Microchimica Acta最新文献_第10页

Development of photoelectrochemical immunosensor based on halide perovskite protected by organometallic compounds for determining interleukin-17A (IL-17A) 开发基于有机金属化合物保护的卤化物过氧化物的光电化学免疫传感器，用于测定白细胞介素-17A (IL-17A)

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-14 DOI: 10.1007/s00604-024-06694-6

Xiaoqing Li, Feng Huang, Chengqi Bao, Rong Shao, Lei Deng, Minghui Yang

{"title":"Development of photoelectrochemical immunosensor based on halide perovskite protected by organometallic compounds for determining interleukin-17A (IL-17A)","authors":"Xiaoqing Li, Feng Huang, Chengqi Bao, Rong Shao, Lei Deng, Minghui Yang","doi":"10.1007/s00604-024-06694-6","DOIUrl":"10.1007/s00604-024-06694-6","url":null,"abstract":"<div><p>The overexpression of interleukin-17A (IL-17A) is closely associated with the pathogenesis of autoimmune diseases and cancer, rendering precise identification of IL-17A level critical for disease diagnosis and prognosis monitoring. In this study, CsPbBr<sub>3</sub> nanoclusters (NCs) were embedded in C<sub>16</sub>H<sub>14</sub>Br<sub>2</sub>O<sub>6</sub>Pb<sub>2</sub> organometallic compound (Pb-MA MOC) via a hot injection approach. Through this way, the issue of CsPbBr<sub>3</sub> NCs susceptible to decomposition in water was solved, and the photocurrent intensity that is generated by CsPbBr<sub>3</sub> was significantly enhanced. A highly sensitive photoelectrochemical (PEC) sensor for detecting IL-17A in human serum was developed using CsPbBr<sub>3</sub>/Pb-MA as the photoactive material. The electrode was initially modified with CsPbBr<sub>3</sub>/Pb-MA. Then, antibody-modified Fe<sub>3</sub>O<sub>4</sub> magnetic nanoparticles (MNs) with target analyte IL-17A captured, and IL-17A antibody-modified Au@CuNi diatomic catalyst (DAC) formed sandwich immune complex structure on the electrode. The existence of CuNi DAC led to a substantial reduction in photoelectric signal intensity due to oxidation of ascorbic acid in the supporting electrolyte. The photocurrent intensity exhibited linear correlation with IL-17A concentration within the range 15-750 pg/mL, and achieving an impressive detection limit of 1 pg/mL. Moreover, the sensor was successfully applied to the determination of IL-17A in human serum, suggesting its potential clinical applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142431044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioreceptors’ immobilization by hydrogen bonding interactions and differential pulse voltammetry for completely label-free electrochemical biosensors 通过氢键相互作用和差分脉冲伏安法固定生物受体，实现完全无标记的电化学生物传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-14 DOI: 10.1007/s00604-024-06738-x

Adaris López-Marzo, Marta Mas-Torrent

{"title":"Bioreceptors’ immobilization by hydrogen bonding interactions and differential pulse voltammetry for completely label-free electrochemical biosensors","authors":"Adaris López-Marzo, Marta Mas-Torrent","doi":"10.1007/s00604-024-06738-x","DOIUrl":"10.1007/s00604-024-06738-x","url":null,"abstract":"<div><p>Label-free electrochemical biosensors show great potential for the development of point-of-care devices (POCDs) for environmental and clinical applications. These sensors operate with shorter analysis times and are more economic than the labelled ones. Here, four completely label-free biosensors without electron transfer mediators were developed for hepatitis B virus (HBV) detection. The approach consisted in (i) the modification of gold surfaces with cysteamine (CT) or cysteine (CS) linkers, (ii) the subsequent antibody (Ab) immobilization, either directly by hydrogen bonding (HB) interactions or by covalent bonds (CB) using additional reagents, and (iii) measuring the biosensor response by electrochemical impedance spectroscopy (EIS) and differential pulse voltammetry (DPV). The electrode surfaces at each stage of the modification process were characterised by X-ray photo-electron spectroscopy (XPS) and atomic force microscopy (AFM). The combination of Ab immobilization by HB with the DPV analysis displayed improved repeatability, lower interference to serum matrix and similar limits of detection and quantification than the traditional biosensors that immobilize the Ab via CB and use EIS as readout technique. The Ab immobilization by HB is shown as a simple, efficient and low-cost alternative to CB ones, while DPV was faster and showed better performance than EIS. The CT-HB biosensor displayed the lowest limits of detection and quantification of 0.14 and 0.46 ng/mL, respectively, a 0.46–12.5 ng/mL linear analytical range, and 100% of recovery for 1/10 human serum media during HBV surface antigen detection by DPV. Even, it preserved the initial sensing capability after 7 days of its fabrication.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06738-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142434947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly sensitive time-resolved fluorescent microspheres lateral flow immunoassay for the quantitative detection of triadimefon and its metabolite residues in fruits and vegetables 用于定量检测水果和蔬菜中三唑酮及其代谢物残留的高灵敏度时间分辨荧光微球横向流动免疫分析法

