Microchimica Acta最新文献

{"title":"Recent advances in MOF–phosphate interaction-based bioanalysis: from enrichment to sensing","authors":"Zhao-Di Ding,&nbsp;Qi-Yan Lv,&nbsp;Xiaojie Song,&nbsp;Junyang Chen,&nbsp;Bing Yu,&nbsp;Hang Fan,&nbsp;Hui-Fang Cui","doi":"10.1007/s00604-026-08371-2","DOIUrl":"10.1007/s00604-026-08371-2","url":null,"abstract":"<div><p>Molecular recognition serves as the foundation of bioanalytical strategies. Typical recognition mechanisms include antigen−antibody binding, biotin−streptavidin interaction, aptamer−target pairing, lectin−saccharide recognition, and the chelation of metal ions with phosphate groups, among others. Among these, the chelation of metal−organic frameworks (MOFs) with phosphate groups has emerged as a particularly concise and efficient molecular recognition strategy. In recent years, MOF−phosphate interactions have been extensively exploited in diverse bioanalytical platforms, highlighting their remarkable potential for practical applications. In this review, we summarize MOF−phosphate-based bioanalytical strategies, with a focus on their use in the enrichment of phospholipids and phosphopeptides, kinase activity assays, and the detection of various phosphate-containing analytes. Furthermore, we discuss the development of multifunctional MOF composites enabled by this interaction and conclude with perspectives on the opportunities and challenges that lie ahead.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interfacial tripedal DNA walking coupled with oxygen-depletion/ECL-RET dual quenching for femtomolar detection of Tobacco Mosaic Virus RNA in plant extracts","authors":"Jinghan Yu,&nbsp;Liang Guo,&nbsp;Zhixiang Liu,&nbsp;Huaixia Yang,&nbsp;Weisheng Feng","doi":"10.1007/s00604-026-08366-z","DOIUrl":"10.1007/s00604-026-08366-z","url":null,"abstract":"<div><p>Sensitive quantification of Tobacco Mosaic Virus RNA (TRNA) is imperative for preventing large-scale agricultural losses. In this study, we engineered a sophisticated quenching-based electrochemiluminescence (ECL) biosensor by integrating a high-performance CdTe@ZnS nanoscaffold with a target-triggered tripedal DNA walker. To ensure a robust and stable initial luminophore density, multi-walled carbon nanotubes (MWCNTs) were utilized to anchor CdTe@ZnS core–shell quantum dots, where the ZnS shell effectively passivates surface defects and prevents cadmium leakage. Upon recognition of TRNA, a catalytic hairpin assembly (CHA) and toehold-mediated strand displacement (TMSD) cascade are initiated, driving the tripedal DNA walker to autonomously navigate the electrode surface. This movement recruits a multitude of Cu₂O nanoparticles (quenchers) to the sensing interface, which efficiently suppresses the ECL emission through the consumption of the co-reactant (O₂) and dual-pathway quenching. Benefiting from the synergistic amplification of the MWCNT carrier and the high walking efficiency of the multivalent DNA machine, the biosensor achieved a broad linear range from 80 fM to 100 nM, with a remarkable detection limit of 9.18 fM. The successful discrimination of TRNA from mismatched sequences and its performance in complex matrices underscore the potential of this platform for early-stage phytopathogen diagnostics.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148918033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric and photothermal dual-mode sensing platform for catecholamines detection based on an irregular octahedral CoFe bimetallic organic framework nanozyme","authors":"Runhong Liu,&nbsp;Haitao Liu,&nbsp;Junyi He,&nbsp;Rongrong Cao,&nbsp;Xuewen Qin,&nbsp;Jiaming Cheng,&nbsp;Ling Wu,&nbsp;Dan Li,&nbsp;Donghong Yu,&nbsp;Zhong Cao","doi":"10.1007/s00604-026-08390-z","DOIUrl":"10.1007/s00604-026-08390-z","url":null,"abstract":"<div><p>Accurate and efficient detection of catecholamines (CAs) is of great importance for the diagnosis and treatment of neuroendocrine-related diseases. However, developing convenient sensing platforms that combine high sensitivity, high selectivity, and multi-mode validation capabilities still faces challenges. To address this issue, an irregular octahedral cobalt-iron bimetallic organic framework (<i>io</i>-CoFe-MIL) nanozyme with excellent peroxidase-like activity was synthesized via a solvothermal method. The nanozyme can efficiently catalyze the oxidation of substrate 3,3’,5,5’-tetramethylbenzidine (TMB), generating blue oxidized TMB (TMBox). The inhibitory effect of CAs on such TMB catalytic oxidation process leads to changes in both solution color and system temperature. Thus, a colorimetric/photothermal dual-mode sensing platform was constructed for catecholamines detection, as epinephrine (EP), dopamine (DA), and