3 Biotech最新文献_第7页

Investigating the therapeutic effects of Shenzhiling oral liquid on Alzheimer's disease: a network pharmacology and experimental approach. 研究神芝灵口服液对阿尔茨海默病的治疗作用：一种网络药理学和实验方法。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-17 DOI: 10.1007/s13205-024-04181-6

Gaofeng Qin, Rongqiang Song, Jingyi Sun, Bing Chen, Zhe Liu, Lei Han, Baoliang Sun, Chen Li

{"title":"Investigating the therapeutic effects of Shenzhiling oral liquid on Alzheimer's disease: a network pharmacology and experimental approach.","authors":"Gaofeng Qin, Rongqiang Song, Jingyi Sun, Bing Chen, Zhe Liu, Lei Han, Baoliang Sun, Chen Li","doi":"10.1007/s13205-024-04181-6","DOIUrl":"10.1007/s13205-024-04181-6","url":null,"abstract":"There is currently no effective treatment for Alzheimer's disease (AD). This research explored Shenzhiling Oral Liquid (SZLD) against AD by pinpointing crucial elements and understanding its molecular mechanisms through network pharmacology and in vitro experiment. First, we used network pharmacology to screen the main targets and mechanisms of SZLD to improve AD. Then we conducted experiments with Aβ42-induced SH-SY5Y cells to assess SZLD's impact, focusing particularly on apoptotic pathways, thereby uncovering its mechanism of action in AD. Through our analysis, we discovered a notable link between SZLD's effect on AD and apoptosis processes. Specifically, the critical proteins Casapse3 and BCL-2 showed strong correlations in this context. Through systematic data analysis and experimental verification, we unveiled the healing advantages and the foundational molecular mechanisms of SZLD in AD. These findings underscore the promising and compelling potential of targeting the PI3K/Akt signaling pathway and apoptosis with SZLD as a therapeutic strategy to ameliorate AD.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"14"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11652558/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142862999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of novel inhibitors from Rubus ellipticus as anti-leishmanial agents targeting DDX3-DEAD box RNA helicase of Leishmania donovani. 针对多诺瓦利什曼原虫DDX3-DEAD盒RNA解旋酶的新型利什曼病毒抑制剂的鉴定。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-19 DOI: 10.1007/s13205-024-04183-4

Vinita Gouri, Gargi Roy, Akanksha Kanojia, Sumeet Singh, Rohini Muthuswami, Mukesh Samant

{"title":"Identification of novel inhibitors from Rubus ellipticus as anti-leishmanial agents targeting DDX3-DEAD box RNA helicase of Leishmania donovani.","authors":"Vinita Gouri, Gargi Roy, Akanksha Kanojia, Sumeet Singh, Rohini Muthuswami, Mukesh Samant","doi":"10.1007/s13205-024-04183-4","DOIUrl":"10.1007/s13205-024-04183-4","url":null,"abstract":"Visceral leishmaniasis (VL), caused by Leishmania donovani, remains challenging to treat due to severe side effects and increasing drug resistance associated with current chemotherapies. Our study investigates the anti-leishmanial potential of Rubus ellipticus from Uttarakhand, India, with extracts prepared from leaves and stems using ethanol and hexane. Advanced GC-MS analysis identified over 100 bioactive compounds, which were screened using molecular docking to assess their binding to LdHEL-67, a DDX3-DEAD box RNA helicase of L. donovani. Our results spotlighted nine major compounds with high binding energy, which were then further analyzed for ADMET properties and toxicity predictions, demonstrating their promising pharmacokinetic profiles. Among these, clionasterol emerged as the standout compound, displaying superior results in all in silico analyses compared to Amphotericin B (the control). Notably, clionasterol was present in significant proportions across all the mentioned extracts. Subsequent treatment with these extracts led to a remarkable reduction in the intracellular amastigote and axenic amastigote, and promastigote forms of L. donovani and non-toxic to THP-1-derived macrophages. Moreover, the extracts induced apoptotic effects, as evidenced by the fragmentation of parasitic genomic DNA. This study marks a significant leap in developing herbal-based, target-specific inhibitors against VL. Hence, our findings highlight the immense potential of R. ellipticus as a natural treatment for VL.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04183-4.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"18"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11659541/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875831","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

