Microchimica Acta最新文献

{"title":"Multiplexed immunochromatographic strip based on multicolor fluorescent microspheres for simultaneous detection of three herbicides","authors":"Hui Cao,&nbsp;Jie Chen,&nbsp;Li Zhao,&nbsp;Chenxu Yan,&nbsp;Lin Ma,&nbsp;Jinsong Yu,&nbsp;Xiuxiu Wu,&nbsp;Fei Xu","doi":"10.1007/s00604-026-08045-z","DOIUrl":"10.1007/s00604-026-08045-z","url":null,"abstract":"<div><p>A novel multiplex immunochromatographic assay (ICA) based on quantum-dot-encoded multicolor fluorescent microsphere (MFMs) was developed for the simultaneous and quantitative detection of prometryn (PRO), diquat (DQ), and sulfometuron-methyl (SMR). Red, yellow, and green MFMs were fabricated by embedding CdSe/ZnS QDs into polymer microspheres and subsequently conjugated with specific monoclonal antibodies, generating highly uniform MFM-mAb probes with clear and stable emission signals. The multiplex ICA exhibited excellent analytical performance, with limits of detection of 0.41 ng/mL for PRO, 9.36 ng/mL for DQ, and 1.32 ng/mL for SMR, better than most ELISA-based assays. The strip also demonstrated high specificity, good storage stability, and reliable performance in complex matrices. In spiked river water and vegetable samples, recoveries ranged from 85.53% to 109.67% with RSDs of 1.33–7.02%, indicating strong applicability. Overall, this MFM-ICA provides an effective platform for rapid multi-herbicide screening.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic TiO₂-doped g-C₃N₄@PoPD nanohybrid: toward efficient biosensing of xanthine","authors":"Deeksha Thakur,&nbsp;Chandra Mouli Pandey,&nbsp;Devendra Kumar","doi":"10.1007/s00604-026-08099-z","DOIUrl":"10.1007/s00604-026-08099-z","url":null,"abstract":"<div><p>An enzymatic biosensor based on poly-ortho-phenylenediamine (PoPD) coated with graphitic carbon nitride (g-C₃N₄) and titanium dioxide (TiO₂) was developed for the detection of xanthine (Xn). Microscopic and spectroscopic analyses confirmed the successful incorporation of TiO₂ into the g-C₃N₄@PoPD nanohybrid. The resulting nanocomposite exhibited excellent sensing performance, achieving a wide linear detection range from 1 pM to 1 µM and an ultralow detection limit of 0.2 pM. The biosensor demonstrated good stability for up to 5 weeks, high reproducibility, and strong selectivity toward Xn even in the presence of potential interfering analytes. Validation using real samples from Rohu (<i>Labeo rohita</i>) fish further confirmed its potential applicability for xanthine detection in meat freshness assessment.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exonuclease I-driven target recycling strategy integrated with PEI-CoSe2/PtPd NPs for ultrasensitive electrochemical detection of zearalenone","authors":"Gengan Du,&nbsp;Han Yan,&nbsp;Weidi Kong,&nbsp;Long Wang,&nbsp;Baoshan He,&nbsp;Qi Guo","doi":"10.1007/s00604-026-08109-0","DOIUrl":"10.1007/s00604-026-08109-0","url":null,"abstract":"<div><p>The widespread contamination of cereal crops by zearalenone (ZEN) poses a threat to food safety, demanding sensitive and reliable analytical methods. In this study, a signal-on electrochemical aptasensor was developed based on a dual-amplification strategy combining a PEI-CoSe₂/PtPd NPs nanocomposite with Exonuclease I (Exo I)-assisted target recycling. The PEI-CoSe₂/PtPd NPs nanocomposite provided enhanced electrode conductivity and a large surface area for efficient immobilization of DNA probes. In the presence of ZEN, the aptamer dissociated from its complementary strand, triggering Exo I-mediated cleavage and enabling target recycling, which led to signal amplification. Under optimized conditions, the aptasensor exhibited a linear response over the range 0.001–100 ng/mL with a detection limit of 0.18 pg/mL. In addition, the proposed sensor also showed good selectivity, reproducibility, and applicability in spiked corn samples. These results indicate that the developed aptasensor provides a feasible approach for ZEN detection in food samples.