norepinephrine (NE). The colorimetric sensor exhibited sensitive responses toward various CAs with limit of detection (LOD) of 0.03 µM for EP, 0.055 µM for DA, and 0.08 µM for NE. While in the photothermal mode, the LODs for EP, DA, and NE were 0.027 µM, 0.74 µM and 1.2 µM, respectively. The presented dual-mode methods can be well used for sensitive detection of EP, DA, and NE in actual porcine serum samples, that are in consistent with those obtained from the instrument of HPLC, demonstrating the reliability and practical applicability of the method. This work provides a promising strategy for highly sensitive and multi-mode detections of CAs based on the synergistic integration of colorimetric and photothermal dual-signal responses.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-mode colorimetric and RGB detection of sulfide based on a magnetic Fe3O4/CoFe2O4/Ce-BDC hybrid nanozyme","authors":"Qingyuan Weng,&nbsp;Xinyi Wen,&nbsp;Tianyuan Wei,&nbsp;Qing Zhang,&nbsp;Wanying Zhu,&nbsp;Junli Hong","doi":"10.1007/s00604-026-08375-y","DOIUrl":"10.1007/s00604-026-08375-y","url":null,"abstract":"<div><p>A dual-mode sensing strategy is presented by integrating a magnetic Fe₃O₄/CoFe₂O₄/Ce-BDC (denoted as FCF/Ce-BDC) nanozyme with TMB/H₂O₂ chromogenic chemistry to construct peroxidase-mimetic hybrids. The FCF/Ce-BDC catalyst efficiently oxidizes 3,3’,5,5’-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide (H₂O₂), generating a blue product (oxTMB, <i>λmax</i> = 651 nm). The subsequent reaction of sulfide under acidic conditions scavenges reactive oxygen species (ROS), predominantly hydroxyl radicals (•OH), and reduces oxidized 3,3’,5,5’-tetramethylbenzidine (oxTMB), leading to a pronounced decrease in absorbance at 651 nm and a visually discernible color transition from blue to colorless. This dual-signal response enables quantitative determination of sulfide via both conventional spectrophotometric absorbance measurement and smartphone-based RGB colorimetry, achieving detection limits of 0.29 μM (absorbance mode) and 7.43 μM (RGB mode), respectively. We fabricated three portable, field-deployable sensors for on-site sulfide detection: a hydrogel film, a cotton fabric strip and a cotton swab. The hydrogel sensor leverages ion-mediated crosslinking to modulate reaction kinetics and ensure reproducible color development, whereas the cotton-based substrates exploit capillary-driven fluid transport to enrich analyte concentration, thereby enhancing sensitivity and mitigating matrix interference. This study not only advances the rational design of multifunctional nanozymes but also delivers a validated, application ready framework for their safe and selective use in environmental water matrices and human whole blood, enabling real time, cost effective, and operationally simple tracking of target pollutants without compromising analytical reliability.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of Au/RuO2 multifunctional nanocomposite and its application in chemodynamic-photothermal synergistic antibacterial","authors":"Yingying Zhong,&nbsp;Junsong Yang,&nbsp;Jia Liu,&nbsp;Xiaorui Lu,&nbsp;Chengxin Lin,&nbsp;Sujian Cao,&nbsp;Minglei Lu,&nbsp;Hongwu Wang","doi":"10.1007/s00604-026-08374-z","DOIUrl":"10.1007/s00604-026-08374-z","url":null,"abstract":"<div><p>Food safety and public health are severely compromised by foodborne pathogens such as <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and <i>Escherichia coli</i> (<i>E. coli</i>). Nevertheless, there are still significant challenges in developing effective and stable antibacterial approaches. Herein, an Au/RuO₂ nanocomposite with a clear and well-defined core-shell structure was synthesized via an one-step hydrothermal by tuning the Au and Ru precursor contents. Benefiting from its exceptional peroxidase-like (POD-like) activity (<i>K</i>_<i>m</i>: 0.15 mM for TMB, 5.68 mM for H₂O₂) and high photothermal conversion efficiency (85.39%), the nanocomposite was used in antibacterial purposes. The results demonstrated that at a low concentration of 2.88 µg/mL, complete killing of <i>S. aureus</i> and <i>E. coli</i> was achieved via the generation of reactive oxygen species (ROS) (hydroxyl radicals (·OH) and singlet oxygen (¹O₂)), localized temperature elevation, biofilm suppression and biofilm eradication. The antibacterial performance was markedly superior to that of single-agent treatments (H₂O₂ or 808 nm laser alone, requiring ~ 11.54 µg/mL), underscoring its broad-spectrum and highly efficient chemodynamic-photothermal (CDT-PTT) synergistic antibacterial potential. Moreover, the Au/RuO₂ nanocomposite also exhibited excellent stability (could maintain its original activity after 90 days of storage at 4 °C and 20 days at room temperature), good reusability (could retain over 90% of its original activity after five cycles) and high biocompatibility (no toxicity to multiple cells at 100 µg/mL, and with a hemolysis rate below 2%), which made it a potential material for efficient and safe antibacterial applications. In summary, the Au/RuO₂ nanocomposite exhibited excellent and stable POD-like activity, photothermal properties, synergistic antibacterial effects and biocompatibility, which not only enrich the family of multifunctional nanomaterials but also offer a promising candidate for antibiotic-free antibacterial strategies.