3 Biotech最新文献

{"title":"<i>Streptococcus mutans</i> biofilms in the establishment of dental caries: a review.","authors":"Thangavelu Thayumanavan, B S Harish, Rathinasamy Subashkumar, Karuppusamy Shanmugapriya, Velusamy Karthik","doi":"10.1007/s13205-025-04227-3","DOIUrl":"10.1007/s13205-025-04227-3","url":null,"abstract":"<p><p>Dental caries is considered as the most common and multifactorial disease worldwide, caused by a variety of oral microorganisms like <i>Streptococcus</i> spp., <i>Veillonella</i> spp., <i>Actinomyces</i> spp., <i>Bifidobacterium</i> spp., and <i>Lactobacillus fermentum</i>, which colonize food debris in oral cavities. Of them, <i>Streptococcus mutans</i> is the predominant bacterium and can induce progressive tooth destruction, especially during dentition. The superior characteristics of <i>S. mutans</i>, such as the presence of the cell surface protein P1 and exopolysaccharide-synthesizing enzymes, acid tolerance, biofilm-forming ability mediated by <i>brp</i>A gene, and multidrug resistance, render it a highly virulent pathogen in the etiology of dental caries. Given its significant role in dental caries, extensive research has been conducted over the past few decades, focusing on the development of specific antimicrobial treatments, and other innovative therapeutic approaches. To gain deeper insights into the genetic diversity and epidemiological patterns of <i>S. mutans</i>, various genotypic methods have been developed and successfully employed. By combining the insights gained from genetic studies of <i>S. mutans</i> with the suitable control measures against the biofilm, we can develop innovative and effective strategies for preventing and treating dental caries.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"62"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11828782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cirsiliol suppresses the proliferation of human oral cancer cells by targeting topoisomerase I.","authors":"Ping Xu, Jun Chen, Dongwen Li, Lu Shen, Yangyi Zhang, Ruiting Peng, Yong He","doi":"10.1007/s13205-025-04221-9","DOIUrl":"10.1007/s13205-025-04221-9","url":null,"abstract":"<p><p>Oral cancer poses a significant global health challenge, with increasing incidence rates and substantial morbidity and mortality. This study aimed to investigate the antiproliferative effects of cirsiliol in human oral cancer cells. Results from the MTT cell viability assay showed that cirsiliol significantly (<i>p</i> < 0.05) inhibited the growth of all oral cancer cell lines tested, with the IC₅₀ values ranging from 12 to 25 μM. The lowest IC₅₀ of 12 μM was observed against SCC-1 and SCC-25 cell lines, while the IC₅₀ for normal hTRET-OME cells was 75 μM, approximately 6 times higher than against the oral cancer cells. Further molecular analysis revealed that cirsiliol disrupted cellular morphology in SCC-1 and SCC-25 cells with minor effects on the normal hTRET-OME cells. Annexin V/PI staining indicated that the percentage of SCC-1 and SCC-25 apoptotic cells increased significantly from 4.70 and 5.27% in controls to 31.4 and 35.28% at 24 μM cirsiliol, respectively. This effect correlated with an upregulation of Bax, downregulation of Bcl-2, and increased p53 expression. Nonetheless, the apoptotic effects of cirsiliol were considerably lower in normal hTRET-OME cells. Western blotting together with molecular docking analysis suggested that cirsiliol may inhibit the expression of topoisomerase I. Additionally, wound healing and transwell assays demonstrated that cirsiliol significantly (<i>p</i> < 0.05) suppressed the migration and invasion of SCC-1 and SCC-25 cells. In conclusion, these findings indicate that cirsiliol induces apoptosis in oral cancer cells through the inhibition of topoisomerase I.