Ability to use ITS and rbcL sequencing for determination of the genetic diversity and relationships among olive (Olea europaea L.) genotypes.

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-06-01 Epub Date: 2025-05-12 DOI:10.1007/s13205-025-04336-z

Sobhan Firouzi, Mahmood Solouki, Bahman Fazeli-Nasab, Ali Salehi-Sardoei, Mehrnaz Hatami, Mansour Ghorbanpour, Baohong Zhang

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引用次数: 0

Abstract

Olives are of considerable economic and commercial importance and are mostly used in both daily life and industries. The DNA barcode method has a lot of potential for reviving the science of arithmetics and traditional biodiversity studies, so it has been widely used on plants and for classification and arithmetic purposes. In this study, we sequenced seven different olive genotypes (Olea europaea cv. Olive yellow, O. europaea cv. Oliy, O. europaea cv. Roodbar Oily, O. europaea cv. Mari, O. europaea cv. Fishemi, O. europaea cv. Manzanila, O. europaea cv. Koroneiki) to study their diversity and evolution. The data were analyzed using Clustalw2 and BioEdit software. The homology rate of rbcL and ITS sequences was all in the range of 97-100%. It was identified, for ITS, 1,059 genetic luci (580 luci without deletion and addition and 479 luci with deletion and addition (328 luci polymorphs, 151 monomorphs), 217 singletons, and for rbcL, 565 genetic luci with ( 60 luci without deletion and addition and 505 luci with deletion and addition (2 luci with polymorphs, 503 monomorphs), 1 singleton. It was determined that the number of four haplotypes (haplotype diversity index = 0.80) was determined for ITS and three haplotypes (haplotype diversity index = 0.71) for RBCL. The results indicated that in the nucleotide sequence of the ITS gene among olive varieties, guanine was the most abundant base at 28.7%, while adenine had the lowest abundance at 5%. In contrast, the rbcL gene showed that thymine was the most abundant base at 29.8%, with cytosine being the least abundant at 20.6%. Estimates of nucleotide transitions in the ITS gene revealed a high frequency of pyrimidine transitions, with a thymine-to-cytosine transition rate of 16.84% and a cytosine-to-thymine transition rate of 11.63%. The ITS primer successfully identified and separated only six genotypes, whereas rbcL identified all seven genotypes. Although the success rates of 60-70% for both ITS and rbcL may not seem particularly high, they still significantly contribute to large-scale biodiversity inventories, especially for olive species.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04336-z.

查看原文本刊更多论文

利用ITS和rbcL测序测定橄榄（Olea europaea L.）基因型之间的遗传多样性和关系。

橄榄具有相当大的经济和商业重要性，主要用于日常生活和工业。DNA条形码方法在恢复算术科学和传统生物多样性研究方面具有很大的潜力，因此它已被广泛应用于植物分类和算术目的。在这项研究中，我们测序了7个不同的橄榄基因型(Olea europaea cv。橄榄黄，欧属植物。欧。欧。奥利。油质木条，欧氏木条属。Mari， O. europaia cv。菲什米，欧。Manzanila， O. europaia cv。Koroneiki)来研究它们的多样性和进化。采用Clustalw2和BioEdit软件对数据进行分析。rbcL与ITS序列的同源率均在97 ~ 100%之间。在ITS中鉴定出1059个遗传luci(580个无缺失和添加的luci， 479个缺失和添加的luci（328个多态，151个单态），217个单态；在rbcL中鉴定出565个无缺失和添加的luci(60个无缺失和添加的luci， 505个缺失和添加的luci（2个多态，503个单态），1个单态。结果表明，ITS有4个单倍型（单倍型多样性指数= 0.80），RBCL有3个单倍型（单倍型多样性指数= 0.71）。结果表明，在橄榄树品种ITS基因的核苷酸序列中，鸟嘌呤的丰度最高，为28.7%，腺嘌呤的丰度最低，为5%。相比之下，rbcL基因显示胸腺嘧啶是最丰富的碱基（29.8%），胞嘧啶是最不丰富的碱基（20.6%）。ITS基因的核苷酸转换估计显示嘧啶转换的频率很高，胸腺嘧啶到胞嘧啶的转换率为16.84%，胞嘧啶到胸腺嘧啶的转换率为11.63%。ITS引物仅成功地鉴定和分离了6个基因型，而rbcL则鉴定了所有7个基因型。尽管ITS和rbcL的60-70%的成功率似乎不是特别高，但它们仍然对大规模生物多样性清单做出了重大贡献，特别是对橄榄物种。补充信息：在线版本包含补充资料，提供地址为10.1007/s13205-025-04336-z。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.