Microchimica ActaPub Date : 2025-03-25DOI: 10.1007/s00604-025-07100-5
C. Dhanush, M. Ismayati, M. G. Sethuraman
{"title":"Targeted bio-imaging in discriminating normal and cancerous cells using dual-doped carbon dots derived from Dahlia pinnata flower extract","authors":"C. Dhanush, M. Ismayati, M. G. Sethuraman","doi":"10.1007/s00604-025-07100-5","DOIUrl":"10.1007/s00604-025-07100-5","url":null,"abstract":"<div><p>This research delves into utilizing dual-doped (N & S) carbon dots (DDCDs) sourced from <i>Dahlia pinnata</i> flower extract for targeted bio-imaging, aiming to differentiate between normal and cancerous cells. The synthesized DDCDs, incorporating nitrogen and sulfur, exhibited unique optical, structural, and morphological properties with an average size of about 3.25 nm. These DDCDs demonstrate strong fluorescence and display excitation-dependent emission behavior, as confirmed by the results of photoluminescence spectroscopy. Folic acid conjugation with DDCDs enhances their specificity towards cancer cells expressing the folate receptor. Through comprehensive characterization, the study demonstrates the successful synthesis and functionalization of these DDCDs. Even at a concentration of 200 µg/ml, these DDCDs demonstrated low cytotoxicity. In vitro experiments on both normal and cancer cell lines reveal distinct fluorescence responses, showcasing the potential of these bio-compatible DDCDs for precise bio-imaging in cancer diagnostics. This work opens avenues for utilizing natural sources in nanomaterial synthesis for biomedical applications, contributing to the advancement of targeted cellular imaging technologies.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143698598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A rapid on-site fluorescence sensing platform for malachite green in an aqueous phase based on lanthanide-functionalized MOF","authors":"Yu Yan, Hao Guo, Zhiguo Yu, Zeyun Yang, Dengke Zhuang, Yanyan Ma, Mingyue Wang, Wu Yang","doi":"10.1007/s00604-025-07113-0","DOIUrl":"10.1007/s00604-025-07113-0","url":null,"abstract":"<div><p>The residue of malachite green (MG) in water has serious toxic effects on the human body, so it is very important to establish a highly sensitive and convenient method for MG detection. In this study, a novel double-emission lanthanide MOF fluorescence probe (Eu-Zn-MOF) was designed for monitoring MG in water by post-synthetic modification (PSM) to Zn-MOF synthesized by a simple hydrothermal method. Eu-Zn-MOF exhibits excellent photoluminescence properties, making it suitable as a ratiometric fluorescence sensor (<i>I</i><sub>410</sub>/<i>I</i><sub>615</sub>) for the sensitive detection of MG. MG effectively quenches the luminescence of Eu<sup>3+</sup> at 615 nm, while the luminescence of the Zn-MOF frame at 410 nm remains unaltered. The fluorescence sensing platform had a relatively wide linear range (0–30 µM), low detection limit (9.8 nM), high sensitivity, and rapid response time (within 1 min). At the same time, the recovery of actual samples was 90.2–107.0%, and the relative standard deviation (RSD) was ≤ 3.82%. Simultaneously, by combining the smartphone color recognition APP with RGB analysis, a portable smartphone auxiliary sensing platform was established to realize the visual detection of MG. This method provides a novel strategy for real-time monitoring of pollutants in the environment.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143698599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microchimica ActaPub Date : 2025-03-25DOI: 10.1007/s00604-025-07105-0
Dandan Jiang, Yangyang Li, Siyu Wu, Lan Lan, Jinghai Liu
{"title":"A cerium phthalocyanine-based covalent organic framework-magnetic core–shell composite as efficient affinity material for the enrichment of phosphopeptides","authors":"Dandan Jiang, Yangyang Li, Siyu Wu, Lan Lan, Jinghai Liu","doi":"10.1007/s00604-025-07105-0","DOIUrl":"10.1007/s00604-025-07105-0","url":null,"abstract":"<div><p> A novel cerium phthalocyanine-based covalent organic framework (CePc-COF)-magnetic core–shell composite was fabricated by grafting the CePc-COF layer on the surface of Fe<sub>3</sub>O<sub>4</sub>. The obtained core–shell composite particle (Fe<sub>3</sub>O<sub>4</sub>@CePc-COF) had strong magnetic responsiveness (29.6 emu g<sup>−1</sup>) and good hydrophilicity (5.0°). The affinity material provided abundant metal ion sites for specific enrichment of phosphopeptides. Fe<sub>3</sub>O<sub>4</sub>@CePc-COF had good performance in terms of high selectivity (1: 1: 5000), sensitivity (0.1 fmol), recovery (92.91%), and reusability (10 cycles). The enrichment feasibility of Fe<sub>3</sub>O<sub>4</sub>@CePc-COF was first investigated in standard peptides. Furthermore, Fe<sub>3</sub>O<sub>4</sub>@CePc-COF can efficiently identify phosphopeptides from extremely complex samples. The work can provide a novel idea for the fabrication of metallophthalocyanine based-COF materials for phosproteome detection in biological samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143688500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microchimica ActaPub Date : 2025-03-24DOI: 10.1007/s00604-025-07034-y
