Microchimica Acta最新文献_第2页

MIL-125@ZIF-8 based solid phase microextraction coating for sensitive determination of organophosphate esters in aqueous environments

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-03-28 DOI: 10.1007/s00604-025-07127-8

Xingru Hu, Jiahui Hou, Long Pang, Zhigao Feng, Junhao Liu, Haiyang Feng, Ying Kong, Chao Liu

{"title":"MIL-125@ZIF-8 based solid phase microextraction coating for sensitive determination of organophosphate esters in aqueous environments","authors":"Xingru Hu, Jiahui Hou, Long Pang, Zhigao Feng, Junhao Liu, Haiyang Feng, Ying Kong, Chao Liu","doi":"10.1007/s00604-025-07127-8","DOIUrl":"10.1007/s00604-025-07127-8","url":null,"abstract":"<div><p>A novel MOF-on-MOF heterostructure (MIL-125@ZIF-8) was synthesized and utilized as a coating material for solid-phase microextraction (SPME), which was subsequently coupled with gas chromatography (GC) for the sensitive determination of organophosphate esters (OPEs) in water samples. Compared to commercial fiber, the MIL-125@ZIF-8 coated fiber (MIL-125@ZIF-8-F) demonstrated superior extraction performance for medium-polar or non-polar compounds due to multiple interactions such as hydrophobic interaction, π-π stacking, and hydrogen bonding. The synergistic effect of MIL-125 and ZIF-8 endowed the MIL-125@ZIF-8-F with high efficiency and excellent stability under extreme conditions. Specifically, the developed MIL-125@ZIF-8-F could withstand 80 conditioning cycles without significant loss of extraction capacity. Moreover, the established method exhibited a wide linear range (10–1000 μg L<sup>−1</sup>), low detection limits (0.03–0.20 μg L<sup>−1</sup>), and excellent reproducibility under optimized condition. Finally, this proposed method provided a promising approach for the efficient enrichment of trace OPEs in actual environmental water samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A flexible magnetic DNA biosensor composed of AgNWs/hydrogel/PS/Fe3O4 for the detection of ASFV P72 protein gene fragment

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-03-28 DOI: 10.1007/s00604-025-07114-z

Mengjie Xue, Shengbo Sang, Dong Zhao, Qianqian Duan, Xing Guo

{"title":"A flexible magnetic DNA biosensor composed of AgNWs/hydrogel/PS/Fe3O4 for the detection of ASFV P72 protein gene fragment","authors":"Mengjie Xue, Shengbo Sang, Dong Zhao, Qianqian Duan, Xing Guo","doi":"10.1007/s00604-025-07114-z","DOIUrl":"10.1007/s00604-025-07114-z","url":null,"abstract":"<p>African swine fever virus (ASFV) is a highly infectious and pathogenic DNA virus with a mortality rate of nearly 100%. Here, we developed a flexible biosensor based on a AgNWs/hydrogel/PS(polystyrene)/Fe<sub>3</sub>O<sub>4</sub> composite film for the detection of ASFV P72 protein gene fragments. A large number of carboxyl groups in the hydrogel provide modification sites for capture probes (cp) simplifying the preparation process of the biosensor. Combined with the excellent magnetic properties of Fe<sub>3</sub>O<sub>4</sub>, the excellent electrical conductivity of silver nanowires (AgNWs) and the unique optical properties of two-dimensional photonic crystals (2DPC), the flexible biosensor can convert biological signals into optical signals and electrical signals, and the micro-deformation of the film can be verified by simple optical methods. In order to improve the sensitivity, we introduce Fe<sub>3</sub>O<sub>4</sub> and apply an external magnetic field to amplify the thin film micro-deformation. The concentration of P72 protein gene fragment and the relative change rate of resistance showed a good linear relationship, and the linear equation is <i>y</i> = − 0.00126x − 0.31729; the detection limit (LOD) is as low as 0.208 μM. The composite film was used to detect real serum samples, and the recovery of the composite film fluctuated in the range 91.89 to 103.19%, indicating that the composite film has practical application potential in clinical detection of ASFV. In addition, the biosensor also shows good biocompatibility, stability, and specificity.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Activity screening of Pt-CeO2 gradient films prepared by bipolar electrochemistry for electrooxidation reactions

