Microchimica Acta最新文献

Dual-channel fluorescence biosensor for simultaneous miR-133a and ATP detection in living muscle cells. 用于同时检测活肌细胞中miR-133a和ATP的双通道荧光生物传感器。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-31 DOI: 10.1007/s00604-025-07364-x

Liandong Zhao, Mingzhu Zhao, Jing Wang, Shuqi Zhang, Rong-Mei Kong, Jianxin Yang, Yan Zhao, Weiheng Kong

{"title":"Dual-channel fluorescence biosensor for simultaneous miR-133a and ATP detection in living muscle cells.","authors":"Liandong Zhao, Mingzhu Zhao, Jing Wang, Shuqi Zhang, Rong-Mei Kong, Jianxin Yang, Yan Zhao, Weiheng Kong","doi":"10.1007/s00604-025-07364-x","DOIUrl":"https://doi.org/10.1007/s00604-025-07364-x","url":null,"abstract":"Muscle injuries present a significant challenge in sports and physical activity, necessitating advanced diagnostic tools. Current invasive methods, such as muscle biopsies, are limited by procedural risks and lack of dynamic data. In this study, we developed a dual-channel fluorescent biosensor based on MoS2 nanosheets and fluorescence resonance energy transfer (FRET) for the simultaneous detection of miR-133a and ATP in living mouse myoblast cells. The design leverages the pathophysiological roles of miR-133a (a biomarker upregulated during muscle injury) and ATP (a critical indicator of energy metabolism). The biosensor incorporates FAM-labeled miR-133a-specific ssDNA and Cy5-labeled ATP aptamer probes immobilized on MoS2 nanosheets, achieving fluorescence quenching via FRET. Target binding triggered fluorescence recovery, enabling sensitive detection with limits of detection (LOD) of 3.3 pM for miR-133a and 0.33 µM for ATP. In vitro experiments confirmed high specificity and biocompatibility in mouse myoblasts, validated through confocal microscopy and flow cytometry. This platform provides a foundational tool for monitoring molecular dynamics associated with muscle injury, focusing on miR-133a and ATP levels in cellular environments. This work addresses limitations of traditional invasive diagnostics and lays the groundwork for potential applications in sports medicine research.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"543"},"PeriodicalIF":5.3,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature-responsive AuNPs/poly(N-isopropylacrylamide) hydrogel-based sensor for SERS detection of paraquat in fruit juices. 温度响应型AuNPs/聚n -异丙基丙烯酰胺水凝胶传感器用于果汁中百草枯的SERS检测。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-31 DOI: 10.1007/s00604-025-07366-9

Jingyi Fan, Yiqun Huang, Junjian Miao, Keqiang Lai

{"title":"Temperature-responsive AuNPs/poly(N-isopropylacrylamide) hydrogel-based sensor for SERS detection of paraquat in fruit juices.","authors":"Jingyi Fan, Yiqun Huang, Junjian Miao, Keqiang Lai","doi":"10.1007/s00604-025-07366-9","DOIUrl":"https://doi.org/10.1007/s00604-025-07366-9","url":null,"abstract":"A novel composite SERS substrate was developed based on gold nanoparticles (AuNPs) and poly(N-isopropylacrylamide) (PNIPAM) hydrogel. PNIPAM hydrogel is a temperature-responsive material with a lower critical solution temperature (LCST) of about 32 °C, regulating its swelling-shrinking behavior by changing temperature to evaluate the enrichment efficiency of target molecules. Experimental results demonstrated that the substrate exhibited a swelling ratio of 400-600% at room temperature, facilitating the efficient enrichment of paraquat molecules. The optimal Raman response was observed when the substrate contained a NIPAM concentration of 0.8 mol/L and an AuNPs concentration of 300%. Additionally, the substrate exhibited excellent detection performance, including high sensitivity, good reproducibility, uniformity, and temporal stability. The minimum detectable concentration of paraquat in standard solutions was 0.1 μg/L. In apple juice and orange juice, the detection limit ranged from 0.072 to 0.112 μg/L, with spiked recoveries of 85.55%-110.55% and 88.57%-107.15%, respectively. This study presents a highly selective and reliable SERS sensing strategy for the rapid detection of trace paraquat in juice samples.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"542"},"PeriodicalIF":5.3,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the total antioxidant capacity of Anchusa italica retz using carbon fiber microelectrode modified by Au-Ag alloy urchin-like nanostructures. 用金-银合金类海胆纳米结构修饰的碳纤维微电极评价海胆总抗氧化能力。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-31 DOI: 10.1007/s00604-025-07396-3

