Microchimica Acta最新文献

Improved mechanical stability of laser-induced graphene electrodes in enzyme-free glucose sensors through transferring to PDMS and partial PDMS etching. 通过转移到PDMS和部分PDMS蚀刻提高无酶葡萄糖传感器中激光诱导石墨烯电极的机械稳定性。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-08-02 DOI: 10.1007/s00604-025-07390-9

Sang Hyun Park, Taeheon Kim, James Jungho Pak

{"title":"Improved mechanical stability of laser-induced graphene electrodes in enzyme-free glucose sensors through transferring to PDMS and partial PDMS etching.","authors":"Sang Hyun Park, Taeheon Kim, James Jungho Pak","doi":"10.1007/s00604-025-07390-9","DOIUrl":"https://doi.org/10.1007/s00604-025-07390-9","url":null,"abstract":"The fabrication process and characteristics of flexible glucose sensors using laser-induced graphene (LIG) electrodes and transferring them onto a flexible polydimethylsiloxane (PDMS) film is described. The electrochemical glucose sensor consists of a working electrode, a counter electrode, and a reference electrode, all of which can be fabricated by irradiating a CO2 laser directly onto a polyimide (PI) film following the designed electrode pattern. This fabrication process allows simple formation of LIG electrode patterns on PI (LIG/PI). These LIG/PI electrodes are quite vulnerable, and hence, those electrodes were transferred onto a slightly melted polydimethylsiloxane (PDMS) and fixed to make LIG/PDMS electrodes with improved robustness. To demonstrate the durability improvement, the LIG/PI and LIG/PDMS electrodes underwent rubbing (once) and bending (100 times) tests for comparison, which shows a substantial improvement with the LIG/PDMS electrodes. An enzyme-free glucose sensor was then fabricated with the LIG/PDMS electrodes and by electroplating Cu onto the LIG/PDMS working electrode as the glucose-sensing electrode. Evaluation of the sensor's electrochemical performance revealed a sensitivity of 7.49 µA/mM·cm2.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"550"},"PeriodicalIF":5.3,"publicationDate":"2025-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanomaterial-enhanced biosensors for polycystic ovarian syndrome diagnosis and pathophysiological insights. 纳米材料增强生物传感器对多囊卵巢综合征的诊断和病理生理学的见解。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-08-01 DOI: 10.1007/s00604-025-07415-3

Bakr Ahmed Taha, Marwa Amin Al-Rawi, Ali J Addie, Sinan Adnan Abdulateef, Adawiya J Haider, Vishal Chaudhary, Norhana Arsad

{"title":"Nanomaterial-enhanced biosensors for polycystic ovarian syndrome diagnosis and pathophysiological insights.","authors":"Bakr Ahmed Taha, Marwa Amin Al-Rawi, Ali J Addie, Sinan Adnan Abdulateef, Adawiya J Haider, Vishal Chaudhary, Norhana Arsad","doi":"10.1007/s00604-025-07415-3","DOIUrl":"https://doi.org/10.1007/s00604-025-07415-3","url":null,"abstract":"Polycystic ovarian syndrome (PCOS) is an endocrine disease characterized by hormonal imbalances, metabolic inefficiency, and infertility problems. Furthermore, anti-Müllerian hormone (AMH), testosterone, and insulin are PCOS biomarkers that need to be detected accurately for early diagnosis and treatment. A narrative review discusses current improvements in nanomaterial-enhanced biosensors that detect biomarkers with high sensitivity and specificity. In addition, nanomaterials such as graphs and quantum dots have large surface areas and unique physicochemical properties that make them more effective biosphere. In addition, wearables and lab-on-chip platforms benefit from these features because they can detect in real time. In addition, artificial intelligence (AI) and machine learning (ML) are investigated to increase data interpretation and risk stratification using optical and electrochemical biosensors. Consequently, these biosensor technologies provide molecular insights into the underlying causes of PCOS, such as hyperandrogenism, insulin resistance, and chronic inflammation. By developing a short and portable biosensor, we can bridge the bridge between laboratory research and clinical practice and provide user-friendly diagnosis. Along with increasing clinical accuracy, the nanomaterial-based biosensor is considered a platform to learn more about PCOS pathology. Finally, their integration into clinical practice can contribute to the development of individual treatment methods in reproductive endocrinology and encourage research in the future.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"547"},"PeriodicalIF":5.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cobalt ferrite magnetic nanoparticles as stirring actuators to improve UV-Vis spectroelectrochemical measurements in normal reflection mode. 钴铁氧体磁性纳米颗粒作为搅拌致动器改善正常反射模式下紫外可见光谱电化学测量。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-08-01 DOI: 10.1007/s00604-025-07351-2