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GO-SELEX of aptamer for colorimetric aptasensor based on AuNPs@CeO₂ nanozyme in detection of gentamicin residues in milk.","authors":"Fengling Yue, Miaomiao Sui, Jian Liu, Bo Wang, Lijun Jiang, Yifan Du, Jiayi Ye, Yingying Nie, Guihong Zhao","doi":"10.1007/s00604-026-08110-7","DOIUrl":"https://doi.org/10.1007/s00604-026-08110-7","url":null,"abstract":"<p><p>Aptamer-based detection methods have emerged as a promising research direction for detecting gentamicin (GEN). Herein, the aptamer for GEN with K_d of 4.8 µM was firstly selected by non-immobilized graphene oxide (GO)-SELEX. With the molecular docking simulations and truncation strategies, the binding affinity of the truncated aptamer GEN-T-1 (53 nt) with K_d of 0.4 µM was further improved. Based on this, a colorimetric aptasensor for GEN detection was developed with GEN-T-1 as the recognition element and AuNPs@CeO₂ as the nanozyme which could catalyze oxidation of 3,3',5,5'-tetramethylbenzidine (TMB). In presence of GEN, the aptamer selectively bound to GEN, dissociating from nanozyme surface and generating a lighter color. The aptasensor demonstrated a low limit of detection 3.56 ng/mL and a wide linear detection range of 10-10,000 ng/mL, with excellent specificity, stability, repeatability, and reproducibility. The method was successfully applied to detect GEN in milk with recoveries of 97.97%-100.49%. The research results provide a theoretical foundation for the rapid and accurate detection of GEN.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147863324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation and application of a novel dendrimer modified silica reversed phase/hydrophilic interaction mixed-mode stationary phase","authors":"Jie Wen,&nbsp;Yanyu Jiang,&nbsp;Lian Zhong,&nbsp;Wuji Shuoti,&nbsp;Ruihan Peng,&nbsp;Xuemei Wang,&nbsp;Yaxin Yang,&nbsp;Jing Ou,&nbsp;Meifang Liao,&nbsp;Lujun Wang","doi":"10.1007/s00604-026-08103-6","DOIUrl":"10.1007/s00604-026-08103-6","url":null,"abstract":"<div><p>A novel dendritic mixed-mode stationary phase (Sil-BD-AFT) with the properties of reversed phase and hydrophilic interaction modes was designed and synthesized. The stationary phase and its intermediates were characterized by Fourier Transform Infrared Spectroscopy (FT-IR), Thermogravimetric Analysis (TGA), and Elemental Analysis (EA). The characterization results showed that the dendritic polymers were successfully grafted onto the silica particles. The performance of the stationary phase was evaluated using various analytes, such as alkylbenzenes, polycyclic aromatic hydrocarbons (PAHs), positional isomers, nucleosides, alkaloids, halogenated benzenes, anilines, and phenols. The stationary phase exhibited outstanding separation efficiency for these compounds under the separation modes of reversed phase liquid chromatography (RPLC) and hydrophilic interaction liquid chromatography (HILIC). Furthermore, a comparison with commercial columns demonstrated that Sil-BD-AFT, with its mixed-mode separation capability, provided superior separation performance for structurally complex analytes such as anilines, phenols, and alkaloids. The effects of mobile phase composition and column temperature to retention time were examined. Investigation of the retention mechanism was carried out using the Van’t Hoff equation and the Abraham Solvation Parameter Model, which revealed that <i>π-π</i> interaction, dipole-dipole interaction, hydrogen bonding, and hydrophobicity are the primary modes for chromatographic separation. The Van Deemter equation was employed to investigate the impact of flow rate to column efficiency. The prepared column exhibited excellent repeatability through the evaluation of chromatographic behaviors of multiple consecutive injections. Furthermore, the prepared chromatographic column can be further applied to detect bisphenol A in bills and phthalate esters in plastic wrap, respectively. The results open new avenues for the chromatographic separation of diverse analytes using dendrimers based mixed-mode stationary phases.