The CitAB two-component system is involved in pathogenesis regulation in Avian pathogenic Escherichia coli.

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-10-01 Epub Date: 2025-09-12 DOI:10.1007/s13205-025-04517-w

Zhuo Cheng, Yuhang Chen, Kai Ma, Yiqian Yang, Hao Pei, Mei Yu, Fei Shang

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引用次数: 0

Abstract

Avian Pathogenic Escherichia coli (APEC) is a major causative agent of colibacillosis, a severe respiratory disease in birds that leads to significant economic losses in poultry production industry. The two-component systems (TCSs) CitA/CitB regulates metabolism and stress adaptation in Enterobacteriaceae, but its contribution to virulence, particularly in APEC, is undefined. In this study, we investigated the function of the CitA/CitB in APEC by analyzing the transcriptional profile of wild-type strain APEC40 compared to a citAB-deletion mutant. Microarray analysis revealed that CitA/CitB significantly impacts the transcription of numerous genes, including multiple genes associated with biofilm formation and virulence. Phenotypic assays confirmed that inactivation of citAB significantly enhanced biofilm formation capacity and multidrug resistance. Quantitative real-time PCR (qRT-PCR) analysis demonstrated that CitAB negatively regulates expression of key genes involved in these phenotypes, including mglB, fimB, glcB, mblC, ompC, ompW, and pgaB. Collectively, these results demonstrate that the CitA/CitB acts as a negative regulator of biofilm formation, multidrug resistance, and key virulence-associated genes in APEC.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04517-w.

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CitAB双组分系统参与禽致病性大肠杆菌的发病调控。

禽致病性大肠杆菌（APEC）是大肠杆菌病的主要病原体，大肠杆菌病是一种严重的鸟类呼吸道疾病，给家禽养殖业造成重大经济损失。双组分系统（TCSs） CitA/CitB调节肠杆菌科的代谢和应激适应，但其对毒力的贡献，特别是在APEC中，尚不明确。在这项研究中，我们通过分析野生型菌株APEC40与citab缺失突变体的转录谱，研究了CitA/CitB在APEC中的功能。微阵列分析显示，CitA/CitB显著影响许多基因的转录，包括与生物膜形成和毒力相关的多个基因。表型分析证实，citAB失活显著增强了生物膜形成能力和多药耐药性。实时荧光定量PCR （qRT-PCR）分析显示，CitAB对这些表型相关的关键基因mglB、fimB、glcB、mblC、ompC、ompW和pgaB的表达呈负调控。综上所述，这些结果表明CitA/CitB是APEC生物膜形成、多药耐药和关键毒力相关基因的负调控因子。补充信息：在线版本包含补充资料，下载地址：10.1007/s13205-025-04517-w。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.