3 Biotech最新文献

{"title":"Gene characterization of <i>Chenopodium quinoa</i> Willd. and <i>Chenopodium album</i> L. accessions: unmasking genetic diversity.","authors":"Babita Kumari, Nikhil Kumar Chrungoo","doi":"10.1007/s13205-024-04173-6","DOIUrl":"10.1007/s13205-024-04173-6","url":null,"abstract":"<p><p>The genus <i>Chenopodium</i> L. includes the domesticated seed crop <i>Chenopodium quinoa</i> Willd. and the semi-domesticated seed/fodder <i>Chenopodiumalbum</i> L., both valued for their high protein content and high-quality grains. This study investigates the morphological and molecular characteristics of starch granules in 50 accessions including <i>C. quinoa</i> Willd. and <i>C. album</i> L<i>.</i>, to elucidate variations in amylose content and genetic markers. Starch granules were analyzed using scanning electron microscopy, revealing primarily angular to polygonal shapes with an average size of ~ 1.5 µm. Dynamic light scattering showed size variation: <i>C. album</i> L. granules ranged from 115.1 ± 8.09 to 192.5 ± 5.11 nm, while <i>C. quinoa</i> Willd. granules from 204.5 ± 21.45 to 263.9 ± 12.48 nm. Apparent amylose content (AAC) was categorized via iodine staining into high (> 25%), intermediate (19-25%), low (11-19%), and very low (5-12%) classes. The results demonstrated a wide AAC range, with <i>C. album</i> L. displaying a broader spectrum compared to <i>C. quinoa</i> Willd. The molecular characterization of the <i>Waxy</i> locus, crucial for amylose synthesis, was performed using PCR and sequencing. The <i>Waxy</i> locus, consisting of 13 exons and 12 introns, showed significant sequence similarity with <i>Chenopodium</i> species. The key single nucleotide polymorphisms (SNPs) associated with AAC levels were identified, including variations in exons 1, 4, 6, 9, and 13. A 100 bp deletion in intron 9 was specific to <i>C. album</i> L<i>.</i>, facilitating the development of an allele-specific PCR marker to distinguish between <i>C. quinoa</i> Willd. and <i>C. album</i> L. The phylogenetic analysis of <i>Waxy</i> sequences divided accessions into two primary clusters, reflecting their A-genome and B-genome origins. The study enhances understanding of genetic diversity and offers insights for breeding applications in <i>Chenopodium</i> species.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04173-6.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"9"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11646240/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142833406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro Genebank of India for safe conservation of horticultural plant diversity: four decades of milestones.","authors":"Ravi Gowthami, Anuradha Agrawal, Subhash Chander, Ruchira Pandey, Neelam Sharma, Sandhya Gupta, Vartika Srivastava, Era Vaidya Malhotra, Sangita Bansal, Surendra Kumar Malik, Anju Mahendru-Singh, Gyanendra Pratap Singh","doi":"10.1007/s13205-024-04177-2","DOIUrl":"10.1007/s13205-024-04177-2","url":null,"abstract":"<p><p>India is a treasure trove of biological diversity with its plant genetic resources playing a crucial role in the crop improvement serving as the foundation for the country's sustainable food and nutritional security. India's in vitro genebank (IVG) is part of the National Genebank at the Indian Council of Agricultural Research-National Bureau of Plant Genetic Resources (ICAR-NBPGR). The IVG houses distinctive multi-crop repository that utilizes several tissue culture techniques for short- to medium-term storage in in vitro active genebank (IVAG) and cryoconservation approaches for long-term storage in in vitro base genebank (IVBG). In IVAG, germplasm is conserved under normal and slow growth conditions with a subculture period of 1-36 months depending on the species/genotype and conservation approach. Currently, the IVAG holds 2,038 germplasm accessions (69 genera and 171 species) from six crop groups, viz<i>.</i> (a) tropical fruit crops (449), (b) temperate and minor tropical fruit crops (408), (c) tuber crops (530), (d) bulbous and ornamental crops (187), (e) medicinal and aromatic plants (232), and (f) spices and industrial crops (232). For long-term conservation, in vitro produced explants of various species are cryopreserved at the IVBG in liquid nitrogen. Utilizing various cryoconservation procedures, 347 accessions from several crop groups have been successfully conserved in the IVBG. Over the past four decades, in vitro conservation has been accomplished by the above mentioned cutting-edge techniques. This report highlights the efforts and achievements of the National Genebank in conserving horticultural genetic resources through in vitro and cryoconservation.