Seyed Ali Akbar Sajadi, Zeynab Khorablou
{"title":"Poly-taurine/poly-L-glutamic acid double-layer coating as potential candidates for surface modification of carbon felt electrode for discrimination and simultaneous detection of morphine and tramadol","authors":"Seyed Ali Akbar Sajadi, Zeynab Khorablou","doi":"10.1007/s00604-025-07034-y","DOIUrl":"10.1007/s00604-025-07034-y","url":null,"abstract":"<div><p>An ultrasensitive and reliable electrochemical scaffold was designed for the individual and simultaneous measurement of morphine (Mor) and tramadol (Trm) addictive and illegal drugs, utilizing a cost-effective and flexible carbon felt electrode modified with double-layer poly-taurine/poly-L-glutamic acid (P(Tau)/P(Glu)/CF). It is worth noting that drugs have now become a part of daily life in all societies, and the consumption of tranquilizers and opiates such as Mor and Trm has also increased. Given the frequent co-use of Mor and Trm, accurate and reliable methods for their simultaneous measurement are crucial. Simultaneous diagnostics make the determination more efficient and cost-effective by reducing the need for multiple sensors. Surface modification of CFE was carried out by a green approach, facile and straightforward route by layer-by-layer electropolymerization, forming a thin polymeric film with abundant functional groups responsible for anchoring narcotic drugs. The P(Tau)/P(Glu)/CFE composite showed an exceptionally high rate of active site exposure and proper electrochemical activity, attributed to the synergistic effects of the constituent materials. P(Tau)/P(Glu)/CFE was successfully used to detect saliva, urine, plasma, and body sweat samples with satisfactory recoveries.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143688323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Trimetallic PtPdCo nanoalloy on hollow porous N-doped carbon fibers: A high-performance electrochemical immunosensor for procalcitonin detection in SIRS diagnosis","authors":"Yan-Yu Shi, Yu-Lin Zhong, Ai-Jun Wang, Li-Ping Mei, Pei Song, Tiejun Zhao, Jiu-Ju Feng","doi":"10.1007/s00604-025-07089-x","DOIUrl":"10.1007/s00604-025-07089-x","url":null,"abstract":"<div><p>Systemic inflammatory response syndrome (SIRS) poses a serious threat that influences patient survival and life quality. Its early and accurate diagnosis is paramount in clinics, where procalcitonin (PCT) is recognized as a key serological biomarker. Herein, well-dispersed PtPdCo nanoalloy was efficiently anchored on hollow porous N-doped carbon fibers (termed PtPdCo HPCNFs) through a sequential process involving electrospinning, pyrolysis, etching, and co-reduction. The morphology, structure, and physicochemical properties of PtPdCo HPCNFs were characterized by a set of techniques. Subsequently, the synthesized PtPdCo HPCNF nanocomposite was applied to build a label-free electrochemical amperometric immunosensor for ultrasensitive detection of PCT. Its performance was evaluated by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). Notably, the biosensor demonstrated a wide dynamic linear range (0.0001–1000 ng mL<sup>−1</sup>) and a low detection limit (0.20 pg mL<sup>−1</sup>). Furthermore, the sensor achieved the bioassay of PCT in clinical samples, yielding acceptable results. This work offers a promising approach for synthesizing superior nanomaterials for bioanalytical applications, highlighting their potential in development of sensing devices, especially for early and sensitive diagnosis of SIRS through PCT detection.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Highly sensitive electrochemical determination of cariprazine using a novel Ti3C2@CoAl2O4 nanocomposite: application to pharmaceutical and biological sample analysis","authors":"Elif Naz Öven, Asena Ayse Genc, Nevin Erk, Wiem Bouali, Qamar Salamat, Mustafa Soylak","doi":"10.1007/s00604-025-07104-1","DOIUrl":"10.1007/s00604-025-07104-1","url":null,"abstract":"<div><p>Cariprazine (CAR) is an atypical antipsychotic drug used for the treatment of schizophrenia and bipolar disorder. This study presents the development of a novel, highly sensitive electrochemical sensor based on a Ti<sub>3</sub>C<sub>2</sub>@CoAl<sub>2</sub>O<sub>2</sub> nanocomposite–modified glassy carbon electrode (GCE) for the detection of CAR in pharmaceutical and biological samples. The innovative Ti<sub>3</sub>C<sub>2</sub>@CoAl<sub>2</sub>O<sub>2</sub> composite, synthesized