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-03-28 DOI: 10.1007/s00604-025-07109-w

Marisa Ketkaew, Getnet Kassahun, Nashwan Hussein Ali, Patrick Garrigue, Sébastien Bonhommeau, Laurent Bouffier, Alexander Kuhn, Chularat Wattanakit, Dodzi Zigah

{"title":"Activity screening of Pt-CeO2 gradient films prepared by bipolar electrochemistry for electrooxidation reactions","authors":"Marisa Ketkaew, Getnet Kassahun, Nashwan Hussein Ali, Patrick Garrigue, Sébastien Bonhommeau, Laurent Bouffier, Alexander Kuhn, Chularat Wattanakit, Dodzi Zigah","doi":"10.1007/s00604-025-07109-w","DOIUrl":"10.1007/s00604-025-07109-w","url":null,"abstract":"<div><p>Glassy carbon electrodes were modified with a CeO<sub>2</sub> film and Pt nanoparticles (Pt-CeO<sub>2</sub>) for electrocatalysis. Interestingly, the oxidation of benzyl alcohol was significantly enhanced when Pt-CeO<sub>2</sub> films were prepared by the simultaneous electrodeposition of the two materials, indicating a significant synergistic electrocatalytic activity. Subsequently, bipolar electrochemistry was employed to prepare Pt-CeO<sub>2</sub> gradient films. Scanning electrochemical microscopy (SECM) was employed for studying local electrochemical properties at liquid/solid interfaces. SECM allowed mapping the local electrochemical performance of the Pt-CeO<sub>2</sub> gradient films for benzyl alcohol oxidation, showing that the reaction rate is proportional to the local Pt-CeO<sub>2</sub> surface coverage. Therefore, Pt-CeO<sub>2</sub> deposits with different densities along the bipolar electrode offer tunable catalytic performances for benzyl alcohol oxidation. This allows identifying in a fast and straightforward way the optimal conditions for electrocatalytic processes in a more general sense because the approach, illustrated here with one specific reaction, can be easily generalized to other catalytically active surfaces.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid photosynthesis of cellulose nanofibril-based imprinted membrane for selective colorimetric determination of isoniazid

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-03-27 DOI: 10.1007/s00604-025-07110-3

Fatima Ezzahra Rejdal, Ouarda El Hani, Abderrahman Lamaoui, Youssef Habibi, Aziz Amine

{"title":"Rapid photosynthesis of cellulose nanofibril-based imprinted membrane for selective colorimetric determination of isoniazid","authors":"Fatima Ezzahra Rejdal, Ouarda El Hani, Abderrahman Lamaoui, Youssef Habibi, Aziz Amine","doi":"10.1007/s00604-025-07110-3","DOIUrl":"10.1007/s00604-025-07110-3","url":null,"abstract":"<div><p>Molecularly imprinted membranes (MIMs) have attracted considerable interest in sensing applications. This study presents a novel rapid UV-assisted photopolymerization technique for synthesizing MIM using cellulose nanofibers (CNF) as the membrane matrix and isoniazid (INH) as the target analyte. The MIM was synthesized rapidly in 5 min, outpacing traditional methods in speed and efficiency. The integration of CNF endowed the membrane with outstanding stability in organic solvents, along with excellent mechanical flexibility and rigidity. These properties, combined with the superior tensile strength and structural integrity, make MIM an excellent candidate for high-performance sensing applications. The MIM was characterized using X-ray diffraction, thermogravimetric analysis, Fourier-transform infrared spectroscopy, mechanical testing, and scanning electron microscopy to evaluate its semicrystalline, thermal, structural, and mechanical properties. A rapid, simple, and highly sensitive colorimetric method for INH determination was developed utilizing 4-nitrobenzaldehyde and an alkaline phosphate buffer. The MIM exhibited a notable limit of detection (LOD) of 0.03 µg/mL and a limit of quantification (LOQ) of 0.1 µg/mL, with the capability to detect trace levels of INH (0.16 ng/mL) through preconcentration using a solid-phase extraction column. The method was successfully tested in spiked river water and saliva samples, yielding excellent recovery ranging from 94.21 to 100%. This MIM-based sensor provides a practical, high-performance solution for real-time, on-site INH monitoring. Its innovative design and cost-effectiveness offer substantial potential for enhancing environmental safety and public health surveillance, setting a new benchmark for field-deployable analysis technologies.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent applications and advancement of conductive hydrogels in biosensing, bioelectronics and bioengineering

