Microchimica Acta最新文献

{"title":"Porous covalent architecture-involved luminescent smart hydrogel with target-responsive framework for signal-amplified inspection of aflatoxin B1","authors":"Tong Zhai,&nbsp;Yu-Di Shen,&nbsp;Miao Yu,&nbsp;Jing-Min Liu,&nbsp;Shuo Wang","doi":"10.1007/s00604-025-07145-6","DOIUrl":"10.1007/s00604-025-07145-6","url":null,"abstract":"<div><p>Robust detection techniques combined with functional micro-/nano-entities can be an ideal solution for food-safety risk treatment related to aflatoxin B1 (AFB1). In this work, a porous covalent architecture-involved luminescent smart hydrogel with dynamic cross-linking and target-responsive framework was prepared for specific determination of AFB1 with remarkable sensitivity. Different from the existing hydrogel probe, the introduction of covalent organic frameworks (COFs) enhanced the hydrophobicity of hydrogel, enabling the selective capture of AFB1 with improved affinity while excluding large matrix molecules, thus creating an anti-interference detection environment. Additionally, the pH-responsive carbon dots (CDs) embedded in the smart hydrogel contributed to a dual signal amplification, induced by deconstruction of hydrogel to release the CDs and urease-catalyzed hydrolysis reaction to increase the pH, when the AFB1 presented. In view of this signal amplification and trace enrichment strategy, this smart hydrogel can achieve robust determination of AFB1 with a detection limit of 0.03 µM as well as good specificity. The method was validated using real-world samples, demonstrating excellent accuracy and precision compared to standard HPLC methods. This work not only advances the field of AFB1 detection but also provides a versatile and cost-effective platform that can be adapted for the detection of other toxins and biomolecules, addressing global food safety and environmental monitoring challenges.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monolithic capillary electrochromatographic system based on gold nanoparticles and chitosan chiral molecularly imprinted polymers for enantioseparation of metolachlor and separation of structural analogs","authors":"Ziyun Bu,&nbsp;Qiankang Si,&nbsp;Xiaolin Lu,&nbsp;Pei Wang,&nbsp;Dezhen Wu,&nbsp;Yingying Mei","doi":"10.1007/s00604-025-07142-9","DOIUrl":"10.1007/s00604-025-07142-9","url":null,"abstract":"<div><p> A novel monolithic capillary column (AuNPs@CMIP(poly-Chitosan)@capillary) with hybridized chiral molecular imprinted polymers (CMIPs) as stationary phase was prepared using S-metolachlor (S-MET) as template, chitosan as functional monomer, glutaraldehyde as cross-linker, and aminated-gold nanoparticles (NH₂-AuNPs) as nanocarriers. The capillary electrochromatography (CEC) system constructed by AuNPs@CMIP(poly-Chitosan)@capillary achieved the efficient chiral separation of MET (resolution (Rs) = 9.83) as well as separation of its various structural analogs (Rs &gt; 1.58). In addition, AuNPs@CMIP(poly-Chitosan)@capillary exhibits satisfactory reproducibility and stability. The relative standard deviations (RSDs) of migration times and Rs for intra-day, inter-day, and inter-column were all less than 6.1%, and no significant decline in separation performance was observed after 21 days of storage. NH₂-AuNPs, with their large specific surface area and stable structure, can provide abundant binding sites and play a supporting role, reducing the deformation and collapse of CMIPs’ cavities, and further ensuring the structural stability and separation performance of the stationary phase. Finally, parameters such as imprinting factor and selectivity factor were calculated by adsorption experiments, and the CEC separation mechanism was discussed. The chitosan CMIPs-based capillary monolithic column developed in this work has rarely been reported and is an innovative attempt, providing a feasible and promising approach for the development of CEC chiral monolithic stationary phases.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovations in graphene-based electrochemical biosensors in healthcare applications","authors":"Sudenur Ozbey,&nbsp;Gulsu Keles,&nbsp;Sevinc Kurbanoglu","doi":"10.1007/s00604-025-07141-w","DOIUrl":"10.1007/s00604-025-07141-w","url":null,"abstract":"<div><p>The isolation of a single atomic layer of graphite, known as graphene, marked a fundamental moment that transformed the field of materials science. Graphene-based nanomaterials are recognized for their superior biocompatibility compared with many other types of nanomaterials. Moreover, one of the main reasons for the growing interest in graphene is its potential applications in emerging technologies. Its key characteristics, including high electrical conductivity, excellent intrinsic charge carrier mobility, optical