IF 2.6 4区生物学

3 Biotech Pub Date : 2025-06-01 Epub Date: 2025-05-15 DOI: 10.1007/s13205-025-04316-3

Yanhong Yu, Hailing Lv, Xiaoyu Liu, Aiju Liu

{"title":"Exploring the therapeutic efficacy of Bai-Shao in mitigating comorbid epileptic seizures and cognitive impairment via inflammatory signaling pathways: insights from in silico and in vivo studies.","authors":"Yanhong Yu, Hailing Lv, Xiaoyu Liu, Aiju Liu","doi":"10.1007/s13205-025-04316-3","DOIUrl":"10.1007/s13205-025-04316-3","url":null,"abstract":"Bai-Shao (BS) ingredients capable of crossing the blood-brain barrier (BBB) were identified and analyzed for their main gene targets. Genes associated with epileptic seizures (ES), cognitive impairment (CI), and inflammation were retrieved, with common targets between BS and these conditions determined via Venn analysis. Protein-protein interaction (PPI) analysis identified the top 10 key genes, whose correlations with apolipoprotein E (APOE) and methylenetetrahydrofolate reductase (MTHFR) were assessed. Functional pathways were explored using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG), while molecular docking evaluated interactions between BS compounds and target proteins. Findings were validated in a pentylenetetrazol (PTZ)-induced mouse model. BS contains β-sitosterol and betulinic acid, both BBB-permeable, with 277 shared ES/CI-related targets identified. The top 10 genes correlated with APOE and MTHFR, and pathway analysis highlighted interleukin-17 (IL-17) and tumor necrosis factor (TNF) signaling, involving FOS proto-oncogene (FOS), jun proto-oncogene (JUN), caspase-3 (CASP3), phosphoinositide 3-kinase (PI3K), protein kinase B (AKT), and cyclooxygenase-2 (COX2). Behavioral tests, including the Open Field Test and Morris Water Maze, indicated cognitive and motor improvements in PTZ-treated mice. Molecular analysis revealed hippocampal regulation of key genes and altered TNF-alpha (TNFα), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) levels. In conclusion, BS demonstrates therapeutic potential for comorbid ES and CI by modulating inflammatory pathways and cell survival mechanisms, supporting its role in neurological research.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04316-3.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 6","pages":"171"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic activity and pathway study of emerging contaminants biodegradation using a photo-bioelectrochemical system: a review. 光-生物电化学系统降解新污染物的代谢活性及途径研究综述。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-06-01 Epub Date: 2025-05-16 DOI: 10.1007/s13205-025-04340-3

Zohreh Moghiseh, Farnaz Almasi

{"title":"Metabolic activity and pathway study of emerging contaminants biodegradation using a photo-bioelectrochemical system: a review.","authors":"Zohreh Moghiseh, Farnaz Almasi","doi":"10.1007/s13205-025-04340-3","DOIUrl":"10.1007/s13205-025-04340-3","url":null,"abstract":"Emerging contaminants and their intermediate residue pose significant risks to human health and ecosystems due to their persistence in water and soil. The review explores the potential of photo-bioelectrochemical systems as a dual-function approach for the degradation of emerging contaminants while simultaneously generating bioenergy. Photo-bioelectrochemical systems integrate microbial metabolism with photocatalytic processes, utilizing solar energy to enhance electron transfer and promote the breakdown of toxic compounds. Recently, this technology has demonstrated improved removal rates of various emerging contaminants, including antibiotics and neonicotinoid pesticides, while producing valuable by-products such as hydrogen and methane through optimized operational conditions. Although studies have focused mainly on pharmaceuticals and dyes, applying these systems for pesticides remains underexplored. Algae-assisted photo-bioelectrochemical systems have been observed frequently in recent studies. This review synthesizes current research on the metabolic activity and biochemical pathways involved in the biodegradation of contaminants within photo-bioelectrochemical systems. It highlights the efficiency of these systems in converting harmful substances into less toxic by-products. It addresses challenges such as slow degradation rates compared to chemical methods and the need for enhanced extracellular electron transfer capabilities among microorganisms. Through optimized operational conditions, this paper aims to provide a comprehensive understanding of photo-bioelectrochemical systems' performance and to propose future research directions for optimizing these systems in real-world applications. In conclusion, this work underscores the promise of photo-bioelectrochemical systems as a sustainable solution for wastewater treatment and environmental remediation.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 6","pages":"173"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12084208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-silico molecular investigation of Nannochloropsis microalgae cellulose synthase under salinity conditions and in-vitro evaluation of the proportionate effects on cellulose production. 盐度条件下纳米叶绿体微藻纤维素合酶的硅晶分子研究及对纤维素产量的比例效应体外评价。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-06-01 Epub Date: 2025-05-21 DOI: 10.1007/s13205-025-04329-y