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"11"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11649606/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut-brain axis and brain health: modulating neuroinflammation, cognitive decline, and neurodegeneration.","authors":"Anchal Trisal, Ishika Singh, Geetika Garg, Khanak Jorwal, Abhishek Kumar Singh","doi":"10.1007/s13205-024-04187-0","DOIUrl":"10.1007/s13205-024-04187-0","url":null,"abstract":"<p><p>The microbiota-gut-brain axis is a pivotal medium of crosstalk between the central nervous system (CNS) and the gastrointestinal tract. It is an intricate network of synergistic molecular pathways that exert their effects far beyond their local vicinity and even affect the systemic functioning of the body. The current review explores the involvement of the gut-brain axis (GBA) in the functioning of the nervous system, with a special emphasis on the neurodegeneration, cognitive decline, and neuroinflammation that occur in Alzheimer's disease (AD) and Parkinson's disease (PD). Gut-derived microbial metabolites play an important role in facilitating this interaction. We also highlighted the complex interaction between gut-derived metabolites and CNS processes, demonstrating how microbial dysbiosis might result in clinical disorders. Short-chain fatty acids have neuroprotective properties, whereas branched-chain amino acids, trimethylamine-N-oxide (TMAO), and tryptophan derivatives such as indole have negative effects at high concentrations. Furthermore, we cover pharmaceutical and nonpharmacological approaches for restoring the gut microbial balance and promoting neurological health. We further expanded on nutritional therapies and lifestyle changes, such as the Mediterranean diet and exercise. Next, we focused on food-controlling habits such as caloric restriction and intermittent fasting. Moreover, interventional techniques such as prebiotics, probiotics, and pharmacological medications have also been utilized to modify the GBA. Historical microbiome research from early discoveries to recent studies linking gut health to cognitive and emotional well-being has increased our understanding of the GBA.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"25"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680542/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic potential inhibitor for dipeptidyl peptidase IV in diabetic type 2: in silico approaches.","authors":"Miah Roney, Amit Dubey, Md Nazim Uddin, Abdul Rashid Issahaku, Aisha Tufail, Nasir Tufail, Anke Wilhelm, Mohd Fadhlizil Fasihi Mohd Aluwi","doi":"10.1007/s13205-024-04200-6","DOIUrl":"10.1007/s13205-024-04200-6","url":null,"abstract":"<p><p>Diabetes mellitus (DM) is a metabolic disease marked by an excessive rise in blood sugar (glucose) levels caused by a partial or total absence of insulin production, combined with alterations in the metabolism of proteins, lipids, and carbohydrates. The International Diabetes Federation estimates that 425 million individuals globally had diabetes in 2017 which will be 629 million by 2045. Several medications are used to treat DM, but they have limitations and side effects including weight gain, nausea, vomiting, and damage to blood vessels and kidneys. Therefore, it is essential to identify anti-diabetic drugs that have less or no side effects. Hence, the current study employed in silico approaches to discover new DPP-IV inhibitors that might be associated with diabetes. Thirty-four (34) co-crystalized DPP-IV enzymes were found from the protein data bank and the co-crystal ligands were docked into the active-site 6B1E protein to find out the hit compounds. From the docking results, we found two hit compounds (5T4E and 4J3J) which were used to find out the analogs from the experimental drug database using the DrugRep software. According to the results, twenty (20) analogs were found from the experimental drug database with the similarity score of ≥ 0.790 and docked once again into the active site of the DPP-IV (PDB ID: 6B1E) enzyme. Interestingly, DB02226 showed the best binding affinity (-10.3 kcal/mol) and prime MM/GBSA (-68.73 kcal/mol) compared to the reference drug (co-crystal ligand; -7.4 kcal/mol and -47.49 kcal/mol, respectively). Additionally, DB02226 has shown excellent reactivity, efficacy, and structural stability in the binding region of target proteins in studies using MD simulation, MM/GBSA, DFT, and MESP analysis. These findings can be utilized to support further in vitro, in vivo, pre-clinical and clinical research rather than definitively confirming anti-diabetic effectiveness.