Microchimica Acta最新文献

{"title":"Nanoplasmonic microarray–based solid-phase amplification for highly sensitive and multiplexed molecular diagnostics: application for detecting SARS-CoV-2","authors":"Ji Young Lee,&nbsp;Hyowon Jang,&nbsp;Sunjoo Kim,&nbsp;Taejoon Kang,&nbsp;Sung-Gyu Park,&nbsp;Min-Young Lee","doi":"10.1007/s00604-024-06723-4","DOIUrl":"10.1007/s00604-024-06723-4","url":null,"abstract":"<div><p> A novel approach is introduced using nanoplasmonic microarray–based solid-phase recombinase polymerase amplification (RPA) that offers high sensitivity and multiplexing capabilities for gene detection. Nanoplasmonic microarrays were developed through one-step immobilization of streptavidin/biotin primers and fine-tuning the amplicon size to achieve high plasmon-enhanced fluorescence (PEF) on the nanoplasmonic substrate, thereby improving sensitivity. The specificity and sensitivity of solid-phase RPA on nanoplasmonic microarrays was evaluated in detecting E, N, and RdRP genes of SARS-CoV-2. High specificity was achieved by minimizing primer-dimer formation and employing a stringent washing process and high sensitivity obtained with a limit of detection of four copies per reaction within 30 min. In clinical testing with nasopharyngeal swab samples (<i>n</i> = 30), the nanoplasmonic microarrays demonstrated a 100% consistency with the PCR results for detecting SARS-CoV-2, including differentiation of Omicron mutations BA.1 and BA.2. This approach overcomes the sensitivity issue of solid-phase amplification, as well as offers rapidity, high multiplexing capabilities, and simplified equipment by using isothermal reaction, making it a valuable tool for on-site molecular diagnostics.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06723-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142540614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural optimization, characterization, and evaluation of binding mechanism of aptamers against bovine pregnancy-associated glycoproteins and their application in establishment of a colorimetric aptasensor using Fe-based metal-organic framework as peroxidase mimic tags","authors":"Chunxia Lu,&nbsp;Jiaxiang Qin,&nbsp;Shijia Wu,&nbsp;ZhenLiang Zhang,&nbsp;Zonggui Tang,&nbsp;Changbin Liu","doi":"10.1007/s00604-024-06775-6","DOIUrl":"10.1007/s00604-024-06775-6","url":null,"abstract":"<div><p> A truncated aptamer (designated A24-3) was identified that specifically binds to bovine pregnancy-associated glycoproteins (bPAG9) with a low dissociation constant (2.04 nM) through two truncation approaches. Circular dichroism spectroscopy indicated that A24-3 formed parallel G-quadruplexes, which was subsequently confirmed using nuclear magnetic resonance (NMR) spectroscopy. Furthermore, a molecular dynamics simulation was employed to investigate the recognition mechanism of A24-3 and bPAG9. Interaction analysis showed that A24-3 folded into a parallel G-quadruplex structure with three G-tetrads, primarily through numerous hydrogen bonds and hydrophobic and π-π interactions. Finally, a novel colorimetric aptasensor was developed for detecting bPAG9 using A24-3 and an Fe-based metal–organic framework as target recognition elements and enzyme mimics, respectively. The method demonstrated a broad detection range from 0.5 to 50 ng/mL, with a low detection limit of 0.03 ng mL⁻¹, and exhibited a good recovery (91.0–102%) for bPAG9-spiked serum samples. Additionally, the aptasensor was successfully applied to detecting the pregnancy-specific biomarker bPAGs in serum samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Branched CuAu nano-alloy for N2H4 oxidation-assisted H2 production and nitrite detection in water solution","authors":"Xingwang Sun,&nbsp;Xinmei Liu,&nbsp;Wenglong Yang,&nbsp;Liping Zhang","doi":"10.1007/s00604-024-06792-5","DOIUrl":"10.1007/s00604-024-06792-5","url":null,"abstract":"<div><p>The H₂ production using N₂H₄ splitting (OHzS) was often constrained by the requirement for insufficient stability, distinct catalysts at the anode and cathode, and the high-cost electrocatalyst associated with confined activity. This work verified the efficacy of surfactant-free branched CuAu nano-alloy as a bifunctional electrocatalyst for H₂ production. Benefiting from its favorable electronic structure and surfactant-free surface, surfactant-free CuAu nano-alloy