3 Biotech最新文献

{"title":"Evaluating the antioxidant-mediated neuroprotection of hamamelitannin against H₂O₂-induced oxidative damage.","authors":"Vishnu Adith Janarthanam, Rupesh Gupta, Alaa S Alhegaili, Ajay Guru, Praveen Kumar Issac","doi":"10.1007/s13205-025-04379-2","DOIUrl":"10.1007/s13205-025-04379-2","url":null,"abstract":"<p><p>Excessive generation of free radicals disrupts redox equilibrium, inducing oxidative stress. Dementia and other related neurodegenerative illnesses are important ailments associated with oxidative stress. Antioxidant treatment proves effective in reducing oxidative stress and associated neurodegeneration. This study examined the therapeutic potential of Hamamelitannin, a hydrolyzable tannin derived from the barks of <i>Hamamelis virginiana</i> L., against the hydrogen peroxide-induced oxidative stress. Hamamelitannin (10-50 μM) had no effects on the hatching, heart rates, and also no lethal malformations were observed. Hammamelitannin exposure significantly (p < 0.05) improved the alterations in swimming behavior and the cognitive impairment caused by H₂O₂ in a concentration-dependent manner from 10 to 50 μM. It also showed significant restoration of antioxidant enzymes (SOD, CAT, GPx, GST, and GSH), and acetylcholinesterase activity was observed in larvae exposed to hydrogen peroxide compared to the control. Hamamelitannin lowered the MDA and nitric oxide levels in group treated with H₂O₂. The Cell death and intracellular ROS levels were assessed using acridine orange and dichlorofluorescein diacetate staining methods. Hamamelitannin significantly (p < 0.05) reduced apoptosis and ROS levels caused by hydrogen peroxide. Furthermore, Hamamelitannin upregulated the expression of antioxidant enzyme genes, indicating its potential in combating oxidative stress. These findings suggest that Hamamelitannin has substantial therapeutic value against neurological disorders associated with ROS accumulation.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 7","pages":"224"},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12183148/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144482855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification, optimisation and production of xylooligosaccharides by heat-tolerant xylanase-producing strain.","authors":"Xueyu Qiu, Qijie Sun, Chunyan Huo, Weiwei Fu, Ning Cai, Peng Gao, Chao Teng","doi":"10.1007/s13205-025-04394-3","DOIUrl":"10.1007/s13205-025-04394-3","url":null,"abstract":"<p><p>A novel heat-resistant xylanase-producing strain (<i>Thermomyces dupontii</i> J22) isolated from a soil sample in China can produce a high level of cellulase-free xylanase (768.32 U/mL). The conditions for xylanase production by liquid fermentation were optimised through the implementation of single factor experiments and a response surface experiment. The highest xylanase production was obtained when corn cob was added at a 5% concentration as carbon source, yeast paste, tryptone and ammonium dihydrogen phosphate (3:1:1) were added at a total concentration of 3% as nitrogen source, the initial pH was 7.2 and the temperature was 47.5 ℃. The crude enzyme exhibited the highest activity for xylanase at 80 degrees Celsius and pH 6. The crude enzyme was employed in the enzymatic production of xylooligosaccharides (XOS) from hydrothermal extracts of corn cobs, with the optimum reaction temperature and pH identified as 60 ℃ and 7.0, respectively. The final XOS is primarily constituted of xylobiose and xylotriose, representing up to 74.29% of the total composition.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 7","pages":"228"},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12205120/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Riding the wave of innovation: nanotechnology in nucleic acid-based cancer therapy.","authors":"Kavita, Bhupinder Singh, Teenu Sharma","doi":"10.1007/s13205-025-04397-0","DOIUrl":"10.1007/s13205-025-04397-0","url":null,"abstract":"<p><p>Nucleic acids have lately been explored as one of the significant strategies for cancer treatment. Despite their potential as a promising therapeutic tool in cancer therapy, these often face challenges of rapid degradation, insufficient cellular uptake, and poor targeting abilities. In this regard, nanotechnology has revolutionized nucleic acid-based cancer therapy. The current