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-14 DOI: 10.1007/s00604-024-06755-w

San-Jun Shi, Mei-Qi Ji, Rong-Fu Huang, Zi-Yan Fan

{"title":"Highly sensitive time-resolved fluorescent microspheres lateral flow immunoassay for the quantitative detection of triadimefon and its metabolite residues in fruits and vegetables","authors":"San-Jun Shi, Mei-Qi Ji, Rong-Fu Huang, Zi-Yan Fan","doi":"10.1007/s00604-024-06755-w","DOIUrl":"10.1007/s00604-024-06755-w","url":null,"abstract":"<div><p> A general one-step lateral flow immunochromatographic assay (LFIA) for the quantitative detection of triadimefon (TDF) and triadimenol (TDN) in fruit and vegetable samples was developed using time-resolved fluorescence microspheres (TRFM) as labels. A specific anti-triadimefon monoclonal antibody (mAb) was conjugated with TRFM to fabricate LFIA test strips. A time-resolved fluorometer as an LFIA reader was applied to obtain quantitative results and assess risk ranges for the LFIA test strips. Under the optimized experimental conditions, the limits of detection (LODs) in buffer/cucumbers/tomatoes/oranges were 0.046 ng/mL, 0.135 µg/kg, 1.047 µg/kg, and 5.811 µg/kg, respectively, which are <i>ca</i>. 1000 times lower than that of colloidal gold-labeled strips. The recovery in cucumber/tomato/orange samples was 109.4–116.7%, 87.7–110.9%, and 88.0–111.9%, respectively, indicating that the test strips had good reliability. Coupled with the easily customizable pretreatment procedures for various samples, the LFIA results were obtained within 18 min without the need for professional personnel or complicated equipment. TRFM-LFIA for TDF and TDN also shows remarkable specificity and precision. The test strips were also low-cost, portable, and convenient to use. These results indicate the test strips could be utilized as a novel strategy for on-site detection of TDF and TDN, which has the potential to expand and detect other pesticide or insecticide residues in food.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142434738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemiluminescence biosensor for MMP-2 determination using CRISPR/Cas13a and EXPAR amplification: a novel approach for anti-aging research 利用 CRISPR/Cas13a 和 EXPAR 扩增技术测定 MMP-2 的电化学发光生物传感器：抗衰老研究的新方法

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-14 DOI: 10.1007/s00604-024-06707-4

Qiang Tang, Jie Wang, Jiayi Zhang, Hongyu Zeng, Zhixue Su, Xiying Zhu, Jihua Wei, Yuanxun Gong, Qianli Tang, Kai Zhang, Xianjiu Liao

{"title":"Electrochemiluminescence biosensor for MMP-2 determination using CRISPR/Cas13a and EXPAR amplification: a novel approach for anti-aging research","authors":"Qiang Tang, Jie Wang, Jiayi Zhang, Hongyu Zeng, Zhixue Su, Xiying Zhu, Jihua Wei, Yuanxun Gong, Qianli Tang, Kai Zhang, Xianjiu Liao","doi":"10.1007/s00604-024-06707-4","DOIUrl":"10.1007/s00604-024-06707-4","url":null,"abstract":"<div><p>Matrix metalloproteinase-2 (MMP-2) plays a pivotal role in anti-aging research. Developing advanced detection platforms for MMP-2 with high specificity, sensitivity, and accessibility is crucial. This study introduces a novel electrochemiluminescence (ECL) biosensor for MMP-2 determination, leveraging the CRISPR/Cas13a system and Exponential Amplification Reaction (EXPAR). The biosensor operates by utilizing the T7 RNA polymerase to transcribe RNA from a DNA template upon MMP-2 interaction. This RNA activates Cas13a, leading to signal amplification and ECL detection. The incorporation of the \"photoswitch\" molecule [Ru(phen)<sub>2</sub>dppz]<sup>2+</sup> streamlines the process by eliminating the need for extensive electrode modification and cleaning. Under optimized conditions, the biosensor achieved an impressive detection limit of 12.8 aM for MMP-2. The platform demonstrated excellent selectivity, reproducibility, and stability, making it highly suitable for detecting MMP-2 in complex biological samples. This innovative approach shows great potential for applications in molecular diagnostics and anti-aging research.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142430990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An easily fabricated nanoporous Au membrane in drug detection with reusable functionality and high SERS performance 用于药物检测的纳米多孔金膜易于制造，具有可重复使用的功能和较高的 SERS 性能

