Microchimica Acta最新文献

Metal/heteroatom-doped carbon-based hybrid material as the matrix for rapid and high-throughput identification of phthalate esters in complex samples by laser desorption/ionization 金属/杂原子掺杂碳基杂化材料作为基体，用于激光解吸/电离快速高通量鉴定复杂样品中的邻苯二甲酸酯

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-29 DOI: 10.1007/s00604-025-07124-x

Luyan Meng, Baichun Wang, Yijia Rong, Shun Zhang, Ting Cai, Chuan-Fan Ding, Yinghua Yan

{"title":"Metal/heteroatom-doped carbon-based hybrid material as the matrix for rapid and high-throughput identification of phthalate esters in complex samples by laser desorption/ionization","authors":"Luyan Meng, Baichun Wang, Yijia Rong, Shun Zhang, Ting Cai, Chuan-Fan Ding, Yinghua Yan","doi":"10.1007/s00604-025-07124-x","DOIUrl":"10.1007/s00604-025-07124-x","url":null,"abstract":"<div><p>A novel matrix of metal particles and metal oxides modified N-doped multi-walled carbon nanotubes (N-MWCNTs/AuNPs/Co<sub>3</sub>O<sub>4</sub>) is introduced for the rapid detection of phthalate esters (PAEs) using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). By leveraging the synergistic effects of metal/heteroatom-doped carbon-based components, this innovative hybrid material demonstrated exceptional ionization efficiency for PAEs. Specifically, it enables the detection of bis(2-ethylhexyl) phthalate (DEHP) at concentrations as low as of 1 µg·mL⁻<sup>1</sup>. Moreover, the N-MWCNTs/AuNPs/Co<sub>3</sub>O<sub>4</sub> composite not only surpassed conventional organic matrices in terms of ionization efficiency and reproducibility, but also facilitated the effective enrichment of PAEs, achieving an enrichment efficiency range from 94.1% ± 5.8% to 98.2% ± 2.3%. Furthermore, this method was successfully applied to detect DEHP in human serum and urine samples, with urinary DEHP concentrations estimated ranging from 29.2 to 38.4 ng·mL⁻<sup>1</sup>. This approach represents a promising strategy for high-throughput PAE detection, offering significant potential for applications in environmental and biomedical analysis.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical determination of folic acid in triple-matrix biofluids: a PAni-DES and Au/Printex L6@Chitosan enhanced lab-made screen-printed sensor 三基质生物体液中叶酸的电化学测定：PAni-DES和Au/Printex L6@Chitosan增强实验室制造的丝网印刷传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-28 DOI: 10.1007/s00604-025-07177-y

Igor G. S. Oliveira, Fernando C. Gallina, Muriel R. Affonso, Robson S. Souto, Marcos R. V. Lanza, Silvia M. Martelli, Willyam R. P. Barros

{"title":"Electrochemical determination of folic acid in triple-matrix biofluids: a PAni-DES and Au/Printex L6@Chitosan enhanced lab-made screen-printed sensor","authors":"Igor G. S. Oliveira, Fernando C. Gallina, Muriel R. Affonso, Robson S. Souto, Marcos R. V. Lanza, Silvia M. Martelli, Willyam R. P. Barros","doi":"10.1007/s00604-025-07177-y","DOIUrl":"10.1007/s00604-025-07177-y","url":null,"abstract":"<div><p> The development and application of a highly sensitive electrochemical screen-printed electrode (SPE) for the determination of folic acid (FA) is presented. The SPE was fabricated on a polyethylene substrate using customized graphite ink and modified with Printex L6 carbon (PL6C) dispersed in chitosan (Chi). Gold (Au) nanoparticles were electrodeposited onto the SPE surface, followed by electropolymerization of polyaniline (PAni) in a deep eutectic solvent (DES), enhancing the electrochemical performance. The modified PAni-DES/Au/PL6C@Chi/SPE was characterized using cyclic voltammetry and differential pulse voltammetry in 0.1 mol L⁻<sup>1</sup> Britton-Robinson buffer solution at pH 7.0. Compared to the bare SPE, the modified sensor showed a significantly enhanced current response for FA detection, with a linear dynamic range of 1.15 to 40.00 μmol L⁻<sup>1</sup> (<i>R</i><sup>2</sup> = 0.998) and a limit of detection (LOD) of 0.018 μmol L⁻<sup>1</sup>. The sensor was successfully applied to real samples such as saliva, blood serum, and urine, exhibiting high sensitivity with recovery between 95.25% and 103.80%. It also demonstrated excellent selectivity against common interferents, and the fully lab-made platform offers competitive performance compared to conventional systems, highlighting its innovative design and potential for customization. The absence of a significant memory effect and minimal matrix interference further underscore the sensor’s reliability for clinical diagnoses and quality control analyses. Additionally, the sensor is cost-effective and requires low reagent and sample consumption, making it a promising alternative for practical applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric determination of tyrosinase activity and high-throughput screening of inhibitors based on in-situ formation of gold nanoparticles 酪氨酸酶活性的比色测定及基于原位形成金纳米颗粒的抑制剂的高通量筛选