review explores role of various delivery carriers such as lipidic nanoparticles, polymeric nanoparticles, metallic nanoparticles, and quantum dots in facilitating the transport of nucleic acids, viz., plasmid DNA, siRNA, mRNA, and aptamers. Nanocarriers tend to protect nucleic acids from degradation, improve cellular internalization, and deliver nucleic acids to specific cellular regions, thereby improving their efficacy. In addition, this review provides a comprehensive overview of targeting strategies that address multiple biological barriers of systemic circulation to intracellular trafficking of nucleic acids, as well as advantages, limitations, and applications of each nanoparticles type. By analyzing the current state of scientific research and future advancements in clinical innovation, this review highlights the transformative potential and practical challenges to nanotechnology in nucleic acid-based cancer therapy.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 7","pages":"226"},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12204975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-infective synergy of <i>Acacia nilotica</i> bioactive fraction and methyl gallate with meropenem against pathogenicity of <i>Pseudomonas aeruginosa</i>.","authors":"Samreen, Iqbal Ahmad","doi":"10.1007/s13205-025-04372-9","DOIUrl":"10.1007/s13205-025-04372-9","url":null,"abstract":"<p><p>A combination approach using phytocompounds with antibiotics offers a promising strategy to enhance antibiotic efficacy and reduce pathogen virulence. This study investigates the synergistic antibacterial and anti-virulence potential of <i>Acacia nilotica</i> ethyl acetate fraction (ANEF) and its bioactive compound, methyl gallate (MG), in combination with meropenem against <i>Pseudomonas aeruginosa</i> PAO1. The synergy was assessed using checkerboard assays against <i>P. aeruginosa</i> and <i>Escherichia coli</i>, with ANEF-meropenem and MG-meropenem combinations yielding fractional inhibitory concentration index (FICI) values of 0.187 and 0.312, respectively, indicating strong synergism. These combinations significantly reduced bacterial viability, with log₁₀ CFU/ml reductions of 4.48 (ANEF-meropenem) and 3.68 (MG-meropenem). The combinations inhibited quorum sensing (QS)-regulated virulence factors, including pyocyanin, pyoverdine, protease, swarming, and EPS production by 58-82% at ½ FIC. The biofilm disruption assays further confirmed the anti-biofilm potential, with reductions of 86.67% by ANEF-meropenem and 82.66% for the MG-meropenem combination. The mechanistic evaluations revealed enhanced bacterial membrane disruption, with MG-meropenem showing the highest membrane permeability (54.63% dye uptake) and cellular leakage (OD 1.28). Hemolysis assays indicated dose-dependent toxicity, with maximum hemolysis by 45.39% (ANEF) and 29.94% (MG) at the MIC value. However, ANEF exhibited no adverse developmental effects in <i>Caenorhabditis elegans</i> N2 worms. These findings demonstrate that ANEF and MG, in synergy with meropenem, effectively impair <i>P. aeruginosa</i> growth, virulence, and biofilm formation while maintaining low toxicity profiles. The findings support the potential of phytochemical-antibiotic combinations as promising adjunct therapies for multidrug-resistant <i>P. aeruginosa</i> infections.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04372-9.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 7","pages":"209"},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12158871/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted delivery of seaweed bioactives: liposomal encapsulation of <i>Ulva lactuca</i> and <i>Codium fragile</i> for antibacterial enhancement.","authors":"Esra Ak, Pelin Guven, İnci Tuney, Munevver Muge Cagal","doi":"10.1007/s13205-025-04374-7","DOIUrl":"10.1007/s13205-025-04374-7","url":null,"abstract":"<p><p>Seaweeds are promising natural sources of antimicrobials and antioxidants; however, their direct use is often limited by the lack of effective targeted delivery systems. The main objective of this study was to investigate the effects of liposomal encapsulation on enhancing the antimicrobial action of seaweed bioactives. A liposomal formulation was developed to encapsulate extracts from the green seaweeds <i>Ulva lactuca</i> and <i>Codium fragile</i> subsp. <i>fragile</i>, sourced