3 Biotech最新文献_第9页

Groundnut bud necrosis virus encoded movement protein NSm binds to GTP and ATP. 花生芽坏死病毒编码运动蛋白NSm与GTP和ATP结合。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-05-01 Epub Date: 2025-04-30 DOI: 10.1007/s13205-025-04305-6

Rishi Raj, Abhay Kumar, H S Savithri, Pratibha Singh

{"title":"Groundnut bud necrosis virus encoded movement protein NSm binds to GTP and ATP.","authors":"Rishi Raj, Abhay Kumar, H S Savithri, Pratibha Singh","doi":"10.1007/s13205-025-04305-6","DOIUrl":"10.1007/s13205-025-04305-6","url":null,"abstract":"Groundnut bud necrosis virus (GBNV) is a tripartite negative sense RNA virus that belongs to tospoviridae family. The M RNA encodes non-structural protein-m (NSm), a movement protein in tospoviruses. In this communication, we demonstrate that, GBNV NSm interacts with ATP and GTP. UV crosslinking with [γ-32P] ATP indicates that GBNV NSm forms two distinct complexes with ATP one of them is Mg2+ dependent and the other is Mg2+ independent. It also binds to ATP- and GTP-coupled agarose resin and shows competition with free ATP and GTP but not with UTP and CTP. The NSm-NTP interaction was further validated by intrinsic fluorescence quenching studies. NTPs and dNTPs both could quench the intrinsic fluorescence of NSm. However, maximum quenching of fluorescence occurred in the presence of GTP, followed by ATP, suggesting that it is the preferred ligand. The extent of fluorescence quenching with different concentrations of GTP was used to calculate the binding constant, and it was found to be 3 μM, lower than that reported for other proteins that can bind NTP. This is the first report of the GTP and ATP binding property of NSm from any Tospoviruses. Further, NSm could also hydrolyze GTP. Preliminary sequence analysis suggests the presence of two putative atypical Walker A motif from amino acid sequences 51-58 and 267-274, indicating that this sequence might be involved in NTP binding. This motif is conserved in most of the tospoviruses. NSm from GBNV an Asian clade, localize to ER network and remodels it to vesicles which has been proposed to be involved in movement through plasmodesmata (PD). Therefore, GTP-NSm interaction might be involved in signaling cell to cell trafficking.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 5","pages":"146"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044118/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of autophagy and Alzheimer's disease with special emphasis on the role of phosphodiesterase-4. 自噬与阿尔茨海默病的相关性，特别强调磷酸二酯酶-4的作用。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-05-01 Epub Date: 2025-04-23 DOI: 10.1007/s13205-025-04306-5

Aniruddha Banerjee, Dithu Thekkekkara, S N Manjula, Salini P Nair, Mankala Sree Lalitha

{"title":"Correlation of autophagy and Alzheimer's disease with special emphasis on the role of phosphodiesterase-4.","authors":"Aniruddha Banerjee, Dithu Thekkekkara, S N Manjula, Salini P Nair, Mankala Sree Lalitha","doi":"10.1007/s13205-025-04306-5","DOIUrl":"10.1007/s13205-025-04306-5","url":null,"abstract":"Autophagy disruption is important in Alzheimer's disease (AD) as it prevents misfolded proteins from being removed, which leads to the accumulation of amyloid plaques and neurofibrillary tangles (NFTs). Restoring autophagy improves neuronal survival and cognitive function, according to experimental models. In AD models, mTOR inhibition and AMPK activation enhance synaptic plasticity and lessen learning deficits. Inhibitors of phosphodiesterase-4 (PDE4) improve cognition and reduce neuroinflammation via altering cyclic adenosine monophosphate (cAMP) transmission. Furthermore, autophagic-lysosomal clearance is encouraged by upregulating transcription factor EB (TFEB), which lessens the pathogenic damage linked to AD. These results point to autophagy modification as a promising therapeutic approach, with the mTOR, AMPK, cAMP, and TFEB pathways being possible targets for drugs. Though much evidence is based on animal studies, these findings provide valuable insights into autophagy's role in AD pathology, offering promising directions for future research and drug development.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 5","pages":"139"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12018668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143951890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marine polysaccharides from green seaweeds belonging to the class Ulvophyceae mitigate cyclophosphamide-induced immunosuppression: an in vivo study. 绿海藻中的海洋多糖减轻环磷酰胺诱导的免疫抑制：一项体内研究。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-05-01 Epub Date: 2025-04-23 DOI: 10.1007/s13205-025-04307-4

