3 Biotech最新文献

{"title":"Algae-synthesized cerium oxide nanoparticles for antibiotic degradation in water and subsequent bioenergy production.","authors":"Monika Dubey, Jyoti Sharma, Richa Krishna, Vipin Chawla, Subhasha Nigam, Monika Joshi","doi":"10.1007/s13205-024-04134-z","DOIUrl":"https://doi.org/10.1007/s13205-024-04134-z","url":null,"abstract":"<p><p>In the present study, CeO₂ nanoparticles were synthesized using one-pot green route with high yield using microalgae <i>Chlorella sorokiniana.</i> The synthesized CeO₂ nanoparticles (CeO₂-np) exhibited rapid photocatalytic degradation 98.2% of doxycycline (DC) (20 mg/L) in only 30 min under visible light at pH7 in water. It was encouraging that CeO₂-np did not demonstrate a loss of photocatalytic activity up to five repeated cycles, confirming its stability during the degradation process. Moreover, cytotoxicity evaluation of CeO₂-nps on the green alga <i>Chlorella sorokiniana</i> advocated its non-toxic nature by supporting algal growth (0.52 g/L biomass<i>)</i> with 13% total lipids after 12 days in DC treated water. Ultimately, the produced algal biomass could be further utilized as a feedstock of biofuel production.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04134-z.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"318"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rational design of lanosterol 14α-demethylase for ergosterol biosynthesis in <i>Saccharomyces cerevisiae</i>.","authors":"Ruixue Liang, Kangjie Xu, Xinglong Wang, Wenqian Wei, Qihang Chen, Zhijie Qin, Weizhu Zeng, Jingwen Zhou","doi":"10.1007/s13205-024-04136-x","DOIUrl":"10.1007/s13205-024-04136-x","url":null,"abstract":"<p><p>Ergosterol is widely used in skin care products and drug preparation. Lanosterol 14α-demethylase (Erg11p, 14DM, CYP51) is the rate-limiting enzyme for the biosynthesis of various steroid compounds in <i>Saccharomyces cerevisiae</i>. Herein, Erg11p was engineered to extend the in vivo catalytic half-life and increase the turnover rate. Single mutations resulting in lower folding energy were selected, and mutant P201H had an ergosterol yield of 576.9 mg·L^-1. Through consensus design, single mutations resulting in higher sequence identity to homologs were tested and mutant K352L had an ergosterol yield of 677.9 mg·L^-1. The key residues for substrate binding were confirmed via alanine scanning mutagenesis and mutant F384A had an ergosterol yield of 657.8 mg·L^-1. Molecular dynamics (MD) simulation was conducted to investigate the contributions of pocket residues and eight residues were found to engage in weak interactions with lanosterol. Saturation mutagenesis was applied to these residues to enhance binding to lanosterol, and mutant F384E had an ergosterol yield of 733.8 mg·L^-1. Meanwhile, MD simulations were conducted to assess the impact of mutant F384E on enzyme activity. The results consistently showed that single point mutation F384E had the greatest effect, outperforming the combination mutations. Batch fermentation increased the ergosterol yield of mutant F384E to 3067.5 mg·L^-1, the highest reported to date. The successful engineering of Erg11p may pave the way for industrial-scale production of ergosterol and other steroids.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04136-x.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"300"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11564469/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the mechanism of carbamazepine decreasing testosterone levels based on cAMP/PKA/CREB pathway.","authors":"Jingya Li, Ziao Liu, Min Pan, Li Li, Xiaohui Tong, Yajuan Wang, Bin Chen, Tongsheng Wang","doi":"10.1007/s13205-024-04156-7","DOIUrl":"10.1007/s13205-024-04156-7","url":null,"abstract":"<p><p>The aim of this study was to explore the molecular mechanisms underlying carbamazepine (CBZ)-induced testicular toxicity and testosterone reduction in rats. For this purpose, Sprague-Dawley (SD) rats were intervened with 200 mg/kg CBZ for 12 weeks, and R2C cells were exposed to CBZ at concentrations of 0.5, 1 and 1.5 mM for 24 h. HE, Tunel, ELISA, immunofluorescence staining, RT-qPCR, and western blot were used to reveal the effects of CBZ on spermatozoa