Microchimica Acta最新文献_第7页

Smartphone-based colorimetric detection of urease activity in dental plaque for point-of-care screening of Helicobacter pylori infection 基于智能手机的比色法检测牙菌斑中脲酶活性，用于即时筛查幽门螺杆菌感染

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-09-13 DOI: 10.1007/s00604-025-07496-0

Yuhan Wang, Ping Zhang, Chuxian Zhou, Zicong Zheng, Qiang Quan, Guangbo He, Hening Zhai, Qifang Song, Xiaoli Zhang, Heng Li, Lanhuan Peng, Yuqin Liao, Wei Fang, Minmin Li, Jiajie Liang, Yong Tang

{"title":"Smartphone-based colorimetric detection of urease activity in dental plaque for point-of-care screening of Helicobacter pylori infection","authors":"Yuhan Wang, Ping Zhang, Chuxian Zhou, Zicong Zheng, Qiang Quan, Guangbo He, Hening Zhai, Qifang Song, Xiaoli Zhang, Heng Li, Lanhuan Peng, Yuqin Liao, Wei Fang, Minmin Li, Jiajie Liang, Yong Tang","doi":"10.1007/s00604-025-07496-0","DOIUrl":"10.1007/s00604-025-07496-0","url":null,"abstract":"<div><p><i>Helicobacter pylori</i> (<i>H. pylori</i>) is a major pathogen associated with gastric ulcers and gastric cancer, infecting approximately half of the global population. Therefore, developing sensitive, rapid, and cost-effective detection methods for <i>H. pylori</i> is critical for public health. We developed an innovative microscale colorimetric microsensor integrated with smartphone ambient-light sensors for portable, on-site quantification of <i>H. pylori-</i>associated urease activity in dental plaque. This low-cost device (< $ 5 fabrication) employs 3D-printed components and a custom app to measure transmittance changes from urease-catalyzed urea hydrolysis with phenol red, achieving a robust correlation with commercial microplate reader absorbance (<i>R</i><sup>2</sup> = 0.9989). The system demonstrates exceptional Analytical sensitivity, with limits of detection of 9.75 U/L for urease And 1.709 × 10<sup>3</sup> CFU/mL for <i>H. pylori</i>, alongside high reproducibility (CV < 10%) And stability over 14 days. When applied to the on-site detection of dental plaque among volunteers, the smartphone-based colorimetric microsensor exhibited detection results that were highly aligned with <i>H. pylori</i> infections confirmed by the urea breath test. It outperformed commercial test strips And achieved 100% sensitivity, 95.5% specificity, And 97.1% accuracy. This miniaturized, smartphone-based platform offers a promising solution for home self-testing and demonstrates significant potential for enhancing <i>H. pylori</i> preliminary screening at the community level.\u0000</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145050889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disposable paper-based electrochemical sensor for hydroquinone detection in environmental samples using a MXene-CNT hybrid composite 使用MXene-CNT混合复合材料检测环境样品中对苯二酚的一次性纸基电化学传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-09-13 DOI: 10.1007/s00604-025-07485-3

Pramod K. Kalambate, Sharad S. Upadhyay, Shambhavi Singh, Wanida Laiwattanapaisal, Dhanjai

{"title":"Disposable paper-based electrochemical sensor for hydroquinone detection in environmental samples using a MXene-CNT hybrid composite","authors":"Pramod K. Kalambate, Sharad S. Upadhyay, Shambhavi Singh, Wanida Laiwattanapaisal,  Dhanjai","doi":"10.1007/s00604-025-07485-3","DOIUrl":"10.1007/s00604-025-07485-3","url":null,"abstract":"<div><p> This study investigates the electrochemical performance of MXene/multi-walled carbon nanotubes (MWCNT) composite-based sensor and its comparison with MWCNT for the sensitive detection of hydroquinone (HQ) in undiluted water samples. The sensor employs custom-made screen-printed carbon electrodes modified with Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>/MWCNT, allowing for rapid fabrication and integration of the working, reference, and counter electrodes into a compact, portable device. The multilayer MXene provides a large specific surface area, while MWCNT enhances electrical conductivity, enabling clear electrochemical signals even in complex sample matrices. The MWCNT/SPCE and Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub>/MWCNT/SPCE sensors demonstrated excellent sensitivity, achieving low detection limits of 0.055 µM and 0.261 µM, with wide Linear ranges of 3–600 µM and 6–900 µM, respectively. The sensor demonstrates strong selectivity for HQ with minimal interference from coexisting species and requires little to no sample preparation, offering a feasible approach for real-time environmental monitoring.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon nanofiber–enhanced molecular imprinted electrochemical sensor for hypoxanthine detection 用于次黄嘌呤检测的纳米碳纤维增强分子印迹电化学传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-09-12 DOI: 10.1007/s00604-025-07502-5

