3 Biotech最新文献

{"title":"Construction of engineered <i>Saccharomyces cerevisiae</i> for producing phytosphingosine.","authors":"Siyan Qiu, Jingru Li, Pengtian Xie, Chun Wei, Jie Sun","doi":"10.1007/s13205-025-04417-z","DOIUrl":"https://doi.org/10.1007/s13205-025-04417-z","url":null,"abstract":"<p><p>Phytosphingosine (PHS), a sphingolipid-derived bioactive compound, exhibits multifunctional properties including antimicrobial activity, skin moisturization, and hydration, rendering it highly valuable for cosmetic and pharmaceutical applications. Through systematic metabolic engineering of <i>Saccharomyces cerevisiae</i>, we achieved 82.62 mg/g DCW phytosphingosine (PHS) production via integrated pathway optimization and stress mitigation. Key strategies involved: (1) Knockout of <i>LCB4</i> (sphingoid long-chain base kinase), <i>SHM2</i> (serine hydroxymethyltransferase), and <i>CHA1</i> (l-serine deaminase) to block competitive pathways; (2) Overexpression of <i>TSC10</i> (3-ketosphingosine reductase), <i>SYR2</i> (sphingosine hydroxylase), and <i>LCB1/LCB2</i> (serine palmitoyltransferase) to amplify the PHS synthesis flux. Initial shake flask fermentation (96 h) yielded 15.31 mg/g DCW of PHS, with <i>ORM2</i> knockout providing a 73.6% productivity increase (26.54 mg/g DCW) despite inducing growth defects from sphingosine accumulation. We hypothesized that disrupted ORM2-mediated control of serine palmitoyltransferase activity might compromise ER homeostasis through sphingolipid imbalance, which was alleviated through <i>HAC1</i> overexpression to enhance unfolded protein response (UPR) capacity. Fed-batch fermentation under optimized conditions (40 mM serine, 0.5 mM palmitic acid, pH 5) demonstrated scalable production, delivering a 5.4-fold improvement over baseline. This work establishes UPR engineering as a critical strategy for resolving lipid toxicity constraints in yeast sphingolipid biosynthesis, while highlighting <i>S. cerevisiae</i>'s potential as an industrial PHS production platform through coordinated pathway and stress response manipulation.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04417-z.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"247"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the prognostic signature of nonsmall cell lung cancer using cisplatin resistance and circulating tumor cell-related gene analysis.","authors":"Linlu Gao, Xiaoyuan Sun, Lei Wang, Kun Gao, Lianyang Yu, Yanying Wang","doi":"10.1007/s13205-025-04402-6","DOIUrl":"https://doi.org/10.1007/s13205-025-04402-6","url":null,"abstract":"<p><p>This study aimed to develop a prognostic prediction model for nonsmall cell lung cancer (NSCLC) based on cisplatin resistance and circulating tumor cell (CTC)-related genes and to explore the molecular mechanisms of key genes. After downloading and preprocessing data from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases, a prognostic signature was developed using least absolute shrinkage and selection operator (LASSO) regression analysis. Patients were categorized into low-risk (LR) and high-risk (HR) groups based on their risk score (RS), and differences in survival, immune infiltration, and immunotherapy/chemotherapy responses were compared. A nomogram was established for clinical application, followed by identification of molecular subtypes. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was used to verify the expression of key genes in the A549 and A549/DDP cell lines. Finally, we explored the molecular mechanisms by which inhibition of FAM83A reversed cisplatin resistance. Using LASSO regression, a prognostic signature with eight genes was developed; the survival rate of patients in the HR group was significantly lower than that of patients in the LR group (all <i>P</i> < 0.05). The RS was an independent risk factor for NSCLC and stably predicted the prognosis (<i>P</i> < 0.05). The immune infiltration levels of LR and HR patients were significantly different, and the model could effectively predict the response to drugs or immunotherapy (all <i>P</i> < 0.05). Subsequently, subtype clustering divided the patients into two groups to assist in identifying heterogeneity between patients with different risks. Finally, qRT-PCR confirmed that these genes were aberrantly expressed in A549 cells and were closely associated with cisplatin resistance (all <i>P</i> < 0.05). Notably, FAM83A overexpression promoted the proliferation and invasion and inhibited the apoptosis of A549 cells (all <i>P</i> < 0.05). Low FAM83A expression reversed cisplatin resistance in lung cancer cells (<i>P</i> < 0.05). The eight-gene model constructed in this study can predict the prognosis of patients with NSCLC and guide personalized treatment. In addition, targeted inhibition of FAM83A expression can reverse cisplatin resistance, potentially enhancing the efficacy of chemotherapy in combination with immunotherapy, thereby providing a novel strategy for clinical practice.