAPK)-mediated mitochondrial pathway causes apoptosis. Nanoformulations incorporating OA, such as OA-paclitaxel (PTX), show potential for suppressing tumor progression by inhibiting drug efflux mechanisms. Thus, exploring the interactions of OA and a few of its derivatives with various cellular pathways offers a promising approach to combating different types of cancer. This review delves into the potential of oleanolic acid and its derivatives in retarding cancer progression through their interactions with diverse cellular pathways.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"56"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11803024/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Streptococcus mutans</i> biofilms in the establishment of dental caries: a review.","authors":"Thangavelu Thayumanavan, B S Harish, Rathinasamy Subashkumar, Karuppusamy Shanmugapriya, Velusamy Karthik","doi":"10.1007/s13205-025-04227-3","DOIUrl":"10.1007/s13205-025-04227-3","url":null,"abstract":"<p><p>Dental caries is considered as the most common and multifactorial disease worldwide, caused by a variety of oral microorganisms like <i>Streptococcus</i> spp., <i>Veillonella</i> spp., <i>Actinomyces</i> spp., <i>Bifidobacterium</i> spp., and <i>Lactobacillus fermentum</i>, which colonize food debris in oral cavities. Of them, <i>Streptococcus mutans</i> is the predominant bacterium and can induce progressive tooth destruction, especially during dentition. The superior characteristics of <i>S. mutans</i>, such as the presence of the cell surface protein P1 and exopolysaccharide-synthesizing enzymes, acid tolerance, biofilm-forming ability mediated by <i>brp</i>A gene, and multidrug resistance, render it a highly virulent pathogen in the etiology of dental caries. Given its significant role in dental caries, extensive research has been conducted over the past few decades, focusing on the development of specific antimicrobial treatments, and other innovative therapeutic approaches. To gain deeper insights into the genetic diversity and epidemiological patterns of <i>S. mutans</i>, various genotypic methods have been developed and successfully employed. By combining the insights gained from genetic studies of <i>S. mutans</i> with the suitable control measures against the biofilm, we can develop innovative and effective strategies for preventing and treating dental caries.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"62"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11828782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a chloroquine hydrogel for topical treatment of leishmaniasis in BALB/c mice infected with <i>Leishmania</i> (<i>L.</i>) <i>amazonensis</i>.","authors":"Arlindo Gonzaga Branco Junior, Saara Neri Fialho, Aurileya de Jesus Gouveia, Amália Dos Santos Ferreira, Leandro Do Nascimento Martinez, Welington da Silva Paula do Nascimento, Norton Rubens Diunior Lucas Pejara Rossi, Minelly Azevedo da Silva, Mariana Francy Malaquias Lemke, Karine Modolon Zepon, Luiz Alberto Kanis, Laerth Dos Santos Ribeiro, Carolina Bioni Garcia Teles","doi":"10.1007/s13205-025-04216-6","DOIUrl":"10.1007/s13205-025-04216-6","url":null,"abstract":"<p><p>Leishmaniasis, a challenging disease to treat due to the intracellular location of the parasite and host immune modulation, requires innovative treatment solutions. This study evaluates a chloroquine-based hydrogel for topical use, targeting a localized and less invasive approach to leishmaniasis treatment. Thus, this study aimed at evaluating the skin permeation potential of the antimalarial chloroquine and also to evaluate the less invasive, topical treatment in murine models infected by <i>Leishmania (Leishmania) amazonensis</i>. For this end, a formulation was created, containing chloroquine (CQ), which had its release profile assessed through the ex vivo skin permeation assay using <i>Sus scrofa domesticus</i> skin. Afterward, the susceptibility profile of <i>L.</i> (<i>L.</i>) <i>amazonensis</i> in vivo in BALB/c mice was assessed over a 15-day treatment period. This study highlights the potential of chloroquine hydrogel as a topical treatment for tegumentary leishmaniasis, with significant reductions in parasite load and minimal side effects observed. The formulation demonstrated a 15% permeation rate during 12 h, with 20.26% on the 42nd day. A significant reduction in parasite load was observed in the treatment group. Thus, the results presented here point to the need for further pre-clinical trials with a higher concentration of the drug, other formulations and against other Brazilian species of Leishmania. Future studies should explore higher drug concentrations, alternative formulations, and other Leishmania species to expand the therapeutic potential of this approach.