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"65"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11845633/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143482037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predatory potentials of novel <i>Bdellovibrio</i> isolates against multidrug-resistant and extremely drug-resistant bacterial pathogens of animals and plants.","authors":"Santhanam Selvaraj, Santhalingam Gayathri, Perumal Varalakshmi, Nagan Nagarajan, Rajendran Palaniswami, Balasubramaniem Ashokkumar","doi":"10.1007/s13205-025-04230-8","DOIUrl":"10.1007/s13205-025-04230-8","url":null,"abstract":"<p><p><i>Bdellovibrio</i> and like organisms (BALOs) predate on Gram-negative bacteria selectively, which holds promise as an alternate to combat against multidrug-resistant bacterial pathogens. In this study, two <i>Bdellovibrio</i> strains designated MPR17 and MPR18 were isolated from water samples by double-layer agar technique using <i>P. fluorescens</i> as prey and investigated for their predatory potentials against six pathogenic Gram-negative, MDR and XDR bacteria. Both the BALOs preyed on all the tested bacteria including <i>P. syringae</i>, <i>B. glumae</i>, <i>V. parahaemolyticus</i>, <i>P. mirabilis</i>, <i>M. bovis</i>, and <i>A. schindleri</i> with different killing rates. Against <i>A. schindleri</i>, <i>Bdellovibrio</i> sp. MPR18 produced a maximum of 2.11 × 10⁷ PFU/ml, but MPR17 did not produce any detectable PFUs. TEM analysis of predator-prey interactions recorded different life stages including attachment, bdelloplast formation, prey lysis, and release. Morphologically, both the <i>Bdellovibrio</i> isolates MPR17 and MPR18 were small vibrioid in shape with a long single polar flagellum and measured of about < 500 nm diameter in size, suggesting that they are the smallest ever described <i>Bdellovibrio</i>. Predatory activity was further assessed in liquid co-cultures that evidenced time-dependent decrease in the prey cell density from 24 h with significant reduction at 72 h with different degrees of predation with respective prey bacteria. <i>Bdellovibrio</i> sp. MPR17 and MPR18 exhibited strong bacteriolytic activity in liquid cultures, which was equivalent to the bactericidal activities of standard antibiotics especially for <i>M. bovis</i> (99%), <i>P. syringae</i> (90%) and <i>A. schindleri</i> (90%). The findings of this study signify the potential of novel <i>Bdellovibrio</i> sp. MPR17 and MPR18 as a biocontrol agent with multiple host range to eradicate different MDR and XDR bacterial infections, which could contribute positively for resolving the crisis of antimicrobial resistance.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04230-8.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"69"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11868474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143539768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of multifunctional hydrogels based on tannic acid-coated nanocrystalline cellulose.","authors":"Wancheng Song, Jialin Guan, Wei Wei, Siqi Jia, Zhuojin Li, Xurui Yang, Hao Shi","doi":"10.1007/s13205-025-04232-6","DOIUrl":"10.1007/s13205-025-04232-6","url":null,"abstract":"<p><p>Composite hydrogels are promising for wound healing, but combining strong antimicrobial properties with mechanical performance remains challenging due to potential disruptions in cross-linking. This study presented a one-step method to incorporate tannic acid-coated cellulose nanocrystals (TA@CNC) into polyacrylamide hydrogels. The resulting composite hydrogel exhibited superior mechanical strength, environmental stability, and antimicrobial and antioxidant activities. TA@CNC served as a dynamic reinforcement within the porous network, enhancing mechanical stability. The hydrogel also demonstrated sustained and repeatable adhesion, attributed to the moisture-resistant properties of tannic acid. This work offers valuable insights for the design of multifunctional composite hydrogels, with the developed materials showing great potential for use in medical dressings due to their stretchability, self-adhesion, and antimicrobial and antioxidant properties.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"66"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11846811/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of a chloroquine hydrogel for topical treatment of leishmaniasis in BALB/c mice infected with <i>Leishmania</i> (<i>L.