Han Cheng, Tao Fang, Yu Li, Uchkun Ishimov, Awais Ihsan, Yanbing Pan, Ruiting Tian, Jun Li, Syed Aftab Naqvi, Xianju Huang

{"title":"Evaluation of the total antioxidant capacity of Anchusa italica retz using carbon fiber microelectrode modified by Au-Ag alloy urchin-like nanostructures.","authors":"Han Cheng, Tao Fang, Yu Li, Uchkun Ishimov, Awais Ihsan, Yanbing Pan, Ruiting Tian, Jun Li, Syed Aftab Naqvi, Xianju Huang","doi":"10.1007/s00604-025-07396-3","DOIUrl":"https://doi.org/10.1007/s00604-025-07396-3","url":null,"abstract":"Anchusa italica Retz, a plant rich in bioactive compounds, has garnered considerable attention for its antioxidant properties and associated health benefits. However, the traditional methods for assessing antioxidant capacity often suffer from limited sensitivity and operational complexity. To overcome these challenges, gold-silver alloy urchin-like nanostructures (AAA-UNs) were synthesized using an ascorbic acid reduction method and electrodeposited onto a carbon fiber microelectrode (CFME) to form a highly sensitive electrochemical sensor (AAA-UNs/CFME). The morphology and structure of the synthesized material and modified electrode were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Sensor performance was optimized using cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). The antioxidant capacity of Anchusa italica Retz was evaluated by monitoring changes in the electrochemical behavior of guanine (used as a model biomolecule) upon exposure to Fenton-generated hydroxyl radicals (·OH), with and without the protective presence of antioxidants. The AAA-UNs/CFME exhibited high sensitivity, stability, and anti-interference capability, achieving a detection limit of 0.05 mmol/L and a linear range of 0.3-3.0 mmol/L for guanine. Experimental results confirmed that Anchusa italica Retz extracts from different geographic origins possess significant antioxidant activity. Among these, the Xinjiang Anchusa italica Retz extract showed the highest radical scavenging efficiency (81.05%), followed by samples from Pakistan (69.63%) and Hainan (60.26%). These findings underscore the innovative application of the AAA-UNs/CFME sensor for reliable, rapid and sensitive assessment of antioxidant capacity in complex botanical samples.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"544"},"PeriodicalIF":5.3,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly stable benzonitrile functionalized covalent organic framework coating for solid-phase microextraction of hydroxyphenyl esters in complex matrix samples. 高稳定的苯腈功能化共价有机骨架涂层用于复合基质样品固相微萃取羟基苯基酯。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-30 DOI: 10.1007/s00604-025-07419-z

Zong-Mu Dong, Liming Qin, Yaqing Guo, Miaoqing Xu, Jian-Feng Wu, Guang-Chao Zhao

{"title":"Highly stable benzonitrile functionalized covalent organic framework coating for solid-phase microextraction of hydroxyphenyl esters in complex matrix samples.","authors":"Zong-Mu Dong, Liming Qin, Yaqing Guo, Miaoqing Xu, Jian-Feng Wu, Guang-Chao Zhao","doi":"10.1007/s00604-025-07419-z","DOIUrl":"https://doi.org/10.1007/s00604-025-07419-z","url":null,"abstract":"A mesoporous surface benzonitrile functionalized covalent organic framework (TpBD-CN-COF) was synthesized as a novel solid-phase microextraction (SPME) fiber coating for the extraction of six hydroxyphenyl esters (PBs), and combined with gas chromatography-flame electric-ion detector (GC-FID). PBs were sensitively determined in aqueous solution. Due to its high specific surface area, high chemical stability in both strongly acidic and alkaline media, and in polar or non-polar media, as well as its high thermal stability, the TpBD-CN-COF fiber coating showed excellent extraction performances for PBs with an enrichment factor twice as high as that of TpBD-COF. Some parameters that influenced the extraction performance, such as extraction time and temperature, pH, and stirring speed were investigated. Under optimal conditions, a wide linear range (0.06-500 μg L-1), low limits of detection (LOD, 0.003-0.042 μg L-1) were obtained for PBs determination. The relative standard deviations (RSDs, n = 6) for intra-day, inter-day, and fiber-to-fiber were less than 8.06%, 7.8%, and 8.6%, respectively. Furthermore, the analytical method could be successfully used to detect six PBs in cosmetics and environmental water samples, achieving satisfactory recoveries ranging from 85.14 to 107.67%.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"540"},"PeriodicalIF":5.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical DNA biosensor based on a novel pyrrolidinyl peptide nucleic acid sequence immobilized on a graphene oxide/bovine serum albumin-modified electrode for the direct diagnosis of seed-borne pathogen. 基于新型吡咯烷基肽核酸序列固定在氧化石墨烯/牛血清白蛋白修饰电极上的电化学DNA生物传感器用于直接诊断种传病原体。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-30 DOI: 10.1007/s00604-025-07371-y