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-03-27 DOI: 10.1007/s00604-025-07123-y

Shi-Yu Chen, Tao Feng, Zeng-Qiang Wu, Ning Bao

{"title":"Recent applications and advancement of conductive hydrogels in biosensing, bioelectronics and bioengineering","authors":"Shi-Yu Chen, Tao Feng, Zeng-Qiang Wu, Ning Bao","doi":"10.1007/s00604-025-07123-y","DOIUrl":"10.1007/s00604-025-07123-y","url":null,"abstract":"<div><p>Conductive hydrogels (CHs) are characterized by their distinctive three-dimensional (3D) network architecture enhanced by physically entangled or chemically cross-linked polymer chains. In recent years, these materials have garnered significant scientific interest owing to their unique combination of inherent electrical conductivity and remarkable capability to transduce external stimuli into measurable electronic signals. The immense potentials of those CHs in a number of applications catalyzed this review to summarize their specific properties and applications in biosensing, bioelectronics and bioengineering. Firstly, the preparation and unique properties of CHs were summarized, including mechanical properties, adhesion properties, self-healing capabilities, conductivity, biodegradability, and biocompatibility. We demonstrated tremendous potentials of CHs in the real world by showcasing their broad applications in biosensing (such as nerve sensing, strain sensing, glucose sensing, tumor sensing, temperature sensing, and environmental sensing), bioelectronics and bioengineering (such as treatment of cardiac and wound healing). Finally, by presenting current challenges and issues of CHs, future potential research directions were outlined for their applications in the study of biosensing, bioelectronics and bioengineering.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A core–shell AZO@ZnO nanostructure for accurate glucose detection with UV-boosted sensitivity

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-03-27 DOI: 10.1007/s00604-025-07121-0

Qi Mao, Ziyan Liu, Shuai Hu, Weixuan Jing, Fan Zhou, Bian Tian, Xiaoqin Luo, Hexiang Yang, Yijun Zhang, Wei Ren, Zhuangde Jiang

{"title":"A core–shell AZO@ZnO nanostructure for accurate glucose detection with UV-boosted sensitivity","authors":"Qi Mao, Ziyan Liu, Shuai Hu, Weixuan Jing, Fan Zhou, Bian Tian, Xiaoqin Luo, Hexiang Yang, Yijun Zhang, Wei Ren, Zhuangde Jiang","doi":"10.1007/s00604-025-07121-0","DOIUrl":"10.1007/s00604-025-07121-0","url":null,"abstract":"<p>Advances in micro-nano fabrication technology have enabled flexible electrochemical sensors to utilize micro-nanostructures and nanomaterials. Herein, a 3D AZO@ZnONRs core–shell nanostructure was synthesized using atomic layer deposition and hydrothermal techniques. The structure was employed to fabricate a high-sensitivity glucose sensor capable of precise detection of blood glucose levels and glucose content in sugary beverages. The sensor demonstrated a highly linear response (0–12.5 mM), with a sensitivity of approximately 6.49 µA·mM<sup>−1</sup>·cm<sup>−2</sup> and a detection limit of 1.561 µM. Under ultraviolet light, the sensitivity increased by 1.83-fold. In the presence of interferents such as potassium chloride, sodium chloride, lactic acid, urea, and uric acid, the sensor maintained excellent specificity. Compared to conventional nanorods, this 3D core–shell material preserved the advantages of a high specific surface area while demonstrating enhanced electron transfer capabilities and photosensitivity, enabling reliable detection of glucose at extremely low concentrations. This study systematically analyzed the characteristics of the core–shell nanomaterial and its photocatalytic mechanisms, advancing photocatalytic electrochemical sensing technology.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ZnO nanoflower-mediated paper-based electrochemical biosensor for perfect classification of cardiac biomarkers with physics-informed machine learning

