IF 2.6 4区生物学

3 Biotech Pub Date : 2024-10-01 Epub Date: 2024-09-05 DOI: 10.1007/s13205-024-04066-8

Siddhartha Pal, Arpita Hait, Sunanda Mandal, Ajoy Roy, Pinaki Sar, Sufia K Kazy

{"title":"Crude oil degrading efficiency of formulated consortium of bacterial strains isolated from petroleum-contaminated sludge.","authors":"Siddhartha Pal, Arpita Hait, Sunanda Mandal, Ajoy Roy, Pinaki Sar, Sufia K Kazy","doi":"10.1007/s13205-024-04066-8","DOIUrl":"https://doi.org/10.1007/s13205-024-04066-8","url":null,"abstract":"Crude oil contamination has been widely recognized as a major environmental issue due to its various adverse effects. The use of inhabitant microorganisms (native to the contaminated sites) to detoxify/remove pollutants owing to their diverse metabolic capabilities is an evolving method for the removal/degradation of petroleum industry contaminants. The present study deals with the exploitation of native resident bacteria from crude oil contaminated site (oil exploration field) for bioremediation procedures. Fifteen (out of forty-four) bioremediation-relevant aerobic bacterial strains, belonging to the genera of Bacillus, Stenotrophomonas, Pseudomonas, Paenibacillus, Rhizobium, Burkholderia, and Franconibacter, isolated from crude oil containing sludge, have been selected for the present bioremediation study. Crude oil bioremediation performance of the selected bacterial consortium was assessed using microcosm-based studies. Stimulation of the microbial consortium with nitrogen or phosphorous led to the degradation of 60-70% of total petroleum hydrocarbon (TPH) in 0.25% and 0.5% crude oil experimental sets. CO2 evolution, indicative of crude oil mineralization, was evident with the highest evolution being 28.6 mg mL-1. Ecotoxicity of treated crude oil-containing media was assessed using plant seed germination assay, in which most of the 0.25% and 0.5% treated crude oil sets gave positive results thereby suggesting a reduction in crude oil toxicity.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11377402/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Duckweeds as edible vaccines in the animal farming industry. 鸭茅作为动物养殖业的食用疫苗。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-10-01 Epub Date: 2024-09-06 DOI: 10.1007/s13205-024-04074-8

Anca Awal Sembada, Yohanes Theda, Ahmad Faizal

{"title":"Duckweeds as edible vaccines in the animal farming industry.","authors":"Anca Awal Sembada, Yohanes Theda, Ahmad Faizal","doi":"10.1007/s13205-024-04074-8","DOIUrl":"https://doi.org/10.1007/s13205-024-04074-8","url":null,"abstract":"Animal diseases are among the most debilitating issues in the animal farming industry, resulting in decreased productivity and product quality worldwide. An emerging alternative to conventional injectable vaccines is edible vaccines, which promise increased delivery efficiency while maintaining vaccine effectiveness. One of the most promising platforms for edible vaccines is duckweeds, due to their high growth rate, ease of transformation, and excellent nutritional content. This review explores the potential, feasibility, and advantages of using duckweeds as platforms for edible vaccines. Duckweeds have proven to be superb feed sources, as evidenced by numerous improvements in both quantity (e.g., weight gain) and quality (e.g., yolk pigmentation). In terms of heterologous protein production, duckweeds, being plants, are capable of expressing proteins with complex structures and post-translational modifications. Research efforts have focused on the development of duckweed-based edible vaccines, including those against avian influenza, tuberculosis, Newcastle disease, and mastitis, among others. As with any emerging technology, the development of duckweeds as a platform for edible vaccines is still in its early stages compared to well-established injectable vaccines. It is evident that more proof-of-concept studies are required to bring edible vaccines closer to the current standards of conventional vaccines. Specifically, the duckweed expression system needs further development in areas such as yield and growth rate, especially when compared to bacterial and mammalian expression systems. Continued efforts in this field could lead to breakthroughs that significantly improve the resilience of the animal farming industry against disease threats.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11379843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Developing a novel neutralizing monoclonal antibody against TrkB. 开发针对 TrkB 的新型中和单克隆抗体。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-10-01 Epub Date: 2024-09-05 DOI: 10.1007/s13205-024-04063-x

