3 Biotech最新文献_第9页

c-MET tyrosine kinase inhibitors reverse drug resistance mediated by the ATP-binding cassette transporter B1 (ABCB1) in cancer cells. c-MET酪氨酸激酶抑制剂逆转由atp结合盒转运蛋白B1 （ABCB1）介导的癌细胞耐药。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-04 DOI: 10.1007/s13205-024-04162-9

Somayeh Nazari, Alireza Poustforoosh, Priyanka Rani Paul, Ritushree Kukreti, Marjan Tavakkoli, Luciano Saso, Omidreza Firuzi, Fatemeh Moosavi

{"title":"c-MET tyrosine kinase inhibitors reverse drug resistance mediated by the ATP-binding cassette transporter B1 (ABCB1) in cancer cells.","authors":"Somayeh Nazari, Alireza Poustforoosh, Priyanka Rani Paul, Ritushree Kukreti, Marjan Tavakkoli, Luciano Saso, Omidreza Firuzi, Fatemeh Moosavi","doi":"10.1007/s13205-024-04162-9","DOIUrl":"10.1007/s13205-024-04162-9","url":null,"abstract":"This study investigated the potential of MET kinase inhibitors, cabozantinib, crizotinib, and PHA665752, in reversing multidrug resistance (MDR) mediated by ABCB1 in cancer cells. The accumulation of the fluorescent probe, Rhodamine 123, was assessed using flow cytometry and fluorescence microscopy in MDR MES-SA/DX5 and parental cells. The growth inhibitory activity of MET inhibitors as monotherapies and in combination with chemotherapeutic drugs was evaluated by MTT assay. CalcuSyn software was used to analyze the combination index (CI) as an index of drug-drug interaction in combination treatments. Results showed that at concentrations of 5, and 25 μM, c-MET inhibitors significantly increased Rhodamine 123 accumulation in MDR cells, with ratios up to 17.8 compared to control cells, while exhibiting no effect in parental cells. Additionally, the combination of c-MET inhibitors with the chemotherapeutic agent doxorubicin synergistically enhanced cytotoxicity in MDR cells, as evidenced by combination index (CI) values of 0.54 ± 0.08, 0.69 ± 0.1, and 0.85 ± 0.07 for cabozantinib, crizotinib, and PHA665752, respectively. While all three c-MET inhibitors stimulated ABCB1 ATPase activity in different manners at certain concentrations, PHA-665752 suppressed it at high concentration. In silico analysis also suggested that the transmembrane domains (TMD) of ABCB1 transporters could be considered potential target for these agents. Our results suggest that c-MET inhibitors can serve as promising MDR reversal agents in ABCB1-medicated drug-resistant cancer cells.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"2"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11618280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142798942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppression of KLF5 targets RREB1 to restrain the proliferation of ovarian cancer cells through ERK/MAPK signaling pathway. 抑制KLF5通过ERK/MAPK信号通路靶向RREB1抑制卵巢癌细胞增殖。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-11 DOI: 10.1007/s13205-024-04171-8

Shenglan Wang, Chuanchuan Liu, Yongchuan Li, Jinwan Qiao, Xinling Chen, Jin Bao, Ran Li, Yanxia Xing

