3 Biotech最新文献

{"title":"Antitumor potential of silver nanoparticles against lung cancer: current trends, scope and relevance.","authors":"Anshika Kamboj, Mayank Raj, Vikas Kumar, Sushil Kumar Upadhyay, Manoj Singh, Ajay Sharma, Anil Kumar Sharma","doi":"10.1007/s13205-025-04502-3","DOIUrl":"https://doi.org/10.1007/s13205-025-04502-3","url":null,"abstract":"<p><p>Lung cancer continues to be a disease that is feared on a global scale due to its high mortality rates. The annual incidence of lung cancer is estimated to be 1.8 million, with approximately 1.6 million fatalities. Conventional treatment regimens are ineffective because they are unable to eradicate lung cancer stem cells (LCSCs). LCSCs are known to be highly resistant to treatment, induce relapse, strengthen metastasis, preserve tumorigenicity, and self-renewal. This demonstrates the necessity of a novel treatment modality that can specifically target lung cancer and its progenitor cells. Nanomaterials (NMs) handle unique challenges with outstanding solutions in a variety of industrial and scientific applications. Silver nanoparticles (AgNPs) are among the most commonly used NMs in drug delivery, medical diagnostics, energy harvesting devices, sensors, lubricants, and bioremediation. Notably, they have demonstrated strong antibacterial, anticancer, and antiviral capabilities in the biomedical sector. The literature analysis reveals a selective cytotoxic impact on cancer cells compared to healthy cells, highlighting its potential utility in cancer treatment and emphasizing the necessity to investigate the potential risk of their use to the environment and human health.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"346"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solvent-dependent metabolite profile and in vitro biological activity of frangipani (<i>Plumeria rubra</i>) leaf extracts.","authors":"Anastasia Wheni Indrianingsih, Anjar Windarsih, Salsa Meidika Asari, Suci Indah Pratiwi, Nadia Udanti Suwanda, Eka Noviana, Eka Rahayu, Tri Wiyono","doi":"10.1007/s13205-025-04533-w","DOIUrl":"https://doi.org/10.1007/s13205-025-04533-w","url":null,"abstract":"<p><p>This study aimed to investigate the influence of different solvents on the extraction of bioactive compounds and the resulting biological activities of <i>P. rubra</i> leaves. Extracts were obtained via maceration using methanol, ethanol, ethyl acetate, and hexane, and evaluated for total phenolic content (TPC), antioxidant activity (via DPPH radical scavenging and β-carotene bleaching), antidiabetic activity (via α-glucosidase inhibition), and antibacterial activity against <i>Staphylococcus aureus</i>, <i>Salmonella typhimurium</i>, and <i>Escherichia coli</i>. The extracts were also analyzed using Fourier-transform infrared (FTIR) and liquid chromatography high-resolution mass spectrometry (LC-HRMS) to identify key functional groups and metabolite profiles. Methanol extract exhibited the highest TPC (482.22 mg GAE/g) and α-glucosidase inhibitory activity (91.23%), while ethanol extract showed superior antioxidant activity. All extracts demonstrated antibacterial effects, with the methanol extract showing the strongest inhibition against <i>S. aureus</i> (10.71 mm). LC-HRMS revealed that hexane extract contains mostly fatty acids and triterpenoids, while the primary compound, ursolic acid, was present in methanol, ethanol, and ethyl acetate at comparable quantities. Further chemometric analysis highlighted distinct metabolite profiles and identified pheophorbide A as a primary discriminant for extract classification. Our findings suggest that <i>P. rubra</i> leaves, particularly when extracted with polar solvents, are a promising source of natural antioxidants, antidiabetic agents, and antimicrobials for applications in pharmaceuticals, nutraceuticals, and functional foods. In addition, the solvent-dependent phytochemical profiles can provide direction for further optimization of solvent composition for targeted metabolite extraction.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04533-w.