Hendra Hendra, Wiphawee Leesutthiphonchai, Nattakorn Kuncharoen, Supot Kasem, Orawon Chailapakul, Tugba Ozer, Sakda Jampasa, Tiyakhon Chatnaparat

{"title":"Electrochemical DNA biosensor based on a novel pyrrolidinyl peptide nucleic acid sequence immobilized on a graphene oxide/bovine serum albumin-modified electrode for the direct diagnosis of seed-borne pathogen.","authors":"Hendra Hendra, Wiphawee Leesutthiphonchai, Nattakorn Kuncharoen, Supot Kasem, Orawon Chailapakul, Tugba Ozer, Sakda Jampasa, Tiyakhon Chatnaparat","doi":"10.1007/s00604-025-07371-y","DOIUrl":"https://doi.org/10.1007/s00604-025-07371-y","url":null,"abstract":"Xanthomonas oryzae pv. oryzae (Xoo) is a significant pathogen responsible for seed-borne diseases that negatively impact rice yields globally. Effective early detection methods for this pathogen, particularly those based on nucleic acid detection, are crucial for maintaining plant health. This study presented an electrochemical DNA biosensor that utilized a novel pyrrolidinyl peptide nucleic acid (acpcPNA) probe sequence derived from the wxoC gene, which encoded a putative glycosyltransferase, for sensitive, selective, and direct detection of Xoo in rice seed samples. The acpcPNA probe was designed, synthesized, and characterized using mass spectrometry and melting temperature analysis. It was then immobilized onto a graphene oxide-bovine serum albumin (GO/BSA) nanocomposite modified electrode to demonstrate its electrochemical sensing capabilities, as GO/BSA aided in stabilizing film formation, leading to an effective immobilization process. The formation of the probe with Xoo double-stranded DNA (dsDNA) via duplex invasion considerably reduced the redox signal response due to electrostatic repulsion and insulating properties. With the optimized conditions, the sensor demonstrated a linear detection range from 10 to 20 × 103 nM for synthetic DNA and 103 to 108 CFU/mL for bacterial cell culture, with detection and quantification limits of 1.8 and 10 nM, and 1 × 103 and 3.3 × 103 CFU/mL, respectively. This biosensor successfully detected Xoo dsDNA in artificially and naturally infected rice seeds without pre-enrichment and denaturation in a label-free format within 45 min, as verified by polymerase chain reaction. Furthermore, the system offered exceptional specificity, making it a promising tool for the early detection of this pathogen.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"541"},"PeriodicalIF":5.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutting-edge advances in two-dimensional layered double hydroxides: structure, synthesis, functionalization, characterization, and advanced sensor applications. 二维层状双氢氧化物的前沿进展：结构、合成、功能化、表征和先进的传感器应用。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-29 DOI: 10.1007/s00604-025-07357-w

Kanwal Iqbal, Anam Iqbal, Sara Benabid, Zeeshan Ajmal

{"title":"Cutting-edge advances in two-dimensional layered double hydroxides: structure, synthesis, functionalization, characterization, and advanced sensor applications.","authors":"Kanwal Iqbal, Anam Iqbal, Sara Benabid, Zeeshan Ajmal","doi":"10.1007/s00604-025-07357-w","DOIUrl":"10.1007/s00604-025-07357-w","url":null,"abstract":"Two-dimensional layered double hydroxides (LDHs) have emerged as highly promising materials for sensor applications due to their tunable composition, large specific surface area, and excellent ion-exchange capacity. This review provides a focused and up-to-date overview of the structural features, synthesis methods, and functionalization strategies of LDHs, specifically in the context of their sensor-related properties. Unlike prior studies, this work systematically explores how the design of LDH-based sensing systems influences their performance and practical applications. Recent advancements in electrochemical, gas, optical, and humidity sensors are critically analyzed, with insights into performance advantages and underlying sensing mechanisms. Challenges such as poor conductivity and limited long-term stability are discussed in relation to material design strategies. Additionally, the review outlines emerging trends, including the development of multifunctional LDH-based sensors and their integration into intelligent sensing platforms. By bridging recent experimental progress with theoretical insights, this review delivers a unique and application-oriented perspective, offering valuable guidance for the rational design of LDHs in next-generation sensing technologies.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"535"},"PeriodicalIF":5.3,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic aptamer/molecularly imprinted polymer-driven fluorescence/colorimetric dual-mode sensor based on Au/ZIF-67 nanocomposites for ultrasensitive ochratoxin A detection. 基于Au/ZIF-67纳米复合材料的协同适体/分子印迹聚合物驱动荧光/比色双模传感器用于超灵敏赭曲霉毒素A检测。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-29 DOI: 10.1007/s00604-025-07346-z

