Microchimica Acta最新文献_第10页

Iron/cobalt co-doped boron quantum dots as nanozymes with peroxidase-like activities and the nanozyme-involved cascade catalysis system for ratiometric fluorescence and dual-mode visual detection of glutamate 铁/钴共掺杂硼量子点作为具有过氧化物酶样活性的纳米酶及用于谷氨酸比例荧光和双模视觉检测的纳米酶级联催化系统

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-05-06 DOI: 10.1007/s00604-025-07183-0

Meng Yang, Hui Jin, Rijun Gui

{"title":"Iron/cobalt co-doped boron quantum dots as nanozymes with peroxidase-like activities and the nanozyme-involved cascade catalysis system for ratiometric fluorescence and dual-mode visual detection of glutamate","authors":"Meng Yang, Hui Jin, Rijun Gui","doi":"10.1007/s00604-025-07183-0","DOIUrl":"10.1007/s00604-025-07183-0","url":null,"abstract":"<div><p>To further explore the boron-involved nanomaterials toward efficient applications in chemo/bio sensing and detection fields, this work reports facile preparation of the emerging iron/cobalt co-doped boron quantum dots (Fe/Co@BQDs) that were explored as new artificial nanozymes for ratiometric fluorescence (FL) and visual detection of glutamate (Glu). In the presence of glutamate oxidase (GLOD), Glu was oxidized to produce H<sub>2</sub>O<sub>2</sub>, and then the H<sub>2</sub>O<sub>2</sub> was catalyzed by Fe/Co@ BQDs nanozymes to produce hydroxyl radical (•OH). Afterwards, the •OH induced FL quenching responses of rhodamine B (RhB) and Fe/Co@BQDs. Therefore, a new nanozyme-assisted cascade catalysis platform was explored, consisting of Fe/Co@BQDs, GLOD, and RhB. The platform was successfully used for ratiometric FL sensing of Glu and liquid/solid dual-channel FL visual semi-quantitative detection of Glu. The platform exhibits a board linear detection range of 1–500 µM, a low limit of detection of 0.3 µM, highly selective ratiometric FL responses on Glu over potential interferents, and high-performance practical detection of Glu in biological samples. Experimental results verify high peroxidase-like activities of Fe/Co@BQDs that enable efficient applications for unique enzymatic reactions and nanozyme-involved cascade catalysis reactions. The platform can facilitate further development of other types of metal-doped nanomaterials with natural biological enzyme-like activities and their promising applications, especially chemo/bio sensing, bioimaging and therapeutics at the levels of living cells and small animals.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143913821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biosensors based on organic transistors for intraoral biomarker detection 基于有机晶体管的口腔内生物标志物检测生物传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-05-05 DOI: 10.1007/s00604-025-07189-8

Ruotong Mai, Yixin Zhou, Kangning Zhao, Miao Xie, Yufei Tang, Xingrui Li, Wei Huang, Lin Xiang

{"title":"Biosensors based on organic transistors for intraoral biomarker detection","authors":"Ruotong Mai, Yixin Zhou, Kangning Zhao, Miao Xie, Yufei Tang, Xingrui Li, Wei Huang, Lin Xiang","doi":"10.1007/s00604-025-07189-8","DOIUrl":"10.1007/s00604-025-07189-8","url":null,"abstract":"<div><p>Intraoral biomarkers are important indicators for the diagnosis and prediction of oral and systemic diseases. Among various intraoral biomarkers, the biomarkers in saliva have been the main focus of research, due to their abundance, non-invasiveness, and correlation with health status. Nonetheless, detecting low-abundance intraoral biomarkers poses significant challenges, and the conventional assays are unsuitable for swift large-scale analysis due to their complex procedures. Hence, an immediate demand arises for innovative methods to supplant traditional assay techniques. Organic transistor–based biosensors have emerged as promising devices for the detection of these intraoral biomarkers, especially in point-of-care (POC) settings. These biosensors offer advantages such as high sensitivity, selectivity, ease of integration, and biocompatibility. This review provides an overview of the evolution and utilization of biosensors that rely on functional organic transistors, with a focus on electrolyte-gated organic field-effect transistors (EGOFETs) and organic electrochemical transistors (OECTs). First, the working principles and sensing mechanisms of various organic transistors are summarized. Then, recent progress and challenges in developing organic transistor–based biosensing platforms for detecting intraoral biomarkers are summarized, along with examples from representative studies. Last, prospects and opportunities for the advancement of organic transistor–based biosensors for oral health monitoring are discussed.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143908658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence-based spectrometric and imaging methods and machine learning analyses for microbiota analysis 基于荧光的光谱和成像方法以及用于微生物群分析的机器学习分析

