Microchimica Acta最新文献

{"title":"Confined active area and aggregation kinetic-based AuNPs@PVP nanosensors for simultaneous colorimetric detection of cysteine and homocysteine as homologues in human urine and serum.","authors":"Hua-Li Li, Jie-Ling Liang, Shun-Xing Li, Feng-Jiao Liu, Jia-Yi Luo, Zhao-Jing Huang, Feng-Ying Zheng, Ling-Ling Su, You-Bin Zhuo","doi":"10.1007/s00604-025-06962-z","DOIUrl":"https://doi.org/10.1007/s00604-025-06962-z","url":null,"abstract":"<p><p>The detection of cysteine (Cys) and homocysteine (Hcy) in biological fluids has great significance for early diagnosis, including Alzheimer's and Parkinson's disease. The simultaneous determination of Cys and Hcy with a single probe is still a huge challenge. To enlarge the differences in space structure (line and ring) and energy (-721.78 and -761.08 Hartree) between Cys and Hcy, and to cause a difference of aggregation kinetics, gold nanoparticles (AuNPs) are capped with hydrophilic and low-toxic polyvinylpyrrolidone (PVP) (named AuNPs@PVP) and some surface-active sites of AuNPs are masked, the active area for the binding between AuNPs and the detection object is confined, meanwhile, the stability of AuNPs is improved. A novel nanosensor based on confined active area and aggregation kinetics of AuNPs@PVP, is designed for the identification and determination of Cys and Hcy in 1 and 3 min, respectively, with sufficiently low detection limit (4.12 and 4.35 μM) and linear range (4.12-100 μM) for health evaluation. This single colorimetric sensor was applied successfully to the determination of urine and serum, evidencing high anti-interference ability.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":"108"},"PeriodicalIF":5.3,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143051229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical aptasensor for the selective detection of vancomycin based on nanostructured \"in-lab\" printed electrodes.","authors":"Malek Bibani, Magdolna Casian, Bogdan Feier, Diana Bogdan, Oana Hosu-Stancioiu, Nadia Ktari, Rafik Kalfat, Cecilia Cristea","doi":"10.1007/s00604-025-06952-1","DOIUrl":"10.1007/s00604-025-06952-1","url":null,"abstract":"<p><p>A label-free, flexible, and disposable aptasensor was designed for the rapid on-site detection of vancomycin (VAN) levels. The electrochemical sensor was based on lab-printed carbon electrodes (C-PE) enriched with cauliflower-shaped gold nanostructures (AuNSs), on which VAN-specific aptamers were immobilized as biorecognition elements and short-chain thiols as blocking agents. The AuNSs, characterized by scanning electron microscopy (SEM) and atomic force microscopy (AFM), enhanced the electrochemical properties of the platform and the aptamer immobilization active sites. The developed disposable aptasensor allowed label-free detection of VAN via electrochemical impedance spectroscopy (EIS) across a wide range of concentrations (50-1000 nM), with a limit of detection (LOD) of 1.721 nM. The aptasensor presented good selectivity against some commonly found interferences in human serum and milk and was successfully applied to the analysis of these samples.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":"107"},"PeriodicalIF":5.3,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11762413/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of CHS-Fe₃O₄@@ZIF-8 peroxidase-mimic with an ultra-thin hollow layer for ultrasensitive electrochemical detection of kanamycin.","authors":"Jiadong Zhang, Yaoying Cui, Yanchao Dou, Jingying Zhu, Xiangyu Ma, Liming Guo, Kai Zhang, Nan Hao, Liangdong Feng, Yi Chen","doi":"10.1007/s00604-025-06965-w","DOIUrl":"10.1007/s00604-025-06965-w","url":null,"abstract":"<p><p>A highly sensitive and selective electrochemical biosensor was developed for the detection of kanamycin using a core-hollow-shell structured peroxidase-mimic nanozyme, CHS-Fe₃O₄@@ZIF-8. The synthesized CHS-Fe₃O₄@@ZIF-8 was characterized with scanning electron microscopy, transmission electron microscopy, and X-ray photoelectron spectroscopy. It was found that the CHS-Fe₃O₄@@ZIF-8 exhibits excellent peroxidase-like activity due to  its ultra-thin hollow layer. Besides, CHS-Fe₃O₄@@ZIF-8 functionalized with complementary chains of kanamycin aptamer was anchored to the electrode surface via complementary base pairing with the kanamycin aptamer. Upon the presence of kanamycin, a strand displacement reaction was triggered leading to a reduction in the number of the CHS-Fe₃O₄@@ZIF-8, which