Microchimica Acta最新文献

{"title":"Freeze-thaw-directed interfacial assembly of a gold nanoparticle/MXene electrochemical aptamer sensor for antibody-free myeloperoxidase detection","authors":"Congyu Min,&nbsp;Haifeng Sun,&nbsp;Haixia Zhang,&nbsp;Jinzhu Zhang,&nbsp;Min Ding,&nbsp;Xiaoqing Zhang","doi":"10.1007/s00604-026-08406-8","DOIUrl":"10.1007/s00604-026-08406-8","url":null,"abstract":"<div><p>An electrochemical aptamer-based (E-AB) sensor for myeloperoxidase (MPO) was developed that combined a gold nanoparticle /MXene scaffold with freeze-thaw-directed aptamer immobilization and probe-density optimization. Unlike using nanocomposite amplification alone, this strategy regulates probe density and accessibility at the bioreceptor layer to improve target-coupled signal transduction. Using the same aptamer solution, the freeze-thaw method yielded 60.77% greater MPO-induced signal suppression and a 2.4-fold lower regression-derived limit of detection (LOD) than conventional incubation. After target incubation for 50 min at 4 °C, the sensor showed a linear response from 5.00 to 250.0 ng/mL in 1% plasma matrix (equivalent to 500 ~ 25,000 ng/mL in undiluted plasma), with an LOD of 4.15 ng/mL (equivalent to 415 ng/mL in undiluted plasma). These results illustrated that coupling conductive nanointerface design with aptamer-layer organization might provide a practical route for antibody-free and H₂O₂-independent MPO determination.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148986915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An interface-regulated colorimetric-electrochemical dual-mode aptasensor for acetamiprid detection","authors":"Jiayu Liang,&nbsp;Zihan Feng,&nbsp;Guannan Dong,&nbsp;Liting Chai,&nbsp;Yanru Wang,&nbsp;Jianlong Wang,&nbsp;Min Ma","doi":"10.1007/s00604-026-08399-4","DOIUrl":"10.1007/s00604-026-08399-4","url":null,"abstract":"<div><p>Matrix-derived electroactive interference remains a major analytical challenge for electrochemical aptasensors in complex food samples. We develop a sequential dual-mode aptasensor based on interface regulation for reliable colorimetric screening and electrochemical quantification of acetamiprid. The aptasensor is constructed on a g‑C₃N₄/AuNP-modified electrode by immobilizing complementary DNA (cDNA), which hybridizes with AuPt@FeₓO_γ nanozyme-labeled aptamer probes. Upon acetamiprid introduction, target-induced competitive binding drives partial dissociation of the nanozyme probes from the electrode surface. A subsequent PBS rinsing step enables controlled regulation of the sensing interface by removing dissociated probes and coexisting interferents. The released probes are collected for colorimetric screening based on nanozyme-catalyzed TMB oxidation over a concentration range from 10.0 pg mL⁻¹ to 1.0 µg mL⁻¹, followed by electrochemical quantification using the washed electrode over a range from 10.0 fg mL⁻¹ to 1.0 ng mL⁻¹. The limits of detection are 0.073 pg mL⁻¹ for the colorimetric mode and 0.495 fg mL⁻¹ for the electrochemical mode. In this sequential workflow, colorimetric screening offers rapid visual feedback while electrochemical quantification provides sensitive trace-level analysis; the two modes enable mutual signal validation, reducing false-positive and false-negative responses and improving analytical reliability. Satisfactory accuracy and reproducibility are achieved in real food samples, demonstrating that interface-mediated matrix decoupling is an effective strategy for robust aptasensing in complex matrices.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148986797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient fluorescence strategy based on a DNA tetrahedral nanodevice for intracellular hypochlorous acid imaging","authors":"Yangfan Chen,&nbsp;Xi Zhou,&nbsp;Hailun He,&nbsp;Dejian Yuan,&nbsp;Changbei Ma","doi":"10.1007/s00604-026-08409-5","DOIUrl":"10.1007/s00604-026-08409-5","url":null,"abstract":"<div><p>Hypochlorous acid (HClO) is a pivotal effector molecule in the immune defense system of living organisms and plays a critical role in maintaining physiological homeostasis. Aberrant HClO levels are reported to be associated with numerous diseases, making