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-05-05 DOI: 10.1007/s00604-025-07159-0

Jocelyn Reynolds, Jeong-Yeol Yoon

{"title":"Fluorescence-based spectrometric and imaging methods and machine learning analyses for microbiota analysis","authors":"Jocelyn Reynolds, Jeong-Yeol Yoon","doi":"10.1007/s00604-025-07159-0","DOIUrl":"10.1007/s00604-025-07159-0","url":null,"abstract":"<div><p>Most microbiota determination (skin, gut, soil, etc.) are currently conducted in a laboratory using expensive equipment and lengthy procedures, including culture-dependent methods, nucleic acid amplifications (including quantitative PCR), DNA microarray, immunoassays, 16S rRNA sequencing, shotgun metagenomics, and sophisticated mass spectrometric methods. In situ and rapid analysis methods are desirable for fast turnaround time and low assay cost. Fluorescence identification of bacteria and their mixtures is emerging to meet this demand, thanks to the recent development in various machine learning methods. High-dimensional spectroscopic or microscopic imaging data can be obtained to identify the bacterial makeup and its implications for human health and the environment. For example, we can classify healthy versus non-healthy skin microbiome, inflammatory versus non-inflammatory gut microbiome, degraded versus non-degraded soil microbiome, etc. This tutorial summarizes the various machine-learning algorithms used in bacteria identification and microbiota determinations. It also summarizes the various fluorescence spectroscopic methods used to identify bacteria and their mixtures, including fluorescence lifetime spectroscopy, fluorescence resonance energy transfer (FRET), and synchronous fluorescence (SF) spectroscopy. Finally, various fluorescence microscopic imaging methods were summarized that have been used to identify bacteria and their mixtures, including epi-fluorescence microscopy, confocal microscopy, two-photon/multi-photon microscopy, and super-resolution imaging methods (STED, SIM, PALM, and STORM). Finally, it discusses how these methods can be applied to microbiota determinations, what can be demonstrated in the future, opportunities and challenges, and future directions. </p></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 6","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143904861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of fluorescent sensor array based on internal self-polymerizing imprinted Janus nanosheets and its application in determination of multiple metal ions and bisphenol A 内部自聚合印迹Janus纳米片荧光传感器阵列的制备及其在多种金属离子和双酚A测定中的应用

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-05-02 DOI: 10.1007/s00604-025-07188-9

Shuyi Huang, Youai Ye, Xinglin Pang, Yuan Wang, Rixin Tan, Teyu Wu, Hanjie Li, Zhijun Bai, Yong Liang