{"title":"Suppression of KLF5 targets RREB1 to restrain the proliferation of ovarian cancer cells through ERK/MAPK signaling pathway.","authors":"Shenglan Wang, Chuanchuan Liu, Yongchuan Li, Jinwan Qiao, Xinling Chen, Jin Bao, Ran Li, Yanxia Xing","doi":"10.1007/s13205-024-04171-8","DOIUrl":"10.1007/s13205-024-04171-8","url":null,"abstract":"The overexpression of Kruppel-like factor 5 (KLF5) appears in several types of cancer. KLF5 may be an effective therapeutic target for treating OC, but its function in ovarian cancer (OC) remains unknown. The KLF5 mRNA expression levels in several OC cell lines were analyzed using RT-qPCR. Then, NC-siRNA or KLF5-siRNA was transfected into SK-OV-3 and OVCAR-3 cells. RT-qPCR and WB were used to detect the efficiency of KLF5 silence, CCK-8, colony formation assay, IHC staining, flow cytometry, and WB were performed to investigate the KLF5 function on OC cell proliferation and the activation of the extracellular signal-regulated Kinase (ERK)/mitogen-activated protein kinase (MAPK) signaling pathway. Next, a dual-luciferase and IF assay were used to determine the relationship between KLF5 and the Ras response element-binding protein (RREB1). SK-OV-3 and OVCAR-3 cells were treated with KLF5-siRNA and C16-PAF + EGF (MAPK agonist), separately or in combination. Proteins including KLF5, RREB1, p-p38, p-ERK1/2, ERK5, p-ERK5, Cyclin D1, CDK4, and CDK6 were quantified by WB. Finally, CCK-8, colony formation assay, and flow cytometry were employed again. KLF5 is highly expressed in OC cells compared with normal cells. When KLF5 knockdowns in SK-OV-3 and OVCAR-3 cells, the cell proliferation restrains, and the G1 phase prolongs. In addition, KLF5 silence caused a decrease of Cyclin D1, CDK4, CDK6, p-p38, p-ERK1/2, and p-ERK5/ERK5 expression levels. However, these statuses could be revised by C16-PAF + EGF. Results also found that when the ERK/MAPK signaling is activating, RREB1 is expressed low. The KLF5 silence could up-regulate the RREB1 expression. The KLF5 silence could restrain the OC cell proliferation and cell cycle. KLF5-siRNA may target upregulating RREB1 expression, thereby inhibiting the activation of the ERK/MAPK signaling pathway in OC cells.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"4"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11635078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile preparation of polyethyleneimine-conjugated silver sulfide nanoparticles as near-infrared-responsive to sterilization of multidrug resistant uropathogens, and cytotoxicity activity. 聚乙烯亚胺偶联硫化银纳米粒子的制备及其对多药耐药尿路病原体的近红外杀菌反应及细胞毒性活性。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-12 DOI: 10.1007/s13205-024-04168-3

Hind A Al-Shwaiman, Rustem R Zairov, Alexey P Dovzhenko, Asad Syed, Manjula Subramaniam, Ling Shing Wong, Baadal Jushi Janani

{"title":"Facile preparation of polyethyleneimine-conjugated silver sulfide nanoparticles as near-infrared-responsive to sterilization of multidrug resistant uropathogens, and cytotoxicity activity.","authors":"Hind A Al-Shwaiman, Rustem R Zairov, Alexey P Dovzhenko, Asad Syed, Manjula Subramaniam, Ling Shing Wong, Baadal Jushi Janani","doi":"10.1007/s13205-024-04168-3","DOIUrl":"10.1007/s13205-024-04168-3","url":null,"abstract":"We present the chemical synthesis of polyethyleneimine-conjugated silver sulfide nanoparticles (PEI/AS) utilizing an economical solvothermal synthesis method, aimed at developing effective alternative antibacterial agents. The antibacterial efficacy of the synthesized materials, both with and without the application of near-infrared (NIR) laser irradiation, was evaluated in vitro against two distinct clinically relevant multi-drug-resistant (MDR) uropathogenic strains: Escherichia coli and methicillin-resistant Staphylococcus aureus. The bactericidal effects induced by NIR light indicate that the PEI/AS nanoparticles possess an efficiency that is five times greater than that of Ag2S alone. A suggested antibacterial mechanism posits that the wrapping of PEI increases electrostatic interactions, thereby facilitating the attachment of Ag2S nanoparticles to the bacterial surface. This process leads to the disruption of the outer membrane through the generation of localized heat and an increased concentration of reactive oxygen species (ROS), including superoxide anions (·O2 -) and hydroxyl radicals (·OH). In addition, the mechanism involves the regulated release of Ag+ ions when exposed to NIR light irradiation. The combined action led to an over 95.79% elimination of bacteria at a concentration as low as 50 μg mL-1, which can be primarily ascribed to the regulated photothermal effect induced by 808 nm near-infrared light irradiation, demonstrating exceptional photothermal conversion efficiency. These results paves a way for manufacturing innovation in future.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04168-3.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"8"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11638448/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic exploration of Surfactin-capped silver nanoparticles: bioinformatics insights, antibacterial potency, and anticancer activity. 表面素覆盖银纳米粒子的协同探索：生物信息学见解，抗菌效力和抗癌活性。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-16 DOI: 10.1007/s13205-024-04174-5