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone-based visual quantitation of glyphosate in liquid media using effervescent colorimetric tablets encompassing Ru-doped MOF with augmented enzyme-like activity","authors":"Yong Pan,&nbsp;Xiaofeng Hou,&nbsp;Chengyu Li,&nbsp;Xuedong Wang,&nbsp;Tingting Liu","doi":"10.1007/s00604-026-08395-8","DOIUrl":"10.1007/s00604-026-08395-8","url":null,"abstract":"<div><p>Although glyphosate is widely applied as a non-selective herbicide in agricultural production, its rapid and on-site monitoring remains challenging. Building upon magnetic effervescent tablets (METs) previously developed by our group, we designed novel METs-based colorimetric tablets (MCTs) using Ru-doped Fe-MOF (Ru₄₀/Fe-MOF) with significantly enhanced peroxidase-mimetic activity. MCTs were integrated with smartphone-based digital-image chromogenesis (SDC) for visual quantitation of glyhosate in surface waters and liquid drinks. Ru-doping not only increased the specific surface area and regulated MOFs’ morphology, but also prominently improved the underlying catalytic activity. Upon the addition of H₂O₂, Ru₄₀/Fe-MOF catalyzed the chromogenic substrate ABTS to form a green oxidized product oxABTS, yielding a bright green solution. However, glyphosate could inhibit the aforementioned catalytic activity through coordination and/or H-bonding interactions with the surface groups of Ru₄₀/Fe-MOF (-COOH, -NH₂, Fe-OH, and Ru sites). MCTs enabled rapid auto-dispersion and chromogenic development upon contact with water, thereby eliminating the need for external stirring, shaking or heating. Under optimized conditions, the MCTs-SDC platform exhibited an excellent linearity for glyphosate with a detection limit of 0.006 µg/mL by UV-vis measurement and 0.03 µg/mL by grayscale analysis via a self-developed “Thing Identify” APP. In the lake water and liquid drink samples, the fortified recoveries (94.5-112.2%) by the MCTs-SDC were comparable to those by conventional water-bath-assisted colorimetry and HPLC-DAD analysis. Overall, this work provides a user-friendly, rapid, and on-site smartphone-based visual quantitation scheme for glyphosate in complex liquid media. </p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148910578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a self-signaling electrochemical sensor utilizing CoNiFe-PBA for detection of Bisphenol A in food samples","authors":"Zitong Yang,&nbsp;Jingyu Deng,&nbsp;Yili Zhang,&nbsp;Wenkai Qi,&nbsp;Yuxia Zhang","doi":"10.1007/s00604-026-08367-y","DOIUrl":"10.1007/s00604-026-08367-y","url":null,"abstract":"<div><p>A self-signaling electrochemical sensor based on molecular imprinting technology for the detection of Bisphenol A (BPA) in food samples. The sensor was constructed through sequential modification of the electrode with reduced graphene oxide (RGO) and CoNiFe Prussian blue analogue (CoNiFe-PBA), followed by electropolymerization of an MIP film using BPA as template and o-phenylenediamine as monomer. CoNiFe-PBA provides an intrinsic signal at ~ 0.47 V without external probes, originating from synergistic redox interactions among Co, Ni, and Fe. BPA rebinding reduces the signal via steric hindrance, enabling sensitive detection in food samples. Under optimal conditions, the sensor demonstrated a wide linear detection range (0.5–500 nM) and a low detection limit (0.19 nM). Owing to its high selectivity, sensitivity, and accuracy, the sensor offers a reliable approach for precise quantification of trace BPA in complex food matrices.