Green synthesis of copper hydroxide nanorods using Solanum pubescens leaf extracts: a promising approach for antimicrobial, antioxidant, and anticancer applications.

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-09-01 Epub Date: 2025-08-04 DOI:10.1007/s13205-025-04404-4

C Neela Mohan, V Ayyadurai, Karuppiah Nagaraj, P Vijaya Kumar, K Raja, P Suganthi, C Muthukumar, A Stalin

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引用次数: 0

Abstract

Copper hydroxide nanorods (Cu(OH)₂ NRs) were successfully produced using ethanolic leaf extracts of Solanum pubescens utilizing a green synthesis technique. UV-Vis, FTIR, XRD, and SEM-EDX analysis revealed the development of orthorhombic, rod-shaped nanoparticles with an average size of ~ 50 nm. Cu(OH)₂ NRs showed significant antibacterial activity, with inhibition zones ranging from 8.55 ± 0.59 mm to 9.80 ± 0.68 mm against bacterial strains like S. aureus and K. pneumoniae, and 6.20 ± 0.43 mm to 7.90 ± 0.55 mm against fungal strains like C. albicans. The DPPH experiment demonstrated dose-dependent antioxidant activity, reaching 87.12 ± 5.87% at 80 µg/ml and an IC₅₀ value of 51.53 µg/ml. Cu(OH)₂ NRs caused up to 42.2% cell death in anticancer experiments against MCF-7 breast cancer cells at the highest dose tested (500 µg/mL). The synthesized Cu(OH)₂ nanorods exhibited strong antimicrobial activity, comparable to conventional antibiotics and significantly superior to the plant extract. Their broad-spectrum efficacy, eco-friendly synthesis, and effectiveness against resistant strains highlight their potential as alternative antimicrobial agents.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04404-4.

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利用短毛龙葵叶提取物绿色合成氢氧化铜纳米棒：一种具有抗菌、抗氧化和抗癌应用前景的方法。

采用绿色合成技术，以短毛茄（Solanum pubescens）叶片乙醇提取物为原料制备了氢氧化铜纳米棒（Cu（OH）₂NRs）。UV-Vis， FTIR， XRD和SEM-EDX分析表明，纳米颗粒的平均尺寸为~ 50 nm。Cu（OH）₂NRs对金黄色葡萄球菌和肺炎克雷伯菌的抑菌范围为8.55±0.59 mm ~ 9.80±0.68 mm，对白色念珠菌的抑菌范围为6.20±0.43 mm ~ 7.90±0.55 mm。DPPH实验显示出剂量依赖的抗氧化活性，在80µg/ml时达到87.12±5.87%，IC₅0值为51.53µg/ml。在MCF-7乳腺癌细胞的抗癌实验中，Cu(OH) 2 NRs在最高剂量（500µg/mL）下导致高达42.2%的细胞死亡。合成的Cu(OH) 2纳米棒具有较强的抗菌活性，与常规抗生素相当，明显优于植物提取物。它们的广谱功效、生态友好的合成以及对耐药菌株的有效性突出了它们作为替代抗菌剂的潜力。补充信息：在线版本包含补充资料，下载地址：10.1007/s13205-025-04404-4。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.