Canan Armutcu, Sena Pişkin, Erdoğan Özgür, Mustafa Karakaya, M. Emin Çorman, Lokman Uzun

{"title":"Carbon nanofiber–enhanced molecular imprinted electrochemical sensor for hypoxanthine detection","authors":"Canan Armutcu, Sena Pişkin, Erdoğan Özgür, Mustafa Karakaya, M. Emin Çorman, Lokman Uzun","doi":"10.1007/s00604-025-07502-5","DOIUrl":"10.1007/s00604-025-07502-5","url":null,"abstract":"<div><p>A molecularly imprinted electrochemical sensor (MIP) was developed using thymine-functionalized carbon nanofibers (Thy@CNFs) to enable selective detection of hypoxanthine (HYP). The sensor was fabricated by first depositing Thy@CNFs onto a glassy carbon electrode (GCE), followed by electropolymerization of a pyrrole-co-pyrrole-3-carboxylic acid (Py-co-PyCOOH) copolymer in the presence of HYP. Each modification step was characterized using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), and contact angle measurements. Under optimized conditions, the Thy@CNFs-modified MIP sensor (Thy@CNFs/MIP/GCE) exhibited a linear response to HYP concentrations ranging from 1 × 10<sup>−9</sup> to 1 × 10<sup>−8</sup> M, with a detection limit of 1.71 × 10<sup>−10</sup> M. Finally, the sensor was successfully applied to commercial serum and artificial urine sample, achieving recoveries of 99.55% and 100.17%, respectively, demonstrating its accuracy, precision, and practical applicability in real sample analysis.\u0000</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PNIPAAm-functionalized flexible microchip for rapid detection of surface microbial contaminants 用于快速检测表面微生物污染物的pnipaam功能化柔性微芯片

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-09-12 DOI: 10.1007/s00604-025-07539-6

Xi Su, Chuang Ge, Songtao Xiang, Xiaoqin Wu, Yipei Wang, Li Chen, Yi Xu

{"title":"PNIPAAm-functionalized flexible microchip for rapid detection of surface microbial contaminants","authors":"Xi Su, Chuang Ge, Songtao Xiang, Xiaoqin Wu, Yipei Wang, Li Chen, Yi Xu","doi":"10.1007/s00604-025-07539-6","DOIUrl":"10.1007/s00604-025-07539-6","url":null,"abstract":"<div><p>The detection of surface microbial contaminants faces several critical challenges, including disconnection between sampling and detection steps and time-consuming. To overcome these limitations, we developed an innovative integrated microfluidic system that combines bacterial sampling, transfer, concentration, and detection within a single chip platform. The microchip architecture consists of a thermos-responsive flexible sheet incorporating poly(N-isopropylacrylamide) (PNIPAAm) gel micropillar arrays for efficient bacterial capture and thermal-triggered release, and a PDMS cover layer with an optimized canopy-shaped microchannel design for sequential bacterial transport, fluorescent labeling, and in situ concentration/fluorescence detection. To verify the system’s functionality, <i>Staphylococcus aureus</i> (<i>S. aureus</i>) was employed as a model organism for testing the microchip detection platform. Under optimized conditions, the sampling and the elution efficiency of <i>S. aureus</i> on stainless steel sheet were up to 75.6% and 88.4%, respectively. The detection limit of <i>S. aureus</i> was 5.15 × 10<sup>3</sup> CFU/cm<sup>2</sup>, and the entire process was completed in < 45 min. This integrated microfluidic system performs continuous surface microbial sampling and detection, reducing processing time by 32-fold compared to conventional swab-based methods. The platform offers a rapid, integrated, and highly efficient solution for bacterial detection for surface microbial contaminants, with promising applications in food safety, medical hygiene, and environmental monitoring.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CRISPR/Cas13 system-based entropy-driven DNAzyme switch powered DNA walking system for sensitive and direct rotavirus detection 基于CRISPR/Cas13系统的熵驱动DNAzyme开关驱动的DNA行走系统，用于灵敏和直接的轮状病毒检测