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148904999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasmall Ag₂Se@Mn quantum dots enable dual-modal imaging-guided early detection and antioxidant therapy of acute kidney injury","authors":"Yun Qu,&nbsp;Yiping Gu,&nbsp;Qinzhi Zeng,&nbsp;Xianbin Ma,&nbsp;Chao Li,&nbsp;Yao Lei,&nbsp;Hai-Yan Xie,&nbsp;Ran Cui","doi":"10.1007/s00604-026-08389-6","DOIUrl":"10.1007/s00604-026-08389-6","url":null,"abstract":"<div><p>Acute kidney injury (AKI) is a serious clinical condition with high morbidity and mortality, yet early detection and effective treatment remain challenging. Herein, we developed ultrasmall quantum dots (QDs) for dual-modal imaging-guided early detection and antioxidant therapy of AKI. Benefiting from the ultrasmall hydrodynamic size, the as‑prepared Ag₂Se@Mn QDs exhibit favorable renal clearance and prolonged retention in injured kidneys. The QDs possess concentration-dependent T1-weighted magnetic resonance imaging (MRI) contrast and near-infrared fluorescence properties, enabling real-time, non-invasive visualization of cisplatin-induced AKI progression. Especially, MRI revealed renal dysfunction up to 66 h earlier than conventional clinical indicators, providing a critical window for early intervention. Furthermore, the inherent ROS-scavenging activity of Mn²⁺ endows QDs with potent antioxidant capacity by efficiently eliminating hydroxyl radicals (·OH) and superoxide anions (O₂^•-), and thus comprehensively alleviate renal damage and recover renal functions without obvious side-effects. This “all-in-one” theranostic strategy offers an alternative approach for precision management of AKI.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148904932","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A coumarin-quinoline-derived fast and highly sensitive turn on fluorescent probe for sniffing sulfite in food, living cells and zebrafish","authors":"Xuegang Xu,&nbsp;Jiaxin Hong,&nbsp;Huiquan Xiao,&nbsp;Xixiang Xiao,&nbsp;Wuying Yang,&nbsp;Luying Duan,&nbsp;Haoye Lu,&nbsp;Xiaoli Yin,&nbsp;Yanping Hong","doi":"10.1007/s00604-026-08387-8","DOIUrl":"10.1007/s00604-026-08387-8","url":null,"abstract":"<div><p>Building on the precursor probe <b>BMQ-1</b> with A−π−A configuration, a new optimized fluorescent probe, <b>XYT</b>, for the determination of sulfur dioxide was developed by extending the π-conjugation through replacing the electron acceptor 1-(4-dimethylaminophenyl)-2-cyanoacrylonitrile with 1-(7-diethylaminocoumarin-3-yl)-2-cyanoacrylonitrile. This modification resulted in a redshift of <b>XYT</b> in emission wavelength from 590 nm to 603 nm upon reaction with sulfite. The probe demonstrated rapid colorimetric and fluorogenic changes (≈ 10 s), with the solution turning from colorless to red and emitting strong red fluorescence. <b>XYT</b> exhibited high selectivity, photostability, and sensitivity, achieving a detection limit as low as 49.9 nM. In practical detection scenarios, <b>XYT</b> was adopted to measure residual SO₂ contents in commercially available food samples, among which the analytical data of some specimens were verified via comparative analysis with the official Chinese national standard GB 5009.34–2022. Beyond in vitro assays on food matrices, <b>XYT</b> also enables fluorescence imaging of exogenous and endogenous SO₂ in live cells and zebrafish. These properties suggest that <b>XYT</b> holds substantial potential for pathological studies and clinical diagnostics involving SO₂.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148904972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Sm-MOF@Ag electrochemiluminescence immunosensor mediated by CuBi2O4@PDA dual-mode quenching for sensitive detection of anti-Müllerian hormone","authors":"Xuemin Qin,&nbsp;Xiangfei Zheng,&nbsp;Xuyang Deng,&nbsp;Zhi Luo,&nbsp;Shenglan Hu,&nbsp;Jing Li,&nbsp;Biyang Deng","doi":"10.1007/s00604-026-08385-w","DOIUrl":"10.1007/s00604-026-08385-w","url":null,"abstract":"<div><p>By exploiting the dual-quenching effect of CuBi₂O₄@PDA on Sm-MOF@Ag, we developed a signal-on/signal-off electrochemiluminescence (ECL) immunosensor for the sensitive detection of anti-Müllerian hormone (AMH). A novel samarium-based metal-organic framework (Sm-MOF) was employed as the luminophore, with K₂S₂O₈ used as the co-reactant to generate a stable ECL signal. Silver nanoparticles were in situ decorated on the Sm-MOF surface to act as co-reactant accelerators, promoting SO₄^•− generation and significantly enhancing the ECL intensity. For signal quenching, energy transfer from the Sm-MOF@Ag donor to the CuBi₂O₄ acceptor was rationally designed based on their matched energy levels, resulting in pronounced attenuation of the ECL emission. Meanwhile, the polydopamine shell further improved the quenching efficiency by scavenging SO₄^•− radicals. Under optimized conditions, the proposed ECL immunosensor exhibited a wide linear range from 10 fg·mL^− 1 to 100 ng·mL^− 1 and a detection limit of 2.6 fg·mL^− 1. This dual-quenching strategy represents a novel method for the detection of AMH, with substantial potential for implementation in clinical diagnostics.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148904956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}