Microchimica Acta最新文献

{"title":"A rare multi-emission metal–organic complex fluorescent probe for direct oxytetracycline recognition","authors":"Zhaojing Yuan,&nbsp;Long Yu,&nbsp;Wenyuan Jiang,&nbsp;Xin Li,&nbsp;Zhiqing Wen,&nbsp;Xiangyang Hao,&nbsp;Mingtai Sun,&nbsp;Suhua Wang","doi":"10.1007/s00604-025-07160-7","DOIUrl":"10.1007/s00604-025-07160-7","url":null,"abstract":"<div><p>A simple and effective metal–organic coordination polymer, EuIn@MOCPs, which enables the rapid and selective detection of oxytetracycline (OTC) among tetracycline antibiotics was successfully synthesized. Unlike the previously reported rare-earth-doped metal–organic complexes, this probe not only exhibits the common 617-nm characteristic peak in response to OTC but also uniquely generates uncommon peak shifts at 591 nm and 652 nm, allowing it to specifically recognize OTC among tetracycline antibiotics. We found that the response of the probe and OTC had a linear relationship with a detection limit as low as 42.3 nM within the 0–90-μM concentration range using multi-peak ratio fluorescence testing. Finally, the rich color change from blue to red in fluorescence makes this probe an excellent candidate for the development of high-performance visual fluorescent test strips. This achievement provides an effective approach for fluorescent probes to recognize structurally similar contaminants.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of polydentate phosphonate-functionalized adsorbent for selective extraction of rare earth ions in harsh acidic solution","authors":"Xue Chen,&nbsp;Chenyang Wang,&nbsp;Mao Li,&nbsp;Dexiu Zhang,&nbsp;Yinmao Wei","doi":"10.1007/s00604-025-07162-5","DOIUrl":"10.1007/s00604-025-07162-5","url":null,"abstract":"<div><p>Developing adsorbents capable of extracting rare earth element (REE) in harsh acidic solution is a challenging issue in the recycle of REE from secondary resources. In this work, porous polystyrene resin was facile modified with tris(2-aminoethyl)amine followed by methylenebis(phosphonic dichloride) to create a polydentate phosphonate-functionalized adsorbent for this purpose. The adsorption properties of four REE ions (Ce³⁺, Nd³⁺, Gd³⁺, and Dy³⁺) were investigated in terms of affinity constants, selectivity, and recovery. The adsorption mechanism of adsorbent for REE ions is regarded as the strong chelation interaction provided by the phosphonate ligand, which was verified by the experiments of acid and salt effects, thermodynamic and kinetic analysis. By using the diluted digest solution of a permanent magnet as model of strong acidic sample (1 mol/L HCl), the batch adsorption method generates high recovery ranging from 58.1 to 100% toward five REE ions (Y³⁺, Ce³⁺, Nd³⁺, Gd³⁺, and Dy³⁺), while the simulated continuous column bed enhances their total contents from 35.4% in the original digest solution to 88.9% in the eluent. In conclusion, the adsorbent indicates an excellent extraction ability in harsh acidic solution, and the recycle of REE using this adsorbent can predictably reduce alkali consumption to neutralize digest solution of secondary resources and improve greenness in manufacture.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic innovation: MOF@GCN hybrid for electrochemical detection of flutamide—bridging experimental, computational, and real-world applications","authors":"Sirisha Subbareddy,&nbsp;Arehalli Shivamurthy Santhosh,&nbsp;Sahana Kamanna Metry,&nbsp;Kumar Venkatesan,&nbsp;Manickam Selvaraj,&nbsp;Srujan Basavapura Ravikumar,&nbsp;Sandeep Shadakshari","doi":"10.1007/s00604-025-07164-3","DOIUrl":"10.1007/s00604-025-07164-3","url":null,"abstract":"<div><p>Electrochemical sensors are at the forefront of analytical technology, offering remarkable sensitivity and rapid response for detecting a wide range of chemical and biological compounds. Herein, a bimetallic metal–organic framework (MOF) is engineered and combined with graphitic carbon nitride (GCN) to demonstrate exceptional electrochemical performance toward the anti-cancer drug flutamide. A simple solvothermal method is used