Microchimica Acta最新文献_第2页

A dual-channel lipid droplet-targeting probe for the detection of polarity and HOCl in models of NAFLD, inflammation, and cancer 一种双通道脂滴靶向探针，用于检测NAFLD、炎症和癌症模型中的极性和HOCl

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-25 DOI: 10.1007/s00604-025-07171-4

Tingting Yang, Shiyu Yin, Huina Liu, Shunyi Wang, Chengqi Wang, Yuxin Chen, Xiaoya Liu, Fangke Zhu, Jiaxuan Guo, Lajiatai, Dongzhi Suonanmu, Qingjia Ren, Shenghua Liao, Caolong Li

{"title":"A dual-channel lipid droplet-targeting probe for the detection of polarity and HOCl in models of NAFLD, inflammation, and cancer","authors":"Tingting Yang, Shiyu Yin, Huina Liu, Shunyi Wang, Chengqi Wang, Yuxin Chen, Xiaoya Liu, Fangke Zhu, Jiaxuan Guo,  Lajiatai, Dongzhi Suonanmu, Qingjia Ren, Shenghua Liao, Caolong Li","doi":"10.1007/s00604-025-07171-4","DOIUrl":"10.1007/s00604-025-07171-4","url":null,"abstract":"<div><p>Abnormal lipid droplet accumulation and metabolism can precipitate various diseases. Understanding the dynamics of intracellular lipid droplets, particularly hypochlorite acid (HOCl) and polarity, is crucial for elucidating the onset and progression of these diseases. In this study, we synthesized a near-infrared fluorescent probe, <b>NSSP</b>, featuring a D-π-A structure, which facilitates the independent dual-response detection of HOCl and polarity. <b>NSSP</b> not only reveals the polarity and HOCl fluctuations in inflammatory cells and inflammation in live mice but also differentiates between normal and fatty liver tissues through two distinct channels. Furthermore, the probe has proven effective in identifying neoplasms within live murine models at both the cellular and systemic levels, thereby affirming its utility as an instrumental resource for the precocious detection of carcinomas. This offers a hopeful avenue for the diagnostic scrutiny of pathologies.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143875464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of octyl-modified silica isoporous membrane for hydrophobic enrichment and electrochemical detection of methylmercury in aquatic products 制备疏水富集和电化学检测水产品中甲基汞的辛基改性二氧化硅异孔膜

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-24 DOI: 10.1007/s00604-025-07169-y

Guiyun Zhang, Zhiwei Lai, Jianhua Xie, Weiqiang Yang

{"title":"Preparation of octyl-modified silica isoporous membrane for hydrophobic enrichment and electrochemical detection of methylmercury in aquatic products","authors":"Guiyun Zhang, Zhiwei Lai, Jianhua Xie, Weiqiang Yang","doi":"10.1007/s00604-025-07169-y","DOIUrl":"10.1007/s00604-025-07169-y","url":null,"abstract":"<div><p>Methylmercury (CH<sub>3</sub>Hg<sup>+</sup>), a lipophilic environmental pollutant, accumulates in fish, shellfish, and other organisms, posing significant risks to human health through the food chain. Developing a convenient and sensitive analytical method for CH<sub>3</sub>Hg<sup>+</sup> detection is crucial for reducing costs and enhancing the efficiency of food safety testing. In this study, we prepared an octyl-modified silica isoporous membrane on the indium tin oxide (ITO) electrode (Octyl-SIM/ITO) via the electrochemical-assisted self-assembly (EASA) method using octyltrimethoxysilane (O-TES) as the functional organosilane. The Octyl-SIM/ITO electrode exhibits vertically-ordered nanochannels and strong hydrophobic affinity, enabling selective penetration and enrichment of weakly polar analytes. Utilizing square wave anodic stripping voltammetry (SWASV), the Octyl-SIM/ITO electrode demonstrates superior electrochemical response signals for CH<sub>3</sub>Hg<sup>+</sup> detection, achieving a detection limit as low as 4 nM. This method allows for accurate and reproducible detection of CH<sub>3</sub>Hg<sup>+</sup> in fish and oyster samples with minimal sample preparation, offering promising potential for portable in situ detection.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143865481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of immobilized xanthine oxidase with magnetic metal–organic framework and its application in screening of active ingredients in traditional Chinese medicine 磁性金属有机骨架固定化黄嘌呤氧化酶的制备及其在中药活性成分筛选中的应用

