Microchimica Acta最新文献_第3页

Correction: Highly sensitive detection of MMP-2 using an electrochemiluminescent biosensor enhanced by ladder-branch hybridization chain reaction 校正：采用阶梯-分支杂交链式反应增强的电化学发光生物传感器进行高灵敏度的MMP-2检测

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-17 DOI: 10.1007/s00604-024-06919-8

Yuanxun Gong, Jiayi Zhang, Yunzhan Xie, Dingxi Jian, Chenyi Zhuo, Qianli Tang, Kai Zhang, Xianjiu Liao

引用次数: 0

Lab-on-paper for molecular testing with USB-powered isothermal amplification and fluidic control 实验室纸上分子测试与usb供电等温放大和流体控制

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-17 DOI: 10.1007/s00604-024-06889-x

Hyun Pyo Kim, Ji-Ho Park, Min-Gon Kim, Youngung Seok

{"title":"Lab-on-paper for molecular testing with USB-powered isothermal amplification and fluidic control","authors":"Hyun Pyo Kim, Ji-Ho Park, Min-Gon Kim, Youngung Seok","doi":"10.1007/s00604-024-06889-x","DOIUrl":"10.1007/s00604-024-06889-x","url":null,"abstract":"<div><p>The global healthcare market increasingly demands affordable molecular diagnostics for field testing. To address this need, we introduce a lab-on-paper (LOP) platform that integrates isothermal amplification with a specially designed paper strip for molecular testing through an automated microfluidics process. The LOP system is engineered for rapid, cost-effective, and highly sensitive detection, using USB-powered thermal management and a wax valve mechanism. This innovative platform provides an accessible solution for the rapid and accurate detection of various microorganisms, proving particularly advantageous for point-of-care testing in resource-limited environments. Experiments conducted in this study demonstrated the efficacy of the LOP platform in the colorimetric detection of foodborne pathogens. It reliably detected <i>Vibrio vulnificus</i> at concentrations as low as 120 CFU/mL and <i>Salmonella Typhimurium</i> at 60 CFU/mL, with results observable to the naked eye. The entire process, encompassing amplification and detection, was completed within 30 min, underscoring the system’s rapid diagnostic capability. Furthermore, with an assay cost of 5.2 USD per test, the platform offers a highly cost-effective solution for molecular diagnostics, particularly in resource-limited settings. The LOP platform’s portability, ease of use, and affordability make it a promising alternative for various diagnostic applications, including infectious disease monitoring and ensuring food safety.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An analytical review of the therapeutic application of recent trends in MIL-based delivery systems in cancer therapy 基于mil的给药系统在癌症治疗中的最新应用趋势分析综述

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-16 DOI: 10.1007/s00604-024-06944-7

Mohammad Beiranvand, Gholamreza Dehghan

{"title":"An analytical review of the therapeutic application of recent trends in MIL-based delivery systems in cancer therapy","authors":"Mohammad Beiranvand, Gholamreza Dehghan","doi":"10.1007/s00604-024-06944-7","DOIUrl":"10.1007/s00604-024-06944-7","url":null,"abstract":"<div><p>MILs (Materials Institute Lavoisier), as nanocarriers based on metal–organic frameworks (MOFs), are one of the most advanced drug delivery vehicles that are now a major part of cancer treatment research. This review article highlights the key features and components of MIL nanocarriers for the development and improvement of these nanocarriers for drug delivery. Surface coatings are one of the key components of MIL nanocarriers, which play the role of stabilizing the nanocarrier, pH-dependent drug release, increasing the half-life of the drug, and targeting the carrier. MIL nanocarriers have been synthesized mainly by thermal and hydrothermal methods due to their single-step nature and the ability to produce individual crystals with tunable sizes. According to the data available in the literature, MIL-53 and MIL-101 are the best MILs for drug delivery. These MILs have a high ability to release drugs under acidic conditions, indicating their high efficiency compared to other MILs. In addition to drugs, these nanocarriers can also carry fluorescent, photothermal, and photodynamic agents. These agents allow the MIL nanocarriers to benefit from the therapeutic potential of photothermal and photodynamic agents in addition to the therapeutic capacity of the drug. Furthermore, the fluorescent active ingredient gives these nanocarriers a further tracking capability in addition to the inherent tracking capability of MRI. Therefore, MIL nanocarriers as theranostic carriers have the potential to revolutionize both drug delivery and imaging.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electronic structure modulation of ultrathin PtRuMoCoNi high-entropy alloy nanowires for boosting peroxidase-like activity and sensitive colorimetric determination of isoniazid and hydrazine 超薄PtRuMoCoNi高熵合金纳米线提高过氧化物酶样活性的电子结构调制及异烟肼和联氨的灵敏比色测定

