3 Biotech最新文献

{"title":"Development of a novel, rapid, and accurate recombinase polymerase amplification assay for the identification of the glassy-winged sharpshooter, <i>Homalodisca vitripennis</i> (Hemiptera: Cicadellidae).","authors":"Filomena Ng, Dongmei Li, Chandan Pal, Qing-Hai Fan, Bernard J Phiri, Sherly George, Rebijith K Balan","doi":"10.1007/s13205-025-04511-2","DOIUrl":"10.1007/s13205-025-04511-2","url":null,"abstract":"<p><p>The glassy-winged sharpshooter (GWSS; <i>Homalodisca vitripennis</i>) is a vector for the bacterium <i>Xylella fastidiosa</i>, the causative agent of Pierce's disease. Here, we have developed and optimized an isothermal recombinase polymerase amplification assay (RPA) to detect GWSS based on the Internal Transcribed Spacer 2 (ITS2) region. The assay is 100% specific for GWSS (five target and 12 non-target specimens tested). It can detect specimens collected from diverse geographic regions, and no cross-reactions were observed with non-target cicadellids. The assay can effectively detect the presence of GWSS DNA as low as 3 pg. The RPA assay is tenfold less sensitive than real-time PCR assay but three times faster (less than 15 min for RPA vs. 45 min for real-time PCR). Another major advantage of this newly developed RPA assay is that it can be deployed very easily at the sampling site as it does not require any sophisticated instrumentation.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"338"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12431975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis, characterization of N-doped ZnO@zeolite nanocomposite for enhanced simultaneous adsorptive removal of environmental contaminants.","authors":"S Lakshmi Tulasi","doi":"10.1007/s13205-025-04505-0","DOIUrl":"https://doi.org/10.1007/s13205-025-04505-0","url":null,"abstract":"<p><p>In this study, nitrogen-doped zinc oxide (N-ZnO) nanoparticles supported on zeolite matrix (N-ZnO@Zeolite) nanocomposite were synthesized and its adsorption capability tested against dangerous environmental pollutants such as hexavalent chromium (Cr(VI)), Rhodamine B (RhB), and Congo Red (CR) under single as well as binary component batch adsorption systems. The SEM and XRD results of N-ZnO@Zeolite nanocomposite validated the spherical morphology and effective embedding of N-ZnO particles within the porous zeolite matrix with average crystallite sizes of 32 nm (N-ZnO) and 41 nm (N-ZnO@Zeolite). The EDX analysis confirms the elemental composition and validates nitrogen doping and preservation of the zeolite framework. BET measurements indicate a high rise in surface area for the nanocomposite (29.01 m²/g) than pure N-ZnO (21.26 m²/g), implying increased surface-active sites. The lower photoluminescence intensity in the nanocomposite implies repressed electron-hole recombination, which is beneficial for photocatalytic and adsorptive behavior. Adsorption studies confirm that N-ZnO@Zeolite nanocomposite had considerably greater adsorption capacities compared with N-ZnO for removal of all three contaminants, with the best removal occurring at certain pH conditions: pH 3 for Cr(VI), pH 8 for RhB, and pH 6 for CR. The isotherm study confirms the Langmuir model, with maximum adsorption capacity of 147.62, 139.46, and 210.93 mg/g for Cr(VI), RhB, and CR, respectively, by N-ZnO@Zeolite nanocomposite. The enhanced adsorption capacity is due to higher surface area, greater porosity, stabilization of surface charge, and the synergic effect of the interaction between the zeolite support and N-ZnO. The findings in this paper highlight the use of N-ZnO@Zeolite nanocomposite as a multifunctional efficient adsorbent that is particularly promising for removing multiple co-existent pollutants in complicated wastewater.