Microchimica Acta最新文献

{"title":"Carbon dot-graphene oxide-based luminescent nanosensor for creatinine detection in human urine","authors":"Poornima Bhatt,&nbsp;Deepak Kukkar,&nbsp;Ashok Kumar Yadav","doi":"10.1007/s00604-024-06838-8","DOIUrl":"10.1007/s00604-024-06838-8","url":null,"abstract":"<div><p>A fluorescence (FL)-based nanosensor has been devised for creatinine (CR) detection in human urine specimens. The proposed nanosensor utilized a nanocomposite (NC) of carbon dots (CDs) and graphene oxide (GO). The formation of CDs/GO NC reduced the CD FL emission (<i>λ</i>_excitation = 390 nm, <i>λ</i>_emission = 461 nm) by ~ 75%. With the introduction of CR to the NC, the CD emission intensity was reinstated by approximately 70%. The linear detection range for CR was 10⁻⁵ to 0.1 mg dL⁻¹ (<i>R</i>² = 0.998), with a limit of detection of 4.3 × 10⁻² mg dL⁻¹. Additionally, CDs/GO NC exhibited outstanding consistency and specificity in recognizing CR within urine specimens from both healthy individuals and patients suffering from chronic kidney disease (CKD). The Bland–Altman assessment (utilizing 25 human urine specimens) displayed remarkable consensus (<i>R</i>² = 0.995) among the FL approach and the benchmark Jaffe technique. This observation indicates the hands-on usefulness of the nanosensor for identifying CR in biological specimens. </p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel miniaturized potentiometric electrode based on carbon nanotubes and molecularly imprinted polymer for the determination of lidocaine","authors":"Saad S. M. Hassan,&nbsp;Mahmoud Abdelwahab Fathy","doi":"10.1007/s00604-024-06802-6","DOIUrl":"10.1007/s00604-024-06802-6","url":null,"abstract":"<div><p>A novel miniaturized, solid-contact potentiometric screen-printed electrode was developed for highly sensitive and selective determination of lidocaine anesthetic. The electrode integrated single-walled carbon nanotubes as a solid-contact material and a molecularly imprinted polymer as a recognition sensory material. The performance characteristics of the electrode were evaluated and optimized to display a Nernstian slope of 58.92 ± 0.98 mV/decade over a linear concentration range of 4.53 × 10⁻⁷ to 6.18 × 10⁻³ mol/l within &lt; 6 s. The detection limit was 7.75 × 10⁻⁸ mol/l (18.16 ng/ml) of lidocaine. The use of the molecularly imprinted polymer significantly enhanced the selectivity of the electrode, and carbon nanotubes increased the sensitivity, accuracy, and potential stability. The electrode was successfully used for determining lidocaine in pharmaceutical preparations and human urine. The results favorably compared with data obtained by liquid chromatography–tandem mass spectrometry.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06802-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of CuFe2O4 microspheres/carbon sheets composite materials as a sensitive electrochemical sensor for determination of bisphenol A","authors":"Ying Wang,&nbsp;Xinming Wang,&nbsp;Mingyue Chu,&nbsp;Jianjiao Xin,&nbsp;Zhongxin Jin,&nbsp;Huiyuan Ma,&nbsp;Kevin P. O’Halloran,&nbsp;Yingji Wang,&nbsp;Haijun Pang,&nbsp;Guixin Yang","doi":"10.1007/s00604-024-06806-2","DOIUrl":"10.1007/s00604-024-06806-2","url":null,"abstract":"<div><p>A composite material based on CuFe-ZIF-derived CuFe₂O₄ nano-microspheres grown <i>in situ</i> and well-ordered on carbon sheets (CS) was prepared and applied for highly effective determination of bisphenol A (BPA). The composite material possessed inherently high redox activity due to the presence of both Cu and Fe ions with various oxidation states (Cu²⁺/Cu⁺ and Fe³⁺/Fe²⁺), high specific surface area, uniform distribution of Cu and Fe ions, and a robust framework imparted by its precursor CuFe-ZIF. This led to increased active sites for electrochemical reactions, improved electron transfer efficiency, and structural integrity during