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-09-12 DOI: 10.1007/s00604-025-07535-w

Xiaozheng Kang, Fanglu Tian, Xiaogang Liu, Guohui Xiao, Yanyang Cai

{"title":"CRISPR/Cas13 system-based entropy-driven DNAzyme switch powered DNA walking system for sensitive and direct rotavirus detection","authors":"Xiaozheng Kang, Fanglu Tian, Xiaogang Liu, Guohui Xiao, Yanyang Cai","doi":"10.1007/s00604-025-07535-w","DOIUrl":"10.1007/s00604-025-07535-w","url":null,"abstract":"<div><p>DNA walker–based strategies are confronted with significant challenges in harmonizing design complexity, sequence dependence, and amplification efficiency. This study describes the innovative design of a double-stranded DNA probe, named the “LW probe,” which integrates a locked DNAzyme segment, enabling the coupling of the entropy-driven amplification (EDA) process with a DNAzyme-powered DNA walker. In the absence of the target, the “LW probe” remains in an inactive (“OFF”) state. Upon encountering target rotavirus sequences, the LW probe receives the trans-cleavage activity of Cas13a/crRNA and undergoes a conformational change, transforming into an activated structure. This structural transition initiates the EDA process continuously, leading to the release of the DNAzyme segment. Subsequently, the released DNAzyme segment acts on the surface of gold nanoparticles (AuNPs), cleaving the “Substrate probe” and consequently liberating fluorescence signals. Distinct from traditional DNA walkers that rely exclusively on the EDA for product amplification, the proposed approach synergistically combines the high-precision target recognition capacity of the EDA process with the potent signal amplification efficiency of DNA walkers. This integration results in remarkable enhancements in both specificity, demonstrated by the ability to discriminate single-base mismatched sequences, and sensitivity, with a detection limit as low as 2.7 fM. By synergizing EDA with the DNAzyme-driven DNA walker, our method achieves high sensitivity, with a detection limit of 2.7 fM, outperforming or matching the performance of previous DNA walker-based systems. This system enables highly sensitive and specific detection of low-abundance rotavirus with robust stability, offering a promising platform for disease diagnosis and biomedical research.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A multi-thionine DNA network-driven electrochemical aptasensor with dual signal amplification for ultrasensitive interleukin-6 detection 一种多硫氨酸DNA网络驱动的双信号放大电化学配体传感器，用于超灵敏的白细胞介素-6检测

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-09-12 DOI: 10.1007/s00604-025-07322-7

Linru Bai, Benqi Chen, Yan Xu, Mingjia Liu, Yunhui Yang, Jianmei Yang, Rong Hu

{"title":"A multi-thionine DNA network-driven electrochemical aptasensor with dual signal amplification for ultrasensitive interleukin-6 detection","authors":"Linru Bai, Benqi Chen, Yan Xu, Mingjia Liu, Yunhui Yang, Jianmei Yang, Rong Hu","doi":"10.1007/s00604-025-07322-7","DOIUrl":"10.1007/s00604-025-07322-7","url":null,"abstract":"<div><p>Au NPs@FGO@Fe MOG were sythesized for its inaugural application in biosensor development and engineered as a novel, highly efficient electrochemical aptasensor for the precise detection of interleukin-6 (IL-6). The aptasensor architecture integrates Au NPs@FGO@Fe MOG as the electrode interface and an anemone-like PtNi nanoclusters@MIL-anchored three-dimensional DNA (PD) network as the signal transduction matrix. The as-prepared Au NPs@FGO@Fe MOG exhibited an exceptionally high specific surface area, excellent electrical conductivity, and robust structural stability, collectively contributing to a pronounced enhancement in the performance of an electrochemical biosensor. The hierarchically PD network provided abundant binding sites for the redox-active probe thionine (Thi), thereby amplifying the electrochemical signal with remarkable efficiency. Under optimized conditions, the aptasensor achieved an impressive detection limit of 0.89 pg/mL. Moreover, the platform demonstrated reliable performance in detecting IL-6 in unspiked human serum samples, with results exhibiting high concordance with those obtained from commercial enzyme-linked immunosorbent assay (ELISA) kits. These findings demonstrate the considerable potential of this platform for rapid, sensitive, and accurate cytokine quantification, offering valuable prospects for clinical diagnostic applications.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon dot (CD)-based fluorescent probes for rapid and real-time tracking lysosomes movement and ATP monitoring in live cell 基于碳点（CD）的荧光探针用于快速实时跟踪溶酶体运动和监测活细胞中的ATP