it a promising disease biomarker in clinical settings. In this study, an efficient fluorescence detection strategy based on a DNA tetrahedron was constructed, for specific HClO imaging in living cells. This method enables specific recognition of HClO through a phosphorothioate (PS) modification site. Under optimized conditions, this method showed a detection range of 0.05-5 µM, with a limit of detection of 31.5 nM, enabling highly sensitive and specific detection of HClO. Recovery tests futher confirm its stable and accurate detection performance in complex samples. Moreover, this method was successfully applied to HClO imaging in RAW 264.7 cells, demonstrating satisfactory results. Overall, the method proposed here has many advantages, including high sensitivity, excellent stability, strong resistance to interference, simple detection process, and fast response, offering it considerable potential for biochemical analysis and disease diagnosis.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148986758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An advanced carbon/bismuth oxide nanoparticle-based filament for multifunctional 3D-printed electrochemical sensors toward paracetamol and lead in pharmaceutical, clinical, and environmental electroanalysis","authors":"Natália Marinho Caldas,&nbsp;Amanda Garcia Batista,&nbsp;Daniel M.-D. L. Alves,&nbsp;Samuel C. Silva,&nbsp;Diego A. Peixoto,&nbsp;Ariadne C. Catto,&nbsp;Valmor R. Mastelaro,&nbsp;Edson Nossol,&nbsp;Lucas V. de Faria,&nbsp;Diego P. Rocha,&nbsp;Bruno Campos Janegitz,&nbsp;Rafael Machado Dornellas","doi":"10.1007/s00604-026-08401-z","DOIUrl":"10.1007/s00604-026-08401-z","url":null,"abstract":"<div><p>3D printing offers a powerful, scalable approach to fabricating low-cost, high-performance electrochemical sensors for pharmaceutical and bioanalytical applications. We report the development of a 3D-printed graphite/polylactic acid (G/PLA) conductive composite electrode modified with bismuth oxide nanoparticles (Bi₂O₃NPs) to enhance paracetamol (PCT) detection. The optimized composition (G/Bi₂O₃NPs/PLA, 30:12.5:57.5% <i>wt.</i>) was processed by 3D printing to obtain electrodes without the need for complex post-treatment or electrochemical activation. Electrochemical impedance spectroscopy (EIS) revealed a marked decrease in the charge-transfer resistance (Rct) after Bi₂O₃NPs incorporation, indicating improved interfacial electron transfer. Electrochemical characterization demonstrated a significant increase in the PCT oxidation current and a shift in the peak potential toward less positive values, indicating the electrocatalytic role of Bi₂O₃NPs. Under optimized batch injection analysis with amperometric detection (BIA-AD) conditions, the sensor exhibited a wide linear dynamic range of 0.05–300 µmol L⁻¹, a low limit of detection of 0.006 µmol L⁻¹, and precision (RSD &lt; 5%). Inter-electrode reproducibility was satisfactory (RSD &lt; 5%), highlighting the robustness of the fabrication process. The proposed platform also demonstrated applicability to Pb(II) detection, evidencing the versatility of the G/Bi₂O₃NPs/PLA composite and expanding its potential for environmental monitoring and heavy metal analysis. Furthermore, selectivity studies conducted in the presence of common interferents showed negligible changes in the PCT response, confirming the sensor’s reliability for pharmaceutical quality control applications. Overall, the 3D-printed G/Bi₂O₃NPs/PLA electrode combines analytical performance with simplicity of fabrication, low cost, and scalability, positioning it as a promising alternative to conventional electrochemical sensors for routine PCT determination.