electrochemical cycling. Furthermore, combining CS with CuFe₂O₄ not only provided a large surface area to support well-ordered CuFe₂O₄ nano-microspheres without aggregation, but also enhanced the conductivity and mechanical stability of the CuFe₂O₄/CS composite. This results in synergistic effects that enhanced the overall performance of the composite material. In addition, both copper and iron are relatively non-toxic and abundant, making CuFe₂O₄/CS safe and cost-effective for large-scale applications. Consequently, the CuFe₂O₄/CS-modified electrode shows highly efficient electrochemical sensing properties with a wider detection range of 0.009-168 µM and lower detection limit of 0.0027 µM (S/<i>N</i> = 3) compared with most reported BPA sensors. It also has an optimized current at pH 7 which is convenient for real world applications. This CuFe₂O₄/CS modified electrode as a highly sensitive electrochemical platform can be applied to monitor BPA concentrations in bottled water with good recovery (97.2-102.2%).</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conducting polymer functionalized Cu-metal organic framework–based electrochemical immunosensor for rapid and sensitive quantitation of Escherichia coli O157:H7","authors":"Xiaoge Duan,&nbsp;Xinrui Shi,&nbsp;Zhaoyuan He,&nbsp;Hongcai Chen,&nbsp;Zhuanzhuan Shi,&nbsp;Zhi Zhao,&nbsp;Hailan Chen,&nbsp;Meiling Yu,&nbsp;Chunxian Guo","doi":"10.1007/s00604-024-06807-1","DOIUrl":"10.1007/s00604-024-06807-1","url":null,"abstract":"<div><p><i>Escherichia</i> <i>coli</i> (<i>E. coli</i>) O157:H7 is an important food-borne pathogen that can cause hemorrhagic diarrhea and enteritis in humans and animals. Realizing the rapid quantitation of <i>E. coli</i> O157:H7 is of great significance for the guarantee of food safety and disease control. In this study, an electrochemical immunosensing technique based on a functionalized composite of Cu-metal organic framework (Cu-MOF) and poly (3,4-ethylenedioxythiophene)-poly (styrenesulfonate) (PEDOT:PSS) is developed, achieving rapid and sensitive quantitation of <i>E. coli</i> O157:H7 in food and clinical feces samples. The organic functionalization of Cu-MOF significantly improves the interface conductivity to facilitate electron transfer and provides the sulfonic groups (–SO₃H) to conjugate bio-recognizing elements for target determination. The immunosensor delivers a linear detection range of 3 × 10² ~ 3 × 10⁸ cfu/mL, a low limit of detection (LOD) of 7.4 cfu/mL, and a short analysis time of 40 min. In addition, it does not show any cross-reactivity with other common pathogens and exhibits high repeatability with relative standard deviations (RSDs) all lower than 2.09%, providing a promising approach for warranting  food safety and control of <i>E. coli</i> O157:H7 disease.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly sensitive detection of MMP-2 using an electrochemiluminescent biosensor enhanced by ladder-branch hybridization chain reaction","authors":"Yuanxun Gong,&nbsp;Jiayi Zhang,&nbsp;Yunzhan Xi,&nbsp;Dingxie Jian,&nbsp;Chenyi Zhuo,&nbsp;Qianli Tang,&nbsp;Kai Zhang,&nbsp;Xianjiu Liao","doi":"10.1007/s00604-024-06761-y","DOIUrl":"10.1007/s00604-024-06761-y","url":null,"abstract":"<div><p> An advanced electrochemiluminescence (ECL) biosensor was developed that integrates T7 RNA polymerase amplification, ladder-branch hybridization chain reaction (HCR), and the precise targeting capabilities of CRISPR/Cas13a technology. The novelty of this research lies in the unique combination of these three cutting-edge technologies, which has not been previously utilized together in biosensing platforms, enabling highly sensitive and specific detection of biomolecules with exceptional precision. This innovative biosensor addresses the critical need for sensitive and specific detection of matrix metalloproteinase-2 (MMP-2), a key biomarker in cancer