5-Methyltetrahydrofolate and aqueous extract of Spirulina (Arthrospira) ameliorate diabetes and associated complications in STZ-induced diabetic rats. 5-甲基四氢叶酸和螺旋藻水提物可改善stz诱导的糖尿病大鼠的糖尿病及相关并发症。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-18 DOI: 10.1007/s13205-024-04170-9

Ajana Pathikkal, T Krishna Bhaskar, Aparna Prasanthan, P K Haritha, Bijesh Puthusseri, Sudha Rudrappa, Vikas Singh Chauhan

{"title":"5-Methyltetrahydrofolate and aqueous extract of Spirulina (Arthrospira) ameliorate diabetes and associated complications in STZ-induced diabetic rats.","authors":"Ajana Pathikkal, T Krishna Bhaskar, Aparna Prasanthan, P K Haritha, Bijesh Puthusseri, Sudha Rudrappa, Vikas Singh Chauhan","doi":"10.1007/s13205-024-04170-9","DOIUrl":"10.1007/s13205-024-04170-9","url":null,"abstract":"The present study evaluated the effects of 5-methyltetrahydrofolate (5-MTHF) and Spirulina aqueous extract on diabetes. An in silico docking study with select Spirulina bioactive compounds showed strong binding affinities of folates with glucose metabolism-related proteins. In vitro assay showed 5-MTHF's superior inhibitory activity on alpha-amylase compared to folic acid. The protective effect of Spirulina aqueous extract and 5-MTHF were validated in vivo using an STZ-induced diabetic Wistar rat model. Supplementation with Spirulina extract through diet, and a higher dose of 5-MTHF through gavage effectively lowered fasting blood glucose levels and improved oral glucose tolerance and amylase content. Supplementation also countered hyperlipidemia, improved the levels of antioxidant enzymes, and reduced the inflammatory markers. Weight loss prevention, mitigation of kidney enlargement, and normalisation of the histology of the pancreas, kidney, and liver were also observed. The ameliorative effect of a higher dose of 5-MTHF was comparatively superior to Spirulina aqueous extract and a corresponding higher dose of folic acid. An increase in serum folate levels on supplementation with Spirulina aqueous extract suggests Spirulina to be a source of bioavailable folate. The positive effect of Spirulina aqueous extract suggests a potential synergistic role for folate along with its other bioactive phytochemicals. The study highlights the potential ameliorative effects of Spirulina aqueous extract and 5-MTHF as a dietary supplement on diabetes and associated complications.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04170-9.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"15"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

tsRNA-5006c regulates hippocampal neurons ferroptosis to ameliorate perioperative neurocognitive disorders in aged male mice. tsRNA-5006c调节海马神经元铁下垂改善老年雄性小鼠围手术期神经认知障碍。

IF 2.6 4区生物学

3 Biotech Pub Date : 2025-01-01 Epub Date: 2024-12-18 DOI: 10.1007/s13205-024-04176-3

Chuanlin Zhou, Fang Lian, Hejian Li, Fumou Deng

{"title":"tsRNA-5006c regulates hippocampal neurons ferroptosis to ameliorate perioperative neurocognitive disorders in aged male mice.","authors":"Chuanlin Zhou, Fang Lian, Hejian Li, Fumou Deng","doi":"10.1007/s13205-024-04176-3","DOIUrl":"10.1007/s13205-024-04176-3","url":null,"abstract":"The aim of this research is to investigate whether ferroptosis occurs in the pathogenesis of perioperative neurocognitive disorders (PND), and to explore the function and underlying molecular mechanism of tsRNA in the regulation of ferroptosis in PND. A PND aged mice model was established and behavioral changes and ferroptosis occurrence were confirmed. The effect of ferroptosis inhibitor ferrostatin-1 (Fer-1) on PND mice was detected. tsRNA expression profile in PND mice and the effect of tsRNA on ferroptosis in vitro were perfomed. We found that anxious exploration behavior and short-term working memory was declined in PND mice compared with control mice, and the levels of S100β and IL-6 were increased. Meanwhile, hippocampal neurons of PND mice were damaged and accompanied by ferroptosis. Fer-1 can improve cognitive impairment in PND mice, as reflected by improved anxious exploration behavior and short-term working memory, and the levels of S100β and IL-6 were decreased. The expression profile of tsRNA in PND mice is disordered, and the dysregulated tsRNAs were mainly enriched in biologic functions related to neuronal development and ferroptosis. The tsRNA-5006c, identified as a pivotal player, significantly suppressed ferroptosis in primary mice neurons. This study shows for the first time that the pathophysiological process of PND is accompanied by ferroptosis of neurons, and reveals that tsRNA-5006c regulates ferroptosis of hippocampal neurons to ameliorate PND, which is of great significance for the development of new treatment strategies.Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04176-3.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 1","pages":"16"},"PeriodicalIF":2.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11655729/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142875850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutting-edge perspectives on biosurfactants: implications for antimicrobial and biomedical applications. 生物表面活性剂的前沿视角：对抗菌和生物医学应用的影响。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-12-01 Epub Date: 2024-11-11 DOI: 10.1007/s13205-024-04146-9