demonstrated a reduced over-potential compared with pure Cu, pure Au, and CuAu nano-alloy prepared by surfactant. When using branched CuAu nano-alloy as both cathodic and anodic electrodes, a cell voltage of 0.768 V was required to drive a current density of 10 mA/cm². After 2550 min of H₂ generation, the amplitude of the working potential for anodic reactions was found to be less than 0.92%. The enhanced electrocatalytic activity could be also applied to H₂O₂ and NaNO₂ sensors. The CuAu nano-alloy exhibited a 2.35-folds increase in sensitivity compared to pure Au nano-crystals in the detection of H₂O₂. Moreover, the detection of NaNO₂ in water solution has been successfully achieved. The detection range 0–175.0 mM was much wider than that of sensors in previous works.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fabrication of a high-sensitivity electrochemical immunosensor by the oriented immobilization of engineered nanobody on nanofibrous membrane","authors":"Peng Wen,&nbsp;Hao Su,&nbsp;Wen-jia Yin,&nbsp;Jian-cheng Hu,&nbsp;Yu Wang,&nbsp;Jin-yi Yang,&nbsp;Zhi-li Xiao,&nbsp;Zhen-lin Xu,&nbsp;Yu-dong Shen,&nbsp;Hong Wang,&nbsp;Bruce D. Hammock","doi":"10.1007/s00604-024-06763-w","DOIUrl":"10.1007/s00604-024-06763-w","url":null,"abstract":"<div><p>A new type of label-free electrochemical immunosensor for the high-sensitivity determination of parathion was developed based on the oriented immobilization of nanobody (VHH9) on a gold nanoparticle-loaded polyvinyl alcohol/citric acid nanofiber membrane-modified electrode. The morphology characterization and assembly process of the modified materials were investigated using scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). Under the optimum conditions, the label-free electrochemical immunosensor for parathion exhibited a linear range of 0.0015–6400 ng/mL and a low detection limit of 0.48 pg/mL, the signal response of which was 10 times higher than that of the randomly immobilized VHH9. The immunosensor possessed high selectivity, good repeatability and reusability (keeping above 90% of its initial activity after repeating 8 times), and stability (remaining 90% after 9 weeks of storage). Finally, the average recoveries of parathion from food samples were 93.76–105.73% with the coefficient of variation being 2.65–6.85%, showing good correlation with UPLC (<i>R</i>² = 0.9950). Therefore, our nanobody immobilization protocol is simple and effective and proves the potential to be utilized as a promising candidate for sensing platform.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tuning the properties of peptide imprinted nanoparticles for protein immunoprecipitation using magnetic streptavidin beads","authors":"Ainhoa Elejaga-Jimeno,&nbsp;Alberto Gómez-Caballero,&nbsp;Gontzal García del Caño,&nbsp;Nora Unceta,&nbsp;Miquel Saumell-Esnaola,&nbsp;Joan Sallés,&nbsp;M. Aránzazu Goicolea,&nbsp;Ramón J. Barrio","doi":"10.1007/s00604-024-06782-7","DOIUrl":"10.1007/s00604-024-06782-7","url":null,"abstract":"<div><p> Maximizing the binding properties of thermoresponsive molecularly imprinted nanoparticles (MIN) was aimed to explore their feasibility as antibody substitutes in protein immunoprecipitation (IPP) with magnetic streptavidin beads (MSB). Thermoresponsive MIN targeting the cannabinoid CB₁ receptor were produced by epitope imprinting through solid-phase synthesis. It was intended to determine how different variables influenced physicochemical features, binding behaviour and immunoprecipitation of the target recombinant glutathione S-transferase tagged fusion protein (GST-CTer). Such variables included the cross-linking degree of MIN, and variables like pH, temperature or the use of Tween-20 for binding and IPP experiments. The cross-linker (CL) amount influenced the coil-to-globule transition of thermoresponsive MIN, making the lower critical solution temperature (LCST) decrease from 37.2 °C using 5% of CL, to 29.0 °C using 25%, also suggesting higher plasticity on the former. Temperature influence on size was corroborated by dynamic light scattering, observing size reductions from 250–450 nm (RT) to 70–100 nm (&gt; LCST) for MIN produced with 5–15% of CL. However, binding behaviour did not clearly  improve for more than 10% CL. Further experiments revealed that temperature and pH control were critical for efficient binding and release, selecting 40 °C and pH 5 as appropriate. Following binding experiments, the GST-CTer-MIN complex was successfully immunoprecipitated using MSB, achieving an IPP efficiency of 11.48% over the initial input protein concentration, which was calculated after SDS-PAGE separation and Western blot analysis. The methodology may be exploited for selective protein extraction and quantification from complex tissue homogenates.