Microchimica Acta最新文献

{"title":"Highly sensitive SERS-based lateral flow immunoassay of fipronil using bimetallic Au@Ag@Ag nanorods","authors":"Kseniya V. Serebrennikova,&nbsp;Nadezhda S. Komova,&nbsp;Lyubov V. Barshevskaya,&nbsp;Anatoly V. Zherdev,&nbsp;Boris B. Dzantiev","doi":"10.1007/s00604-024-06811-5","DOIUrl":"10.1007/s00604-024-06811-5","url":null,"abstract":"<div><p>A bimetallic core–shell-shell nanorods structure with gap-embedded Raman reporter 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) was developed and applied as a SERS-active nanotag in surface-enhanced Raman scattering lateral flow immunoassay (SERS-LFIA) of the insecticide fipronil. Due to the strong SERS signal of the Au^DTNB@Ag^DTNB@AgNRods, fipronil is detected with extremely low detection limit of 4.6 pg/mL. To the best of our knowledge, the proposed technique is the first SERS-LFIA of fipronil, proven to be effective in the selective determination of the target analyte and capable of detecting fipronil in a matrix of food samples (cucumber and apple juice) with recoveries of 97.0–117.0%. Moreover, the applied functionalization of the SERS nanotag with anti-species antibodies has provided a versatile immunoprobe that could improve performance of different LFIAs.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis and utilization of mixed-metal (Zn/Cd) metal–organic frameworks for ultra-trace determination of diazinon and ethion via ion mobility spectrometry","authors":"Laleh Khorshidi,&nbsp;Behzad Aibaghi","doi":"10.1007/s00604-024-06830-2","DOIUrl":"10.1007/s00604-024-06830-2","url":null,"abstract":"<div><p>A novel mixed-metal (Zn/Cd) metal–organic framework-based dispersive solid-phase microextraction (MM-MOFs-DSPME) method was developed for the efficient extraction of organophosphorus pesticides, diazinon and ethion, from various environmental matrices. The detection of the extracted analytes was performed using ion mobility spectrometry. The synthesized mixed-metal (Zn/Cd) MOF was characterized using a comprehensive array of analytical techniques: Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), energy-dispersive X-ray spectroscopy (EDX), Brunauer–Emmett–Teller (BET) surface area analysis, X-ray photoelectron spectroscopy (XPS), and zeta potential measurement. This comprehensive characterization provided an in-depth understanding of the material’s structural and functional properties. Key parameters influencing the efficiency of the dispersive solid-phase microextraction (DSPME) method were optimized, including pH, type of desorption solvent, buffer type and volume, sorbent amount, and adsorption and desorption time. Under these optimal conditions, the linear dynamic ranges obtained were 0.5 to 300 ng mL⁻¹ for diazinon and 1.0 to 300 ng mL⁻¹ for ethion, with limits of detection of 0.15 ng mL⁻¹ and 0.29 ng mL⁻¹, respectively. Preconcentration factors of 74% and 78%, with extraction recoveries of 98.4–104.2% and 96.6–103.4%, were achieved for diazinon and ethion, respectively. The relative standard deviations, calculated based on ten replicate measurements, yielded values of 3.8% for diazinon and 3.9% for ethion at a concentration of 10 ng mL⁻¹, and 2.2% for diazinon, and 1.6% for ethion at a concentration of 150 ng mL⁻¹, respectively. The developed method was successfully applied to the quantification of the target pesticides in soil, water, and apple samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142646556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ammonia and temperature sensing applications using fluorometric and colorimetric microparticles and polymeric films doped with BODIPY-emitters","authors":"Beatriz S. Cugnasca,&nbsp;Frederico Duarte,&nbsp;Hugo M. Santos,&nbsp;José Luis Capelo-Martínez,&nbsp;Emilia Bértolo,&nbsp;Alcindo A. Dos Santos,&nbsp;Carlos Lodeiro","doi":"10.1007/s00604-024-06814-2","DOIUrl":"10.1007/s00604-024-06814-2","url":null,"abstract":"<div><p>Four functionalized BODIPY derivatives (BDP1 to BDP4) were