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13574912/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148965978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly sensitive electrochemical immunosensor for alpha-fetoprotein detection based on trimetallic metal-organic framework nanozymes with peroxidase-like activity","authors":"Xuanming Xu,&nbsp;Yongqi Li,&nbsp;Peiru Nie,&nbsp;Yutong Kang,&nbsp;Lian Tang,&nbsp;Xin Zhou,&nbsp;Fengzhou Yang,&nbsp;Yihe Zhang,&nbsp;Yajie Wang","doi":"10.1007/s00604-026-08403-x","DOIUrl":"10.1007/s00604-026-08403-x","url":null,"abstract":"<div><p>Quantitative analysis of alpha-fetoprotein (AFP) in biological samples is essential for early diagnosis, accurate monitoring, and assessment of therapeutic efficacy. We developed a sandwich-type electrochemical biosensor based on trimetallic nanozyme for AFP sensitive detection. The UiO-66-NH₂@Au/AgNPs nanozyme was assembled via 11-mercaptoundecanoic acid (MUA) modification, and displayed exceptional peroxidase-like activity, due to its dense catalytic sites and synergistic multimetal effects. Besides, steady-state kinetic experiments were further performed to elucidate the structure-activity relationships of the trimetallic nanozyme. To enhance detection sensitivity, a MoS₂/Ni₃(HITP)₂ composite was employed as the substrate, leveraging its superior electrical conductivity to significantly amplify the biosensor signal. As a result, by determinating the nanozyme-catalyzed current response toward H₂O₂, the electrochemical platform achieved a wide linear range of 0.075–1000 ng·mL^− 1, with a limit of detection (LOD) of 0.05 ng·mL^− 1. The practical utility of this method was validated via spiked recovery tests in real samples, yielding an acceptable recovery of 90.98%–109.20%, and relative standard deviations (RSD) below 14.68%. Overall, this sandwich-type strategy combines the rapid response of electrochemical detection with the high specificity of antigen–antibody recognition. Although further optimization and validation are warranted for broader practical applications, the proposed platform demonstrates considerable potential for point-of-care testing and clinical cancer diagnosis.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148962866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitive and selective electrochemical detection of hydrocortisone using MoS2-SiO2 hybrid nanostructure modified glassy carbon electrode","authors":"Kunal,&nbsp;Shivram Jha,&nbsp;Prakash Chandra Singh,&nbsp;Deepak Kumar,&nbsp;Priyanka Yadav,&nbsp; Jitender,&nbsp;Pooja Sharma,&nbsp;Rajender S. Varma,&nbsp;Jamilur R. Ansari","doi":"10.1007/s00604-026-08410-y","DOIUrl":"10.1007/s00604-026-08410-y","url":null,"abstract":"<div><p>Mesoporous silica (m-SiO₂) has been synthesized from rice husk ash (RHA) and was deployed as a template to grow MoS₂. The resulting MoS₂-SiO₂ hybrid nanostructures were subsequently utilized for the immobilization and signal amplification of the hydrocortisone (HC) on a glassy carbon electrode (GCE). Transmission electron microscopy (TEM) images revealed the successful formation of MoS₂-SiO₂ hybrid nanostructures consisting of thin MoS₂ nanosheets decorated with amorphous SiO₂ domains. Nitrogen adsorption-desorption studies revealed a 534 m²/g multipoint BET surface area of hybrid and well-defined interconnected channels. Moreover, Small-angle X-ray diffraction (SAXRD) revealed that m-SiO₂ possessed a disordered structure. Prior to the formation of the hybrid nanostructure, silica was amine-functionalized using APTES. X-ray photoelectron spectroscopy (XPS) confirmed the functionalization of silica and the formation of MoS₂-SiO₂ hybrid nanostructures. The electrochemical properties of the hybrid nanostructure were studied using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), anodic stripping differential pulse voltammetry (ASDPV), and amperometry. EIS and CV studies of the MoS₂-SiO₂ hybrid nanostructure-modified glassy carbon electrode (MoS₂-SiO₂/GCE) exhibited a lower charge transfer resistance (R_ct) as compared to m-SiO₂/GCE. The fabricated hybrid sensor was employed for the electrochemical detection of hydrocortisone (HC). The concentration of HC in healthy individuals typically ranges from 10 to 100 nM. The calibration plot obtained from ASDPV exhibited a linear relationship between the oxidation peak current and HC concentration ranging from 1 to 100 nM. The fabricated sensor exhibited a high sensitivity of 0.12 µA/nM and a low limit of detection (LOD) of 1 nM. Furthermore, Amperometry and ASDPV studies conveyed that minimal change in oxidation current of HC in the presence of common interfering molecules present in biofluid, confirming the suitability, excellent selectivity and anti-interference capability of the proposed sensor.