</i>) <i>amazonensis</i>.","authors":"Arlindo Gonzaga Branco Junior, Saara Neri Fialho, Aurileya de Jesus Gouveia, Amália Dos Santos Ferreira, Leandro Do Nascimento Martinez, Welington da Silva Paula do Nascimento, Norton Rubens Diunior Lucas Pejara Rossi, Minelly Azevedo da Silva, Mariana Francy Malaquias Lemke, Karine Modolon Zepon, Luiz Alberto Kanis, Laerth Dos Santos Ribeiro, Carolina Bioni Garcia Teles","doi":"10.1007/s13205-025-04216-6","DOIUrl":"10.1007/s13205-025-04216-6","url":null,"abstract":"<p><p>Leishmaniasis, a challenging disease to treat due to the intracellular location of the parasite and host immune modulation, requires innovative treatment solutions. This study evaluates a chloroquine-based hydrogel for topical use, targeting a localized and less invasive approach to leishmaniasis treatment. Thus, this study aimed at evaluating the skin permeation potential of the antimalarial chloroquine and also to evaluate the less invasive, topical treatment in murine models infected by <i>Leishmania (Leishmania) amazonensis</i>. For this end, a formulation was created, containing chloroquine (CQ), which had its release profile assessed through the ex vivo skin permeation assay using <i>Sus scrofa domesticus</i> skin. Afterward, the susceptibility profile of <i>L.</i> (<i>L.</i>) <i>amazonensis</i> in vivo in BALB/c mice was assessed over a 15-day treatment period. This study highlights the potential of chloroquine hydrogel as a topical treatment for tegumentary leishmaniasis, with significant reductions in parasite load and minimal side effects observed. The formulation demonstrated a 15% permeation rate during 12 h, with 20.26% on the 42nd day. A significant reduction in parasite load was observed in the treatment group. Thus, the results presented here point to the need for further pre-clinical trials with a higher concentration of the drug, other formulations and against other Brazilian species of Leishmania. Future studies should explore higher drug concentrations, alternative formulations, and other Leishmania species to expand the therapeutic potential of this approach.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"54"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11799487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relevance of proteomics and metabolomics approaches to overview the tumorigenesis and better management of cancer.","authors":"Pooja Singh, Yashika W Dhir, Shagun Gupta, Ankur Kaushal, Deepak Kala, Rupak Nagraiik, Naveen K Kaushik, Md Salik Noorani, Abdul R Asif, Bharat Singh, Shahbaz Aman, Sunny Dhir","doi":"10.1007/s13205-025-04222-8","DOIUrl":"10.1007/s13205-025-04222-8","url":null,"abstract":"<p><p>Proteomics and metabolomics, integral combination of OMICs platform are gaining prominence in cancer research to enhance scientific knowledge of bio-molecular interactions occurs in the cellular processes during cancer progression. This approach designed to identify potential tools for addressing the complexities of this multifaceted disease. This analysis focussed on the intricate interplay between proteins and metabolites within cancer cells and their surrounding microenvironment. By reviewing current proteomics and metabolomics studies, we aim to gain invaluable insights into tumour biology, progression, and its implication in therapeutic responses. This study highlights the importance of proteomics and metabolomics in discovering therapeutic targets and diagnostic biomarkers for targeted cancer treatment. Proteomics facilitates the analysis of protein expression, modifications and interactions, exemplified by the identification of HER2 mutations leads to development of breast cancer hence targeted therapies like trastuzumab could be initiated. Metabolomics reveals metabolic alternations such as elevated 2-hydroxyglutarate levels in gliomas linked to cancer progression and treatment resistance. The integration of these approaches clarifies complex signalling network driving oncogenesis and paves the way for innovative cancer therapies, including immune cheque point inhibitors. Proteomics and metabolomics have revolutionised cancer biology by revealing intricate signalling networks, metabolic dysregulations, and unique molecular alterations. This information is crucial for early cancer identification and prognosis, and for designing personalized therapeutic strategies. Innovative technologies like artificial intelligence and high-throughput mass spectrometry further enhance the potential of these studies. Fostering multidisciplinary collaboration and data-sharing is essential for maximising the impact of these approaches to cure as well as better management of the cancer.