synthesized and their optical properties investigated both in solution and when incorporated into a solid matrix. Recognizing the versatility of BODIPY derivatives and the increasing interest in developing new luminescent organic dyes embedded in polymers, the BODIPY derivatives were dispersed into two types of polymeric matrices: Poly(methyl methacrylate) (PMMA) and Thermoplastic Polyurethane (TPU), both as films and microparticles. This resulted in eight new BODIPY-doped polymer films and eight types of BODIPY-doped polymeric microparticles for use in aqueous solutions. The integration of the BODIPY dyes into the polymeric matrices combines the unique properties of the polymer films, such as porosity, flexibility, and elasticity, with the excellent photophysical characteristics of the BODIPYs. Importantly, the dispersion minimized issues such as aggregation-caused quenching commonly observed in solid-state luminescent materials. The thermometric responses of all polymer films were evaluated by studying their solid-state emission spectra in the 25–200 °C temperature range. The reversibility of these temperature-induced changes was also assessed, revealing excellent recovery of luminescence. These promising results suggest these materials could have applications as fluorescent thermometric sensors. Furthermore, we explored the potential of the brominated (BDP3) and chalcogenated (BDP4) BODIPY derivatives as ammonia sensors. The two derivatives produced yellow fluorescent products upon interaction with the analyte. Kinetic studies using solid-state emission spectra of BDP4@TPU and BDP4@PMMA showed significant differences in reaction rates (minutes for BDP4@TPU and hours in the case of BDP4@PMMA) attributable to the higher permeability of TPU when compared with PMMA. Detection and quantification of ammonia concentration were conducted by means of simple photographic analysis, measuring the “R” (red) and “G” (green) components of RGB color parameters. The results from the photographic method correlated well with the results from fluorimetric spectroscopy studies. The photographic analysis is straightforward, portable, and does not require expensive equipment. Finally, we successfully applied polymeric microparticles doped with BODIPYs to detect ammonia in water, demonstrating their effectiveness without the need for organic solvents. This highlights their potential for environmental monitoring and other applications requiring sensitive and selective detection methods.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142643630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon dot-graphene oxide-based luminescent nanosensor for creatinine detection in human urine","authors":"Poornima Bhatt,&nbsp;Deepak Kukkar,&nbsp;Ashok Kumar Yadav","doi":"10.1007/s00604-024-06838-8","DOIUrl":"10.1007/s00604-024-06838-8","url":null,"abstract":"<div><p>A fluorescence (FL)-based nanosensor has been devised for creatinine (CR) detection in human urine specimens. The proposed nanosensor utilized a nanocomposite (NC) of carbon dots (CDs) and graphene oxide (GO). The formation of CDs/GO NC reduced the CD FL emission (<i>λ</i>_excitation = 390 nm, <i>λ</i>_emission = 461 nm) by ~ 75%. With the introduction of CR to the NC, the CD emission intensity was reinstated by approximately 70%. The linear detection range for CR was 10⁻⁵ to 0.1 mg dL⁻¹ (<i>R</i>² = 0.998), with a limit of detection of 4.3 × 10⁻² mg dL⁻¹. Additionally, CDs/GO NC exhibited outstanding consistency and specificity in recognizing CR within urine specimens from both healthy individuals and patients suffering from chronic kidney disease (CKD). The Bland–Altman assessment (utilizing 25 human urine specimens) displayed remarkable consensus (<i>R</i>² = 0.995) among the FL approach and the benchmark Jaffe technique. This observation indicates the hands-on usefulness of the nanosensor for identifying CR in biological specimens. </p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142638298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel