3 Biotech最新文献

{"title":"Analytical assessment of safety and efficacy of filgrastim and pegfilgrastim formulations for treatment of chemotherapy-induced neutropenia.","authors":"Nupur Garg, Birendra Kumar, Sanjay Mendiratta, Gurminder Bindra, Poonam Katoch, Mohit Lal, Priyanka Gupta, Jyoti Arya, Harish Chander, Charu Mehra Kamal","doi":"10.1007/s13205-025-04457-5","DOIUrl":"https://doi.org/10.1007/s13205-025-04457-5","url":null,"abstract":"<p><p>A recombinant preparation of human granulocyte-colony stimulating factors (rh-GCSF), filgrastim and its advanced form pegfilgrastim i.e., pegylated form have widely been employed in the management of neutropenia caused by cancer chemotherapy. Their formulations from different manufacturers are commercially available in the market for patient use. Though regulatory guidelines worldwide have laid down methodology for ensuring quality control testing of such products before entering into the market, yet, the quality data of copy products are seldom available. In this 8 year retrospective analysis carried out on seven products each of both the molecules, a comparison of quality parameters based on their safety and efficacy studies is presented to understand any variability. The utilization of orthogonal techniques was done for the filgrastim and pegfilgrastim products of different manufacturers to address the critical quality attributes for identity, purity, potency and bioactivity. The quality of the batches was analyzed based on compendial guidelines for filgrastim products and manufacturers' method of analysis for pegfilgrastim products. The products were evaluated for presence of endotoxin, aggregate impurities (monomer > 97%), related proteins (< 3.5%), and other impurities with different molecular masses (no separate band in SDS-PAGE) and were found within limits. The products were also assessed for their potency though cell-based proliferation assay (80-125%) and protein content (80-125%). They were found to be of standard quality with no substantial differences in quality parameters among batches of a product (intra-variability) and among all different products (intervariability).</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 9","pages":"283"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12328858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144815479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>CPNE2</i> might serve as a new negative prognostic biomarker in gastric cancer.","authors":"Hasan Çağrı Yıldırım, Fatih Yay, Gökhan Şahin, Fatih Kuş, Şuayib Yalçın","doi":"10.1007/s13205-025-04491-3","DOIUrl":"10.1007/s13205-025-04491-3","url":null,"abstract":"<p><p>Gastric cancer is a common malignancy with a high mortality rate. Improvement in survival has been observed with therapies targeting HER2 and Claudin 18.2 or with immune checkpoint inhibitors. Despite these advancements, the prognosis remains poor. Therefore, there is a need to identify new prognostic biomarkers that may play a role in gastric cancer and then conduct studies that can target these molecules. In this study, bioinformatics databases were utilized to analyze <i>CPNE2</i> gene expression in normal and tumor samples, and its association with cancer stage was investigated. The relationship between <i>CPNE2</i> gene expression and Overall Survival (OS) was analyzed. <i>CPNE2</i> gene expression was significantly higher in gastric tumor samples compared to normal tissues (<i>p</i> < 0.05). Higher <i>CPNE2</i> expression was observed in stages 2, 3, and 4 compared to stage 1. Elevated <i>CPNE2</i> gene expression was associated with shorter mOS in both the GeneChip Gastric Cancer and RNAseq STAD databases. In the OS analysis using the GeneChip database, the hazard ratio (HR) was 1.72 (1.37-2.16), <i>p</i> = 1.8e-6, with a false discovery rate (FDR) of 1%, and a cutoff value of 311. High CPNE2 gene expression was associated with shorter FP (HR = 1.85 (1.36-2.51), <i>P</i> = 6e-05, FDR = 2%, cutoff value = 216). In our study, higher CPNE2 gene expression was detected in advanced-stage patients with gastric cancer compared to early-stage patients, and high CPNE2 expression was found to be associated with poor survival.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04491-3.