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-12 DOI: 10.1007/s00604-024-06756-9

Yansheng Liu, Yukun Sun, Xiaobo Jia, Jin Zhou, Kang Li, Zhaoxu Li, Guofu Wang

{"title":"An easily fabricated nanoporous Au membrane in drug detection with reusable functionality and high SERS performance","authors":"Yansheng Liu, Yukun Sun, Xiaobo Jia, Jin Zhou, Kang Li, Zhaoxu Li, Guofu Wang","doi":"10.1007/s00604-024-06756-9","DOIUrl":"10.1007/s00604-024-06756-9","url":null,"abstract":"<div><p>A method for detecting methamphetamine (MET), ketamine (KET), and morphine (MOP) molecules is presented using a reusable substrate based on SERS. The SERS substrate was prepared by etching the Au/Ag alloy film to synthesize a nanoporous Au membrane (AuNPM). By optimizing the preparation conditions and using rhodamine 6G (R6G) as an analyte, the AuNPM exhibited good SERS performance with a limit of detection (LOD) of 10<sup>−9</sup> mol L<sup>−1</sup>. A competitive immunoassay category has been applied to the detection of MET, KET, and MOP. The MET, KET, and MOP antigens were functionalized on the surface of the AuNPM to specifically bind to the related drug antibodies. The Au nanoparticles (AuNPs) modified with 4-mercaptobenzoic acid (4-MBA) and antibodies against MET, KET, and MOP were used as nanotags. The 4-MBA served as the reporting molecule and drug antibodies were used to bind to free drug molecules in the target solution. The mixture of nanotags and target solution was dropped onto the antigen-modified AuNPM (antigen/AuNPM), and the free nanotags bind to the antigen/AuNPM. By comparing the SERS intensity of 4-MBA with the presence or absence of drug molecules, the drugs were qualitatively and quantitatively identified. Through this category, the LODs for detecting MET, KET, and MOP were 0.1, 1, and 1 ng mL<sup>−1</sup>, respectively. This study proposes an effective method for constructing SERS-based detection of drug molecules with good potential for practical applications.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142431003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Parkinson biomarker determination with an Au microflower–enhanced electrochemical immunosensor using non-Faradaic capacitance measurements 使用非法拉第电容测量法的金微花增强电化学免疫传感器测定帕金森生物标记物

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-11 DOI: 10.1007/s00604-024-06747-w

Luiz Otávio Orzari, Laís Canniatti Brazaca, Bruno Campos Janegitz

{"title":"Parkinson biomarker determination with an Au microflower–enhanced electrochemical immunosensor using non-Faradaic capacitance measurements","authors":"Luiz Otávio Orzari, Laís Canniatti Brazaca, Bruno Campos Janegitz","doi":"10.1007/s00604-024-06747-w","DOIUrl":"10.1007/s00604-024-06747-w","url":null,"abstract":"<div><p>This work comprehends the development and characterization of a carbon black-based electrode modified with Au microflowers to increase its effect as a capacitance biosensor for the determination of PARK7/DJ-1. Due to its high surface-to-volume ratio and biocompatibility, Au particles are suitable for antibody binding, and by monitoring surface capacitance, it is possible to identify the immune-pair interaction. Au microflowers allowed the adequate immobilization of Parkinsonian-related proteins: PARK7/DJ-1 and its antibody. The protein is associated with several antioxidant mechanisms, but its abnormal concentrations or mutations can be the cause of the loss of dopaminergic neurons, leading to Parkinson’s disease. The device was characterized by scanning electron microscopy and cyclic voltammetry, revealing the flower-like structures and the electrochemically-interest enhancements they provide, such as increased heterogeneous electron transfer rate coefficient and electroactive area. The self-assembled monolayers of different molecules were optimized with the aid of 2<sup>2</sup> central composite experiments and a linear calibration curve was obtained between 0.700 and 120 ng mL<sup>-1</sup> of PARK7/DJ-1, with a limit of detection of 0.207 ng mL<sup>-1</sup>. The data confirms that the addition of Au microflowers enhanced the electrochemical signal of the device, as well as allowed for the determination of an early stage Parkinson’s disease biomarker with appreciable analytical performance.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An epitope imprinted electrochemical sensor for highly sensitive and selective determination of prostate-specific antigen 用于高灵敏度和选择性测定前列腺特异性抗原的表位印迹电化学传感器。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-10 DOI: 10.1007/s00604-024-06737-y

