3 Biotech最新文献

{"title":"Development of CRISRP/Cas9-based TP53-knockout pig muscle stem cells for use in the cultured meat industry.","authors":"Witsanu Srila, Amorn Pangjantuk, Phongsakorn Kunhorm, Nipha Chaicharoenaudomrung, Parinya Noisa","doi":"10.1007/s13205-025-04225-5","DOIUrl":"10.1007/s13205-025-04225-5","url":null,"abstract":"<p><p>Muscle satellite cells (MSCs) are essential for cultured meat production although their restricted lifespan and diminished stemness during prolonged culture pose significant limitations. This study established immortalized porcine MSCs using <i>TP53</i> gene deletion with the CRISPR/Cas9 technology. Several <i>TP53</i>-knockout (KO) clones were generated, exhibiting indel alterations and monoallelic and biallelic deletions. These clones exhibited markedly prolonged cellular lifespans compared to wild-type cells, overcoming the constraints of senescence. The growth rates and the proliferation marker (ki67) gene expression (passage 20) in the <i>TP53</i>-KO clones were dramatically elevated compared to the WT cells. All <i>TP53</i>-KO clones demonstrated a loss of stemness, proliferation, and muscle differentiation marker gene expression during long-term cell culture except for Desmin expression in the <i>TP53</i>-KO 42 clone. Immortalized <i>TP53</i>-KO clones maintained the ability to express muscle-specific protein markers compared to wild-type cells. Moreover, all clones had non-tumorigenic behavior, except <i>TP53</i>-KO clones 41 and 42, which displayed tumorigenic potential. <i>TP53</i>-KO clones demonstrated enhanced myogenic differentiation efficiency after multiple passages in comparison to wild-type cells. The results highlight the potential of <i>TP53</i>-KO MSCs as a cellular resource for future cultured meat production.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04225-5.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"92"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920550/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triple regulation of oxidative-acetylation cycling pathways in COPD glucocorticoid resistance by HuaTanJiangQi capsules.","authors":"Jing Wu, Sen Liu, QiQi Song, FuLin Tao, WenTao Zhu, FuPing Zhuang, Wei Fang, ZeGeng Li, DianLei Wang","doi":"10.1007/s13205-025-04249-x","DOIUrl":"10.1007/s13205-025-04249-x","url":null,"abstract":"<p><p>Glucocorticoid (GC) resistance in chronic obstructive pulmonary disease (COPD) induced by long-term smoking, significantly reduces the quality of life of patients. The complex interaction between antioxidants and acetylation is an important factor that contributes to the slow progression of treatment. This study highlights the development of GC resistance in COPD through 4-hydroxynonenal (4-HNE), multidrug resistance-associated protein 1 (MRP1), histone deacetylase 2 (HDAC2), and nuclear related factor 2 (Nrf2), using enzyme-linked immunosorbent assays, western blotting, and siRNA silencing. Our results suggest that long-term exposure to cigarette smoke can increase 4-HNE toxicity via reactive oxygen species (ROS)-induced lipid peroxidation and decrease the expression of MRP1, histone HDAC2, and Nrf2. Together, these molecules form and enhance the cyclic resistance pathway in COPD, including MRP1 reducing 4-HNE efflux, 4-HNE down-regulating HDAC2 expression by oxidation, HDAC2 reducing Nrf2 transcription by deacetylation, and Nrf2 reducing MRP1 expression through acetylation. The HuaTanJiangQi Capsule (HTJQ) reduces GC resistance via a triple regulatory pathway by enhancing the activity of HDAC2, promoting the transcription of Nrf2, up-regulating the expression of MRP1, and reducing lipid peroxidation induced by ROS. Thus, this cyclic mechanism of GC resistance in COPD may open new avenues for robust therapies using HTJQ.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"72"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11883076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143584291","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Beyond the genome: unveiling tissue-specific non-coding RNAs in clove (<i>Syzygium aromaticum L.</i>).","authors":"Nitesh Kumar Sharma, Dwijesh Chandra Mishra, Baibhav Kumar, Sudhir Srivastava, Krishna Kumar Chaturvedi, Awani Kumar Singh, Sharanbasappa D Madival, Neeraj Budhlakoti, Girish Kumar Jha","doi":"10.1007/s13205-025-04251-3","DOIUrl":"10.1007/s13205-025-04251-3","url":null,"abstract":"<p><p>Clove (<i>Syzygium aromaticum</i>), valued for its role in food preservation and medicine, has recently drawn research interest for its noncoding RNAs (ncRNAs). This study discovers 3274 long noncoding RNAs (lncRNAs) and 2404 circular RNAs (circRNAs) from publicly available RNAseq data. We identified the regulation of 834 genes through miRNA-lncRNA-mRNA network interactions. Additionally, 35 lncRNAs were predicted as precursors for 17 microRNAs (miRNAs), highlighting their role in post-transcriptional regulation. Tissue-specific analysis of circRNAs revealed their interaction with 1047 miRNAs and competing for binding sites on 2382 messenger RNAs (mRNAs). These results underscore their involvement in complex regulatory networks. To support further research and development, we developed SaroNcRDb (http://backlin.cabgrid.res.in/saroncrdb/), a web resource providing detailed insights into the types, chromosomal locations, tissue distributions, and interactions of identified ncRNAs. The findings pave the way for future studies to harness the regulatory roles of ncRNAs in improving Clove's agronomic traits and secondary metabolite production.