Alessandra Cutillo-Foraster, Nurhayat Özbek, Lluís Otero-de-Muller, Julio Bastos-Arrieta, Núria Serrano, José Manuel Díaz-Cruz

{"title":"Cobalt ferrite magnetic nanoparticles as stirring actuators to improve UV-Vis spectroelectrochemical measurements in normal reflection mode.","authors":"Alessandra Cutillo-Foraster, Nurhayat Özbek, Lluís Otero-de-Muller, Julio Bastos-Arrieta, Núria Serrano, José Manuel Díaz-Cruz","doi":"10.1007/s00604-025-07351-2","DOIUrl":"https://doi.org/10.1007/s00604-025-07351-2","url":null,"abstract":"Spectroelectrochemical (SEC) measurements using UV-Vis radiation in normal reflection mode (or normal configuration) are less sensitive than parallel approaches, since the volume of sample monitored has a much lower proportion of the diffusion layer created by the electrochemical processes, i.e., the region where relevant optical changes take place. In contrast, the normal configuration is more robust and reproducible and, as of today, is the only commercially available. This work presents a strategy to enhance normal reflection SEC measurements of Fe(III)/(II)-orthophenanthroline system using a screen-printed carbon electrode (SPCE), improving competitiveness with parallel designs. This method required the design of a new measuring cell based on the geometry of the commercial one, but replacing the eight magnets by a non-magnetic closing system. The developed approach involves adding cobalt ferrite magnetic nanoparticles (CoFe2O4 MNPs) to the analyte solution and coupling the SEC cell to a conventional magnetic stirrer. The resulting nanostirring, driven by MNPs movement, enhances mass transport toward the electrode. This accelerates diffusion layer renewal, leading to a noticeable increase of both electrochemical and optical signals.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"546"},"PeriodicalIF":5.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CuCrO₂/Cr₂O₃ heterojunction gas sensor for low-temperature and high response H₂S detection and its application for meat freshness evaluation. 低温高响应H2S检测的CuCrO2/Cr2O3异质结气体传感器及其在肉类新鲜度评价中的应用

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-08-01 DOI: 10.1007/s00604-025-07398-1

Baoyu Song, Jinghan Wang, Xiaona Zhang, Kuo Lin, Wenxiao Li, Fei Ye, Ying Fu, Yingming Xu

{"title":"CuCrO2/Cr2O3 heterojunction gas sensor for low-temperature and high response H2S detection and its application for meat freshness evaluation.","authors":"Baoyu Song, Jinghan Wang, Xiaona Zhang, Kuo Lin, Wenxiao Li, Fei Ye, Ying Fu, Yingming Xu","doi":"10.1007/s00604-025-07398-1","DOIUrl":"https://doi.org/10.1007/s00604-025-07398-1","url":null,"abstract":"Hydrogen sulfide (H2S) is a highly toxic and flammable gas that is also an important indicator of meat spoilage. Therefore, real-time and accurate detection of H2S concentration is essential to ensure environmental safety and human health. Despite its significance, achieving highly sensitive detection of H2S gases at low temperatures remains a formidable challenge. In this study, we used the biomass template method to controllably prepare CuCrO2-doped Cr2O3 p-p heterojunction hollow tubes. Among them, the 10.93 wt% Cu-doped Cr2O3 sensing material (Cu-Cr2O3-3) obtained by calcination at 500 °C has a good overall gas-sensing performance to H2S gas. It has a response value of up to 441.2 for 100 ppm H2S gas at 92 °C, as well as good selectivity, low calculated detection limit of 11.15 ppb and humidity resistance. Meanwhile, the reasons for the performance differences between the 5 composites were explored in detail by using various characterisation methods. The gas sensing mechanism of the Cu-Cr2O3-3 sensor was also analysed by combining MS, XPS and 'quasi-in-situ' Raman spectrum. In addition, we carried out experiments on the freshness detection of pork and shrimp by the Cu-Cr2O3-3 sensor, confirming its potential application in meat freshness evaluation.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"549"},"PeriodicalIF":5.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical bioplatform for the determination of the most common and carcinogenic dibutyl phthalate migrated from daily-use plastic products. 电化学生物平台用于测定从日用塑料制品中迁移的最常见和致癌的邻苯二甲酸二丁酯。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-08-01 DOI: 10.1007/s00604-025-07418-0