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-28 DOI: 10.1007/s00604-025-07155-4

Guohua Zhou, Zhicheng Liu, Yuning Huang, Rufei Yang, Xin Guo, Yongmei Jia, Peilian Liu, Zhiguo Li, Wenjuan Cui

{"title":"Colorimetric determination of tyrosinase activity and high-throughput screening of inhibitors based on in-situ formation of gold nanoparticles","authors":"Guohua Zhou, Zhicheng Liu, Yuning Huang, Rufei Yang, Xin Guo, Yongmei Jia, Peilian Liu, Zhiguo Li, Wenjuan Cui","doi":"10.1007/s00604-025-07155-4","DOIUrl":"10.1007/s00604-025-07155-4","url":null,"abstract":"<div><p> A colorimetric method is presented for detecting tyrosinase activity and screening inhibitors, leveraging the in-situ formation of gold nanoparticles mediated by catechol. The active tyrosinase converses catechol into o-quinone leading to changes in both the size of AuNPs and their corresponding UV–Vis absorption spectrum as well as visual color. This enzyme-mediated alteration can be modulated by tyrosinase inhibitors through chemical regulation of enzyme activity. Based on these, facile detection of tyrosinase and high-throughput screening for its inhibitors can be achieved simply by visually observing color changes in solution. The proposed method is characterized by its sensitivity, simplicity, rapidity, and accuracy, allowing for quantitative determination of tyrosinase within the range 0.5 to 25 U/mL, with a detection limit of 0.038 U/mL. Furthermore, visual or instrumental observation of the color changes in gold nanoparticles enables high-throughput screening of inhibitors and calculation of their IC50 values. We anticipate that this approach will significantly contribute to tyrosinase research and facilitate the development, discovery, and high-throughput screening of effective inhibitors.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An electrochemiluminescence sensor based on Ru(bpy)32+@Au and GO@CdS for detection of tartrazine 基于Ru(bpy)32+@Au和GO@CdS的酒石黄检测电化学发光传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-27 DOI: 10.1007/s00604-025-07144-7

Li Tian, Huiling Li, Pengfei Han, Guangping Ma, Wenzhuo Wang, Hanyue Jiang, Juan Lu

{"title":"An electrochemiluminescence sensor based on Ru(bpy)32+@Au and GO@CdS for detection of tartrazine","authors":"Li Tian, Huiling Li, Pengfei Han, Guangping Ma, Wenzhuo Wang, Hanyue Jiang, Juan Lu","doi":"10.1007/s00604-025-07144-7","DOIUrl":"10.1007/s00604-025-07144-7","url":null,"abstract":"<div><p>An electrochemiluminescence (ECL) sensor was constructed based on Ru(bpy)<sub>3</sub><sup>2+</sup>@Au and GO@CdS for the detection of tartrazine (TTZ). In the presence of the co-reactant TPrA, Ru(bpy)<sub>3</sub><sup>2+</sup> and CdS quantum dots (QDs) were used as dual luminophores to synergistically amplify the ECL signal of the sensor. Gold nanoparticles (Au NPs) enhanced the electron transport capability of the system and thereby amplified the ECL intensity. Graphene oxide (GO), as a carrier, has a special wrinkle-like structure that not only adsorbed a substantial amount of CdS QDs but also prevented their agglomeration. Additionally, a more sensitive detection method was established based on the quenching of the ECL signal, which occurred due to the interaction between Ru(bpy)<sub>3</sub><sup>2+</sup> and TTZ. A low detection limit (1.3 × 10<sup>–13</sup> mol·L<sup>−1</sup>, S/N = 3) was obtained for TTZ concentrations ranging from 4.0 × 10<sup>–13</sup> to 4.0 × 10<sup>–8</sup> mol·L<sup>−1</sup>. Finally, actual measurement results from three beverage samples demonstrated that the prepared sensors possess excellent practical capabilities for TTZ detection.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemiluminescent/colorimetric monitoring 25(OH)D3 based on HOFs-g-C3N4-CeO2 functional composite 基于hfs -g- c3n4 - ceo2功能复合材料的电化学发光/比色监测25(OH)D3

