Microchimica Acta最新文献

{"title":"A SERS sensing platform based on the redox reaction of 3,3',5,5'-tetramethylbenzidine for the rapid detection of dopamine","authors":"Jinkun Yan,&nbsp;Qijia Zhang,&nbsp;Xiaodi Ma,&nbsp;Guangda Xu,&nbsp;Ce Gao,&nbsp;Lefa Zhao","doi":"10.1007/s00604-025-07519-w","DOIUrl":"10.1007/s00604-025-07519-w","url":null,"abstract":"<div><p> A surface enhanced Raman scattering (SERS)-based sensing platform is devised integrating a TMB redox system for rapid dopamine detection. Gold nanobipyramids (Au NBPs), synthesized via the heat-mediated seed-mediated growth method, possess dual functionality of peroxidase-like activity and SERS activity. This enables them to catalyze the oxidation of TMB and simultaneously amplify the Raman signal of the oxidized TMB product (oxTMB). Whereas, the phenolic hydroxyl groups within the dopamine structure reduce oxTMB, leading to a decrease in its Raman signal. By utilizing the change in signal intensity during the TMB redox process, a quantitative detection model for DA was successfully established. Experimental results demonstrate that this sensing platform exhibits a good linear response (R² = 0.997) within the concentration range 1 nM to 10 μM, with a detection limit of 1 nM. It also displays significant anti-interference capabilities against various common interferents such as Na⁺, K⁺, Cl⁻ and glucose. In the detection of spiked athlete serum samples, recoveries ranged from 81.24% to 109.98%. This rapid DA detection strategy provides a novel technical approach for the rapid assessment of physiological functional status and demonstrates significant application prospects.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145011820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-mode nanozyme-hydrogel platform for on-site multi-target detection of biomarkers and hazardous substances","authors":"Junjie Zhao,&nbsp;Jiawei Hong,&nbsp;Haonan Li,&nbsp;Haonan Zhang,&nbsp;Longshan Zhao","doi":"10.1007/s00604-025-07516-z","DOIUrl":"10.1007/s00604-025-07516-z","url":null,"abstract":"<div><p> A novel dual-mode sensing system integrating a magnetic core–shell CuFe₂O₄/Cu/MnO₂ nanozyme with a stimuli-responsive agarose-deep eutectic solvent hydrogel (DES-Aga) is reported. The nanozyme exhibits exceptional oxidase-like activity, characterized by a low Michaelis constant (K_m = 0.14 mM) and high catalytic efficiency (V_max = 1.89 × 10⁻⁶ M·s⁻¹), enabling rapid oxidation of TMB to generate a colorimetric signal. Coupled with the DES-Aga hydrogel, the platform achieves dual-mode detection: laboratory-grade UV–vis quantification (detection limits: 0.01 μM HQ, 0.05 μM GSH, 0.02 μM NO₂⁻) and smartphone-assisted on-site analysis. The hydrogel leverages redox/diazotization interactions to produce distinct color transitions (blue → colorless for HQ/GSH; blue → yellow for NO₂⁻), validated in real-world matrices (cosmetics, food, serum) with recoveries of 87–115% and RSD &lt; 8.6%. Key innovations include the nanozyme’s magnetic recyclability (&gt; 80% activity after 7 cycles), the hydrogel’s stability (&gt; 90% retention after 7 days), and a ratiometric strategy for NO₂⁻ detection. This work bridges the gap between laboratory precision and field-deployable diagnostics, offering a versatile tool for monitoring carcinogens in consumer products, food contaminants, and oxidative stress biomarkers, with direct implications for public health and safety.