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-09-11 DOI: 10.1007/s00604-025-07537-8

Chuyao Ni, Zihao Zhou, Jun Cao, Jiaqi Pan, Chaorong Li, Yingying Zheng

{"title":"Carbon dot (CD)-based fluorescent probes for rapid and real-time tracking lysosomes movement and ATP monitoring in live cell","authors":"Chuyao Ni, Zihao Zhou, Jun Cao, Jiaqi Pan, Chaorong Li, Yingying Zheng","doi":"10.1007/s00604-025-07537-8","DOIUrl":"10.1007/s00604-025-07537-8","url":null,"abstract":"<div><p>The detection of lysosomal dysfunction and abnormal adenosine triphosphate (ATP) levels is crucial due to their roles in various cellular disorders and tumor progression, respectively. In this paper, carbon dot (CD)-based fluorescent probes (CDs-1 and CDs-2) with rapid lysosomal targeting and real-time lysosomal monitoring capability were designed. Through a hydrothermal method, the different nitrogen doping contents of CDs-1 and CDs-2 were controlled by varying the ethylenediamine content while the lysosome-targeting ability derived from their neutral red precursor was preserved. CDs-1 and CDs-2 rapid and effectively targeted lysosomes in both H1975 and 4T1 cells, with Pearson’s colocalization coefficients of 0.94 and 0.88 (CDs-1) and 0.84 and 0.84 (CDs-2), closely matching the staining patterns of commercial lysosomal markers. CDs-1 demonstrated high specificity and sensitivity for ATP detection, displaying a linear detection range of 4–44 μM with a detection limit as low as 2.86 μM. CDs-1 enables monitoring of intracellular ATP level changes in the live cells under etoposide stimulation, while CDs-2 exhibited outstanding photostability and enabled real-time and long-term tracking of lysosomal movement. This study establishes CD-based fluorescent probes as valuable tools for investigating lysosomal function, with potential applications in cell biology research, disease mechanism studies, and drug screening platforms.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced electrochemiluminescence aptasensor using ZnO as the co-reaction accelerator of Mn-NHCDs/TEA system for the detection of florfenicol 用ZnO作为Mn-NHCDs/TEA体系共反应促进剂的增强型电化学发光感应传感器检测氟苯尼考

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-09-11 DOI: 10.1007/s00604-025-07490-6

Shan Ou, Min Qing, Zhaode Mu, Yonghua Yuan, Yueyuan Li, Lijuan Bai

{"title":"Enhanced electrochemiluminescence aptasensor using ZnO as the co-reaction accelerator of Mn-NHCDs/TEA system for the detection of florfenicol","authors":"Shan Ou, Min Qing, Zhaode Mu, Yonghua Yuan, Yueyuan Li, Lijuan Bai","doi":"10.1007/s00604-025-07490-6","DOIUrl":"10.1007/s00604-025-07490-6","url":null,"abstract":"<div><p> A novel ternary electrochemiluminescence (ECL) aptasensor was creatively proposed for florfenicol (FF) detection, on account of the manganese-nitrogen dual-doped carbon dots (Mn-NHCDs) emitter and the facilitation of triethylamine (TEA) by zinc oxide (ZnO). First, Mn-NHCDs with low excitation potentials and excellent electrochemical luminescence properties were synthesized. Then, a newly formulated ZnO with an enlarged specific surface area and an appropriate mesoporous structure was utilized to load Mn-NHCDs, thereby diminishing the leakage of Mn-NHCDs and stabilizing the luminescence within the system. More importantly, ZnO can serve as a new co-reaction accelerator for TEA to improve the reaction rate of Mn-NHCDs with TEA efficiently. Furthermore, owing to the overlap of spectra, we employed an ECL resonance energy transfer (ECL-RET) strategy, with the Mn-NHCDs/ZnO as the donor and black hole quencher (BHQ) as the acceptor. The ECL biosensor illustrates an extremely selective and sensitive determination of FF from 1.00 fg mL⁻<sup>1</sup> to 10.0 ng mL⁻<sup>1</sup> and a detection Limit of 0.413 fg mL⁻<sup>1</sup>. We envision that our aptasensor will provide substantial assurance concerning food safety.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