Vivek Chauhan, Akash Pandey, Gaytri Mahajan, Vivek Dhiman, Shamsher S Kanwar

{"title":"Synergistic exploration of Surfactin-capped silver nanoparticles: bioinformatics insights, antibacterial potency, and anticancer activity.","authors":"Vivek Chauhan, Akash Pandey, Gaytri Mahajan, Vivek Dhiman, Shamsher S Kanwar","doi":"10.1007/s13205-024-04174-5","DOIUrl":"10.1007/s13205-024-04174-5","url":null,"abstract":"Surfactin lipopeptides (LPs) are a compelling class of biosurfactants with notable antimicrobial and anticancer properties. This study presents a novel approach by integrating bioinformatics tools to assess the drug potential of Surfactin, specifically focusing on its antibacterial, antifungal activities, and cancer cell-line toxicity. Silver nanoparticles (AgNPs) were synthesized using Surfactin, a biosurfactant derived from Bacillus subtilis KLP2016, as a capping agent, both in the presence and absence of Surfactin, to evaluate its impact on nanoparticle stability and bioactivity. The Surfactin-capped AgNPs demonstrated enhanced stability, uniformity, and antimicrobial efficacy, confirmed through UV-VIS spectroscopy, FE-SEM, and X-ray diffraction analysis. The bioinformatics approach, including ADMET and PASS analysis, revealed the potential of Surfactin as a potent antimicrobial and anticancer agent. In addition, molecular docking studies further validated the interaction of Surfactin with key microbial cell-wall enzymes and proteins, underscoring its therapeutic potential. These findings suggest that Surfactin-stabilized AgNPs, combined with bioinformatic predictions, could pave the way for innovative antimicrobial and anticancer therapies.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"13"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11649612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142852055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PEGylated iron oxide-gold core-shell nanoparticles for tumor-targeted delivery of Rapamycin. 用于肿瘤靶向输送雷帕霉素的 PEG 化氧化铁-金核壳纳米粒子。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-25 DOI: 10.1007/s13205-024-04189-y

Suhana Koothradan, Safia Nayeem, K K Elyas

{"title":"PEGylated iron oxide-gold core-shell nanoparticles for tumor-targeted delivery of Rapamycin.","authors":"Suhana Koothradan, Safia Nayeem, K K Elyas","doi":"10.1007/s13205-024-04189-y","DOIUrl":"10.1007/s13205-024-04189-y","url":null,"abstract":"Rapamycin analogs are approved by the FDA for breast and renal cancer treatment. Hence, the possibility of nanoparticle-mediated delivery of Rapamycin could be examined. In the present study, PEGylated Gold-core shell iron oxide nanoparticles were used for the targeted delivery of Rapamycin, and R-Au-IONPs were formulated. SEM, XRD, and FTIR determined the smooth spherical morphology, and compositional structure, and confirmed the conjugation of Rapamycin onto the NPs. The in vitro drug release study showed a controlled release of the drug over time. R-Au-IONPs showed significant cytotoxicity in MCF 7 cells. Anti-proliferative assays such as trypan blue dye exclusion assay, microscopy, Fluorescent staining, and clonogenic assays were performed. NH staining, Rhodamine 123 staining, PS externalization, and the cleavage of PARP protein by western immunoblot assays confirmed the induction of apoptosis. The mechanism of R-Au-IONP-induced cell death was analyzed by flow cytometry. Our in-vitro study, on the impact of R-Au-IONPs on cell viability in the human breast adenocarcinoma cell line (MCF-7), confirms the efficacy of drug delivery using the nanoparticle system. Further results implied the induction of apoptosis. This drug delivery system using Rapamycin could be a potential candidate in the treatment of breast cancer.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"23"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11669639/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142902345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid detection of cardamom mosaic virus in crude plant extracts using reverse transcription-recombinase polymerase amplification-lateral flow assay (RT-RPA-LFA). 逆转录重组酶-聚合酶扩增-横向流动法（RT-RPA-LFA）快速检测粗植物提取物中的豆蔻花叶病毒。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-30 DOI: 10.1007/s13205-024-04191-4