from the Aegean Sea and the Marmara Sea. Physicochemical characterizations of liposomal formulations (size, charge, polydispersity index) were evaluated by dynamic light scattering technique. The encapsulation efficiency (EE%) of liposomal <i>U. lactuca</i> Marmara (Lipo-ULM), liposomal <i>U. lactuca</i> Aegean (Lipo-ULA), liposomal <i>C. fragile</i> subsp<i>. fragile</i> Marmara (Lipo-CM), liposomal <i>C. fragile</i> subsp<i>. fragile</i> Aegean (Lipo-CA) were calculated as 61.63, 70.73, 67.56, and 76.92, respectively. FTIR analysis has confirmed the encapsulation. Antibacterial activities of the free extracts and the liposomal formulations were evaluated against <i>Escherichia coli</i> ATCC 25922 and <i>Staphylococcus aureus</i> ATCC 25923 using minimum inhibitory concentration (MIC) assays. Lipo-ULM and Lipo-ULA displayed MIC values of 1.25 µg/µL against both bacterial strains, compared to 2.5 µg/µL for their free extracts. Lipo-CM and Lipo-CA showed even more activity, with MIC values of 0.0025 µg/µL, while the free extracts of CM and CA presented 0.0050 µg/µL. Furthermore, free and liposomal encapsulated extracts revealed similar DPPH radical scavenging capacity. This study presents a novel liposomal encapsulation system that holds promise for enhancing the effectiveness of edible seaweed extracts as antibacterial agents for application in the pharmaceutical and food industries.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 7","pages":"217"},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12176709/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-dependent apoptotic effect of <i>Woodfordia fruticosa</i> on hepatocellular carcinoma (HepG2) cells by upregulating bax and caspase-9.","authors":"Byeong-Su Kang, Kyubae Lee, Hyeon-Ju Lee, Hyeon-Ji Han, Gyunam Kim, Dain Kim, Eon-Bee Lee","doi":"10.1007/s13205-025-04392-5","DOIUrl":"10.1007/s13205-025-04392-5","url":null,"abstract":"<p><p>This study investigated the dose-dependent apoptotic effect of <i>Woodfordia fruticosa</i> (WF) in HepG2 hepatocellular carcinoma cells, focusing on Bax and caspase-9 upregulation. The ethanol extracts, WF70 (70% ethanol) and WF30 (30% ethanol), were tested for cytotoxicity, pro-apoptotic properties, and cell cycle effects. MTT assays revealed a dose- and time-dependent reduction in cell viability, with IC50 values of 14.39 µg/mL and 15.96 µg/mL for WF70 and WF30, respectively. Flow cytometry analysis showed that WF70 induced significantly higher apoptosis rates (54.82% at 30 µg/mL) than WF30 (27.84%), confirming its stronger pro-apoptotic potential. Cell cycle analysis demonstrated a dose-dependent increase in G₂/M phase arrest, reaching 30.69% and 24.53% with 30 µg/mL WF70 and WF30, respectively. Gene expression analysis revealed a significant upregulation of pro-apoptotic markers (Bax: 3.65-fold, Caspase-9: 4.32-fold, Caspase-3: 2.12-fold) and a marked downregulation of an anti-apoptotic marker (Bcl-2: 0.08-fold) after treatment with 30 µg/mL WF70. Thus, WF70 exerts a stronger apoptotic effect through both intrinsic (mitochondrial) and extrinsic apoptotic pathways, primarily driven by Bax-mediated mitochondrial membrane destabilization and Caspase-9 activation. This study highlights the potential of WF as an anticancer agent for hepatocellular carcinoma, although this warrants further in vivo validation and optimization for therapeutic applications.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 7","pages":"220"},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In silico identification of PPARγ agonists from diffractaic acid analogs in prostate cancer: a comprehensive computational approach.","authors":"Miah Roney, Md Nazim Uddin, Azmat Ali Khan, Mohd Fadhlizil Fasihi Mohd Aluwi, Sabiha Fatima, Asrar Ahmad","doi":"10.1007/s13205-025-04376-5","DOIUrl":"10.1007/s13205-025-04376-5","url":null,"abstract":"<p><p>Prostate cancer (PCa) is the second most frequent and the fifth greatest cause of death in men. Although there are already therapies for early-stage PCa, their effectiveness in advanced PCa is limited, primarily because of medication resistance or poor efficacy. To find new therapeutic indications or repurpose current medications, this project intends to use computational approaches to investigate possible anti-PCa compounds based on simulated screening of FDA-approved drug bank databases. The