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145011612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A highly active self-assembled nanozyme based on cascade system for dual-mode detection of sarcosine","authors":"Yanke Zhai,&nbsp;Dan Wang,&nbsp;Qianqing Lin,&nbsp;Ruixin Yang,&nbsp;Guo Chen,&nbsp;Xiaotong Li,&nbsp;Haonan Jin,&nbsp;Hualu Zhang,&nbsp;Yibing Ji,&nbsp;Ruijun Li","doi":"10.1007/s00604-025-07501-6","DOIUrl":"10.1007/s00604-025-07501-6","url":null,"abstract":"<div><p>A nanozyme-mediated cascade reaction system for fluorometric and colorimetric dual-mode detection of sarcosine (SA) was developed. The nanozymes (Zn-Glu@Hemin) were synthesized via a rapid self-assembly within 10 min at room temperature. Importantly, the Zn-Glu@Hemin exhibited strong peroxidase (POD)-mimicking activity, catalyzing the generation of hydroxyl radical (·OH) and superoxide anion (O₂^·−) from hydrogen peroxide (H₂O₂), enhancing the fluorescence reaction of o-phenylenediamine (OPD) and the colorimetric reaction of 3,3′,5,5′-tetramethylbenzidine (TMB). Thus, a dual-mode sensing platform is created by combining SA and sarcosine oxidase (SOD) into the enzyme cascade system (SA-SOD-POD). The developed sensors exhibited superior performance, with high sensitivity and selectivity for SA detection. The detection limits were 0.14 μM (fluorescence) and 0.26 μM (colorimetric), with detection ranges of 1–120 μM and 1–70 μM, respectively. The system was incorporated into both a smartphone-assisted dual-signal sensing platform and a portable field detection kit, demonstrating practical applicability. Besides, the practicability of the established approach was confirmed in human serum samples with reliable recoveries. This work shows advantages in the synthesis method and detection sensitivity of nanozymes, which can accurately detect disease biomarkers and show high  potential in real-time diagnosis.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145011692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Titanium dioxide nanoparticles as a promising tool for efficient separation of trace DNA via phosphate-mediated desorption","authors":"Song Li,&nbsp;Jing Tang,&nbsp;Yalong Bai,&nbsp;Chunmin Pu,&nbsp;Xiaoyan Liao,&nbsp;Lili Chen","doi":"10.1007/s00604-025-07511-4","DOIUrl":"10.1007/s00604-025-07511-4","url":null,"abstract":"<p>We systematically evaluated the DNA adsorption and desorption efficiencies of several nanoparticles. Among them, titanium dioxide (TiO₂) nanoparticles (NPs), aluminum oxide (Al₂O₃) NPs, and zinc oxide (ZnO) NPs exhibited strong DNA-binding capacities under mild conditions. However, phosphate-mediated DNA displacement efficiencies varied considerably, with only TiO₂ NPs showing consistently superior performance. Further investigation into the DNA adsorption and desorption mechanisms of TiO₂ NPs led to the following key results: (1) TiO₂ NPs achieved over 98% DNA adsorption at room temperature, but efficient desorption required elevated temperatures; (2) phosphate-induced DNA displacement depended on the full exposure of phosphate groups, and short DNA fragments were insufficient to effectively compete with adsorbed DNA; (3) the adsorption mechanism of TiO₂ NPs involved multiple interactions, such as coordination and hydrogen bonding. The combination of strong coordination and weak ionic forces likely contributed to the high efficiency of phosphate-mediated desorption. Under optimized conditions, TiO₂ NPs demonstrated excellent separation efficiency for structurally complex DNA, with recovery rates of 56.92% for genomic DNA and 66.31% for plasmid DNA, notably higher than those of amino-modified silica-coated magnetic nanoparticles (ASMNPs; 38.66% and 33.59%). These results highlight the potential of TiO₂ NPs as a powerful tool for trace DNA isolation under mild, biocompatible conditions, with promising applications in nucleic acid separation and molecular diagnostics.