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-15 DOI: 10.1007/s00604-024-06892-2

Jia-Qi Li, Ai-Jun Wang, Pei Song, Jiu-Ju Feng, Qi Zhou, Tuck Yun Cheang

{"title":"Electronic structure modulation of ultrathin PtRuMoCoNi high-entropy alloy nanowires for boosting peroxidase-like activity and sensitive colorimetric determination of isoniazid and hydrazine","authors":"Jia-Qi Li, Ai-Jun Wang, Pei Song, Jiu-Ju Feng, Qi Zhou, Tuck Yun Cheang","doi":"10.1007/s00604-024-06892-2","DOIUrl":"10.1007/s00604-024-06892-2","url":null,"abstract":"<div><p>Self-supported ultrathin PtRuMoCoNi high-entropy alloy nanowires (HEANWs) were synthesized by a one-pot co-reduction method, whose peroxidase (POD)-like activity and catalytic mechanism were elaborated in detail. As expected, the PtRuMoCoNi HEANWs showed excellent POD-like activity. It can quickly catalyze the oxidization of colorless 3,3′,5,5′-tetramethylbenzidine (TMB) to blue <sub>OX</sub>TMB through decomposition of H<sub>2</sub>O<sub>2</sub> to superoxide radicals. Notably, isoniazid and hydrazine effectively scavenge the as-produced superoxide radicals and reduce the blue <sub>OX</sub>TMB, showing high reduction ability and antioxidant property. Thus, the PtRuMoCoNi HEANW-derived colorimetric method was developed for determination of isoniazid and hydrazine, which exhibited the linear ranges of 1.5 to 50 μM and 25 to 200 μM coupled with the lower detection limits of 2.3 and 12.6 μM for isoniazid and hydrazine, respectively. The excellent analytical performance mainly results from the synergistic catalytic effect of the multiple metals and distinctive ultra-thin nanowires. This work provides a simple and rapid colorimetric method for the determination of isoniazid and hydrazine in actual samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fluorescent sensor based on pentafluoropropanoic acid–functionalized UiO-66-NH2 for enhanced selectivity and sensitivity of dicloran detection 一种基于五氟丙烷酸功能化UiO-66-NH2的荧光传感器，用于提高双氯兰检测的选择性和灵敏度。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-15 DOI: 10.1007/s00604-024-06938-5

Xueqin Sun, Yan Fan, Wenyuan Zhang, Xiaogang Yang, Jiacheng Liu

{"title":"A fluorescent sensor based on pentafluoropropanoic acid–functionalized UiO-66-NH2 for enhanced selectivity and sensitivity of dicloran detection","authors":"Xueqin Sun, Yan Fan, Wenyuan Zhang, Xiaogang Yang, Jiacheng Liu","doi":"10.1007/s00604-024-06938-5","DOIUrl":"10.1007/s00604-024-06938-5","url":null,"abstract":"<div><p> A pentafluoropropionic acid–functionalized fluorescent metal–organic framework material (UiO-66-NH<sub>2</sub>-PFPA) is prepared by a simple post-synthetic modification (PSM) strategy for the sensitive and selective detection of dichloran (DCN). The results of fluorescence experiments demonstrate that the sensitivity of UiO-66-NH<sub>2</sub>-PFPA (limit of detection, LOD = 0.478 μM) to DCN is nearly 10.93 times higher than that of UiO-66-NH<sub>2</sub> (LOD = 5.225 μM) and the material has good selectivity and anti-interference ability. After the addition of DCN, the blue fluorescence of UiO-66-NH<sub>2</sub>-PFPA is obviously quenched. Therefore, the possible quenching mechanism is further discussed in combination with relevant experiments and density functional theory calculations. Moreover, the sensor is applied to the detection of DCN in fruit samples with a satisfactory recovery of 101.1—107.9%, which implies that UiO-66-NH<sub>2</sub>-PFPA is expected to be a candidate material for the detection of DCN in food.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sensitized dual-response ratiometric fluorescent sensor integrated smartphone platform for accurate discrimination and detection of tetracycline homologues based on N-CDs‒Eu3+ complex 基于N-CDs-Eu3 +络合物的灵敏双响应比例荧光传感器集成智能手机平台，用于准确鉴别和检测四环素同源物。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-15 DOI: 10.1007/s00604-024-06933-w

