Multicomponent nanofibrous electrospun dressings, evaluated for the healing of full-thickness excision wounds in a porcine model.

IF 2.9 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
3 Biotech Pub Date : 2025-10-01 Epub Date: 2025-09-11 DOI:10.1007/s13205-025-04460-w
Sobha Kota, Ratnakumari Anantha, Radhika Sajja, Pradeep Dumpala
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引用次数: 0

Abstract

The first layer of the advanced wound dressing comprises a 3 mm layer of copper (oxide) nanoparticles synthesized with the leaf extracts of M. oleifera and Musa, mixed with Cochlospermum gossypium and Acacia arabica gums, and coated on the cotton fabric. By electrospinning at 20 kV, a 9-layered nanofibrous framework of the biopolymers cellulose acetate, gelatin, and marine chitosan, impregnated with bioactives such as carbon dots derived from medicinal plants, green synthesized copper nanoparticles, and phytochemicals extracted with acetone and isopropanol was woven. The core, shell and active layer nanofibers of different polymers, with a fiber diameter ranging from 252 nm to 3.1 µm, were grafted onto the CuNP-coated cotton fabric. Four wound dressings viz., WD1 (L)/WD2 (H) with poly l-lysine in low and high concentrations, and WD3 (L)/WD4 (H) with polyethylene oxide in low and high concentrations, were fabricated to improve the piezoelectric behavior. Of them, WD2 and WD4 showed promising biological characteristics of anti-inflammatory, anti-oxidant, and anti-microbial potential. They were endowed with piezoelectric constants of 24.28 pm/V (WD2) and 0.28 nm/V (WD4), and zeta potentials of 57.7 mV (WD2) and 94.6 mV (WD4). Within 8 days of causing full-thickness wounds, the two test items were able to effectively cause quick wound contraction, epithelial tissue regrowth, and elimination of inflammation; by 34 days, normalcy had returned.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-025-04460-w.

多组分纳米纤维电纺敷料在猪模型全层切除伤口愈合中的作用。
先进的伤口敷料的第一层包括一层3毫米厚的铜(氧化物)纳米粒子,该纳米粒子是由油橄榄和芭蕉叶提取物合成的,与棉草和阿拉伯胶混合,并涂在棉织物上。通过在20千伏电流下静电纺丝,将生物聚合物醋酸纤维素、明胶和海洋壳聚糖制成9层纳米纤维框架,并浸渍生物活性物质,如药用植物提取的碳点、绿色合成的铜纳米颗粒以及丙酮和异丙醇提取的植物化学物质。将不同聚合物的芯、壳和活性层纳米纤维(纤维直径为252 nm ~ 3.1µm)接枝到涂覆ccp的棉织物上。制备了低、高浓度聚赖氨酸WD1 (L)/WD2 (H)和低、高浓度聚氧化聚乙烯WD3 (L)/WD4 (H)四种伤口敷料,以改善其压电性能。其中,WD2和WD4具有良好的抗炎、抗氧化、抑菌等生物学特性。压电常数分别为24.28 pm/V (WD2)和0.28 nm/V (WD4), zeta电位分别为57.7 mV (WD2)和94.6 mV (WD4)。在造成全层创面后8天内,两种试验项目均能有效引起创面快速收缩、上皮组织再生、炎症消除;到第34天,一切恢复正常。补充信息:在线版本包含补充资料,提供地址:10.1007/s13205-025-04460-w。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
3 Biotech
3 Biotech Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
6.00
自引率
0.00%
发文量
314
期刊介绍: 3 Biotech publishes the results of the latest research related to the study and application of biotechnology to: - Medicine and Biomedical Sciences - Agriculture - The Environment The focus on these three technology sectors recognizes that complete Biotechnology applications often require a combination of techniques. 3 Biotech not only presents the latest developments in biotechnology but also addresses the problems and benefits of integrating a variety of techniques for a particular application. 3 Biotech will appeal to scientists and engineers in both academia and industry focused on the safe and efficient application of Biotechnology to Medicine, Agriculture and the Environment.
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