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-24 DOI: 10.1007/s00604-025-07167-0

Chenxi Wu, Na Zhang, Hailin Li, Hao Wang, Lifeng Han, Yuefei Wang, Fuyi Li, Fei Tian

{"title":"Preparation of immobilized xanthine oxidase with magnetic metal–organic framework and its application in screening of active ingredients in traditional Chinese medicine","authors":"Chenxi Wu, Na Zhang, Hailin Li, Hao Wang, Lifeng Han, Yuefei Wang, Fuyi Li, Fei Tian","doi":"10.1007/s00604-025-07167-0","DOIUrl":"10.1007/s00604-025-07167-0","url":null,"abstract":"<div><p>Enzymes play a crucial role in the development and progression of various diseases, making them important targets for drug development. However, the stability issues associated with natural enzymes limit their broader application. Traditional methods for screening enzyme inhibitors from natural products are often time-consuming and labor-intensive. In this study, we designed and employed magnetic metal–organic frameworks (MOFs) to immobilize xanthine oxidase for the first time. By leveraging the porous structure and high specific surface area of MOFs, combined with the magnetic responsiveness of nanoparticles, we successfully developed a novel method for the efficient screening of potential enzyme inhibitors derived from natural products. By using 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide (EDC/NHS) as a cross-linking agent, we achieved efficient immobilization of xanthine oxidase and identified baicalin as a potential inhibitor from the extract of <i>Scutellaria baicalensis</i>. In addition, we confirmed the adsorption capacity of this method for hordenine, demonstrated the specific adsorption of allopurinol, and also performed in vitro activity validation for baicalein. We not only successfully prepared the immobilized enzyme but also showcased that this method can efficiently screen and isolate potential enzyme inhibitors from traditional Chinese medicine, which provides a rapid and efficient new strategy for identifying enzyme inhibitors in natural products. This innovative approach offers a fresh perspective on the application of botanical medicine and the pharmacological treatment of hyperuricemia, which has important theoretical and practical significance.</p>\u0000<div><figure><div><div><picture><source><img></source></picture></div><div><p>Schematic diagram of synthesis of XO@MMOF (<b>A</b>) and ligand fishing process (<b>B</b>)\u0000</p></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MOF-engineered Cu2O nanozymes with boosted peroxidase-like activity for colorimetric-fluorescent dual-mode detection of deoxynivalenol mof工程的Cu2O纳米酶具有增强过氧化物酶样活性，用于比色-荧光双模式检测脱氧雪腐镰刀菌醇

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-24 DOI: 10.1007/s00604-025-07140-x

Xiaodong Zhu, Yangchun He, Xinhua Xie, Bobo Zhang, Junhao Wang, Haoran Shen, Yingju Liu, Huifu Ji, Hongshuai Zhu

