{"title":"基于聚（5-氨基吲哚）的肿瘤生物标志物戊烷素-3检测电化学免疫传感器","authors":"Katesara Phasuksom, Natthaporn Thongwattana, Nuttha Ariyasajjamongkol, Napa Parinyanitikul, Anuvat Sirivat","doi":"10.1007/s00604-025-07174-1","DOIUrl":null,"url":null,"abstract":"<div><p>Pentraxin-3 (PTX-3) plays a critical role in regulating inflammation, immune response, angiogenesis, and tissue remodeling. As a serum biomarker, it holds a potential in cancer diagnostics and monitoring. Elevated expression of PTX-3 has been found in various cancers, namely lung, prostate, gastric, breast, and hepatic cancers. Here, a PTX-3 immunosensor was fabricated by utilizing poly(5-aminoindole) (P5AIn) to increase the current response and the electroactive surface area (ESA). (3-Aminopropyl)triethoxysilane (APTES) was used as a linker and as an electron transfer enhancer. The developed PTX-3 immunosensor (BSA/anti-PTX-3/APTES/P5AIn/SPCE) shows excellent performance for PTX-3 detection in a wide concentration range from 0.0001 to 2000 ng/mL with the LOD of 0.012 pg/mL. Furthermore, the sensor possessed good reproducibility (%RSD = 0.78), a long shelf-life of at least 4 weeks (% RSD = 0.89), and high specificity to PTX-3. The fabricated PTX-3 immunosensor was demonstrated in the measurement of PTX-3 concentrations in real blood serums from gastric patients. It has been shown as a potentially promising portable sensor for cancer diagnostics and monitoring for cancer patients in remote areas with a low cost.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Electrochemical immunosensor based on poly(5-aminoindole) for cancer biomarker pentraxin-3 detection\",\"authors\":\"Katesara Phasuksom, Natthaporn Thongwattana, Nuttha Ariyasajjamongkol, Napa Parinyanitikul, Anuvat Sirivat\",\"doi\":\"10.1007/s00604-025-07174-1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Pentraxin-3 (PTX-3) plays a critical role in regulating inflammation, immune response, angiogenesis, and tissue remodeling. As a serum biomarker, it holds a potential in cancer diagnostics and monitoring. Elevated expression of PTX-3 has been found in various cancers, namely lung, prostate, gastric, breast, and hepatic cancers. Here, a PTX-3 immunosensor was fabricated by utilizing poly(5-aminoindole) (P5AIn) to increase the current response and the electroactive surface area (ESA). (3-Aminopropyl)triethoxysilane (APTES) was used as a linker and as an electron transfer enhancer. The developed PTX-3 immunosensor (BSA/anti-PTX-3/APTES/P5AIn/SPCE) shows excellent performance for PTX-3 detection in a wide concentration range from 0.0001 to 2000 ng/mL with the LOD of 0.012 pg/mL. Furthermore, the sensor possessed good reproducibility (%RSD = 0.78), a long shelf-life of at least 4 weeks (% RSD = 0.89), and high specificity to PTX-3. The fabricated PTX-3 immunosensor was demonstrated in the measurement of PTX-3 concentrations in real blood serums from gastric patients. It has been shown as a potentially promising portable sensor for cancer diagnostics and monitoring for cancer patients in remote areas with a low cost.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 5\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-04-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-025-07174-1\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07174-1","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Electrochemical immunosensor based on poly(5-aminoindole) for cancer biomarker pentraxin-3 detection
Pentraxin-3 (PTX-3) plays a critical role in regulating inflammation, immune response, angiogenesis, and tissue remodeling. As a serum biomarker, it holds a potential in cancer diagnostics and monitoring. Elevated expression of PTX-3 has been found in various cancers, namely lung, prostate, gastric, breast, and hepatic cancers. Here, a PTX-3 immunosensor was fabricated by utilizing poly(5-aminoindole) (P5AIn) to increase the current response and the electroactive surface area (ESA). (3-Aminopropyl)triethoxysilane (APTES) was used as a linker and as an electron transfer enhancer. The developed PTX-3 immunosensor (BSA/anti-PTX-3/APTES/P5AIn/SPCE) shows excellent performance for PTX-3 detection in a wide concentration range from 0.0001 to 2000 ng/mL with the LOD of 0.012 pg/mL. Furthermore, the sensor possessed good reproducibility (%RSD = 0.78), a long shelf-life of at least 4 weeks (% RSD = 0.89), and high specificity to PTX-3. The fabricated PTX-3 immunosensor was demonstrated in the measurement of PTX-3 concentrations in real blood serums from gastric patients. It has been shown as a potentially promising portable sensor for cancer diagnostics and monitoring for cancer patients in remote areas with a low cost.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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