3 Biotech最新文献

{"title":"Purification and biochemical characterization of laccase from <i>Kuehneromyces mutabilis</i>.","authors":"Yifan Dou, Qingguo Yao, Zhuang Li, Xinyi Zhai, Li-An Wang, Jianhua Lv, Jinxiu Zhang","doi":"10.1007/s13205-025-04512-1","DOIUrl":"10.1007/s13205-025-04512-1","url":null,"abstract":"<p><p>A novel laccase was successfully purified from the culture filtrate of <i>Kuehneromyces mutabilis</i> using a combination of ammonium sulfate precipitation and ion exchange chromatography. The purified enzyme, designated as Kmlac, has a molecular weight of approximately 70 kDa. Partial amino acid sequences of Kmlac, obtained via LC-MS/MS analysis, exhibited significant homology with those of laccases derived from other fungal species. The optimal temperature and pH for Kmlac activity were determined to be 60 °C and 4.0, respectively. Kmlac exhibited relatively stable activity at pH 4.0 and temperatures below 40 °C. Notably, Kmlac activity was enhanced in the presence of Co²⁺, Al³⁺, Ni²⁺ and Cu²⁺ ions at appropriate concentrations, while it was strongly inhibited by DTT, L-cysteine, and phenol. The enzyme demonstrated the ability to degrade various dyes over different time intervals during decolorization processes. The Km value for the ABTS substrate was determined to be 0.56 mM. Given these characteristics, the novel laccase from <i>K. mutabilis</i> holds significant potential for industrial applications.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04512-1.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"336"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12431983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Removal of hydrogen sulfide (H₂S) with <i>Thiobacillus denitrificans</i> biofilter: study of the microbial community conducted through 16S rRNA sequencing analysis.","authors":"Huseyin Tombuloglu, Omer Aga, Imane Boudellioua, Ismail Anil, Cevat Yaman, Aleem Qureshi","doi":"10.1007/s13205-025-04514-z","DOIUrl":"https://doi.org/10.1007/s13205-025-04514-z","url":null,"abstract":"<p><p>Hydrogen sulfide (H₂S) emissions from oil and gas operations, sewage treatment facilities, and landfills are challenges to the quality of life. The main objective of this work is to study the effect of different filter packing materials on <i>Thiobacillus denitrificans</i>-mediated lab-scale bioreactor for H₂S removal using 16S rRNA metagenomic sequencing. In this study, the bioreactor column, which has three distinct layers of Ceramic Ball Filter Media (CBFM), Filter Media Ceramic Rings (FMCR), and Filter Bio Balls (FBB), was designed and operated for 60 days. The microbial community samples adhered to the surfaces of the filling materials were investigated using 16S rRNA metagenomic sequencing with paired-end 2 × 150 base reads (Illumina). The results showed that the H₂S gas removal efficiency reached its maximum of 99% by the end of the seventh day, followed by a steady-state pattern. Compared with polypropylene surfaces, ceramic materials successfully hosted the <i>T. denitrificans</i> bacteria. Changing the filter material altered the phylum species diversity of the microcosms on the filter material, as shown by alpha diversity indices (Shannon and Simpson values). The dominant Phylum across all samples, regardless of the treatment and filter material type, was <i>Proteobacteria</i>, followed by <i>Firmicutes</i> and <i>Bacteroidetes</i>. <i>Comamonas thiooxydans</i>, <i>Comamonas testosteron</i>e, <i>Enterococcus faecalis</i>, <i>Staphylococcus aureus, Staphylococcus cohnii, and Mesorhizobium terrae</i> are the most abundant species detected on the filter materials. In addition, changing the filter material causes a substantial alteration of the dominant species in the microcosm of the bioreactor. These findings highlight the critical role of filter material in supporting H₂S-removing microorganisms.