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"54"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11799487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relevance of proteomics and metabolomics approaches to overview the tumorigenesis and better management of cancer.","authors":"Pooja Singh, Yashika W Dhir, Shagun Gupta, Ankur Kaushal, Deepak Kala, Rupak Nagraiik, Naveen K Kaushik, Md Salik Noorani, Abdul R Asif, Bharat Singh, Shahbaz Aman, Sunny Dhir","doi":"10.1007/s13205-025-04222-8","DOIUrl":"10.1007/s13205-025-04222-8","url":null,"abstract":"<p><p>Proteomics and metabolomics, integral combination of OMICs platform are gaining prominence in cancer research to enhance scientific knowledge of bio-molecular interactions occurs in the cellular processes during cancer progression. This approach designed to identify potential tools for addressing the complexities of this multifaceted disease. This analysis focussed on the intricate interplay between proteins and metabolites within cancer cells and their surrounding microenvironment. By reviewing current proteomics and metabolomics studies, we aim to gain invaluable insights into tumour biology, progression, and its implication in therapeutic responses. This study highlights the importance of proteomics and metabolomics in discovering therapeutic targets and diagnostic biomarkers for targeted cancer treatment. Proteomics facilitates the analysis of protein expression, modifications and interactions, exemplified by the identification of HER2 mutations leads to development of breast cancer hence targeted therapies like trastuzumab could be initiated. Metabolomics reveals metabolic alternations such as elevated 2-hydroxyglutarate levels in gliomas linked to cancer progression and treatment resistance. The integration of these approaches clarifies complex signalling network driving oncogenesis and paves the way for innovative cancer therapies, including immune cheque point inhibitors. Proteomics and metabolomics have revolutionised cancer biology by revealing intricate signalling networks, metabolic dysregulations, and unique molecular alterations. This information is crucial for early cancer identification and prognosis, and for designing personalized therapeutic strategies. Innovative technologies like artificial intelligence and high-throughput mass spectrometry further enhance the potential of these studies. Fostering multidisciplinary collaboration and data-sharing is essential for maximising the impact of these approaches to cure as well as better management of the cancer.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"58"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors associated with tooth loss in patients with stages 3 and 4 periodontitis: a short-term retrospective evaluation of patients.","authors":"See Hyung Lee, Angela Chan, Tia Griffith, Lavanya A Sharma, Seyed Ebrahim Alavi, Nigel Robb, Ajay Sharma","doi":"10.1007/s13205-025-04217-5","DOIUrl":"10.1007/s13205-025-04217-5","url":null,"abstract":"<p><p>This retrospective study analyzed factors associated with tooth loss in patients with stages 3 and 4 periodontitis undergoing non-surgical periodontal therapy. Data from 84 patients treated at Griffith University Periodontal Clinic (2019-2022) were examined, focusing on patient factors such as smoking status, osteoporosis, and age, alongside tooth loss variables. The logistic regression analysis revealed that patients with stage 4 periodontitis had a significantly higher likelihood of tooth loss compared to those with stage 3 (odds ratio [OR] 2.12; 95% confidence interval [CI] 1.13-4.84). Smoking was also identified as a significant risk factor, with smokers showing an OR of 1.69 (95% CI 1.4-3.9) for tooth loss. While no statistically significant relationships were observed for other variables (<i>p</i> > 0.05), patients under 40 years and over 71 years with stage 4 periodontitis exhibited notable tooth loss outcomes (<i>p</i> = 0.003 and <i>p</i> = 0.034, respectively). These findings emphasize the importance of integrating smoking cessation programs into periodontal care and tailoring treatment strategies for high-risk groups. Further longitudinal studies with comprehensive data collection are recommended to enhance the understanding of tooth loss predictors in advanced periodontitis.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04217-5.