Seyed Mohammad Hasan Haghayeghi, Maryam Azimzadeh Irani, Hossein Askari, Madihe Sadat Rasa, Zeinab Shariatmadari

{"title":"In-silico molecular investigation of Nannochloropsis microalgae cellulose synthase under salinity conditions and in-vitro evaluation of the proportionate effects on cellulose production.","authors":"Seyed Mohammad Hasan Haghayeghi, Maryam Azimzadeh Irani, Hossein Askari, Madihe Sadat Rasa, Zeinab Shariatmadari","doi":"10.1007/s13205-025-04329-y","DOIUrl":"10.1007/s13205-025-04329-y","url":null,"abstract":"Nannochloropsis is a microalgae with more than substantially 60-70% cellulose in its cell wall, making it a potential candidate for nanocellulose sustainable production. This study examined the effects of salts in seawater and their role on Nannochloropsis gaditana and Nannochloropsis oculata cellulose synthase activity using In-silico and In-vitro approaches for the first time. Deep-learning-based AlphaFold2 predicted model was selected as the most reliable 3D structure. Molecular docking results revealed that none of the selected ligands occupied the binding site predicted for the native substrate of the enzyme, uridine-diphosphate. To validate the In-silico results, experiments were conducted to investigate the impact of salinity stress (NaCl, NaNO3 and NaHCO3) on the cell growth and cellulose production. The assessment tools included a UV-visible spectrophotometer and a hemocytometer, with a modified Jayme-Wise method used for cellulose extraction. The results indicated that the following concentrations of 0.443 mol/L, 0.457 mol/L, and 0.469 mol/L of NaCl, 0.072 mol/L, 0.077 mol/L, and 0.082 mol/L of NaNO3, 0.0021 mol/L, 0.0022 mol/L, and 0.0023 mol/L of NaHCO3 did not lower the growth rate nor the cellulose yield of N. oculata and notable enhancement in growth was observed in cultures supplemented with 0.0023 mol/L NaHCO3. Furthermore, when NaCl (0.457 mol/L and 0.469 mol/L), NaNO3 (0.082 mol/L) and NaHCO3 (0.0022 mol/L and 0.0023 mol/L) were individually introduced to the culture, cellulose yield increased up to five times compared to the control group.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04329-y.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 6","pages":"180"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cleavage efficiency of CRISPR/Cas9 system with G-quadruplex-capped single-guide RNA motifs in RNase II and RNase R. RNase II和RNase R中含g -四重体单导RNA基序的CRISPR/Cas9系统的切割效率

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-06-01 Epub Date: 2025-05-26 DOI: 10.1007/s13205-025-04354-x

Dung Thanh Dang

{"title":"Cleavage efficiency of CRISPR/Cas9 system with G-quadruplex-capped single-guide RNA motifs in RNase II and RNase R.","authors":"Dung Thanh Dang","doi":"10.1007/s13205-025-04354-x","DOIUrl":"10.1007/s13205-025-04354-x","url":null,"abstract":"Modification of sgRNA has been considered as a necessary approach to enhance the stability and cleavage efficiency of the CRISPR/Cas9 system. In this study, a rigid G-quadruplex structure was genetically applied to the 3' end of typical sgRNA for protection of RNA from 3'-5' exoribonuclease degradation. The in vitro transcriptional production yields of sgRNAs bearing G-quadruplex structure such as sgRNA3 and sgRNA4 were around 1.4 and 1.5 times higher than the yield of typical sgRNA1, respectively. The results have also shown that appending G-quadruplex motif at the 3' end of typical sgRNAs did minorly affect the cleavage activity of CRISPR/Cas9. Interestingly, cleavage efficiency of CRISPR/Cas9 system with sgRNAs bearing the rigid G-quadruplex was fully retained in the presence of 3'-5' exoribonucleases such as RNase II or RNase R. In contrast, the cleavage activity of CRISPR/Cas9 system with the typical sgRNA1 was significantly decreased in the same condition. This protection of sgRNA through G-quadruplex structure-based modifications might provide a potential approach for improving cleavage efficiency of CRISPR/Cas9 system in the exoribonuclease environment.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04354-x.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 6","pages":"189"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12106176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144172329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