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"344"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12433380/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145068855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Composites based on bismuth nanoparticles: antimicrobial and anticancer properties.","authors":"R O Shaikenov, I S Garkushina","doi":"10.1007/s13205-025-04519-8","DOIUrl":"https://doi.org/10.1007/s13205-025-04519-8","url":null,"abstract":"<p><p>Every year millions of people die from bacterial infections and cancer, which is a serious global health problem. The rapid spread of antibiotic resistance among pathogenic strains of bacteria is a particular cause for concern. Bismuth nanoparticles, especially quantum dots, demonstrate high potential in the diagnosis and treatment of diseases due to their unique properties. They have antimicrobial activity both in combination with irradiation and on their own, making them a promising alternative to traditional antibiotics. In addition, bismuth nanoparticles exhibit cytotoxicity toward cancer cells, especially when exposed to infrared or X-ray radiation, making them perspective agents for cancer therapy. This review discusses various types of bismuth-containing nanoparticles, their antimicrobial and anti-tumor properties, as well as the main mechanisms of action associated with the generation of reactive oxygen species and the photo-thermal effect.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"347"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a 'green' wound healing hydrogel rich in apigenin-7-O-glucoside using an ultrasonication-assisted solvent extract of tomatoes.","authors":"Debalina Kundu, Satadal Das, Subhankar Saha, Ketousetuo Kuotsu, Paramita Bhattacharjee","doi":"10.1007/s13205-025-04489-x","DOIUrl":"https://doi.org/10.1007/s13205-025-04489-x","url":null,"abstract":"<p><p>The study focuses on ultrasonication-assisted green solvent extraction of apigenin-7-o-glucoside from whole <i>tomatoes</i> (<i>Solanum lycopersicum</i>) and subsequent formulation of a novel hydrogel for epidermal wound healing. Optimisation of extraction parameters was performed using Box-Behnken design of experiments and predictive modelling by response surface methodology (RSM) as well as artificial neural network (ANN). HPTLC analysis confirmed a yield of 12.08 µg of apigenin-7-o-glucoside/g tomatoes (dwb) in the extract under the optimised extraction conditions (ethanol:water 1:1 ratio, 60 min, 55 °C). The presence of the same in highest abundance was validated by ESI-TOF-MS analysis of the extract. The ANN-based multilayer perceptron model (3-8-1 architecture) exhibited superior predictive accuracy (<i>R</i> ² = 0.99 vis-à-vis 0.86), outperforming RSM, with extraction temperature being the most influential factor. The Extract_Best was truly unique in exhibiting significant antimicrobial activity with low minimum inhibitory concentrations across a broad microbial spectrum, namely, ATCC strains of <i>Pseudomonas aeruginosa</i>, <i>Staphylococcus aureus</i>, <i>Candida albicans,</i> and methicillin-resistant <i>Staphylococcus aureus</i>. The extract was incorporated into a synthetic hydrogel, which displayed uniform morphology, a particle size of 100.99 ± 10.22 nm nm, and notable antimicrobial efficacy. In vitro release profile revealed first-order release kinetics (69% burst release) of the extract-loaded hydrogel. This is reportedly the first shelf-stable hydrogel utilising a novel apigenin-7-o-glucoside-rich green extract of tomatoes with strong antibacterial and antifungal activities and effective wound healing properties, as demonstrated in rabbit models. Human panellists favoured its cosmetic attributes such as colour, odour, spreadability, non-stickiness, and shine, suggesting its potential as a moisturising therapeutic gel safe for human use.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04489-x.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"350"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436250/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction and dual-phase optimization of anticancer doses of selective novel flavonoids from yangmei (<i>Myrica rubra</i>) in CRISPR-KO TRα cell lines of PTC: insights into Traditional Chinese Medicine for targeted therapeutic manufacturing.","authors":"Salauddin Al Azad, Ashfaque Rahman, A K M Helal Morshed, Tahira Panah, Tamanna Naznin Shetu, Yalnaz Naseem, Sabiha Sultana, Tanzila Ismail Ema, Rukaiya Binte Yeasin, Shairin Shoheli, Salsabeel Nahar Taib, Sabbir Hasan","doi":"10.1007/s13205-025-04543-8","DOIUrl":"https://doi.org/10.1007/s13205-025-04543-8","url":null,"abstract":"<p><p>Thyroid hormone receptor alpha (TRα) was assessed as a drug target in papillary thyroid carcinoma (PTC) using flavonoids from <i>Myrica rubra</i>. Variant screening identified 20 mutations (14 missense and 6 nonsense) associated with the development of PTC