to synthesize MOF and GCN. These materials are then used as precursors to synthesize the MOF@GCN nanocomposite via a sonication method. The formation of the nanocomposite is confirmed using various characterization techniques like UV–Vis spectroscopy, FTIR spectroscopy, XRD, XPS, TGA, SEM, and TEM. The electrochemical characterization is performed using EIS, and the electrochemical measurements are conducted using CV and LSV. The results obtained from the electrochemical parameters indicate good operational stability, high sensitivity, reliability, and excellent electrochemical conductivity. The LSV curves show linearity over a wide range of flutamide concentration levels (10 to 180 nM), a limit of detection of 17.56 nM, a limit of quantification of 53.23 nM, and an optimal sensitivity of 22.89 µA µM⁻¹ cm⁻². This electrical response of the sensor is attributed to the abundance of active sites, accelerated diffusion, and low rate of recombination. The real sample analysis conducted in biofluids and environmental samples also demonstrate good recovery for the flutamide analyte. The theoretical results obtained from the computational DFT analysis on the analyte are also in good agreement with the experimental results. In a wider perspective, the development of this electrochemical sensor promises significant advancements in health monitoring and environmental protection.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnology-enhanced immunoassay for accurate determination of HT-2 toxin in edible insect samples","authors":"Raúl Sancho-García,&nbsp;Fernando Navarro-Villoslada,&nbsp;Fernando Pradanas-González,&nbsp;Henri O. Arola,&nbsp;Bettina Glahn-Martínez,&nbsp;Tarja K. Nevanen,&nbsp;Elena Benito-Peña","doi":"10.1007/s00604-025-07146-5","DOIUrl":"10.1007/s00604-025-07146-5","url":null,"abstract":"<div><p>Consumption of edible insects is common in non-Western countries of Africa, Asia, Oceania, and Latin America. However, their consumption has significantly increased in Europe in recent years thanks to their remarkable nutritional properties. Edible insects provide a valuable source of high-quality proteins, fats, minerals, and vitamins. Nevertheless, the absence of global regulatory guidelines poses a risk associated with their consumption due to the potential presence of pathogens and contaminants, such as mycotoxins, which are toxic compounds produced by fungi and represent a major threat to food and feed safety. Our approach integrates advanced nanobiotechnology to develop fluorescent antibodies by conjugating a recombinant superfolder green fluorescent protein (sfGFP) with a single-chain antibody (scFv). This fusion allows for precise detection of the immune complex formed between the HT-2 toxin and a biotinylated anti-HT-2 antibody. Additionally, we employed advanced computational tools, including AlphaFold and MOE, to deepen our understanding of the binding interactions present in the immune complex, confirming the strong interaction between the Fab/HT-2 toxin immunocomplex and the scFv antibody fragment, in contrast to the weaker binding observed with the Fab/T-2 toxin and the scFv. The method demonstrates high sensitivity, with an EC₅₀ of 10.3 ± 0.6 ng mL⁻¹, a dynamic range of 3.4 ± 0.1 to 31 ± 3 ng mL⁻¹, a limit of detection of 0.43 ng mL⁻¹, and a limit of quantification of 1.2 ng mL⁻¹ in buffer solution. The assay exhibited excellent precision, with a reproducibility of 4% and no cross-reactivity with other mycotoxins. Application to contaminated cricket flour yielded recoveries between 91 and 133%, with coefficients of variation from 6 to 13%. These results indicate that the developed immunoassay is highly sensitive, selective, and reliable for detecting HT-2 toxin in food matrices, providing a promising tool for mycotoxin screening in food safety.