De novo assembly, gene annotation and marker development using illumina paired-end transcriptome sequences in nutmeg (Myristica fragrans Houtt.). 利用illumina配对末端转录组序列在肉豆蔻（Myristica fragrans Houtt.）中重新组装、基因注释和标记开发。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-06-01 Epub Date: 2025-05-07 DOI: 10.1007/s13205-025-04321-6

Otih Rostiana, Rubi Heryanto, Tias Arlianti, Agus Ruhnayat, Tri Puji Priyatno, Muhammad Rifqi Hariri

{"title":"De novo assembly, gene annotation and marker development using illumina paired-end transcriptome sequences in nutmeg (Myristica fragrans Houtt.).","authors":"Otih Rostiana, Rubi Heryanto, Tias Arlianti, Agus Ruhnayat, Tri Puji Priyatno, Muhammad Rifqi Hariri","doi":"10.1007/s13205-025-04321-6","DOIUrl":"https://doi.org/10.1007/s13205-025-04321-6","url":null,"abstract":"Nutmeg (Myristica fragrans) is a high-value spice crop widely utilized in culinary, medicinal, and industrial sectors. Despite its economic and cultural significance, genetic research on nutmeg is severely limited, hindering efforts to improve its cultivation and breeding programs. The lack of molecular markers and genetic tools has further constrained the development of superior trait varieties. Molecular markers are essential for genetic mapping, diversity studies, and marker-assisted selection, yet their application in nutmeg remains unexplored. This study utilized Illumina high-throughput paired-end transcriptome sequencing to explore the genetic framework of nutmeg, focusing on de novo assembly, gene annotation, and molecular marker development. RNA Sequencing (RNA-seq) was performed on leaf tissues from male and female trees to analyze differentially expressed genes. A total of 141,192 unigenes were assembled with an average length of 1117 bp, achieving functional annotation in public databases like NR, Swiss-Prot, KEGG, and InterPro. De novo transcriptome assembly and functional annotation revealed ten candidate genes involved in sex determination. These candidate genes are associated with hormonal pathways, transcription factors, and reproductive development. Several primers/markers have been designed and validated using PCR. This research lays a foundation for genetic studies, marker-assisted breeding, and improved cultivation practices for nutmeg.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 6","pages":"157"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12058621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling probiotic and prebiotic functional dairy foods: a health beneficial outlook. 揭示益生菌和益生元功能乳制品：健康有益的前景。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-06-01 Epub Date: 2025-05-17 DOI: 10.1007/s13205-025-04341-2

Rajashree Jena, Prasanta Kumar Choudhury

{"title":"Unveiling probiotic and prebiotic functional dairy foods: a health beneficial outlook.","authors":"Rajashree Jena, Prasanta Kumar Choudhury","doi":"10.1007/s13205-025-04341-2","DOIUrl":"10.1007/s13205-025-04341-2","url":null,"abstract":"Functional dairy foods represent a rapidly growing category in the food industry, driven by enormous consumer demand for health promotion and disease prevention. The interplay between probiotics and prebiotics in these products has the potential to enhance their overall health supports. Probiotics, such as Lactobacillus and Bifidobacterium species, commonly found in fermented dairy foods like yogurt, fermented milk, and whey beverages, support gut health by improving the intestinal homeostasis, that aids digestion, nutrient absorption, and strengthen the immune functionality. Meanwhile, prebiotics, naturally present in foods like chicory root, garlic, onions, and certain whole grains, serve as a nutritional source that promotes the growth of these probiotics. Synbiotics, the combined intake of probiotics and prebiotics, in functional dairy foods creates a synergistic effect, improving the survival and colonization of probiotics in the gut while amplifying their positive effects. It can trigger digestive health, reduce inflammation, and boost host immunity. Additionally, scientific research suggests that synbiotics offer benefits beyond gut health, including improved respiratory health and potential impacts on mental well-being through the gut-brain axis. Dairy products fortified with synbiotics provide a convenient and appealing way to incorporate these health-promoting components into the diet, making them increasingly popular in functional foods. Understanding the interactions between them, as well as the mechanisms behind their synergism, opens the door for developing innovative dairy products with enhanced nutraceutical potential.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 6","pages":"175"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12084483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of AKTIP as a biomarker for fibrolamellar carcinoma using WGCNA and machine learning. 利用WGCNA和机器学习鉴定AKTIP作为纤维板层癌的生物标志物。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-06-01 Epub Date: 2025-05-21 DOI: 10.1007/s13205-025-04323-4