Gamze Eda Yildirim, Erkan Yilmaz

{"title":"Developing a novel neutralizing monoclonal antibody against TrkB.","authors":"Gamze Eda Yildirim, Erkan Yilmaz","doi":"10.1007/s13205-024-04063-x","DOIUrl":"https://doi.org/10.1007/s13205-024-04063-x","url":null,"abstract":"The TrkB receptor, which is highly expressed in various human cancers and considered a pro-oncogene, was targeted to develop neutralizing monoclonal antibodies against its immunoglobulin-like (Ig-like) domains. Recombinant TrkB-IgL peptide, including the Ig-like C2 type 1 (Ig-C2-type 1) and Ig-like C2 type 2 (Ig-C2-type 2) domains, was expressed and purified from E. coli. Mice were immunized with this peptide, and hybridoma clones producing anti-TrkB-IgL antibodies were generated. Among 23 ELISA-positive TrkB-IgL hybridoma clones, four (TrkB-IgL 5.11, 4.11, 4.6, 4.3) showed anti-proliferative effects compared to the control on human breast cancer (MCF-7) and human colon cancer (HCT116) cells, as assessed using the xCELLigence system. Western blot analysis revealed that TrkB-IgL 5.11 and 4.11 significantly suppressed TrkB-mediated signaling pathways compared to the control. Purified TrkB-IgL monoclonal antibodies (mAbs) exhibited anti-proliferative effects compared to both positive and negative controls using the xCELLigence system. The TrkB-IgL 5.11 mAb notably suppressed phosphorylation of TrkB, Akt, and ERK and induced Caspase-3 and Caspase-9 activities in a dose-dependent manner, as determined by Western blotting. Additionally, immunostaining confirmed the localization of these mAbs on the SH-SY5Y cell membrane, which is known for high TrkB expression. In conclusion, the TrkB-IgL 5.11 antibody effectively inhibits cancer cell proliferation and induces apoptosis by suppressing key signaling pathways. These findings demonstrate the potential of this antibody as a therapeutic agent for cancers that overexpress TrkB. Additionally, it is considered a promising candidate for humanization, which would facilitate its application in cancer treatment.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04063-x.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11377376/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of chronic administration of oxycodone on the behavioral functions and histopathology in the cerebellum and striatum of adult male rats. 长期服用羟考酮对成年雄性大鼠小脑和纹状体行为功能和组织病理学的影响

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-10-01 Epub Date: 2024-09-06 DOI: 10.1007/s13205-024-04062-y

Farzin Banei, Abbas Aliaghaei, Gholam Hossein Meftahi

{"title":"The effect of chronic administration of oxycodone on the behavioral functions and histopathology in the cerebellum and striatum of adult male rats.","authors":"Farzin Banei, Abbas Aliaghaei, Gholam Hossein Meftahi","doi":"10.1007/s13205-024-04062-y","DOIUrl":"https://doi.org/10.1007/s13205-024-04062-y","url":null,"abstract":"Oxycodone is widely used for pain management and acts via binding to mu- and kappa opioid receptors. It was shown that extended oxycodone usage can result from the demyelination and degeneration of neurons through the stress response, which triggers apoptotic signaling pathways. The striatum and cerebellum are recognized as significant contributors to addiction; however, there is no report on the effect of oxycodone on the cerebellum and striatum and motor coordination. We treated rats daily with oxycodone at 15 mg/kg doses for thirty days. Motor performance and electromyography activity were then evaluated. Stereological methods were performed to assess the number of neurons in the cerebellum and striatum as well as immunohistochemistry for microgliosis and astrogliosis. Furthermore, the Sholl analysis method was utilized to evaluate the cellular structure of both microglia and astrocytes. Results of the rotarod test for motor coordination show no significant (P < 0.05) difference between the oxycodone subjects and those in the control group. In addition, open-field assessments indicated that the application of oxycodone did not alter the amount of distance covered (as an indicator of locomotion) or time spent in the central area (as an indicator of anxiety) (P < 0.001). The electromyography (EMG) test result showed that oxycodone caused a delay in the reaction of the muscular nerves (P < 0.001). Data and results from our experiment revealed that administering oxycodone did not affect astrogliosis and the number of neurons in the cerebellum and striatum (P < 0.05). In contrast, it altered neuromuscular function. In addition, oxycodone administration activated microglia in the cerebellum and striatum. In conclusion, we encourage more research on the adverse effects of oxycodone on the brain.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11379841/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Revitalizing common drugs for antibacterial, quorum quenching, and antivirulence potential against Pseudomonas aeruginosa: in vitro and in silico insights. 唤醒普通药物对铜绿假单胞菌的抗菌、淬灭法定量和抗病毒潜力：体外和硅学见解。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-10-01 Epub Date: 2024-09-04 DOI: 10.1007/s13205-024-04070-y