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147830003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct/enrichment dual-mode lateral flow immunoassay enabled by a composite magnetic-fluorescent nanolabel for ultrasensitive detection of Helicobacter pylori","authors":"Tingting Huang,&nbsp;Ting Wu,&nbsp;Xuesong Xiong,&nbsp;Xiao Zhao,&nbsp;Jiahuan Zhang,&nbsp;Chongwen Wang,&nbsp;Bing Gu,&nbsp;Jiansong Gu,&nbsp;Fen Li","doi":"10.1007/s00604-026-08014-6","DOIUrl":"10.1007/s00604-026-08014-6","url":null,"abstract":"<div><p>Rapid, sensitive, and low-cost in vitro screening is vital for controlling <i>Helicobacter pylori</i> (<i>H. pylori</i>) infection and guiding eradication, yet remains challenging. Here we report a high sensitivity lateral flow immunoassay (LFIA) based on a multifunctional composite magnetic-fluorescent probe (MTQD-Ab) that enables accurate detection of <i>H. pylori</i> in stool with minimal pretreatment and supports two modes—rapid screening and precise quantification—tailored to different use scenarios. The probe features a core–shell–biorecognition architecture: a 200 nm Fe₃O₄ core for strong magnetic responsiveness, multilayer carboxylated quantum dots (QDs) for intense fluorescence, and specific antibodies on the outermost QD surface for rapid capture of <i>H. pylori</i>, providing dual signal amplification via magnetic enrichment and fluorescence enhancement. By functionally switching the MTQD-Ab nanolabel, the platform provides two modes: in the direct mode, sample dilution achieves a limit of detection (LOD) of 100 cells/mL within 15 min; in the enrichment mode, magnetic concentration of a 1 mL sample lowers the LOD to 10 cells/mL and eliminates potential hook effects, enabling accurate quantitation. Validation with 40 clinical stool samples demonstrated reliable accuracy and near PCR sensitivity while shortening total time from several hours to ~ 30 min and reducing cost, indicating strong potential for clinical application.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pendimethalin imprinted electrochemical sensor based on CuO-Bi2MoO6 nanocomposite and pendimethalin detection in real samples","authors":"Mustafa Anıl Erbağcı,&nbsp;Bahar Bankoğlu Yola,&nbsp;Neslihan Özdemir,&nbsp;Mehmet Lütfi Yola","doi":"10.1007/s00604-026-08095-3","DOIUrl":"10.1007/s00604-026-08095-3","url":null,"abstract":"<div><p>A novel approach is introduced for detection of pendimethalin (PEN) - one of the most widely utilized herbicides-  based on the synergistic properties of molecularly imprinted polymers (MIPs) and a CuO-Bi₂MoO₆ (Cu-Bi-Mo) nanocomposite. The methodology combined the specific recognition capabilities of MIPs with the enhanced sensing performance provided by the advanced nanocomposite material. The synthesis of the Cu-Bi-Mo nanocomposite was initiated through the application of the sol-gel method. After the glassy carbon electrode’s modification with the Cu-Bi-Mo nanocomposite, PEN imprinted electrodes were fabricated using cyclic voltammetry (CV) with a dispersion containing 100.0 mM pyrrole (Py) monomer and 25.0 mM PEN molecule. The electrochemical sensor revealed a detection range of 1.0 × 10^− 9 M to 1.0 × 10^− 8 M PEN and achieved a detection limit (LOD) of 3.30 × 10^− 10 M. To demonstrate its practical applicability, the electrochemical sensor was applied to drinking water and orange juice samples, yielding recovery results near 100%. This high recovery indicated the exceptional precision and reliability of the proposed electrochemical sensor. The electrochemical sensor’s performance was thoroughly assessed across several key metrics including selectivity, stability, and reproducibility.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-026-08095-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Photonic and nanomaterial-driven optical biosensing strategies for Parkinson’s disease","authors":"Divya Soni,&nbsp;Pooja Ratre","doi":"10.1007/s00604-026-08068-6","DOIUrl":"10.1007/s00604-026-08068-6","url":null,"abstract":"<div><p>Parkinson’s disease (PD) is a progressive neurological disorder that affects individuals worldwide. One of the most distressing aspects of this condition is the inability to use one’s limbs effectively, despite their physical presence. By the time symptoms become clinically evident, approximately 10–15 years have elapsed, during which significant