Microchimica Acta最新文献_第8页

A sensitive bromate sensor based on a gold nanoparticle-poly(diallyldimethylammonium chloride)–reduced graphene oxide nanocomposite modified glassy carbon electrode 基于金纳米粒子-聚二烯基二甲基氯化铵-还原氧化石墨烯纳米复合材料修饰玻碳电极的灵敏溴酸盐传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-30 DOI: 10.1007/s00604-024-06871-7

Ai-ping Zhang, Yi-xuan Fan, Ning Wang, Hao Yu

{"title":"A sensitive bromate sensor based on a gold nanoparticle-poly(diallyldimethylammonium chloride)–reduced graphene oxide nanocomposite modified glassy carbon electrode","authors":"Ai-ping Zhang, Yi-xuan Fan, Ning Wang, Hao Yu","doi":"10.1007/s00604-024-06871-7","DOIUrl":"10.1007/s00604-024-06871-7","url":null,"abstract":"<div><p>A nanocomposite consisting of gold nanoparticles (AuNPs), poly(diallyldimethylammonium chloride) (PDDA), and reduced graphene oxide (rGO) was fabricated by a two-step chemical reduction method. Firstly, a PDDA-rGO composite was prepared by using hydrazine hydrate as a reducing agent. Subsequently, the AuNP-PDDA-rGO composite was prepared in ethylene glycol with PDDA-rGO and HAuCl<sub>4</sub> as raw materials using sodium citrate as a reduction agent. The resulting composite was characterized using X-ray powder diffraction (XRD), Raman spectroscopy, transmission electron microscopy (TEM), and electrochemical methods. This composite was then modified on a glassy carbon electrode (GCE) by the dropping method. The electrochemical behavior of bromate on this modified electrode was investigated. The results showed that PDDA-rGO can be used as a good carrier to obtain AuNPs with small particle sizes and good dispersion. The AuNPs and PDDA-rGO in composite enhanced the electrochemical activity of the electrode. Under the synergistic action of each component, the resulting electrode exhibited high activity for the electrochemical reduction of bromate. Based on this, an amperometric bromate sensor was fabricated in N<sub>2</sub>-saturated 0.10 mol/L HCl with attractive features including a wide linear range of 1.0 × 10<sup>−7</sup>–1.7 × 10<sup>−3</sup> mol/L, a low detection limit (3<i>s</i><sub>b</sub>) of 3.2 × 10<sup>−8</sup> mol/L, and a high sensitivity of 2317 µA/mM/cm<sup>2</sup>. The sensor was successively used for the determination of bromate in drinking water.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid evaluation of hepatocellular carcinoma by detecting plasma exosomes with time-resolved fluorescence immunochromatographic test strips 用时间分辨荧光免疫层析试纸条检测血浆外泌体对肝癌的快速评价

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-28 DOI: 10.1007/s00604-024-06903-2

Jiaming Li, Jianfen Su, Minghui Li, Yaofen Wu, Huiqiang Chen, Xihua Fu, Hongliang Yao, Jinping Chen, Yuntao Liu, Jie Zan

{"title":"Rapid evaluation of hepatocellular carcinoma by detecting plasma exosomes with time-resolved fluorescence immunochromatographic test strips","authors":"Jiaming Li, Jianfen Su, Minghui Li, Yaofen Wu, Huiqiang Chen, Xihua Fu, Hongliang Yao, Jinping Chen, Yuntao Liu, Jie Zan","doi":"10.1007/s00604-024-06903-2","DOIUrl":"10.1007/s00604-024-06903-2","url":null,"abstract":"<div><p>Time-resolved fluorescence immunochromatographic test strips (TRFIS) was developed for the rapid detection of hepatocellular carcinoma (HCC)-specific plasma exosomes (hExos) by targeting the hExo-surface membrane protein glypican-3 (GPC3). The GPC3-TRFIS could directly detect plasma exosomes without the isolation and purification process, and the whole immunoassay could be completed within 15 min. The visual detection limit of GPC3-TRFIS was 3.44 × 10^9 particles/mL, with a minimum detection limit of 1.8 × 10^9 particles/mL. For analysis of the clinical HCC samples, GPC3-TRFIS shows high specificity for detection of hExo, and was nearly unreactive for healthy donors’ samples. GPC3-TRFIS was able to efficiently distinguish HCC patients (19 cases) from healthy donors (19 cases). Overall, the developed TRFIS offers the benefits of high sensitivity, simple operation, and no need of large precision instruments and professional technical personnel for rapid detection of plasma hExos, and supplies a novel approach for early screening of HCC.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrafine metal–organic framework @ graphitic carbon with MoS2-CNTs nanocomposites as carbon-based electrochemical sensor for ultrasensitive detection of catechin in beverages 超细金属-有机框架@石墨碳与MoS2-CNTs纳米复合材料作为碳基电化学传感器用于饮料中儿茶素的超灵敏检测