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"362"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12480336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold-brewed yellow tea showed stronger inhibition on high-fat diet induced mice obesity than that hot brewed, due to higher non-ester catechins content based on metabolomic profile.","authors":"Lianbao Zheng, Yuanhang Yang, Qun Ye, Fang Wang, Xiaohong Zhang, Zhengyu Hou, Chenghui Lu, Baoming Tian, Renjie Liu, Tong Hu, Weihong Zhong","doi":"10.1007/s13205-025-04492-2","DOIUrl":"10.1007/s13205-025-04492-2","url":null,"abstract":"<p><p>This study evaluated the effect of Mogan large-leaf yellow tea (MG-LYT) on preventing obesity. It was found that cold-brewed yellow tea has stronger anti-obesity effects in high-fat diet (HFD)-induced obese mice than hot-brewed tea, due to its higher content of non-esterified catechins, as shown by metabolomic and high-performance liquid chromatography (HPLC) analysis. Notably, epigallocatechin (EGC), a key non-ester catechin, likely enhances pancreatic lipase inhibition through allosteric modulation, which explains the greater effectiveness of cold-brewed tea. Dietary supplementation with cold-brewed MG-LYT significantly reduced HFD-induced obesity and related metabolic disorders in mice, including hepatic steatosis, dyslipidemia, and adipose accumulation, while elevating fecal short-chain fatty acid (SCFA) levels and modulating gut microbiota composition. These findings suggest that cold-brewed yellow tea may be a beneficial daily beverage for preventing obesity, improving metabolic health, and regulating gut microbiota.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"325"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12405141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatoprotective role of verbenone against cyclophosphamide-induced oxidative stress, inflammation, apoptosis, and fibrosis in Swiss albino mice: insights into the involvement of NF-κB, caspase-3, and TGF-β signaling pathways.","authors":"Mohd Wasim, Mansoor Ali Syed, Syed Ehtaishamul Haque","doi":"10.1007/s13205-025-04496-y","DOIUrl":"10.1007/s13205-025-04496-y","url":null,"abstract":"<p><p>Cyclophosphamide (CP), an anti-cancer drug, causes oxidative stress, inflammation, apoptosis and fibrosis in liver. Verbenone (VRB), a bicyclic monoterpene ketone, having antioxidant, anti-inflammatory and anti-apoptotic properties, was selected to investigate its efficiency in reversing CP-induced hepatotoxicity. We hypothesized that VRB having good antioxidant, anti-inflammatory and anti-apoptotic properties, might neutralize the CP-induced toxicity and offer liver protection. Seven groups of mice were formed (<i>n</i> = 6): Vehicle Control, CP 200, VRB 200 + CP, VRB 300 + CP, FF 80 + CP, FF 80 per se, and VRB 300 per se. CP (200 mg/kg, i.p.) was injected once on the seventh day, while VRB (200 and 300 mg/kg, p.o.) and fenofibrate (FF, 80 mg/kg, p.o.) were given daily for 14 days. On 15th day, they were sacrificed and samples for biochemical and histological investigation were taken. CP-treated mice showed an increase in malondialdehyde levels, liver enzymes (AST, ALT, ALP, GGT), inflammatory markers (NF-κB, TNF-α, IL-1β, IL-6), and apoptotic markers (cleaved caspase-3), with a decrease in IL-10 and antioxidant enzymes (SOD, catalase, and glutathione). VRB administration significantly improved the liver function by reducing inflammation, oxidative stress, fibrosis and apoptosis. It restored antioxidant enzymes and hepatocyte structure as confirmed by histopathological and immunohistochemical analyses. Thus, these findings clearly supported our hypothesis and proved that VRB has a strong hepatoprotective effect that counteracts CP-induced liver toxicity through antioxidative, anti-inflammatory, anti-apoptotic, and anti-fibrotic mechanisms. However, further research is needed to be done involving animal cancer models to confirm its potential role and efficiency in reducing CP-induced hepatotoxicity.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"326"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12408882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of probiotic on fecal microbiome of broiler chicken reared at two different high altitude locations.","authors":"Swati, Mayarngam Khansu, Deachan Chuskit, Ashish Kumar Srivastava, Jasdeep Singh, Om Prakash Chaurasia, Lilly Ganju","doi":"10.1007/s13205-025-04446-8","DOIUrl":"10.1007/s13205-025-04446-8","url":null,"abstract":"<p><p>Poultry farming in high altitude like Leh-Ladakh, India is a challenge due to hypobaric-hypoxia environment and probiotic may support gut health, feed efficiency, production and adaptation in chicken. Therefore, the present study was performed to elucidate the role of probiotic on broiler chicken gut microflora from two different high altitude locations; Leh and Chang La. No change was found on the growth of the broiler with probiotic intervention. The fecal microbiota study revealed that the microbial abundance was higher in Chang La samples as compared to Leh samples. At taxonomic