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"24"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680545/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the potential of <i>Acinetobacter calcoaceticus</i> in alleviation of aluminium stress in <i>Triticum aestivum</i>.","authors":"Mohammad K Okla, Sadia Javed, Muhammad Faran Tahir, Muhammad Anas, Muhammad Hamzah Saleem, Temoor Ahmed, Ibrahim A Saleh, Naser Zomot, Yasmeen A Alwasel, Mostafa A Abdel-Maksoud, Shafaqat Ali, Shah Fahad","doi":"10.1007/s13205-024-04192-3","DOIUrl":"10.1007/s13205-024-04192-3","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Soil contamination with toxic heavy metals [such as aluminum (Al)] is becoming a serious global problem due to the rapid development of the social economy. Although plant growth-promoting rhizo-bacteria (PGPR) are the major protectants to alleviate metal toxicity, the study of these bacteria to ameliorate the toxic effects of Al is limited. Therefore, the present study was conducted to investigate the combined effects of different levels of &lt;i&gt;Acinetobacter calcoaceticus&lt;/i&gt; (5 ppm and 10 ppm) of accession number of MT123456 on plant growth and biomass, photosynthetic pigments, gas exchange attributes, oxidative stress and response of antioxidant compounds (enzymatic and nonenzymatic), and their specific gene expression, sugars, nutritional status of the plant, organic acid exudation pattern and Al accumulation from the different parts of the plants, which was spiked with different levels of Al [0 µM (i.e., no Al), 50 µM, and 100 µM] using aluminum sulfate [Al&lt;sub&gt;2&lt;/sub&gt;(SO&lt;sub&gt;4&lt;/sub&gt;)&lt;sub&gt;3&lt;/sub&gt;] in wheat (&lt;i&gt;Triticum aestivum&lt;/i&gt; L.). Results from the present study revealed that the Al toxicity induced a substantial decreased in shoot length, root length, number of leaves, leaf area, shoot fresh weight, root fresh weight, shoot dry weight, root dry weight, chlorophyll-a, chlorophyll-b, total chlorophyll, carotenoid content, net photosynthesis, stomatal conductance, transpiration rate, soluble sugar, reducing sugar, non-reducing sugar contents, calcium (Ca&lt;sup&gt;2+&lt;/sup&gt;), magnesium (Mg&lt;sup&gt;2+&lt;/sup&gt;), iron (Fe&lt;sup&gt;2+&lt;/sup&gt;), and phosphorus (P) contents in the roots and shoots of the plants. In contrast, increasing levels of Al in the soil signifcantly (&lt;i&gt;P&lt;/i&gt; &lt; 0.05) increased Al concentration in the roots and shoots of the plants, phenolic content, malondialdehyde (MDA), hydrogen peroxide (H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt;), electrolyte leakage (EL), fumaric acid, acetic acid, citric acid, formic acid, malic acid, oxalic acid contents in the roots of the plants. Although, the activities of enzymatic antioxidants such as superoxidase dismutase, peroxidase, catalase, ascorbate peroxidase and their specific gene expression in the roots and shoots of the plants and non-enzymatic such as phenolic, favonoid, ascorbic acid, and anthocyanin contents were initially increased with the exposure of 50 µM Al, but decreased by the increasing the Al concentration 100 µM in the soil. Addition of &lt;i&gt;A&lt;/i&gt;. &lt;i&gt;calcoaceticus&lt;/i&gt; into the soil signifcantly alleviated Al toxicity effects on &lt;i&gt;T&lt;/i&gt;. &lt;i&gt;aestivum&lt;/i&gt; by improving photosynthetic capacity and ultimately plant growth. Increased activities of antioxidant enzymes in &lt;i&gt;A&lt;/i&gt;. &lt;i&gt;calcoaceticus&lt;/i&gt;-treated plants seem to play a role in capturing stress-induced reactive oxygen species as was evident from lower levels of MDA, H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt;, MDA, and EL in &lt;i&gt;A&lt;/i&gt;. &lt;i&gt;calcoaceticus&lt;/i&gt;-treated plants. Research findings, therefore, suggested that &lt;i&gt;A&lt;/i&gt;. &lt;i&gt;calcoaceticus&lt;/i&gt; application can a","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"34"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11704110/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142942417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An <i>in - silico</i> perspective on the role of methylation-related genes in wheat - <i>Fusarium graminearum</i> interaction.","authors":"Lalit L Kharbikar, Arti S Shanware, Shweta K Nandanwar, Mahender S Saharan, Sarmistha Nayak, Sushma Rani Martha, Ashish Marathe, Anil Dixit, Neeti Sanan Mishra, Simon G Edwards","doi":"10.1007/s13205-024-04179-0","DOIUrl":"10.1007/s13205-024-04179-0","url":null,"abstract":"<p><p>Wheat (<i>Triticum aestivum</i> L.), a vital cereal crop, provides over 20% of the total calories and protein in the human diet. However, <i>Fusarium graminearum</i>, the pathogen responsible for Fusarium head blight (FHB), poses a significant threat to wheat production by contaminating grains with harmful mycotoxins. Although Fusarium head blight is currently a minor disease in India, it has the potential to cause substantial yield and quality losses, especially if rain occurs during mid-anthesis. Epigenetic mechanisms, including DNA methylation and sRNA accumulation, are crucial in regulating gene expression and enabling plants to adapt to environmental stresses. Previous