Unlocking the potential of Artemisia annua for artemisinin production: current insights and emerging strategies.

Malaria is a deadly disease, and the best effective treatments depend on artemisinin, a sesquiterpene lactone compound isolated from the plant Artemisia annua. However, artemisinin is produced in very small amount within the plant which is insufficient to meet the global demand. Although researchers have investigated synthetic and semi-synthetic approaches, they still face significant challenges, such as high costs and low efficiency, making A. annua the most viable source. Biotechnological advances in breeding and genetic engineering have developed new A. annua varieties with higher artemisinin content, and some varieties have achieved up to 3.2% of plant dry weight. Furthermore, researchers have identified the key genes and transcription factors that can be modified to boost production further. Environmental factors, such as light and specific plant hormones, play a crucial role in regulating this pathway. Also, tissue culture, hairy root systems, and natural elicitors have shown promising results, but need further refinement. Interestingly, the use of whole plants (such as dried leaf powder) instead of purified artemisinin alone has been found to improve drug absorption in the body, improve its effectiveness, and help combat artemisinin resistance. Beyond treating malaria, A. annua also demonstrates other therapeutic potential in treating other diseases, including cancer and viral infections. These findings highlight that A. annua is not just a source of artemisinin; it is a valuable medicinal plant that deserves continued research focus, primarily through approaches that improve artemisinin production directly in the plant.