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"354"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12443654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EIF5A2 acts as a potential marker for prognosis and immunity in human cancers.","authors":"Xuan Zhou, Yifei Sun, Qian Yang, Hong Li, Yanxia Jiang, Fenggang Xiang, Ting Liu","doi":"10.1007/s13205-025-04509-w","DOIUrl":"10.1007/s13205-025-04509-w","url":null,"abstract":"<p><p>EIF5A2, a key member of the EIF family, has not been extensively studied regarding its role and mechanism in pan-cancer. TCGA and GTEx analyses were performed to investigate the differential expression of EIF5A2 in tumors and normal tissues. cBioportal was used to investigate the gene alterations of EIF5A2 in tumors. We used Cox regression and Kaplan-Meier analyses to discuss the impact of EIF5A2 expression on prognosis. Quantitative real-time PCR (qRT-PCR) was used to detect EIF5A2 mRNA levels in 12 cases of fresh liver hepatocellular carcinoma (LIHC) and corresponding adjacent non-tumor tissues. Immunohistochemistry (IHC) was employed to evaluate the expression levels of EIF5A2 in 284 cases of LIHC as well as in adjacent non-tumorous tissues. Furthermore, the study explored the association between EIF5A2 expression and various clinicopathological parameters, along with its prognostic implications. Spearman correlation analysis was used to estimate the relationship between EIF5A2 expression and tumor mutation burden (TMB), microsatellite instability (MSI), and immunologic features. \"pRRophetic\"R package was utilized to obtain the sensitivity of common drugs. The Gene Set Enrichment Analysis (GSEA) was used to study the functional enrichment analysis of EIF5A2-related genes. EIF5A2 was overexpressed in most tumors using TCGA and GTEx databases. Cox regression analysis demonstrated that high EIF5A2 expression was associated with unfavorable overall survival (OS) and disease-specific survival (DSS) in head and neck squamous cell carcinoma (HNSC), brain lower grade glioma (LGG), and LIHC. The frequency of EIF5A2 gene alterations was the highest in lung squamous cell carcinoma (LUSC). EIF5A2 expression was associated with TMB, MSI, and immune cell infiltration in some tumors. We performed IHC and qRT-PCR to evaluate EIF5A2 expression in HCC and normal tissues, and found upregulation of EIF5A2 expression at the mRNA and protein levels in LIHC. There was a correlation between EIF5A2 expression and tumor size, tumor grade, and TNM stage in LIHC. Kaplan-Meier survival suggested that the overexpression EIF5A2 group had unfavorable outcomes in LIHC. EIF5A2 expression was correlated with immune cell infiltration in LIHC. The high EIF5A2 expression group was more sensitive to cisplatin, crizontinib, gemcitabine, and nilotinib in LIHC. High EIF5A2 expression was associated with several pathways, including cell cycle, proteasome, DNA replication, primary immunodeficiency and oocyte meiosis. EIF5A2 may serve as a potential prognostic marker and a latent focus for cancer immunological treatment.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"349"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence in cancer care: revolutionizing diagnosis, treatment, and precision medicine amid emerging challenges and future opportunities.","authors":"Chandrabose Selvaraj, William C Cho, Kulanthaivel Langeswaran, Abdulaziz S Alothaim, Rajendran Vijayakumar, Mani Jayaprakashvel, Deepali Desai","doi":"10.1007/s13205-025-04518-9","DOIUrl":"https://doi.org/10.1007/s13205-025-04518-9","url":null,"abstract":"<p><p>Artificial intelligence (AI) is increasingly being used in oncology to assist early detection, diagnosis, prognosis, treatment planning, and drug discovery. A systematic review is required to integrate evidence across various AI applications in cancer treatment. Systematically assess the use of AI applications in oncology, integrate study findings, highlight methodological issues, and set directions for future research. According to PRISMA guidelines, we searched systematically PubMed, Scopus, Web of Science, and IEEE Xplore between January 2013 and December 2024. Search terms integrated AI-related terms with oncology-related terms. Peer-reviewed original research studies with the use of AI on cancer care in human or human-derived datasets were the eligible studies. Two reviewers independently screened the studies, extracted data, and evaluated the risk of bias with suitable tools. 