{"title":"A colorimetric fluorometric dual-mode aptasensor for aflatoxin B1 detection driven by Au@CeO2 core–shell nanozymes","authors":"Ziyue Chen, Lei Bai, Xinhua Xie, Xinhao Zhang, Shuaiqi Wen, Jieqiong Qin, Shichang Zhang, Bobo Zhang, Hongshuai Zhu","doi":"10.1007/s00604-025-07509-y","DOIUrl":null,"url":null,"abstract":"<div><p>A dual-mode aptasensor was engineered for aflatoxin B<sub>1</sub> (AFB<sub>1</sub>) detection by functional integration of peroxidase-mimetic Au@CeO<sub>2</sub> core–shell nanostructures with emissive carbon dots (CDs). The Au@CeO<sub>2</sub> nanocomposite, synthesized via spontaneous redox reaction, exhibited enhanced peroxidase-like activity due to abundant Ce<sup>3+</sup>/oxygen vacancies facilitating hydroxyl radical generation. The aptasensor utilizes a competitive binding mechanism, where AFB<sub>1</sub> competed with immobilized Au@CeO<sub>2</sub>-CDs-Apt1 probes for binding sites, resulting in inversely proportional colorimetric and fluorescent signals. Under optimized conditions, the biosensor achieved a broad linear detection range (0.001–50 ng/mL) with an ultralow limit of detection (LOD) of 0.0005 ng/mL. The sensor demonstrated excellent selectivity, stability, and reproducibility, and was successfully applied to detect AFB<sub>1</sub> in spiked peanut samples, with recoveries ranging from 98.7 to 116.7%. This work not only advances the design of nanozyme-based biosensors but also provides a rapid, accurate, and field-deployable strategy for monitoring mycotoxins in complex food matrices.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07509-y","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
A dual-mode aptasensor was engineered for aflatoxin B1 (AFB1) detection by functional integration of peroxidase-mimetic Au@CeO2 core–shell nanostructures with emissive carbon dots (CDs). The Au@CeO2 nanocomposite, synthesized via spontaneous redox reaction, exhibited enhanced peroxidase-like activity due to abundant Ce3+/oxygen vacancies facilitating hydroxyl radical generation. The aptasensor utilizes a competitive binding mechanism, where AFB1 competed with immobilized Au@CeO2-CDs-Apt1 probes for binding sites, resulting in inversely proportional colorimetric and fluorescent signals. Under optimized conditions, the biosensor achieved a broad linear detection range (0.001–50 ng/mL) with an ultralow limit of detection (LOD) of 0.0005 ng/mL. The sensor demonstrated excellent selectivity, stability, and reproducibility, and was successfully applied to detect AFB1 in spiked peanut samples, with recoveries ranging from 98.7 to 116.7%. This work not only advances the design of nanozyme-based biosensors but also provides a rapid, accurate, and field-deployable strategy for monitoring mycotoxins in complex food matrices.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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