miniaturized potentiometric electrode based on carbon nanotubes and molecularly imprinted polymer for the determination of lidocaine","authors":"Saad S. M. Hassan,&nbsp;Mahmoud Abdelwahab Fathy","doi":"10.1007/s00604-024-06802-6","DOIUrl":"10.1007/s00604-024-06802-6","url":null,"abstract":"<div><p>A novel miniaturized, solid-contact potentiometric screen-printed electrode was developed for highly sensitive and selective determination of lidocaine anesthetic. The electrode integrated single-walled carbon nanotubes as a solid-contact material and a molecularly imprinted polymer as a recognition sensory material. The performance characteristics of the electrode were evaluated and optimized to display a Nernstian slope of 58.92 ± 0.98 mV/decade over a linear concentration range of 4.53 × 10⁻⁷ to 6.18 × 10⁻³ mol/l within &lt; 6 s. The detection limit was 7.75 × 10⁻⁸ mol/l (18.16 ng/ml) of lidocaine. The use of the molecularly imprinted polymer significantly enhanced the selectivity of the electrode, and carbon nanotubes increased the sensitivity, accuracy, and potential stability. The electrode was successfully used for determining lidocaine in pharmaceutical preparations and human urine. The results favorably compared with data obtained by liquid chromatography–tandem mass spectrometry.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06802-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of CuFe2O4 microspheres/carbon sheets composite materials as a sensitive electrochemical sensor for determination of bisphenol A","authors":"Ying Wang,&nbsp;Xinming Wang,&nbsp;Mingyue Chu,&nbsp;Jianjiao Xin,&nbsp;Zhongxin Jin,&nbsp;Huiyuan Ma,&nbsp;Kevin P. O’Halloran,&nbsp;Yingji Wang,&nbsp;Haijun Pang,&nbsp;Guixin Yang","doi":"10.1007/s00604-024-06806-2","DOIUrl":"10.1007/s00604-024-06806-2","url":null,"abstract":"<div><p>A composite material based on CuFe-ZIF-derived CuFe₂O₄ nano-microspheres grown <i>in situ</i> and well-ordered on carbon sheets (CS) was prepared and applied for highly effective determination of bisphenol A (BPA). The composite material possessed inherently high redox activity due to the presence of both Cu and Fe ions with various oxidation states (Cu²⁺/Cu⁺ and Fe³⁺/Fe²⁺), high specific surface area, uniform distribution of Cu and Fe ions, and a robust framework imparted by its precursor CuFe-ZIF. This led to increased active sites for electrochemical reactions, improved electron transfer efficiency, and structural integrity during electrochemical cycling. Furthermore, combining CS with CuFe₂O₄ not only provided a large surface area to support well-ordered CuFe₂O₄ nano-microspheres without aggregation, but also enhanced the conductivity and mechanical stability of the CuFe₂O₄/CS composite. This results in synergistic effects that enhanced the overall performance of the composite material. In addition, both copper and iron are relatively non-toxic and abundant, making CuFe₂O₄/CS safe and cost-effective for large-scale applications. Consequently, the CuFe₂O₄/CS-modified electrode shows highly efficient electrochemical sensing properties with a wider detection range of 0.009-168 µM and lower detection limit of 0.0027 µM (S/<i>N</i> = 3) compared with most reported BPA sensors. It also has an optimized current at pH 7 which is convenient for real world applications. This CuFe₂O₄/CS modified electrode as a highly sensitive electrochemical platform can be applied to monitor BPA concentrations in bottled water with good recovery (97.2-102.2%).