quality, testicular tissue structure, testosterone level and testosterone synthesis-related enzymes in rats. The results showed that CBZ significantly damaged the testicular tissue structure of rats, induced cell apoptosis, down-regulated the gene and protein expression levels of testosterone synthesis-related enzymes (STAR, TSPO, 17β-HSD and 3β-HSD), inhibited the expression of related proteins in the cAMP/PKA/CREB signalling pathway, and suppressed testosterone levels. In addition, the use of Db-cAMP (a PKA activator) significantly upregulated the protein expressions of PKA and p-CREB, evidently alleviated the CBZ-induced decrease in testosterone levels. In conclusion, CBZ induced testosterone resynthesis by inhibiting the cAMP/PKA/CREB pathway, affecting the expression of steroid synthesis-related enzymes and reducing testosterone levels.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"305"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11576689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142685744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"UPLC-QToF-MS/MS screening and characterization of <i>Symphorema polyandrum</i> Wight and in vitro assessment of its antioxidant, anticancer, and anti-inflammatory potential.","authors":"Dibya Ranjan Sahoo, Swaraj Kumar Babu, Baishali Basundhara Naik, Sajna Sameekshya Hota, Namita Bhoi, Barun Sarkar, S K Mustaq Ali, Pradeep Kumar Naik","doi":"10.1007/s13205-024-04144-x","DOIUrl":"10.1007/s13205-024-04144-x","url":null,"abstract":"<p><p><i>Symphorema polyandrum</i> belongs to the Lamiaceae family and is locally known as Badichang or mahasindhu. In this study, we performed Soxhlet extraction to prepare methanolic and hydromethanolic extracts, followed by quantification of their total phenolic content and total flavonoid content. Qualitative analysis of both the extracts was conducted to determine the presence of different phytochemicals. In addition, we aimed to identify the important phytochemical constituents in the methanolic extracts of <i>S. polyandrum</i> (SPM) using ultra-performance liquid chromatography hyphenated with high-resolution mass spectrometry (UPLC-ESI-QTOF-MS^E). Furthermore, this study investigated the antioxidant, anticancer and anti-inflammatory properties of SPM and its safety profile in the normal fibroblast cell line L929. A colony proliferation assay and a Griess assay were performed to evaluate the effects of SPM on colony formation and nitric oxide (NO) production. A total of 13 important phytochemicals were identified and reported. The methanolic extract of SPM demonstrated significant antioxidant activity. SPM also showed substantial antiproliferative activity on MDA-MB-231 triple-negative breast cancer cells, with an IC₅₀ value of 45.53 ± 1.63 µg/ml, and also reduced the survival of these cancer cells by promoting nuclear fragmentation and condensation without causing harm to normal cells. SPM inhibits the colony formation and reduces the nitric oxide (NO) production. The anti-inflammatory potential of SPM was assessed utilizing the murine alveolar macrophages (J774.A.1) as an in vitro model, and SPM effectively lowered the levels of proinflammatory cytokines such as TNF-α and IL-6. These findings emphasized the antiproliferative potential of SPM to cancer cells, along with its anti-inflammatory, and antioxidant capabilities, indicating the therapeutic efficacy of this medicinal plant.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"298"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557855/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomic characterization of the salt-tolerant <i>Bacillus</i> and <i>Jeotgalicoccus</i> strains reveals a diverse metabolism relevant to plant growth promotion and salt stress tolerance.","authors":"Sankalp Misra, Priti Prasad, Pradeep Semwal, Shashank Kumar Mishra, Mehar Hasan Asif, Puneet Singh Chauhan","doi":"10.1007/s13205-024-04164-7","DOIUrl":"https://doi.org/10.1007/s13205-024-04164-7","url":null,"abstract":"<p><p>Previous research leads have affirmed the crucial role of plant growth-promoting rhizobacteria (PGPR) strains <i>Bacillus safensis</i> (NBRI 12 M), <i>Bacillus subtilis</i> (NBRI 28B, NBRI 33N), and <i>Jeotgalicoccus