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"60"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11825966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol efficacy of native <i>Metarhizium rileyi</i> (Hypocreales: Clavicipitaceae) isolates against <i>Spodoptera litura</i> (F) (Lepidoptera: Noctuidae) and in silico effect of the secondary metabolites against the virulent proteins of the insect.","authors":"P Duraimurugan, K Sankari Meena, D Neethu Roy, E Bharathi, T Meera Devi, A Chowdappa, K S V P Chandrika","doi":"10.1007/s13205-025-04226-4","DOIUrl":"10.1007/s13205-025-04226-4","url":null,"abstract":"<p><p>This study explores the insecticidal potential of secondary metabolites derived from <i>Metarhizium rileyi</i> against <i>Spodoptera litura</i> through an integrative approach involving genomics, metabolomics, and bioinformatics. Four native isolates of <i>M. rileyi</i> were identified and characterized for their insecticidal efficacy. Among these, the SlMr-DOR isolate exhibited the highest effectiveness, achieving a remarkable 90.0% mortality in laboratory bioassays and 93.3% mortality when tested with its crude extract. Gas chromatography-mass spectrometry (GC-MS) analysis of the SlMr-DOR isolate revealed a diverse profile of bioactive volatile secondary metabolites, including squalene, diethyl phthalate, 4-anilinoquinazoline, vinyl 2-ethylhexanoate, 2-phenyl-3-formyl-pyrrole, chloramphenicol, hentriacontane, phthalic acid derivatives, pentacosane, nonanamide, and eicosanoic acid. Among these metabolites, commercially available squalene and diethyl phthalate were further validated for their insecticidal activity through bioassay studies. Molecular docking analysis demonstrated the strong binding affinity of these metabolites with key <i>S. litura</i> target proteins, including putative chemosensory protein CSP8 and α-amylase, revealing their potential mode of action. The results establish that the secondary metabolites, particularly from the SlMr-DOR isolate, are highly effective against <i>S. litura</i>. This study emphasizes the potential of such metabolites as sustainable and effective alternatives for pest management strategies, contributing to integrated pest control approaches and reducing reliance on synthetic chemical pesticides.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"57"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of <i>Streptomyces rochei</i> 8ER183 for poly(lactic acid)-degrading enzyme production, biodegradative capability, and its whole-genome sequence characterization.","authors":"Kanaporn Sujarit, Butsakorn Pannim, Nattawan Kuakkhunthod, Udomlak Uywannang, Chatsuda Sakdapetsiri, Titiporn Panyachanakul, Sukhumaporn Krajangsang, Supattra Phanngoen, Vichien Kitpreechavanich, Thanasak Lomthong","doi":"10.1007/s13205-025-04219-3","DOIUrl":"10.1007/s13205-025-04219-3","url":null,"abstract":"<p><p>Ninety-eight actinomycetes isolates were screened for poly(lactic acid) (PLA)-degrading abilities using a minimal medium supplemented with emulsified PLA as the substrate. The isolate 8ER183 showed PLA degradation ability after incubation at ambient temperature (30 ± 2 °C) for 96 h. The 16S rRNA gene and whole-genome sequencing identified strain 8ER183 as <i>Streptomyces rochei</i>, and the genome size was 8.4 Mbp with an average G + C content of 72.39%. Genome mining revealed 5,689 proteins with functional assignments. The predicted degradation gene involving PLA-degrading enzymes such as protease and lipase was correlated with the phenotypic investigation. The enzymes involved in PLA degradation produced by 8ER183 strain were evaluated as protease and lipase. For enzyme production, cassava chips and peptone at 1.0 and 5.0 g/L yielded the highest PLA-degrading enzyme production (0.49 ± 0.02 U/mL) at 45 °C with pH 8.0. Scaling up enzyme production in a 3.0 L airlift bioreactor enhanced enzyme yield to 2.57 ± 0.12 U/mL, representing 5.25- and 32.12-fold increases compared to the optimized medium in shaking flasks and the unoptimized medium, respectively. The crude enzyme was most active at pH 9.0 and 50 °C. Electron microscopy and infrared spectroscopy revealed significant differences in the physical and chemical structures of PLA film after degradation by crude enzyme. This research identified and characterized a novel PLA-degrading actinomycetes strain as a solution to reduce bioplastic accumulation in the environment and contribute to a more sustainable future.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04219-3.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"55"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11802947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the microbiome dynamics in an effective banana Fusarium wilt biocontrol interaction system.","authors":"Amaresh Hadimani, Thangavelu Raman, Edwinraj Esack, M Loganathan, Deepa Jaganathan, V Kantharaju, R Selvarajan","doi":"10.1007/s13205-025-04223-7","DOIUrl":"10.1007/s13205-025-04223-7","url":null,"abstract":"<p><p>This study explored the effects of bacterial and fungal biocontrol agents (consortia) on the microbiome of Fusarium wilt (Foc TR4)-infected Cavandish banana soils in terms of alteration of prevalence and abundance. The results showed a significant shift in microbial diversity, dominance, abundance, evenness, richness and composition core and indicator microbiome in response to soil applied consortia and untreated controls. A total of 2857 bacterial OTUs from 331 families across 40 phyla