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"259"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12271037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into the eminent biotechnological applications of xylanolytic enzymes for sustainable bioprocessing.","authors":"Fatima Akram, Momena Safdar, Ifrah Shabbir, Taseer Fatima, Ikram-Ul-Haq","doi":"10.1007/s13205-025-04415-1","DOIUrl":"https://doi.org/10.1007/s13205-025-04415-1","url":null,"abstract":"<p><p>Xylan is one of the most abundant polysaccharides in nature and presents a structural complexity characterized by a heterogeneous polymer composition. Comprising various sugar subunits and associated acids linked through a diverse array of bonds, xylan poses challenges for complete degradation. This review article provides a comprehensive overview of xylan's structure, the role of xylanolytic enzymes in its degradation, and the industrial applications of xylanases in sectors, such as paper and pulp, food, textiles, and pharmaceuticals. Furthermore, it also discusses the use of advanced biotechnology tools, such as nano-biotechnology and genetic engineering, particularly through CRISPR/CAS technology, for enhancing the thermostability of xylanases. This article also provides insights into emerging trends in xylanase research, including bioprospecting novel thermostable xylanases from metagenomes, protein engineering, synthetic biology, and the integration of biorefinery. Finally, it highlights the importance of regulatory frameworks and standardization initiatives for ensuring the quality and the sustainability of xylanase-based technologies. Overall, this review offers valuable insights into the multifaceted role of xylanases in biotechnology and industrial bioprocessing while outlining future directions for research and innovation in this field.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"249"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12245751/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dual threat: exploring the emergence of human metapneumovirus and SARS-CoV-2 coinfections in respiratory infections.","authors":"Falguni Goel, Daksh Kumar, Payal Singh, Sachchida Nand Rai","doi":"10.1007/s13205-025-04384-5","DOIUrl":"https://doi.org/10.1007/s13205-025-04384-5","url":null,"abstract":"<p><p>SARS-CoV-2-triggered COVID-19 epidemic has thrown unprecedented challenges at the global public health system but has also drawn attention to the co-circulation of other respiratory viruses, among them human metapneumovirus (hMPV). Both viruses are significant contributors to respiratory illnesses. Human metapneumovirus (hMPV) can cause serious respiratory infections, particularly in young children, elderly, and immunocompromised individuals. This review explores the overlap between human metapneumovirus (hMPV) and SARS-CoV-2, focusing on their epidemiological patterns, clinical manifestations, and the impact of co-infection on disease severity and patient outcomes. We consider the molecular mechanisms of hMPV's interaction with the host immune system, which might have an impact or exacerbate SARS-CoV-2 pathogenicity. The challenges of identifying and treating co-infections are also discussed in this study as are the effects of clinical management during the present pandemic. Furthermore, the possibilities of synergistic effects between the two viruses involved in these coinfections, particularly modified immune response and worsened respiratory outcomes, have been brought up. We address the intersection of these two viral pathogens to provide insights into their combined burden on healthcare systems, underscore the need for improved diagnostic tools, and advocate for research on targeted treatment and vaccine strategies. This review highlights the dual threat posed by hMPV and SARS-CoV-2 and calls for greater attention to the implications of viral co-infections in respiratory diseases.