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 9","pages":"319"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12397100/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocompatible synthesis, computational studies and therapeutic evaluation of a dibutyl (2,2-dicyano-1-phenylethyl) phosphonate derivatives targeting oxidative stress, microbial infection and cancer cells.","authors":"Sumithra Poreddy, Santhisudha Sarva, Surendra Pothuraju, Mohan Gundluru, Poojitha Bellala, Sunitha Gundubogula, Suresh Reddy Cirandur","doi":"10.1007/s13205-025-04499-9","DOIUrl":"10.1007/s13205-025-04499-9","url":null,"abstract":"<p><p>We synthesized a series of dibutyl (2,2-dicyano-1-phenylethyl) phosphonates using a solvent-free, one-pot reaction catalysed by L-carnitine hydroxide. This green method yielded products in 75-97% yield. Density functional theory analysis showed that these compounds have favourable HOMO-LUMO gaps and enhanced charge transfer, suggesting good nonlinear optical properties. Molecular docking indicated strong binding to key bacterial proteins. In vitro tests confirmed strong antioxidant activity (DPPH IC₅₀: 21.1-49.8 µg/mL; NO IC₅₀: 21.2-31.1 µg/mL) and effective antibacterial action against both Gram-positive and Gram-negative bacteria (inhibition zones: 2.0-3.8 mm). The compounds also inhibited cancer cell growth in five human cell lines (IC₅₀: 3.5-5.9 µM), with higher selectivity than doxorubicin over non-cancerous cells. ADMET analysis predicted good absorption, low toxicity and drug-likeness. Electron-withdrawing groups improved both biological and electronic properties. These phosphonates are promising candidates for new therapeutic development.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04499-9.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 9","pages":"321"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12398457/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering morpho-physiochemical diversity, mineral profiling, and population structure in brinjal (<i>Solanum melongena</i> L.) germplasm.","authors":"Akanksha Tripathi, Mihir M Pandya, Dileep Kumar, Rumit Patel, Amar A Sakure, Prity Kumari, Sushil Kumar","doi":"10.1007/s13205-025-04487-z","DOIUrl":"10.1007/s13205-025-04487-z","url":null,"abstract":"<p><p>Brinjal (<i>Solanum melongena</i> L.) is a key solanaceous vegetable in Asian and African countries, rich in minerals and vitamins in tropical diets. This study evaluated the variability and genetic diversity of 94 genotypes grown in an Augmented Randomized Complete Block Design during <i>kharif-rabi</i> (monsoon-winter) 2023-24. Analysis of variance revealed significant differences among genotypes for all traits, except total soluble solids, indicating substantial genetic variability, which was further supported by variability parameters showing considerable variation among the genotypes for all studied traits. No negative correlations were observed between yield or fruit weight and the minerals traits. First five principal components explained 62.83% of the cumulative variation. The clustering based on the Manhattan dissimilarity coefficient for quantitative traits divided 94 genotypes into eight clusters. Genetic diversity assessment employed simple sequence repeats (SSR)/microsatellites markers, revealing 63 distinct alleles across 15 primers with variable band sizes and polymorphic information content (PIC) varied from 0.2854 (EM 120) to 0.6748 (CSM31). The dendrogram constructed from SSR markers categorized the genotypes into three main clusters (A, B, and C), with further sub-clustering revealing genetic similarities among genotypes. The population structure analysis using the STRUCTURE software classified genotypes into four groups (G1-G4) based on SSR markers. Sub-population G4 exhibited the highest genetic diversity. Fruit yield per plant, average fruit weight, days to 50% flowering, iron content, and potassium content emerged as significant contributors to diversity. The findings highlight the potential for hybridization to improve yield and the use of diverse genotypes as parents in bi-parental mating and mapping studies to enhance nutrient content through targeted breeding.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04487-z.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 9","pages":"313"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12378805/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilizing meta-topolin for the micropropagation of high-yielding natural clones of <i>Centella asiatica</i> (L.) Urban and evaluation of their anti-uropathogenic activity.","authors":"Suproteem Mukherjee, Avijit Chakraborty, Indranil Santra, Biswajit Ghosh","doi":"10.1007/s13205-025-04477-1","DOIUrl":"10.1007/s13205-025-04477-1","url":null,"abstract":"<p><p><i>Centella asiatica</i> (L.) Urb., also known as Indian pennywort, is an important ethnomedicinal herb valued for its bioactive compounds, including asiaticoside, madecassoside, asiatic acid, and madecassic acid, collectively termed centellosides, which possess neuroprotective, wound-healing, and antioxidant properties<i>.