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"81"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11891123/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"POGZ targeted by LINC01355/miR-27b-3p retards thyroid cancer progression via interplaying with MAD2L2.","authors":"Jiancan Lu, Xinglu Zhou, Hongling Zhu, Mei Zou, Lianyong Liu, Xiangqi Li, Mingjun Gu","doi":"10.1007/s13205-025-04231-7","DOIUrl":"10.1007/s13205-025-04231-7","url":null,"abstract":"<p><p>Despite the high morbidity of thyroid cancer (THCA), the underlying molecular pathology remains elusive. That autism-associated protein POGZ has recently been involved in tumorigenesis intrigues us exploring its relevant molecular regulatory network in THCA. Clinical characteristics and intermolecular relationships were dissected by bioinformatics. Interaction between POGZ and MAD2L2 was examined by Co-IP assay. Targeting relationships between miR-27b-3p and POGZ/LINC01355 was verified by sequence prediction and dual-luciferase reporter detection. Cellular effects of genes were assessed by CCK-8 assay, clone formation assay, and Transwell assay, and further confirmed by a tumor-bearing nude mice model. Our results demonstrated a decrease in POGZ expression in THCA tissues and cell lines, and an interaction between POGZ and MAD2L2 protein. POGZ inhibited both the proliferation and motility of THCA cells, with these effects being reversed upon MAD2L2 silencing. LINC01355 exhibited low expression level and a positive correlation with POGZ in THCA. Both miR-27b-3p and LINC01355 were identified as regulators of POGZ through targeting. Elevated miR-27b-3p suppressed POGZ expression. LINC01355 promoted POGZ and counteracted the inhibitory effects of miR-27b-3p. Furthermore, miR-27b-3p increased the proliferation and motility of THCA cells, an effect that was blocked by LINC01355. At the animal level, POGZ, LINC01355, and MAD2L2 all attenuated tumor growth in THCA. Collectively, POGZ restrains THCA growth by interacting with MAD2L2 protein, and POGZ modulation involves a complex interplay orchestrated by LINC01355-targeted miR-27b-3p. By reporting the first POGZ-focused ceRNA network involving noncoding RNA in THCA, our study paves the way for exploring POGZ-related pathways and developing new therapeutic strategies in cancer.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04231-7.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"79"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11890915/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143603336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ecotoxicity of fungal-synthesized silver nanoparticles: mechanisms, impacts, and sustainable mitigation strategies.","authors":"Himanshu Jangid, Gaurav Kumar","doi":"10.1007/s13205-025-04266-w","DOIUrl":"10.1007/s13205-025-04266-w","url":null,"abstract":"<p><p>The review investigates the ecotoxicological implications of fungal-synthesized silver nanoparticles (AgNPs), focusing on their behavior, transformations, and impacts across aquatic and terrestrial ecosystems. Advanced techniques, such as Single-Particle ICP-MS and Nanoparticle Tracking Analysis, reveal the persistence and biotransformation of AgNPs, including silver ion (Ag⁺) release and reactive oxygen species (ROS) generation. The review highlights species-specific bio-accumulation pathways in algae, soil microbes, invertebrates, and vertebrates, along with the limited biomagnification potential within trophic levels. Long-term exposure to AgNPs leads to reduced soil fertility, altered microbial communities, and inhibited plant growth, raising significant ecological concerns. Sustainable mitigation strategies, including bioremediation and advanced filtration systems, are proposed to reduce the environmental risks of AgNPs. This comprehensive analysis provides a framework for future ecological studies and regulatory measures, balancing the technological benefits of fungal-synthesized AgNPs with their environmental safety.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"101"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11953517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced therapeutic approach for vaginal candidiasis: chitosan nanoparticulate thermoreversible in situ gels for sustained clotrimazole delivery.","authors":"Veintramuthu Sankar, Rajendran Selvakumar, R Narmadha, V G Jaishree","doi":"10.1007/s13205-025-04240-6","DOIUrl":"10.1007/s13205-025-04240-6","url":null,"abstract":"<p><p>This study aimed to develop and evaluate clotrimazole (CLZ)-loaded chitosan (CS) nanoparticles in a thermoreversible in situ gel for treating vaginal candidiasis (VC). Chitosan nanoparticles (CS-NPs) were prepared using ionotropic gelation with optimization through the design of experiments (DoE), considering factors such as