and irreversible neurodegeneration has already occurred. Emerging research characterizes PD as a multifactorial disorder involving complex and interconnected biological pathways, making single-biomarker approaches insufficient for comprehensive detection. Consequently, there is a critical need to develop advanced biosensors capable of simultaneously detecting multiple biomarkers. However, challenges such as biomarker heterogeneity, variability in expression levels, and limited clinical validation continue to hinder the development of robust diagnostic strategies. In this context, optical biosensors have gained prominence due to their exceptional sensitivity, label-free detection capabilities, rapid response times, and compatibility with diverse PD biomarkers. Technologies such as surface plasmon resonance, Raman and surface-enhanced Raman spectroscopy (SERS), and optical fibre-based sensors offer powerful avenues for real-time quantification of α-synuclein proteoforms, inflammatory mediators, and PD-associated nucleic acids. Despite these advantages, issues related to sensor reproducibility, lack of standardization, and challenges in large-scale implementation remain significant barriers, which are critically examined in this article. It also highlights the role of established and emerging biomarkers in PD pathophysiology and explores how optical biosensing technologies provide sensitive, specific, and minimally invasive platforms for early detection. Furthermore, we discuss future prospects, including the development of multiplexed detection systems, integration with microfluidics and wearable technologies.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147827669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrections to: An aptamer-based fluorometric microcystin-LR assay using DNA strand-based competitive displacement","authors":"Raja Chinnappan,&nbsp;Razan AlZabn,&nbsp;Khalid M. Abu-Salah,&nbsp;Mohammed Zourob","doi":"10.1007/s00604-026-08092-6","DOIUrl":"10.1007/s00604-026-08092-6","url":null,"abstract":"","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-free paper-based electrochemical aptasensor with tunable selectivity for assessing neurotransmitter imbalance in Alzheimer’s disease","authors":"Silvia Dortez,&nbsp;Miriam Chávez,&nbsp;Ana Montero-Calle,&nbsp;Rodrigo Barderas,&nbsp;Marta Pacheco,&nbsp;Alberto Escarpa","doi":"10.1007/s00604-026-08100-9","DOIUrl":"10.1007/s00604-026-08100-9","url":null,"abstract":"<div><p>A paper-based electrochemical aptasensor (PEA) is reported for the simultaneous detection of extracellular dopamine (DA) and serotonin (SE) in human brain samples, enabling the assessment of neurotransmitter imbalances associated with Alzheimer’s disease (AD). The PEA integrates a dual-selectivity strategy that combines aptamer-mediated molecular recognition with potential-resolved electrochemical neurotransmitters discrimination, allowing reliable differentiation between healthy and AD-diagnosed left prefrontal cortex tissues based on altered dopaminergic and serotonergic levels. Thiolated aptamers immobilized onto gold nanoparticles (AuNPs) at the paper electrode interface provide selective neurotransmitter biorecognition, while the distinct oxidation potentials of DA (+ 0.10 V) and SE (+ 0.25 V <i>vs</i>. Ag/AgCl) enable their label-free, simultaneous quantification on a single disposable electrode. The PEA read biologically meaningful concentration shifts across a clinically relevant dynamic range, spanning levels characteristic of healthy tissue to those observed in advanced pathological stages. The PEA approach highlights its potential for liquid biopsy–oriented neurochemical monitoring and supports its application in multiplexed biosensing within organ-on-a-chip systems, enabling early diagnostic strategies for neurodegenerative diseases.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture><span>The alternative text for this image may have been generated using AI.</span></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-026-08100-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147829500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}