</i> In the present study, 87 accessions of <i>C. asiatica</i> were collected from six agro-climatic zones of West Bengal, India, and evaluated for centelloside content and bioactivity. The chemotype C20 was identified as a high-yielding chemotype, exhibiting maximum accumulation of secondary metabolites: 31.02 ± 0.29 mg/g asiaticoside, 37.53 ± 0.11 mg/g madecassoside, 16.84 ± 0.06 mg/g asiatic acid, and 14.16 ± 0.12 mg/g madecassic acid, as quantified by high-performance thin layer chromatography. This chemotype also demonstrated the highest antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl assay, 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid assay, and ferric reducing antioxidant power assay. This chemotype has also shown significant antimicrobial potential, with a maximum inhibition zone of 18.33 ± 0.26 mm against the human pathogen <i>Staphylococcus aureus</i>. Due to the optimum efficiency of C20 in all aspects studied in the present findings, among the 87 accessions, it is considered an elite chemotype. Micropropagation of elite chemotype using MS medium supplemented with <i>meta</i>-topolin (1.5 mg/L) and indole-3-acetic acid (0.5 mg/L) yielded maximum (28.39 ± 0.06) shoots per explant, while optimal rooting was achieved on half-strength MS medium with 0.5 mg/L indole-3-butyric acid. Genetic and phytochemical fidelity between wild and regenerated plants was confirmed through SCoT marker profiling and high-performance liquid chromatography. This reproducible method for elite plant selection and their mass production has the potential to revolutionise the pharmaceutical and herbal medicine industries.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 9","pages":"317"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12394101/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144938008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytotherapeutic evaluation of rosehip seed and olive oils on wound healing: evidence from L929 cell culture and in vivo VEGF/TGF-β analysis.","authors":"Cigdem Aydin Acar, Funda Gul Kiyak, Suray Pehlivanoglu, Sukriye Yesilot, Ozlem Ozmen","doi":"10.1007/s13205-025-04498-w","DOIUrl":"10.1007/s13205-025-04498-w","url":null,"abstract":"<p><p>This study aimed to evaluate and compare the wound healing efficacy of rosehip seed oil and olive oil using both in vitro and in vivo models. The novelty of this work lies in the comparative investigation of these two natural oils with documented antioxidant profiles, focusing on their regenerative capacity. Gas chromatography-mass spectrometry (GC-MS) analysis revealed linoleic acid (45.46%) as the major component of rosehip seed oil and oleic acid (78.16%) as dominant in olive oil. In vitro 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT) assays on L929 fibroblasts confirmed the nontoxic nature of both oils at low concentrations. In vitro scratch assays showed that the wound closure rate increased from 85.22% in the control group to 96.49% with 0.1% rosehip seed oil and 92.67% with 0.01% olive oil. The combination of oils achieved a 91.72% closure rate. In vivo, oils were topically applied to rat excisional wounds for 10 days. The negative control group had a 52.97% healing rate, while rosehip seed oil treatment improved it to 69.13%, when compared with 57.13% with olive oil and 60.43% with the oil mixture. Histopathological analysis showed significant epithelial tissue regeneration with rosehip seed oil. This group also demonstrated the highest vascular endothelial growth factor (VEGF) and transforming growth factor-β (TGF-β) expression levels, greater total antioxidant capacity, and the most effective oxidative stress reduction. These results suggest that rosehip seed oil may be a potent natural agent for promoting wound repair, likely due to its antioxidant and anti-inflammatory properties. However, further studies involving additional cell lines, prolonged treatment durations, and clinical validation are warranted to confirm its therapeutic potential.