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"235"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12229485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of potential target sites in cotton leaf Curl Multan Virus (CLCuMuV) after hybridization through candidate miRNAs in cotton to induce virus resistance.","authors":"Momna Dar, Tauheed Suddal, Mudassar Fareed Awan, Muhammad Farhan Sarwar, Sana Shakoor, Safia Obaidur Rab","doi":"10.1007/s13205-025-04401-7","DOIUrl":"https://doi.org/10.1007/s13205-025-04401-7","url":null,"abstract":"<p><p>The present study focuses on the computational identification and experimental validation of <i>Gossypium hirsutum</i>-derived miRNAs capable of targeting the <i>CLCuMuV</i> genome. The retrieved <i>CLCuMuV</i> genome sequence and 80 mature miRNAs were subjected to the identification of six key open reading frames (ORFs) encoding viral proteins: AC4 (100 amino acids), AC3 (134 amino acids), AC2 (150 amino acids), Rep (363 amino acids), AV2 (121 amino acids), and coat protein (256 amino acids). Using a combination of computational tools, including psRNATarget, RNA22, RNAhybrid, and miRanda, potential miRNA-mRNA interactions were predicted based on sequence complementarity and free energy calculations. The computational analysis revealed multiple cotton miRNAs manifesting increased binding potential to viral mRNAs. The potential miRNAs included ghr-miR390a, ghr-miR7486a, and ghr-miR7503 which emerged as highly promising candidates for RNA interference. Among these, ghr-miR390a witnessed the highest level of binding efficiency equipped with duplex free energy (ΔG Duplex) of - 18.70 kcal/mol with binding energy (ΔG Binding) of - 16.78 kcal/mol, indicating the maximum potential of inhibiting viral gene expression. These cotton-derived potential miRNAs target essential viral genes involved in replication, coat protein synthesis, and movement immensely critical for the survival and proliferation of <i>CLCuMuV</i>. Phylogenetic analysis revealed that <i>CLCuMuV</i> isolates share common evolutionary ancestors, suggesting potential cross-resistance mechanisms that could be exploited. To experimentally validate these computational predictions, the identified miRNAs were cloned and highly expressed in transgenic cotton lines. Real-time PCR (RT-PCR) was employed to confirm the expression level of the target miRNAs in the transgenic plants while enzyme-linked immunosorbent assay (ELISA) validated the presence of key <i>GhDCL3</i> proteins in transgenic in contrast to the control. The transgenic lines expressing the ghr-miR390a encoding gene <i>GhDCL3</i> witnessed a significant reduction in symptoms of <i>CLCuMuV</i>, and incommensurable presence of <i>CLCuMuV</i> infection, while control plants exhibited typical disease symptoms, including leaf curling and vein thickening. This study identifies three highly promising miRNAs ghr-miR7486a, ghr-miR390a, and ghr-miR7503 that can effectively target critical genes in the <i>CLCuMuV</i> genome, providing a molecular mechanism to inhibit viral replication and mitigate the impact of the disease. The successful integration of these miRNAs into cotton via genetic engineering offers a novel and sustainable approach to control <i>CLCuMuV</i>.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"234"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12222586/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the viral footprint: comprehensive genomic exploration of endogenous viral elements in <i>Musa</i> spp. and <i>Ensete</i> sp.","authors":"Anuradha Chelliah, Chandrasekar Arumugam, Selvarajan Ramasamy","doi":"10.1007/s13205-025-04412-4","DOIUrl":"https://doi.org/10.1007/s13205-025-04412-4","url":null,"abstract":"<p><p>Endogenous viral elements (EVEs) are virus-derived sequences integrated into host germline genomes, propagating across generations and potentially becoming fixed through natural selection or genetic drift. While extensively studied in animals, EVEs have been less frequently reported in plants. This study performed a comprehensive genome-wide analysis across 15 species, subspecies, and cultivars of <i>Musa</i> and <i>Ensete</i> to identify sequences associated with viral interactions. Our analysis revealed EVEs associated with four plant virus families: <i>Tospoviridae</i>, <i>Caulimoviridae</i>, <i>Solemoviridae</i>, and <i>Mimiviridae</i>. Phylogenetic analysis showed that these EVEs clustered with their corresponding viruses, with viruses