Rui Wang, Bo Ren, Xijie Zhang, Bo Liu, Wence Zhou

{"title":"Identification of AKTIP as a biomarker for fibrolamellar carcinoma using WGCNA and machine learning.","authors":"Rui Wang, Bo Ren, Xijie Zhang, Bo Liu, Wence Zhou","doi":"10.1007/s13205-025-04323-4","DOIUrl":"10.1007/s13205-025-04323-4","url":null,"abstract":"Fibrolamellar carcinoma (FLC) is a rare form of liver carcinoma with limited diagnostic and therapeutic options. In this study, we utilized the GSE57727 and E-MTAB-1503 datasets, downloaded from GEO and ArrayExpress, respectively, to explore hub genes for FLC diagnosis and potential therapeutic agents. Through the integration of multiple machine learning approaches and drug sensitivity databases, we identified AKTIP as a potential diagnostic biomarker for FLC. AKTIP exhibited markedly elevated expression in FLC compared to non-FLC, demonstrating superior diagnostic and prognostic performance over other FLC-specific biomarkers. Four compounds (PI-103, BVT-948, Digitoxigenin, and SB-218078) were identified as potential therapeutic agents targeting AKTIP. Molecular docking analysis revealed strong binding affinities of these compounds to AKTIP, and molecular dynamics simulations further validated the reliability and rationality of the molecular docking results. Pan-cancer analysis indicated that AKTIP expression varies across different tissues and is significantly associated with patient prognosis. qRT-PCR analysis confirmed that AKTIP mRNA levels were markedly overexpressed in normal liver epithelial cells compared to human hepatocellular carcinoma cell lines. In conclusion, AKTIP was successfully identified as a diagnostic and prognostic biomarker for FLC, and four compounds were proposed as potential therapeutic agents. This study uncovers new perspectives on diagnosing and managing of this rare type of liver carcinoma, offering promising avenues for future research and clinical applications.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04323-4.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 6","pages":"181"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095112/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing the power of stem cell-derived exosomes: a rejuvenating therapeutic for skin and regenerative medicine. 利用干细胞衍生外泌体的力量：皮肤和再生医学的恢复治疗。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-06-01 Epub Date: 2025-05-23 DOI: 10.1007/s13205-025-04345-y

Gracy Seth, Siddharth Singh, Geetansh Sharma, Divyesh Suvedi, Dinesh Kumar, Rupak Nagraik, Avinash Sharma

{"title":"Harnessing the power of stem cell-derived exosomes: a rejuvenating therapeutic for skin and regenerative medicine.","authors":"Gracy Seth, Siddharth Singh, Geetansh Sharma, Divyesh Suvedi, Dinesh Kumar, Rupak Nagraik, Avinash Sharma","doi":"10.1007/s13205-025-04345-y","DOIUrl":"10.1007/s13205-025-04345-y","url":null,"abstract":"Exosomes are small extracellular vesicles produced by most cell types and contain proteins, lipids, and nucleic acids (non-coding RNAs, mRNA, and DNA) that can be released by donor cells to influence the function of recipient cells. Skin photoaging is the premature aging of skin structures caused by prolonged exposure to ultraviolet (UV), as demonstrated by depigmentation, roughness, rhytides, elastosis, and precancerous alterations. Exosomes are associated with aging processes such as oxidative damage, inflammation, and senescence. Exosomes' anti-aging properties have been linked to various in vitro and preclinical investigations. There are still several unanswered questions about the use of MSC exosomes for skin rejuvenation, despite encouraging results. Uncertainty surrounds the precise processes by which exosomes stimulate the creation of collagen, skin tissue via a variety of mechanisms, including reduced matrix metalloproteinase (MMP) expression, increased collagen and elastin production, and modulation of intracellular signaling pathways and intercellular communication. These findings suggest the therapeutic potential of exosomes in skin aging. This review provides information on the molecular mechanisms and consequences of exosome anti-aging.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 6","pages":"184"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring microalgae-based strategies for the management of white spot syndrome virus: a comprehensive review. 探索基于微藻的白斑综合征病毒管理策略：综合综述。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-06-01 Epub Date: 2025-05-19 DOI: 10.1007/s13205-025-04337-y