techniques used in this study include virtual screening of the drug bank database utilising a matrix or user molecule (Diffractaic acid; DA), integrated network pharmacology, molecular docking, detailed molecular dynamic simulation. The results showed that 18 DA analogues in total were chosen from the drug bank database and put through integrated network pharmacology. The KEGG enrichment analysis indicated that hsa05215: Prostate cancer is one of the most significant PCa enrichment signalling pathways which exhibited 18 protein-protein interactions including PDGFRB, PDGFRA, PPARG, GSK3B, MAP2K1, CREBBP, HSP90AA1, HSP90AB1, CHUK, PIK3CD, BRAF, PIK3CB, MTOR, AR, PIK3CA, PLAU, CDK2, and MAPK1. Subsequent molecular docking study with the best target protein (PPARγ) showed that the DB14929 molecule formed hydrogen bonds with the Gln273, Arg280, Arg288, and Ser342 residues and exhibited a high binding affinity ( - 10.5 kcal/mol) for the PPARγ agonist against PCa. Furthermore, molecular dynamic simulation showed that DB14929 formed a stable protein-ligand complex with RMSD, RMSF, Rg, and SASA values. Furthermore, the dynamic behaviour of the PPARγ protein linked to DB14929 in its conformational space was analysed using the PCA technique, demonstrating the excellent conformational space behaviour. Additionally, the free binding energy value of - 57.15 kcal/mol of DB14929 indicated that it could be an agonist of PPARγ of PCa.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04376-5.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 7","pages":"219"},"PeriodicalIF":2.6,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12181459/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the therapeutic efficacy of Bai-Shao in mitigating comorbid epileptic seizures and cognitive impairment via inflammatory signaling pathways: insights from in silico and in vivo studies.","authors":"Yanhong Yu, Hailing Lv, Xiaoyu Liu, Aiju Liu","doi":"10.1007/s13205-025-04316-3","DOIUrl":"10.1007/s13205-025-04316-3","url":null,"abstract":"<p><p>Bai-Shao (BS) ingredients capable of crossing the blood-brain barrier (BBB) were identified and analyzed for their main gene targets. Genes associated with epileptic seizures (ES), cognitive impairment (CI), and inflammation were retrieved, with common targets between BS and these conditions determined via Venn analysis. Protein-protein interaction (PPI) analysis identified the top 10 key genes, whose correlations with apolipoprotein E (APOE) and methylenetetrahydrofolate reductase (MTHFR) were assessed. Functional pathways were explored using Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG), while molecular docking evaluated interactions between BS compounds and target proteins. Findings were validated in a pentylenetetrazol (PTZ)-induced mouse model. BS contains β-sitosterol and betulinic acid, both BBB-permeable, with 277 shared ES/CI-related targets identified. The top 10 genes correlated with APOE and MTHFR, and pathway analysis highlighted interleukin-17 (IL-17) and tumor necrosis factor (TNF) signaling, involving FOS proto-oncogene (FOS), jun proto-oncogene (JUN), caspase-3 (CASP3), phosphoinositide 3-kinase (PI3K), protein kinase B (AKT), and cyclooxygenase-2 (COX2). Behavioral tests, including the Open Field Test and Morris Water Maze, indicated cognitive and motor improvements in PTZ-treated mice. Molecular analysis revealed hippocampal regulation of key genes and altered TNF-alpha (TNFα), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β) levels. In conclusion, BS demonstrates therapeutic potential for comorbid ES and CI by modulating inflammatory pathways and cell survival mechanisms, supporting its role in neurological research.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04316-3.