slowed down the catalytic reaction of the substrate 3,3',5,5' -tetramethylbenzidine (TMB) facilitated by CHS-Fe₃O₄@@ZIF 8. Differential pulse voltammetry (DPV) was employed to measure and record changes in peak current resulting from catalytic oxidation product formation (oxidation product of TMB). The electrochemical signal exhibited a linear relationship with logarithmic variations in kanamycin concentration within a range spanning from 10 to 8000 pM and achieved an impressive detection limit as low as 7.52 pM. Furthermore, successful detection of kanamycin content in serum samples using this sensor demonstrated its good specificity and reproducibility. These findings indicate that the constructed electrochemical kanamycin sensor holds significant potential for practical applications. The biosensor demonstrated high selectivity, distinguishing kanamycin from other antibiotics, and exhibited good reproducibility, making it reliable for practical applications. The successful detection of kanamycin in serum samples further underscores the sensor's potential for real-world applications, particularly in monitoring antibiotic residues in food products and clinical diagnostics.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":"106"},"PeriodicalIF":5.3,"publicationDate":"2025-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aptamer-antibody sandwich immunosensor for electrochemical detection of FT4.","authors":"Yuechan Shi, Beibei Hu, Baoyuan Liu, Xiaomeng Li, Yongshuai Jing, Zhongqiu Li, Zhiwei Li, Shiguo Sun","doi":"10.1007/s00604-025-06951-2","DOIUrl":"10.1007/s00604-025-06951-2","url":null,"abstract":"<p><p>An aptamer-antibody sandwich electrochemical immunosensor was studied. Fe₃O₄/MWCNTs-COOH/Nafion was modified and fixed on a glassy carbon electrode to amplify electrical signals. The antibody was coupled with AuNPs to form conjugates. It captures the target free thyroxine (FT4) and forms antibody-AuNPs/FT4 macromolecular complexes. The complex is then captured by the aptamer on the glassy carbon electrode and detected by the electrochemical workstation using differential pulse voltammetry (DPV). Under the best conditions, the prepared sensor showed a linear relationship in the concentration range 0.1 to 10⁵ pg/mL and the detection limit (LOD) was 0.04 pg/mL. The sensor can enhance the adsorption capacity of target FT4 and detect FT4 stably and with high selectivity. The aptamers and antibodies are free from additional modifications. In addition, the high recovery of the proposed sensor was verified by testing the human serum samples. The immunosensor has the advantages of simple operation, rapid recognition process, and short response time. These results indicate that the immunosensor is expected to achieve rapid and effective detection of FT4 in early pregnancy and evaluate thyroid function in the future. The research ideas of this work will also be applicable to the detection of other small molecules and provide ideas for the detection of other substances.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":"105"},"PeriodicalIF":5.3,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bimetallic metal-organic frameworks as electrode modifiers for enhanced electrochemical sensing of chloramphenicol.","authors":"Jiazhen Tang, Jiawei Chen, Ruijie Xu, Junhui Xu, Xiaolun Peng, Yazhen Wang","doi":"10.1007/s00604-024-06930-z","DOIUrl":"https://doi.org/10.1007/s00604-024-06930-z","url":null,"abstract":"<p><p>An electrochemical sensor is presented for the detection of the chloramphenicol (CAP) based on a bimetallic MIL-101(Fe/Co) MOF electrocatalyst. The MIL-101(Fe/Co) was prepared by utilizing mixed-valence Fe (III) and Co (II) as metal nodes and terephthalic acid as ligands with a simple hydrothermal method and characterized by SEM, TEM, XRD, FTIR, and XPS. Electrochemical measurements such as electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and differential pulse voltammetry (DPV) showed that bimetallic MIL-101(Fe/Co) had the faster electron transfer, larger electroactive area, and higher electrocatalytic activity compared with  their monometallic counterparts due to the strong synergistic effect between bimetals. Inspired by these results, the MIL-101(Fe/Co)-based sensor was used to detect CAP. Some experiment parameters of pH, Fe and Co molar ratio, MIL-101(Fe/Co) volume, and DPV quiet time were optimized. The direct reduction mechanism of CAP was verified to involve four electrons and four protons process. Finally, the sensitive and selective CAP detection in the concentration range 1 to 200 μM with a detection limit of 0.3 μM was realized by the proposed sensor. The satisfactory recoveries in tap water and lake water indicated the practicability of the proposed electrochemical sensor. It is expected that this work may open up a paradigm for the preparation of MOF-based electrode modifiers with desired electrocatalytic performance for environmental pollution monitoring.