and characterized through Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy coupled with energy-dispersive spectroscopy, and thermogravimetric analysis, revealed exceptional structural integrity, morphology, composition, and thermal stability. The electrochemical properties of the modified electrode were evaluated using cyclic voltammetry and electrochemical impedance spectroscopy, demonstrating enhanced conductivity, an increased electroactive surface area, and reduced charge transfer resistance compared to the bare GCE. Differential pulse voltammetry was employed for CAR detection under optimized conditions, yielding a linear range of 0.2–5.6 μM with a regression equation <i>I</i><sub>pa</sub> (μA) = 0.133 <i>C</i><sub>CAR</sub> (μM) + 0.09 (<i>R</i><sup>2</sup> = 0.993). The limit of detection and limit of quantification were determined as 0.02 µM and 0.07 µM, respectively, highlighting the sensor’s high sensitivity. The modified electrode exhibited excellent repeatability with a relative standard deviation (RSD) of = 2.9% and reproducibility (RSD = 2.8%), along with strong selectivity against common interfering substances. The sensor was successfully applied to human blood serum, urine, and CAR tablets, achieving high recovery values (98.52–103.94%), confirming its reliability for real-sample analysis. These findings underline the novelty and potential of the Ti<sub>3</sub>C<sub>2</sub>@CoAl<sub>2</sub>O<sub>2</sub>-modified GCE as a powerful tool for the accurate, selective, and sensitive determination of CAR in clinical and pharmaceutical applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07104-1.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143676466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The development and application of SERS-based lateral flow immunochromatography in the field of food safety","authors":"Wenxi Liu, Hao Wang, Wenhui Zhong, Yichun Zhang, Yingyue Liu, Xibao Gao, Mengmeng Yan, Chao Zhu","doi":"10.1007/s00604-025-07047-7","DOIUrl":"10.1007/s00604-025-07047-7","url":null,"abstract":"<div><p>Surface-Enhanced Raman Scattering-Lateral Flow Immunoassay (SERS-LFIA) inherits the advantages of simplicity, rapidness, and stability from Lateral Flow Immunoassay (LFIA), while integrating the sensitivity and accuracy of SERS, thereby attracting extensive attention in the field of food safety monitoring. This paper delves into the design strategies and principles underlying SERS-LFIA, introducing the detection formats based on SERS and contrasting the differences between traditional Raman molecules and those located in the Raman-silent region. It analyzes two immunoassay methods, namely sandwich and competitive, along with their respective applications. Importantly, by reviewing the applications of SERS-LFIA in food safety monitoring over the past 5 years, this paper summarizes the challenges faced by SERS-LFIA technology in practical applications and development. Furthermore, it provides a forward-looking perspective on the future development of SERS-LFIA. As a pivotal analytical method in the field of food safety monitoring, SERS-LFIA is demonstrating immense potential. It is hoped that this paper will offer valuable insights for the future development and application of SERS-LFIA.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07047-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143668278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Quantitative immunosensor for dibenz[a,h]anthracene on-site detection in oilfield chemicals based on computer-aided antibody","authors":"Jiaxun Li, Haifeng Chen, Yong Shu, Luming Jiang, Wei Gao, Hua Kuang, Chuanlai Xu, Lingling Guo","doi":"10.1007/s00604-025-07035-x","DOIUrl":"10.1007/s00604-025-07035-x","url":null,"abstract":"<div><p> A paper sensor, a gold nanoparticles-based lateral flow immunochromatographic assay (GNPs-LFIA), was successfully established for the rapid quantitative detection of dibenz[a,h]anthracene (DBA) in drilling fluids (DFs). Computational analysis was employed to rationally design a novel hapten to effectively expose the active site of DBA, resulting in the successful development of a monoclonal antibody with high sensitivity and specificity. The half-maximum inhibitory concentration was 5.814 ng/mL. Then, the GNPs-LFIA was established following the optimization of the extraction agent and method. The limit of detection for DF samples was 0.273 mg/kg. Recovery experiments showed a high level of consistency with the results obtained by high-performance liquid chromatography-fluorescence detection, which indicated that the established GNPs-LFIA offered exceptional accuracy and reliability. Consequently, this method is well-suited for the rapid screening and determination of DBA in oilfield chemicals and presents a technical solution to identify polycyclic aromatic hydrocarbons.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microchimica ActaPub Date : 2025-03-20DOI: 10.1007/s00604-025-07099-9