transparency, substantial specific surface area, and remarkable mechanical flexibility, position it as an ideal candidate for applications in solar cells and touch screens. Its durability further establishes graphene as a strong contender for developing robust materials. To date, a variety of methods, such as traditional spectroscopic techniques and chromatographic approaches, have been developed for detecting biomolecules, drugs, and heavy metals. Electrochemical methods, known for their portability, selectivity, and impressive sensitivity, offer considerable convenience for both patients and professionals in point-of-care diagnostics. Recent advancements have significantly improved the capacity for rapid and accurate detection of analytes in trace amounts, providing substantial benefits in biosensor technology. Additionally, the integration of nanotechnology has markedly enhanced the sensitivity and selectivity of electrochemical sensors, yielding significantly improved results. Innovations such as point-of-care, lab-on-a-chip, implantable devices, and wearable sensors are discussed in this review.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07141-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural biomass–derived carbon quantum dots: a path to antioxidant, anticancer, antibiofilm, and bacterial bioimaging potential","authors":"Prakashkumar Nallasamy,&nbsp;Pavithra Kannan,&nbsp;Manickam Selvaraj,&nbsp;Mohammed A. Assiri,&nbsp;Suganthy Natarajan","doi":"10.1007/s00604-025-07132-x","DOIUrl":"10.1007/s00604-025-07132-x","url":null,"abstract":"<div><p>Eco-friendly, highly fluorescent, and biocompatible carbon quantum dots (CQDs) were synthesized from <i>Typha angustifolia</i> by the hydrothermal method. Fabricated CQDs were assessed for its bioimaging properties, anticancer potential, antibiofilm, and antioxidant activities. X-ray diffraction analysis indicates an amorphous nature, with an average particle size of 11 nm as observed in dynamic light scattering. TEM analysis revealed a uniform quasi-spherical-shaped structure. Photoluminescence studies reveal that CQDs exhibit an excitation at 390 nm with emissions at 484 nm and 474 nm. Water-soluble CQDs showed a potent antiproliferative effect against human breast cancer (MDA-MB-231) cell lines with an IC₅₀ value of 70.55 ± 0.015 µg/mL. Fluorescent studies revealed that CQDs enhanced the intracellular reactive oxygen species level leading to the loss of mitochondrial membrane potential triggering the apoptotic pathway culminating to cell death in MDA-MB-231 cells. In addition, CQDs showed a potent free radical scavenging activity with an IC₅₀ value of 21.1 ± 1.56 μg/mL exhibiting 94.69 ± 2.4% scavenging activity at the highest dose of 100 µg/mL. Furthermore, CQDs efficiently eradicated the biofilm-forming efficiency of <i>Pseudomonas aeruginosa</i> which frequently affects immunocompromised cancer patients. Cellular internalization studies in <i>P. aeruginosa</i> showed intense green fluorescence revealing the applicability of CQDs for bioimaging. Overall, the results indicate that CQDs fabricated from a natural source can serve as an excellent nanotheranostic and anti-infective agent for the treatment of triple-negative breast cancer.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoparticles-based electrochemical sensing platform for high-through immunoassay with redox-activity CaCO3 nanotags on a magnetic microfluidic device","authors":"Shaofeng Lin,&nbsp;Ting Lin,&nbsp;Haipeng Xu,&nbsp;Haining Li,&nbsp;Wenwen Zhang,&nbsp;Chenyu Wu,&nbsp;Shuyi Lu,&nbsp;Yanping Chen,&nbsp;Xiao Han","doi":"10.1007/s00604-025-07149-2","DOIUrl":"10.1007/s00604-025-07149-2","url":null,"abstract":"<div><p> A new nanoparticles-based sensing platform was designed for high-through electrochemical immunoassay of ferritin (FET) biomarker on a magneto-controlled microfluidic device by using anti-FET capture antibody-conjugated magnetic sensing probes. Thionine-doped calcium carbonate (CaCO₃) nanoparticles labeled with anti-FET detection antibodies were utilized as the recognition elements. Introduction of target FET caused the sandwich-type immunoreaction between two antibodies. The formed immunocomplexes were attached onto magnetic microfluidic sensing interface through an external magnet. Subsequently, the carried CaCO₃ nanoparticles were dissolved under acidic conditions to release the doped thionine molecules with redox activity. The thionine-based voltammetric signals increased with the increment of target FET levels within the linear range 0.01–100 ng mL⁻¹. The limit of detection was 7.9 pg mL⁻¹ FET. Good analytical properties such as selectivity, reproducibility, and accuracy were achieved with the nanoparticles-based magnetic electrochemical immunoassay. More significantly, the magnetic microfluidic electrochemical immunoassay provides new opportunities for rapid, simple, and cost-effective serum sample analysis.