Shengnan Yin, Changlun Tong

{"title":"A sensitized dual-response ratiometric fluorescent sensor integrated smartphone platform for accurate discrimination and detection of tetracycline homologues based on N-CDs‒Eu3+ complex","authors":"Shengnan Yin, Changlun Tong","doi":"10.1007/s00604-024-06933-w","DOIUrl":"10.1007/s00604-024-06933-w","url":null,"abstract":"<div><p> A sensitized dual-response ratiometric fluorescent sensor integrated smartphone platform for accurate discrimination and detection of tetracycline (TC) homologues was fabricated based on N-CDs-Eu<sup>3+</sup> complex. In the sensing system, N-CDs act as a sensitizer of Eu<sup>3+</sup> and significantly enhance the fluorescence of TC-Eu<sup>3+</sup> complex approximate 40-fold owing to the synergistic effect of antenna effect (AE) and fluorescence resonance energy transfer (FRET). A paper sensor integrated with a smartphone platform is further fabricated for on-site measurement of TC. The proposed sensing platform exhibits an obvious color change from blue to red with limit of detection (LOD) of 1.5 and 63.2 nM for spectrofluorimetry and paper sensor, respectively. In addition, to eliminate the interference from TC homologues, a simple and effective sensor array was constructed by regulating the pH of the system. The different fluorescence responses to four tetracycline homologues used widely (TC, OTC, CTC, and DOX) were examined and dealt with principal component analysis, and accurate differentiation of TC homologues was therefore achieved. This work provides an integrated method for identification and synchronous quantitative detection of TCs, and a potential application for visual and on-site detection of TCs in environmental monitoring and food safety.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142982471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Eco-friendly sensing of hexavalent chromium ions via copper-doped carbon quantum dots: a fluorescent probe for water safety 通过铜掺杂碳量子点对六价铬离子的环保传感：一种用于水安全的荧光探针

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-15 DOI: 10.1007/s00604-024-06939-4

Shubam Sudan, Jyotsna Kaushal, Thakur Gurjeet Singh, Mohamed H. Mahmoud, Athanasios Alexiou, Marios Papadakis, Mohammed E. Abo-El Fetoh, Gaber El-Saber Batiha

{"title":"Eco-friendly sensing of hexavalent chromium ions via copper-doped carbon quantum dots: a fluorescent probe for water safety","authors":"Shubam Sudan, Jyotsna Kaushal, Thakur Gurjeet Singh, Mohamed H. Mahmoud, Athanasios Alexiou, Marios Papadakis, Mohammed E. Abo-El Fetoh, Gaber El-Saber Batiha","doi":"10.1007/s00604-024-06939-4","DOIUrl":"10.1007/s00604-024-06939-4","url":null,"abstract":"<div><p>The hydrothermal synthesis is presented of copper-doped carbon dots (Cu-CDs) from citric acid, urea, and copper chloride, resulting in blue-fluorescent particles with stable emission at 438 nm when excited at 340 nm. Through comprehensive spectroscopic and microscopic characterization (FTIR, XPS, UV, and HRTEM), the Cu-CDs demonstrated remarkable stability across varying pH levels, ionic strengths, temperatures, and UV exposure. Notably, Cu-CDs exhibit ultra-sensitive and selective detection of hexavalent chromium [Cr(VI)] ions in aqueous environments driven by fluorescence quenching. The system showed a robust linear response to Cr(VI) in the 0–80 µM range, with an impressive limit of detection (LOD) of 0.186 µM, significantly lower than the WHO’s permissible limit of 0.96 µM. These findings position Cu-CDs as an effective tool for environmental monitoring and water safety applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06939-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142994416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzyme cascade nanozyme based colorimetric sensor for detection of uric acid as a biomarker of hyperuricemia 酶级联纳米酶比色传感器检测尿酸作为高尿酸血症的生物标志物

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-15 DOI: 10.1007/s00604-024-06946-5

Wenrui Ke, Guochen Kuang, Chuangqi Gu, Junhao Wang, Xiao Jiang, Rongjiao Zhu, Haiyuan Wang, Zhen Zhang

{"title":"Enzyme cascade nanozyme based colorimetric sensor for detection of uric acid as a biomarker of hyperuricemia","authors":"Wenrui Ke, Guochen Kuang, Chuangqi Gu, Junhao Wang, Xiao Jiang, Rongjiao Zhu, Haiyuan Wang, Zhen Zhang","doi":"10.1007/s00604-024-06946-5","DOIUrl":"10.1007/s00604-024-06946-5","url":null,"abstract":"<div><p> A Cr-doped VO<sub>2</sub> nanobelt (Cr/VO<sub>2</sub>) with remarkable peroxidase-like activity was synthesized and coupled with uricase to catalyze the cascade reaction for detection of uric acid. Notably, the affinity of Cr/VO<sub>2</sub> for 3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate (TMB) and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is tenfold and 20-fold higher, respectively, than that of horseradish peroxidase (HRP). The Cr/VO<sub>2</sub> exhibits highly reactive and stable peroxidase activity at temperatures of 20-60 ℃. Thus, the reaction catalyzed by Cr/VO<sub>2</sub> can be carried out at room temperature, greatly simplifying the testing process. This enzyme cascade nanozyme based colorimetric assay enables the selective and simple detection of uric acid within 30 min with a detection limit of only 0.34 μM, and has an observable potential in clinical applications.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06946-5.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel hierarchical porous biochar based on ZIF-8 volatile hard template with high-efficiency electrochemical sensing performance for trace determination of Ponceau 4R 基于ZIF-8挥发性硬模板的高效电化学传感层次化多孔生物炭用于痕量Ponceau 4R的测定