level, Chang La samples were dominated by <i>Firmicutes, Proteobacteria, Bacteroides, Actinobacteria</i> phylum whereas in Leh samples, <i>Elusimicrobia</i> was dominant followed by <i>Nitrospirae</i>. Probiotic treatment had minimal effect on chicken gut microflora at the genus and species level. The probiotic effect on gut microflora of Leh broilers was more diverse as compared to the control group. Contrary, to the Chang La group, the probiotic effect had less diverse microbial population as compared to the control group; maintaining the gut microbiome homeostasis of broiler chicken in such extreme high altitude. The microbial functional annotation revealed differences majorly in pathways related to maintenance of cellular energy homeostasis, glycan and carbohydrate metabolisms, regulatory systems, signaling pathways, environmental processing and cell-cell signaling. The findings of the study are an indicative of diversified gut microflora of broiler chicken from two distinct high altitude locations. The study can further be useful to explore the role of different probiotic strains, their doses or delivery methods for the adaptation of these birds at such extreme environments.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04446-8.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"328"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12408439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of platelet glycoprotein VI receptor antagonists and their neuroprotective activity: an in silico, in vitro, and in vivo study.","authors":"Mohammad Aquib Siddiqui, Poorvi Saraf, Sushant Kumar Shrivastava, Sudha Madhavi Penumaka, Debabrata Mandal, Ravi Saini, Abhishek Pathak, Kakarla Ramakrishna, Sairam Krishnamurthy","doi":"10.1007/s13205-025-04530-z","DOIUrl":"https://doi.org/10.1007/s13205-025-04530-z","url":null,"abstract":"<p><p>The present study utilized a drug repurposing approach to identify potential GPVI receptor antagonists among FDA-approved drugs. Computational and molecular dynamics simulations revealed that adapalene and ranolazine exhibit strong binding affinities to GPVI receptors and stabilize GPVI proteins, respectively. Both compounds inhibited collagen-induced platelet aggregation, evidenced by the suppression of Syk tyrosine kinase expression, a marker of platelet activation, via GPVI stimulation, as confirmed through flow cytometry analysis. Further analysis using circular dichroism and Raman spectroscopy indicated that collagen exposure induced conformational changes in the α-helical domains of platelets, which were stabilized upon treatment with adapalene and ranolazine. Moreover, thrombotic events can lead to cerebral cell death due to hypoxia, which may be mitigated by neuroprotective compounds. Adapalene and ranolazine were assessed for their neuroprotective capabilities. The results showed that these compounds exhibited neuroprotective effects in SHSY5Y neuroblastoma cells subjected to oxygen-glucose deprivation/reperfusion (OGD/R) injury, reducing HIF-1α expression, ROS production, lipid peroxidation, and caspase-3 levels, while also improving mitochondrial membrane potential and glutathione levels. Acridine orange and propidium iodide staining studies further confirmed a decrease in apoptosis. In the FeCl₃-induced carotid artery thrombosis model, ranolazine effectively inhibited platelet aggregation by modulating GPVI receptor activity, reducing intracellular calcium levels, and enhancing cAMP signaling. It also suppressed critical platelet activation mediators, including COX-1, TXB₂, and PGE₂, thereby mitigating thrombus formation. Collectively, these results suggest that adapalene and ranolazine may serve as multimodal therapeutic agents with the potential to treat both thrombotic and neurological diseases. Future studies focusing on the adapalene and ranolazine molecular mechanisms, bleeding risk, dose titration, and long-term safety while managing thrombotic disorders have to be investigated.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04530-z.