from the same family grouping together. We identified 11 distinct Banana Streak Virus species across <i>Musa</i> genomes. Most corresponding EVEs were annotated as hypothetical proteins, while others aligned with functional proteins, including Polyprotein P3, movement protein, capsid protein, protease, reverse transcriptase, and ribonuclease H. These EVEs appear to be stable components of the <i>Musa</i> genome, which may potentially contribute to growth, development, metabolism, and stress responses. This study provides insights into virus-host interactions and evolutionary relationships among <i>Musa</i> and <i>Ensete</i> species, highlighting the prevalence and distribution of EVEs in <i>Musa</i> genomes and offering a foundation for future investigations into their functional roles and evolutionary significance.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04412-4.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"242"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12227380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptations, diversity, and biotechnological potential of thermophilic microorganisms: exploring culture-dependent and culture-independent approaches in extreme environments.","authors":"Sonal Gupta, Vatsal Bhargava, Jasvinder Kaur, Pushp Lata","doi":"10.1007/s13205-025-04382-7","DOIUrl":"https://doi.org/10.1007/s13205-025-04382-7","url":null,"abstract":"<p><p>Thermophilic prokaryotes exhibit remarkable adaptations at both physiological and molecular levels, which enables them to survive in elevated-temperature environments (35-113 °C). This review consolidates the current findings on membrane, genomic and proteomic adaptations of thermophiles, including presence of ether-linked lipids, branched-chain fatty, saturated long-chain hydrocarbons, reverse gyrase-mediated DNA supercoiling, and group II chaperonins. Although culture-independent approaches, such as metagenomics and next-generation sequencing (NGS) techniques, have expanded our understanding of thermophilic microbial diversity, which is limited by traditional culture-dependent approaches. However, these findings are largely based on the genomic predictions rather than direct experimental evidence. Therefore, the culture-dependent methods are crucial for isolating and characterizing thermophiles for both genetic and functional studies. India's geothermal spring ecosystems serve as natural laboratories for studying diverse thermophilic communities, offering a valuable resource for both ecological and biotechnological exploration. Enzymes derived from thermophiles, known as thermophiles exhibit remarkable thermal stability and catalytic efficiency at temperatures ranging from 60 to 125 °C, with applications in biofuels, pharmaceuticals, and industrial bioprocessing. Integration of both culture-based and metagenomic approach provide a comprehensive framework for understanding the thermophile biology and reveals their biotechnological potential.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"232"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12222611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144574677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic gold nanoparticles synthesized using <i>Streptomyces</i> sp. M137-2 as potential vaccine adjuvant.","authors":"Nefise Ünlüer, Aytül Gül-Mete, Elif Esin Hameş","doi":"10.1007/s13205-025-04389-0","DOIUrl":"https://doi.org/10.1007/s13205-025-04389-0","url":null,"abstract":"<p><p>In this study, we describe the adjuvanticity of biogenic AuNPs, previously synthesized as 40-50 nm spherical particles from cell-free fermentation broth of <i>Streptomyces</i> sp. M137-2. Bovine serum albumin (BSA), a model antigen, was conjugated to biogenic AuNPs to form AuNPs-BSA. The conjugates were characterize using UV-Vis spectroscopy, scanning electron microscopy (SEM), and Fourier-transform infrared spectroscopy (FT-IR). Both biogenic AuNPs and AuNPs-BSA showed high viability (over 70%) in macrophages derived from U937 cells. Biogenic AuNPs enhanced the uptake of conjugated BSA by macrophages. AuNPs-BSA-stimulated macrophages exhibited cytokine levels of TNF-α and IL-6 that were 4.5 times (<i>p</i> < 0.0001) and 4.6 times (<i>p</i> = 0.004) higher, respectively, as compared to BSA-stimulated cells. The adjuvanticity of the biogenic AuNPs is likely attributed to their antigen-carrier properties, which enhance antigen uptake by macrophages, as well as their immunostimulatory properties that induce strong pro-inflammatory responses. This study represents the first proposal of biogenic AuNPs as an adjuvant candidate, providing further studies in in vivo models.