huakuii</i> (NBRI 13E) in salt stress amelioration and plant growth promotion. In the present study, whole-genome analysis unveiled the underlying molecular mechanisms accounting for phyto-beneficial and stress-mitigating traits of the selected PGPR strains. The genomic characterization has revealed that NBRI 12 M, NBRI 28B, NBRI 33N, and NBRI 13E possessed a single circular chromosome of 3.73 Mb, 4.07 Mb, 4.10 Mb, and 2.17 Mb size, respectively. The genome analysis of these strains demonstrated varied genes such as mrp and yfiY for plant growth promotion, nutrient metabolism, and other secondary metabolites biosynthesis. High salinity tolerance genes (yicL, ydhP_1, spoIIQ, and spoIIID), encoding for membrane transporter, dormancy, and sporulation, were also identified. In addition, several chemotaxis (cheA, cheY, and cheW) and motility gene clusters (motB_1, motB_2) were found in the PGPR strains for successful rhizosphere colonization. Further, NBRI 12 M has significantly increased the shoot and root length and dry weight by 14.13%, 20.63%, and 9.63%, respectively, under salt stress. In addition, NBRI 12 M inoculation reduced defense enzymes by 79.77%, 84.75%, 74.11%, 70.77%, and 57.75% for SOD, APX, GPX, CAT, and PPO, respectively. Overall findings from this study offered a detailed comparative genomic analysis of salt stress ameliorating PGPR of <i>Bacillus</i> genera towards enhancing the deep insights for host-PGPR association.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04164-7.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"316"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604972/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Jumonji and AT-Rich Interacting Domain 2 (JARID2) exhibits a tumor-suppressive role in Oral Squamous Cell Carcinoma by modulating tumor progression and metastasis.","authors":"Bhuvanadas Sreeshma, A Mathan Mohan, Arikketh Devi","doi":"10.1007/s13205-024-04163-8","DOIUrl":"https://doi.org/10.1007/s13205-024-04163-8","url":null,"abstract":"<p><p>Jumonji and AT Rich Interacting Domain2 (JARID2), a pivotal accessory component of Polycomb Repressive Complex 2 (PRC2) is a critical factor in cancer development. The objective of the study was to determine the role of JARID2 in Oral Squamous Cell Carcinoma (OSCC). RT-PCR, qRT-PCR, immunofluorescence, immunohistochemistry, and western blot were used to analyze the gene and protein expression in OSCC clinical samples and OSCC cell lines. The experiments have collectively demonstrated the downregulation of JARID2 mRNA and protein expression during OSCC metastasis. The cytoplasmic localization of JARID2 in OSCC tissues and cell lines were also observed. In addition, JARID2 was knocked down in HSC-3 cells by performing siRNA-mediated transfection which revealed an increase in the expression of mesenchymal markers, N-cadherin and vimentin, and a downregulation of epithelial marker E-cadherin. Moreover, silencing JARID2 significantly increased the metastatic features such as migration, invasion, and colony-formation ability in HSC-3 cells. Also, the knockdown significantly reduced the number of apoptotic cells, suggesting that JARID2 knockdown has critically promoted HSC-3 cell metastasis by enhancing the mesenchymal markers. Taken together, the study has confirmed that JARID2 acts as a tumor suppressor, the downregulation of which promotes OSCC progression by regulating Epithelial-to-Mesenchymal Transition (EMT).</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04163-8.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"319"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11607193/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, radiolabeling, and biodistribution of 99 m-technetium-labeled zif-8 nanoparticles for targeted imaging applications.","authors":"Bandar Almutairy, Sitah Alharthi, Zyta M Ziora, Hasan Ebrahimi Shahmabadi","doi":"10.1007/s13205-024-04145-w","DOIUrl":"https://doi.org/10.1007/s13205-024-04145-w","url":null,"abstract":"<p><p>This study investigates the synthesis and radiolabeling of zeolitic imidazolate frameworks (ZIF-8) with the radioisotope technetium-99 m (^99mTc) using a solvothermal method in methanol. The methanolic medium facilitated