一种利用pet暂浸式生物反应器进行甘蔗离体增殖和生根的替代方法。

IF 2.9 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2025-10-01 Epub Date: 2025-09-12 DOI:10.1007/s13205-025-04520-1
María Angélica Fuentes-Torres, José Abel López-Buenfil, Eucario Mancilla-Álvarez, Adriana Contreras-Oliva, Jericó Jabín Bello-Bello
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引用次数: 0

摘要

甘蔗的微繁繁殖是获得优良遗传品质和植物检疫品质的重要途径。本研究旨在建立一种基于聚对苯二甲酸乙二醇酯(PET)的临时浸没式生物反应器(TIB)进行甘蔗离体增殖生根的替代方法。在半固态培养基中采用PET容器,在水平和垂直位置采用TIB,采用不同的培养体系。在繁殖期第6周,测定芽数和长度、每枝叶数、叶绿素和类胡萝卜素含量、气孔密度和关闭气孔率;在生根期第4周,测定芽数和长度、每枝叶数、每外植体根数和长度、叶绿素和类胡萝卜素含量、气孔密度、关闭气孔率和适应苗存活率。结果表明,不同的培养制度对各变量的影响均体现在繁殖和生根阶段。在繁殖和生根阶段,tib -水平培养体系是最佳的培养体系,每外植体芽数最高(分别为29.30和13.50个),每枝叶数最高(分别为3.92和4.57个)。植根数最高(分别为6.89根和7.51根),植根最长(分别为4.18 cm和4.28 cm),植根成活率最高(分别为99.5%和99.0%)。tib -水平体位是甘蔗繁殖生根最有效的体位。PET容器培养系统是甘蔗大规模繁殖的创新和可持续的替代方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
An alternative method for in vitro multiplication and rooting of sugarcane using a PET-based temporary immersion bioreactor.

Micropropagation of sugarcane is important for obtaining elite plantlets with high genetic and phytosanitary quality. This study aimed to establish an alternative method for the in vitro multiplication and rooting of sugarcane using a temporary immersion bioreactor (TIB) based on polyethylene terephthalate (PET). Different culture systems were employed using PET containers in semisolid culture medium and TIB in horizontal and vertical positions. At six weeks in the multiplication phase, the number and length of shoots, number of leaves per shoot, chlorophyll and carotenoid content, stomatal density, and percentage of closed stomata were evaluated, while at four weeks in the rooting phase, the number and length of shoots, number of leaves per shoot, number and length of roots per explant, chlorophyll and carotenoid content, stomatal density, percentage of closed stomata and plantlet survival rate during acclimatization were evaluated. The results showed the effects of the different culture systems on all variables evaluated for both the multiplication and rooting stages. The TIB-horizontal was the best culture system in multiplication and rooting stages, with a higher number of shoots per explant (29.30 and 13.50 shoots, respectively) and a higher number of leaves per shoot (3.92 and 4.57 leaves, respectively). The highest number of roots per explant (6.89 and 7.51 roots, respectively) and the longest roots were observed in the TIB-horizontal and TIB-vertical systems (4.18 and 4.28 cm in length, respectively), while the highest plantlet survival rates during acclimatization were obtained in the TIB-horizontal and vertical systems (99.5 and 99.0%, respectively). The TIB-horizontal position was the most efficient system for the multiplication and rooting of sugarcane. Culture systems in PET containers are an innovative and sustainable alternative for large-scale sugarcane micropropagation.

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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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