M Greeshma, A I Bhat

{"title":"Rapid detection of cardamom mosaic virus in crude plant extracts using reverse transcription-recombinase polymerase amplification-lateral flow assay (RT-RPA-LFA).","authors":"M Greeshma, A I Bhat","doi":"10.1007/s13205-024-04191-4","DOIUrl":"10.1007/s13205-024-04191-4","url":null,"abstract":"Cardamom mosaic virus causing mosaic/katte disease is the most destructive virus infecting cardamom. The development of effective diagnostic assays is essential for the production of virus-free plants, as the primary spread of the virus occurs through vegetative propagation. Currently used PCR-based assays are not suitable for Point-of-Care testing, require sophisticated equipment, and are time-consuming. Hence, in the present study, an assay based on reverse transcription-recombinase polymerase amplification (RT-RPA) combined with lateral flow assay (RT-RPA-LFA) was optimized for the specific, and sensitive detection of CdMV. The forward and reverse primers selected for RT-RPA were labeled with 6-carboxyfluorescein (FAM) and biotin respectively at the 5´end. The tedious total RNA preparation was avoided by using the crude extract as a template for the assay. A magnesium acetate concentration of 14 mM, 0.4 M betaine, temperature from 37 to 42 ℃, and 20 min of incubation time were found optimum for the assay. The entire RT-RPA-LFA from sample preparation to visualization of results could be completed within 40-50 min and the assay is suitable for Point-of-Care testing. The assay is specific for CdMV and could detect the virus up to 10-5 dilutions of the crude extract. The assay was validated using field samples collected from different cardamom-growing regions of Kerala and Karnataka, India.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04191-4.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"28"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11685339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Organocatalysed synthesis of N-(4-oxo-2-phenyl-1,2-dihydroquinazolin-3(4H)-yl)isonicotinamide: computational, electrochemical, drug-likeness and antimicrobial studies. 有机催化合成N-（4-氧-2-苯基-1,2-二氢喹唑啉-3(4H)-基）异烟酰胺：计算、电化学、药物相似性和抗菌研究。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2025-01-03 DOI: 10.1007/s13205-024-04188-z