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145011694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanocomposite enhanced molecularly imprinted polymer for electrochemical detection of naringenin in plant-based samples","authors":"Marija Milićević,&nbsp;Ahmed Isa,&nbsp;Seyda Yayla,&nbsp;Danica Bajuk-Bogdanović,&nbsp;Damjan Vengust,&nbsp;Matjaž Spreitzer,&nbsp;Ahmet Cetinkaya,&nbsp;M. Mesud Hurkul,&nbsp;Sonja Jovanović,&nbsp;Sibel A. Ozkan","doi":"10.1007/s00604-025-07489-z","DOIUrl":"10.1007/s00604-025-07489-z","url":null,"abstract":"<div><p> A novel molecularly imprinted polymer (MIP)-based electrochemical sensor has been developed for the selective detection of naringenin (NAR) in various real-world samples, including plant extracts, wine, and herbal supplements. To enhance the active surface area and porosity of the glassy carbon electrode (GCE), a 2D/0D nanocomposite composed of graphene oxide (GO) and cobalt ferrite (CFO) nanoparticles, CFO_GO, was incorporated into the sensor design. 4-aminobenzoic acid (4-ABA) was selected as the functional monomer to prepare the MIPs. The polymerization process was performed using ethylene glycol dimethacrylate (EGDMA) as the crosslinking agent, 2-hydroxyethyl methacrylate (HEMA) as the basic monomer, and 2-methylpropiophenone as the initiator. The developed MIP-based sensor was designed for the electrochemical detection of NAR in real samples such as <i>Solanum lycopersicum</i> L. (tomato) fruit, <i>Citrus</i> × <i>limon</i> (L.) Osbeck (lemon), oak (Quercus) bark, red wine, and herbal supplements demonstrate their potential for practical applications in analyzing food and herbal products. Morphological and electrochemical characterizations of the designed NAR/CFO_GO/4-ABA@MIP-GCE sensor were performed using scanning electron microscopy (SEM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). The linear range for the determination of NAR using the indirect method (5.0 mM [Fe(CN)₆]^−3/−4) was found to be 1.0 × 10^–13 M-1.0 × 10^–12 M, and the limit of detection (LOD) and limit of quantification (LOQ) for standard solutions were 2.84 × 10^–14 and 9.47 × 10^–14 M, respectively. As a result of the study, the developed MIP-based electrochemical sensor was suitable for detecting NAR with high specificity, selectivity, and sensitivity. Additionally, recovery studies were performed to determine the practical applicability of the sensor, and the results were satisfactory. The developed sensor platform can be considered a reliable and sensitive analytical tool for determining NAR.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145011693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hollow polydopamine for the enhanced ultrasensitive dual-modal immunochromatographic detection of Salmonella typhimurium in dairy products via machine learning","authors":"Fei Liu,&nbsp;Chenjie Nie,&nbsp;Ziqi Wang,&nbsp;Kunyue Han,&nbsp;Wenrui Zhang,&nbsp;Yanwei Ji,&nbsp;Guangjun Huang,&nbsp;Wentao Zhang,&nbsp;Jianlong Wang","doi":"10.1007/s00604-025-07430-4","DOIUrl":"10.1007/s00604-025-07430-4","url":null,"abstract":"<div><p><i>Salmonella typhimurium</i> (<i>S. typhimurium</i>) A dual-mode colorimetric/photothermal immunochromatographic strip (ICS) employing hollow polydopamine nanoparticles (h-PDA) is reported for the ultrasensitive detection of <i>Salmonella typhimurium (S. typhimurium</i>). The h-PDA was synthesized via a coordination chemistry-mediated etching of zeolite imidazolate frameworks-8, yielding a hollow nanostructure that enhances photothermal conversion efficiency (52.36%) through the increased surface area and reduced thermal loss. The abundant catechol hydroxyl groups on h-PDA significantly improve antibody coupling efficiency. This ICS achieves detection limits of 603 CFU mL⁻¹ (colorimetric) and 427 CFU mL⁻¹ (photothermal)—a 16-fold improvement over conventional AuNP-based strips. The integration of a single-layer artificial neural network (ANN) enables 100% classification accuracy for <i>S</i>. <i>typhimurium</i>. The feasibility of the developed method is demonstrated by the recoveries of 99.71–111.72% (RSD &lt; 18.73%) in spiked milk samples. This work advances photothermal nanomaterial design for ICS platforms and provides a robust tool for the rapid surveillance of foodborne pathogens.