{"title":"Preparation of fluorescent sensor array based on internal self-polymerizing imprinted Janus nanosheets and its application in determination of multiple metal ions and bisphenol A","authors":"Shuyi Huang, Youai Ye, Xinglin Pang, Yuan Wang, Rixin Tan, Teyu Wu, Hanjie Li, Zhijun Bai, Yong Liang","doi":"10.1007/s00604-025-07188-9","DOIUrl":"10.1007/s00604-025-07188-9","url":null,"abstract":"<div><p>Internal self-imprinting is an ingenious way to achieve the combination of imprinting and Pickering emulsions. Herein, internal self-polymerizing imprinted Janus nanosheets for detecting bisphenol A (BPA) and multiple metal ions are reported. Janus composite hollow spheres were prepared by grafting an imprinted polymer onto vinyl groups after an emulsion interfacial self-organized sol–gel process. After crushing, the external surface is further modified with thiol groups (-SH) and fluorescence signal indicators. Janus silica nanosheets (J-MIPs/SH@QDs) with bispecific artificial receptors (-SH and molecularly imprinted sites) were designed to specifically identify BPA and 4 metal ions (Hg<sup>2+</sup>, Cu<sup>2+</sup>, Cr<sup>3+</sup>, Ag<sup>+</sup>). CdTe QDs with red and yellow emissions were incorporated into the J-MIPs/SH@QDs as fluorescent signal indicators. Due to the presence of molecularly imprinted sites, BPA can be recognized with high specificity, resulting in increased fluorescence intensity. The thiol groups and metal ions formed a chelated structure with the fluorescence intensity decreasing. Linear discriminant analysis (LDA) in SPSS software can be used to analyze these specific fluorescence responses to distinguish BPA and multiple metal ions. In conclusion, J-MIPs/SH@QDs exhibit sensitivity in multi-organic–inorganic analyte combinations. Its desegregation of innovative bispecific receptors creates a multitude of opportunities for the specific and effective detection of coexisting contaminants.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of trace bisphenols in milk based on AMA/DVB@GO@UiO-66-NH2 dispersive micro solid-phase extraction coupled with HPLC–UV AMA/DVB@GO@UiO-66-NH2分散微固相萃取- HPLC-UV法测定牛奶中微量双酚类物质

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-05-02 DOI: 10.1007/s00604-025-07192-z

Weixia Li, Jiacheng Lei, Tiancheng He, Yaping Gan, Yan Zhu

{"title":"Determination of trace bisphenols in milk based on AMA/DVB@GO@UiO-66-NH2 dispersive micro solid-phase extraction coupled with HPLC–UV","authors":"Weixia Li, Jiacheng Lei, Tiancheng He, Yaping Gan, Yan Zhu","doi":"10.1007/s00604-025-07192-z","DOIUrl":"10.1007/s00604-025-07192-z","url":null,"abstract":"<div><p>A novel Zr-based metal–organic framework (MOF) functionalized with graphene oxide-coated allylmethacrylate-divinylbenzene microspheres (AMA/DVB@GO), termed AMA/DVB@GO@UiO-66-NH<sub>2</sub> was designed. The composite exhibits great adsorption capacity and selectivity for bisphenols (BPs), driven by synergistic interactions, including hydrophobic, π-π, and hydrogen bonding. We optimized conditions to establish an efficient dispersive micro-solid phase extraction procedure coupled with high-performance liquid chromatography (d-μSPE-HPLC) for extracting BPs from milk samples, using the AMA/DVB@GO@UiO-66-NH<sub>2</sub> composite. The method achieved recoveries ranging from 84.0 to 106.6%, with relative standard deviations below 6.9% and detection limits between 0.17 and 0.67 μg/L. This study introduced a novel, hydrophilic, and stable AMA/DVB@GO@UiO-66-NH<sub>2</sub> composite, offering a promising material for the selective enrichment and detection of BPs in food analysis.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143896637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancement in early diagnosis of polycystic ovary syndrome: biomarker-driven innovative diagnostic sensor 多囊卵巢综合征早期诊断的进展：生物标志物驱动的创新诊断传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-05-01 DOI: 10.1007/s00604-025-07187-w

Aniket Nandi, Kamal Singh, Kalicharan Sharma

{"title":"Advancement in early diagnosis of polycystic ovary syndrome: biomarker-driven innovative diagnostic sensor","authors":"Aniket Nandi, Kamal Singh, Kalicharan Sharma","doi":"10.1007/s00604-025-07187-w","DOIUrl":"10.1007/s00604-025-07187-w","url":null,"abstract":"<div><p>Polycystic ovary syndrome (PCOS) is a heterogeneous multifactorial endocrine disorder that affects one in five women around the globe. The pathology suggests a strong polygenic and epigenetic correlation, along with hormonal and metabolic dysfunction, but the exact etiology is still a mystery. The current diagnosis is mostly based on Rotterdam criteria, which resulted in a delayed diagnosis in most of the cases, leading to unbearable lifestyle complications and infertility. PCOS is not new; thus, constant efforts are made in the field of biomarker discovery and advanced diagnostic techniques. A plethora of research has enabled the identification of promising PCOS diagnostic biomarkers across hormonal, metabolic, genetic, and epigenetic domains. Not only biomarker identification, but the utilization of biosensing platforms also renders effective point-of-care diagnostic devices. Artificial intelligence also shows its power in modifying existing image-based analysis, even developing symptom-based prediction systems for the early diagnosis of this multifaceted disorder. This approach could affect the future management and treatment direction of PCOS, decreasing its severity and improving the reproductive life of women. The rationale of the current review is to identify the advancements in understanding the pathophysiology through biomarker discovery and the implementation of modern analytical techniques for the early diagnosis of PCOS.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Controlling fluid flow rate to separate leukocytes and cancer cells based on stiffness differences 根据硬度差异控制流体流速以分离白细胞和癌细胞