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-26 DOI: 10.1007/s00604-025-07172-3

Meng Zhang, Yinan Li, Dalin Xu, Wenyue Wang, Hong Zhou, Xiaoyan Yang

{"title":"Electrochemiluminescent/colorimetric monitoring 25(OH)D3 based on HOFs-g-C3N4-CeO2 functional composite","authors":"Meng Zhang, Yinan Li, Dalin Xu, Wenyue Wang, Hong Zhou, Xiaoyan Yang","doi":"10.1007/s00604-025-07172-3","DOIUrl":"10.1007/s00604-025-07172-3","url":null,"abstract":"<div><p>Vitamin D, mainly in the active form of 25-hydroxy vitamin D<sub>3</sub> (25(OH)D<sub>3</sub>), participates in blood circulation and plays a vital role in metabolic homeostasis. Herein, an electrochemiluminescent (ECL)/colorimetric dual-analytical biosensing platform based on HOFs-g-C<sub>3</sub>N<sub>4</sub>-CeO<sub>2</sub> (HCC) composite was developed for 25(OH)D<sub>3</sub> detection. In the ECL system, electrochemiluminescence-resonance energy transfer (ECL-RET) occurred utilizing HCC as the ECL donor and gold nanorods (Au NRs) as the acceptor. The binding of the complementary aptamer chain (ssDNA) labeled with Au NRs to the aptamer chain VDBA14 immobilized on the surface of HCC quenched the ECL emission via ECL-RET. After adding the target 25(OH)D<sub>3</sub>, ECL response was recovered due to the competition for VDBA14 between ssDNA and 25(OH)D<sub>3</sub>, resulting in the reduction of Au NRs and attenuation of the ECL-RET on the electrode. In the colorimetric system, VDBA14 immobilized on magnetic beads (MBs) captured ssDNA tagged with HCC through complementary base pairing. VDBA14 could specifically recognize 25(OH)D<sub>3</sub> to release ssDNA competitively, and then, the released HCC was used as the nanozyme to catalyze the chromogenic reaction between tetramethylbenzidine (TMB) and H<sub>2</sub>O<sub>2</sub>. The bimodal analysis showed a good response to 25(OH)D<sub>3</sub> in the linear range 20 ~ 80 ng/mL, with detection limits of 15.91 ng/mL (for the ECL system) and 7.28 ng/mL (for the colorimetric system). The proposed dual-analytical biosensor improved the reliability of detecting 25(OH)D<sub>3</sub> and performed satisfactorily in practical applications, which is of great significance in immunomodulation, skin physiology, and other important aspects of human health.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical immunosensor based on poly(5-aminoindole) for cancer biomarker pentraxin-3 detection 基于聚（5-氨基吲哚）的肿瘤生物标志物戊烷素-3检测电化学免疫传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-25 DOI: 10.1007/s00604-025-07174-1

Katesara Phasuksom, Natthaporn Thongwattana, Nuttha Ariyasajjamongkol, Napa Parinyanitikul, Anuvat Sirivat