diagnostics. Through meticulous optimization of amplification and reaction conditions, the biosensor demonstrated remarkable sensitivity and specificity, achieving a detection limit as low as 6.34 aM, surpassing existing methodologies. The biosensor also exhibited excellent stability and reproducibility across multiple scans and maintained consistent functionality over an extended period, highlighting its reliability for practical applications. The effectiveness of the biosensor was validated using real samples, demonstrating its capability to accurately quantify MMP-2 in complex biological matrices with high recovery and minimal interference. The integration of isothermal amplification and CRISPR/Cas13a within the ECL biosensor platform represents a significant advancement in molecular diagnostics, offering a powerful tool for real-time monitoring of MMP-2. This combination of technologies sets the platform apart from traditional methods, marking a significant step forward in biosensor innovation. This biosensor holds substantial promise for revolutionizing cancer diagnostics and facilitating personalized treatment strategies, ultimately aiming to improve patient outcomes in cancer care. Future research may explore further enhancements and applications of this biosensor in various clinical settings.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic sensor using pH gradient with hybrid magnetoliposomes containing laccase immobilized nanocrystals","authors":"Vanesa Román-Pizarro,&nbsp;Ángela Écija-Arenas,&nbsp;Cornelia A. Hermann,&nbsp;Thomas Hirsch,&nbsp;Juan Manuel Fernández-Romero","doi":"10.1007/s00604-024-06829-9","DOIUrl":"10.1007/s00604-024-06829-9","url":null,"abstract":"<div><p>A method using pH gradient to modify the phospholipid dissociation has been developed using hybrid magnetoliposomes with encapsulated enzyme laccase, which has been immobilized on hydrophilic magnetic nanocrystals (PAA-MNCs) retained in the reaction/detection zone of a microfluidic system. The hybrid magnetoliposomes act as micro containers, providing the safe transfer of the enzyme to the reaction/detection site, where it is retained, and preconcentration of the enzyme in this place occurs, which improves the system’s sensitivity. The use of pH change involves the lack of external molecules to release the encapsulated materials. The laccase-mediated redox oxidation of the fluorescent substrate 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) to form its non-fluorescent oxide form has been used as the indicator reaction. The method was applied to determine potential laccase inhibitors in beverages and fruits. The study of the inhibitory effect has focused on compounds that follow different mechanisms: cysteine, malic acid, and fumaric acid. The dynamic ranges of the calibration graphs of these compounds were between 0.05–100, 0.02–100, and 0.1–100 μmol L⁻¹, respectively. The detection limits have been established between 6 and 30 nmol L⁻¹, and the precision expressed as the percent of relative standard deviation (RSD%) was between 2.5 and 5.1%. The overall microfluidic system showed a sampling frequency of 8 h⁻¹. The method has been applied to determine fumaric acid as a laccase inhibitor in several juice samples (apple, pear, and grape), with recovery values between 85 and 101%. The results were comparable to those obtained using a LC reference method.