Shilpa Kamalakar Pai, Akkarapaka Neelima, Kajal Chakraborty, Lokanatha Valluru, Shubhajit Dhara

{"title":"Marine polysaccharides from green seaweeds belonging to the class Ulvophyceae mitigate cyclophosphamide-induced immunosuppression: an in vivo study.","authors":"Shilpa Kamalakar Pai, Akkarapaka Neelima, Kajal Chakraborty, Lokanatha Valluru, Shubhajit Dhara","doi":"10.1007/s13205-025-04307-4","DOIUrl":"10.1007/s13205-025-04307-4","url":null,"abstract":"Polysaccharides derived from seaweeds possess notable immunomodulatory and antioxidant properties. This study investigated the therapeutic potential of polysaccharides extracted from four green seaweed species-Ulva reticulata (URP), Ulva lactuca (ULP), Ulva fasciata (UFP), and Caulerpa racemosa (CRP)-in a cyclophosphamide-induced immunosuppressed in vivo model. Polysaccharides were administered at varying doses over 14 days, with 150 mg/Kg body weight (BW) demonstrating the most pronounced effects. ULP treatment significantly restored body weight (199.5 g) compared to the cyclophosphamide group (155.92 g) and notably improved organ weight indices, particularly liver indices (5.28%). Hematological parameters showed marked improvements, including increased red blood cell count (7.55 × 106/μL), hemoglobin levels (10.98 g/dL), and total leukocyte count (13.78 × 103/μL). Immunoglobulin concentrations were significantly elevated, with IgG reaching 1125.16 and IgM 130.3 mg/dL, alongside a substantial enhancement in survival rates (78.3% vs. 22.4% in the cyclophosphamide group). Antioxidant enzyme activities were significantly enhanced, including superoxide dismutase (96.4%), catalase (0.20 U/mg protein), and lactate dehydrogenase (4.11 μg formazan). Among the species tested, ULP exhibited the most potent immunomodulatory and antioxidant effects, mitigating cyclophosphamide-induced immunosuppression and oxidative stress, thereby highlighting its potential as a therapeutic candidate for immune-related disorders.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04307-4.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 5","pages":"140"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12018667/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Osteo-inductive potential of homoeopathy potencies of Asafoetida on multipotent mesenchymal stem cells in vitro. 刺尾草顺势疗法对多能间充质干细胞的体外成骨诱导潜力。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-05-01 Epub Date: 2025-04-12 DOI: 10.1007/s13205-025-04245-1

Sudhansu Sekhar Moharana, Sumit Mallick, Joel Rimson Pinto, Sudheer Shenoy P, Bipasha Bose

{"title":"Osteo-inductive potential of homoeopathy potencies of Asafoetida on multipotent mesenchymal stem cells in vitro.","authors":"Sudhansu Sekhar Moharana, Sumit Mallick, Joel Rimson Pinto, Sudheer Shenoy P, Bipasha Bose","doi":"10.1007/s13205-025-04245-1","DOIUrl":"10.1007/s13205-025-04245-1","url":null,"abstract":"The musculoskeletal system is the main framework of the human system. Poor osteogenesis is related to poor diet, improper exercise, autoimmune diseases, or age-related conditions. This tends to compromise the quality of human life, especially gait and mobility. Calcium, magnesium, and vitamin D supplements are the prescriptions that address the issues above. Moreover, various natural products in alternative medicines have also been found to have applications in improving bone health. One such finding in our study is the homeopathy potency of Asafoetida, 200CH, which has exhibited a promising osteo-inductive potential in vitro for murine multipotent mesenchymal stem cell line C3H10T1/2. Ferula asafoetida is otherwise a known herb for addressing gastric issues. In this work, the homoeopathy clinician (first author) first repurposed the F. asafoetida potency (dilution) to ameliorate bone disorders such as avascular necrosis and loose body dissolution. Following clinical success, we have proven the osteo-inductive properties of this particular homoeopathy potency of the drug in vitro. Most importantly, the osteo-inductive (osteogenesis) properties of homoeopathy potency 200CH are more pronounced than the native compound combinations in the Mother tincture (Q) of the drug. This homoeopathy drug, hence, can be used for treating osteoarthritis alone or else as an adjuvant with conventional treatment.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04245-1.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 5","pages":"124"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11993510/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Omics studies reveal the response mechanisms of Corynebacterium glutamicum to l-homoserine osmotic stress. 组学研究揭示了谷氨酸棒状杆菌对l-同型丝氨酸渗透胁迫的响应机制。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-05-01 Epub Date: 2025-04-16 DOI: 10.1007/s13205-025-04304-7

