Microchimica Acta最新文献

{"title":"Trimethylamine N-oxide detection for early prediction of renal function utilizing a three-dimensional localized electronic structure (3DLES) biosensor","authors":"Wei-Cheng Lin,&nbsp;Wei-Lun Yen,&nbsp;Yun-Yu Hsieh,&nbsp;Bing-Hong Chen,&nbsp;Yi-Huan Chang,&nbsp;Tzu-Wei Chung","doi":"10.1007/s00604-025-07556-5","DOIUrl":"10.1007/s00604-025-07556-5","url":null,"abstract":"<div><p> A novel Micro-Electro-Mechanical Systems (MEMS) is introduced based biosensor architecture employing a three-dimensional localized electronic structure (3DLES) array capable of detecting trimethylamine N-oxide, (TMAO, (CH₃)₃NO), concentrations as low as 0.2 μM in biological fluids such as urine or serum. The design incorporates a modified Cole–Cole model, wherein newly introduced parameters for the proposed 3DLES array biosensor are able to quantify enzymatic impedance effects. These variables offer insight into redox behavior and the fine-scale electrical currents generated by catalytic activity. On-chip signal processing is incorporated into the system, enabling fast detection within 1 s and Yielding a high sensitivity of 320 ADC units per micromolar (equivalent to 5.5 mV/μM). Very high repetition (98.1%) and low signal drift (0.4 mV over time) further demonstrate the system’s reliability. TMAO detection is facilitated through minute variations in capacitive properties induced by the TorA enzyme, Yielding a detectable differential response of 10.6%. Comparison with traditional cyclic voltammetry (CV) shows excellent agreement, with only 0.024% deviation between methodologies. The 3DLES biosensor also exhibits a high TMAO-to-TMA conversion efficiency (88%) and impressive selectivity (97%) for the target analyte, making it a viable candidate for early-stage renal function assessment in non-clinical settings. The strong correlation between the proposed biosensor and mass spectrometry results across 100 urine samples (<i>R</i>² = 0.954), along with the extracted linear equation <i>Y</i> = 120.9 − 39.2 × <i>X</i> (where <i>Y</i> is the ADC count of TMAO and <i>X</i> is the UACR), highlights the biosensor’s reliability and effectiveness in quantifying renal function biomarkers. This compact and cost-effective device offers a promising pathway toward at-home renal function pre-screening through metabolic profiling.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated sampling and in situ SERS detection of diquat and paraquat using silver nanostars-modified PTFE substrates","authors":"Hui Pan,&nbsp;Meng Jin,&nbsp;Hua Shao,&nbsp;Xiao Meng,&nbsp;Haiyan Wei,&nbsp;Xinyuan Zhao,&nbsp;Ge Gao,&nbsp;Wei Zhang,&nbsp;Cuijuan Wang","doi":"10.1007/s00604-025-07555-6","DOIUrl":"10.1007/s00604-025-07555-6","url":null,"abstract":"<div><p> A surface-enhanced Raman spectroscopy (SERS) strategy for integrated sampling and in situ detection of diquat (DQ) and paraquat (PQ) in air and water is presented. By leveraging the unique characteristics of the SERS fingerprint alongside the exceptional adsorption capacity of porous polytetrafluoroethylene (PTFE) membranes, we synthesized a composite substrate consisting of porous PTFE and silver nanostars (AgNSts). Utilizing the AgNSts@PTFE filter, DQ and PQ can be sampled with an air sampler and detected in situ using a portable Raman spectrometer effectively, without pre-processing steps. Strong correlations were found between SERS intensity and logarithmic analyte concentration, with R² values between 0.937 and 0.968. The detection limits for DQ and PQ were 0.74 ppb and 0.23 ppb, respectively, and are below the permissible concentrations. This study presents a novel, rapid method for detecting DQ and PQ in aerosols in non-laboratory settings, offering an alternative approach to conventional techniques.