Yufan Yang, Bo Zhao, Xiaopeng Hu, Huiwen Gu, Xiu-Fang Yan, Xiaoli Yin

{"title":"Synergistic aptamer/molecularly imprinted polymer-driven fluorescence/colorimetric dual-mode sensor based on Au/ZIF-67 nanocomposites for ultrasensitive ochratoxin A detection.","authors":"Yufan Yang, Bo Zhao, Xiaopeng Hu, Huiwen Gu, Xiu-Fang Yan, Xiaoli Yin","doi":"10.1007/s00604-025-07346-z","DOIUrl":"10.1007/s00604-025-07346-z","url":null,"abstract":"A dual-mode fluorescent/colorimetric sensor is presented utilizing Au/ZIF-67 nanocomposites for an ultrasensitive ochratoxin A (OTA) detection. The system employs polydopamine-coated Fe3O4 nanoparticles (Fe3O4@MIP) as magnetic recognition elements and aptamer/3,3',5,5'-tetramethylbenzidine (TMB)-functionalized Au/ZIF-67 nanocomposites (Apt/TMB/Au/ZIF-67) as dual-signal probes for both fluorescence and colorimetric detection. Through molecular imprinting-aptamer synergistic recognition, OTA binding induces the formation of sandwich-structured particles (Fe3O4@MIP-OTA-Apt/TMB/Au/ZIF-67). Magnetic separation of these particles facilitates fluorescence measurements, while residual Apt/TMB/Au/ZIF-67 in solution permits simultaneous colorimetric detection. Crucially, fluorescence intensity increases while colorimetric absorption decreases proportionally with OTA concentration (0.5-25 ng mL⁻1), enabling self-validating dual-mode detection without requiring reagent replacement. The platform achieves detection limits of 0.15 ng mL⁻1 (fluorescence) and 0.13 ng mL⁻1 (colorimetry). The sensor was successfully validated for OTA quantification in real food samples, demonstrating significant potential for food safety applications.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"533"},"PeriodicalIF":5.3,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ozone-assisted cataluminescence sensor based on morphology-controlled TiO₂@Mg-MOF-74 composite for rapid detection of N-hexane. 基于形态控制TiO2@Mg-MOF-74复合材料的臭氧辅助催化发光传感器快速检测正己烷。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-29 DOI: 10.1007/s00604-025-07389-2

Zhaoxia Shi, Zhen Luo, Yilu Liu, Zi Liu, Xinyue Chen, Yintang Zhang, Yanli Zhou, Maotian Xu

{"title":"Ozone-assisted cataluminescence sensor based on morphology-controlled TiO2@Mg-MOF-74 composite for rapid detection of N-hexane.","authors":"Zhaoxia Shi, Zhen Luo, Yilu Liu, Zi Liu, Xinyue Chen, Yintang Zhang, Yanli Zhou, Maotian Xu","doi":"10.1007/s00604-025-07389-2","DOIUrl":"10.1007/s00604-025-07389-2","url":null,"abstract":"Four different morphologies of TiO2 were successfully synthesized and the morphological dependence of the catalytic performance was investigated for monitoring of n-hexane. TiO2 nanotubes showed the best catalytic performance for the cataluminescence (CTL) reaction of n-hexane because of their high specific surface area and ordered arrangement. To further enhance catalytic activity, a TiO2 nanotube@Mg-MOF-74 composite was synthesized, which was used to construct a CTL sensor for rapid detection of n-hexane rapidly; the introduction of Mg-MOF-74 improved the adsorption capacity and selectivity. A CTL sensor was constructed based on TiO2 nanotube@Mg-MOF-74 for the rapid detection of n-hexane, Under the optimal conditions there was a good linear relationship between CTL signal intensity and the concentration of n-hexane in the range 0.20-200.0 mg/L. The detection limit (LOD, S/N = 3) was 0.08 mg/L. The RSD values for 11 consecutive measurements using the same sensor and 7 different sensors were 2.8% (n = 11) and 3.5% (n = 7), respectively, demonstrating good repeatability and reproducibility. Finally, the sensor was used to detect n-hexane in air samples from different workplaces, with recoveries ranging from 90.2 to 106.4%, showing good accuracy. Thus, the developed CTL sensor could be used for rapid detection of n-hexane in air, avoiding damage to health caused by excessive inhalation.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"534"},"PeriodicalIF":5.3,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Precision photodamage of RNA and mitochondria for cancer therapy with upconversion nanoparticles. 上转化纳米颗粒对RNA和线粒体的精确光损伤治疗。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-29 DOI: 10.1007/s00604-025-07332-5