Jian Wang, Yicun Lin, Jian Yang, Yuxiang Chen, Ning Xu, Jun Liu, Wei Sun, Dawei Li

{"title":"Omics studies reveal the response mechanisms of Corynebacterium glutamicum to l-homoserine osmotic stress.","authors":"Jian Wang, Yicun Lin, Jian Yang, Yuxiang Chen, Ning Xu, Jun Liu, Wei Sun, Dawei Li","doi":"10.1007/s13205-025-04304-7","DOIUrl":"10.1007/s13205-025-04304-7","url":null,"abstract":"To investigate the mechanism of osmotic stress produced by Corynebacterium glutamicum (C. glutamicum) in the production of l-homoserine. The present study employed genomic and transcriptomic analyses of both evolved strains and the parental strain grown under l-homoserine osmotic stress to investigate the response mechanisms and identify specific tolerance targets. The results indicated that the evolved strain enhanced its tolerance to l-homoserine stress by inactivating aspartokinase, thereby interrupting the intracellular synthesis pathway of l-homoserine. Early in stress exposure, C. glutamicum suppressed the synthesis of l -homoserine and instead enhanced its catabolic activity. In response to osmotic stress, C. glutamicum also relied on a variety of energy metabolism and ion transport pathways, including ABC transporters and ATP metabolism, which are essential for high-osmolarity tolerance. Given the gradual accumulation of l-homoserine within the cell, this study focused on the transcriptional expression patterns during the adaptation phase, excluding cellular responses during the high-concentration stress phase. These findings provide valuable insights for improving C. glutamicum's tolerance to l-homoserine stress during amino-acid fermentation and highlight potential targets for metabolic engineering strategies.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04304-7.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 5","pages":"127"},"PeriodicalIF":2.6,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12003223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Conventional and cutting-edge advances in plant virus detection: emerging trends and techniques. 植物病毒检测的传统和前沿进展：新趋势和技术。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-04-01 Epub Date: 2025-03-25 DOI: 10.1007/s13205-025-04253-1

Anjana Singh, Yasheshwar, Naveen K Kaushik, Deepak Kala, Rupak Nagraik, Shagun Gupta, Ankur Kaushal, Yashika Walia, Sunny Dhir, Md Salik Noorani

{"title":"Conventional and cutting-edge advances in plant virus detection: emerging trends and techniques.","authors":"Anjana Singh, Yasheshwar, Naveen K Kaushik, Deepak Kala, Rupak Nagraik, Shagun Gupta, Ankur Kaushal, Yashika Walia, Sunny Dhir, Md Salik Noorani","doi":"10.1007/s13205-025-04253-1","DOIUrl":"10.1007/s13205-025-04253-1","url":null,"abstract":"Plant viruses pose a significant threat to global agriculture. For a long time, conventional methods including detection based on visual symptoms, host range investigations, electron microscopy, serological assays (e.g., ELISA, Western blotting), and nucleic acid-based techniques (PCR, RT-PCR) have been used for virus identification. With increased sensitivity, speed, and specificity, new technologies like loop-mediated isothermal amplification (LAMP), high-throughput sequencing (HTS), nanotechnology-based biosensors, and CRISPR diagnostics have completely changed the way plant viruses are detected. Recent advances in detection techniques integrate artificial intelligence (AI), machine learning (ML), and the Internet of Things (IoT) for real-time monitoring. Innovations like hyperspectral imaging, deep learning, and cloud-based IoT platforms further support disease identification and surveillance. Nanotechnology-based lateral flow assays and CRISPR-Cas systems provide rapid, field-deployable solutions. Despite these advancements, challenges such as sequence limitations, multiplexing constraints, and environmental concerns remain. Future research should focus on refining portable on-site diagnostic kits, optimizing nanotechnology applications, and enhancing global surveillance systems. Interdisciplinary collaboration across molecular biology, bioinformatics, and engineering is essential to developing scalable, cost-effective solutions for plant virus detection, ensuring agricultural sustainability and ecosystem protection.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"100"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unlocking the potential of ecofriendly guardians for biological control of plant diseases, crop protection and production in sustainable agriculture. 释放生态友好型监护人在植物病害生物防治、作物保护和可持续农业生产方面的潜力。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-04-01 Epub Date: 2025-03-09 DOI: 10.1007/s13205-025-04243-3