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"357"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12450859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication and characterization of agar-based hydrogel incorporating gelatin-coated silver nanoparticles with mupirocin for diabetic wound therapy.","authors":"Ilakkiya Vadavalli Arumugam, Baby Roselin Rajan Durai, Pranav Ragavendra, Justin Antony, Veintramuthu Sankar","doi":"10.1007/s13205-025-04506-z","DOIUrl":"10.1007/s13205-025-04506-z","url":null,"abstract":"<p><p>Diabetic foot ulcers complicated by infection pose significant challenges in wound care, often resulting in prolonged healing times, increased risk of amputation, and substantial healthcare costs. Innovative treatment strategies involving the integration of bio-polymers and nanoparticles into hydrogel formulations offer promising solutions for managing diabetic wounds. This study focuses on synthesizing agar-based hydrogel containing gelatin-coated silver nanoparticles with mupirocin (GAgNPM). Gelatin was isolated from fish skin through acid (GAC) and alkaline pretreatment (GAL) techniques. In comparison to acid pretreatment extraction, the percentage yield of gelatin was higher with alkaline pretreatment at 16.43 ± 0.5%. The isolated GAL and GAC gelatin was characterized based on pH, viscosity, and gel strength. The GAL exhibited a higher pH and viscosity of 6.51 ± 0.1, and 306 ± 1.0 cps, respectively. The isolated GAL gelatin demonstrated superior characteristics and suitability for requiring optimized formulations. Using Design of Expert software, the optimal concentration for GAgNPM formulation was determined through factorial design and desirability approach. Atomic force microscopy and scanning electron microscopy demonstrated that the GAgNPM exhibited a spherical structure. Further, the in vitro antibacterial assay against <i>Staphylococcus aureus</i> and scratch assay were conducted utilizing a 3T3 fibroblasts cell line. Furthermore, the in vitro antibacterial and scratch assays demonstrated significant (<i>p</i> < 0.05) antibacterial and wound healing properties of the nanocomposite hydrogel. These findings strongly support the potential of agar-hydrogel-incorporated gelatin, and silver nanoparticles and mupirocin as an advanced carrier wound dressing material for diabetic wound treatment.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"334"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12425876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An alternative method for in vitro multiplication and rooting of sugarcane using a PET-based temporary immersion bioreactor.","authors":"María Angélica Fuentes-Torres, José Abel López-Buenfil, Eucario Mancilla-Álvarez, Adriana Contreras-Oliva, Jericó Jabín Bello-Bello","doi":"10.1007/s13205-025-04520-1","DOIUrl":"10.1007/s13205-025-04520-1","url":null,"abstract":"<p><p>Micropropagation of sugarcane is important for obtaining elite plantlets with high genetic and phytosanitary quality. This study aimed to establish an alternative method for the in vitro multiplication and rooting of sugarcane using a temporary immersion bioreactor (TIB) based on polyethylene terephthalate (PET). Different culture systems were employed using PET containers in semisolid culture medium and TIB in horizontal and vertical positions. At six weeks in the multiplication phase, the number and length of shoots, number of leaves per shoot, chlorophyll and carotenoid content, stomatal density, and percentage of closed stomata were evaluated, while at four weeks in the rooting phase, the number and length of shoots, number of leaves per shoot, number and length of roots per explant, chlorophyll and carotenoid content, stomatal density, percentage of closed stomata and plantlet survival rate during acclimatization were evaluated. The results showed the effects of the different culture systems on all variables evaluated for both the multiplication and rooting stages. The TIB-horizontal was the best culture system in multiplication and rooting stages, with a higher number of shoots per explant (29.30 and 13.50 shoots, respectively) and a higher number of leaves per shoot (3.92 and 4.57 leaves, respectively). The highest number of roots per explant (6.89 and 7.51 roots, respectively) and the longest roots were observed in the TIB-horizontal and TIB-vertical systems (4.18 and 4.28 cm in length, respectively), while the highest plantlet survival rates during acclimatization were obtained in the TIB-horizontal and vertical systems (99.5 and 99.0%, respectively). The TIB-horizontal position was the most efficient system for the multiplication and rooting of sugarcane. Culture systems in PET containers are an innovative and sustainable alternative for large-scale sugarcane micropropagation.