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-03-26 DOI: 10.1007/s00604-025-07102-3

Partha Pratim Goswami, Aditya Vikram Singh, Shiv Govind Singh

{"title":"ZnO nanoflower-mediated paper-based electrochemical biosensor for perfect classification of cardiac biomarkers with physics-informed machine learning","authors":"Partha Pratim Goswami, Aditya Vikram Singh, Shiv Govind Singh","doi":"10.1007/s00604-025-07102-3","DOIUrl":"10.1007/s00604-025-07102-3","url":null,"abstract":"<p>The widespread exposure of acute myocardial infarction globally demands an ultrasensitive, rapid, and cost-effective biosensor for troponin-I and T in a dynamic concentration range. Traditionally, the saturation of sensor response limits accurate prediction at high analyte concentrations, although this is seldom discussed in the literature. To address this research gap, we thematically report physics-informed analytical treatments with machine learning (PIML) on a paper-based electrochemical biosensor, taking advantage of low cost, flexibility, low sample volume, and ease of deployment. Owing to the well-known biosensing performances, ZnO nanoflowers, synthesized in-house with a hydrothermal procedure, are utilized for transduction purposes with three voltametric techniques: CV, DPV, and SWV. The exceptional surface coverage and high IEP of ZnO have contributed towards the realization of high sensitivity, and the monoclonal antibody-based bioreceptors ensured the enormous selectivity of the platform. Nevertheless, the traditional calibration approach for CV considers the peak current as the sensor parameter, which gets flattened at higher concentrations, thereby limiting reliability. Therefore, this issue is addressed by strategic analytical development by extracting the charge associated with a CV scan and employing this physics-informed feature in the machine learning (ML) model. Combining features generated from different electrochemical techniques in the ML model enhances data diversity by including comprehensive information. This unique approach towards data analysis led to achieving 100% accuracy and AUC scores for identifying cardiac troponin-I and T multiclass concentrations. We strongly believe that the proposed methodologies have a substantial potential for translation to any other related sensor applications.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143698469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An effective strategy based on electrostatic interaction for the simultaneous sequential purification and isolation of exosomes

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-03-26 DOI: 10.1007/s00604-025-07125-w

Haihong Chen, Miaoxia Ma, Lingyi Zhang, Zhengduo Wang, Haofan Sun, Chenyu Liu, Lixin Zhang, Weibing Zhang

{"title":"An effective strategy based on electrostatic interaction for the simultaneous sequential purification and isolation of exosomes","authors":"Haihong Chen, Miaoxia Ma, Lingyi Zhang, Zhengduo Wang, Haofan Sun, Chenyu Liu, Lixin Zhang, Weibing Zhang","doi":"10.1007/s00604-025-07125-w","DOIUrl":"10.1007/s00604-025-07125-w","url":null,"abstract":"<div><p> A material-based strategy was developed to achieve the simultaneous purification and isolation of exosomes from serum and urine. Based on the combination of electrostatic interaction and hydrophobic interaction, polyacrylic acid (PAA)–coated anionic nanoparticles (Fe<sub>3</sub>O<sub>4</sub>@PAA) were used to remove positively charged contaminant proteins and neutral proteins, and polyethyleneimine (PEI)-functionalized cationic nanoparticles (Fe<sub>3</sub>O<sub>4</sub>@PEI) were applied to remove negatively charged contaminant proteins as well as capture and mild release of negatively charged exosome. By employing this strategy (denoted as PAA-PEI), a high recovery (> 95%) of serum exosomes was achieved with a high removal efficiency of protein contaminants (87%). The strategy was further applied to purify and isolate urinary exosomes, followed by downstream proteomics analysis. Compared with the standard isolation method of ultracentrifugation (UC), the PAA-PEI strategy shows high contaminant protein removal efficiency (98.6%) and obtains a higher concentration of exosomes. Using the PAA-PEI strategy, more urinary exosomal proteins (124) than UC (92) were identified. These results indicate that the PAA-PEI strategy is not only excellent in exosome capture but also effectively mitigates the interference of protein contamination. Given that the PAA-PEI strategy demonstrates a high protein contaminant removal efficiency and requires less cost and time (1 h) than UC (3 h), it would be a promising candidate method for efficiently purifying and isolating exosomes from complex biological samples for early discovery and diagnosis diseases. Furthermore, this study provides a new direction for emphasizing the issue of protein interference in the development of biological sample isolation methods.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143698470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ratiometric fluorescent detection of Hg2+ using dual-emissive carbon dots in an immiscible system