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Silicon carbide and zirconium nitride-based surface plasmon resonance sensors for detecting Serratia marcescens and Micrococcus lysodeikticus","authors":"Chou-Yi Hsu,&nbsp;Prakash Kanjariya,&nbsp;I. A. Ariffin,&nbsp;Asha Rajiv,&nbsp;Aditya Kashyap,&nbsp;G. D. Anbarasi Jebaselvi,&nbsp;Satish Choudhury,&nbsp;Yashpal Yadav,&nbsp;P. Sankara Rao,&nbsp;Sanjeev Kumar Shah,&nbsp;Amrindra Pal","doi":"10.1007/s00604-025-07161-6","DOIUrl":"10.1007/s00604-025-07161-6","url":null,"abstract":"<div><p>A novel surface plasmon resonance (SPR) biosensor design for point-of-care detection of different bacteria is presented. It consists of a SiO₂ prism, metal (Ag), silicon carbide (SiC), 2D materials of zirconium nitride (ZrN), and a sensing medium. The proposed structure’s angular reflectivity is investigated using the transfer matrix method (TMM) following optimization of the Ag and SiC layer thicknesses. For the various types of bacteria such as <i>Staphylococcus (S) aureus</i>, <i>faecalis 9790</i>, <i>aureus Duncan</i>, and <i>aureus 52A5</i>, the maximal sensitivity of 327, 362.45, 301.46, and 269.87°/RIU is achieved with remarkable minimum reflectance (<i>R</i>_min). According to simulation results, using a new class of 2D materials significantly improves the sensor performance over the conventional SPR configuration. Furthermore, the proposed SPR structure is presented with COMSOL Multiphysics to measure the electric field enhancement factor and intensity close to the ZrN material-sensing layer interface. Using the fabrication technologies to fabricate the proposed sensor as an SPR chip is worthwhile due to its real-time and label-free detection of malaria diseases. </p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triggering cascade detection of rutin and Al3+ via 3D ratiometric fluorescent probe through lateral flow assay installation based on dual-emission carbon dots","authors":"Yihao Zhang,&nbsp;Haoming Xing,&nbsp;Houwen Hu,&nbsp;Sineng Gao,&nbsp;Xinru Liu,&nbsp;Linfan Wang,&nbsp;Ting Zhang,&nbsp;Xuan Leng,&nbsp;Da Chen","doi":"10.1007/s00604-025-07154-5","DOIUrl":"10.1007/s00604-025-07154-5","url":null,"abstract":"<div><p>The persistent existence of flavonoid medications and hazardous ion residues continue to be a concern. Herein, a novel 3D cascade nitrogen-doped carbon dots (N-CDs) based sensing platform for specific detection of rutin and Al³⁺ has been developed, utilizing 3,3-diaminobenzidine and catechol as precursors through solvothermal way. The N-CDs exhibit a blue emission peak at 410 nm and a green emission peak at 518 nm. Upon the introduction of rutin, the signal value at 410 nm is significantly reduced by strong inner filter effect (IFE) and weak fluorescence resonance energy transfer (FRET) while the signal value at 518 nm is marginally quenched by weak IFE. Then, with the addition of Al³⁺, the rutin-Al³⁺ complex formed causes the decrease of the signal value at 410 nm by strong FRET and weak IFE while the signal value at 518 nm is decreased by strong IFE. Correspondingly, based on the 3D fluorescence excitation-emission matrix, a novel cascade fluorescent sensor is designed, which shows a good linear relationship with the concentration of rutin and Al³⁺ within the ranges 0.059–35 μM and 0.098–20 μM, featuring limits of detection (LOD) around 59 nM and 98 nM, respectively. In addition, a fluorescence sensing platform based on the lateral flow assay installation has been developed for portable and on-site detection of rutin and Al³⁺. This work not only broadens the application scope of CDs in sensing fields but also offers a highly effective approach for creating a superior fluorescent sensor to detect rutin and Al³⁺, ultimately enhancing capabilities in environmental monitoring.