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":"104"},"PeriodicalIF":5.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoenzyme-based sensors for the detection of anti-tumor drugs.","authors":"Xiaojing Zhang, Lina Zou, Hao Liao, Huanyu Ren, Huiru Niu, Zheng Li, Xueqing Zhang, Xiaojing Huang, Yanan Liu, Zhiren Zhou, Hongzhi Pan, Hongkun Ma, Shengzhong Rong","doi":"10.1007/s00604-024-06822-2","DOIUrl":"10.1007/s00604-024-06822-2","url":null,"abstract":"<p><p>Natural enzymes are a class of biological catalysts that can catalyze a specific substrate. Although natural enzymes have catalytic activity, they are susceptible to the influence of external environment such as temperature, and storage requirements are more stringent. Since the first discovery of magnetic Fe₃O₄ nanoparticles with peroxidase-like activity in 2007, the research on nanoenzymes has entered a rapid development stage. Nanoenzymes synthesized by chemical methods not only have the catalytic activity of natural enzymes but also are more stable, easy to store, and convenient to prepare. Anthracyclines, as a commonly used anti-tumor chemotherapy drug, will produce many side effects such as myelosuppression and liver function damage after long-term use, which will affect its therapeutic effects. This paper reviews the characteristics, classification, and mechanisms of nanoenzymes. The detection of anti-tumor drugs, especially anthracycline drugs, using a nanoenzyme-based sensor was emphatically introduced. On this basis, the application of nanoenzyme-based sensors in the detection of anti-tumor drugs is  prospected.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":"103"},"PeriodicalIF":5.3,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143021555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancing foodborne pathogen detection: a review of traditional and innovative optical and electrochemical biosensing approaches","authors":"Zeus Saldaña-Ahuactzi,&nbsp;Francisco Javier Gómez-Montaño,&nbsp;Janet Morales-Chávez,&nbsp;Rafael A. Salinas,&nbsp;Claudia Reyes-Betanzo,&nbsp;Marlon Rojas-López,&nbsp;Ateet Dutt,&nbsp;Abdú Orduña-Díaz","doi":"10.1007/s00604-024-06924-x","DOIUrl":"10.1007/s00604-024-06924-x","url":null,"abstract":"<div><p>Foodborne diseases are a significant cause of morbidity (600 million cases) and mortality (420,000 deaths) worldwide every year and are mainly associated with pathogens. Besides the direct effects on human health, they have relevant concerns related to financial, logistics, and infrastructure for the food and medical industries. The standard pathogen identification techniques usually require a sample enrichment step, plating, isolation, and biochemical tests. This process involves specific facilities, a long-time analysis procedures, and skilled personnel. Conversely, biosensors are an emerging innovative approach to detecting pathogens in real time due to their portability, specificity, sensitivity, and low fabrication costs. These advantages can be achieved from the synergistic work between nanotechnology, materials science, and biotechnology for coupling biomolecules in nano-matrices to enhance biosensing performance. This review highlights recent advancements in electrochemical and optical biosensing techniques for detecting bacteria and viruses. Key properties, such as detection limits, are examined, as they depend on factors like the design of the biorecognition molecule, the type of transducer, the target's characteristics, and matrix interferences. Sensitivity levels reported range from 1 to 1 × 10⁸ CFU/mL, with detection times spanning 10 min to 8 h. Additionally, the review explores innovative approaches, including biosensors capable of distinguishing between live and dead bacteria, multimodal sensing, and the simultaneous detection of multiple foodborne pathogens — emerging trends in biosensor development.