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145011819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous determination and degradation of aflatoxin B1 using a covalent-organic framework initiated hydrogel and aptamer-based colorimetric sensing platform","authors":"Man Gong,&nbsp;Lingling Zhou,&nbsp;Liurunzi Xv,&nbsp;Meng Wang,&nbsp;Qingli Yang,&nbsp;Xiudan Hou","doi":"10.1007/s00604-025-07529-8","DOIUrl":"10.1007/s00604-025-07529-8","url":null,"abstract":"<div><p>As the most dangerous mycotoxin, aflatoxin B1 (AFB1) has caused some food safety issues to be concerned. In this study, a simultaneous detection and degradation method towards AFB1 was established. Covalent-organic frameworks (COFs) were firstly synthesized and directly in situ deposited on the stainless-steel mesh, which would trigger the free-radical polymerization of acrylamide to form a hydrogel coating. The synthesized Pt@Au nanoparticles as a colorimetric signal probe exhibited the high peroxidase catalytic activity (<i>K</i>_m = 0.14 mM, <i>V</i>_max = 4.57 × 10⁻⁸ mol (L s)⁻¹, <i>K</i>_cat = 1.40 × 10⁶ s⁻¹). Coupled with a smartphone application, the prepared “sandwich-structure” colorimetric sensor enables detection of AFB1 with a desirable linear range (0.4–200 μg L⁻¹) and a low detection limit (0.085 μg L⁻¹). The porous channel of the COF-based hydrogel prevents the interference of other macromolecules due to the size effect, resulting in a desirable matrix effect of 92.09–104.71%. The recoveries spiked with different concentrations of AFB1 were 93.75–132.10%. The degradation efficiency of AFB1 could reach 91.74% within 180 min. Through the investigation using ESI–MS, four degraded intermediate products were identified to form the possible degraded pathway. Overall, the fabrication of COFs-based sensing platform provided a new approach to detect and degrade mycotoxins.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145011818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-free immunoassay based on chemiluminescence-functionalized magnetic mesoporous nanoparticles for rapid detection of neuron-specific enolase","authors":"Wenxia Chen,&nbsp;Shuangyi Hu,&nbsp;Bingqian Zhou,&nbsp;Yueran Ren,&nbsp;Yangyang Xue,&nbsp;Rui Yang","doi":"10.1007/s00604-025-07475-5","DOIUrl":"10.1007/s00604-025-07475-5","url":null,"abstract":"<div><p>Lung cancer, as one of the cancers with the highest morbidity and mortality rates in the world, requires accurate detection of its vital serum marker, neuron-specific enolase (NSE), which is a key challenge for early detection of lung cancer. However, traditional chemiluminescence immunoassay (CLIA) methods rely on labeled antibodies (Abs) and suffer from complex operations and high costs. In this work, a label-free CLIA based on CL-functionalized mesoporous magnetic nanoparticles (CuFe₂O₄@mSiO₂-Cys-Luminol-Au NPs) is developed for the rapid and sensitive detection of NSE. The material combines the advantages of magnetic separation, mesoporous loading, catalytic enhancement, and biorecognition. CuFe₂O₄ NPs offer efficient magnetic separation, while the mesoporous silica (mSiO₂) layer is effectively loaded with luminol to amplify CL signals. L-cysteine (L-Cys) ensures the uniform distribution of luminol and gold nanoparticles (Luminol-Au NPs), with these Au NPs immobilizing Abs through Au–S and Au–N bonds, achieving high-density biocoupling. The constructed label-free CLIA enables highly sensitive detection of NSE without Abs labeling. The results show that the NSE concentration is linearly correlated with the CL