120 out of 4852 records were included according to inclusion criteria. Applications fell into five clusters: imaging/radiomics, genomics/biomarker discovery, drug discovery/repurposing, clinical decision support, and patient monitoring. Convolutional neural networks were predominant in imaging tasks, whereas ML classifiers were prevalent in genomics. Most of the studies showed improved performance with respect to conventional methods although most of the studies failed to conduct multi-center validation. Heterogeneity of data, interpretability limitations, and integration problems were common issues. AI holds great potential along the cancer care continuum but is at risk of being threatened by issues with data quality, validation, interpretability, and translation to practice. Addressing these issues will require collaboration among disciplines, reporting to standardized guidelines, and large-scale validation studies.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"355"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12443665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CitAB two-component system is involved in pathogenesis regulation in Avian pathogenic <i>Escherichia coli</i>.","authors":"Zhuo Cheng, Yuhang Chen, Kai Ma, Yiqian Yang, Hao Pei, Mei Yu, Fei Shang","doi":"10.1007/s13205-025-04517-w","DOIUrl":"10.1007/s13205-025-04517-w","url":null,"abstract":"<p><p>Avian Pathogenic <i>Escherichia coli</i> (APEC) is a major causative agent of colibacillosis, a severe respiratory disease in birds that leads to significant economic losses in poultry production industry. The two-component systems (TCSs) CitA/CitB regulates metabolism and stress adaptation in <i>Enterobacteriaceae</i>, but its contribution to virulence, particularly in APEC, is undefined. In this study, we investigated the function of the CitA/CitB in APEC by analyzing the transcriptional profile of wild-type strain APEC40 compared to a <i>citAB</i>-deletion mutant. Microarray analysis revealed that CitA/CitB significantly impacts the transcription of numerous genes, including multiple genes associated with biofilm formation and virulence. Phenotypic assays confirmed that inactivation of <i>citAB</i> significantly enhanced biofilm formation capacity and multidrug resistance. Quantitative real-time PCR (qRT-PCR) analysis demonstrated that CitAB negatively regulates expression of key genes involved in these phenotypes, including <i>mglB</i>, <i>fimB</i>, <i>glcB</i>, <i>mblC</i>, <i>ompC</i>, <i>ompW</i>, and <i>pgaB</i>. Collectively, these results demonstrate that the CitA/CitB acts as a negative regulator of biofilm formation, multidrug resistance, and key virulence-associated genes in APEC.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04517-w.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"337"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12431991/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green-engineered synthesis of zinc oxide (ZnO) nanoparticles using <i>Musa paradisiaca</i>: evaluation of antioxidant, antimicrobial, anti-inflammatory, and antihyperglycemic activities.","authors":"Saranya Balasubramaniyam, Thirumalaikumaran Rathinam, Mohanakrishnan Srinivasan, Palanisamy Arulselvan, Suresh Mickymaray, Faiz Abdulaziz Alfaiz","doi":"10.1007/s13205-025-04526-9","DOIUrl":"https://doi.org/10.1007/s13205-025-04526-9","url":null,"abstract":"<p><p>The objective of this study was to synthesize and characterize zinc oxide nanoparticles (ZnO NPs) using <i>Musa paradisiaca</i> leaf extract, an underexplored phytochemical resource for green nanobiotechnology. The biosynthesized ZnO NPs were characterized by UV-Vis spectroscopy, FTIR, XRD, SEM, EDX, and zeta potential measurements, confirming their semi-crystalline nature, colloidal stability, and phytochemical capping. Biological evaluations demonstrated strong, dose-dependent activities: antioxidant effects in DPPH, ABTS, FRAP, H₂O₂, and nitric oxide assays; significant antidiabetic potential through inhibition of α-amylase and β-glucosidase; and anti-inflammatory activity validated by BSA, egg albumin, and HRBC membrane stabilization models. Cytotoxicity testing using the brine shrimp lethality assay indicated a low toxicity profile, suggesting good biocompatibility. Together, these findings highlight <i>M. paradisiaca</i>-derived ZnO NPs as a sustainable, multifunctional nanoplatform with promise for managing oxidative stress-related conditions such as diabetes and inflammation. At the same time, limitations must be acknowledged, as the present data are based on in vitro assays. Future studies should focus on in vivo validation, mechanistic investigations, and clinical translation. Beyond biomedicine, these nanoparticles may also hold potential for applications in food preservation, packaging, and environmental remediation.