Huikai Shao, Lingguo Zhao, Zhenping Liu, Jinqi Li

{"title":"An epitope imprinted electrochemical sensor for highly sensitive and selective determination of prostate-specific antigen","authors":"Huikai Shao, Lingguo Zhao, Zhenping Liu, Jinqi Li","doi":"10.1007/s00604-024-06737-y","DOIUrl":"10.1007/s00604-024-06737-y","url":null,"abstract":"<div><p> A simple method for highly selective and sensitive prostate-specific antigen (PSA) detection using a molecularly imprinted electrochemical sensor is presented. The sensor was developed through an epitope imprinted strategy combined with electrochemical measurement techniques. An epitope molecularly imprinted polymer (EMIP) film was constructed on a AuNPs-coated gold electrode surface through electropolymerization, utilizing the C-terminus epitope of PSA (KWIKDTIVANP) as the template molecular and <i>o</i>-phenylenediamine as the functional monomer. The characteristics of EMIP film were investigated by using a scanning electron microscope and electrochemical test methods, including electrochemical impedance spectroscopy and cyclic voltammetry. Key parameters such as electropolymerization cycles, elution and rebinding times, and the molar ratio of template molecular to functional monomer were systematically optimized. The sensor demonstrated a detection limit (LOD) of 0.31 fg/mL and exhibited an excellent linear response towards PSA concentration ranging from 1.0 fg/mL to 0.1 µg/mL. Furthermore, the EMIP sensor showed excellent selectivity against other biological macromolecules, such as bovine serum albumin, human serum albumin, alpha-fetoprotein, and carcinoembryonic antigen. With recoveries between 95.89 and 106.04% for PSA detection in human serums the EMIP/AuNPs/AuE electrochemical sensor showed great potential in real sample analysis.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical immunosensor based on PtNPs/MoS2@rGO composite for the detection of alpha-fetoprotein in human serum 基于 PtNPs/MoS2@rGO 复合材料的电化学免疫传感器用于检测人血清中的甲胎蛋白。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-10 DOI: 10.1007/s00604-024-06712-7

Shiyu Zhang, Xin Chen, Shuai Hu, Ke Cai, Chenxi Peng, Lixia Luo, Yingying Gu, Yong Mei

{"title":"Electrochemical immunosensor based on PtNPs/MoS2@rGO composite for the detection of alpha-fetoprotein in human serum","authors":"Shiyu Zhang, Xin Chen, Shuai Hu, Ke Cai, Chenxi Peng, Lixia Luo, Yingying Gu, Yong Mei","doi":"10.1007/s00604-024-06712-7","DOIUrl":"10.1007/s00604-024-06712-7","url":null,"abstract":"<div><p>An electrochemical biosensor was created to identify the liver cancer marker alpha-fetoprotein (AFP) by employing nanocomposite materials. A combination of reduced graphene oxide (rGO) and molybdenum disulfide (MoS<sub>2</sub>) was selected as the substrate material for the sensor to prepare the PtNPs/MoS<sub>2</sub>@rGO electrochemical immunosensor. Among them, rGO has strong conductivity and MoS<sub>2</sub> provides a large surface area for the anchoring of PtNPs for better attachment to the hybridized nanomaterials. Meanwhile, PtNPs exhibit consistent biocompatibility and excellent electrocatalytic activity. PtNPs also attach to hybrid nanomaterials and bind the antibody via the Pt–S bond, thereby furnishing the antibody with multiple binding sites for enhanced antibody adhesion. The immunosensor achieved ultra-sensitive AFP detection by exploiting the specific antigen–antibody binding. The structure and morphology of the PtNPs/MoS<sub>2</sub>@rGO composites were investigated by transmission electron microscopy (TEM), energy dispersive X-ray (EDS) spectroscopy, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy, and the sensor was electrochemically characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Under optimized conditions, using differential pulse voltammetry the biosensor detected AFP in serum within a linear range of 1 ~ 10<sup>5</sup> pg/mL, with a correlation coefficient (<i>r</i><sup>2</sup>) of 0.9989, and a detection limit of 0.12 pg/mL (<i>S</i>/<i>N</i> = 3). The method offers a new approach for the ultrasensitive detection of serum AFP and is extremely selective, accurate, and precise with a relative standard deviation (RSD) of less than 6%. It has been successfully applied to the analysis of real human blood samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ratiometric fluorescence probe based on boric acid-modified carbon dots and alizarin red for sensitive and rapid detection of glyphosate 基于硼酸修饰碳点和茜素红的比率荧光探针，用于灵敏快速地检测草甘膦。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-10 DOI: 10.1007/s00604-024-06748-9