Shuwen Cheng, Menghang Chen, Yihan Wu, Jinliang Liu, Xiaoqin Zhong, Yong Zhang, Qingqing Dou

{"title":"Precision photodamage of RNA and mitochondria for cancer therapy with upconversion nanoparticles.","authors":"Shuwen Cheng, Menghang Chen, Yihan Wu, Jinliang Liu, Xiaoqin Zhong, Yong Zhang, Qingqing Dou","doi":"10.1007/s00604-025-07332-5","DOIUrl":"10.1007/s00604-025-07332-5","url":null,"abstract":"Photodynamic therapy (PDT) is an emerging approach for cancer treatment that circumvents the discomfort associated with surgical interventions; however, its therapeutic effectiveness remains constrained. In this study, an innovative nanoplatform is introduced that is designed to enhance the efficacy of PDT by specifically targeting RNA and mitochondria, along with providing real-time in vivo imaging capabilities. The nanoplatform is constructed from a multifunctional nanocomposite, UCNP@PEI-RB-furan (referred to as UPRf), integrates several critical components: upconversion nanoparticles (UCNPs) to facilitate light penetration into deep tissue, Rose Bengal (RB) to generate reactive oxygen species (ROS) including singlet oxygen (1O2) for cancer cell destruction, a furan moiety for RNA crosslinking in the presence of 1O2, and polyethylenimine (PEI) for electrostatically binding to mitochondria. This design offers multiple benefits, including the absence of phototoxicity in sunlight since PDT is activated by near-infrared (NIR) light, significant cell destruction by targeting the energy-producing organelles-mitochondria, and disruption of cellular function through damage to cytoplasmic RNA. The findings show that the nanoplatform achieved remarkable cancer regression both in vitro and in vivo, outperforming non-targeting alternatives.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"537"},"PeriodicalIF":5.3,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144726402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Urea-mediated growth engineering of Au@Ag core-shell nanostructures: an enzymatic detection strategy with machine learning-assisted comparative analysis. 尿素介导的Au@Ag核壳纳米结构的生长工程：一种机器学习辅助比较分析的酶检测策略。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-29 DOI: 10.1007/s00604-025-07375-8

Farzaneh Shalileh, Morteza Hosseini, Mohammad Golbashy, Mehdi Dadmehr, Hossein Sabahi

{"title":"Urea-mediated growth engineering of Au@Ag core-shell nanostructures: an enzymatic detection strategy with machine learning-assisted comparative analysis.","authors":"Farzaneh Shalileh, Morteza Hosseini, Mohammad Golbashy, Mehdi Dadmehr, Hossein Sabahi","doi":"10.1007/s00604-025-07375-8","DOIUrl":"https://doi.org/10.1007/s00604-025-07375-8","url":null,"abstract":"A non-invasive, enzyme-based colorimetric biosensor was developed for urea detection in saliva, utilizing a growth-based method with Au@Ag core-shell nanostructures, including CTAB-coated gold nanoparticles (AuNPs) and CTAB-coated gold nanorods with short (SAuNRs) and high (HAuNRs) aspect ratios. The biosensing mechanism relies on urease-mediated hydrolysis of urea, which raises the pH and enhances the reduction capability of ascorbic acid, leading to the formation of a silver shell on the gold nanostructures, causing colorimetric changes correlated to urea concentration. A machine-learning comparative analysis was also performed to assess how the nanostructure morphology of AuNPs, SAuNRs, and HAuNRs affects sensor performance. The results showed that the biosensor effectively detected urea with all three nanostructures, achieving the highest precision and accuracy using HAuNRs, with a limit of detection (LOD) of 0.26 mM and a linear range of 0.5-50 mM urea. The best-performing machine learning algorithms, evaluated using R2, RMSE, and NRMSE metrics, were gradient boosting and extreme gradient boosting when applied to high aspect ratio gold nanorods. This innovative approach provides a cost-effective and user-friendly platform for urea detection, making it a promising tool for non-invasive diagnostics.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"538"},"PeriodicalIF":5.3,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144740801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0