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145231106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel MOF combined with dummy molecularly imprinted polymer paper-based colorimetric sensor equipped with a smartphone detector for sensitive, portable, and on-site detection of organophosphorus pesticides","authors":"Yuqing Wang,&nbsp;Jiaxin Chen,&nbsp;Yutong Liu,&nbsp;Ruizhen Zhang,&nbsp;Jing Hong,&nbsp;Yunli Zhao","doi":"10.1007/s00604-025-07552-9","DOIUrl":"10.1007/s00604-025-07552-9","url":null,"abstract":"<div><p> A novel paper-based colorimetric sensor was synthesized based on a core–shell metal–organic framework (MOF) coated with dummy molecularly imprinted polymer (DMIP) for detecting organophosphorus pesticides (OPs). The MOF, used as the core, enhances sensor sensitivity owing to its inherent advantages such as high catalytic activity and stability; meanwhile, the introduction of MIP effectively overcomes the limitation of MOFs in single-substrate detection. This sensor shows great on-site detection potential and superior analytical performance owing to the combination of color response and quantitative analysis via a smartphone. Six OPs were successfully detected in agricultural products with a limit of detection as low as 0.5 ng/mL. Moreover, the consistency between the sensor and HPLC–MS/MS results was verified, yielding satisfactory agreement ranging from 94.4% to 100%. In conclusion, this method is promising for portable, rapid, and on-site food safety detection.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrophoresis-based electrochemical sensor for rapid detection of sialic acid in human serum","authors":"Xiaoqing Ming,&nbsp;Zhanghong Wu,&nbsp;Shuluo Yin,&nbsp;Xiangyu Zuo,&nbsp;Qing Lu,&nbsp;Xiaoting Wu,&nbsp;Xinhang Li,&nbsp;Shengnan Tang,&nbsp;Fangxin Liu,&nbsp;Yuanfang Hou,&nbsp;Jianjun Hu,&nbsp;Guangchao Zang","doi":"10.1007/s00604-025-07551-w","DOIUrl":"10.1007/s00604-025-07551-w","url":null,"abstract":"<div><p> A novel electrochemical sensor is introduced that significantly accelerates sialic acid (SA) detection by utilizing an electrophoresis device, which reduces the binding time from 20 to 5 min. The results indicate that the current signal change (ΔI) of the sensor is more than twofold higher than that of conventional sensors after 5 min. Furthermore, to enhance the intensity of the sensor’s signal and simplify the operational procedure, we employed a method that electrochemically reduces graphene oxide/3-aminophenylboronic acid (GO/APBA) to reduced graphene oxide/3-aminophenylboronic acid (rGO/APBA) in a single step. This reduction process effectively removes some oxygen groups from GO, further enhancing the current signal. The linear detection range for SA was between 0.1 mM and 3 mM, with a detection limit of 0.084 mM (S/N = 3). Notably, the electrophoresis-enhanced sensor can successfully detect SA in actual human serum samples. Overall, this sensor represents a significant advancement in the electrochemical sensing of SA, providing a rapid, selective, and cost-effective approach for developing electric field-enhanced detection technologies.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145224838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Well-defined high-entropy alloyed nanoplates triggering cocktail effect for dual-mode detection of organophosphorus pesticide","authors":"Jin-Yu Li,&nbsp;Li-Ping Mei,&nbsp;Zhi-Gang Wang,&nbsp;Ke-Ming Fang,&nbsp;Ai-Jun Wang,&nbsp;Jiu-Ju Feng","doi":"10.1007/s00604-025-07580-5","DOIUrl":"10.1007/s00604-025-07580-5","url":null,"abstract":"<div><p> Well-defined high-entropy alloy PtPbBiFeMn nanoplates (HEAPs) were synthesized by a wet-chemical co-reduction approach. The PtPbBiFeMn HEAPs exhibited excellent peroxidase (POD)-like activity. Leveraging this POD-mimicking property, a dual-mode sensor was developed for quantitative determination of a typical organophosphorus (OP) omethoate. Specifically, the nanozyme oxidizes colorless 3,3′,5,5′-tetramethylbenzidine (TMB) and <i>o</i>-phenylenediamine (OPD) with H₂O₂ to its