{"title":"MOF-engineered Cu2O nanozymes with boosted peroxidase-like activity for colorimetric-fluorescent dual-mode detection of deoxynivalenol","authors":"Xiaodong Zhu, Yangchun He, Xinhua Xie, Bobo Zhang, Junhao Wang, Haoran Shen, Yingju Liu, Huifu Ji, Hongshuai Zhu","doi":"10.1007/s00604-025-07140-x","DOIUrl":"10.1007/s00604-025-07140-x","url":null,"abstract":"<div><p>The development of a high sensitivity biosensor for the detection of highly toxic deoxynivalenol (DON) is vital for human health and food security. In this work, by integrating metal-organic frameworks (MOF) with cubic Cu<sub>2</sub>O nanoparticles (Cu<sub>2</sub>O@MOF), the nanocomposite achieved a 4.8-fold increase in specific surface area compared to pristine Cu<sub>2</sub>O, which synergistically enhanced its peroxidase-like (POD) activity through optimized substrate affinity and accelerated charge transfer. Consequently, based on the marriage properties of POD activity and fluorescence signal from Cu<sub>2</sub>O@MOF nanoparticles and carbon dots (CDs), a colorimentric-fluorescent dual-mode biosensor was constructed for DON detection. Concurrently, the competitive binding of DON with immobilized antigens on Cu<sub>2</sub>O@MOF-CDs results in antibody displacement, leading to progressive reduction of captured probes with increasing DON concentrations, thereby inducing proportional attenuation in both colorimetric and fluorescence signal intensities. Under the optimum conditions, the established biosensor achieved a detection limit of 0.0018 ng/mL for DON. Furthermore, the prepared dual-mode biosensor was successfully applied to detect DON in tap water, wheat and corn, demonstrating its practical utility for real-world applications. Overall, this work not only advances nanozyme design through MOF-mediated interface engineering but also provides a rapid, accurate, and field-deployable strategy for monitoring mycotoxins in complex matrices.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143871252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Highly sensitive chemiluminometric and colorimetric probes for acetamiprid assay based on Cu2SnS3 quantum dots with peroxide-mimicking activity 校正：基于具有过氧化物模拟活性的Cu2SnS3量子点的高灵敏度化学发光和比色探针

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-23 DOI: 10.1007/s00604-025-07176-z

Yalda Haghighi Shishavan, Mohammad Amjadi

引用次数: 0

A rapid, specific, and simple-to-use biosensor for amplification-free determination of microRNA based on electrical potential-assisted and ternary hybridization 一种基于电势辅助和三元杂交的快速，特异性和简单易用的生物传感器，用于无扩增的microRNA测定

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-22 DOI: 10.1007/s00604-025-07143-8

Yaru Liu, Yang Li, Hao Gong, Yao Liu, Yijie Wang, Cuiping Ma, Yuxi Wei, Chao Shi

{"title":"A rapid, specific, and simple-to-use biosensor for amplification-free determination of microRNA based on electrical potential-assisted and ternary hybridization","authors":"Yaru Liu, Yang Li, Hao Gong, Yao Liu, Yijie Wang, Cuiping Ma, Yuxi Wei, Chao Shi","doi":"10.1007/s00604-025-07143-8","DOIUrl":"10.1007/s00604-025-07143-8","url":null,"abstract":"<div><p> An ultra-fast, easy-to-use and non-amplification electrochemical detection platform was constructed for microRNA (miRNA) detection. A set of label-free hairpin probes and capture probes were introduced to form a ternary complex, which could enhance the selectivity and stability of miRNA detection due to the ability of reducing non-specific bind with non-target and enhancing accessibility of target to probes. Moreover, the capture probe immobilization and hybridization process were accelerated by the external electric field, shortening the detection time from 2 h to 5 min. The platform showed a detection limit of 1.28 fM under ideal experimental control conditions and had ability to identify 1- or 2-nucleotide (nt) difference. In addition, the designed sensor achieved the sensitive determination of miRNA-21 in serum samples. The excellent anti-interference capability of this detection method indicated its potential for clinical application. Its simplicity and high specificity made this method a promising tool for detecting different miRNA to assist the diagnosis of diverse cancers.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ping pong chrysanthemum-like Bi-BiOI and ternary core–shell structured Pd@AuPt based dual-electric signal outputs biosensor for accurate detection of CA19 - 9 基于乒乓型菊花型Bi-BiOI和三元核壳结构Pd@AuPt的双电信号输出生物传感器，用于CA19 - 9的精确检测

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-22 DOI: 10.1007/s00604-025-07150-9