the formation of nanoparticles with favorable characteristics, including a smaller particle size (198 ± 9.8 nm) and a low polydispersity index (PDI = 0.219 ± 0.011). Radiolabeling efficiency (RE%) and radiochemical purity (RCP%) were optimized by employing SnCl₂ as a reducing agent, resulting in an RE% of 95.2 ± 1.9% and an RCP% of 96.1 ± 1.7% in triplicate (<i>n</i> = 3) at 65 °C. The nanoparticles exhibited high serum stability, retaining 99.05% of RCP% after 24 h, and demonstrated hemocompatibility, with hemolysis rates below 5% across all tested concentrations. In vitro biocompatibility assessments using NIH-3T3 cells indicated cell viability above 70% at concentrations up to 40 μg/mL. Biodistribution studies in rabbits (<i>n</i> = 6) revealed predominant accumulation in the bladder, with radiotracer uptake in the bladder being 6.3, 7.2, and 36.2 times higher than in the liver, kidneys, and heart (<i>p</i> < 0.0001), respectively, suggesting renal clearance. These results underscore the potential of ^99mTc-(ZIF-8) nanoparticles for biomedical applications, particularly in targeted imaging and drug delivery. Future research will focus on improving targeting specificity and enhancing therapeutic efficacy in disease models.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-024-04145-w.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"293"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11549264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parathyroid allotransplantation for severe post-surgical hypoparathyroidism: a Brazilian experience.","authors":"Daniel Duarte Gadelha, Wellington Alves Filho, Catarina Brasil d'Alva, Tainá Veras de Sandes-Freitas, Renan Magalhães Montenegro Júnior","doi":"10.1007/s13205-024-04147-8","DOIUrl":"10.1007/s13205-024-04147-8","url":null,"abstract":"<p><p>This study presents the cases of two women who developed severe permanent hypoparathyroidism after neck surgery for papillary thyroid cancer and underwent parathyroid allotransplantation. Despite taking high doses of calcium and calcitriol supplements, the patients experienced persistent hypocalcemic symptoms. Fresh parathyroid tissue was removed and prepared from two patients with hyperparathyroidism secondary to end-stage kidney disease and was implanted in the non-dominant forearm of the recipients. Donors and recipients were ABO-compatible, and immunological screening was performed only in Case 2 (HLA typing, panel reactive antibody, and crossmatch tests). A short-term immunosuppressive regimen was adopted, consisting of 3 days of methylprednisolone followed by 7 days of prednisone. In Case 1, oral supplementation decreased to half of the initial dose 1 month after transplantation and to one-fifth at the end of a 12-month follow-up period. In Case 2, intravenous calcium was discontinued 1-week post-transplantation, with no need for its use during the 12-month follow-up period. Serum parathyroid hormone levels did not increase and remained undetectable in both cases. In contrast, serum calcium levels increased significantly, and both patients experienced relief from hypocalcemic symptoms. Parathyroid allotransplantation can be an effective and safe treatment for PH and should be considered in severe cases. Nevertheless, formal recommendations depend on additional studies and validated protocols.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"299"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11564487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microencapsulation and nanoencapsulation of bacterial probiotics: new frontiers in Alzheimer's disease treatment.","authors":"M Lavanya, S Karthick Raja Namasivayam, S Priyanka, T Abiraamavalli","doi":"10.1007/s13205-024-04158-5","DOIUrl":"10.1007/s13205-024-04158-5","url":null,"abstract":"<p><p>Alzheimer's disease, a progressive neurodegenerative disorder marked by cognitive decline, affects millions worldwide. The presence of amyloid plaques and neurofibrillary tangles in the brain is the key pathological feature, leading to neuronal dysfunction and cell death. Current treatment options include pharmacological approaches such as cholinesterase inhibitors, as well as non-pharmacological strategies like