chitosan pH, sodium tripolyphosphate (TPP) pH, the ratio of chitosan to TPP, and drug. Under optimal conditions (pH of CS, TPP, CS: TPP, and drug at 2, 2, 4:1, and 10 mg), nanoparticles exhibited desirable properties: particle size of 101.7 nm, polydispersity index (PDI) of 0.108, zeta potential of 35.4, and encapsulation efficiency of 98.36%. Thermoreversible in situ gels incorporating poloxamer (PXM) 407 and 188 were produced via the cold method and evaluated for mechanical and physicodynamic properties. It was found that nanoparticulate thermoreversible gel (NTG) prepared with 24% PXM 407, 4% PXM 188, 0.5% HPMC E-50, or 0.5% chitosan is suitable for vaginal administration, since it fulfills the in situ gel characteristics such as pH (4.7), gelation temperature and time (36 ℃ ± 0.2 and 4 ± 0.2 min), and viscosity (2690 cP (centipoise) at 25 ℃ and 15,600 cP at 37 ℃). In vitro release studies for the developed formulation showed 98% drug release over 72 h, with an extended residence time compared to the marketed formulation. In vitro antifungal and cytocompatibility studies revealed that the developed NTG was effective against VC and free from cytotoxicity.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"71"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11876479/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel green synthesis of silver nanoparticles using carotenoid extracted from <i>Kocuria</i> sp.: determination of antioxidant, antimicrobial, and anti-human breast cancer activities.","authors":"Fatemeh Taghipour, Shahrzad Shahbazi, Somayeh Reiisi, Leila Shabani","doi":"10.1007/s13205-025-04261-1","DOIUrl":"10.1007/s13205-025-04261-1","url":null,"abstract":"<p><p>In recent decades, nanotechnology has emerged as a promising field with diverse medical applications. Silver nanoparticles (Ag NPs) exhibit several biological activities. The aim of the current study was to investigate antioxidant, antimicrobial, and anti-breast cancer properties of green-synthesized Ag NPs. To achieve this, Ag NPs were synthesized using <i>Kocuria</i> sp., and their successful formation and physicochemical properties were evaluated using UV-vis, FTIR, XRD, DLS, zeta potential analysis, SEM, and TEM. The antioxidant capacity of Ag NPs was evaluated using a DPPH scavenging assay. The antimicrobial effects of the Ag NPs were tested on two Gram-negative bacteria (<i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i>) and two Gram-positive bacteria (<i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i>) using a 96-well plate. HUVEC, MCF7, and MDA-MB-231 cells were treated with varying concentrations of Ag NPs, and cell viability, migration, and apoptosis rates were assessed using MTT, scratch, and flow cytometry assays, respectively. Additionally, qPCR was performed to analyze the expression levels of some genes involved in apoptosis, such as caspases 3, 8, and 9. Characterization techniques confirmed the successful synthesis of pure crystalline structures and spherical Ag NPs. Antioxidant and antimicrobial assays demonstrated the significant antioxidant capacity of the Ag NPs and their antibacterial properties against all tested bacteria. Moreover, in vitro studies indicated that Ag NPs effectively inhibited cell proliferation, suppressed migration, and induced apoptosis, likely owing to the upregulation of caspase 3, 8, and 9 and BCL2 downregulation genes. Our findings suggest that green-synthesized Ag NPs using carotenoids extracted from <i>Kocuria</i> sp. might serve as promising antibacterial and anti-breast cancer agents; however, more in vitro and in vivo investigations are required to elucidate the therapeutic potential of Ag NPs.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"91"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920494/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Siddha medicine and modern neuroscience: a synergistic approach to neurological care.","authors":"Thamizhoviya Gangadharan, Muthuvel Arumugam","doi":"10.1007/s13205-025-04265-x","DOIUrl":"10.1007/s13205-025-04265-x","url":null,"abstract":"<p><p>Neurological disorders burden modern healthcare causing cognitive and emotional issues. The Siddha system offers a promising herbal and non-invasive-based therapy. This present review explores the neuromodulatory and neuroprotective effects of the Siddha system integrating herbal and non-invasive interventions with modern neuroscience for a comprehensive treatment strategy against neurological disorders. A detailed literature review was conducted using sources, such as Google search engine, Web of Science, and published databases, focusing on keywords related to 'Siddha herbal, non-invasive, traditional approach for neurological disorders'. The findings indicate that Siddha-based herbal medicines exhibit significant pharmacological activities, which help slow the progression of neurological diseases. Additionally, Siddha-based non-invasive therapies, such as yoga, meditation, Varma (traditional pressure point therapy), massage, and other external manipulations, have been reported to significantly increase brain activity, improve cognitive function, and enhance the patient's quality of life. The findings suggest that complementing Siddha-based therapy with modern neuroscience could enhance personalized neurological care in patients. Although Siddha has a rich history in traditional medicine, a significant research gap remains in large-scale clinical trials, treatment standardization, pharmacological validation, and mechanistic understanding. Addressing these gaps could enhance clinical utility in contemporary healthcare, ultimately benefitting brain health and overall well-being. Integrating Siddha medicine with modern neuroscience holds promise for advancing neurological care.