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06782-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent and colorimetric dual-mode recognition of Al3+ in tablets based on chrome azurol S and pH-switchable boron, nitrogen co-doped carbon dots","authors":"Liping Lin,&nbsp;Meng Fang,&nbsp;Yaojia Lin,&nbsp;Jinye Zhang,&nbsp;Wei Liu,&nbsp;Malin Cui","doi":"10.1007/s00604-024-06776-5","DOIUrl":"10.1007/s00604-024-06776-5","url":null,"abstract":"<div><p>The synthesis and characterization of pH-sensitive boron and nitrogen co-doped CDs (B, N-CDs) is reported. The fluorescence of B, N-CDs exhibited pH-responsive behavior within the range pH 3.0–6.0, attributed to their controlled aggregation and disaggregation. Interestingly, the fluorescence of B, N-CDs was quenched by the inner filter effect from chrome azurol S (CAS). With the subsequent addition of Al³⁺, the fluorescence of B, N-CDs was restored due to the formation of blue Al³⁺-CAS and B, N-CDs-Al³⁺ complexes. Consequently, a fluorescent and colorimetric dual-mode sensor for Al³⁺ detection was developed. The proposed sensor enabled Al³⁺ detection in the ranges and limits of detection of 3.75–22.5 μM and 2.67 μM for the fluorescent mode and 5–20 μM and 4.8 μM for the colorimetric mode, respectively. Furthermore, the efficacy of the sensor was validated through successful detection of Al³⁺ in tablet samples. The obtained B, N-CDs promised the application in acid condition and the proposed sensor is expected to be applied to in situ on-line detection of Al³⁺.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual metal–organic-frameworks mediated resonance energy transfer for ultrasensitive electrochemiluminescence immunosensing","authors":"Chun Wang,&nbsp;Gaoxing Cheng,&nbsp;Guixin Li,&nbsp;Jianbo Liu","doi":"10.1007/s00604-024-06796-1","DOIUrl":"10.1007/s00604-024-06796-1","url":null,"abstract":"<div><p>An electrochemiluminescence (ECL) biosensor for carcinoembryonic antigen (CEA) based on electrochemiluminescence resonance energy transfer (ECL-RET) was designed. Tris(2,2′-bipyridine)ruthenium(II) (Ru(bpy)₃²⁺) was used as an energy donor for ECL-RET, and an Au nanoparticle-modified MOF framework (AuCoFe MOF) was used as an energy receptor for ECL-RET. The ECL emission spectra of Ru(bpy)₃²⁺ were in the range 550 to 680 nm, and a zinc oxalate MOF encapsulating Ru(bpy)₃²⁺ (Ru@ Zn oxalate MOF) was prepared. The UV–vis absorption spectrum of AuCoFe MOF ranges from 280 to 700 nm and overlaps with emission spectra of Ru@Zn oxalate MOF, which is critical for RET. The AuCoFe MOF-Ab₂ bioconjugate, target CEA antigen, and the Ru@Zn oxalate MOF-Ab₁ bioconjugate together form a sandwich structure, resulting in quenching of the ECL signal of Ru@Zn oxalate MOF by AuCoFe MOF. Under the optimized experimental conditions, the ECL-RET sensor exhibited excellent analytical performance in CEA detection with a linear range of 1.0 × 10⁻¹³ to 1.0 × 10⁻⁸ mg mL⁻¹; the minimum limit of detection is 1.4 × 10⁻¹⁴ mg mL⁻¹ (<i>S</i>/<i>N</i> = 3); and its recoveries of spiked samples ranging from 99.1 to 100.7%. The developed sensor has excellent stability, reproducibility, and specificity and is suitable for the detection of CEA in human serum and has the potential to provide sensitive detection of other biomarkers of diseases.