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Early and sensitive diagnosis of celiac autoimmune disease by using carboxylic acid functionalized magnetic nanoparticles-assisted biosensing platform","authors":"Elif Burcu Aydın,&nbsp;Muhammet Aydın,&nbsp;Mustafa Kemal Sezgintürk","doi":"10.1007/s00604-025-07129-6","DOIUrl":"10.1007/s00604-025-07129-6","url":null,"abstract":"<div><p>A novel impedimetric magneto-immunosensor based on iron oxide (Fe₃O₄) nanoparticles coated with 3-phosphonopropionic acid (3-PPA) (functionalized magnetic beads, or FMBs) was created for the highly sensitive and selective detection of anti-tissue transglutaminase antibody (anti-tTG) in human serum. This label-less immunosensor was introduced by magnetically attaching FMBs onto the working electrode surface with a neodium magnet. The FMBs were utilized as a sensing interface and had carboxylic acid groups for tTG molecules, which could selectively link the target anti-tTG antibody. The FMBs modification steps were carried out in microcentrifuge tubes and concentrated with magnetic force before electrochemical analyses. The specific immuno-interactions on the FMBs surface were characterized by using the electrochemical and microscopic techniques, and in the presence of anti-tTG antibodies, they were captured by tTG-immobilized magnetic beads, and significant increases were observed in impedimetric response. The magneto biosensor response was linearly related to the anti-tTG antibody level in a broad linear range of 0.125–15.62 U/mL and a low detection limit (LOD) of 0.04 U/mL. Additionally, this magneto sensor was stable, repeatable, reproducible, selective, and sensitive for determination of the anti-tTG. The commercial enzyme-linked immunosorbent assay (ELISA) method was employed to compare the responses of the suggested immunosensor in actual samples. The magneto biosensor results were in good agreement with the ELISA reference technique results. Consequently, the biosensor performance in the analysis of serum samples was acceptable.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07129-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A self-calibrated tri-emission fluorescent probe based on carbon dots assembly for in vitro pepsin detection","authors":"Lan Yu,&nbsp;Qinan Jiang,&nbsp;Xiao Yang,&nbsp;Fengyu Liu,&nbsp;Qi Zhang,&nbsp;Weiqiang Han,&nbsp;Guoxin Liu,&nbsp;Hongjuan Li,&nbsp;Yongqian Xu,&nbsp;Shiguo Sun","doi":"10.1007/s00604-025-07119-8","DOIUrl":"10.1007/s00604-025-07119-8","url":null,"abstract":"<div><p>A novel self-calibrating triple-emission fluorescent probe (CDs/BSA-SQ-Cy7) was engineered through the strategic integration of blue-emitting carbon dots (CDs) with covalently assembled near-infrared fluorescent dyes (BSA-SQ and BSA-SQ-Cy7) for accurate in vitro pepsin detection. To validate the enhanced performance of the tri-emissive design, a dual-emission control probe (CDs/BSA-SQ) was developed. BSA-SQ-Cy7 undergoes hydrolysis upon interaction with pepsin, resulting in concentration-dependent quenching of the red fluorescence, where the blue CDs serve as a reference emission during the process. The CDs/BSA-SQ-Cy7 probe demonstrated a robust linear correlation with pepsin concentrations ranging from 0.018 to 30 μg/mL (determined by the lowest detection limit). The detection limits were found to be 0.182 μg/mL (<i>I</i>₄₉₅/<i>I</i>₆₅₉), 0.033 μg/mL (<i>I</i>₄₉₅/<i>I</i>₈₀₈), and 0.018 μg/mL ((<i>I</i>₄₉₅-<i>I</i>₈₀₈)/<i>I</i>₆₅₉), respectively. Additionally, the probe displayed excellent stability and reproducibility over a 72-h period, and its three-channel emission signal ratios can be utilized for self-calibration, thereby improving assay accuracy. This self-calibrated tri-emission probe is distinguished by its stability, reproducibility and high selectivity, offering an effective method for the accurate in vitro detection of pepsin.