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-05-01 DOI: 10.1007/s00604-025-07186-x

Gaolin Li, Huan Liu, Shiyu Wang, Enxia Mao, Xuye Zhuang

{"title":"Controlling fluid flow rate to separate leukocytes and cancer cells based on stiffness differences","authors":"Gaolin Li, Huan Liu, Shiyu Wang, Enxia Mao, Xuye Zhuang","doi":"10.1007/s00604-025-07186-x","DOIUrl":"10.1007/s00604-025-07186-x","url":null,"abstract":"<div><p>Microfluidic techniques for label-free sorting of circulating tumor cells (CTCs) from peripheral blood are generally based on physical characteristic differences between blood cells and cancer cells, especially size differences. However, because the diameter ranges of some leukocytes and CTCs overlap, size-based sorting can certainly be disturbed by leukocytes. This study proposes a microfluidic sorting strategy based on cell stiffness differences, which achieves efficient separation of cells with overlapping sizes by precisely controlling the fluid flow rate. A quantitative relationship model between critical inlet flow rate, cell diameter, and Young’s modulus was established based on the cell deformation effect, and its accuracy was verified through fluid dynamics simulation and experiments. The results showed that when the flow rate was between the critical flow rates of leukocytes and CTCs, high-stiffness leukocytes could be captured by the slit, while low-stiffness CTCs passed smoothly, with a separation resolution of 0.3 µm. This method can serve as a supplement to existing size-based sorting techniques, significantly improving the purity of CTCs.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D printed electrode-microwell system: a novel electrochemical platform for miRNA detection 3D打印电极微孔系统：一种新型的miRNA检测电化学平台

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-05-01 DOI: 10.1007/s00604-025-07190-1

Panagiota M. Kalligosfyri, Chloe Miller, Stefano Cinti, Bhavik Anil Patel

{"title":"3D printed electrode-microwell system: a novel electrochemical platform for miRNA detection","authors":"Panagiota M. Kalligosfyri, Chloe Miller, Stefano Cinti, Bhavik Anil Patel","doi":"10.1007/s00604-025-07190-1","DOIUrl":"10.1007/s00604-025-07190-1","url":null,"abstract":"<div><p>3D printing has enabled the ability to make creative electrochemical well designs suitable for a wide field of electrochemical sensing. The demand for robust electrochemical systems is particularly high in diagnostics, where the rapid detection of emerging biomarkers associated with severe diseases is critical for rapid medical decision-making. This study is aimed at developing a fully 3D-printed electrochemical sensing device featuring a three-electrode system fabricated from conductive printing materials and incorporating a microwell as the sensing platform. The assay principle of a robust electrochemical screen-printed sensor was adapted for this platform, incorporating a well-structured design to enhance fluid control. This structure ensured the uniform distribution of reagents across the sensing surface, improving the reproducibility and consistency of measurements and enabling the reliable detection of a microRNA target associated with lung cancer. The detection process was based on the hybridization of the target miRNA with an immobilized DNA probe labeled with methylene blue as a redox mediator. The sensor was thoroughly characterized and optimized, achieving a dynamic detection range of 0.001 to 400 nM and a lower limit of detection compared to screen-printed sensors, down to the picomolar level. Furthermore, the sensor demonstrated high selectivity for the target miRNA compared to other miRNA sequences, proving its specificity. These results highlighted the potential of 3D printing technology for the development of sensitive and selective tools for biomarker detection, making it a valuable complementary method in the field of diagnostics.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07190-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chemiluminescent lateral flow assay based on transparent nitrocellulose membrane 基于透明硝化纤维素膜的化学发光横向流动试验

