Utilizing meta-topolin for the micropropagation of high-yielding natural clones of Centella asiatica (L.) Urban and evaluation of their anti-uropathogenic activity.

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-09-01 Epub Date: 2025-08-28 DOI:10.1007/s13205-025-04477-1

Suproteem Mukherjee, Avijit Chakraborty, Indranil Santra, Biswajit Ghosh

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引用次数: 0

Abstract

Centella asiatica (L.) Urb., also known as Indian pennywort, is an important ethnomedicinal herb valued for its bioactive compounds, including asiaticoside, madecassoside, asiatic acid, and madecassic acid, collectively termed centellosides, which possess neuroprotective, wound-healing, and antioxidant properties. In the present study, 87 accessions of C. asiatica were collected from six agro-climatic zones of West Bengal, India, and evaluated for centelloside content and bioactivity. The chemotype C20 was identified as a high-yielding chemotype, exhibiting maximum accumulation of secondary metabolites: 31.02 ± 0.29 mg/g asiaticoside, 37.53 ± 0.11 mg/g madecassoside, 16.84 ± 0.06 mg/g asiatic acid, and 14.16 ± 0.12 mg/g madecassic acid, as quantified by high-performance thin layer chromatography. This chemotype also demonstrated the highest antioxidant activity in the 2,2-diphenyl-1-picrylhydrazyl assay, 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid assay, and ferric reducing antioxidant power assay. This chemotype has also shown significant antimicrobial potential, with a maximum inhibition zone of 18.33 ± 0.26 mm against the human pathogen Staphylococcus aureus. Due to the optimum efficiency of C20 in all aspects studied in the present findings, among the 87 accessions, it is considered an elite chemotype. Micropropagation of elite chemotype using MS medium supplemented with meta-topolin (1.5 mg/L) and indole-3-acetic acid (0.5 mg/L) yielded maximum (28.39 ± 0.06) shoots per explant, while optimal rooting was achieved on half-strength MS medium with 0.5 mg/L indole-3-butyric acid. Genetic and phytochemical fidelity between wild and regenerated plants was confirmed through SCoT marker profiling and high-performance liquid chromatography. This reproducible method for elite plant selection and their mass production has the potential to revolutionise the pharmaceutical and herbal medicine industries.

查看原文本刊更多论文

利用元topolin进行积雪草（Centella asiatica， L.）高产自然无性系的微繁它们的抗尿路致病活性及其评价。

积雪草（Centella asiatica）市区。它是一种重要的民族草药，因其生物活性化合物而受到重视，包括积雪草苷、马钱子苷、积雪草酸和马钱子酸，统称为积雪草苷，具有神经保护、伤口愈合和抗氧化特性。本研究从印度西孟加拉邦6个农业气候带采集了87份亚洲桤木，对其centello苷含量和生物活性进行了评价。C20化学型为高产化学型，其次生代谢产物积累量最高：高效薄层色谱法测定其累积量分别为：31.02±0.29 mg/g积雪草苷、37.53±0.11 mg/g积雪草苷、16.84±0.06 mg/g积雪草酸和14.16±0.12 mg/g积雪草酸。该化学型在2,2-二苯基-1-吡啶酰肼、2,2'-氮唑-3-乙基苯并噻唑-6-磺酸和铁还原抗氧化能力试验中也显示出最高的抗氧化活性。该化学型对人类金黄色葡萄球菌的最大抑菌区为18.33±0.26 mm。由于C20在本研究各方面的效率最优，在87份材料中被认为是精英化学型。在MS培养基中添加间topolin （1.5 mg/L）和吲哚-3-乙酸（0.5 mg/L），每个外植体可获得最多（28.39±0.06）个芽，而在半强度MS培养基中添加0.5 mg/L的吲哚-3-丁酸，可获得最佳生根效果。通过SCoT标记分析和高效液相色谱分析，证实了野生和再生植株的遗传和植物化学保真度。这种可重复的方法用于精英植物的选择和大规模生产，有可能彻底改变制药和草药行业。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.