Jatin Chadha, Umang Mudgil, Lavanya Khullar, Prerna Ahuja, Kusum Harjai

{"title":"Revitalizing common drugs for antibacterial, quorum quenching, and antivirulence potential against Pseudomonas aeruginosa: in vitro and in silico insights.","authors":"Jatin Chadha, Umang Mudgil, Lavanya Khullar, Prerna Ahuja, Kusum Harjai","doi":"10.1007/s13205-024-04070-y","DOIUrl":"https://doi.org/10.1007/s13205-024-04070-y","url":null,"abstract":"In the post-antibiotic era, antivirulence therapies are becoming refractory to the clinical application of existing antimicrobial regimens. Moreover, in an attempt to explore alternate intervention strategies, drug repurposing is gaining attention over development of novel drugs/antimicrobials. With the prevalence of multidrug resistance and high medical burden associated with Pseudomonas aeruginosa, there is an urgent need to devise novel therapeutics to combat this bacterial pathogen. In this context, the present study was undertaken to scrutinize the anti-quorum sensing (QS) and antivirulence potential of commonly consumed drugs such as fexofenadine (FeX), ivermectin (IvM), nitrofurantoin (NiT), levocetrizine (LvC), atorvastatin (AtS), and aceclofenac (AcF), against P. aeruginosa. The methodology involved assessment of antibacterial activity against P. aeruginosa PAO1 and quorum quenching (QQ) potential using Agrobacterium tumefaciens NTL4 biosensor strain. The antivirulence prospects were investigated by estimating the production of hallmark virulence factors in P. aeruginosa accompanied by molecular docking to predict drug associations with the QS receptors. Interestingly, all the drugs harbored antibacterial, anti-QS, and antivirulence potential in vitro, which consequently disrupted QS circuits and attenuated pseudomonal virulence phenotypically by significantly lowering the production of pyocyanin, hemolysin, pyochelin, and total bacterial protease in vitro. Moreover, the findings were validated by computational studies that predicted strong molecular interactions between the test drugs and QS receptors of P. aeruginosa. Hence, this study is the first to suggest the prospect of repurposing FeX, IvM, NiT, LvC, AtS, and AcF against P. aeruginosa.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371971/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142138973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alternaria alternata species complex impairing solanaceous vegetables in Northern parts of India: an emerging problem in Solanum lycopersicum L. 影响印度北部地区茄科蔬菜的交替孢属复合菌：番茄茄属植物新出现的问题

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-10-01 Epub Date: 2024-09-02 DOI: 10.1007/s13205-024-04059-7

Rekha Balodi, Kerur Vishwanath Raghavendra, Praveen Kumar Singh, Zakir Hussain, Sachin Suresh Suroshe, Pawan Kumar, Subhash Chander

{"title":"Alternaria alternata species complex impairing solanaceous vegetables in Northern parts of India: an emerging problem in Solanum lycopersicum L.","authors":"Rekha Balodi, Kerur Vishwanath Raghavendra, Praveen Kumar Singh, Zakir Hussain, Sachin Suresh Suroshe, Pawan Kumar, Subhash Chander","doi":"10.1007/s13205-024-04059-7","DOIUrl":"10.1007/s13205-024-04059-7","url":null,"abstract":"Severe incidence of Alternaria spp. was observed in the three solanaceous vegetables viz. tomato, potato and brinjal grown in National Capital Region of Delhi and Haryana. The symptomatic plants exhibited necrotic brown lesions on the leaves initiating from the lower older leaves and extending to stem, affecting the whole plant. Subsequently, black sooty sporulation was observed in fruits of tomato and brinjal crop. During the survey, tomato crop was most susceptible with 98.99% incidence, followed by brinjal with 82.36% and potato with 61.19% incidence. The mean plant disease index varied between 9.98% and 65.99% among the three crops. Isolation and characterization of the causal agent from symptomatic plants revealed association of small-spored Alternaria spp. in all crops. Further, morphological and Internal Transcribed Spacer (ITS) sequencing phylogenetic analysis ascertained the fungus belong to Alternaria section of Alternaria genus. As tomato was found most susceptible, host plant resistance was explored among 197 tomato germplasm/breeding lines under epiphytotic conditions. It was found that 08 accessions exhibited moderate resistance against pathogen. The findings in the study suggest that small-spored Alternaria is an emerging problem in the solanaceous vegetables and warrants attention for effective management of the disease.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04059-7.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11368882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142131480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring microbial diversity in the rhizosphere: a comprehensive review of metagenomic approaches and their applications. 探索根圈微生物多样性：元基因组学方法及其应用综述。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-10-01 Epub Date: 2024-09-06 DOI: 10.1007/s13205-024-04065-9