colored form. This reaction is regulated by alkaline phosphatase (ALP)-mediated hydrolysis of ascorbic acid 2-phosphate (AAP) to ascorbic acid (AA), which reduces oxidized TMB and modulates the detection signals. Additionally, ascorbic acid (AA) is converted to dehydroascorbic acid (DHAA). Consequently, an ALP-dependent fluorescence response is obtained through the condensation reaction between OPD and DHAA, yielding the fluorescent compound 3-(1,2dihydroxy ethyl) furo[3,4-<i>b</i>] quinoxalin-1(3 H) with a maximum emission wavelength in the range 568 to 430 nm. When ALP existed, the colorimetric method for omethoate displayed a linear range of 10.02–167.10 × 10⁻⁴ mg kg⁻¹ with a limit of detection (LOD) of 8.76 × 10⁻⁴ mg kg⁻¹ (<i>S</i>/<i>N</i> = 3), while a ratiometric fluorescence (<i>I</i>₄₃₀/<i>I</i>₅₆₈) approach had a linear range of 6.68 × 10⁻⁴–384.3 × 10⁻⁴ mg kg⁻¹ with an LOD of 6.63 × 10⁻⁴ mg kg⁻¹ (<i>S</i>/<i>N</i> = 3). This work provides constructive insights to simplify biosensing platforms for food safety and environmental monitoring.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145210668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel tripod probe and lateral flow test to improve CRISPR/Cas12a assay: benefits of branched probe based on trebler phosphoramidite modification","authors":"Irina V. Safenkova,&nbsp;Maria V. Kamionskaya,&nbsp;Aleksandr V. Ivanov,&nbsp;Anatoly V. Zherdev,&nbsp;Boris B. Dzantiev","doi":"10.1007/s00604-025-07495-1","DOIUrl":"10.1007/s00604-025-07495-1","url":null,"abstract":"<div><p>CRISPR/Cas12a-based assays, when integrated with lateral flow tests (LFTs), provide highly specific nucleic acid detection in a simple, rapid, and equipment-free format. Nevertheless, traditional DNA probes utilized for cleavage by Cas12a have limitations as the cleaved probe only has one label. To overcome this challenge, we engineered a novel type of DNA probe with multiple fluorescein (FAM) labels and a biotin-labeled single-stranded DNA fragment (polyFAM probe). The cleaved polyFAM parts of the probes were detected using a specially designed sandwich LFT, where FAM-specific antibodies were immobilized in the test zone and conjugated with gold nanoparticles. The LFT ensured accurate recognition of the cleaved polyFAM fragments within 10 min. A comparison of five distinct polyFAM probes revealed that the highest signal-to-noise ratio was achieved with a tripod-branched probe synthesized via trebler phosphoramidite modification. Each arm of the tripod probe consists of a hexaethylene glycol spacer ending in a FAM label. Upon Cas12a cleavage, the tripod structure carrying three FAMs is released and detected by LFT. A rapid magnetic separation strategy was subsequently implemented, facilitating the efficient removal of uncleaved probes via biotin–streptavidin capture within 5 min. The CRISPR/Cas12a–tripod–LFT strategy demonstrated excellent sensitivity without preamplification, with a detection Limit of 1.4 pM for DNA target of <i>Salmonella </i>Typhimurium. The CRISPR/Cas12a-tripod-LFT with preliminary loop-mediated isothermal amplification enabled the detection of as few as 0.3 cells per reaction. This innovative tripod probe with corresponding LFT creates a universal, sensitive, rapid, and equipment-free biosensing platform for CRISPR/Cas12a-based diagnostics in point-of-care applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145210669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proof-of-concept study of a CD-type microfluidic titration system with an ISFET sensor for microlitre-scale analysis","authors":"Juntao Yang,&nbsp;Shoji Yamamoto,&nbsp;Kazuhiro Morioka,&nbsp;Akihide Hemmi,&nbsp;Hajime Kayanne,&nbsp;Hizuru Nakajima","doi":"10.1007/s00604-025-07574-3","DOIUrl":"10.1007/s00604-025-07574-3","url":null,"abstract":"<div><p>Conventional titration methods rely on bulky equipment, large sample volumes, and manual reagent handling, limiting