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 9","pages":"324"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12399505/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144991267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of engineered <i>Saccharomyces cerevisiae</i> for producing phytosphingosine.","authors":"Siyan Qiu, Jingru Li, Pengtian Xie, Chun Wei, Jie Sun","doi":"10.1007/s13205-025-04417-z","DOIUrl":"https://doi.org/10.1007/s13205-025-04417-z","url":null,"abstract":"<p><p>Phytosphingosine (PHS), a sphingolipid-derived bioactive compound, exhibits multifunctional properties including antimicrobial activity, skin moisturization, and hydration, rendering it highly valuable for cosmetic and pharmaceutical applications. Through systematic metabolic engineering of <i>Saccharomyces cerevisiae</i>, we achieved 82.62 mg/g DCW phytosphingosine (PHS) production via integrated pathway optimization and stress mitigation. Key strategies involved: (1) Knockout of <i>LCB4</i> (sphingoid long-chain base kinase), <i>SHM2</i> (serine hydroxymethyltransferase), and <i>CHA1</i> (l-serine deaminase) to block competitive pathways; (2) Overexpression of <i>TSC10</i> (3-ketosphingosine reductase), <i>SYR2</i> (sphingosine hydroxylase), and <i>LCB1/LCB2</i> (serine palmitoyltransferase) to amplify the PHS synthesis flux. Initial shake flask fermentation (96 h) yielded 15.31 mg/g DCW of PHS, with <i>ORM2</i> knockout providing a 73.6% productivity increase (26.54 mg/g DCW) despite inducing growth defects from sphingosine accumulation. We hypothesized that disrupted ORM2-mediated control of serine palmitoyltransferase activity might compromise ER homeostasis through sphingolipid imbalance, which was alleviated through <i>HAC1</i> overexpression to enhance unfolded protein response (UPR) capacity. Fed-batch fermentation under optimized conditions (40 mM serine, 0.5 mM palmitic acid, pH 5) demonstrated scalable production, delivering a 5.4-fold improvement over baseline. This work establishes UPR engineering as a critical strategy for resolving lipid toxicity constraints in yeast sphingolipid biosynthesis, while highlighting <i>S. cerevisiae</i>'s potential as an industrial PHS production platform through coordinated pathway and stress response manipulation.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04417-z.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"247"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12238450/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144607095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of mechanism of anticancer potential of fisetin nanoformulations: nanotized fisetin/fisetin loaded polymannose nanoparticles in the treatment of triple negative breast carcinoma.","authors":"Neena Yadav, Saswat Kumar Mohanty, Sathyapriya Chandramohan, V K Archana, Kitlangki Suchiang, Rukkumani Rajagopalan","doi":"10.1007/s13205-025-04427-x","DOIUrl":"10.1007/s13205-025-04427-x","url":null,"abstract":"<p><p>Triple-negative breast cancer (TNBC) remains one of the most aggressive and difficult-to-treat subtypes of breast cancer due to the lack of targeted therapies. This study investigates the anti-proliferative and anti-metastatic potential of fisetin (FIS) and its nanoformulations, nanotized fisetin (Nano-FIS) and FIS-loaded polymannose nanoparticles (FIS-PM NPs) in TNBC cells. Both Nano-FIS and FIS-PM NPs demonstrated significant, time- and dose-dependent cytotoxicity, with FIS-PM NPs showing the greatest inhibition of cell viability. Morphological assessments revealed pronounced apoptotic features, including cell shrinkage and rounding, particularly in nanoformulation-treated cells. In vitro wound healing assays confirmed that Nano-FIS and FIS-PM NPs markedly delayed cell migration compared to free FIS, indicating strong anti-metastatic properties. Western blot analysis further revealed that treatment with the nanoformulations downregulated pro-survival proteins NF-κB p65, Cyclin D1, and Bcl-2, while upregulating pro-apoptotic proteins Caspase-3 and Caspase-8, with FIS-PM NPs eliciting the most pronounced effects. RT-PCR analysis supported these findings by demonstrating significant downregulation of angiogenesis- and metastasis-related genes VEGF-A, VCAM1, MMP-9, and MMP-2 in nanoformulation-treated TNBC cells. Overall, the results suggest that FIS-based nanoformulations, particularly FIS-PM NPs, exhibit enhanced anti-cancer efficacy through the induction of apoptosis, inhibition of cell proliferation, migration, and angiogenesis, making them promising therapeutic candidates for TNBC treatment.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04427-x.