in human tissues regulated by TRα. In both TCGA and GEO datasets, PTC had elevated TRα expression and hypermethylated promoter sequence in association with poor prognostic factors (acute disease, nodal metastasis) and poor survival. In TRα knock-out PTC cell lines (CAL62, FTC133, 8505C, MB1, T3M5) using SW579 as a control (non-cancer cell line), the MTT assay demonstrated that quercetin had the most cytotoxic effect (IC₅₀: 27.13 μM; IC₈₀: 56.77 μM), followed by myricetin and then apigenin. The T3M5 cell line was the most sensitive to the compounds, which was corroborated by advanced ddPCR, gene sequencing, and western blot data. The quantum mechanical calculations identified quercetin as the most electronically stable flavonoid (HOMO-LUMO gap: 0.027 a.u.), followed by myricetin (0.41 a.u.) and then apigenin (0.167 a.u.). The molecular docking and the MMGBSA analysis showed that quercetin had the highest TRα binding affinity (-9.8 kcal/mol, 5 H-bonds, -101.23 kcal/mol). The molecular dynamics simulations also demonstrated that quercetin could stabilize TRα, which was measured by RMSD (the lowest value was 0.24 nm), RMSF (0.14 nm), Rg (1.66 nm), SASA (68.3 nm²), H-bonds (5.12), and PCA (1.11 nm), followed by myricetin in all respective parameters. To recapitulate, these integrated experimental and computational findings highlight quercetin as a strong cytotoxic inhibitor of PTC cell arrest in selective CRISPR-KO TRα models, ranking above myricetin and apigenin despite their having unique cytotoxicity parameters. <b>Highlights</b> Mutation screening of TRα as a potential therapeutic target for thyroid carcinoma.Extraction and characterization of selective novel flavonoids from <i>Myrica rubra</i>.MTT analysis of quercetin, myricetin, and apigenin on CRISPR-KO TRα cell lines.Quercetin resulted most significantly in all in vitro and in silico parameters, despite myricetin and apigenin also showing unique patterns of cell growth arrest properties.GES (±) cell lines were verified using ddPCR, Sanger sequencing, and Western blotting as Post-CRISPR-Cas9 KO molecular screening.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04543-8.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"365"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12488553/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the improvement of bioactive phytochemicals, antioxidant activities and flavor profiles in soy whey fermented by various native lactic acid bacteria.","authors":"Huilin Li, Jiarong Yang, Zhihong Qiao, Yunping Zhu","doi":"10.1007/s13205-025-04500-5","DOIUrl":"10.1007/s13205-025-04500-5","url":null,"abstract":"<p><p>Soy whey, a tofu production byproduct rich in nutrients, is typically discarded. This study investigated five natural lactic acid bacteria (LAB) strains isolated from spontaneously fermented soy whey (FSW) for their fermentation performance in soy whey medium. The growth characteristics, total phenols and flavonoids compounds, antioxidant activities, and aroma profiles were evaluated. The results demonstrated that all five LAB strains exhibited excellent adaptability in soy whey fermentation. After fermentation, the total phenols and flavonoids compounds increased, total isoflavone glycosides (daidzin, glycitin and genistin) decreased by 38-47%, while their corresponding aglycones (daidzein, glycitein, and genistein) significantly increased to 36.38-40.52 mg/L, resulting in improved bioavailability. A total of 33 volatile compounds were identified, Off-flavors like hexanal (beany odor) decreased, while new esters (ethyl hexanoate and butyl acetate) enhanced the flavor profile. Sensory evaluation showed LAB fermentation improved soy whey's taste, texture and acceptability, with strain 3-1 performing best. Results demonstrate LAB fermentation effectively reduces anti-nutritional factors while enhancing functionality and palatability, supporting fermented soy whey's potential as a functional beverage.</p><p><strong>Supplementary information: </strong>The online version contains supplementary material available at 10.1007/s13205-025-04500-5.