Elif Burcu Aydın, Muhammet Aydın, Mustafa Kemal Sezgintürk

{"title":"Electrochemical bioplatform for the determination of the most common and carcinogenic dibutyl phthalate migrated from daily-use plastic products.","authors":"Elif Burcu Aydın, Muhammet Aydın, Mustafa Kemal Sezgintürk","doi":"10.1007/s00604-025-07418-0","DOIUrl":"https://doi.org/10.1007/s00604-025-07418-0","url":null,"abstract":"Dibutyl phthalate (DBP) is a typical plasticizer used in the production and processing of plastic items. Given that DBP is easily leached and discharged into the environment, posing a threat to nature and living things, a facile and feasible impedimetric magneto biosensor for ultrasensitive determination of DBP was constructed first of all by using epoxy-functionalized magnetic nanoparticles as a sensing platform. A magnetic field on the electrode surface kept the epoxy-functionalized magnetic nanoparticles in place, and the particular interaction was conducted on the solid platform of magnetic nanoparticles. This sensing platform with a large surface area enhanced to attach a large amount of DBP-specific aptamer leads to a further increase in the sensitivity of the aptasensor. The DBP-specific aptamer was covalently attached to the epoxy groups of the 3-(glycidoxypropyl)trimethoxysilane (GPMS) agent standing at the magnetic nanoparticle surface without using any crosslinking agent. Meanwhile, the DBP aptamer attached to the as-prepared epoxy-functionalized magnetic nanoparticles via amide bonds specifically recognized the DBP analyte. The interactions of DBP with the aptamer on the epoxy-functionalized magnetic nanoparticles were studied by different electrochemical techniques, and for the quantification of DBP, electrochemical impedance spectroscopy (EIS) was utilized. In the best conditions, the electrochemical aptasensor showed good results for measuring DBP, with a broad range from 1 to 200 pg/mL and a very low detection limit (LOD) of 0.32 pg/mL. Importantly, the resulting aptasensor had excellent repeatability and reproducibility, desirable specificity, long storage stability, and high sensitivity, indicating its potential applications in food and environmental safety. In addition, the real sample experiments performed on the daily-use plastic products revealed the feasibility of the proposed detection approach.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"548"},"PeriodicalIF":5.3,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144764345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dual-channel fluorescence biosensor for simultaneous miR-133a and ATP detection in living muscle cells. 用于同时检测活肌细胞中miR-133a和ATP的双通道荧光生物传感器。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-31 DOI: 10.1007/s00604-025-07364-x

Liandong Zhao, Mingzhu Zhao, Jing Wang, Shuqi Zhang, Rong-Mei Kong, Jianxin Yang, Yan Zhao, Weiheng Kong

{"title":"Dual-channel fluorescence biosensor for simultaneous miR-133a and ATP detection in living muscle cells.","authors":"Liandong Zhao, Mingzhu Zhao, Jing Wang, Shuqi Zhang, Rong-Mei Kong, Jianxin Yang, Yan Zhao, Weiheng Kong","doi":"10.1007/s00604-025-07364-x","DOIUrl":"https://doi.org/10.1007/s00604-025-07364-x","url":null,"abstract":"Muscle injuries present a significant challenge in sports and physical activity, necessitating advanced diagnostic tools. Current invasive methods, such as muscle biopsies, are limited by procedural risks and lack of dynamic data. In this study, we developed a dual-channel fluorescent biosensor based on MoS2 nanosheets and fluorescence resonance energy transfer (FRET) for the simultaneous detection of miR-133a and ATP in living mouse myoblast cells. The design leverages the pathophysiological roles of miR-133a (a biomarker upregulated during muscle injury) and ATP (a critical indicator of energy metabolism). The biosensor incorporates FAM-labeled miR-133a-specific ssDNA and Cy5-labeled ATP aptamer probes immobilized on MoS2 nanosheets, achieving fluorescence quenching via FRET. Target binding triggered fluorescence recovery, enabling sensitive detection with limits of detection (LOD) of 3.3 pM for miR-133a and 0.33 µM for ATP. In vitro experiments confirmed high specificity and biocompatibility in mouse myoblasts, validated through confocal microscopy and flow cytometry. This platform provides a foundational tool for monitoring molecular dynamics associated with muscle injury, focusing on miR-133a and ATP levels in cellular environments. This work addresses limitations of traditional invasive diagnostics and lays the groundwork for potential applications in sports medicine research.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"543"},"PeriodicalIF":5.3,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Temperature-responsive AuNPs/poly(N-isopropylacrylamide) hydrogel-based sensor for SERS detection of paraquat in fruit juices. 温度响应型AuNPs/聚n -异丙基丙烯酰胺水凝胶传感器用于果汁中百草枯的SERS检测。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-31 DOI: 10.1007/s00604-025-07366-9