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"358"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12454858/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145136095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Levonorgestrel-induced modulation of triple negative breast cancer proliferation via glucocorticoid receptor.","authors":"Banashree Bondhopadhyay, Navya Aggarwal, Mukesh Chourasia, Sandeep Sisodiya, Showket Hussain","doi":"10.1007/s13205-025-04447-7","DOIUrl":"https://doi.org/10.1007/s13205-025-04447-7","url":null,"abstract":"<p><p>Indiscriminate use of levonorgestrel, a progestin-only contraceptive, can affect breast cancer subtypes, as it can plausibly bind to steroid receptors other than Estrogen Receptor (ER) and Progesterone Receptor (PR). The ER, PR negative status of TNBC makes it important to explore other receptors of levonorgestrel. In this study glucocorticoid receptor (GR) was observed to show - 83.519 kcal/mol free energy of binding with levonorgestrel. To understand the effect of levonorgestrel on breast cancer cells, cellular assays were performed. In the cell proliferation assays, it was found that ER + PR + Her2- cell line, MCF-7, showed reduced proliferation upon treatment with levonorgestrel for 24 h. Whereas TNBC cell lines (MDA-MB-231 and MDA-MB-468) showed statistically significantly higher proliferation rate. Cellular adhesion assays displayed more adhesion in levonorgestrel-treated MCF-7 cells than in levonorgestrel-treated MDA-MB-231 and MDA-MB-468, as compared to the control. Ki67 (proliferation marker) showed upregulation in MCF-7 control. Levonorgestrel-treated MCF-7 cells demonstrated Ki67 downregulation. In contrast, the treatment triggered upregulation of Ki67 in TNBC cell lines on levonorgestrel treatment. Therefore, the study suggests that in the absence of ER and PR in TNBC, levonorgestrel may potentially show its effect through GR. The study emphasizes the need for further research, as there exist several factors, such as the consumption of oral contraceptive pills like levonorgestrel, which might worsen the status of breast cancer in women.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04447-7.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"361"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12474823/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antitumor potential of silver nanoparticles against lung cancer: current trends, scope and relevance.","authors":"Anshika Kamboj, Mayank Raj, Vikas Kumar, Sushil Kumar Upadhyay, Manoj Singh, Ajay Sharma, Anil Kumar Sharma","doi":"10.1007/s13205-025-04502-3","DOIUrl":"https://doi.org/10.1007/s13205-025-04502-3","url":null,"abstract":"<p><p>Lung cancer continues to be a disease that is feared on a global scale due to its high mortality rates. The annual incidence of lung cancer is estimated to be 1.8 million, with approximately 1.6 million fatalities. Conventional treatment regimens are ineffective because they are unable to eradicate lung cancer stem cells (LCSCs). LCSCs are known to be highly resistant to treatment, induce relapse, strengthen metastasis, preserve tumorigenicity, and self-renewal. This demonstrates the necessity of a novel treatment modality that can specifically target lung cancer and its progenitor cells. Nanomaterials (NMs) handle unique challenges with outstanding solutions in a variety of industrial and scientific applications. Silver nanoparticles (AgNPs) are among the most commonly used NMs in drug delivery, medical diagnostics, energy harvesting devices, sensors, lubricants, and bioremediation. Notably, they have demonstrated strong antibacterial, anticancer, and antiviral capabilities in the biomedical sector. The literature analysis reveals a selective cytotoxic impact on cancer cells compared to healthy cells, highlighting its potential utility in cancer treatment and emphasizing the necessity to investigate the potential risk of their use to the environment and human health.