An electrochemical immunosensor based on HFABP for the early detection of drug-induced cardiac toxicity from traditional Chinese medicines

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-09-03 DOI:10.1007/s00604-025-07420-6

Bolu Sun, Jiali Kang, Zixia Wang, Yuqiong Wu, Haiying He, Xinlan Wang, Xuanxiu Da, Miao Zhou, Ying Lv, Chenyu Qin, Xiangdong Wang, Lin Yang, Huajie Li, Hai Wang

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引用次数: 0

Abstract

An electrochemical immunosensor was successfully developed for evaluating traditional Chinese medicine (TCM)-induced cardiac toxicity by immobilizing heart-type fatty acid-binding protein (HFABP)-specific antibodies on an electrode surface modified with chitosan (CS)-functionalized metal–organic framework ZIF-8 and gold nanoparticles (AuNPs). Leveraging the excellent biocompatibility of AuNPs, the excellent stability and high surface area of ZIF-8, along with the strong specific recognition capability of HFABP antibodies, the sensor demonstrated superior performance. Under optimized experimental conditions, it achieved an ultra-low detection limit of 7.09 × 10⁻² pg mL⁻¹ for HFABP, maintained excellent linearity in the range 1.57 × 10⁻² ~ 1.60 × 10¹ ng mL⁻¹, and outperformed conventional ELISA methods in sensitivity when applied to TCM-induced cardiac toxicity models. The sensor also demonstrated excellent specificity, reproducibility, and stability. This research provides a sensitive and rapid new method for early warning of TCM-induced cardiac toxicity and contributes technical support and scientific reference for improving diagnostic accuracy in herbal medicine safety assessment.

Graphical Abstract

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基于HFABP的电化学免疫传感器用于中药药物性心脏毒性的早期检测

通过将心脏型脂肪酸结合蛋白（HFABP）特异性抗体固定在壳聚糖（CS）功能化金属-有机骨架ZIF-8和金纳米颗粒（AuNPs）修饰的电极表面，成功研制了一种用于评价中药诱导的心脏毒性的电化学免疫传感器。利用aunp优异的生物相容性、ZIF-8优异的稳定性和高表面积，以及对HFABP抗体较强的特异性识别能力，该传感器表现出优越的性能。在优化的实验条件下，HFABP的超低检出限为7.09 × 10−2 pg mL−1，在1.57 × 10−2 ~ 1.60 × 101 ng mL−1范围内保持良好的线性关系，用于中药致心脏毒性模型的灵敏度优于传统ELISA方法。该传感器还表现出良好的特异性、重复性和稳定性。本研究为中药心脏毒性早期预警提供了一种灵敏、快速的新方法，为提高中药安全性评价的诊断准确性提供了技术支持和科学参考。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.