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148965961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiplex quantification of osteomyelitis pathogens based on bead array","authors":"Hehui Long,&nbsp;Guojun Li,&nbsp;Jiajin Wei,&nbsp;Lingfeng Yan,&nbsp;Hua Wang,&nbsp;Liming Zhang,&nbsp;Zhuoxuan Lu","doi":"10.1007/s00604-026-08408-6","DOIUrl":"10.1007/s00604-026-08408-6","url":null,"abstract":"<div><p>Accurate diagnosis of osteomyelitis is hindered by the limitations of conventional culture‑based methods, which lack the capacity for rapid simultaneous identification and quantification of multiple pathogens. Mixed infections involving multiple pathogens often exacerbate clinical outcomes, necessitating rapid and multiplex diagnostic approaches. We developed a multiplex detection assay integrating asymmetric solid-liquid biphasic PCR with a bead array platform, designated ASL-PCR, for the simultaneous identification of five common osteomyelitis‑associated pathogens: <i>Staphylococcus aureus</i> (<i>S. aureus</i>), <i>Escherichia coli</i> (<i>E. coli</i>), <i>Streptococcus pneumoniae</i> (<i>S. pneumoniae</i>), <i>Aspergillus fumigatus</i> (<i>A. fumigatus</i>), and <i>Candida albicans</i> (<i>C. albicans</i>). In this method, asymmetric PCR (aPCR) in liquid phase generates single‑stranded amplicons that are captured in real time by primer‑conjugated beads and extended on the bead surface. Biotin‑dCTP is incorporated during amplification, and the resulting products are labeled with streptavidin-phycoerythrin (SA‑PE) and detected by flow cytometry. The assay exhibited a wide dynamic range (2.94 × 10⁶ copies/µL to 7.53 × 10⁸ copies/µL) and high sensitivity, with the limits of detection (LOD) for <i>S. pneumoniae</i>, <i>E. coli</i>, <i>S. aureus</i>, <i>C. albicans</i>, and <i>A. fumigatus</i> being 1.84 × 10⁶ copies/µL, 5.28 × 10⁶ copies/µL, 1.24 × 10⁷ copies/µL, 1.22 × 10⁷ copies/µL, and 1.47 × 10⁷ copies/µL, respectively—sufficient for detecting clinically relevant pathogen loads after amplification. Notably, the assay demonstrated excellent specificity, with cross‑reactivity between any two pathogens below 2.5%. Spike‑and‑recovery experiments in serum yielded recoveries of 88.67% to 108.56%, indicating that the assay maintains high quantification accuracy even in complex biological matrices. Comparison with quantitative real-time PCR (qPCR) demonstrated strong correlation. Collectively, these findings support the proof-of-concept analytical feasibility and preliminary technical performance of the ASL-PCR assay for simultaneous detection of osteomyelitis-associated pathogens.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148927433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient fabrication of paper-based microfluidic biosensors incorporating laser-induced graphene for label-free electrochemical immunoassays","authors":"Yuxin Pan,&nbsp;Jiayin Chi,&nbsp;Yuyang Gu,&nbsp;Erzhen Chen,&nbsp;Xiao Li,&nbsp;Huan Liu,&nbsp;Dichen Li","doi":"10.1007/s00604-026-08393-w","DOIUrl":"10.1007/s00604-026-08393-w","url":null,"abstract":"<div><p>To improve the performance in electrochemical immunoassays, paper-based devices typically necessitate the introduction of transducing materials through additional preparation steps, and the assay procedures often require labeling steps. Here we show that laser fabrication can generate both transducing and microfluidic components of paper-based devices to support label-free immunoassays. Laser parameters were adjusted to improve the electrochemical properties of paper-based graphenic transducing structures. Surface treatment for label-free biosensing was performed, and the device configuration and operation for electrochemical impedance spectroscopy sensing were explored. Interleukin-6 protein was used as a representative target to evaluate the analytical performance of the developed immunosensors. In phosphate buffered saline, the immunosensors exhibited a sensitivity of 67.95 Ω/decade and a limit of detection of 4.66 pg/mL. In artificial serum, the immunosensors exhibited a sensitivity of 57.08 Ω/decade and a limit of detection of 5.35 pg/mL. The clinical application potential was further demonstrated with human serum samples. The presented approach for developing paper-based microfluidic immunosensors can enhance their performance while maintaining ease of fabrication and usage to meet different requirements in clinical practice.