cognitive training and lifestyle modifications. Recently, the potential role of probiotics, particularly strains, such as <i>Lactobacillus</i> and <i>Bifidobacterium</i>, in managing neurodegenerative diseases through the gut-brain axis has garnered significant attention. Probiotics can modulate inflammation, produce neurotransmitters, and support neuronal health, potentially slowing disease progression and alleviating symptoms, such as stress and anxiety. Optimizing the pharmacotherapeutic effects of probiotics is critical and involves advanced formulation techniques, such as microencapsulation and nanoencapsulation. Microencapsulation employs natural or synthetic polymers to protect probiotic cells, enhancing their viability and stability against environmental stressors. Methods like extrusion, emulsion, and spray-drying are used to create microcapsules suited for various applications. Nanoencapsulation, on the other hand, operates at the nanoscale, utilizing polymeric or lipid-based nanoparticles to improve the bioavailability and shelf life of probiotics. Techniques, such as nanoprecipitation and emulsification, are employed to ensure stable nanocapsule formation, thereby augmenting the therapeutic potential of probiotics as nutraceutical agents. This study delves into the essential formulation aspects of microencapsulation and nanoencapsulation for beneficial probiotic strains, aimed at managing Alzheimer's disease by optimizing the gut-brain axis. The insights gained from these advanced techniques promise to enhance probiotic delivery efficacy, potentially leading to improved health outcomes for patients suffering from neurodegenerative disorders.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"313"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599650/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increasing pollination possibilities in <i>Paspalum</i> species: in vitro and in vivo viability of cryopreserved pollen to address flowering asynchrony.","authors":"Naiana Barbosa Dinato, Bianca Baccili Zanotto Vigna, Frederico de Matta, Alessandra Pereira Fávero","doi":"10.1007/s13205-024-04138-9","DOIUrl":"10.1007/s13205-024-04138-9","url":null,"abstract":"<p><p><i>Paspalum</i> is a vital forage and turf grass in tropical and subtropical regions, yet its breeding programs face challenges due to the lack of natural flowering synchronization between some parent species. Pollen cryopreservation offers a potential solution to this issue. This study aimed to adapt a cryopreservation protocol for the pollen of <i>P. atratum</i>, <i>P. malacophyllum</i>, and <i>P. regnellii</i>, and to evaluate the viability of cryopreserved pollen grains (CPG) for hybridization purposes. Two dehydrating agents (LiCl and silica gel) were tested for different durations (30, 60, and 120 min) alongside a non-dehydration treatment. The effectiveness of cryopreservation was assessed over multiple time points (1, 10, 30, 90, 180, 270, and 365 days) with freshly harvested grains as controls. Pollen viability was determined using 0.25% 2,3,5-triphenyltetrazolium chloride staining. Viability of CPG ranged from 40.67 to 80.67% across treatments. Optimal dehydration involved LiCl for 30 min and silica gel for 120 min, achieving an average viability of 66% after 12 months, comparable to fresh pollen. In vivo germination tests confirmed successful pollen tube germination with the combinations <i>P. urvillei</i> × <i>P. malacophyllum</i>; <i>P. urvillei</i> × <i>P. regnellii</i> and hybrid (<i>P. plicatulum</i> 4PT × <i>P. guenoarum</i> cv. Azulão) × <i>P. atratum</i>, although pollen tubes did not reach the micropyle in some crosses. This study established effective pollen cryopreservation protocols for <i>P. atratum</i> and <i>P. malacophyllum</i>, facilitating in vivo germination and enhancing the potential for hybridization in <i>Paspalum</i> breeding programs, thereby addressing flowering asynchrony and broadening crossing opportunities within the genus.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"308"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11582290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142708961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}