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"244"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12234941/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GWAS and haplotype analysis reveal the intricate interplay of hormones in rice seed germination under hypoxic conditions.","authors":"C Vijay Kumar Reddy, Siddharth Panda, Anthony J Travis, Gareth J Norton, Sanjay Kumar, Anandan Annamalai, Adam H Price","doi":"10.1007/s13205-025-04422-2","DOIUrl":"https://doi.org/10.1007/s13205-025-04422-2","url":null,"abstract":"<p><p>Genome-wide association mapping of the BAAP panel revealed 14 QTLs significantly associated with anaerobic germination tolerance (AGT), distributed across eight chromosomes. Among these, chromosome 11 emerged as a key region, harbouring two prominent QTL hotspots qAG11.1 and qAG11.2 indicating its major contribution to AGT in direct-seeded rice under flooded conditions. Candidate gene analysis revealed strong associations with key regulators of hormonal and metabolic pathways. Notably, ethylene biosynthesis and signalling genes-OsACO (LOC_Os09g39720, LOC_Os06g37590), CTR1 (LOC_Os09g39320), and ERS1 (LOC_Os03g49500) were linked to enhanced coleoptile elongation under hypoxia. EMF2B (LOC_Os09g13630), involved in ABA-mediated dormancy regulation, and KO1 (LOC_Os06g37330), a critical enzyme in GA biosynthesis, contributed to early seedling vigour. SLR1 (LOC_Os03g49990), a GA signalling repressor, was functionally linked to growth regulation under anaerobic stress. Carbohydrate mobilization genes β-amylase (LOC_Os09g39570) and TPP6 (LOC_Os08g31630) were also strongly associated with AGT performance. Haplotype analysis identified favourable alleles across multiple loci, with genotypes such as ARC 14940 and Boro Black combining superior haplotypes and exhibiting higher germination rates and shoot elongation. These findings provide a robust genetic framework and valuable markers for accelerating AGT breeding in rice through haplotype-based selection.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04422-2.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"250"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12254463/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144625254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-bacterial activity of silibinin-functionalized silica-coated Fe₃O₄ magnetic nanocomposites on <i>Pseudomonas aeruginosa</i>.","authors":"Nazanin Pasandideh Kordmahaleh, Mirsasan Mipour, Najmeh Ranji, Mahdi Shahriarinour, Mohammad Nikpassand","doi":"10.1007/s13205-025-04371-w","DOIUrl":"https://doi.org/10.1007/s13205-025-04371-w","url":null,"abstract":"<p><p>The nanocomposites were synthesized and characterized using Fourier transform infrared spectroscopy (FT-IR), thermogravimetric analysis (TGA), transmission electron microscopy (TEM), field emission scanning electron microscopy (FE-SEM), and X-ray diffraction (XRD). The physicochemical analyses confirmed the structural integrity, uniform particle size (34-58 nm), thermal stability (> 600 °C), and magnetic properties of the nanocomposites. The nanocomposites exhibited strong synergistic effects with ciprofloxacin, reducing the minimum inhibitory concentration (MIC) in combination by 4- to 16-fold compared to ciprofloxacin alone. Furthermore, the nanocomposites in combination with ciprofloxacin significantly inhibited biofilm formation in clinical isolates. The gene expression analysis revealed downregulation of key efflux pump genes (<i>mexX, mexY,</i> and <i>oprM</i>), resulting to increased intracellular accumulation of ciprofloxacin. This mechanism potentiated the bactericidal effects of ciprofloxacin, even against resistant strains. These findings highlight the potential of Fe₃O₄@SPN@Silibinin as a biodegradable, stable, and water-soluble nanocarrier for silibinin delivery, offering a novel therapeutic strategy for combat multidrug-resistant bacterial infections. This study presents a promising approach to addressing the growing threat of antibiotic resistance through targeted drug delivery and genetic modulation.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"253"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12255613/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}