Diksha Malik, Satish Kumar, Satyavir S Sindhu

{"title":"Unlocking the potential of ecofriendly guardians for biological control of plant diseases, crop protection and production in sustainable agriculture.","authors":"Diksha Malik, Satish Kumar, Satyavir S Sindhu","doi":"10.1007/s13205-025-04243-3","DOIUrl":"10.1007/s13205-025-04243-3","url":null,"abstract":"Several beneficial microbial strains inhibit the growth of different phytopathogens and commercialized worldwide as biocontrol agents (BCAs) for plant disease management. These BCAs employ different strategies for growth inhibition of pathogens, which includes production of antibiotics, siderophores, lytic enzymes, bacteriocins, hydrogen cyanide, volatile organic compounds, biosurfactants and induction of systemic resistance. The efficacy of antagonistic strains could be further improved through genetic engineering for better disease suppression in sustainable farming practices. Some antagonistic microbial strains also possess plant-growth-promoting activities and their inoculation improved plant growth in addition to disease suppression. This review discusses the characterization of antagonistic microbes and their antimicrobial metabolites, and the application of these BCAs for disease control. The present review also provides a comprehensive summary of the genetic organization and regulation of the biosynthesis of different antimicrobial metabolites in antagonistic strains. Use of molecular engineering to improve production of metabolites in BCAs and their efficacy in disease control is also discussed. The application of these biopesticides will reduce use of conventional pesticides in disease control and help in achieving sustainable and eco-friendly agricultural systems.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"82"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891127/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of arbuscular mycorrhizal fungi on morpho-biochemical characteristics, nutrient uptake, and transcriptomic profile of Solanum melongena L. plant. 丛枝菌根真菌对茄类植物形态生化特征、营养吸收和转录组学的影响

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-04-01 Epub Date: 2025-03-10 DOI: 10.1007/s13205-025-04247-z

Subhesh Saurabh Jha, L S Songachan

{"title":"Influence of arbuscular mycorrhizal fungi on morpho-biochemical characteristics, nutrient uptake, and transcriptomic profile of Solanum melongena L. plant.","authors":"Subhesh Saurabh Jha, L S Songachan","doi":"10.1007/s13205-025-04247-z","DOIUrl":"10.1007/s13205-025-04247-z","url":null,"abstract":"The present study was carried out to observe the interaction between Solanum melongena L. with AMF treatments at morphological, biochemical, and molecular levels. The S. melongena plant was inoculated with Funneliformis mosseae, Glomus macrocarpum, Glomus monosporum, Acaulospora koskei, Acaulospora mellea and Rhizophagus irregularis, and also two consortia of F. mosseae + G. monosporum and F. mosseae + G. macrocarpum. Morphological and biochemical parameters such as shoot height, root length, shoot fresh weight, shoot dry weight, fruit fresh weight, fruit dry weight, root fresh weigh, root dry weight, chlorophyll a and b, total soluble sugars, total soluble proteins were examined in this study. Uptake of some essential micronutrients was also analyzed in this study. In transcriptomics experiment, a total of 365 transcripts were commonly upregulated (≥ 1.5x) folds in S. melongena in response to both the AMF isolates, while 44 transcripts were upregulated only in response to G. Macrocarpum and 28 transcripts were upregulated only in response to F. mosseae. KEGG pathway analysis of S. melongena treated with G. macrocarpum revealed carbon metabolism, cofactor biosynthesis and endocytosis as the dominant metabolic pathway, while analysis of F. mosseae treatment revealed glycerophospholipid and endocytosis metabolism as dominant metabolic pathways.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04247-z.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"84"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893938/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neuroprotective and antioxidant effects of Urtica dioica extract against chlorpyrifos-induced toxicity: an in vivo study. 荨麻疹提取物抗毒死蜱毒性的神经保护和抗氧化作用：体内研究。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-04-01 Epub Date: 2025-03-12 DOI: 10.1007/s13205-025-04239-z