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-28 DOI: 10.1007/s00604-024-06901-4

Yilin Wang, Zixia Zhao, Yuefan Wang, Zhifang Liu, Leyao Chen, Jin Qi, Yixi Xie, Pengcheng Zhao, Junjie Fei

{"title":"Ultrafine metal–organic framework @ graphitic carbon with MoS2-CNTs nanocomposites as carbon-based electrochemical sensor for ultrasensitive detection of catechin in beverages","authors":"Yilin Wang, Zixia Zhao, Yuefan Wang, Zhifang Liu, Leyao Chen, Jin Qi, Yixi Xie, Pengcheng Zhao, Junjie Fei","doi":"10.1007/s00604-024-06901-4","DOIUrl":"10.1007/s00604-024-06901-4","url":null,"abstract":"<div><p>GO/Co-MOF/PPy-350 (GPC-350) was synthesized by in situ growth of ultrafine Co-MOF on graphene oxide (GO), followed by encapsulation with polypyrrole (PPy) and calcination at 350.0℃. Meanwhile, MoS<sub>2</sub>-MWCNTs (MoS<sub>2</sub>-CNTs) were produced via the in situ synthesis of MoS<sub>2</sub> within multi-walled carbon nanotubes (MWCNTs). The electrochemically superior GPC-350/MoS<sub>2</sub>-CNTs nanocomposite was then achieved by combining GPC-350 with MoS<sub>2</sub>-CNTs. The polypyrrole encapsulation serves to protect the ultrafine Co-MOF, preventing its degradation during the calcination process. The linear detection range of the GPC-350/MoS<sub>2</sub>-CNTs/GCE sensor for the determination of catechin (CA) in phosphate buffered saline (PBS) was from 5.0 to 1800.0 nM with a limit of detection of 1.78 nM. In addition, the materials were characterized using SEM, EDX, TEM, XRD, EIS, XPS, FTIR, and Raman. These results indicate that the synthesis of GPC-350/MoS<sub>2</sub>-CNTs nanocomposites is successful and CA in beverages samples can be effectively detected using electrochemical sensors. Additionally, the reaction mechanism of CA was explored through cyclic voltammetry. The application of GPC-350/MoS<sub>2</sub>-CNTs nanocomposites in sensor technology offers innovative approaches for the ultrasensitive detection of flavonoids.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of colorimetric-fluorescent dual-signal aptamer-based assay using COF-Au nanozyme and magnetic nanoparticle–based CdTe quantum dots for sensitive zearalenone determination 基于COF-Au纳米酶和磁性纳米粒子CdTe量子点的比色-荧光双信号配体灵敏测定法的构建

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-28 DOI: 10.1007/s00604-024-06914-z

Xi Ma, Minyi Hui, Jiayu Yuan, Zhouping Wang, Xiaoyuan Ma

{"title":"Construction of colorimetric-fluorescent dual-signal aptamer-based assay using COF-Au nanozyme and magnetic nanoparticle–based CdTe quantum dots for sensitive zearalenone determination","authors":"Xi Ma, Minyi Hui, Jiayu Yuan, Zhouping Wang, Xiaoyuan Ma","doi":"10.1007/s00604-024-06914-z","DOIUrl":"10.1007/s00604-024-06914-z","url":null,"abstract":"<div><p>A dual-signal aptamer-based assay utilizing colorimetric and fluorescence techniques was developed for the determination of zearalenone (ZEN). The CdTe quantum dots, serving as the fluorescent signal source, were surface-modified onto Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> and subsequently functionalized with the aptamer. The COF-Au was modified with complementary chain, which possessed peroxide (POD)-like enzyme properties, and could catalyze the peroxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to ox TMB, resulting in the generation of colorimetric signals. The two parts were merged based on the principle of base complementary pairing, resulting in an assembled structure exhibiting a diminished fluorescence signal due to the Förster resonance energy transfer (FRET) effect. Due to the higher affinity of the aptamer towards the target, the presence of ZEN resulted in the detachment of COF-Au, leading to an increase in supernatant concentration of COF-Au proportional to ZEN concentration. Consequently, this enhanced the catalytic ability and amplified the colorimetric signal. The fluorescence of precipitation increased simultaneously with the reduction of FRET, enabling linear detection of colorimetry in the range 0.5 ~ 10,000 μg·kg<sup>−1</sup> and fluorescence in the range 0.1 ~ 10,000 μg·kg<sup>−1</sup>, with respective detection limits of 0.36 μg·kg<sup>−1</sup> and 0.09 μg·kg<sup>−1</sup>. The spike recovery in wheat flour and corn ranged from 93.4 to 122.0%. This technology was simple to operate and had low cost and good application prospects.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel electrochemical immunosensor for the ultrasensitive detection of vascular endothelial growth factor based on MB@Cu-MOFs/MWCNTs composite 一种基于MB@Cu-MOFs/MWCNTs复合材料的超灵敏血管内皮生长因子检测电化学免疫传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-28 DOI: 10.1007/s00604-024-06884-2