Saisai Zhang, Yi Zheng, Bo Zhang, Bowen Zhang, Na Luo, Yan Wang
{"title":"Construction of heterojunctions of In2O3 nanocube with NiO for rapid detection of carbon monoxide","authors":"Saisai Zhang, Yi Zheng, Bo Zhang, Bowen Zhang, Na Luo, Yan Wang","doi":"10.1007/s00604-025-07099-9","DOIUrl":"10.1007/s00604-025-07099-9","url":null,"abstract":"<div><p>Aimed at realizing the rapid and effective detection of carbon monoxide (CO) in high humidity environments, the NiO-modified In<sub>2</sub>O<sub>3</sub> nanocube with different NiO loadings (1, 3, 5 mol%) was synthesized via a two-step method. Morphological characterizations revealed that the NiO modification did not alter the cubic morphology of In<sub>2</sub>O<sub>3</sub>, and the nanocube showed a porous structure with pore sizes of around 10 nm. The XPS analysis evidenced that the 3 mol% NiO/In<sub>2</sub>O<sub>3</sub> sample owns more Ov contents (40.62%) than that of the pure In<sub>2</sub>O<sub>3</sub> sample (31.73%). The gas sensing measurements demonstrated that the 3 mol% NiO/In<sub>2</sub>O<sub>3</sub> sensor exhibited a decreased optimal operating temperature of 260℃ (300℃ for In<sub>2</sub>O<sub>3</sub>) and good stability. Compared with pristine In<sub>2</sub>O<sub>3</sub>, the 3 mol% NiO/In<sub>2</sub>O<sub>3</sub> nanocube showed an enhanced response of 4.16 (2.73 for In<sub>2</sub>O<sub>3</sub>) to 500 ppm CO and a rapid response/recovery time (10 s/13 s) toward CO. Furthermore, the 3 mol% NiO/In<sub>2</sub>O<sub>3</sub> sensor exhibited superior humidity resistance, enabling accurate CO detection even at 85% relative humidity. The enhanced gas sensing performance of the NiO/In<sub>2</sub>O<sub>3</sub> nanocube is attributed to the unique porous cubic structure and the formation of p-n heterojunctions. This work demonstrates a viable strategy to improve the CO sensing capabilities of In<sub>2</sub>O<sub>3</sub> by constructing NiO/In<sub>2</sub>O<sub>3</sub> heterostructures.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microchimica ActaPub Date : 2025-03-19DOI: 10.1007/s00604-025-07021-3
Andrea Cabrero-Martín, Sara Santiago, Verónica Serafín, María Pedrero, Ana Montero-Calle, José M. Pingarrón, Rodrigo Barderas, Susana Campuzano
{"title":"Multifunctional cerium nanolabels in electrochemical immunosensing with improved robustness and performance: determination of TIM-1 in colorectal cancer scenarios as a case study","authors":"Andrea Cabrero-Martín, Sara Santiago, Verónica Serafín, María Pedrero, Ana Montero-Calle, José M. Pingarrón, Rodrigo Barderas, Susana Campuzano","doi":"10.1007/s00604-025-07021-3","DOIUrl":"10.1007/s00604-025-07021-3","url":null,"abstract":"<div><p>A multifunctional cerium oxide nanoparticles (CeO<sub>2</sub>NPs)-based nanolabel is exploited to implement an electrochemical sandwich-type immunoplatform for the determination of T-cell immunoglobulin and mucin domain 1 (TIM-1) biomarker, a mucin-like class I membrane glycoprotein associated with cancer angiogenesis. The immunoplatform is constructed using screen-printed electrodes where capture antibody is immobilized through the chemistry of diazonium salts. CeO<sub>2</sub>NPs exhibit robust pseudo-peroxidase activity even at high substrate concentrations. They are covalently functionalized in a simple manner after carboxylation with a detector antibody (dAb), acting dually as a nanozyme and nanocarrier for sensing bioreceptors. This allows the development of immunoplatforms with improved robustness and performance (in terms of a moderate enhancement in sensitivity, a significant expansion in the linear range, and a reduction in the background current) compared with the immunoplatforms prepared using nanolabels also decorated with the natural enzyme (horseradish peroxidase, HRP) or the conventional enzymatic labeling involving the dAb and an HRP-secondary antibody. Under the optimized experimental conditions, the developed electrochemical immunoplatform allows the highly sensitive detection of the TIM-1 glycoprotein, with a detection limit of 9.9 pg mL<sup>−1</sup> and a linear working range of 33–600 pg mL<sup>−1</sup>. This performance permits biomarker quantification within clinically relevant ranges. This innovative configuration enables the precise diagnosis and stratification of colorectal cancer patients by analyzing plasma samples without pretreatment beyond a sample dilution and allows establishment of the first cut-off values reported for this purpose.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143655217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}