dominated with Bacillaceae (40.2%), Acidobacteriaceae (14.2%), Haloarculaceae (12.6%), and Paenibacillaceae (9.4%). There were 4,868 fungal OTUs from 520 families across 18 phyla dominant with Mortierellaceae (20.9%), Cortinariaceae (7.6%), Aspergillaceae (6.2%), Pandeidae (5.6%), and Pyronemataceae (5.0%). Alpha diversity analysis indicated that bacterial diversity varied across treatments where T2 has the highest OTUs, while fungal diversity remained relatively stable across the treatments. Beta diversity and PCoA analysis revealed the differences in community compositions across treatments in both bacterial and fungal microbiome. Bacterial communities in T3 and T5 were highly similar, whereas T4 had a notable difference in fungal communities. This study identified a total of 192 bacterial core OTUs dominated with Firmicutes, Proteobacteria, and Acidobacteriia. In the case of fungi, 59 core OTUs from Ascomycota, Basidiomycota, and Mucoromycota are the most abundant ones within the treatments. Venn diagram revealed unique, common and shared OTUs suggesting antagonistic interactions of the soil applied consortia. DESeq2 analysis revealed a significant shift of core microbiome, where positive fold changes in Betaproteobacteria for bacterial, and <i>Fusarium</i> sp. for fungi were noticeable. Heatmap analysis revealed the treatment-dependent differences in community composition where T2 has higher bacterial abundance and T4 has higher fungal abundance suggesting that the biocontrol treatments affect the soil microbiome differently depending on the combinations and the origins of the consortia. The indicator species analysis identified 37 bacterial and 34 fungal OTUs that were specific and indicative of particular treatments that suggest microbial consortia might be selectively enhancing the growth of functionally beneficial microbial populations of the soil that promote soil health and disease suppressiveness. This study recommends that the use of biocontrol agents in the form of consortia would not only expand the diversity of the soil microbiome but also improve the effectiveness and the sustainability of Fusarium wilt management.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04223-7.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"59"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11822171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring therapeutic potential of <i>Bacopa monnieri</i> bioactive compounds against Alzheimer's and Parkinson's diseases.","authors":"Richa Shukla, Krishna Mishra, Sangeeta Singh","doi":"10.1007/s13205-025-04224-6","DOIUrl":"10.1007/s13205-025-04224-6","url":null,"abstract":"<p><p>Parkinson's disease (PD) and Alzheimer's disease (AD) consist of progressive illnesses of central nervous system that primarily affect the elderly and are characterized by movement symptoms, memory decline, and cognitive impairment. A number of variables, including the lack of a novel treatment, a steady rise in the patient population, and the high expense of care and treatment, have contributed to the growing significance of these diseases. In recent decades, we have gained a better understanding of the causes of diseases, but complex mechanisms of neuronal loss, combined with physiological factors that are incompatible, pose challenges in describing the pathogenic processes and devising effective treatments. Currently, there are no known treatments for most of these diseases, rendering them incurable. Therefore, there is a pressing need for therapeutic interventions that have the potential to effectively treat neurodegeneration. This study aimed to evaluate the efficacy of the ayurvedic herb <i>Bacopa monnieri</i> bioactive components against the therapeutic targets HTR1A, HTR1B, HTR2A, HTR2C, HTR7, alpha-synuclein, amyloid beta, and tau protein of Alzheimer's and Parkinson's illnesses. The docking analysis revealed the promising binding affinity with Quercetin, Apigenin, and Luteolin and Molecular mechanics/generalized Born surface area (MM/GBSA) further confirmed the stability of the complexes. In vitro investigation indicated that Quercetin is the most effective for treating AD and PD due to its considerable inhibition of alpha-synuclein production, whereas Luteolin is the favorable one for preventing both diseases by mitigating effects during Rotenone treatment. The future implications and constraints of the current study suggest that further validation in Invivo models of Alzheimer's and Parkinson's diseases is necessary to investigate the effects of Quercetin and Apigenin in the treatment of these conditions, as well as Luteolin and Quercetin for their prevention.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04224-6.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"61"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11828772/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}