Junjie Liu, Shengxi Wu, Lu Zhang, Mingjun Zhang, Haotian Wu, Miwan Shi, Yuan Weng, Wenqiao Ye, Kui Gu

{"title":"A novel electrochemical immunosensor for the ultrasensitive detection of vascular endothelial growth factor based on MB@Cu-MOFs/MWCNTs composite","authors":"Junjie Liu, Shengxi Wu, Lu Zhang, Mingjun Zhang, Haotian Wu, Miwan Shi, Yuan Weng, Wenqiao Ye, Kui Gu","doi":"10.1007/s00604-024-06884-2","DOIUrl":"10.1007/s00604-024-06884-2","url":null,"abstract":"<div><p>A novel proposal is introduced with an unlabeled electrochemical immunosensor for the detection of tumor broad-spectrum biomarker vascular endothelial growth factor (VEGF165) Copper-based metal organic frameworks (Cu MOFs)-carbon nanotubes (MWCNTs) were employed as its substrates, functionalized with methylene blue (MB) for signal enhancement. Cu-MOFs-MWCNTs nanocomposites were synthesized successfully via a solvothermal method and were then deposited on the surface of a glassy carbon electrode (GCE), with the addition of methylene blue to amplify the signal. Due to the expansive specific surface area provided by the carbon nanotubes and the amino groups facilitated by the metal–organic framework nanomaterials, the anti-VEGF165 monoclonal antibody was immobilized on the electrochemical immunosensor through covalent bonding, which could bind specifically to VEGF165, thereby causing a detectable change in the current. The developed immunosensor exhibited a linear detection range for VEGF165 spanning from 1 to 100,000 pg/ml, with the <i>R</i><sup>2</sup> value of 0.997 by differential pulse voltammetry (DPV) method, and the limit of detection was as low as 0.9686. Additionally, the electrochemical immunosensor presented commendable selectivity, availability, and reproducibility, along with an effective capacity for detecting VEGF165 in clinical samples. Consequently, the immunosensor is expected to revolutionize early screening for VEGF165-related diseases, which offer a promising, cost-effective, and precise diagnostic tool for clinical utility.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142889859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A colorimetric sensor for the sensitive and rapid detection of ampicillin based on CS-Cu,Fe/HS nanozyme 基于CS-Cu，Fe/HS纳米酶的氨苄西林灵敏快速比色传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-27 DOI: 10.1007/s00604-024-06895-z

Linqian Hou, Jinya Wei, Chen Xiang, Dezhi Yang, Yaling Yang

{"title":"A colorimetric sensor for the sensitive and rapid detection of ampicillin based on CS-Cu,Fe/HS nanozyme","authors":"Linqian Hou, Jinya Wei, Chen Xiang, Dezhi Yang, Yaling Yang","doi":"10.1007/s00604-024-06895-z","DOIUrl":"10.1007/s00604-024-06895-z","url":null,"abstract":"<div><p>A novel copper and iron doped containing chitosan and heparin sodium carbon dots (CS-Cu,Fe/HS) nanozyme was formulated through a single-step microwave digestion method. CS-Cu,Fe/HS exhibits excellent peroxidase (POD)-like activity and positive charge characteristics, and it can oxidize the negatively charged 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) in the presence of H<sub>2</sub>O<sub>2</sub> to produce a green compound (ox-ABTS). Furthermore, CS-Cu,Fe/HS enhances electron transfer and provides additional active sites through the valence state transformations of Fe<sup>2+</sup>/Fe<sup>3+</sup> and Cu<sup>2+</sup>/Cu<sup>+</sup>. Interestingly, the POD-like activity of CS-Cu,Fe/HS is inhibited with the introduction of ampicillin (AMP), which may be because the Cu and Fe ions in CS-Cu,Fe/HS form complexes with AMP, leading to changes in the structure or surface properties of the nanozyme, thereby reducing the number of active sites on the nanozyme. Drawing from this, a straightforward and reliable colorimetric sensor was constructed for AMP detection, featuring a linear range of 0.033 to 110 μg/mL and a detection limit as low as 11.6 ng/mL. The proposed detection method for AMP performed well in real samples, with recoveries ranging from 94.8% to 110.2%.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142889473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon-based light addressable potential aptasensor based on the synergy of C-MXene@rGO and OPD@NGQDs for low-density lipoprotein detection 基于C-MXene@rGO和OPD@NGQDs协同作用的碳基光可寻址电位适体传感器用于低密度脂蛋白检测