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-03-26 DOI: 10.1007/s00604-025-07097-x

Fei Shen, Xiangzhi Meng, Wenxi Zhang, Jing Sun, Juan Hou

{"title":"Ratiometric fluorescent detection of Hg2+ using dual-emissive carbon dots in an immiscible system","authors":"Fei Shen, Xiangzhi Meng, Wenxi Zhang, Jing Sun, Juan Hou","doi":"10.1007/s00604-025-07097-x","DOIUrl":"10.1007/s00604-025-07097-x","url":null,"abstract":"<div><p> A facile one-pot hydrothermal method was established to simultaneously prepare blue and yellow emissive carbon dots (BCDs and YCDs) in an immiscible system. This method eliminated the need for complex purification steps. The resulting BCDs and YCDs exhibited uniform sizes and excellent fluorescence properties. A ratiometric fluorescent probe based on B/YCDs was designed for the rapid and selective detection of Hg<sup>2+</sup> with a detection limit of 15 nM. The probe was capable of reliable detection in real water samples with recoveries of 92.42 ~ 110.02%. To further enhance practicality, we incorporated fluorescent papers and hydrogels for visual Hg<sup>2+</sup> detection, making it ideal for on-site applications. Additionally, a smartphone-based sensing platform, integrating RGB value analysis, was also developed for accurate Hg<sup>2+</sup> detection. This work represents a significant advancement in the field by providing a user-friendly and cost-effective approach for sensitive on-site detection of hazardous mercury pollution.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143698645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fluorescence ‘turn-on’ sensing of glial fibrillary acidic protein using graphene oxide-quenched copper nanoclusters

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-03-26 DOI: 10.1007/s00604-025-07103-2

Geneva Indongo, Merin K. Abraham, Greeshma Rajeevan, Arathy B. Kala, Dheyaa Mohammed Dhahir, Sony George

{"title":"Fluorescence ‘turn-on’ sensing of glial fibrillary acidic protein using graphene oxide-quenched copper nanoclusters","authors":"Geneva Indongo, Merin K. Abraham, Greeshma Rajeevan, Arathy B. Kala, Dheyaa Mohammed Dhahir, Sony George","doi":"10.1007/s00604-025-07103-2","DOIUrl":"10.1007/s00604-025-07103-2","url":null,"abstract":"<div><p>This study introduces a fluorescence based sensing platform made to detect glial fibrillary acidic protein (GFAP), a critical biomarker associated with glioblastoma and other astrocytic malignancies. Leveraging the unique optical properties of copper nanoclusters (CuNCs) functionalized with GFAP antibodies (GFAP Ab), the platform incorporates graphene oxide (GO) as a fluorescence quencher to create a highly sensitive turn on sensor responsive to GFAP antigens. The detection mechanism relies on Förster resonance energy transfer (FRET), wherein the binding of GFAP antigens disrupts the GFAP Ab@CuNCs-GO interaction, effectively restoring fluorescence. The CuNCs stabilized with l-cysteine to enhance biocompatibility and stability, exhibited strong green fluorescence with a quantum yield of 1.0%. Graphene oxide efficiently quenched the fluorescence of GFAP Ab@CuNCs therefore enhancing the platform’s sensitivity. The sensor displayed a linear fluorescence response across a GFAP concentration range 0–46 ng/mL, with a detection limit of 32 pg/mL, demonstrating its capability to detect GFAP at clinically relevant levels. Validation of the sensor in biological fluids, including saliva, serum and urine, confirmed its applicability for minimally invasive diagnostics. Situated at the intersection of biosensing and clinical relevance, this study aims to address the need for cost effective and accessible diagnostic and screening tools for glioblastoma.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0