</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conducting polymer functionalized Cu-metal organic framework–based electrochemical immunosensor for rapid and sensitive quantitation of Escherichia coli O157:H7","authors":"Xiaoge Duan,&nbsp;Xinrui Shi,&nbsp;Zhaoyuan He,&nbsp;Hongcai Chen,&nbsp;Zhuanzhuan Shi,&nbsp;Zhi Zhao,&nbsp;Hailan Chen,&nbsp;Meiling Yu,&nbsp;Chunxian Guo","doi":"10.1007/s00604-024-06807-1","DOIUrl":"10.1007/s00604-024-06807-1","url":null,"abstract":"<div><p><i>Escherichia</i> <i>coli</i> (<i>E. coli</i>) O157:H7 is an important food-borne pathogen that can cause hemorrhagic diarrhea and enteritis in humans and animals. Realizing the rapid quantitation of <i>E. coli</i> O157:H7 is of great significance for the guarantee of food safety and disease control. In this study, an electrochemical immunosensing technique based on a functionalized composite of Cu-metal organic framework (Cu-MOF) and poly (3,4-ethylenedioxythiophene)-poly (styrenesulfonate) (PEDOT:PSS) is developed, achieving rapid and sensitive quantitation of <i>E. coli</i> O157:H7 in food and clinical feces samples. The organic functionalization of Cu-MOF significantly improves the interface conductivity to facilitate electron transfer and provides the sulfonic groups (–SO₃H) to conjugate bio-recognizing elements for target determination. The immunosensor delivers a linear detection range of 3 × 10² ~ 3 × 10⁸ cfu/mL, a low limit of detection (LOD) of 7.4 cfu/mL, and a short analysis time of 40 min. In addition, it does not show any cross-reactivity with other common pathogens and exhibits high repeatability with relative standard deviations (RSDs) all lower than 2.09%, providing a promising approach for warranting  food safety and control of <i>E. coli</i> O157:H7 disease.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly sensitive detection of MMP-2 using an electrochemiluminescent biosensor enhanced by ladder-branch hybridization chain reaction","authors":"Yuanxun Gong,&nbsp;Jiayi Zhang,&nbsp;Yunzhan Xi,&nbsp;Dingxie Jian,&nbsp;Chenyi Zhuo,&nbsp;Qianli Tang,&nbsp;Kai Zhang,&nbsp;Xianjiu Liao","doi":"10.1007/s00604-024-06761-y","DOIUrl":"10.1007/s00604-024-06761-y","url":null,"abstract":"<div><p> An advanced electrochemiluminescence (ECL) biosensor was developed that integrates T7 RNA polymerase amplification, ladder-branch hybridization chain reaction (HCR), and the precise targeting capabilities of CRISPR/Cas13a technology. The novelty of this research lies in the unique combination of these three cutting-edge technologies, which has not been previously utilized together in biosensing platforms, enabling highly sensitive and specific detection of biomolecules with exceptional precision. This innovative biosensor addresses the critical need for sensitive and specific detection of matrix metalloproteinase-2 (MMP-2), a key biomarker in cancer diagnostics. Through meticulous optimization of amplification and reaction conditions, the biosensor demonstrated remarkable sensitivity and specificity, achieving a detection limit as low as 6.34 aM, surpassing existing methodologies. The biosensor also exhibited excellent stability and reproducibility across multiple scans and maintained consistent functionality over an extended period, highlighting its reliability for practical applications. The effectiveness of the biosensor was validated using real samples, demonstrating its capability to accurately quantify MMP-2 in complex biological matrices with high recovery and minimal interference. The integration of isothermal amplification and CRISPR/Cas13a within the ECL biosensor platform represents a significant advancement in molecular diagnostics, offering a powerful tool for real-time monitoring of MMP-2. This combination of technologies sets the platform apart from traditional methods, marking a significant step forward in biosensor innovation. This biosensor holds substantial promise for revolutionizing cancer diagnostics and facilitating personalized treatment strategies, ultimately aiming to improve patient outcomes in cancer care. Future research may explore further enhancements and applications of this biosensor in various clinical settings.