Kashish Gupta, Varun Kumar Sharma

{"title":"Cutting-edge perspectives on biosurfactants: implications for antimicrobial and biomedical applications.","authors":"Kashish Gupta, Varun Kumar Sharma","doi":"10.1007/s13205-024-04146-9","DOIUrl":"10.1007/s13205-024-04146-9","url":null,"abstract":"Biosurfactants, naturally produced by plants and microorganisms, closely mimic synthetic surfactants in physiochemical properties, making them valuable alternatives in various applications. They serve as antimicrobial agents and play a crucial role in immune regulations. These compounds find wide use in industries like food processing, biodegradation, pharmaceuticals, and naturally present in the skin, brain, lungs, and gut, maintaining membrane permeability for organ health. This review outlines the basic characteristics and classes of biosurfactants (glycolipids, lipopeptides, phospholipids, and glycoproteins) and explores their biomedical importance, emphasizing their anti-adhesive, antimicrobial, and immune-modulating properties. This review aimed to provide outline the fundamental characteristics of biosurfactants and deliver a brief overview of their different classes, including glycolipids, lipopeptides, phospholipids, and glycoproteins. Furthermore, this review also explore their biomedical significance, highlighting their anti-adhesive, antimicrobial, and immune-modulating properties.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"297"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11554964/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cultivation of microalgae Chlorella vulgaris, Monoraphidium sp and Scenedesmus obliquus in wastewater from the household appliance industry for bioremediation and biofuel production. 在家用电器工业废水中培养微藻类小球藻、单胞藻和双胞藻，用于生物修复和生物燃料生产。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-12-01 Epub Date: 2024-11-09 DOI: 10.1007/s13205-024-04142-z

Kelly Lima de Oliveira, José Lucas da Silva Oliveira, Egídia Andrade Moraes, Kelma Maria Dos Santos Pires Cavalcante, Mona Lisa Moura de Oliveira, Carlúcio Roberto Alves

{"title":"Cultivation of microalgae Chlorella vulgaris, Monoraphidium sp and Scenedesmus obliquus in wastewater from the household appliance industry for bioremediation and biofuel production.","authors":"Kelly Lima de Oliveira, José Lucas da Silva Oliveira, Egídia Andrade Moraes, Kelma Maria Dos Santos Pires Cavalcante, Mona Lisa Moura de Oliveira, Carlúcio Roberto Alves","doi":"10.1007/s13205-024-04142-z","DOIUrl":"https://doi.org/10.1007/s13205-024-04142-z","url":null,"abstract":"Microalgae Chlorella vulgaris, Scenedesmus obliquus, and Monoraphidium sp were cultivated in effluent from the household appliance industry as an alternative medium for bioremediation due to the high variability of chemical and biological substances in wastewater. The experiments were carried out using biological effluent (BE), chemical effluent (CE), and a combination of the two (MIX). The results showed a maximum biomass yield of 1056 mg/L (± 0.216) in the BE cultivation of the microalga Scenedesmus obliquus, 969 mg/L (± 0.20) in the BE of the microalga Monoraphidium sp. and 468 mg/L (± 0.46) in the CE of Chlorella vulgaris. In addition, they showed <math><mrow><mi>N</mi> <msubsup><mi>O</mi> <mrow><mn>3</mn></mrow> <mo>-</mo></msubsup> </mrow> </math> removal (100%) in the CE and MIX for cultivation with Chlorella vulgaris and 100% BE and 75% MIX with Monoraphidium sp. For the <math><mrow><mi>P</mi> <msubsup><mi>O</mi> <mrow><mn>3</mn></mrow> <mrow><mn>4</mn> <mo>-</mo></mrow> </msubsup> </mrow> </math> (75.3%, 99% e 97.9%) in the cultures with C. vulgaris BE, CE, and MIX respectively, with Monoraphidium sp. 58% in BE and 42% in CE and MIX. With S. obliquus, 100% removal was observed in all 3 treatments. Metal removal was also observed. The C. vulgaris culture showed lipid contents of 16%, 12%, and 17% for BE, CE, and MIX, respectively. For Monoraphidium sp., 14.5% for BE, 16% for CE, and 14% for MIX. In the culture of S. obliquus, 17%, 15.5%, and 16.5% for BE, CE, and MIX, respectively.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"294"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11550306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antidiabetic, anti-inflammatory, antioxidant, and cytotoxicity potentials of green-synthesized zinc oxide nanoparticles using the aqueous extract of Helichrysum cymosum. 利用腊菊水提取物绿色合成的氧化锌纳米颗粒的抗糖尿病、抗炎、抗氧化和细胞毒性潜力。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-12-01 Epub Date: 2024-11-04 DOI: 10.1007/s13205-024-04125-0