Ahlem Chira, Imen Dridi, Fatma Rahmouni, Ichrak Ben Amor, Bochra Gargouri, Choumous Kallel, Kamel Jamoussi, Abdelfattah El Feki, Mongi Saoudi

{"title":"Neuroprotective and antioxidant effects of Urtica dioica extract against chlorpyrifos-induced toxicity: an in vivo study.","authors":"Ahlem Chira, Imen Dridi, Fatma Rahmouni, Ichrak Ben Amor, Bochra Gargouri, Choumous Kallel, Kamel Jamoussi, Abdelfattah El Feki, Mongi Saoudi","doi":"10.1007/s13205-025-04239-z","DOIUrl":"10.1007/s13205-025-04239-z","url":null,"abstract":"This study examines the neuroprotective and antioxidant effects of Urtica dioica ethanolic extract (UDExt) and melatonin (Mel) in mitigating chlorpyrifos (CPF)-induced toxicity in rats, with melatonin serving as a standard antioxidant. Male Wistar rats were divided into six groups: (I) Control, (II) UDExt (200 mg/kg), (III) Mel (20 mg/kg), (IV) CPF (10 mg/kg), (V) UDExt + CPF, and (VI) Mel + CPF. UDExt and Mel were administered for 7 days prior to CPF exposure, followed by a treatment of 14 days. The findings revealed that CPF exposure significantly impaired hematological parameters (WBC, PLT, LYM), increased lipid peroxidation and protein oxidation, and decreased antioxidant enzyme activities (SOD, CAT, GPx) in brain and cerebellar tissues compared to control rats. Behavioral tests, including the open field test, showed that CPF reduced locomotor activity and increased anxiogenic symptoms. The Y-maze test revealed impaired recognition memory, and the tail suspension test indicated increased depressive symptoms. Histological analysis revealed neuronal damage in the brain and cerebellum tissues. Treatment with UDExt or melatonin, following CPF exposure, significantly improved hematological parameters, reduced lipid peroxidation and protein oxidation, normalized antioxidant enzyme activities, and alleviated neurobehavioral alterations. Histopathological analysis also showed that UDExt or melatonin combined with CPF notably reduced neuronal damage in the brain and cerebellum. UDExt and melatonin mitigated the CPF-induced neurobehavioral alterations associated oxidative injury.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"86"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11904075/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integration of physio-biochemical, biological and molecular approaches to improve heavy metal tolerance in plants. 整合生理生化、生物学和分子方法提高植物对重金属的耐受性。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-04-01 Epub Date: 2025-03-06 DOI: 10.1007/s13205-025-04248-y

Swathi Shivappa, K P Amritha, Siddharth Nayak, Harsha K Chandrashekar, Sachin Ashok Thorat, Arya Kaniyassery, Nisha Govender, Muthu Thiruvengadam, Annamalai Muthusamy

{"title":"Integration of physio-biochemical, biological and molecular approaches to improve heavy metal tolerance in plants.","authors":"Swathi Shivappa, K P Amritha, Siddharth Nayak, Harsha K Chandrashekar, Sachin Ashok Thorat, Arya Kaniyassery, Nisha Govender, Muthu Thiruvengadam, Annamalai Muthusamy","doi":"10.1007/s13205-025-04248-y","DOIUrl":"10.1007/s13205-025-04248-y","url":null,"abstract":"Heavy metal toxicity hinders plant growth and development by inducing oxidative stress, decreasing biomass, impairing photosynthesis, and potentially leading to plant death. The inherent defense mechanisms employed by plants, including metal sequestration into vacuoles, phytochelation, cell wall metal adsorption and an enhanced antioxidant system can be improved via various approaches to mitigate heavy metal toxicity. This review primarily outlines plants direct and indirect responses to HM stress and the tolerance mechanisms by which plants combat the toxic effects of metals and metalloids to understand the effective management of HMs and metalloids in the soil system. Furthermore, this review highlights measures to mitigate metal and metalloid toxicity and improve metal tolerance through various physio-biochemical, biological, and molecular approaches. This review also provides a comprehensive account of all the mitigative approaches by comparing physio-biochemical, biological and molecular approaches. Finally, we compared all the mitigative approaches used in monocotyledonous and dicotyledonous to increase their metal tolerance. Although many studies have compared monocot and dicot plants based on metal toxicity and tolerance effects, comparisons of these mitigative approaches have not been explored.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"76"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11885775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0