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142611846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic sensor using pH gradient with hybrid magnetoliposomes containing laccase immobilized nanocrystals","authors":"Vanesa Román-Pizarro,&nbsp;Ángela Écija-Arenas,&nbsp;Cornelia A. Hermann,&nbsp;Thomas Hirsch,&nbsp;Juan Manuel Fernández-Romero","doi":"10.1007/s00604-024-06829-9","DOIUrl":"10.1007/s00604-024-06829-9","url":null,"abstract":"<div><p>A method using pH gradient to modify the phospholipid dissociation has been developed using hybrid magnetoliposomes with encapsulated enzyme laccase, which has been immobilized on hydrophilic magnetic nanocrystals (PAA-MNCs) retained in the reaction/detection zone of a microfluidic system. The hybrid magnetoliposomes act as micro containers, providing the safe transfer of the enzyme to the reaction/detection site, where it is retained, and preconcentration of the enzyme in this place occurs, which improves the system’s sensitivity. The use of pH change involves the lack of external molecules to release the encapsulated materials. The laccase-mediated redox oxidation of the fluorescent substrate 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) to form its non-fluorescent oxide form has been used as the indicator reaction. The method was applied to determine potential laccase inhibitors in beverages and fruits. The study of the inhibitory effect has focused on compounds that follow different mechanisms: cysteine, malic acid, and fumaric acid. The dynamic ranges of the calibration graphs of these compounds were between 0.05–100, 0.02–100, and 0.1–100 μmol L⁻¹, respectively. The detection limits have been established between 6 and 30 nmol L⁻¹, and the precision expressed as the percent of relative standard deviation (RSD%) was between 2.5 and 5.1%. The overall microfluidic system showed a sampling frequency of 8 h⁻¹. The method has been applied to determine fumaric acid as a laccase inhibitor in several juice samples (apple, pear, and grape), with recovery values between 85 and 101%. The results were comparable to those obtained using a LC reference method.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Designing a molecularly imprinted polymer-based electrochemical sensor for the sensitive and selective detection of the antimalarial chloroquine phosphate","authors":"Ensar Piskin,&nbsp;Ahmet Cetinkaya,&nbsp;Mehmet Altay Unal,&nbsp;Briza Pérez-López,&nbsp;Udara Bimendra Gunatilake,&nbsp;Saad Sene,&nbsp;Yannick Guari,&nbsp;Joulia Larionova,&nbsp;Eva Baldrich,&nbsp;Sibel A. Ozkan","doi":"10.1007/s00604-024-06820-4","DOIUrl":"10.1007/s00604-024-06820-4","url":null,"abstract":"<div><p>For the first time an electrochemical sensor based on nanomaterial-supported molecularly imprinted polymers (MIPs) is applied to the sensitive and specific determination of chloroquine phosphate (CHL). The sensor was produced using an electropolymerization (EP) approach, and it was formed on a glassy carbon electrode (GCE) using CHL as a template and 2-acrylamido-2-methyl-1-propane sulfonic acid (AMPS) and aniline (ANI) as functional monomers. Incorporating Prussian blue polyethyleneglycol-amine nanoparticles (PB@PEG-NH₂) in the MIP-based electrochemical sensor increased the active surface area and porosity. The developed CHL/AMPS-PANI/PB@PEG-NH₂/MIP-GCE sensor was characterized morphologically and electrochemically using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), and scanning electron microscopy (SEM). The indirect measurement of CHL was accomplished in 5.0 mmol L⁻¹ [Fe(CN)₆]^−3/−4 solution using differential pulse voltammetry, displaying linear response between 1.75 × 10⁻¹² and 2.50 × 10⁻¹³ M, and limits of detection (LOD) and quantitation (LOQ) of 6.68 × 10⁻¹⁴ M and 2.23 × 10⁻¹³ M, respectively, in standard solutions. CHL recoveries in spiked serum and tablet form ranged from 99.13 to 101.51%, while the relative standard deviations (RSD%) were below 2.41% in both types of samples. In addition, the sensor’s excellent selectivity was successfully demonstrated in the presence of components with a chemical structure similar to CHL.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142600518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}