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 6","pages":"171"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12081812/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic activity and pathway study of emerging contaminants biodegradation using a photo-bioelectrochemical system: a review.","authors":"Zohreh Moghiseh, Farnaz Almasi","doi":"10.1007/s13205-025-04340-3","DOIUrl":"10.1007/s13205-025-04340-3","url":null,"abstract":"<p><p>Emerging contaminants and their intermediate residue pose significant risks to human health and ecosystems due to their persistence in water and soil. The review explores the potential of photo-bioelectrochemical systems as a dual-function approach for the degradation of emerging contaminants while simultaneously generating bioenergy. Photo-bioelectrochemical systems integrate microbial metabolism with photocatalytic processes, utilizing solar energy to enhance electron transfer and promote the breakdown of toxic compounds. Recently, this technology has demonstrated improved removal rates of various emerging contaminants, including antibiotics and neonicotinoid pesticides, while producing valuable by-products such as hydrogen and methane through optimized operational conditions. Although studies have focused mainly on pharmaceuticals and dyes, applying these systems for pesticides remains underexplored. Algae-assisted photo-bioelectrochemical systems have been observed frequently in recent studies. This review synthesizes current research on the metabolic activity and biochemical pathways involved in the biodegradation of contaminants within photo-bioelectrochemical systems. It highlights the efficiency of these systems in converting harmful substances into less toxic by-products. It addresses challenges such as slow degradation rates compared to chemical methods and the need for enhanced extracellular electron transfer capabilities among microorganisms. Through optimized operational conditions, this paper aims to provide a comprehensive understanding of photo-bioelectrochemical systems' performance and to propose future research directions for optimizing these systems in real-world applications. In conclusion, this work underscores the promise of photo-bioelectrochemical systems as a sustainable solution for wastewater treatment and environmental remediation.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 6","pages":"173"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12084208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144092585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In-silico</i> molecular investigation of <i>Nannochloropsis</i> microalgae cellulose synthase under salinity conditions and <i>in-vitro</i> evaluation of the proportionate effects on cellulose production.","authors":"Seyed Mohammad Hasan Haghayeghi, Maryam Azimzadeh Irani, Hossein Askari, Madihe Sadat Rasa, Zeinab Shariatmadari","doi":"10.1007/s13205-025-04329-y","DOIUrl":"10.1007/s13205-025-04329-y","url":null,"abstract":"<p><p><i>Nannochloropsis</i> is a microalgae with more than substantially 60-70% cellulose in its cell wall, making it a potential candidate for nanocellulose sustainable production. This study examined the effects of salts in seawater and their role on <i>Nannochloropsis gaditana and Nannochloropsis oculata</i> cellulose synthase activity using <i>In-silico</i> and <i>In-vitro</i> approaches for the first time. Deep-learning-based AlphaFold2 predicted model was selected as the most reliable 3D structure. Molecular docking results revealed that none of the selected ligands occupied the binding site predicted for the native substrate of the enzyme, uridine-diphosphate. To validate the <i>In-silico</i> results, experiments were conducted to investigate the impact of salinity stress (NaCl, NaNO₃ and NaHCO₃) on the cell growth and cellulose production. The assessment tools included a UV-visible spectrophotometer and a hemocytometer, with a modified Jayme-Wise method used for cellulose extraction. The results indicated that the following concentrations of 0.443 mol/L, 0.457 mol/L, and 0.469 mol/L of NaCl, 0.072 mol/L, 0.077 mol/L, and 0.082 mol/L of NaNO3, 0.0021 mol/L, 0.0022 mol/L, and 0.0023 mol/L of NaHCO₃ did not lower the growth rate nor the cellulose yield of <i>N. oculata</i> and notable enhancement in growth was observed in cultures supplemented with 0.0023 mol/L NaHCO₃. Furthermore, when NaCl (0.457 mol/L and 0.469 mol/L), NaNO₃ (0.082 mol/L) and NaHCO₃ (0.0022 mol/L and 0.0023 mol/L) were individually introduced to the culture, cellulose yield increased up to five times compared to the control group.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04329-y.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 6","pages":"180"},"PeriodicalIF":2.6,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12095769/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144141087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}