Bhumi Rajguru, Manju Shri, Vaibhav D Bhatt

{"title":"Exploring microbial diversity in the rhizosphere: a comprehensive review of metagenomic approaches and their applications.","authors":"Bhumi Rajguru, Manju Shri, Vaibhav D Bhatt","doi":"10.1007/s13205-024-04065-9","DOIUrl":"https://doi.org/10.1007/s13205-024-04065-9","url":null,"abstract":"The rhizosphere, the soil region influenced by plant roots, represents a dynamic microenvironment where intricate interactions between plants and microorganisms shape soil health, nutrient cycling, and plant growth. Soil microorganisms are integral players in the transformation of materials, the dynamics of energy flows, and the intricate cycles of biogeochemistry. Considerable research has been dedicated to investigating the abundance, diversity, and intricacies of interactions among different microbes, as well as the relationships between plants and microbes present in the rhizosphere. Metagenomics, a powerful suite of techniques, has emerged as a transformative tool for dissecting the genetic repertoire of complex microbial communities inhabiting the rhizosphere. The review systematically navigates through various metagenomic approaches, ranging from shotgun metagenomics, enabling unbiased analysis of entire microbial genomes, to targeted sequencing of the 16S rRNA gene for taxonomic profiling. Each approach's strengths and limitations are critically evaluated, providing researchers with a nuanced understanding of their applicability in different research contexts. A central focus of the review lies in the practical applications of rhizosphere metagenomics in various fields including agriculture. By decoding the genomic content of rhizospheric microbes, researchers gain insights into their functional roles in nutrient acquisition, disease suppression, and overall plant health. The review also addresses the broader implications of metagenomic studies in advancing our understanding of microbial diversity and community dynamics in the rhizosphere. It serves as a comprehensive guide for researchers, agronomists, and policymakers, offering a roadmap for harnessing metagenomic approaches to unlock the full potential of the rhizosphere microbiome in promoting sustainable agriculture.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11379838/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Green synthesis of chitosan nanoparticles using Cassia fistula leaf extract: evaluation of antimicrobial, antioxidant, antibiofilm, and cytotoxic activities. 利用决明子叶提取物绿色合成壳聚糖纳米颗粒：抗菌、抗氧化、抗生物膜和细胞毒性活性评估。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-10-01 Epub Date: 2024-09-06 DOI: 10.1007/s13205-024-04073-9

Anusiya Ganesan, Jaiganesh Rengarajan

{"title":"Green synthesis of chitosan nanoparticles using Cassia fistula leaf extract: evaluation of antimicrobial, antioxidant, antibiofilm, and cytotoxic activities.","authors":"Anusiya Ganesan, Jaiganesh Rengarajan","doi":"10.1007/s13205-024-04073-9","DOIUrl":"https://doi.org/10.1007/s13205-024-04073-9","url":null,"abstract":"The emerging field of green synthesis within nanobiotechnology presents significant environmental and economic advantages compared to conventional methodologies. This study investigates the synthesis and application of chitosan nanoparticles (ChNPs) using Cassia fistula (CF) leaf extract as a sustainable, and bio-based approach. Characterization of CF-ChNPs confirmed effective bioconversion and also demonstrated significant antimicrobial activity. Notably, CF-ChNPs demonstrated a remarkable antimicrobial effect against Pseudomonas aeruginosa, with a zone of inhibition of 17 ± 0.2 mm surpassing the impact on other organisms tested. The CF-ChNPs exhibited an initial burst release of 28 ± 0.28% after 2 h, gradually achieving a controlled release of 76.3 ± 0.43% within 24 h. In addition, CF-ChNPs exhibited an antioxidant activity of 43.1 ± 0.48% and showed excellent antibiofilm activity against Staphylococcus aureus in comparison to other organisms. The cell viability assay results have confirmed that CF-ChNPs do not have any negative impact on the viability of L929 fibroblasts, further highlighting their potential as versatile nanomaterials for treating microbial infections and other therapeutic applications.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11379835/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142152958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prediction of molecular targets for antidepressant potential of hydroalcoholic extract of Tamarindus indica using network pharmacology approach and evaluating its efficacy in Chronic Unpredictable Mild Stress model in mice 利用网络药理学方法预测罗望子水醇提取物抗抑郁潜力的分子靶点，并评估其在小鼠慢性不可预测轻度应激模型中的疗效