studies investigating wheat's response to <i>F</i>. <i>graminearum</i> through transcriptome analysis of lines differing in 2DL FHB resistance QTLs did not fully explore the role of methylation-related genes. To address this gap, we re-analyzed RNA-Seq data to uncover the response of methylation-related genes to pathogen infection. Our analysis revealed that 16 methylation-related genes were down-regulated in the susceptible line 2-2890, with Gene Ontology (GO) analysis linking these genes to L-methionine salvage from methylthioadenosine (GO:0019509), S-adenosylmethionine metabolism (GO:0033353), and steroid biosynthesis (GO:0006694) (<i>p</i>-value = 0.001). Co-expression analysis identified a negative correlation (-0.82) between methionine S-methyl-transferase (MSM; TraesCS1A02G013800) and 3-hydroxy-3-methyl-glutaryl coenzyme A reductase (HMGCR; TraesCS5A02G269300). HMGCR also showed negative correlations (-1.00) with genes encoding pathogenesis-related, detoxification proteins, and xylanase inhibitors, with GO associating these genes with methionine S-methyl transferase activity (<i>p</i>-value = 0.001). In pathogen-inoculated samples, the elevated expression of HMGCR (Log2 3.25-4.00) and the suppression of MSM (Log2 1.25-3.25) suggest a dual role in stress response and susceptibility, potentially linked to disrupted DNA methylation and isoprenoid biosynthesis pathways. Furthermore, 43 genes down-regulated by miR9678 were associated with biotic stimulus responses and glucan endo-1,4-beta-glucanase activity, highlighting the complex regulatory networks involved in wheat's defense against <i>F</i>. <i>graminearum</i>. This study reveals the roles of methylation-related genes in susceptible wheat lines 2-2890, providing new insights into their potential impact on pathogen response and plant susceptibility.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04179-0.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"12"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11649892/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cleome arabica</i> L mitigates bisphenol A-induced ovarian dysfunction and inflammation in Wistar rats: biochemical, histopathological, pharmacokinetic, and in silico studies.","authors":"Ikram Allagui, Jazia Sdayria, Khaled Athmouni, Nourhene Zammel, Fatma Guesmi, Mongi Saoudi, Angelo Maria Giuffrè, Mohamed Salah Allagui, Saber Nahdi, Abdel Halim Harrath","doi":"10.1007/s13205-024-04169-2","DOIUrl":"10.1007/s13205-024-04169-2","url":null,"abstract":"<p><p>The present study evaluated the antioxidant and anti-inflammatory properties of <i>Cleome arabica</i> (CA) fruit extract against bisphenol A (BPA)-induced ovarian injury in female Wistar rats. The antioxidant activity was estimated by the total antioxidant capacity (TAC) and superoxide radical (NBT) content. For the in vivo analyses, 24 animals were divided into the following 4 groups: the control group; the BPA group (50 mg/kg BW BPA for 30 days); the BPA  + CA group (50 mg/kg BW BPA and 50 mg/kg BW CA); and the CA group (50 mg/kg BW CA). The in vitro results demonstrated that CA exhibited strong antioxidant activity and scavenged O₂•- radicals. . Pharmacokinetic properties were also explored, reflecting the physiological dynamics of the five active molecules (quercetin, catechin, kaempferol, rosmarinic acid, and naringenin). The in vivo findings revealed a significant increase in body weight associated with a significant increase in plasma C-reactive protein (CRP), proinflammatory cytokines (IL-1, IL-6, and TNF-α), and testosterone levels (<i>p</i> < 0.01). In addition, ovarian histological disruption was observed. However, co-administration of CA extract significantly improved ovarian histological integrity and attenuated inflammatory and androgenic disturbances. Moreover, in silico investigations showed that CA compounds interacted more strongly with the active sites of IL-1β, IL-6, or TNF-α. The best binding energy was observed between catechin (five H-bonds) and IL-1β and IL-6, at -6.0 and -6.1 kcal/mol, respectively, and between rosmarinic acid (four H-bonds) and TNF-α, at -6.4 kcal/mol. The present study supports the use of <i>Cleome arabica</i> in the treatment of infertility for female polycystic ovary syndrome (PCOS) patients.