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-30 DOI: 10.1007/s00604-025-07106-z

Weimin Xu, Zixuan Ren, Li Yang, Yue Cui

{"title":"Chemiluminescent lateral flow assay based on transparent nitrocellulose membrane","authors":"Weimin Xu, Zixuan Ren, Li Yang, Yue Cui","doi":"10.1007/s00604-025-07106-z","DOIUrl":"10.1007/s00604-025-07106-z","url":null,"abstract":"<div><p>Lateral flow assays (LFA) have become increasingly important for point-of-care disease detection for low costs, portability, and rapid results. To improve chemiluminescence LFA (CL-LFA) performance, we present a way to make test (T) and control (C) line areas transparent by applying paraffin liquid to the nitrocellulose (NC) membrane without inducing structure deterioration. The transparent T and C line regions generate stronger light signals in CL-LFA compared with conventional LFA, where the NC membrane obstructs signals from inner conjugates. A tunable transparency ranging from 12.47% to 78.53% was achieved by increasing the paraffin volume and hence, reducing the light scattering among pores. Then, coating with 0.1 μL of paraffin liquid on the T and C line areas leads to a slower light attenuation constant of -1.35 lower than that of NC membrane (-1.72) when detecting 20 ng/mL of cardiac troponin I (cTnI), a key biomarker for myocardial injury. We achieved a detection limit of 0.063 ng/mL and clinical samples with cTnI levels ranging from 1 to 5.1 ng/mL were effectively distinguished, showing the validity and practicality of the device. This work offers fundamental insights into the materials used in LFA and contributes to the broader application of paper-based biosensors.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143892640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metal/heteroatom-doped carbon-based hybrid material as the matrix for rapid and high-throughput identification of phthalate esters in complex samples by laser desorption/ionization 金属/杂原子掺杂碳基杂化材料作为基体，用于激光解吸/电离快速高通量鉴定复杂样品中的邻苯二甲酸酯

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-29 DOI: 10.1007/s00604-025-07124-x

Luyan Meng, Baichun Wang, Yijia Rong, Shun Zhang, Ting Cai, Chuan-Fan Ding, Yinghua Yan

{"title":"Metal/heteroatom-doped carbon-based hybrid material as the matrix for rapid and high-throughput identification of phthalate esters in complex samples by laser desorption/ionization","authors":"Luyan Meng, Baichun Wang, Yijia Rong, Shun Zhang, Ting Cai, Chuan-Fan Ding, Yinghua Yan","doi":"10.1007/s00604-025-07124-x","DOIUrl":"10.1007/s00604-025-07124-x","url":null,"abstract":"<div><p>A novel matrix of metal particles and metal oxides modified N-doped multi-walled carbon nanotubes (N-MWCNTs/AuNPs/Co<sub>3</sub>O<sub>4</sub>) is introduced for the rapid detection of phthalate esters (PAEs) using surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS). By leveraging the synergistic effects of metal/heteroatom-doped carbon-based components, this innovative hybrid material demonstrated exceptional ionization efficiency for PAEs. Specifically, it enables the detection of bis(2-ethylhexyl) phthalate (DEHP) at concentrations as low as of 1 µg·mL⁻<sup>1</sup>. Moreover, the N-MWCNTs/AuNPs/Co<sub>3</sub>O<sub>4</sub> composite not only surpassed conventional organic matrices in terms of ionization efficiency and reproducibility, but also facilitated the effective enrichment of PAEs, achieving an enrichment efficiency range from 94.1% ± 5.8% to 98.2% ± 2.3%. Furthermore, this method was successfully applied to detect DEHP in human serum and urine samples, with urinary DEHP concentrations estimated ranging from 29.2 to 38.4 ng·mL⁻<sup>1</sup>. This approach represents a promising strategy for high-throughput PAE detection, offering significant potential for applications in environmental and biomedical analysis.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143883711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0