Profiling of the bacterial community and the degradative capability of newly isolated poly(lactic acid) (PLA)- and poly(butylene succinate) (PBS)-degrading bacteria from coastal samples.

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-10-01 Epub Date: 2025-09-17 DOI:10.1007/s13205-025-04521-0

Ausawadee Phonlamai, Wanthanee Khetkorn, Voranuch Thongpool, Titiporn Panyachanakul, Chanwit Suriyachadkun, Vichien Kitpreechavanich, Chatsuda Sakdapetsiri, Thanasak Lomthong

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引用次数: 0

Abstract

The coastal area of Thailand is a tropical marine environment with high microbial diversity, providing favorable conditions for microorganisms capable of degrading bioplastics. The current study aimed to investigate the bacterial community profiling of four samples collected from a coastal area in Thailand and to isolate the potential thermophilic bacteria with the ability to produce bioplastic-degrading enzymes. Our analysis revealed site-specific predominant genera: Brevibacillus in seawater (64.34 ± 0.27%), Pseudomonas in plastic waste (39.69 ± 3.77%), Pseudoalteromonas in soil (54.83 ± 2.40%), and Psychrobacter in moss rock (41.01 ± 1.67%). The thermophilic bacteria, including 6 poly(lactic acid) (PLA)- and 3 poly(butylene succinate) (PBS)-degrading bacteria, were isolated using a two-step technique in an emulsified polymer medium. These nine isolates were classified into five species across four genera: Brevibacillus gelatini, Microbispora rosea, Actinomadura keratinilytica, Paenibacillus thermoaerophilus, and P. ginsengihumi. Among these, Actinomadura keratinilytica LDF1 and M. rosea BS2-4 exhibited the highest enzymatic activities for PLA and PBS degradation (0.87 ± 0.11 U/mL and 0.31 ± 0.03 U/mL, respectively). Scanning electron microscopy confirmed the degradation capabilities of these strains in culture medium. Crude enzyme from the LDF1 strain demonstrated versatility in degrading various types of PLA, including PLA film, PLA powder, commercial cup, and commercial cutlery, while the strain BS2-4 enzyme effectively degraded PBS in film, powder, commercial cup, and commercial drinking straw. These findings advance our understanding of coastal microbial ecology and also highlight the potential of indigenous bacteria for bioplastic waste management, contributing to sustainable environmental solutions.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04521-0.

查看原文本刊更多论文

海岸带样品中新分离的聚乳酸（PLA）和聚丁二酸丁二酯（PBS）降解菌的菌群特征及降解能力

泰国沿海地区是热带海洋环境，微生物多样性高，为能够降解生物塑料的微生物提供了有利条件。目前的研究旨在调查从泰国沿海地区收集的四个样本的细菌群落特征，并分离出具有产生生物塑料降解酶能力的潜在嗜热细菌。结果表明：海水中的短芽孢杆菌（64.34±0.27%）、塑料废弃物中的假单胞菌（39.69±3.77%）、土壤中的假异单胞菌（54.83±2.40%）和苔藓岩中的冷杆菌（41.01±1.67%）为优势菌属。采用两步法在乳化聚合物培养基中分离出6株聚乳酸(PLA)-降解菌和3株聚琥珀酸丁二烯(PBS)-降解菌。9株分离菌株分为4属5种：明胶短芽孢杆菌、玫瑰微芽孢杆菌、角化放线瘤菌、嗜热芽孢杆菌和人参芽孢杆菌。其中，角化放线瘤菌LDF1和玫瑰分枝杆菌BS2-4对PLA和PBS的降解活性最高（分别为0.87±0.11 U/mL和0.31±0.03 U/mL）。扫描电镜证实了这些菌株在培养基中的降解能力。LDF1菌株的粗酶可以降解各种类型的聚乳酸，包括聚乳酸薄膜、聚乳酸粉末、商业杯和商业餐具，而菌株BS2-4酶可以有效降解膜、粉末、商业杯和商业吸管中的PBS。这些发现促进了我们对沿海微生物生态学的理解，也强调了本地细菌在生物塑料废物管理方面的潜力，有助于可持续的环境解决方案。补充资料：在线版本提供补充资料，网址为10.1007/s13205-025-04521-0。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.