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"21"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11663833/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142885210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the therapeutic effects of Shenzhiling oral liquid on Alzheimer's disease: a network pharmacology and experimental approach.","authors":"Gaofeng Qin, Rongqiang Song, Jingyi Sun, Bing Chen, Zhe Liu, Lei Han, Baoliang Sun, Chen Li","doi":"10.1007/s13205-024-04181-6","DOIUrl":"10.1007/s13205-024-04181-6","url":null,"abstract":"<p><p>There is currently no effective treatment for Alzheimer's disease (AD). This research explored Shenzhiling Oral Liquid (SZLD) against AD by pinpointing crucial elements and understanding its molecular mechanisms through network pharmacology and in vitro experiment. First, we used network pharmacology to screen the main targets and mechanisms of SZLD to improve AD. Then we conducted experiments with Aβ42-induced SH-SY5Y cells to assess SZLD's impact, focusing particularly on apoptotic pathways, thereby uncovering its mechanism of action in AD. Through our analysis, we discovered a notable link between SZLD's effect on AD and apoptosis processes. Specifically, the critical proteins Casapse3 and BCL-2 showed strong correlations in this context. Through systematic data analysis and experimental verification, we unveiled the healing advantages and the foundational molecular mechanisms of SZLD in AD. These findings underscore the promising and compelling potential of targeting the PI3K/Akt signaling pathway and apoptosis with SZLD as a therapeutic strategy to ameliorate AD.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"14"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11652558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of novel inhibitors from <i>Rubus ellipticus</i> as anti-leishmanial agents targeting DDX3-DEAD box RNA helicase of <i>Leishmania donovani</i>.","authors":"Vinita Gouri, Gargi Roy, Akanksha Kanojia, Sumeet Singh, Rohini Muthuswami, Mukesh Samant","doi":"10.1007/s13205-024-04183-4","DOIUrl":"10.1007/s13205-024-04183-4","url":null,"abstract":"<p><p>Visceral leishmaniasis (VL), caused by <i>Leishmania donovani</i>, remains challenging to treat due to severe side effects and increasing drug resistance associated with current chemotherapies. Our study investigates the anti-leishmanial potential of <i>Rubus ellipticus</i> from Uttarakhand, India, with extracts prepared from leaves and stems using ethanol and hexane. Advanced GC-MS analysis identified over 100 bioactive compounds, which were screened using molecular docking to assess their binding to LdHEL-67, a DDX3-DEAD box RNA helicase of <i>L.</i> donovani. Our results spotlighted nine major compounds with high binding energy, which were then further analyzed for ADMET properties and toxicity predictions, demonstrating their promising pharmacokinetic profiles. Among these, clionasterol emerged as the standout compound, displaying superior results in all in silico analyses compared to Amphotericin B (the control). Notably, clionasterol was present in significant proportions across all the mentioned extracts. Subsequent treatment with these extracts led to a remarkable reduction in the intracellular amastigote and axenic amastigote, and promastigote forms of <i>L. donovani</i> and non-toxic to THP-1-derived macrophages. Moreover, the extracts induced apoptotic effects, as evidenced by the fragmentation of parasitic genomic DNA. This study marks a significant leap in developing herbal-based, target-specific inhibitors against VL. Hence, our findings highlight the immense potential of <i>R. ellipticus</i> as a natural treatment for VL.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04183-4.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"18"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shen Zhi Ling oral liquid improve neuroinflammation against Alzheimer's disease via the PI3K/Akt pathway.","authors":"Gaofeng Qin, Weijuan Cui, Rongqiang Song","doi":"10.1007/s13205-024-04182-5","DOIUrl":"10.1007/s13205-024-04182-5","url":null,"abstract":"<p><p>The etiology and pathogenesis of Alzheimer's disease (AD) are complex, and currently, no comprehensive treatment measures exist. In this study, we initially utilized ultra-high-performance liquid chromatography with quadrupole orbitrap mass spectrometry (UHPLC-QE-MS) to profile the bioactive constituents of SZLOL present in the bloodstream. Subsequent Y-maze experimental data demonstrated that SZLOL could ameliorate short-term memory deficits in APP/PS1 mice. Furthermore, micro-positron emission tomography (Micro-PET) experiments revealed that SZLOL enhanced glucose metabolism in the cerebral cortex of the mice. To model AD in vitro, we utilized Aβ42-induced SH-SY5Y cells and assessed the effects of SZLOL-containing serum on cell growth and migration using immunofluorescence and wound-healing assays. Both in vivo and in vitro Western blot analyses indicated that SZLOL and SZLOL-containing serum were capable of activating the PI3K/Akt signaling pathway, which modulates the expression of inflammatory mediators. In future studies, we will validate our findings in more animal and cell models.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"29"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11695662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}