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148927434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A molecularly imprinted electrochemical sensor for detection of bisphenol A based on dendritic Pt–Pd bimetallic nanoparticles and g-C3N4 nanocomposite","authors":"Siying Wu,&nbsp;Tianmei Du,&nbsp;Mengyun Ma,&nbsp;Fang Pu,&nbsp;Yanli Zhang,&nbsp;Xue Men,&nbsp;Hongbin Wang,&nbsp;Wenrong Yang,&nbsp;Pengfei Pang","doi":"10.1007/s00604-026-08400-0","DOIUrl":"10.1007/s00604-026-08400-0","url":null,"abstract":"<div><p>The development of a novel molecularly imprinted electrochemical sensor for the sensitive and selective detection of bisphenol A (BPA) is reported, based on a nanocomposite comprising dendritic Pt–Pd bimetallic nanoparticles and graphitic carbon nitride (Pt–Pd NPs/g-C₃N₄). The molecularly imprinted polymer (MIP) was fabricated via electrochemical polymerization on Pt–Pd NPs/g-C₃N₄ nanocomposite modified screen-printed electrode (Pt–Pd NPs/g-C₃N₄/SPE) surface. The synergistic integration of porous Pt–Pd NPs and ultrathin g-C₃N₄ nanosheets provides a larger effective surface and enhances catalytic activity for BPA determination. The MIP film was fabricated via cyclic voltammetry on the modified electrode, employing BPA as the template molecule and pyrrole as the functional monomer. Following template removal, the resulting molecularly imprinted cavities within the MIP demonstrated high specificity for BPA recognition. The fabricated MIP-based electrochemical sensor exhibited an excellent linear response and high sensitivity for BPA detection over a broad concentration range of 2–100 ng/mL, achieving a limit of detection (LOD) of 0.2 ng/mL (3<i>σ/S</i>) and a limit of quantification (LOQ) of 0.6 ng/mL. The MIP-based sensor exhibits outstanding specificity, reproducibility, and operational stability, thereby providing a versatile platform for rapid and ultrasensitive BPA detection—a critical capability for ensuring environmental safety and protecting public health.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148927432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chiral metal-organic cage core-shell composite UMOP-S-2- Ppa@SiO2 used as stationary phase for HPLC enantioseparation","authors":"Yi-Juan He,&nbsp;Yu-Lan Zhu,&nbsp;Yun-Qiao Zhao,&nbsp;Ren-Xiu He,&nbsp;Sheng-Ming Xie,&nbsp;Bang-Jin Wang,&nbsp;Jun-Hui Zhang,&nbsp;Li-Ming Yuan","doi":"10.1007/s00604-026-08397-6","DOIUrl":"10.1007/s00604-026-08397-6","url":null,"abstract":"<div><p> A facile one-pot strategy was developed to construct a core-shell UMOP-S-2-Ppa@SiO₂ composite using (S)-2-phenylpropionic acid as chiral selector. The prepared UMOP-S-2-Ppa@SiO₂ composite was utilized as a novel chiral stationary phase (CSP) for HPLC enantioseparation. Under normal-phase HPLC conditions, the packed column afforded effective enantioseparation for 18 racemates including alcohols, aldehydes, esters, ketones, amines, and epoxy compounds, among which 11 pairs of enantiomers achieved baseline resolution. Compared with the widely used Chiralpak AD-H and Chiralcel OD-H columns, the developed chiral column exhibited good chiral separation complementarity. It can efficiently resolve some racemates that fail to be separated by the above commercial columns. The column also demonstrated satisfactory repeatability and stability, and the relative standard deviations of retention time and peak area remained below 1.0% after 250 consecutive injections. These results demonstrate that chiral metal-organic cages(MOC)@SiO₂ core-shell composites prepared via a post-synthetic modification strategy hold great promise for HPLC enantioseparation, offering new insights into the development of MOC-based materials for chromatographic applications.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148927172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}