Radhika R Mane, Deepak A Yaraguppi, Zabin K Bagewadi, Kantharaju Kamanna

{"title":"Organocatalysed synthesis of N-(4-oxo-2-phenyl-1,2-dihydroquinazolin-3(4H)-yl)isonicotinamide: computational, electrochemical, drug-likeness and antimicrobial studies.","authors":"Radhika R Mane, Deepak A Yaraguppi, Zabin K Bagewadi, Kantharaju Kamanna","doi":"10.1007/s13205-024-04188-z","DOIUrl":"10.1007/s13205-024-04188-z","url":null,"abstract":"We have developed novel and sustainable homogeneous catalysts employing Glutamic acid (Glu) as a biodegradable and eco-friendly organocatalyst for the synthesis of N-(4-oxo-2-phenyl-1,2-dihydroquinazolin-3(4H)-yl)isonicotinamide derivatives (5a-l) via multicomponent reactions (MCRs) of isatoic anhydride, isoniazid and heteroaromatic/aromatic aldehyde in ethanol on oil bath stirring at 60 °C. Selected final product homogeneity was examined by various spectroscopic techniques such as 13C-, 1H- NMR, FT-IR and LC-MS. For the first time, herein investigated electrochemical behavior of selected derivatives (5c, 5h-l) using cyclic voltammetry method. The results of this investigation indicated compounds 5i, 5h and 5l exhibited highest levels of oxidation and reduction potential. Further, pharmacokinetic properties were assessed via SwissADME online tool, derivatives tested complied with Lipinski's rule of five for drug-likeness. Furthermore, molecular docking studies demonstrated for significant binding between the protein and ligand, and affinity values ranged from - 8.91 to - 8.45 kcal/mol, and MM/PBSA estimated high negative values suggested significant interactions between ligand and protein. Moreover, antibacterial evaluation of compounds 5i in water, and 5k in DMSO on Salmonella typhimurium showed pronounced effect with inhibition zone of 15 ± 0.6 mm and 20 ± 0.4 mm, respectively as compared to the standard tetracycline inhibition zone of 15 ± 0.5 mm.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04188-z.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"30"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699011/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the use of CRISPR/Cas9 gene editing machinery as a cancer therapeutic tool with the help of nanomedicine. 在纳米医学的帮助下，改进CRISPR/Cas9基因编辑机制作为癌症治疗工具的使用。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-19 DOI: 10.1007/s13205-024-04186-1

Hina Fatima, Dimple Singh, Huzaifa Muhammad, Swati Acharya, Mohammad Azhar Aziz

{"title":"Improving the use of CRISPR/Cas9 gene editing machinery as a cancer therapeutic tool with the help of nanomedicine.","authors":"Hina Fatima, Dimple Singh, Huzaifa Muhammad, Swati Acharya, Mohammad Azhar Aziz","doi":"10.1007/s13205-024-04186-1","DOIUrl":"10.1007/s13205-024-04186-1","url":null,"abstract":"CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-associated protein 9) has revolutionized gene editing tools and paved the way for innovations in medical research for disease diagnosis and treatment. However, better specificity and efficient delivery of this gene machinery make it challenging to successfully edit genes for treating various diseases. This is mainly due to cellular barriers, instability in biological environments, and various off-target effects that prohibit safe and efficient delivery under in vivo conditions. This review examines several delivery modes [plasmid, mRNA, RNP (ribonucleoprotein)] and methods for the CRISPR-Cas9 system delivery, focusing on its potential applications in cancer therapy. Biocompatibility and cytotoxicity are crucial factors determining their safe and effective use. Various nanomaterials have been reviewed for their biocompatibility, limitations, and challenges in treating cancer. Among the reviewed nanoparticles, lipid nanoparticles (LNPs) stand out for their biocompatibility due to their biomimetic lipid bilayer that effectively delivers CRISPR/Cas9 cargoes while reducing toxicity. We discuss challenges in in vivo delivery and associated findings such as encapsulation, target delivery, controlled release, and endosomal escape. Future directions involve addressing limitations and adapting CRISPR-Cas9 for clinical trials, ensuring its safe and effective use.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"17"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11656010/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparing the effectiveness of curcumin and papain in wound dresses based on chitosan nanoparticle. 基于壳聚糖纳米颗粒的姜黄素和木瓜蛋白酶在伤口敷料中的效果比较。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-28 DOI: 10.1007/s13205-024-04193-2