All-solid-state flexible visualization sensor based on sandwich structure and light-induced potential variation 基于夹心结构和光致电位变化的全固态柔性可视化传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-09-11 DOI: 10.1007/s00604-025-07503-4

Zheng Wang, Jiayuan Zhu, Ying-Zhuo Shen, Cheng Ma, Hongbo Li, Wei Liu, Xiao-Ya Hu, Qin Xu

{"title":"All-solid-state flexible visualization sensor based on sandwich structure and light-induced potential variation","authors":"Zheng Wang, Jiayuan Zhu, Ying-Zhuo Shen, Cheng Ma, Hongbo Li, Wei Liu, Xiao-Ya Hu, Qin Xu","doi":"10.1007/s00604-025-07503-4","DOIUrl":"10.1007/s00604-025-07503-4","url":null,"abstract":"<div><p>A novel all-solid-state flexible electrochromic sensor without liquid electrolytes and external power sources is reported which simplifies device construction and instrumentation requirement and offers high stability and energy efficiency. A polyacrylamide hydrogel was used as the solid electrolyte, and prussian blue (PB) on one end of a flexible bipolar electrode (BPE) served as the electrochromic region. Molecularly imprinted polymers (MIPs) modified on the other pole of the BPE, as electron injection regions, recognized targets and formed a sandwich structure with the aptamer functionalized light-responsive zirconium-based metal–organic frameworks (UiO-66) coated TiO<sub>2</sub> (Apt-UiO-66@TiO<sub>2</sub>) quantitatively. Under ultraviolet light-irradiation, the captured TiO<sub>2</sub>@UiO-66 triggered potential differences between the two poles of the BPE, causing the discoloration of PB. The degree of PB color change correlated with the concentration of bisphenol A (BPA) in the range 1.0 × 10<sup>–6</sup> to 1.0 × 10<sup>–12</sup> M. BPA could be quantified via the intelligent RGB analysis, and the limit of detection reaches 5.96 × 10<sup>–13</sup> M. The sensor has been applied to the assay of BPA in bottled water samples with satisfactory results. This all-solid-state flexible sensor can be integrated into flexible devices with enhanced portability for field applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145037413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrochemical aptasensor for hydrocortisone detection using V-PMOF-derived porous nanomaterials from V2CTx precursors 基于V2CTx前体的v - pmof衍生多孔纳米材料制备氢化可的松电化学感应传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-09-11 DOI: 10.1007/s00604-025-07404-6

Zhuo Shi, Shuyi Yang, Zhenxia Ma, Zifeng Wang, Zhanhong Li, Linlin Wang, Ľubomír Švorc, Zhigang Zhu

{"title":"An electrochemical aptasensor for hydrocortisone detection using V-PMOF-derived porous nanomaterials from V2CTx precursors","authors":"Zhuo Shi, Shuyi Yang, Zhenxia Ma, Zifeng Wang, Zhanhong Li, Linlin Wang, Ľubomír Švorc, Zhigang Zhu","doi":"10.1007/s00604-025-07404-6","DOIUrl":"10.1007/s00604-025-07404-6","url":null,"abstract":"<p>An electrochemical aptasensor for hydrocortisone (HC) detection was developed using a vanadium-based porphyrinic metal–organic framework (V-PMOF) materials synthesized from vanadium-based MXene (V₂CTₓ) and tetrakis (4-carboxyphenyl) porphyrin (TCPP), followed by thermal treatment at 800 °C to obtain V-PMOF<sub>800°C</sub>. The resulting V-PMOF<sub>800°C</sub> retained a nanoflower-like porous structure, enhancing electron transport, structural integrity, and electroactive site accessibility. Gold nanoparticles (Au NPs) were electrodeposited onto the V-PMOF<sub>800°C</sub>-modified electrode to improve conductivity and enable thiolated DNA aptamer (DNA<sub>apt</sub>) immobilization via Au–S bonding. Electrochemical measurements, particularly differential pulse voltammetry (DPV), were employed to evaluate the performance of the aptasensor, which exhibited a wide linear concentration range (0.05 nM–5 μM) and a low detection limit (LOD) of 10 pM. This work introduces a promising strategy for constructing MXene or MOF-based aptasensors with high sensitivity, selectivity, and stability for practical hormone monitoring applications.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0