intensity in the range 1.0 × 10⁻¹³ ~ 1.0 × 10⁻⁸ g/mL, and the detection limit is as low as 2.3 × 10⁻¹⁴ g/mL. The proposed method demonstrates excellent selectivity, stability, and anti-interference capability for the detection of NSE in human serum samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly sensitive detection and mechanism study of tetracycline in water by CuFeO2/NiCo-LDH heterojunction electrochemical sensor","authors":"Aijin Xiao,&nbsp;Meng Li,&nbsp;Yiluo Hu,&nbsp;Wei Zhang,&nbsp;Huiping Bai,&nbsp;Xuejun Pan,&nbsp;Fengzhi Jiang","doi":"10.1007/s00604-025-07508-z","DOIUrl":"10.1007/s00604-025-07508-z","url":null,"abstract":"<div><p>A CuFeO₂/NiCo-LDH heterojunction electrochemical sensor (LDH: layered double hydroxide) was developed for the sensitive detection of tetracycline (TC). The sensor was constructed by integrating ZIF-67-derived nanocage NiCo-LDH on nickel foam with CuFeO₂, forming a p-n heterojunction that enhanced electron transfer and TC adsorption. The sensor exhibited bilinear detection ranges (0.00594 ~ 1 and 1 ~ 25 μM) and a low detection limit of 1.98 nM. Recoveries in real water samples ranged from 92.0 to 109.0%, with relative standard deviations not exceeding 4.5%. In situ Raman spectroscopy and density functional theory calculations confirmed the involvement of multiple redox pairs (Ni³⁺/Ni²⁺, Co³⁺/Co²⁺, Fe³⁺/Fe²⁺ and Cu²⁺/Cu⁺) and strong interfacial adsorption energy. This sensor presents a practical and efficient approach for onsite TC monitoring.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MOF-templated hollow Pd/CdS@Co3S4 nanocages with synergistic Z-scheme/Schottky effects for photoelectrochemical biosensing of chlorpyrifos featuring exceptional dynamic range","authors":"Shipeng Huang,&nbsp;Haicai Huang,&nbsp;Jingqiu Liu,&nbsp;Haoyu Duan,&nbsp;Xi Chen,&nbsp;Houyang Chen","doi":"10.1007/s00604-025-07469-3","DOIUrl":"10.1007/s00604-025-07469-3","url":null,"abstract":"<div><p> A novel ternary synergistic photoelectrochemical (PEC) probe is presented utilizing metal–organic framework (MOF)-templated Pd/CdS@Co₃S₄ nanocages for sensing chlorpyrifos (CPF) using chronoamperometry under an applied bias of − 65 mV with 465-nm LED illumination. Derived from ZIF-67 via in situ sulfidation, the hollow nanocage architecture integrated CdS nanoparticles with Co₃S₄ to form a direct Z-scheme heterojunction, while decorating Pd quantum dots (QDs) created a Schottky barrier, implementing a crucial dual charge-transfer enhancement strategy. Density functional theory (DFT) simulations confirmed a 0.36-eV Fermi level difference at heterojunction interface, verifying a forced built-in electric field. The optimized Pd/CdS@Co₃S₄ nanocomposite exhibited a remarkable 4.63-fold photocurrent amplification over its pristine Co₃S₄, establishing a high-intensity signal baseline essential for accommodating wide-range concentration-dependent signal attenuation. Acetylcholinesterase (AChE)-immobilized biosensor quantified CPF via inhibition-triggered competitive electron consumption to attenuate photocurrent. The sensor demonstrated exceptional performance for CPF detection, most notably featuring a linear dynamic range spanning four orders of magnitude (0.1 ~ 2000 ng·mL⁻¹). Furthermore, it achieved a low detection limit (0.05 ng·mL⁻¹, <i>S</i>/<i>N</i> = 3), outstanding specificity against interfering species, excellent long-term stability, and reliable accuracy in complex real water samples (96.5 ~ 104.5%). This study proposes dual charge-transfer enhancement strategy and hollow architecture, addressing the broad-concentration-range in environmental pesticide detection with good sensitivity and adaptability to real-world matrices.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144998569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}