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"267"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12290157/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lumefantrine-doped cerium oxide nanoparticles: investigating their antibacterial and mosquito larvicidal properties.","authors":"Udaiyan Suresh, Karuppaiya Deepajothika, Sivaperumal Sivaramakrishnan, Chellasamy Panneerselvam, Ahmed Saif, Mohammed Ali Alshehri, Ayed A Dera","doi":"10.1007/s13205-025-04429-9","DOIUrl":"10.1007/s13205-025-04429-9","url":null,"abstract":"<p><p>Silver-doped cerium oxide nanoparticles (Ag-doped CeO₂ NPs) were synthesized using lumefantrine as a capping agent and characterized using UV-Vis, TEM, SEM, EDX, XRD, FTIR, and zeta potential analyses. The nanoparticles exhibited strong larvicidal activity against <i>Aedes</i> <i>aegypti</i>, with LC₅₀ values of 10.432 µg/mL (third instar) and 12.479 µg/mL (fourth instar), and LC₉₀ values of 23.016 µg/mL and 26.455 µg/mL, respectively. In comparison, lumefantrine alone showed LC₅₀ values of 64.973 µg/mL and 83.182 µg/mL for the third and fourth instars. Egg hatchability, adult longevity, and fecundity were significantly reduced following treatment with the nanoparticles. Microscopic observations revealed severe morphological damage in treated larvae, including deformities in the abdomen, tracheal gills, cuticle, thorax, epithelial layer, and midgut. The Ag-doped CeO₂ NPs also exhibited antibacterial activity against <i>Klebsiella pneumoniae</i>, <i>Bacillus subtilis</i>, <i>Escherichia coli</i>, and <i>Streptococcus aureus</i>. These findings confirm the multifunctionality of Ag-doped CeO₂ NPs as potent larvicidal and antibacterial agents with potential application in vector control and microbial management.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"271"},"PeriodicalIF":2.9,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12297186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the prognostic signature of nonsmall cell lung cancer using cisplatin resistance and circulating tumor cell-related gene analysis.","authors":"Linlu Gao, Xiaoyuan Sun, Lei Wang, Kun Gao, Lianyang Yu, Yanying Wang","doi":"10.1007/s13205-025-04402-6","DOIUrl":"https://doi.org/10.1007/s13205-025-04402-6","url":null,"abstract":"<p><p>This study aimed to develop a prognostic prediction model for nonsmall cell lung cancer (NSCLC) based on cisplatin resistance and circulating tumor cell (CTC)-related genes and to explore the molecular mechanisms of key genes. After downloading and preprocessing data from The Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) databases, a prognostic signature was developed using least absolute shrinkage and selection operator (LASSO) regression analysis. Patients were categorized into low-risk (LR) and high-risk (HR) groups based on their risk score (RS), and differences in survival, immune infiltration, and immunotherapy/chemotherapy responses were compared. A nomogram was established for clinical application, followed by identification of molecular subtypes. Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) was used to verify the expression of key genes in the A549 and A549/DDP cell lines. Finally, we explored the molecular mechanisms by which inhibition of FAM83A reversed cisplatin resistance. Using LASSO regression, a prognostic signature with eight genes was developed; the survival rate of patients in the HR group was significantly lower than that of patients in the LR group (all <i>P</i> < 0.05). The RS was an independent risk factor for NSCLC and stably predicted the prognosis (<i>P</i> < 0.05). The immune infiltration levels of LR and HR patients were significantly different, and the model could effectively predict the response to drugs or immunotherapy (all <i>P</i> < 0.05). Subsequently, subtype clustering divided the patients into two groups to assist in identifying heterogeneity between patients with different risks. Finally, qRT-PCR confirmed that these genes were aberrantly expressed in A549 cells and were closely associated with cisplatin resistance (all <i>P</i> < 0.05). Notably, FAM83A overexpression promoted the proliferation and invasion and inhibited the apoptosis of A549 cells (all <i>P</i> < 0.05). Low FAM83A expression reversed cisplatin resistance in lung cancer cells (<i>P</i> < 0.05). The eight-gene model constructed in this study can predict the prognosis of patients with NSCLC and guide personalized treatment. In addition, targeted inhibition of FAM83A expression can reverse cisplatin resistance, potentially enhancing the efficacy of chemotherapy in combination with immunotherapy, thereby providing a novel strategy for clinical practice.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 8","pages":"259"},"PeriodicalIF":2.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12271037/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144673693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}