Siyu Wu, Hongyun Ma, Lin Song, Wen Zhong, Yingying Gu, Yuqing Miao, Yarui An

{"title":"Ping pong chrysanthemum-like Bi-BiOI and ternary core–shell structured Pd@AuPt based dual-electric signal outputs biosensor for accurate detection of CA19 - 9","authors":"Siyu Wu, Hongyun Ma, Lin Song, Wen Zhong, Yingying Gu, Yuqing Miao, Yarui An","doi":"10.1007/s00604-025-07150-9","DOIUrl":"10.1007/s00604-025-07150-9","url":null,"abstract":"<div><p>Carbohydrate antigen 19–9 (CA19 - 9) can be used as a biomarker for pancreatic cancer. Measuring the concentration of CA19 - 9 in serum is essential for screening pancreatic cancer patients. In this paper, a dual-electric signal outputs biosensor based on ping pong chrysanthemum-like Bi-BiOI and ternary core–shell structured Pd@AuPt was constructed for ultra-sensitive detection of tumor marker CA19 - 9 using differential pulse voltammetry (DPV) and chronoamperometry (i-t). Ping pong Chrysanthemum-like Bi-BIOI was prepared via one-pot hydrothermal method. To realize the covalent bonding of bismuth-based materials with MWCNT, bismuth-based materials were functionalized by amino groups. MWCNT-NH<sub>2</sub>-Bi-BIOI with large specific surface area and remarkable electrical conductivity was used as the sensing platform. Ternary core–shell structured Pd@AuPt with peroxide-like activity and enhanced biocompatibility immobilized massive antibodies through covalent Au–N and Pt–N bonds, thus broadening the linear range of the immunosensor. Based on the above materials, a dual-electric signal outputs biosensor was constructed for detecting CA19 - 9. Under optimal conditions, the detection range of DPV and i-t is 0.001–150 U/mL, the detection limit of DPV is 0.0003 U/mL, and that of i-t is 0.00024 U/mL. In addition, the dual-electric signal outputs immunoassay excels in anti-interference, splendid reproducibility and high recovery in actual sample detection, indicating that the immunosensor is a promising approach to be applied to the detection of CA19 - 9 in clinical diagnosis.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an ultrasensitive magnetic bead-based non-competitive chemiluminescent immunoassay for 17β-estradiol in milk 牛奶中17β-雌二醇超灵敏磁珠非竞争化学发光免疫分析方法的建立

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-22 DOI: 10.1007/s00604-025-07175-0

Yi-Xian Liu, Bao-Zhu Jia, Xu-Chao Zhang, Fei Hou, Jun-Peng Liu, Wei-Yun Guo, Yuan Meng, Zhen-Lin Xu, Lin Luo

{"title":"Development of an ultrasensitive magnetic bead-based non-competitive chemiluminescent immunoassay for 17β-estradiol in milk","authors":"Yi-Xian Liu, Bao-Zhu Jia, Xu-Chao Zhang, Fei Hou, Jun-Peng Liu, Wei-Yun Guo, Yuan Meng, Zhen-Lin Xu, Lin Luo","doi":"10.1007/s00604-025-07175-0","DOIUrl":"10.1007/s00604-025-07175-0","url":null,"abstract":"<div><p>Chronic dietary intake of 17β-estradiol (E<sub>2</sub>) poses significant health risks, necessitating rapid and accurate analytical techniques for effective surveillance. This study presents a magnetic particle (MP)-assisted non-competitive chemiluminescent immunoassay (NCLIA) designed for E<sub>2</sub> quantification in milk. The NCLIA employs anti-E<sub>2</sub> monoclonal antibody (mAb)-coated MPs to capture E<sub>2</sub> target molecules, followed by the recognition of acridinium ester (AE)-conjugated anti-E<sub>2</sub>@mAb immunocomplex antibody. Magnetic separation facilitates efficient isolation of immunocomplexes before automated luminescence measurement. This NCLIA demonstrated superior performance, without the need for complex pretreatment of milk samples, significantly simplifying the analysis process. The detection limit was as low as 1.57 pg/mL, and total detection time was 8 min. Additionally, NCLIA’s results were highly consistent with those obtained by liquid chromatography-tandem mass spectrometry (LC–MS/MS), validating their reliability in analyzing real samples. Therefore, this study presents an efficient and reliable tool for the rapid screening and detection of 17β-estradiol in milk, which holds significant value for food safety monitoring.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143861387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limitations and challenges in the characterization of extracellular vesicles from stem cells and serum 干细胞和血清细胞外囊泡表征的局限性和挑战