Amrutha Vellore Mohankumar, Vernita Priya, Sanjana Anbalagan, Vidya Radhakrishnan, Jayakrishna Kandasamy, Tohru Mekata, Sudhakaran Raja

{"title":"Exploring microalgae-based strategies for the management of white spot syndrome virus: a comprehensive review.","authors":"Amrutha Vellore Mohankumar, Vernita Priya, Sanjana Anbalagan, Vidya Radhakrishnan, Jayakrishna Kandasamy, Tohru Mekata, Sudhakaran Raja","doi":"10.1007/s13205-025-04337-y","DOIUrl":"10.1007/s13205-025-04337-y","url":null,"abstract":"Shrimp culture industries faced huge economic losses due to white spot disease (WSD) caused by the white spot syndrome virus (WSSV). Microalgae are unicellular and can be found in both freshwater and marine habitats. In recent days, microalgae such as Chlamydomonas reinhardtii, Dunaliella salina, Synechocystis species, Synechococcus elongatus, Synechococcus species, and Anabaena species have been explored for treating WSSV using various protocols including transgenic algae, recombinant protein, and dsRNA. The development of dsRNA-expressing lines in C. reinhardtii has improved the efficiency of the new vectors p2xTRBL and pSS116, enabling the selection of transgenic cells without needing markers. Producing VP28 dsRNA to fight the white spot syndrome virus (WSSV) in shrimp has proven particularly advantageous. Due to the development of recombinant plasmids that express VP28 genes in microalgae, oral vaccinations against WSSV have proven highly effective and demonstrated great survival rates in shrimp. Moreover, Dunaliella salina transgenic strains have been modified to generate VP28 genes to shield crayfish from WSSV. Because of their antiviral qualities against WSSV, microalgae such as Synechococcus sp. and Anabaena sp. have also been used and may be useful in managing other aquatic viral infections. Through oral vaccination using sustainable and environmentally friendly practices, these microalgae serve as a disease-control strategy in aquaculture, improving the financial standing and income of aquaculture farmers. All microalgae have antiviral properties and act against the white spot syndrome virus (WSSV).","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 6","pages":"178"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12089564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adjunctive use of Streptococcus salivarius M18 probiotic in the treatment of periodontitis: a randomized controlled trial. 辅助使用唾液链球菌M18益生菌治疗牙周炎：一项随机对照试验

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-06-01 Epub Date: 2025-05-28 DOI: 10.1007/s13205-025-04363-w

Wei-Ju Chen, Lavanya Ajay Sharma, Peng Shao, Tia Griffith, Robert Love, Rohit Jain, John Hale, Ajay Sharma

{"title":"Adjunctive use of Streptococcus salivarius M18 probiotic in the treatment of periodontitis: a randomized controlled trial.","authors":"Wei-Ju Chen, Lavanya Ajay Sharma, Peng Shao, Tia Griffith, Robert Love, Rohit Jain, John Hale, Ajay Sharma","doi":"10.1007/s13205-025-04363-w","DOIUrl":"10.1007/s13205-025-04363-w","url":null,"abstract":"This randomized double-blind placebo-controlled clinical trial aimed to evaluate the adjunctive use of Streptococcus salivarius M18 probiotic lozenges in the treatment of periodontitis. Following non-surgical periodontal therapy (NSPT), 55 participants with stage III or IV periodontitis were administered either S. salivarius M18 lozenges (test group) or a placebo for 12 weeks. Clinical assessments, including pocket probing depth (PPD), clinical attachment loss (CAL), bleeding on probing (BoP), and plaque index (PI), were performed at baseline (before treatment), immediately after treatment, and during post-treatment follow-ups at 12 and 24 weeks. Microbial analysis was conducted on the subgingival plaque samples collected. The test group demonstrated significantly improved PPD, BoP, and PI compared to the placebo group at post-treatment follow-ups, although no significant difference was observed in CAL. Microbiological analysis revealed a reduction in periodontal pathogens or a shift in the subgingival microbiota toward a decreased pathogenic profile in the test group. This trial is the first to demonstrate the safety and efficacy of S. salivarius M18 as an adjunctive treatment for periodontitis, supporting its potential for broader clinical use in managing periodontal health.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04363-w.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 6","pages":"192"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119401/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144179873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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