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of ultra-sensitive and selective hydrogen peroxide–based colorimetric sensor using highly exfoliated g-C3N4 nanosheets with peroxidase-like activity","authors":"Mohib Ullah,&nbsp;Roopashree R,&nbsp;Prakash Kanjariya,&nbsp;Mamata Chahar,&nbsp;Jasgurpreet Singh Chohan,&nbsp;Vineet Tirth,&nbsp;Hassan Alqahtani,&nbsp;Ali Algahtani,&nbsp;Tawfiq Al-Mughanam,&nbsp;Li Zhengxin","doi":"10.1007/s00604-024-06777-4","DOIUrl":"10.1007/s00604-024-06777-4","url":null,"abstract":"<div><p> A highly sensitive, portable, rapid, and accurate colorimetric sensing method is presented. It is based upon exfoliated g-C₃N₄ nanosheets (E–g-C₃N₄ NSs), having peroxidase nanozyme-like properties. The as-prepared catalyst (E–g-C₃N₄ NSs) tends to oxidize the colorless tetramethyl-benzidine (TMB) into oxidized-TMB in the presence of hydrogen peroxide (H₂O₂) generating a dark blue color and corresponding ultraviolet visible-spectral changes following a Michaelis–Menten kinetic. The prepared colorimetric sensor exhibited response within the range 0.001–0.450 μM having <i>R</i>² value of 0.999 and a detective limit (LOD) of 0.15 ± 0.04 nM. Furthermore, the sensor also displayed outstanding selectivity, ample stability (10 weeks), and excellent practicability in real sample applications. All these outstanding properties were highly attributed to the large surface area with exposed actives sites, high surface energy, and large conductive structure of E–g-C₃N₄ NSs. For comparison of the catalytic study, we have also explored the sensing mechanism of B-g-C₃N₄, using the same optimized experimental conditions. Resultantly, we concluded that the proposed sensor (E–g-C₃N₄ NSs) will gain considerable attention for on-site environmental and health monitoring in future endeavor.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cost-effective chemiresistive biosensor with MWCNT-ZnO nanofibers for early detection of tuberculosis (TB) lipoarabinomannan (LAM) antigen","authors":"Dinesh R. Rotake,&nbsp;Jitendra B. Zalke,&nbsp;Harsh V. Gechode,&nbsp;Sandhini M. Peshkar,&nbsp;Shiv Govind Singh","doi":"10.1007/s00604-024-06780-9","DOIUrl":"10.1007/s00604-024-06780-9","url":null,"abstract":"<div><p>The development of an affordable chemiresistive biosensor enhanced with a multi-walled carbon nanotube–zinc oxide (MWCNT-ZnO) nanofiber composite is presented. The sensor leverages the precise interaction between lipoarabinomannan (LAM) tuberculosis (TB) antigens and antibodies to achieve high sensitivity and specificity. The MWCNT-ZnO nanofibers have a larger surface area and better electrical conductivity, which makes it easier for TB antibodies to stick to them. The binding of LAM TB antigens to the fixed Monoclonal Antibody-MBS320597 induces significant resistance changes in the chemiresistive sensor, enabling accurate TB detection. Performance evaluation reveals a linear detection range from 1.0 to 100.0 pg/mL in the lower concentration range and up to 6.0 ng/mL in the higher concentration range, with a sensitivity of 79.750 mA pg mL⁻¹ cm⁻² and a lower limit of detection of 40.54 fg/mL. The sensor exhibits a response time of 102 s. Featuring rapid response time and high sensitivity, this biosensor is ideally suited for point-of-care (PoC) applications. The incorporation of MWCNT-ZnO nanofibers shows great potential for enhancing the development of sensitive and cost-effective TB diagnostic tools, which could play a crucial role in advancing global TB control and management efforts.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142540612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecularly imprinted electrochemiluminescence sensor based on luminol functionalized Co-MOF for rifampicin detection","authors":"Shuning Yang,&nbsp;Li Tian,&nbsp;Yongbo Feng,&nbsp;Yujia Song,&nbsp;Ruidan Li,&nbsp;Yanjia Guo,&nbsp;Huiling Li,&nbsp;Chao Li,&nbsp;Juan Lu","doi":"10.1007/s00604-024-06774-7","DOIUrl":"10.1007/s00604-024-06774-7","url":null,"abstract":"<div><p>A highly sensitive molecularly imprinted electrochemiluminescence (MIECL) sensor was developed for detecting rifampicin (RIF) based on luminol@Co-MOF. Co-MOF had a significant enhancement of ECL signaling in the luminol-O₂ system. Molecular imprinted polymers (MIPs) with the introduction of RIF provide new properties for the specific recognition of RIF. A noteworthy decrease in ECL intensity was observed with higher concentrations of RIF. Consequently, the ECL signal was controlled by RIF elution from and adsorption by the MIP, thus establishing a new method for RIF detection. Under optimal conditions, this sensor exhibited linear detection ranges of RIF between 1.0 × 10⁻¹¹ mol L⁻¹ and 1.0 × 10⁻⁶ mol L⁻¹, with a detection limit of 3.3 × 10⁻¹² mol L⁻¹ (<i>S</i>/<i>N</i> = 3). The recoveries ranged between 98.1 and 106.0% in fish samples. This method can be used as a sensitive and rapid method to detect RIF in real samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}