Jingyi Fan, Yiqun Huang, Junjian Miao, Keqiang Lai

{"title":"Temperature-responsive AuNPs/poly(N-isopropylacrylamide) hydrogel-based sensor for SERS detection of paraquat in fruit juices.","authors":"Jingyi Fan, Yiqun Huang, Junjian Miao, Keqiang Lai","doi":"10.1007/s00604-025-07366-9","DOIUrl":"https://doi.org/10.1007/s00604-025-07366-9","url":null,"abstract":"A novel composite SERS substrate was developed based on gold nanoparticles (AuNPs) and poly(N-isopropylacrylamide) (PNIPAM) hydrogel. PNIPAM hydrogel is a temperature-responsive material with a lower critical solution temperature (LCST) of about 32 °C, regulating its swelling-shrinking behavior by changing temperature to evaluate the enrichment efficiency of target molecules. Experimental results demonstrated that the substrate exhibited a swelling ratio of 400-600% at room temperature, facilitating the efficient enrichment of paraquat molecules. The optimal Raman response was observed when the substrate contained a NIPAM concentration of 0.8 mol/L and an AuNPs concentration of 300%. Additionally, the substrate exhibited excellent detection performance, including high sensitivity, good reproducibility, uniformity, and temporal stability. The minimum detectable concentration of paraquat in standard solutions was 0.1 μg/L. In apple juice and orange juice, the detection limit ranged from 0.072 to 0.112 μg/L, with spiked recoveries of 85.55%-110.55% and 88.57%-107.15%, respectively. This study presents a highly selective and reliable SERS sensing strategy for the rapid detection of trace paraquat in juice samples.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"542"},"PeriodicalIF":5.3,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of magneto-luminescent gold nanoclusters with microfluidic systems to the determination analysis of tetracyclines. 磁致发光金纳米团簇微流控系统在四环素类药物测定分析中的应用。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-31 DOI: 10.1007/s00604-025-07405-5

Javier Palma-Roldán, Vanesa Román-Pizarro, Miguel Ángel García-Granados, Juan Manuel Fernández-Romero, Ángela Écija-Arenas

{"title":"Application of magneto-luminescent gold nanoclusters with microfluidic systems to the determination analysis of tetracyclines.","authors":"Javier Palma-Roldán, Vanesa Román-Pizarro, Miguel Ángel García-Granados, Juan Manuel Fernández-Romero, Ángela Écija-Arenas","doi":"10.1007/s00604-025-07405-5","DOIUrl":"10.1007/s00604-025-07405-5","url":null,"abstract":"A microfluidic analytical system based on magnetically retained nanomaterials has been developed using fluorometric detection. This system utilizes magnetic gold nanoclusters (AuMNCs) to determine total tetracyclines using three of them as model-chlortetracycline (CTC), doxycycline (DC), and oxytetracycline (OTC)-in water samples by monitoring the change in the luminescent signal produced upon interaction. AuMNCs were synthesized by adding preformed magnetic nanoparticles (MNPs) to bovine serum albumin (BSA) as stabilizer and ascorbic acid as a reducing agent. Their magnetic properties enable their retention in the reaction/detection zone of the microfluidic system. The reactor was integrated into the optical pathway of a conventional spectrofluorometer with a 3D-printed device to align the excitation beam with the microchannel. The instrumental signal was obtained at a 22.5° angle from the excitation beam. Key parameters were studied, including pH (optimal at 11 with carbonate buffer), flow rate (40 µL·min⁻1), and AuMNCs injection volume (10 µL). The analytical signal was based on the fluorescence intensity ratio between the emission of the AuMNCs (650 nm) and the AuMNCs-tetracycline complexes (425 or 510 nm). Limits of detection of 0.41 µmol·L⁻1 for CTC, 0.70 µmol·L⁻1 for DC, and 0.61 µmol·L⁻1 for OTC, with relative standard deviations (RSD) below 6.2%, were achieved. Compared with non-retained AuMNCs, the magnetically retained system showed improved results. The system was applied to determine different tetracyclines in water samples, with recoveries in spiked river water samples from 86 to 111%. This approach offers a simple, label-free, and versatile alternative with potential for future development into multi-analyte sensing platforms or applications in complex matrices.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"545"},"PeriodicalIF":5.3,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12313782/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144758890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the total antioxidant capacity of Anchusa italica retz using carbon fiber microelectrode modified by Au-Ag alloy urchin-like nanostructures. 用金-银合金类海胆纳米结构修饰的碳纤维微电极评价海胆总抗氧化能力。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-31 DOI: 10.1007/s00604-025-07396-3