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"331"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12411396/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145013727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a novel, rapid, and accurate recombinase polymerase amplification assay for the identification of the glassy-winged sharpshooter, <i>Homalodisca vitripennis</i> (Hemiptera: Cicadellidae).","authors":"Filomena Ng, Dongmei Li, Chandan Pal, Qing-Hai Fan, Bernard J Phiri, Sherly George, Rebijith K Balan","doi":"10.1007/s13205-025-04511-2","DOIUrl":"10.1007/s13205-025-04511-2","url":null,"abstract":"<p><p>The glassy-winged sharpshooter (GWSS; <i>Homalodisca vitripennis</i>) is a vector for the bacterium <i>Xylella fastidiosa</i>, the causative agent of Pierce's disease. Here, we have developed and optimized an isothermal recombinase polymerase amplification assay (RPA) to detect GWSS based on the Internal Transcribed Spacer 2 (ITS2) region. The assay is 100% specific for GWSS (five target and 12 non-target specimens tested). It can detect specimens collected from diverse geographic regions, and no cross-reactions were observed with non-target cicadellids. The assay can effectively detect the presence of GWSS DNA as low as 3 pg. The RPA assay is tenfold less sensitive than real-time PCR assay but three times faster (less than 15 min for RPA vs. 45 min for real-time PCR). Another major advantage of this newly developed RPA assay is that it can be deployed very easily at the sampling site as it does not require any sophisticated instrumentation.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"338"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12431975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145063208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EIF5A2 acts as a potential marker for prognosis and immunity in human cancers.","authors":"Xuan Zhou, Yifei Sun, Qian Yang, Hong Li, Yanxia Jiang, Fenggang Xiang, Ting Liu","doi":"10.1007/s13205-025-04509-w","DOIUrl":"10.1007/s13205-025-04509-w","url":null,"abstract":"<p><p>EIF5A2, a key member of the EIF family, has not been extensively studied regarding its role and mechanism in pan-cancer. TCGA and GTEx analyses were performed to investigate the differential expression of EIF5A2 in tumors and normal tissues. cBioportal was used to investigate the gene alterations of EIF5A2 in tumors. We used Cox regression and Kaplan-Meier analyses to discuss the impact of EIF5A2 expression on prognosis. Quantitative real-time PCR (qRT-PCR) was used to detect EIF5A2 mRNA levels in 12 cases of fresh liver hepatocellular carcinoma (LIHC) and corresponding adjacent non-tumor tissues. Immunohistochemistry (IHC) was employed to evaluate the expression levels of EIF5A2 in 284 cases of LIHC as well as in adjacent non-tumorous tissues. Furthermore, the study explored the association between EIF5A2 expression and various clinicopathological parameters, along with its prognostic implications. Spearman correlation analysis was used to estimate the relationship between EIF5A2 expression and tumor mutation burden (TMB), microsatellite instability (MSI), and immunologic features. \"pRRophetic\"R package was utilized to obtain the sensitivity of common drugs. The Gene Set Enrichment Analysis (GSEA) was used to study the functional enrichment analysis of EIF5A2-related genes. EIF5A2 was overexpressed in most tumors using TCGA and GTEx databases. Cox regression analysis demonstrated that high EIF5A2 expression was associated with unfavorable overall survival (OS) and disease-specific survival (DSS) in head and neck squamous cell carcinoma (HNSC), brain lower grade glioma (LGG), and LIHC. The frequency of EIF5A2 gene alterations was the highest in lung squamous cell carcinoma (LUSC). EIF5A2 expression was associated with TMB, MSI, and immune cell infiltration in some tumors. We performed IHC and qRT-PCR to evaluate EIF5A2 expression in HCC and normal tissues, and found upregulation of EIF5A2 expression at the mRNA and protein levels in LIHC. There was a correlation between EIF5A2 expression and tumor size, tumor grade, and TNM stage in LIHC. Kaplan-Meier survival suggested that the overexpression EIF5A2 group had unfavorable outcomes in LIHC. EIF5A2 expression was correlated with immune cell infiltration in LIHC. The high EIF5A2 expression group was more sensitive to cisplatin, crizontinib, gemcitabine, and nilotinib in LIHC. High EIF5A2 expression was associated with several pathways, including cell cycle, proteasome, DNA replication, primary immunodeficiency and oocyte meiosis. EIF5A2 may serve as a potential prognostic marker and a latent focus for cancer immunological treatment.