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"58"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11813842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors associated with tooth loss in patients with stages 3 and 4 periodontitis: a short-term retrospective evaluation of patients.","authors":"See Hyung Lee, Angela Chan, Tia Griffith, Lavanya A Sharma, Seyed Ebrahim Alavi, Nigel Robb, Ajay Sharma","doi":"10.1007/s13205-025-04217-5","DOIUrl":"10.1007/s13205-025-04217-5","url":null,"abstract":"<p><p>This retrospective study analyzed factors associated with tooth loss in patients with stages 3 and 4 periodontitis undergoing non-surgical periodontal therapy. Data from 84 patients treated at Griffith University Periodontal Clinic (2019-2022) were examined, focusing on patient factors such as smoking status, osteoporosis, and age, alongside tooth loss variables. The logistic regression analysis revealed that patients with stage 4 periodontitis had a significantly higher likelihood of tooth loss compared to those with stage 3 (odds ratio [OR] 2.12; 95% confidence interval [CI] 1.13-4.84). Smoking was also identified as a significant risk factor, with smokers showing an OR of 1.69 (95% CI 1.4-3.9) for tooth loss. While no statistically significant relationships were observed for other variables (<i>p</i> > 0.05), patients under 40 years and over 71 years with stage 4 periodontitis exhibited notable tooth loss outcomes (<i>p</i> = 0.003 and <i>p</i> = 0.034, respectively). These findings emphasize the importance of integrating smoking cessation programs into periodontal care and tailoring treatment strategies for high-risk groups. Further longitudinal studies with comprehensive data collection are recommended to enhance the understanding of tooth loss predictors in advanced periodontitis.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04217-5.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"60"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11825966/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143432179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in nanomedicine for the diagnosis and therapy of thyroid disorders.","authors":"Padmavati Sahare, Luis M Ruiz-Manriquez, Brenda Anguiano, Antara Banerjee, Surajit Pathak, Asim K Duttaroy, Gabriel Luna-Bárcenas, Sujay Paul","doi":"10.1007/s13205-025-04234-4","DOIUrl":"10.1007/s13205-025-04234-4","url":null,"abstract":"<p><p>Thyroid diseases, including hypo- or hyperthyroidism and thyroid cancer, are among the most prevalent endocrine disorders that remain challenging to diagnose accurately, and conventional treatments have low therapeutic efficacy. Recently, a wide variety of innovations in the area of nanomedicine, including nanotechnology-based quick and portable diagnostic tools and targeted delivery of pharmaceutics for thyroid disorders, are looking extremely promising. Hence, this review elaborates on the pathogenesis of thyroid disorders and the unique composition, structural attributes, synthesis methodologies, and surface design of nanoplatforms, focusing on the three principal application domains of nanomedicine in thyroid disorders: nanodiagnosis, nanotherapy, and nanotheranostics. Subsequently, cutting-edge studies featuring a series of notable and noteworthy accomplishments are comprehensively summarized, highlighting the intrinsic interaction between nanomedicines and thyroid dysfunctions; particular emphasis is placed on the biosafety of nanomedicines, which seeks to facilitate the future clinical translation of this emerging discipline. This comprehensive review concludes by identifying potential challenges and future perspectives of nanomedicine for the diagnosis and therapy of thyroid disorders.