Jingqi Yang, Xinru Wang, Ying Yu, Yujuan Cao, Manli Guo, Xiaogang Hu, Li Wang, Bixia Lin

{"title":"Ratiometric fluorescence probe based on boric acid-modified carbon dots and alizarin red for sensitive and rapid detection of glyphosate","authors":"Jingqi Yang, Xinru Wang, Ying Yu, Yujuan Cao, Manli Guo, Xiaogang Hu, Li Wang, Bixia Lin","doi":"10.1007/s00604-024-06748-9","DOIUrl":"10.1007/s00604-024-06748-9","url":null,"abstract":"<div><p>By combining boric acid-modified carbon dots (<i>p</i>-CDs) and alizarin red (ARS), a double emission probe <i>p</i>-CDs@ARS with fluorescence at 410 nm and 600 nm is designed for the detection of glyphosate. When Cu<sup>2+</sup> is added, it binds with ARS to cause ARS release from <i>p</i>-CDs@ARS, which decreases the fluorescence at 600 nm. However, in the presence of glyphosate, glyphosate competes to the binding of Cu<sup>2+</sup>, releasing ARS to bind with <i>p</i>-CDs again. Therefore, the fluorescence of 600 nm recovers. Based on this, the fluorescence of 410 nm and 600 nm act as the reference and response signal, respectively, achieving the ratiometric fluorescence detection of glyphosate. The linear range of glyphosate detection is 0.5–50 µM with a limit of detection at 0.37 µM which is well below the maximum residue limit for glyphosate in food. When the probe is used to detect the glyphosate residue in Pearl River water and cucumber, the detection results are well consistent with those detected by HPLC. The established method based on <i>p</i>-CDs@ARS has the advantages that the assembly of ratiometric fluorescence probe is simple, and the detection speed is fast. Additionally, a typical INHIBIT logical system has been successfully constructed based on glyphosate, Cu<sup>2+</sup>, and the fluorescence signal of <i>p</i>-CDs@ARS.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142399041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in surface plasmon resonance for the detection of ovarian cancer biomarkers: a thorough review 表面等离子体共振用于检测卵巢癌生物标记物的最新进展：全面回顾。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-09 DOI: 10.1007/s00604-024-06740-3

Shahnam Valizadeh Shahbazlou, Somayeh Vandghanooni, Bahareh Dabirmanesh, Morteza Eskandani, Sadegh Hasannia

{"title":"Recent advances in surface plasmon resonance for the detection of ovarian cancer biomarkers: a thorough review","authors":"Shahnam Valizadeh Shahbazlou, Somayeh Vandghanooni, Bahareh Dabirmanesh, Morteza Eskandani, Sadegh Hasannia","doi":"10.1007/s00604-024-06740-3","DOIUrl":"10.1007/s00604-024-06740-3","url":null,"abstract":"<div><p>Early detection of ovarian cancer (OC) is crucial for effective management and treatment, as well as reducing mortality rates. However, the current diagnostic methods for OC are time-consuming and have low accuracy. Surface plasmon resonance (SPR) biosensors offer a promising alternative to conventional techniques, as they enable rapid and less invasive screening of various circulating indicators. These biosensors are widely used for biomolecular interaction analysis and detecting tumor markers, and they are currently being investigated as a rapid diagnostic tool for early-stage cancer detection. Our main focus is on the fundamental concepts and performance characteristics of SPR biosensors. We also discuss the latest advancements in SPR biosensors that enhance their sensitivity and enable high-throughput quantification of OC biomarkers, including CA125, HE4, CEA, and CA19-9. Finally, we address the future challenges that need to be overcome to advance SPR biosensors from research to clinical applications. The ultimate goal is to facilitate the translation of SPR biosensors into routine clinical practice for the early detection and management of OC.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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