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"349"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12436671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green synthesis, characterization of N-doped ZnO@zeolite nanocomposite for enhanced simultaneous adsorptive removal of environmental contaminants.","authors":"S Lakshmi Tulasi","doi":"10.1007/s13205-025-04505-0","DOIUrl":"https://doi.org/10.1007/s13205-025-04505-0","url":null,"abstract":"<p><p>In this study, nitrogen-doped zinc oxide (N-ZnO) nanoparticles supported on zeolite matrix (N-ZnO@Zeolite) nanocomposite were synthesized and its adsorption capability tested against dangerous environmental pollutants such as hexavalent chromium (Cr(VI)), Rhodamine B (RhB), and Congo Red (CR) under single as well as binary component batch adsorption systems. The SEM and XRD results of N-ZnO@Zeolite nanocomposite validated the spherical morphology and effective embedding of N-ZnO particles within the porous zeolite matrix with average crystallite sizes of 32 nm (N-ZnO) and 41 nm (N-ZnO@Zeolite). The EDX analysis confirms the elemental composition and validates nitrogen doping and preservation of the zeolite framework. BET measurements indicate a high rise in surface area for the nanocomposite (29.01 m²/g) than pure N-ZnO (21.26 m²/g), implying increased surface-active sites. The lower photoluminescence intensity in the nanocomposite implies repressed electron-hole recombination, which is beneficial for photocatalytic and adsorptive behavior. Adsorption studies confirm that N-ZnO@Zeolite nanocomposite had considerably greater adsorption capacities compared with N-ZnO for removal of all three contaminants, with the best removal occurring at certain pH conditions: pH 3 for Cr(VI), pH 8 for RhB, and pH 6 for CR. The isotherm study confirms the Langmuir model, with maximum adsorption capacity of 147.62, 139.46, and 210.93 mg/g for Cr(VI), RhB, and CR, respectively, by N-ZnO@Zeolite nanocomposite. The enhanced adsorption capacity is due to higher surface area, greater porosity, stabilization of surface charge, and the synergic effect of the interaction between the zeolite support and N-ZnO. The findings in this paper highlight the use of N-ZnO@Zeolite nanocomposite as a multifunctional efficient adsorbent that is particularly promising for removing multiple co-existent pollutants in complicated wastewater.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"354"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12443654/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145111509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effective bioremediation of chromium(VI) by a bacterial consortium for decontamination of Industrial effluents using low-cost substrates.","authors":"Sruthi Kalva, N Narendher, Sriya Reddy Patlolla, J Sreenivasa Rao, Sandeepta Burgula","doi":"10.1007/s13205-025-04508-x","DOIUrl":"https://doi.org/10.1007/s13205-025-04508-x","url":null,"abstract":"<p><p>Low-cost substrates are promising substitutes for bioprocesses that can impact microbial metabolism. In the present study Banana peel extract (BPE) medium as a fermentation medium was compared to commercial optimized LB medium for bacterial consortia (<i>Bacillus cereus</i> SBS1 and <i>Bacillus thuringiensis</i> SBS3) for optimization of Cr (VI) bioremediation. Plackett-Burman design was utilized for optimization of factors, where ammonium chloride, inoculum size, sodium chloride and pH showed significant effects on Cr (VI) reduction. Commercial medium was optimized with 1% NH₄Cl, 0. 7% yeast extract, 0. 3% NaCl, and 2% dextrose) and physical parameters (pH-7.0, temperature-37 °C). The growth of bacteria and percentage of Cr (VI) reduction were analyzed. The Cr (VI) reduction was 80% in BPE medium which was 60% in commercial modified LB medium. The strains optimized in BPE medium when employed for the treatment of industrial effluents in a 5 lt bioreactor showed 90% Cr (VI) reduction. BPE can serve as a suitable medium for effective Cr (VI)) bio reduction at bioreactor level and provide a cost effective and eco-friendly alternative for large scale bioremediation of industrial effluents.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"15 10","pages":"360"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463798/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145184597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}