Han Cheng, Tao Fang, Yu Li, Uchkun Ishimov, Awais Ihsan, Yanbing Pan, Ruiting Tian, Jun Li, Syed Aftab Naqvi, Xianju Huang

{"title":"Evaluation of the total antioxidant capacity of Anchusa italica retz using carbon fiber microelectrode modified by Au-Ag alloy urchin-like nanostructures.","authors":"Han Cheng, Tao Fang, Yu Li, Uchkun Ishimov, Awais Ihsan, Yanbing Pan, Ruiting Tian, Jun Li, Syed Aftab Naqvi, Xianju Huang","doi":"10.1007/s00604-025-07396-3","DOIUrl":"https://doi.org/10.1007/s00604-025-07396-3","url":null,"abstract":"Anchusa italica Retz, a plant rich in bioactive compounds, has garnered considerable attention for its antioxidant properties and associated health benefits. However, the traditional methods for assessing antioxidant capacity often suffer from limited sensitivity and operational complexity. To overcome these challenges, gold-silver alloy urchin-like nanostructures (AAA-UNs) were synthesized using an ascorbic acid reduction method and electrodeposited onto a carbon fiber microelectrode (CFME) to form a highly sensitive electrochemical sensor (AAA-UNs/CFME). The morphology and structure of the synthesized material and modified electrode were characterized by scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Sensor performance was optimized using cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). The antioxidant capacity of Anchusa italica Retz was evaluated by monitoring changes in the electrochemical behavior of guanine (used as a model biomolecule) upon exposure to Fenton-generated hydroxyl radicals (·OH), with and without the protective presence of antioxidants. The AAA-UNs/CFME exhibited high sensitivity, stability, and anti-interference capability, achieving a detection limit of 0.05 mmol/L and a linear range of 0.3-3.0 mmol/L for guanine. Experimental results confirmed that Anchusa italica Retz extracts from different geographic origins possess significant antioxidant activity. Among these, the Xinjiang Anchusa italica Retz extract showed the highest radical scavenging efficiency (81.05%), followed by samples from Pakistan (69.63%) and Hainan (60.26%). These findings underscore the innovative application of the AAA-UNs/CFME sensor for reliable, rapid and sensitive assessment of antioxidant capacity in complex botanical samples.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"544"},"PeriodicalIF":5.3,"publicationDate":"2025-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly stable benzonitrile functionalized covalent organic framework coating for solid-phase microextraction of hydroxyphenyl esters in complex matrix samples. 高稳定的苯腈功能化共价有机骨架涂层用于复合基质样品固相微萃取羟基苯基酯。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-07-30 DOI: 10.1007/s00604-025-07419-z

Zong-Mu Dong, Liming Qin, Yaqing Guo, Miaoqing Xu, Jian-Feng Wu, Guang-Chao Zhao

{"title":"Highly stable benzonitrile functionalized covalent organic framework coating for solid-phase microextraction of hydroxyphenyl esters in complex matrix samples.","authors":"Zong-Mu Dong, Liming Qin, Yaqing Guo, Miaoqing Xu, Jian-Feng Wu, Guang-Chao Zhao","doi":"10.1007/s00604-025-07419-z","DOIUrl":"https://doi.org/10.1007/s00604-025-07419-z","url":null,"abstract":"A mesoporous surface benzonitrile functionalized covalent organic framework (TpBD-CN-COF) was synthesized as a novel solid-phase microextraction (SPME) fiber coating for the extraction of six hydroxyphenyl esters (PBs), and combined with gas chromatography-flame electric-ion detector (GC-FID). PBs were sensitively determined in aqueous solution. Due to its high specific surface area, high chemical stability in both strongly acidic and alkaline media, and in polar or non-polar media, as well as its high thermal stability, the TpBD-CN-COF fiber coating showed excellent extraction performances for PBs with an enrichment factor twice as high as that of TpBD-COF. Some parameters that influenced the extraction performance, such as extraction time and temperature, pH, and stirring speed were investigated. Under optimal conditions, a wide linear range (0.06-500 μg L-1), low limits of detection (LOD, 0.003-0.042 μg L-1) were obtained for PBs determination. The relative standard deviations (RSDs, n = 6) for intra-day, inter-day, and fiber-to-fiber were less than 8.06%, 7.8%, and 8.6%, respectively. Furthermore, the analytical method could be successfully used to detect six PBs in cosmetics and environmental water samples, achieving satisfactory recoveries ranging from 85.14 to 107.67%.","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 8","pages":"540"},"PeriodicalIF":5.3,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144752039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0