Achasih Q Nkemzi, Kunle Okaiyeto, Omolola Oyenihi, Chinyerum S Opuwari, Okobi E Ekpo, Oluwafemi O Oguntibeju

{"title":"Antidiabetic, anti-inflammatory, antioxidant, and cytotoxicity potentials of green-synthesized zinc oxide nanoparticles using the aqueous extract of Helichrysum cymosum.","authors":"Achasih Q Nkemzi, Kunle Okaiyeto, Omolola Oyenihi, Chinyerum S Opuwari, Okobi E Ekpo, Oluwafemi O Oguntibeju","doi":"10.1007/s13205-024-04125-0","DOIUrl":"10.1007/s13205-024-04125-0","url":null,"abstract":"The current research involved the synthesis of zinc oxide nanoparticles (ZnO-NPs) using an aqueous extract of Helichrysum cymosum shoots, and subsequent characterization via different analytical methods, such as UV-Vis spectroscopy, Scanning electron microscope (SEM), Energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), Transmission electron microscope (TEM), and zeta potential. The biological effects of the ZnO-NPs were then tested against C3A hepatocyte cells and L6 myocyte cell lines via series of analysis, including cytotoxicity, antioxidant, anti-inflammatory, and antidiabetic effect via enzymatic inhibition. The UV-Vis analysis showed a maximum absorption spectrum at 360, and the TEM analysis reveals a spherical and hexagonal structures, with an average dimension of 28.05-58.3 nm, and the XRD reveals a crystalline hexagonal structure. The zeta potential evaluation indicated that the ZnO-NPs are relatively stable at - 20 mV, and the FTIR analysis identified some important functional group associated with phenolics, carboxylic acid, and amides that are responsible for reducing and stabilizing the ZnO-NPs. The synthesized ZnO-NPs demonstrated cytotoxic effects on the cell lines at higher concentrations (125 µg/mL and 250 µg/mL), complicating the interpretation of the results of the inflammatory and antioxidant assays. However, there was a significant (p < 0.05) increase in the inhibitions of pancreatic lipase, alpha-glucosidase, and alpha-amylase, indicating beneficial antidiabetic effects.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"291"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11535088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142589473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cryopreservation of two-celled pollen: a model system for studying the cellular mechanisms of cryoinjury and recovery. 双细胞花粉的冷冻保存：研究冷冻损伤和恢复的细胞机制的模型系统。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-12-01 Epub Date: 2024-11-19 DOI: 10.1007/s13205-024-04140-1

Ravi Gowthami, P E Rajasekharan, Subhash Chander, Muthusamy Shankar, Vartika Srivastava, Anuradha Agrawal