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"340"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12431986/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovative metabolic reprogramming in rice: unlocking drought resilience through microbial consortia interaction and sustainable agriculture.","authors":"Ajay Tomar, Chitranjan Kumar, Kshitij Parmar, Naeem Khan, Ramji Singh, Sunil Kumar Dwivedi, Durga Prasad","doi":"10.1007/s13205-025-04513-0","DOIUrl":"https://doi.org/10.1007/s13205-025-04513-0","url":null,"abstract":"<p><p>Rice is a global staple food facing climate change challenges, such as drought, salinity, alkalinity, and heat stress. Proteomics, genomics, gene expression, and metabolic changes use adaptation strategies, but translating them into field conditions remains a challenge. This review highlights the role of microbial consortia in drought adaptation, crop resilience, and food security through several drought mitigation strategies. Plants use a \"cry for help\" strategy to restructure their microbiome, alleviate stress, and improve health and nutrition. Understanding the complex feedback between microbes and plants is crucial for future crop drought resilience. Microbial consortia eliminate stress, such as drought stress, by acclimatizing plants to various tactics, enhancing water uptake. Techniques like UPLS/GC detect profiling and plant growth regulators influenced by microbial consortia under water scarcity. Metabolomics can identify secondary metabolites, chemical signaling, and governing systems in plant groups, contributing to drug development and drought tolerance in cereal crops, such as rice and wheat. Key microbial consortia candidates have been identified for nitrogen-fixing bacteria, phosphate-solubilizing bacteria, mycorrhizal fungi, phytohormones, siderophores, and biofortifying crops with nutrients, such as zinc. Future research is needed to understand molecular pathways and identify microbial species that improve drought tolerance. Key challenges include addressing drought effects on plants, understanding plant-microbial consortia functions, and advancing multiomics, synthetic communities (SynComs), and host-mediated microbiome engineering for drought-resilient agriculture.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"343"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433425/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A simplified crude sap-based detection of cucumber mosaic virus in chilli germplasm through reverse transcription recombinase polymerase amplification assay (RT-RPA).","authors":"C H Anusha, A Rajashree, B Parameswari, Vijay Mahanthesh, V Venkataravanappa, P Pranusha, Vinod Kumar Sharma, S K Mangrauthia, V Kavi Sidharthan, B Vidya Sagar, B Bhaskar, L Saravanan, V Celia Chalam","doi":"10.1007/s13205-025-04459-3","DOIUrl":"https://doi.org/10.1007/s13205-025-04459-3","url":null,"abstract":"<p><p>Chilli cultivation is under constant threat by many pests and diseases, and about 35 viruses are known to infect chilli globally. Among the viruses, the cucumber mosaic virus (CMV) is the most significant constraint to chilli production worldwide. Detection of CMV in infected plants, reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) methods are employed frequently. These conventional methods have been time consuming, laborious and expensive for detection of samples in low resource laboratories. In order to provide alternatives for the conventional methods currently various isothermal amplification methods have been employed. The present study exhibits the optimisation of the reverse transcription recombinase polymerase amplification (RT-RPA) assay by eliminating the steps of ribonucleic acid extraction, cDNA conversion, and the use of a thermal cycler. The optimized RT-RPA assay successfully detected CMV at concentrations as low as 10^-10 (100 fg) and 10^-9 dilutions of both RNA and crude sap templates, demonstrating high sensitivity comparable to the routine RT-PCR assay. Specificity tests confirmed that the RT-RPA assay did not exhibit cross-reactivity with other common chilli-infecting viruses, such as chilli leaf curl virus (ChiLCV), chilli veinal mottle virus (ChiVMV), capsicum chlorosis virus (CaCV), and groundnut bud necrosis virus (GBNV). Its ability to function efficiently with crude sap as a template further enhances its field applicability, making it a promising tool for early virus detection in low-resource laboratories and onsite diagnostics. The integration of such isothermal amplification techniques could revolutionize plant virus detection and management strategies, ultimately aiding in the mitigation of crop losses and ensuring food security.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04459-3.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"351"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12440836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}