their integration into portable and microlitre-scale analytical platforms. This constraint persists despite the growing demand for compact chemical quantification tools in decentralized diagnostics and environmental monitoring. A key challenge remains the lack of precise, automated titration systems that can operate without external pumps, fragile electrodes, or complex tubing. To address this gap, we present a CD-type centrifugal microfluidic device integrated with an ion-sensitive field-effect transistor (ISFET) sensor for automated acid–base titration in microlitre-scale samples. Rotation-driven fluid control is employed in the lab-on-a-disc system, enabling sequential acid addition without external pumps or valves. The 3D-printed microfluidic chip incorporates ten reagent reservoirs connected to an outer reaction chamber, allowing precise titrant delivery through controlled rotational speeds. The ISFET sensor, featuring a Ta₂O₅ sensing membrane and a carbon-based reference electrode, exhibited high sensitivity (51.3 mV/pH) and notable linearity (<i>R</i>² = 0.9929) across a broad pH range. Titration experiments using 300 μL of a sodium carbonate solution and 0.1 mol/L hydrochloric acid successfully revealed equivalence points associated with carbonate neutralization, closely matching the results obtained with a commercial pH meter. These findings validate the measurement reliability and reproducibility of the device and demonstrate its potential as a portable and low-cost titration platform. The proposed approach advances the miniaturization and automation of titration methods, offering promising applications in field-deployable chemical analysis, marine biogeochemistry, and point-of-care diagnostics.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07574-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual signal amplification strategy of Au@ZIF67 catalysis and target cycle amplification for sensitive chemiluminescence detection of adenosine","authors":"Yuanling Sun,&nbsp;Yanan Hou,&nbsp;Xuelei Peng,&nbsp;Jiajia Ye,&nbsp;Chuannan Luo","doi":"10.1007/s00604-025-07572-5","DOIUrl":"10.1007/s00604-025-07572-5","url":null,"abstract":"<div><p>Adenosine (Ade), a crucial potential biomarker in tumors, holds the potential to monitor tumor progression as its levels can be gauged in urine or serum. Herein, a highly sensitive chemiluminescence (CL) aptasensor for Ade detection was developed by leveraging a dual signal amplification strategy that combines the catalysis of Au@ZIF67 and target cyclic amplification. Firstly, Au@ZIF67 was synthesized through the in situ modification of gold nanoparticles onto ZIF67. Concurrently, Fe₃O₄@nSiO₂ was fabricated to serve as a magnetic substrate. Subsequently, the surfaces of these two materials were modified with oligonucleotide chains, yielding Au@ZIF67-ssDNA and Fe₃O₄@nSiO₂/DNAH1, respectively. During the construction of the aptasensor, the two materials were assembled via the complementary base pairing interaction between ssDNA and DNAH1 to form the probe. Upon exposure to Ade, Au@ZIF67-ssDNA was liberated into the solution. Thereafter, the target-stimulated catalytic hairpin self-assembly (CHA) reaction was initiated in the presence of complementary hairpin DNAH2. This led to the formation of Fe₃O₄@nSiO₂-DNAH1/DNAH2, concomitantly releasing Ade once again for cyclic utilization and further augmenting the CL reaction. Therefore, a sensitive and accurate methodology for Ade detection was proposed, underpinned by the dual signal amplification strategy integrating Au@ZIF67 catalysis and target cyclic amplification. Under optimized conditions, the detection limit of Ade reached 1.5 × 10^–12 M, enabling the successful detection of Ade in human urine samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145196239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-driven biosensor for the rapid and selective detection of