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of metal oxide and metallic nanoparticles in indoor air samples using mixed cellulose esters filters and spICP-MS: dissolve and shoot","authors":"Carlos Gómez-Pertusa,&nbsp;M. Carmen García-Poyo,&nbsp;Guillermo Grindlay,&nbsp;Ricardo Pedraza,&nbsp;Adela Yañez,&nbsp;Luis Gras","doi":"10.1007/s00604-025-07139-4","DOIUrl":"10.1007/s00604-025-07139-4","url":null,"abstract":"<div><p>In recent years, there has been increasing concern about the adverse health effects of the metallic and metal-containing nanoparticles (NPs) present in indoor environments. Unfortunately, there is no well-stablish method to simultaneously characterize their number and composition. Recently, our research group proposed a strategy for the determination of metallic nanoparticles in air by means spICP-MS based on the aerosol collection on micro-quartz filters and the subsequent extraction using microwave heating in basic media. Although the proposed method allows accurate and precise characterization of NPs, it suffers from practical drawbacks: (i) micro-quartz filter fibers are released into the sample and must be removed prior to analysis to avoid clogging the nebulizer and (ii) the particle distribution detection limits (LOD_size) achieved are not low enough (28 nm). In this work, we evaluate the NPs trapping capabilities and possible fiber release of filters of different nature commonly used for indoor air quality control (polytetrafluoroethylene (PTFE), nylon, polycarbonate, and mixed cellulose ester (MCE) filters) and NPs of different chemical composition (ZrO₂-, TiO₂-, Pt-, AuNPs), size (20–150 nm), and capping agent (citrate, polyethylene glycol, branched polyethyleneimine, and lipoic acid). The results show that MCE is an optimal solution because it is completely dissolved during the microwave heating step and NPs are recovered quantitatively irrespective of their composition and size. The LODs are also improved down to 15 nm and 120 particles per liter of air, low enough to be used for indoor air pollution control. Finally, the proposed method was successfully tested in a simulated (NPs enriched) indoor environment.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07139-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sorbent magnetic levitation in the 3D printed platform","authors":"Olga Mokhodoeva,&nbsp;Valeriia Maksimova,&nbsp;Tatiana Danilova,&nbsp;Denis Trofimov,&nbsp;Artem Krivenko,&nbsp;Valery Shkinev","doi":"10.1007/s00604-025-07116-x","DOIUrl":"10.1007/s00604-025-07116-x","url":null,"abstract":"<div><p>The emerging technologies based on magnetic levitation and 3D printing manufacturing were utilized to create a sorbent retention platform as an alternative to traditional packed columns. The magnetic nanoscale sorbent was suspended in the aqueous solution in a 3D printed sorption cell placed between two permanent magnets. Simulations of hydrodynamic fluid and magnetic field were carried out to optimize the configuration and positioning of the sorption cell. The developed platform ensures uniform distribution of the sorbent in the cell’s volume and retention under dynamic conditions. The efficiency of palladium solid-phase extraction in a flow mode was demonstrated using modified magnetite nanoparticles. A high concentration factor can be achieved using the spiral shape of the cell with 50–100 mg of the suspended sorbent. The results of the spectrometric determination of trace amounts of palladium in natural water are given. An automated system for environmental analysis based on the proposed platform is promising. </p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transfer learning and data augmentation for glucose concentration prediction from colorimetric biosensor images","authors":"Ga-Young Choi,&nbsp;Na-Ri Kim,&nbsp;Da-Young Yu,&nbsp;Taeha Lee,&nbsp;Gyudo Lee,&nbsp;Han-Jeong Hwang","doi":"10.1007/s00604-025-07136-7","DOIUrl":"10.1007/s00604-025-07136-7","url":null,"abstract":"<div><p> A deep learning algorithm is introduced to accurately predict glucose concentrations using colorimetric paper sensor (CPS) images. We used an image dataset from CPS treated with five different glucose concentrations as input for deep learning models. Transfer learning was performed by modifying four established deep learning models—ResNet50, ResNet101, GoogLeNet, and VGG-19—to predict glucose concentrations. Additionally, we attempted to alleviate the challenge of requiring the large amount of training data by applying data augmentation techniques. Prediction performance was evaluated using coefficients of determination (<i>R</i>²), root mean squared error (RMSE), and relative-RMSE (rRMSE). GoogLeNet showed the highest coefficient of determination (<i>R</i>² = 0.994) and significantly lower prediction errors across all concentration levels compared with a traditional machine learning approach (two-sample <i>t</i>-test, <i>p</i> &lt; 0.001). When data augmentation was performed using 20% of the entire training dataset, the mean prediction error was comparable to that of the original entire training dataset. We presented a novel approach for glucose concentration prediction using deep learning techniques based on transfer learning and data augmentation with image data. Our method uses images from CPS as input and eliminates the need for separate feature extraction, simplifying the prediction process.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}