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing a molecularly imprinted polymer-based electrochemical sensor for the sensitive and selective detection of the antimalarial chloroquine phosphate","authors":"Ensar Piskin,&nbsp;Ahmet Cetinkaya,&nbsp;Mehmet Altay Unal,&nbsp;Briza Pérez-López,&nbsp;Udara Bimendra Gunatilake,&nbsp;Saad Sene,&nbsp;Yannick Guari,&nbsp;Joulia Larionova,&nbsp;Eva Baldrich,&nbsp;Sibel A. Ozkan","doi":"10.1007/s00604-024-06820-4","DOIUrl":"10.1007/s00604-024-06820-4","url":null,"abstract":"<div><p>For the first time an electrochemical sensor based on nanomaterial-supported molecularly imprinted polymers (MIPs) is applied to the sensitive and specific determination of chloroquine phosphate (CHL). The sensor was produced using an electropolymerization (EP) approach, and it was formed on a glassy carbon electrode (GCE) using CHL as a template and 2-acrylamido-2-methyl-1-propane sulfonic acid (AMPS) and aniline (ANI) as functional monomers. Incorporating Prussian blue polyethyleneglycol-amine nanoparticles (PB@PEG-NH₂) in the MIP-based electrochemical sensor increased the active surface area and porosity. The developed CHL/AMPS-PANI/PB@PEG-NH₂/MIP-GCE sensor was characterized morphologically and electrochemically using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and scanning electron microscopy (SEM). The indirect measurement of CHL was accomplished in 5.0 mmol L⁻¹ [Fe(CN)₆]^−3/−4 solution using differential pulse voltammetry, displaying linear response between 1.75 × 10⁻¹² and 2.50 × 10⁻¹³ M, and limits of detection (LOD) and quantitation (LOQ) of 6.68 × 10⁻¹⁴ M and 2.23 × 10⁻¹³ M, respectively, in standard solutions. CHL recoveries in spiked serum and tablet form ranged from 99.13 to 101.51%, while the relative standard deviations (RSD%) were below 2.41% in both types of samples. In addition, the sensor’s excellent selectivity was successfully demonstrated in the presence of components with a chemical structure similar to CHL.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RuSiNPs@N,S-GQDs as self-enhanced anodic electrochemiluminescent immunobeacons for the highly sensitive quantitation of okadaic acid in shellfish","authors":"Silun Li,&nbsp;Jiawei Peng,&nbsp;Xueying Lin,&nbsp;Jing Chen,&nbsp;Yanfang Wu,&nbsp;Quansheng Chen,&nbsp;Xiaomei Chen","doi":"10.1007/s00604-024-06823-1","DOIUrl":"10.1007/s00604-024-06823-1","url":null,"abstract":"<div><p>A competitive electrochemiluminescence (ECL) immunosensor is proposed to accurately and rapidly assess okadaic acid (OA) levels in shellfish using a novel self-reinforced solid-state ECL marker, which is essential for ensuring seafood safety. Graphene quantum dots doped with nitrogen and sulfur (<i>N,S</i>-GQDs) were synthesized, for the first time, through the electrolysis of graphite in 3-(<i>N</i>-morpholine) propane sulfonic acid solution. Intriguingly, these <i>N,S</i>-GQDs exhibited exceptional co-reactant properties, significantly enhancing the anodic ECL performance of Ru(bpy)₃²⁺ in a phosphate-buffered saline solution. Following the functionalization of Ru(bpy)₃²⁺-doped silica nanoparticles (RuSiNPs) with poly(diallyldimethylammonium) chloride, we achieved a well-dispersed assembly of <i>N,S</i>-GQDs on the exterior of the RuSiNPs through electrostatic interactions. Importantly, the core–shell structure of RuSiNPs@<i>N,S</i>-GQDs efficiently encapsulated both the luminophore and co-reactant, thus improving the transfer rates of electrons, shorting interaction distances, and reducing energy loss during light emission. Leveraging this “bright” ECL beacon, the ECL immunosensor demonstrated remarkable analytical performance, yielding a low half maximal inhibitory concentration (IC₅₀) of 0.14 ng mL⁻¹, an extensive linear range spanning 0.003–40 ng mL⁻¹, and impressively low limit of detection of 0.001 ng mL⁻¹ for OA determination.