hypoxia-inducible factor-prolyl hydroxylase domain inhibitors in aqueous media and saliva","authors":"Rebecca L. Houston,&nbsp;Godwin Ayoko,&nbsp;Emad L. Izake","doi":"10.1007/s00604-025-07579-y","DOIUrl":"10.1007/s00604-025-07579-y","url":null,"abstract":"<div><p>Hypoxia-inducible factor-prolyl hydroxylase domain inhibitors (HIF-PHI) are to increase the production of EPO and haemoglobin in patients with chronic kidney disease. HIF inhibitors such as Molidustat and Vadadustat are used as doping agents in racing sports. We present new sensitive and selective materials for the biosensing of Molidustat and Vadadustat in aqueous solution and saliva. Plasmonic nickel foam was synthesised using a reproducible chemical method that deposits forests of closely packed cauliflower-shaped gold nanostructures on the nickel surface. The plasmonic material showed a high enhancement factor of Raman signals (1.09 × 10⁷). Therefore, it was used as a sensor for the detection of HIF inhibitors by surface-enhanced Raman spectroscopy (SERS). A target-specific extractor chip for HIF-PHI was fabricated by functionalising the plasmonic nickel foam with prolyl hydroxylase domain-containing protein 2 (PHD2) molecules. The surface functionalisation was carried out by a green synthesis process that utilises deep UV LED light to convert the PHD2 molecule into a bio-thiol that attaches to the plasmonic nickel foam via Au–S bonds. Using the new SERS sensor and extractor chip, Molidustat and Vadadustat were quantified in aqueous solution down to 0.01 µg/L. The new materials were also utilised for the rapid screening of HIF-PHI in spiked saliva samples. The new materials and light-driven surface functionalisation process can be easily adopted to develop new screening methods for multiple biomolecules (e.g. drugs, proteins, disease biomarkers, environmental toxins) for many applications (e.g. therapeutic drug monitoring, doping control, clinical diagnostics environmental monitoring).</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07579-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145196240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-target electrochemical aptasensor based on Mil101(Fe)-CQD-TiO2 ternary composite for Cryptosporidium and cadmium ion detection","authors":"Indiphile Nompetsheni,&nbsp;Nithyadharsen Palaniyandy,&nbsp;Ntuthuko Wonderboy Hlongwa,&nbsp;Xolile Fuku","doi":"10.1007/s00604-025-07567-2","DOIUrl":"10.1007/s00604-025-07567-2","url":null,"abstract":"<div><p> A Mil101 (Fe)-carbon quantum dot-titanium dioxide (Mil101(Fe)-CQD-TiO₂) ternary composite was prepared using the hydrothermal method. The composite was thoroughly characterized using various physicochemical techniques to investigate the crystallinity of the materials, the functional groups present, chemical vibrations, elemental composition, and morphology, confirming the formation of the ternary composite. The electrochemical properties of the ternary composite were then evaluated through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This ternary composite served as an electrode modifier for aptasensor fabrication. The aptasensor exhibited an electroactive surface area of 3.22 cm², indicating a higher number of active sites for analyte interaction. The aptasensing platform was optimized and evaluated in phosphate buffer solution and real water samples using square wave voltammetry (SWV). Additionally, the sensor demonstrated a limit of detection (LOD) of 0.001 µM for <i>Cryptosporidium</i> and 0.073 µM for cadmium ions with high sensitivities of 9 × 10⁻⁴ mA/µM and 0.127 mA/µM in buffer solutions. The aptasensor demonstrated excellent selectivity, with recoveries ranging from 67 to 98.1% for <i>Cryptosporidium</i> and 72 to 89% for Cd²⁺.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07567-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145205290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}