Curcumin improves diabetic vascular aging rats and high glucose-induced cells aging by promoting mitophagy.

IF 2.9 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

3 Biotech Pub Date : 2025-10-01 Epub Date: 2025-09-12 DOI:10.1007/s13205-025-04510-3

Jinlin Wu, Xi Mei, Yong Li, Fang Liu, Dongfang Liu

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引用次数: 0

Abstract

Vascular aging was reported to be closely related to diabetes. This study investigates the inhibitory effects and mechanism of curcumin on diabetic vascular aging by regulating mitophagy through the PINK1 pathway. The diabetic rat model was established by feeding with a high-fat diet and intraperitoneal injection of streptozotocin (STZ), and treated with high-dose (200 mg/kg), low-dose (50 mg/kg) curcumin, or metformin (200 mg/kg), respectively. The role of mitophagy in high glucose (HG)-induced human aortic smooth muscle cells (HASMCs) aging in vitro were investigated. The results indicated that curcumin ameliorated weight loss and improved elevated blood glucose levels in diabetic rats. Curcumin also improved the vascular pathological changes of the common carotid artery, decreased the vascular interstitial collagen fiber and vascular calcium salt deposition, and improved vascular ultrastructure. Furthermore, curcumin significantly decreased ET-1, VCAM-1, and p16 expressions. In addition, curcumin increased the expression of LC3II/I, Beclin1, and PINK1 proteins, while decreasing p62 expression. High-dose curcumin could improve mitochondrial morphology and increase mitochondrial autophagy. Additionally, curcumin increased HASMCs viability and inhibited HG-induced vascular aging by promoting mitophagy in vitro. WB confirmed that LC3II/I, Beclin1, and PINK1 levels were increased, while p62 and p16 levels were decreased. The improvement effect of curcumin on vascular aging was reversed by the mitophagy inhibitor Mdivi-1 or PINK1 siRNA. In conclusion, curcumin alleviates vascular aging in diabetic rats and HG-induced senescence in HASMCs by enhancing mitochondrial autophagy. These results suggest that curcumin has therapeutic potential in alleviating diabetic vascular aging.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04510-3.

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姜黄素通过促进线粒体自噬改善糖尿病大鼠血管老化和高糖诱导的细胞老化。

据报道，血管老化与糖尿病密切相关。本研究探讨姜黄素通过PINK1通路调节线粒体自噬对糖尿病血管衰老的抑制作用及其机制。采用高脂饲料喂养，腹腔注射链脲佐菌素（STZ），并分别给予高剂量（200 mg/kg）、低剂量（50 mg/kg）姜黄素和二甲双胍（200 mg/kg）治疗，建立糖尿病大鼠模型。研究了线粒体自噬在体外高糖诱导的人主动脉平滑肌细胞（HASMCs）衰老中的作用。结果表明，姜黄素可以改善糖尿病大鼠的体重减轻和血糖水平升高。姜黄素还能改善颈总动脉的血管病理改变，减少血管间质胶原纤维和血管钙盐沉积，改善血管超微结构。姜黄素显著降低ET-1、VCAM-1和p16的表达。此外，姜黄素增加了LC3II/I、Beclin1和PINK1蛋白的表达，降低了p62的表达。大剂量姜黄素能改善线粒体形态，增加线粒体自噬。此外，姜黄素通过促进体外有丝分裂，提高HASMCs的活力，抑制hg诱导的血管衰老。WB证实LC3II/I、Beclin1和PINK1水平升高，而p62和p16水平降低。姜黄素对血管衰老的改善作用被线粒体自噬抑制剂Mdivi-1或PINK1 siRNA逆转。综上所述，姜黄素通过增强线粒体自噬来缓解糖尿病大鼠血管老化和hg诱导的HASMCs衰老。提示姜黄素在缓解糖尿病血管老化方面具有一定的治疗潜力。补充信息：在线版本包含补充资料，下载地址：10.1007/s13205-025-04510-3。

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来源期刊

3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

CiteScore

6.00

自引率

0.00%

发文量

314

期刊介绍： 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.