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"96"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide scan for SNPs and selective sweeps reveals candidate genes and QTLs for milk production and reproduction traits in Indian Kankrej cattle.","authors":"Raja Thiruvothur Venkatesan, Alex Rani, Singh Umesh, Kumar Sushil, Das Achintya Kumar, Pulapet Sowmya, Markkandan Kesavan, Rathod Bharat Singh, H H Panchasara, Singh Amit Kumar, Rani Chhaya","doi":"10.1007/s13205-025-04263-z","DOIUrl":"https://doi.org/10.1007/s13205-025-04263-z","url":null,"abstract":"<p><p>Genome-wide identification and annotation of SNPs and selective sweeps was done in Kankrej cattle using the ddRAD sequencing method. Identified 1,983,581 SNPs and nearly half (48.81%) of the effects were found in intron region. Around 624 SNPs annotated in 215 candidate genes were associated with various milk production and reproduction traits. The degree of heterozygosity as 0.2907 against expected heterozygosity of 0.3216. Identified 300 candidate selective sweeps and functional profiling of genes in selective sweep regions resulted with 20 significant (adj <i>p</i> < 0.05) functions. Functional annotation revealed 53.2% of QTLs for milk association while 15.33% for production association, 10.68% for reproduction association, and 8.4% for exterior association. The functional enrichment analysis revealed the presence of significant QTLs in 14 chromosomes. The QTL for milk protein percentage was identified as the top most significant milk type along with the milk potassium content, milk casein percentage, milk yield, milk fat yield, etc. The interval to first estrus after calving, age at puberty, calving interval, conception rate, and birth index were some of the significant QTLs identified for reproduction traits. Genes related to keratinization indicated the selection signature in relation to environmental stressors contributing to adaptation of animals to tropical climatic condition.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04263-z.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"90"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11909306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploration of potent antimicrobial and antioxidant bioactive compounds of selected medicinal plants against drug-resistant pathogens.","authors":"Basavaraju Marappa, B S Gunashree","doi":"10.1007/s13205-025-04228-2","DOIUrl":"10.1007/s13205-025-04228-2","url":null,"abstract":"<p><p>Antibiotic resistance is an escalating universal health threat in this modern era, driving the need for innovative therapeutic solutions. Medicinal plants extensively utilized in Ayurveda, offer significant promises as antibacterial agents. This study examines the metabolite composition of methanol crude extracts from <i>Datura metel</i> and <i>Phyllanthus amarus</i> and evaluates their antibacterial and antioxidant properties. The metabolite content of the crude extracts from the leaves of selected plants was investigated and characterized using HPLC and GC-MS techniques. The agar well diffusion method was used to evaluate antibacterial activity against eight pathogenic bacterial strains, and antioxidant activity was measured using the DPPH assay. Among the tested crude extracts, <i>Datura metel</i> exhibited the strongest antibacterial activity against <i>Salmonella typhi</i>, <i>Acinetobacter baumannii</i>, <i>Klebsiella pneumoniae</i>, and methicillin-resistant <i>Staphylococcus aureus</i> (MRSA). <i>Phyllanthus amarus</i> showed notable activity against <i>Salmonella typhi,</i> <i>Acinetobacter baumannii</i>, methicillin-susceptible <i>Staphylococcus aureus</i> (MSSA), and <i>Klebsiella pneumoniae</i>. The extracts exhibited antimicrobial activity against the tested organisms, with the average diameter of the zone of inhibition ranging from 19 to 21 mm and the two leaf extracts exhibited varying degrees of antibacterial activity. In antioxidant assays, the <i>Datura metel</i> extract demonstrated superior activity, with 78.3 ± 2% inhibition and an IC50 value of 40.1 ± 4 µg/mL. These findings highlight <i>Datura metel</i> and <i>Phyllanthus amarus</i> as promising sources of natural antibacterial and antioxidants, offering potential applications in managing infectious diseases.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04228-2.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 4","pages":"95"},"PeriodicalIF":2.6,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11928705/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143690722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}