Niloufar Elhami, Mohammad Pazhang, Younes Beygi-Khosrowshahi, Amir Dehghani

{"title":"Comparing the effectiveness of curcumin and papain in wound dresses based on chitosan nanoparticle.","authors":"Niloufar Elhami, Mohammad Pazhang, Younes Beygi-Khosrowshahi, Amir Dehghani","doi":"10.1007/s13205-024-04193-2","DOIUrl":"10.1007/s13205-024-04193-2","url":null,"abstract":"In this study, chitosan/curcumin (CS/Cur) and chitosan/papain (CS/Pa) nanoparticles were prepared and then characterized by Fourier transform infrared (FTIR), X-ray diffraction (XRD), and differential light scattering (DLS). Subsequently, release rate, porosity, swelling, degradability, anti-inflammatory, antioxidant, antibacterial, and cell viability tests were conducted to investigate and compare the healing potential of the nanoparticles for various types of wounds. The results of FTIR, XRD, and DLS indicated that the nanoparticles were manufactured correctly with a hydrodynamic diameter of 429 nm (CS/Cur) and 460 nm (CS/Pa), and zeta potential of 4.32 mV (CS/Cur) and 7.57 mV (CS/Pa). The release rate results indicated a higher release rate in a basic environment (pH 8.4) for curcumin, a higher release rate for papain in an acidic environment (pH 6.4), and the Korsmeyer-Peppas model for the release of curcumin and papain. The results indicated that CS/Cur with 41.6% antioxidant activity, high antibacterial effect, and cell growth up to 616% during 7 days, was more effective than CS/Pa. In comparison, CS/Pa (with a porosity of 70.5% and a swelling rate of 1392%) was more advantageous than CS/Cur in terms of porosity and swelling. In addition, CS/Cur was as effective as CS/Pa in terms of degradation and anti-inflammatory properties. In conclusion, the outcomes represented that the CS/Cur and CS/Pa nanoparticles improved wound healing, and each was suitable for specific wounds and wound healing stages.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"27"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

POU4F1 enhances lung cancer gemcitabine resistance by regulating METTL3-dependent TWF1 mRNA N6 adenosine methylation. POU4F1通过调节mettl3依赖的TWF1 mRNA N6腺苷甲基化增强肺癌吉西他滨耐药。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-12 DOI: 10.1007/s13205-024-04161-w

Jianfeng Tang, Zhijian Liu, Guanghui Xie, Chenbin Wang, Yongjun Jiang

{"title":"POU4F1 enhances lung cancer gemcitabine resistance by regulating METTL3-dependent TWF1 mRNA N6 adenosine methylation.","authors":"Jianfeng Tang, Zhijian Liu, Guanghui Xie, Chenbin Wang, Yongjun Jiang","doi":"10.1007/s13205-024-04161-w","DOIUrl":"10.1007/s13205-024-04161-w","url":null,"abstract":"This study aimed to investigate the role of POU Class 4 Homeobox 1 (POU4F1) in regulating gemcitabine (GEM) resistance in lung cancer cells. The mRNA and protein expressions were assessed using RT-qPCR, western blot, immunofluorescence, and immunohistochemistry. Cell viability and proliferation were assessed by CCK-8 assay and EdU assay. TUNEL staining and flow cytometry were employed to detect cell apoptosis. The m6A modification of TWF1 was detected using MeRIP assay. The interactions between molecules were validated using dual luciferase reporter gene, ChIP, and RIP assays. POU4F1 knockdown inhibited GEM resistance and autophagy in lung cancer cells. Mechanistically, POU4F1 transcriptionally activated methyltransferase-like protein 3 (METTL3) in GEM-resistant cells by binding to the METTL3 promoter. METTL3 promoted the N6-methyladenosine (m6A) modification and expression level of twinfilin-1 (TWF1). Overexpression of METTL3 and TWF1 weakened the effects of POU4F1 knockdown on GEM resistance and autophagy. Moreover, knockdown POU4F1 also enhanced GEM anti-tumor sensitivity in vivo. In conclusion, POU4F1 upregulation promoted GEM resistance in lung cancer cells by promoting autophagy through increasing METTL3-mediated TWF1 m6A modification.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04161-w.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"7"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11638459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142827102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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