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous or separate detection of heavy metal ions Hg2+ and Ag+ based on lateral flow assays","authors":"Zhili Wang,&nbsp;Yueyang Cheng,&nbsp;Ting Tang,&nbsp;Xiaoru Zhang,&nbsp;Xunyi Yuan","doi":"10.1007/s00604-025-06956-x","DOIUrl":"10.1007/s00604-025-06956-x","url":null,"abstract":"<div><p>A lateral flow assay (LFA) was developed for the simultaneous or separate detection of mercury ion and silver ion based on isothermal nucleic acid amplification. T-Hg²⁺-T and C–Ag⁺-C were utilized in the isothermal nucleic acid amplification strategy to form specific complementary base pairs. Under the action of KF polymerase and endonuclease Nt.BbvCl, trace amounts of Hg²⁺ and Ag⁺ were converted to Product-Hg²⁺ and Product-Ag⁺ as bridges. Biotin-labeled capture strands (Biotin-DNA1, Biotin-DNA1, and Biotin-DNA3) immobilized on the test strips could capture the Au NPs-DNA nanoprobes by hybridization with the generated bridge products for monitoring of two heavy metal ions simultaneously or separately. The assembly method of DNAs on the nanoprobes was explored, and the DNA sequences on the nanoprobes were designed so that only one kind of DNA strand was used to bind to all three capture DNA strands on the C, T1, and T2 bands. Under optimal detection conditions, the limits of detection for Hg²⁺ and Ag⁺ were 2.19 and 5.41 pM, respectively, with desired selectivity and reproducibility.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MAO-B-triggered reaction for an optical triple-signal assay and AND logic gate application based on PEI-functionalized silver nanoparticles","authors":"Xin-Ru Zhang,&nbsp;Tian Chai,&nbsp;Xiang-Lei Chang,&nbsp;Ying Zhang,&nbsp;Cheng-Bo Wang,&nbsp;Song Liu,&nbsp;Yves Iradukunda,&nbsp;Xian-Yi Shang,&nbsp;Yong-Hong Cao,&nbsp;Wei-Feng Wang,&nbsp;Jun-Li Yang,&nbsp;Yin Qiang","doi":"10.1007/s00604-024-06908-x","DOIUrl":"10.1007/s00604-024-06908-x","url":null,"abstract":"<div><p>A novel analytical method was designed and developed that exhibited ultraviolet–visible (UV–Vis), fluorescence (FL), and resonance Rayleigh scattering (RRS) signals for straightforward and comprehensive determination of monoamine oxidase B (MAO-B) using polyethylenimine-functionalized silver nanoparticles (PEI-Ag NPs). Through a facile one-step experiment, and NaOH assisted, in an aqueous solution of 100 ℃ for 40 min PEI reacted with AgNO₃ to generate PEI-Ag NPs with a yellow color and weak blue fluorescence. Interestingly, phenylacetaldehyde (PAA), a specific product of MAO-B, causes significant enhancement of the three optical signals of UV–Vis, FL, and RRS. A triple-signal readout sensing system was designed and constructed for MAO-B concentration assay. The limit of detection (LOD) was 0.42 (UV–Vis), 3.49 (FL), and 1.97 (RRS) <i>μ</i>g/mL. AND logic gate analysis was applied to enhance its accurate identification. Eventually, the feasibility of the method was assessed by measuring MAO-B amount in human serum samples with satisfying recoveries. The method provided a new concept for the clinical detection of MAO-B.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-based non-invasive detection of salivary uric acid based on the fluorescence quenching of gleditsia sinensis carbon dots","authors":"Yujuan Xiang,&nbsp;Hongpeng Yu,&nbsp;Kegang Wu,&nbsp;Ziting Quan,&nbsp;Dong He,&nbsp;Cansheng Ou,&nbsp;Xianghua Chai,&nbsp;Yinglin Liang,&nbsp;Xuejuan Duan,&nbsp;Xiangyu Zhang,&nbsp;Qin Zha,&nbsp;Wei Xie","doi":"10.1007/s00604-025-06949-w","DOIUrl":"10.1007/s00604-025-06949-w","url":null,"abstract":"<div><p>A smartphone-based non-invasive method was developed for salivary uric acid detection using <i>Gleditsia Sinensis</i> carbon dots (GS-CDs). The GS-CDs synthesized by the one-pot hydrothermal method emitted blue fluorescence at a maximum excitation wavelength of 350 nm and had good fluorescence stability in the presence of different ions, while showing selectivity to uric acid solution. The ability of uric acid (UA) to quench the fluorescent substances present in the GS-CDs, was confirmed through HPLC-FLD and LC–MS, FTIR and XPS. The results showed that UA reacted with GS-CDs, with a decrease in hydroxyl groups and the formation of carboxyl groups. The fluorescence quenching suggested a possible dynamic quenching mechanism. In addition, a smartphone-based non-invasive detection method was developed for the detection of salivary UA levels, which reflects blood UA levels. This study provides a new perspective on the utilization of GS shells and advances the development of non-invasive testing for UA.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}