{"title":"Electrochemical immunosensor based on poly(5-aminoindole) for cancer biomarker pentraxin-3 detection","authors":"Katesara Phasuksom, Natthaporn Thongwattana, Nuttha Ariyasajjamongkol, Napa Parinyanitikul, Anuvat Sirivat","doi":"10.1007/s00604-025-07174-1","DOIUrl":"10.1007/s00604-025-07174-1","url":null,"abstract":"<div><p>Pentraxin-3 (PTX-3) plays a critical role in regulating inflammation, immune response, angiogenesis, and tissue remodeling. As a serum biomarker, it holds a potential in cancer diagnostics and monitoring. Elevated expression of PTX-3 has been found in various cancers, namely lung, prostate, gastric, breast, and hepatic cancers. Here, a PTX-3 immunosensor was fabricated by utilizing poly(5-aminoindole) (P5AIn) to increase the current response and the electroactive surface area (ESA). (3-Aminopropyl)triethoxysilane (APTES) was used as a linker and as an electron transfer enhancer. The developed PTX-3 immunosensor (BSA/anti-PTX-3/APTES/P5AIn/SPCE) shows excellent performance for PTX-3 detection in a wide concentration range from 0.0001 to 2000 ng/mL with the LOD of 0.012 pg/mL. Furthermore, the sensor possessed good reproducibility (%RSD = 0.78), a long shelf-life of at least 4 weeks (% RSD = 0.89), and high specificity to PTX-3. The fabricated PTX-3 immunosensor was demonstrated in the measurement of PTX-3 concentrations in real blood serums from gastric patients. It has been shown as a potentially promising portable sensor for cancer diagnostics and monitoring for cancer patients in remote areas with a low cost.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dual-channel lipid droplet-targeting probe for the detection of polarity and HOCl in models of NAFLD, inflammation, and cancer 一种双通道脂滴靶向探针，用于检测NAFLD、炎症和癌症模型中的极性和HOCl

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-25 DOI: 10.1007/s00604-025-07171-4

Tingting Yang, Shiyu Yin, Huina Liu, Shunyi Wang, Chengqi Wang, Yuxin Chen, Xiaoya Liu, Fangke Zhu, Jiaxuan Guo, Lajiatai, Dongzhi Suonanmu, Qingjia Ren, Shenghua Liao, Caolong Li

{"title":"A dual-channel lipid droplet-targeting probe for the detection of polarity and HOCl in models of NAFLD, inflammation, and cancer","authors":"Tingting Yang, Shiyu Yin, Huina Liu, Shunyi Wang, Chengqi Wang, Yuxin Chen, Xiaoya Liu, Fangke Zhu, Jiaxuan Guo,  Lajiatai, Dongzhi Suonanmu, Qingjia Ren, Shenghua Liao, Caolong Li","doi":"10.1007/s00604-025-07171-4","DOIUrl":"10.1007/s00604-025-07171-4","url":null,"abstract":"<div><p>Abnormal lipid droplet accumulation and metabolism can precipitate various diseases. Understanding the dynamics of intracellular lipid droplets, particularly hypochlorite acid (HOCl) and polarity, is crucial for elucidating the onset and progression of these diseases. In this study, we synthesized a near-infrared fluorescent probe, <b>NSSP</b>, featuring a D-π-A structure, which facilitates the independent dual-response detection of HOCl and polarity. <b>NSSP</b> not only reveals the polarity and HOCl fluctuations in inflammatory cells and inflammation in live mice but also differentiates between normal and fatty liver tissues through two distinct channels. Furthermore, the probe has proven effective in identifying neoplasms within live murine models at both the cellular and systemic levels, thereby affirming its utility as an instrumental resource for the precocious detection of carcinomas. This offers a hopeful avenue for the diagnostic scrutiny of pathologies.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of octyl-modified silica isoporous membrane for hydrophobic enrichment and electrochemical detection of methylmercury in aquatic products 制备疏水富集和电化学检测水产品中甲基汞的辛基改性二氧化硅异孔膜

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-24 DOI: 10.1007/s00604-025-07169-y