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-27 DOI: 10.1007/s00604-024-06909-w

Guiyin Li, Guangxiong Wu, Qing Huang, Shuaikang Dong, Yu Zhou, Mei Lu, Jintao Liang, Xueqing Zhou, Zhide Zhou

{"title":"Carbon-based light addressable potential aptasensor based on the synergy of C-MXene@rGO and OPD@NGQDs for low-density lipoprotein detection","authors":"Guiyin Li, Guangxiong Wu, Qing Huang, Shuaikang Dong, Yu Zhou, Mei Lu, Jintao Liang, Xueqing Zhou, Zhide Zhou","doi":"10.1007/s00604-024-06909-w","DOIUrl":"10.1007/s00604-024-06909-w","url":null,"abstract":"<div><p>A novel carbon-based light-addressable potentiometric aptasensor (C-LAPS) was constructed for detection low-density lipoprotein (LDL) in serum. Carboxylated Ti<sub>3</sub>C<sub>2</sub> MXene @reduced graphene oxide (C-MXene@rGO) was used as interface and o-phenylenediamine functionalized nitrogen-doped graphene quantum dots (OPD@NGQDs) as the photoelectric conversion element. The photosensitive layers composed of OPD@NGQDs/C-MXene@rGO exhibit superior photoelectric conversion efficiency and excellent biocompatibility, which contribute to an improved response signal. When LDL reacts with the LDL aptamer (LDL<sub>Apt</sub>) immobilized on the photosensitive layers to form LDL-LDL<sub>Apt</sub> complexes, the reaction process can induce the modification of the surface potential in the photosensitive layer, leading to potential shift observed through the I-V curves. The experimental conditions were successfully optimized with few planned tests by applying the Box-Behnken design and response surface methodology aspects of the Design-Expert software. Under the optimal condition, the potential shift had a linear relationship with concentrations of LDL from 0.02 to 0.30 μg/mL. The limit of detection (LOD) was 5.88 ng/mL (S/N = 3) and the sensitivity was 315.20 mV/μg·mL<sup>−1</sup>. In addition, the LDL C-LAPS demonstrated excellent specificity, reproducibility, and stability in detecting LDL. The sensor performed well in quantifying LDL in real samples. Therefore, the LDL C-LAPS has the potential for clinical applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142889998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Silver nanowire/gold nanosphere binary plasma-assembled membranes for sensitive SERS detection of homocysteine 银纳米线/金纳米球二元等离子体组装膜用于灵敏的SERS检测同型半胱氨酸

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-26 DOI: 10.1007/s00604-024-06891-3

Shirun Peng, Yujun Zheng, Wanjing Li, Minghuan Lin, Zhihong Wu, Ruiyun You, Qingqiang Lin, Yaling Wu

{"title":"Silver nanowire/gold nanosphere binary plasma-assembled membranes for sensitive SERS detection of homocysteine","authors":"Shirun Peng, Yujun Zheng, Wanjing Li, Minghuan Lin, Zhihong Wu, Ruiyun You, Qingqiang Lin, Yaling Wu","doi":"10.1007/s00604-024-06891-3","DOIUrl":"10.1007/s00604-024-06891-3","url":null,"abstract":"<div><p> Silver nanowire (Ag NW)/gold nanosphere (Au NS) binary plasma films were prepared using plasma coupling between Ag NWs and Au NSs. The plasma films formed by combining these two noble metals showed better sensitivity for SERS detection with a minimum detection concentration of 10<sup>−8</sup> M for R6G compared to pure Ag NWs or Au NSs. After rational optimisation of the substrate preparation process, the substrate showed good homogeneity, reproducibility and stability. We perform an indirect detection of homocysteine (Hcy) through a specific reaction of Hcy with o-phthalaldehyde (OPA). The lowest detectable concentration of Hcy was 5 × 10<sup>−9</sup> M. The recoveries of Hcy were 94.53 ~ 103.43% with the relative standard deviations (RSDs) of 2.53%, 9.21% and 12.26%, respectively, in the spike recovery experiments. With good selectivity and accuracy for Hcy detection, this plasma film provides an idea for the detection of disease markers in serum.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient electrochemiluminescence sensor utilizing Zr-PyTCPPMOF for swift hydrogen sulfide detection 利用Zr-PyTCPPMOF快速检测硫化氢的高效电化学发光传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-26 DOI: 10.1007/s00604-024-06878-0