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A long straight square microchannel in viscoelastic fluid for focusing submicron-sized particles and bacteria","authors":"Youngseo Cho,&nbsp;Min-Ho Lee,&nbsp;SangWook Lee,&nbsp;Younghak Cho","doi":"10.1007/s00604-024-06824-0","DOIUrl":"10.1007/s00604-024-06824-0","url":null,"abstract":"<div><p>A viscoelastic flow focusing device is presented that enables simple and robust focusing of submicron-sized particles in the channel center by optimizing operating conditions such as channel length, flow rate and poly(ethylene oxide) (PEO) concentration. Submicron-sized particles (up to 100 nm) can be easily focused to the channel center under viscoelastic fluid flow without any external force via a simply fabricated microchannel with a long channel length and a large square cross-section. The device was fabricated using a common soft lithography technique for the polydimethylsiloxane (PDMS) channel, which has a width of 50 μm, a height of 50 μm and a channel length of 27 cm. The extralong channel enabled submicron-sized particle focusing, even in a channel of a relatively large size with high flow rate, which can realize flow cytometric applications. The focusing performance was first demonstrated using submicron-sized polystyrene (PS) beads ranging from 870 nm to 50 nm and then using biological particles such as <i>E. coli</i> bacteria to demonstrate the biological feasibility of the device. The PS beads, which ranged in diameter from 870 nm to 100 nm, were focused to the center of the channel, achieving over 90% focusing efficiency for beads as small as 510 nm and 62% focusing efficiency for 100-nm beads. The device could also align a bacterial suspension in the center of the channel at flow rates up to 30 µL/min, demonstrating its biological importance. The ability of the developed device to align submicron-sized particles within a narrow flow stream in a highly robust manner is promising for various biological and clinical applications, such as distinguishing pathogenic bacteria and evaluating individual antibiotic responses in a single experiment.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel electrochemiluminescent cytosensor using dual-target magnetic probe recognition and nanozymes-catalyzed cascade signal amplification for precise phenotypic enumeration of CTCs","authors":"Congcong Shen,&nbsp;Simin Fan,&nbsp;Xiaoqing Li,&nbsp;Fanshu Guo,&nbsp;Junru Li,&nbsp;Minghui Yang","doi":"10.1007/s00604-024-06825-z","DOIUrl":"10.1007/s00604-024-06825-z","url":null,"abstract":"<div><p>The inability of surgical biopsy to monitor the dynamic evolution of cancer cells hampers its capacity to reflect real-time tumor heterogeneity. Circulating tumor cells (CTCs), as a crucial target in liquid biopsy, offer a novel approach for accurate monitoring of tumors. However, the rarity and complex phenotype resulting from epithelial-mesenchymal transition pose challenges for conventional methods such as CellSearch and immunohistochemistry, which have insufficient ability for simultaneous phenotyping and enumeration of CTCs. The enumeration of a single phenotype CTCs is insufficient for accurately assessing disease progression. Herein, we propose a strategy to address this issue by fabricating an electrochemiluminescence cytosensor via the integration of dual-target enrichment and nanozymes-catalyzed cascade signal amplification. The graphene oxide@hollow mesoporous Prussian blue/Pt (GO@HMPB/Pt) complex, possessing a large specific surface area and exceptional catalytic activity, is employed for loading a substantial amount of luminol as the signal probe. Dual-target magnetic PPy@Fe₃O₄/Au-antibody/aptamer is utilized for the magnetic capture of both epithelial and interstitial CTCs. Glutathione (GSH) can disrupt the Au–S bond on aptamer by a thiol exchange reaction and selectively releases a specific subset of phenotypic CTCs, thereby facilitating the efficient capture, accurate classification, and ultrasensitive detection of CTCs in peripheral blood. Using the epithelial MCF-7 and mesenchymal Hela cells as models, the ECL cytosensor demonstrates excellent performance in identifying cells spiked into whole blood. This study presents a novel approach for early detection of metastasis, tracking tumor recurrence, and monitoring therapeutic efficacy.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}