{"title":"Cryopreservation of two-celled pollen: a model system for studying the cellular mechanisms of cryoinjury and recovery.","authors":"Ravi Gowthami, P E Rajasekharan, Subhash Chander, Muthusamy Shankar, Vartika Srivastava, Anuradha Agrawal","doi":"10.1007/s13205-024-04140-1","DOIUrl":"10.1007/s13205-024-04140-1","url":null,"abstract":"Cryopreservation serves as an invaluable technique for safeguarding the genetic diversity of plants and various organisms, while also facilitating fundamental biological research. Despite notable advancements in this field, the cryopreservation of certain cell types and tissues remains challenging, particularly those that exhibit sensitivity to low temperatures. Two-celled pollen is a promising model system for the study of cryopreservation. By exploring the cryopreservation of two-celled pollen, deeper insights can be gained into the cellular and molecular mechanisms of cryoinjury and recovery. This knowledge can be used to develop new and improved cryopreservation protocols for a wider range of cell types and tissues. It is relatively simple, consisting of only two cells, and it is relatively easy to cryopreserve and culture. In addition to its potential for improving cryopreservation technologies, the study of two-celled pollen cryopreservation can also shed light on fundamental biological processes such as cell division, development, and stress tolerance. By unlocking the mysteries of two-celled pollen cryopreservation, we can gain a deeper understanding of nature's inner workings. This article reviews examples of studies that have successfully used two-celled pollen cryopreservation, highlighting key findings and discoveries enabled by this technique as case studies.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"304"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11574238/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142680509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Investigating the role of prognostic mitophagy-related genes in non-small cell lung cancer pathogenesis via multiomics and network-based approach. 更正：通过多组学和基于网络的方法研究预后丝裂吞噬相关基因在非小细胞肺癌发病机制中的作用。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-12-01 Epub Date: 2024-11-20 DOI: 10.1007/s13205-024-04149-6

Prithvi Singh, Gulnaz Tabassum, Mohammad Masood, Saleha Anwar, Mansoor Ali Syed, Kapil Dev, Md Imtaiyaz Hassan, Mohammad Mahfuzul Haque, Ravins Dohare, Indrakant Kumar Singh

引用次数: 0

Swimming upregulates APOL3 through regulating macrophage polarization to inhibit glycolysis and the development of melanoma. 游泳通过调节巨噬细胞的极化来上调 APOL3，从而抑制糖酵解和黑色素瘤的发展。

IF 2.6 4区生物学

3 Biotech Pub Date : 2024-12-01 Epub Date: 2024-11-21 DOI: 10.1007/s13205-024-04150-z

Zhenlei Lyu, Appukutty Mahenderan, Ammu Kutty G K Radhakrishnan, Yit Siew Chin, Chao Yin

{"title":"Swimming upregulates APOL3 through regulating macrophage polarization to inhibit glycolysis and the development of melanoma.","authors":"Zhenlei Lyu, Appukutty Mahenderan, Ammu Kutty G K Radhakrishnan, Yit Siew Chin, Chao Yin","doi":"10.1007/s13205-024-04150-z","DOIUrl":"10.1007/s13205-024-04150-z","url":null,"abstract":"This study investigated the role of swimming exercise in regulating melanoma tumour growth and glycolysis in cancer cells, the specific mechanism involved was also studied. In our study, a murine melanoma tumour model was established to assess the impact of swimming on tumour growth. The mRNA and protein expressions were assessed using qRT-PCR, western blot, and IHC. The metabolic behavior of melanoma cells was examined through lactic acid level measurements and glucose consumption assessments. CCK-8 and colony formation assays were used to detect cell viability and proliferation. ELISA was employed to determine the levels of cytokines secreted by macrophages. The interaction between APOL3 and STAT3 was analyzed by dual luciferase reporter gene and ChIP assays. Our results demonstrated that swimming exercise suppressed melanoma growth in mice by suppressing glycolysis, which might be related to APOL3 upregulation. In addition, downregulation of APOL3 in melanoma was associated with poor prognosis, and APOL3 overexpression markedly suppressed melanoma cell proliferation by reducing glucose uptake and lactate production in vitro. Mechanistically, STAT3 directly down-regulated APOL3 transcription. Swimming upregulated APOL3 by inactivating the IL-6R-STAT3 signaling axis in melanoma cells by inhibiting the secretion of IL-6 by M2 macrophages. As expected, IL-6 secreted by M2 macrophages promoted glycolysis in melanoma cells by reducing APOL3 expression. In summary, swimming inactivated the IL-6R/STAT3 signaling axis in melanoma cells by inhibiting the secretion of IL-6 by M2 macrophages, which could suppress the growth of melanoma in the body by upregulating APOL3 to inhibit glycolysis.","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 12","pages":"307"},"PeriodicalIF":2.6,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11582285/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0