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-21 DOI: 10.1007/s00604-025-07147-4

Sara Escudero-Cernuda, Noemi Eiro, María Fraile, Francisco J. Vizoso, Belén Fernández-Colomer, María Luisa Fernández-Sánchez

{"title":"Limitations and challenges in the characterization of extracellular vesicles from stem cells and serum","authors":"Sara Escudero-Cernuda, Noemi Eiro, María Fraile, Francisco J. Vizoso, Belén Fernández-Colomer, María Luisa Fernández-Sánchez","doi":"10.1007/s00604-025-07147-4","DOIUrl":"10.1007/s00604-025-07147-4","url":null,"abstract":"<div><p>Exosomes are a subpopulation of nanosized extracellular vesicles (EVs), formed by a lipid bilayer and naturally secreted by cells. They transport RNA, microRNAs, bioactive proteins, and lipids and play an important role in intercellular communication. Exosomes are a promising alternative cell-free therapy in regenerative medicine, immunotherapy, and drug delivery. The implementation of new exosomes treatments requires knowledge of its concentration, purity, and full characterization. However, comparing different studies is highly challenging due to the lack of validated methodologies for isolation and determination, as well as a lack of well-characterized exosomes reference standards. In this work, human uterine cervical mesenchymal stem cell (hUCESC) EVs have been isolated by ultracentrifugation and characterized, discussing the current limitations of characterization methods. First, total protein assays are heavily influenced by free-protein and lipid contaminations which confirm the need of employing several methods for vesicular protein determination. This purity variation seems heavily influenced by the vesicles origin as more complex mediums originate more matrix interferences. Size exclusion high performance liquid chromatography has been demonstrated as a new methodology for purity assessment of hUCESC-EVs and commercial EVs (adipose stem cells and human serum). The results found low purity in the commercial exosomes highlighting that protein and lipid purity must be included in the commercial EVs. Finally, the combination of this chromatography method with total protein assays proved that particle concentration could be estimated using vesicular protein concentration.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07147-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reversible and ultrafast fluorescent nanoswitch for on-site visual determination of malachite green and in vivo zebrafish imaging 用于孔雀石绿现场视觉测定和斑马鱼体内成像的可逆超快荧光纳米开关

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-04-21 DOI: 10.1007/s00604-025-07151-8

Ruxia Zhang, Qi Wang, Zhefeng Fan

{"title":"Reversible and ultrafast fluorescent nanoswitch for on-site visual determination of malachite green and in vivo zebrafish imaging","authors":"Ruxia Zhang, Qi Wang, Zhefeng Fan","doi":"10.1007/s00604-025-07151-8","DOIUrl":"10.1007/s00604-025-07151-8","url":null,"abstract":"<div><p>Malachite green (MG), one of the most toxic veterinary drug residues, is of great significance to establish a rapid, accurate, and highly selective on-site quantitative detection method for food safety. In this study, a new strategy for the detection of MG in aquatic products is developed, which is based on orange fluorescent carbon dots (O-CDs) as nanoswitches for rapid and reversible detection of MG content. The overlap between the strong absorption of MG at 615 nm and the fluorescence emission spectra of O-CDs at 564 nm provides effective support for the internal filter effect (IFE) leading to fluorescence quenching of the system. Under the attack of negative hydrogen ions in NaBH<sub>4</sub>, MG is effectively reduced to weakly absorb leucomalachite green (LMG), blocking the IFE process and leading to the recovery of system fluorescence, thereby achieving reversible detection of MG within 30 s with up to 9 cycles. In solution, the strategy demonstrates a wide linear range of 1–120 μM for MG with a detection limit of 0.37 μM. In addition, a smartphone-assisted O-CDs agar slice sensing platform has been developed, which can accurately quantify and visually determine MG. Furthermore, it is also utilized for fluorescence imaging of exogenous MG in zebrafish. In summary, the establishment of sensing strategy provides a solution for constructing qualitative and visual semi quantitative sensors on site, which has potential application value in food safety assessment.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143852610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0