A dual-channel lipid droplet-targeting probe for the detection of polarity and HOCl in models of NAFLD, inflammation, and cancer

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-04-25 DOI:10.1007/s00604-025-07171-4

Tingting Yang, Shiyu Yin, Huina Liu, Shunyi Wang, Chengqi Wang, Yuxin Chen, Xiaoya Liu, Fangke Zhu, Jiaxuan Guo, Lajiatai, Dongzhi Suonanmu, Qingjia Ren, Shenghua Liao, Caolong Li

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引用次数: 0

Abstract

Abnormal lipid droplet accumulation and metabolism can precipitate various diseases. Understanding the dynamics of intracellular lipid droplets, particularly hypochlorite acid (HOCl) and polarity, is crucial for elucidating the onset and progression of these diseases. In this study, we synthesized a near-infrared fluorescent probe, NSSP, featuring a D-π-A structure, which facilitates the independent dual-response detection of HOCl and polarity. NSSP not only reveals the polarity and HOCl fluctuations in inflammatory cells and inflammation in live mice but also differentiates between normal and fatty liver tissues through two distinct channels. Furthermore, the probe has proven effective in identifying neoplasms within live murine models at both the cellular and systemic levels, thereby affirming its utility as an instrumental resource for the precocious detection of carcinomas. This offers a hopeful avenue for the diagnostic scrutiny of pathologies.

Graphical Abstract

查看原文本刊更多论文

一种双通道脂滴靶向探针，用于检测NAFLD、炎症和癌症模型中的极性和HOCl

脂滴积聚和代谢异常可诱发多种疾病。了解细胞内脂滴的动力学，特别是次氯酸（HOCl）和极性，对于阐明这些疾病的发生和进展至关重要。在本研究中，我们合成了一种近红外荧光探针NSSP，该探针具有D-π-A结构，可以实现HOCl和极性的独立双响应检测。NSSP不仅揭示了活鼠炎症细胞和炎症的极性和HOCl波动，而且通过两个不同的通道区分正常和脂肪肝组织。此外，该探针已被证明在细胞和系统水平上有效地识别活体小鼠模型中的肿瘤，从而肯定了其作为过早检测癌症的工具资源的实用性。这为病理的诊断审查提供了一个有希望的途径。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.