Guiyun Zhang, Zhiwei Lai, Jianhua Xie, Weiqiang Yang

{"title":"Preparation of octyl-modified silica isoporous membrane for hydrophobic enrichment and electrochemical detection of methylmercury in aquatic products","authors":"Guiyun Zhang, Zhiwei Lai, Jianhua Xie, Weiqiang Yang","doi":"10.1007/s00604-025-07169-y","DOIUrl":"10.1007/s00604-025-07169-y","url":null,"abstract":"<div><p>Methylmercury (CH<sub>3</sub>Hg<sup>+</sup>), a lipophilic environmental pollutant, accumulates in fish, shellfish, and other organisms, posing significant risks to human health through the food chain. Developing a convenient and sensitive analytical method for CH<sub>3</sub>Hg<sup>+</sup> detection is crucial for reducing costs and enhancing the efficiency of food safety testing. In this study, we prepared an octyl-modified silica isoporous membrane on the indium tin oxide (ITO) electrode (Octyl-SIM/ITO) via the electrochemical-assisted self-assembly (EASA) method using octyltrimethoxysilane (O-TES) as the functional organosilane. The Octyl-SIM/ITO electrode exhibits vertically-ordered nanochannels and strong hydrophobic affinity, enabling selective penetration and enrichment of weakly polar analytes. Utilizing square wave anodic stripping voltammetry (SWASV), the Octyl-SIM/ITO electrode demonstrates superior electrochemical response signals for CH<sub>3</sub>Hg<sup>+</sup> detection, achieving a detection limit as low as 4 nM. This method allows for accurate and reproducible detection of CH<sub>3</sub>Hg<sup>+</sup> in fish and oyster samples with minimal sample preparation, offering promising potential for portable in situ detection.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of immobilized xanthine oxidase with magnetic metal–organic framework and its application in screening of active ingredients in traditional Chinese medicine 磁性金属有机骨架固定化黄嘌呤氧化酶的制备及其在中药活性成分筛选中的应用

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-24 DOI: 10.1007/s00604-025-07167-0

Chenxi Wu, Na Zhang, Hailin Li, Hao Wang, Lifeng Han, Yuefei Wang, Fuyi Li, Fei Tian

{"title":"Preparation of immobilized xanthine oxidase with magnetic metal–organic framework and its application in screening of active ingredients in traditional Chinese medicine","authors":"Chenxi Wu, Na Zhang, Hailin Li, Hao Wang, Lifeng Han, Yuefei Wang, Fuyi Li, Fei Tian","doi":"10.1007/s00604-025-07167-0","DOIUrl":"10.1007/s00604-025-07167-0","url":null,"abstract":"<div><p>Enzymes play a crucial role in the development and progression of various diseases, making them important targets for drug development. However, the stability issues associated with natural enzymes limit their broader application. Traditional methods for screening enzyme inhibitors from natural products are often time-consuming and labor-intensive. In this study, we designed and employed magnetic metal–organic frameworks (MOFs) to immobilize xanthine oxidase for the first time. By leveraging the porous structure and high specific surface area of MOFs, combined with the magnetic responsiveness of nanoparticles, we successfully developed a novel method for the efficient screening of potential enzyme inhibitors derived from natural products. By using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) as a cross-linking agent, we achieved efficient immobilization of xanthine oxidase and identified baicalin as a potential inhibitor from the extract of <i>Scutellaria baicalensis</i>. In addition, we confirmed the adsorption capacity of this method for hordenine, demonstrated the specific adsorption of allopurinol, and also performed in vitro activity validation for baicalein. We not only successfully prepared the immobilized enzyme but also showcased that this method can efficiently screen and isolate potential enzyme inhibitors from traditional Chinese medicine, which provides a rapid and efficient new strategy for identifying enzyme inhibitors in natural products. This innovative approach offers a fresh perspective on the application of botanical medicine and the pharmacological treatment of hyperuricemia, which has important theoretical and practical significance.</p>\u0000<div><figure><div><div><picture><source><img></source></picture></div><div><p>Schematic diagram of synthesis of XO@MMOF (<b>A</b>) and ligand fishing process (<b>B</b>)\u0000</p></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MOF-engineered Cu2O nanozymes with boosted peroxidase-like activity for colorimetric-fluorescent dual-mode detection of deoxynivalenol mof工程的Cu2O纳米酶具有增强过氧化物酶样活性，用于比色-荧光双模式检测脱氧雪腐镰刀菌醇

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-24 DOI: 10.1007/s00604-025-07140-x

Xiaodong Zhu, Yangchun He, Xinhua Xie, Bobo Zhang, Junhao Wang, Haoran Shen, Yingju Liu, Huifu Ji, Hongshuai Zhu

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