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"346"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433929/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145074108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solvent-dependent metabolite profile and in vitro biological activity of frangipani (<i>Plumeria rubra</i>) leaf extracts.","authors":"Anastasia Wheni Indrianingsih, Anjar Windarsih, Salsa Meidika Asari, Suci Indah Pratiwi, Nadia Udanti Suwanda, Eka Noviana, Eka Rahayu, Tri Wiyono","doi":"10.1007/s13205-025-04533-w","DOIUrl":"https://doi.org/10.1007/s13205-025-04533-w","url":null,"abstract":"<p><p>This study aimed to investigate the influence of different solvents on the extraction of bioactive compounds and the resulting biological activities of <i>P. rubra</i> leaves. Extracts were obtained via maceration using methanol, ethanol, ethyl acetate, and hexane, and evaluated for total phenolic content (TPC), antioxidant activity (via DPPH radical scavenging and β-carotene bleaching), antidiabetic activity (via α-glucosidase inhibition), and antibacterial activity against <i>Staphylococcus aureus</i>, <i>Salmonella typhimurium</i>, and <i>Escherichia coli</i>. The extracts were also analyzed using Fourier-transform infrared (FTIR) and liquid chromatography high-resolution mass spectrometry (LC-HRMS) to identify key functional groups and metabolite profiles. Methanol extract exhibited the highest TPC (482.22 mg GAE/g) and α-glucosidase inhibitory activity (91.23%), while ethanol extract showed superior antioxidant activity. All extracts demonstrated antibacterial effects, with the methanol extract showing the strongest inhibition against <i>S. aureus</i> (10.71 mm). LC-HRMS revealed that hexane extract contains mostly fatty acids and triterpenoids, while the primary compound, ursolic acid, was present in methanol, ethanol, and ethyl acetate at comparable quantities. Further chemometric analysis highlighted distinct metabolite profiles and identified pheophorbide A as a primary discriminant for extract classification. Our findings suggest that <i>P. rubra</i> leaves, particularly when extracted with polar solvents, are a promising source of natural antioxidants, antidiabetic agents, and antimicrobials for applications in pharmaceuticals, nutraceuticals, and functional foods. In addition, the solvent-dependent phytochemical profiles can provide direction for further optimization of solvent composition for targeted metabolite extraction.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04533-w.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"362"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12480336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cold-brewed yellow tea showed stronger inhibition on high-fat diet induced mice obesity than that hot brewed, due to higher non-ester catechins content based on metabolomic profile.","authors":"Lianbao Zheng, Yuanhang Yang, Qun Ye, Fang Wang, Xiaohong Zhang, Zhengyu Hou, Chenghui Lu, Baoming Tian, Renjie Liu, Tong Hu, Weihong Zhong","doi":"10.1007/s13205-025-04492-2","DOIUrl":"10.1007/s13205-025-04492-2","url":null,"abstract":"<p><p>This study evaluated the effect of Mogan large-leaf yellow tea (MG-LYT) on preventing obesity. It was found that cold-brewed yellow tea has stronger anti-obesity effects in high-fat diet (HFD)-induced obese mice than hot-brewed tea, due to its higher content of non-esterified catechins, as shown by metabolomic and high-performance liquid chromatography (HPLC) analysis. Notably, epigallocatechin (EGC), a key non-ester catechin, likely enhances pancreatic lipase inhibition through allosteric modulation, which explains the greater effectiveness of cold-brewed tea. Dietary supplementation with cold-brewed MG-LYT significantly reduced HFD-induced obesity and related metabolic disorders in mice, including hepatic steatosis, dyslipidemia, and adipose accumulation, while elevating fecal short-chain fatty acid (SCFA) levels and modulating gut microbiota composition. These findings suggest that cold-brewed yellow tea may be a beneficial daily beverage for preventing obesity, improving metabolic health, and regulating gut microbiota.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"325"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12405141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}