IF 2.8 4区生物学

3 Biotech Pub Date : 2024-09-13 DOI: 10.1007/s13205-024-04081-9

Vishnusai Beere, Khushboo Choudhary, Priya Bisht, Amita Rai, Nitesh Kumar

{"title":"Prediction of molecular targets for antidepressant potential of hydroalcoholic extract of Tamarindus indica using network pharmacology approach and evaluating its efficacy in Chronic Unpredictable Mild Stress model in mice","authors":"Vishnusai Beere, Khushboo Choudhary, Priya Bisht, Amita Rai, Nitesh Kumar","doi":"10.1007/s13205-024-04081-9","DOIUrl":"https://doi.org/10.1007/s13205-024-04081-9","url":null,"abstract":"The prevalence of psychological disorders has surged since the 1990s, posing a significant global health burden with depressed individuals averaging six lost hours per week and contributing to over 20% of all missed workdays. Current antidepressants, while effective for some, have limited efficacy, dietary restrictions, and adverse effects, including liver damage and hypertension. Natural remedies offer promising therapeutic potential with minimal side effects. Tamarindus indica (TI) is a plant that grows in the shape of a tree. Network pharmacology of TI revealed the key targets MAPK, D1-6, 5HT, DAT, MAO, COMT, PKA, PKC, AKT, and VMAT, which are linked to prominent key pathways such as dopaminergic and serotonergic. The cell viability assays on SH-Sy5y cells indicated a favourable safety profile with an IC50 of 573.99 µg/ml and further, the in vivo efficacy was observed through Chronic Unpredictable Mild Stress (CUMS) model in mice. The hydroalcoholic extract of TI demonstrated antidepressant effects, significantly reducing immobility time in the Tail Suspension Test (TST) and Forced Swim Test (FST). Additionally, locomotor activity, assessed via the Open Field Test (OFT), was significantly increased in the treatment group compared to CUMS mice. Biochemical analyses revealed elevated Brain Derived Neurotropic Factor (BDNF), decreased cortisol levels, and reduced catechol-O-methyltransferase (COMT) concentration in TI-treated (50 mg/kg) groups. These findings underscore the potential of TI as a natural antidepressant, offering a promising avenue for further therapeutic development in depression management. The current study did not evaluate the level of neurotransmitters in the brain, which will be evaluated in future studies.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The structural insights of L-asparaginase from Pseudomonas aeruginosa CSPS4 at elevated temperatures highlight its thermophilic nature 铜绿假单胞菌 CSPS4 的 L-天冬酰胺酶在高温下的结构研究凸显其嗜热特性

IF 2.8 4区生物学

3 Biotech Pub Date : 2024-09-11 DOI: 10.1007/s13205-024-04072-w

Vinay Kumar, Pragya Anand, Ankita Srivastava, Yusuf Akhter, Digvijay Verma

{"title":"The structural insights of L-asparaginase from Pseudomonas aeruginosa CSPS4 at elevated temperatures highlight its thermophilic nature","authors":"Vinay Kumar, Pragya Anand, Ankita Srivastava, Yusuf Akhter, Digvijay Verma","doi":"10.1007/s13205-024-04072-w","DOIUrl":"https://doi.org/10.1007/s13205-024-04072-w","url":null,"abstract":"In the present investigation, a novel thermophilic L-asparaginase (Asn_PA) from Pseudomonas aeruginosa CSPS4 was investigated to explore its structural insights at elevated temperatures. Sequence analysis of Asn_PA depicted three conserved motifs (VVILATGGTIAG, DGIVITHGTDTLEETAYFL, and, LRKQGVQIIRSSHVNAGGF), of them, two motifs exhibit catalytically-important residues i.e., T45 and T125. A homology modelling-based structure model for Asn_PA was generated with 4PGA as the top-matched template. The predicted structure was validated and energy was minimized. Molecular docking was carried out cantered at the active site for asparagine and glutamine as its substrate ligands. The enzyme–substrate interaction analysis showed binding affinities of – 4.8 and – 4.1 kcal/mol for asparagine and glutamine respectively. Molecular dynamics (MD) simulation studies showed a better stability of Asn_PA at temperatures of 60 °C, over 40, 50 and, 80 °C, making this enzyme a novel L-asparaginase from other mesophilic P. aeruginosa strain. The trajectory analysis showed that RMSD, Rg, and, SASA values correlate well with each other in the different tested temperatures during the MD analysis. Thus, the present findings encourage extensive characterization of the Asn_PA using laboratory experiments to understand the structural behavior of the active site loop in an open or closed state with and without the substrate molecules.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142182841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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