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A ratiometric ionic liquids-based micro-optode for instant and specific detection of nerve agent analog","authors":"Najmin Tohora,&nbsp;Sabbir Ahamed,&nbsp;Jyoti Chourasia,&nbsp;Upika Darnal,&nbsp;Subekchha Pradhan,&nbsp;Shraddha Rai,&nbsp;Shubham Lama,&nbsp;Sudhir Kumar Das","doi":"10.1007/s00604-025-07153-6","DOIUrl":"10.1007/s00604-025-07153-6","url":null,"abstract":"<div><p>Designing advanced photofunctional materials within the core of ionic liquids (ILs) has stimulated considerable attention within the scientific communities due to their impactful significance and physicochemical properties such as ionic nature, low melting point, non-volatility, and tunability without hampering their inherent photofunctionality. Herein, we have synthesized a photoluminescent IL, <b>HTIL</b> from 8-hydroxy pyrene-1,3,6-trisulfonic acid trisodium salt [HPTS] and trihexyltetradecylphosphonium chloride ([TTP]Cl) by simple ionic exchange reaction. Water-dispersible IL-based low-dimensional materials referred to as <b>nHTIL</b> were developed by a reprecipitation technique and validated using various spectroscopic and microscopic analyses methods. We have successfully demonstrated that neat <b>HTIL</b> could be used as a solvent-free fluorescent ink and checked its superiority as a security writing tool. A ratiometric cyanish to bluish fluorometric change is observed upon the addition of diethylchlorophosphate (DCP), a sarin mimic in the solution having detection and quantification limits within the µM range. Also, a portable and super handy paper-based test kit experiment has been illustrated and performed verifying DCP detection in the solid phase. A vapor strip–based experiment by <b>nHTIL</b> was conducted to explore the vapor phase detection of DCP. This report presents an innovative way to develop DCP-sensitive IL-based low-dimensional materials exhibiting remarkable properties compared to traditional ones for forensic and environmental monitoring.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel electrochemical sensor based on MIP technology for sensitive determination of cinacalcet hydrochloride in tablet dosage form and serum samples","authors":"Ipek Kucuk,&nbsp;Selenay Sadak,&nbsp;Selda Zengin Kurnalı,&nbsp;Sacide Altınöz,&nbsp;Bengi Uslu","doi":"10.1007/s00604-025-07152-7","DOIUrl":"10.1007/s00604-025-07152-7","url":null,"abstract":"<div><p>Cinacalcet hydrochloride (CIN) is a calcium-sensing receptor agonist used to treat hypercalcemia in the parathyroid. The molecularly imprinted polymer (MIP)–based sensor (CIN@MIP/GCE) was electropolymerized using cyclic voltammetry (CV) of the functional monomer o-phenylenediamine (o–PD) with a template molecule CIN on a glassy carbon electrode (GCE). The optimum performance of the MIP-based electrode for CIN detection was obtained with parameters of a 1:7 monomer ratio, a 15-min removal time, ethanol as a removal solution, and a 15-min rebinding time. The surface characterization of the CIN@MIP/GCE sensor was conducted using atomic force microscopy (AFM) and scanning electron microscopy (SEM), while CV and electrochemical impedance spectroscopy (EIS) were employed for electrochemical characterization with [Fe(CN)₆]³⁻/⁴⁻ redox probe. AFM findings show that the MIP sensor with CIN-specific voids on the surface has a root-mean-square (RMS) value of 27.95, while the non-imprinted polymer (NIP) sensor without voids has a smoother surface formation and an RMS value of 21.30 nm. The analytical efficacy of the constructed sensor was assessed using differential pulse voltammetry (DPV). The limit of detection (LOD) was 0.17 × 10⁻¹², with a linear range of 1.0 × 10⁻¹²–1.0 × 10⁻¹¹ M. The reliability of the constructed sensor was determined using CIN detection in real samples as tablet dosage form and human serum, yielding recovery results of 100.19% and 101.82%, respectively. The selectivity investigation was performed against prevalent interference substances. The relative imprinting factor (IF) values of CIN impurities confirmed the selectivity of the CIN sensor.