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"67"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11850684/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143514223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biocontrol efficacy of native <i>Metarhizium rileyi</i> (Hypocreales: Clavicipitaceae) isolates against <i>Spodoptera litura</i> (F) (Lepidoptera: Noctuidae) and in silico effect of the secondary metabolites against the virulent proteins of the insect.","authors":"P Duraimurugan, K Sankari Meena, D Neethu Roy, E Bharathi, T Meera Devi, A Chowdappa, K S V P Chandrika","doi":"10.1007/s13205-025-04226-4","DOIUrl":"10.1007/s13205-025-04226-4","url":null,"abstract":"<p><p>This study explores the insecticidal potential of secondary metabolites derived from <i>Metarhizium rileyi</i> against <i>Spodoptera litura</i> through an integrative approach involving genomics, metabolomics, and bioinformatics. Four native isolates of <i>M. rileyi</i> were identified and characterized for their insecticidal efficacy. Among these, the SlMr-DOR isolate exhibited the highest effectiveness, achieving a remarkable 90.0% mortality in laboratory bioassays and 93.3% mortality when tested with its crude extract. Gas chromatography-mass spectrometry (GC-MS) analysis of the SlMr-DOR isolate revealed a diverse profile of bioactive volatile secondary metabolites, including squalene, diethyl phthalate, 4-anilinoquinazoline, vinyl 2-ethylhexanoate, 2-phenyl-3-formyl-pyrrole, chloramphenicol, hentriacontane, phthalic acid derivatives, pentacosane, nonanamide, and eicosanoic acid. Among these metabolites, commercially available squalene and diethyl phthalate were further validated for their insecticidal activity through bioassay studies. Molecular docking analysis demonstrated the strong binding affinity of these metabolites with key <i>S. litura</i> target proteins, including putative chemosensory protein CSP8 and α-amylase, revealing their potential mode of action. The results establish that the secondary metabolites, particularly from the SlMr-DOR isolate, are highly effective against <i>S. litura</i>. This study emphasizes the potential of such metabolites as sustainable and effective alternatives for pest management strategies, contributing to integrated pest control approaches and reducing reliance on synthetic chemical pesticides.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"57"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11806170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential of <i>Streptomyces rochei</i> 8ER183 for poly(lactic acid)-degrading enzyme production, biodegradative capability, and its whole-genome sequence characterization.","authors":"Kanaporn Sujarit, Butsakorn Pannim, Nattawan Kuakkhunthod, Udomlak Uywannang, Chatsuda Sakdapetsiri, Titiporn Panyachanakul, Sukhumaporn Krajangsang, Supattra Phanngoen, Vichien Kitpreechavanich, Thanasak Lomthong","doi":"10.1007/s13205-025-04219-3","DOIUrl":"10.1007/s13205-025-04219-3","url":null,"abstract":"<p><p>Ninety-eight actinomycetes isolates were screened for poly(lactic acid) (PLA)-degrading abilities using a minimal medium supplemented with emulsified PLA as the substrate. The isolate 8ER183 showed PLA degradation ability after incubation at ambient temperature (30 ± 2 °C) for 96 h. The 16S rRNA gene and whole-genome sequencing identified strain 8ER183 as <i>Streptomyces rochei</i>, and the genome size was 8.4 Mbp with an average G + C content of 72.39%. Genome mining revealed 5,689 proteins with functional assignments. The predicted degradation gene involving PLA-degrading enzymes such as protease and lipase was correlated with the phenotypic investigation. The enzymes involved in PLA degradation produced by 8ER183 strain were evaluated as protease and lipase. For enzyme production, cassava chips and peptone at 1.0 and 5.0 g/L yielded the highest PLA-degrading enzyme production (0.49 ± 0.02 U/mL) at 45 °C with pH 8.0. Scaling up enzyme production in a 3.0 L airlift bioreactor enhanced enzyme yield to 2.57 ± 0.12 U/mL, representing 5.25- and 32.12-fold increases compared to the optimized medium in shaking flasks and the unoptimized medium, respectively. The crude enzyme was most active at pH 9.0 and 50 °C. Electron microscopy and infrared spectroscopy revealed significant differences in the physical and chemical structures of PLA film after degradation by crude enzyme. This research identified and characterized a novel PLA-degrading actinomycetes strain as a solution to reduce bioplastic accumulation in the environment and contribute to a more sustainable future.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04219-3.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 3","pages":"55"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11802947/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143381323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}