Xueke Chen, Tongfu Huang, Xin Wang, Chunyan Wu, Fei Yi, Ziqiang Wan, Bao Zou, Wei Xiong, Fusheng Liao, Hao Fan, Zhaojiang Yin

{"title":"Efficient electrochemiluminescence sensor utilizing Zr-PyTCPPMOF for swift hydrogen sulfide detection","authors":"Xueke Chen, Tongfu Huang, Xin Wang, Chunyan Wu, Fei Yi, Ziqiang Wan, Bao Zou, Wei Xiong, Fusheng Liao, Hao Fan, Zhaojiang Yin","doi":"10.1007/s00604-024-06878-0","DOIUrl":"10.1007/s00604-024-06878-0","url":null,"abstract":"<div><p> The ultrahigh-sensitive detection of H<sub>2</sub>S is reported using a novel dual-ligand metal–organic framework (MOF) electrochemiluminescence (ECL) sensor. By combining tetrakis(4-carboxyphenyl) porphyrin (TCPP) and 1,3,6,8-tetrakis(4-carboxyphenyl) pyrene (TBAPy) as ligands and employing zirconium as the metal source, a spindle-shaped Zr-PyTCPPMOF was successfully designed and synthesized. Notably, the multiple nitrogen structures of porphyrin provided abundant binding sites for sulfur (S), further enhancing the ECL signal of Zr-PyTCPPMOF. The sensor shows a good linear relationship in the 0.01–100 μM range, with a detection limit reaching 1.18 nM (S/N = 3). When analyzing actual serum samples, the recovery was between 96.6 and 106.2%. Ultimately, the dual-ligand ECL platform based on Zr-PyTCPPMOF achieved trace detection of H<sub>2</sub>S, which is expected to provide reliable technical support for early environmental monitoring and disease diagnosis.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06878-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effective multicolor visual biosensor for ochratoxin A detection enabled by DNAzyme catalysis and gold nanorod etching 利用DNAzyme催化和金纳米棒刻蚀技术制备了一种有效的赭曲霉毒素A多色视觉生物传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-26 DOI: 10.1007/s00604-024-06883-3

Zhiqiang Guo, Mingshuo Zhang, Haiping Zhang, Xinru Ren, Yijing Xiao, Weiqing Sun, Yu Wang, Su Liu, Jiadong Huang

{"title":"Effective multicolor visual biosensor for ochratoxin A detection enabled by DNAzyme catalysis and gold nanorod etching","authors":"Zhiqiang Guo, Mingshuo Zhang, Haiping Zhang, Xinru Ren, Yijing Xiao, Weiqing Sun, Yu Wang, Su Liu, Jiadong Huang","doi":"10.1007/s00604-024-06883-3","DOIUrl":"10.1007/s00604-024-06883-3","url":null,"abstract":"<div><p> A novel detection technique is introduced that offers sensitive and reliable ochratoxin A (OTA) detection. The method leverages the etching of gold nanorods (AuNRs) stabilized by hexadecyl trimethyl ammonium bromide (CTAB) using the oxidized form of 3,3′,5,5′-tetramethyl benzidine sulfate (TMB<sup>2+</sup>), creating a susceptible multicolor visual detection system for OTA. The visual detection is enabled by Mg<sup>2+</sup>-assisted DNAzyme catalysis combined with the catalytic hairpin assembly (CHA) signal amplification strategy. The presence of OTA triggers CHA and signaling responses along with the formation of G-quadruplex-hemin DNAzyme, which promotes the oxidation of TMB with H<sub>2</sub>O<sub>2</sub>, leading to the etching of AuNRs and a reduction in their aspect ratio. AuNRs experienced a blue shift in the longitudinal localized surface plasmon resonance peak, resulting in a color change. The technique has been shown to detect OTA with a low detection limit of 0.309 pg/mL, demonstrating high sensitivity and specificity. The detection technique offers versatility by enabling the detection of other pollutants through a simple replacement of the aptamer, expanding the range of detection platforms available for pollutant determinations.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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