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07152-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143835641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aptamer-regulated colorimetric and electrochemical dual-mode sensor for the detection of uranyl ions utilizing AuNCs@COF composite","authors":"Zhijun Chen,&nbsp;Jinquan Liu,&nbsp;Wenyu Wang,&nbsp;Guoqing Qin,&nbsp;Siru Liu,&nbsp;Weilin Zhang,&nbsp;Changmin Peng,&nbsp;Yan Tan,&nbsp;Zhongran Dai,&nbsp;Deshuai Zhen,&nbsp;Le Li","doi":"10.1007/s00604-025-07156-3","DOIUrl":"10.1007/s00604-025-07156-3","url":null,"abstract":"<div><p>Uranium is the core material for the development of the nuclear industry, but its irreversible radiation damage poses a significant threat to human health. In this context, an innovative dual-mode colorimetric and electrochemical sensor was developed for the detection of uranyl ions (UO₂²⁺), utilizing a covalent organic framework@gold nanoclusters (AuNCs@COF) composite. The synthesis of AuNCs@COF was simple, and the incorporation of AuNCs imparted the composite with exceptional peroxidase-like catalytic activity and enhanced electrochemical properties. By regulating the adsorption and desorption of aptamers on the AuNCs@COF surface, both peroxidase-like activity and conductivity were modulated, enabling the detection of UO₂²⁺ utilizing colorimetric and electrochemical dual signals. Under optimal conditions, the sensor revealed a broad linear detection range and a low detection limit, with ranges of 1.36 × 10^–10—1.36 × 10^–5 mol/L for colorimetric detection and 5.0 × 10^–10—2.5 × 10^–5 mol/L for electrochemical detection, achieving detection limits for these two methods of 107 pmol/L and 347 pmol/L, respectively. Unlike other single-mode sensors for UO₂²⁺ detection, this dual-mode sensor demonstrated superior sensitivity, specificity, and repeatability. Furthermore, the results of spiked recovery experiments in real water samples highlight the promising potential of this dual-mode sensor for environmental water monitoring applications.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143826636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical detection of S. typhimurium based on peroxidase-like activity of gold nanoparticle-doped CuZr-MOF nanozyme","authors":"Xiaodong Cao,&nbsp;Chenlu Zhang,&nbsp;Yunan Xu,&nbsp;Wei Wang,&nbsp;Huiying Hu,&nbsp;Keji Chen,&nbsp;Jing Yang,&nbsp;Shudong He,&nbsp;Hanju Sun,&nbsp;Yongkang Ye","doi":"10.1007/s00604-025-07163-4","DOIUrl":"10.1007/s00604-025-07163-4","url":null,"abstract":"<div><p>An electrochemical biosensor using gold nanoparticles (AuNPs)-doped bimetallic-organic framework (BMOF) with enhanced peroxidase-like activity was constructed to detect <i>Salmonella Typhimurium</i> (<i>S. typhimurium</i>). The BMOF of CuZr-MOF was synthesized via a two-step method and used as carrier to in situ immobilize AuNPs. Due to the stability of Zr-MOF, the good electrocatalytic ability of Cu (II), and the synergetic effects of AuNPs, Cu (II) and Zr (IV), the prepared AuNPs@CuZr-MOF nanozyme showed improved stability and catalytic activity to H₂O₂ oxidation. The oxidation reaction was found to be a surface-controlled process of electron transfer and a pH-dependent electron transfer process of oxidation reaction involving two electrons. Further, AuNPs@CuZr-MOF was biofunctionalized with signal DNA probe, forming sDNA-AuNPs@CuZr-MOF nanotags. The biosensing platform was constructed on a glassy carbon electrode modified sequentially with electrodeposited AuNPs, capture DNA probe (cDNA), and BSA. Finally, a sandwich-type detection structure was formed by hybridization reactions between cDNA and target <i>invA</i> gene of <i>S. typhimurium</i>, as well as between <i>invA</i> gene and the sDNA of sDNA@AuNPs@CuZr-MOF nanotags. Under optimized experimental conditions, the biosensor achieved a linear range of 1 × 10⁻¹⁶ to 1 × 10⁻⁸ mol L⁻¹ for the target <i>invA</i> gene with a detection limit (LOD) of 6.2 × 10⁻¹⁷ mol L⁻¹ using differential pulse voltammetry measurement (DPV). It was successfully applied to the direct and quantitative detection of <i>invA</i> gene segments in total DNA extracts of <i>S. typhimurium</i>, showing a linear range from 3.5 to 3.5 × 10⁶ CFU mL⁻¹ and a LOD of 0.82 CFU mL⁻¹. The fabricated biosensor exhibited good selectivity, reproducibility, and storage stability, enabling its use for the detection of <i>invA</i> gene segments in contaminated milk, with recoveries between 95.9% and 103.1%.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143830660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}