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-15 DOI: 10.1007/s00604-025-06960-1

Qi Yu, Jing Zhou, Wenhai Wang, Rumin Fu, Wentao Yan, Hui Chen, Yansha Gao, Guorong Fan, Linyu Wang, Zongde Wang, Limin Lu

{"title":"A novel hierarchical porous biochar based on ZIF-8 volatile hard template with high-efficiency electrochemical sensing performance for trace determination of Ponceau 4R","authors":"Qi Yu, Jing Zhou, Wenhai Wang, Rumin Fu, Wentao Yan, Hui Chen, Yansha Gao, Guorong Fan, Linyu Wang, Zongde Wang, Limin Lu","doi":"10.1007/s00604-025-06960-1","DOIUrl":"10.1007/s00604-025-06960-1","url":null,"abstract":"<div><p>A convenient method is proposed using a heat-treatable volatile template to prepare hierarchical porous biochar (HPB). <i>Litsea cubeba</i> leaves and ZIF-8 served as carbon source and volatile hard template, respectively. The good compatibility between ZIF-8 and biomass facilitated their uniform dispersion, and the thermal decomposition of ZIF-8 created abundant pores in the HPB. By using ZIF-8 with different sizes as “modulators”, the textural properties of HPB can be controlled. The HPB derived from 200 nm-sized ZIF-8 exhibited ultra-high specific surface area, rich macro-meso-micro porous structure, and excellent electrocatalytic activity, which makes HPB-200 an effective electrochemical sensing platform. The sensor demonstrated an ultralow detection limit (0.2 nM) of Ponceau 4R, showing significant application potential in the field of electroanalytical sensing.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div><div><p>Schematic illustration of a convenient method using a heat-treatable volatile template to prepare hierarchical porous biochar (HPB)</p></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A SERS/colorimetric biosensor based on AuNSs@Ag core–shell Prussian blue nanozyme for non-interference and rapid detection of Staphylococcus aureus in milk 基于AuNSs@Ag核壳普鲁士蓝纳米酶的SERS/比色生物传感器用于牛奶中金黄色葡萄球菌的无干扰快速检测

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-15 DOI: 10.1007/s00604-024-06921-0

Yajun Zhao, Ming Guan, Fang Mi, Yiyao Zhang, Pengfei Geng, Shan Zhang, Han Song, Guotong Chen

{"title":"A SERS/colorimetric biosensor based on AuNSs@Ag core–shell Prussian blue nanozyme for non-interference and rapid detection of Staphylococcus aureus in milk","authors":"Yajun Zhao, Ming Guan, Fang Mi, Yiyao Zhang, Pengfei Geng, Shan Zhang, Han Song, Guotong Chen","doi":"10.1007/s00604-024-06921-0","DOIUrl":"10.1007/s00604-024-06921-0","url":null,"abstract":"<div><p>A AuNSs@PB@Ag-Apt surface-enhanced Raman scattering (SERS) probe has been developed by embedding Prussian blue (PB) between Au core and Ag shell. The PB SERS probe illustrates strong SERS activity in the Raman silent region of 2070 cm<sup>−1</sup>, and has a zero background signal, ensuring high sensitivity for the detection of <i>Staphylococcus aureus </i>(<i>S.</i> <i>aureus</i>). Moreover, the assemble SERS probe exhibits the catalytic function of peroxidase (POD), enabling the catalysis of 3,3′,5,5′-tetramethylbenzidine (TMB) for colorimetric detection. Additionally, we prepared lectin-functionalized magnetic nanoparticles (F-MNPs-ConA) that demonstrate superior adsorption capabilities towards <i>S. aureus</i>, achieving an impressive adsorption value of up to 98.62%. The F-MNPs-ConA/<i>S. aureus</i>/AuNSs@PB@Ag-Apt sandwich structure constructed by the above SERS probe and magnetic nanoparticles achieves highly sensitive detection of <i>S. aureus</i>. The detection range is 1 × 10<sup>0</sup> ~ 1 × 10<sup>8</sup> CFU·mL<sup>−1</sup>, and the detection limit (LOD) is 1 CFU·mL<sup>−1</sup>. Notably, this method can be integrated into smartphones, facilitating rapid and on-site detection of <i>S. aureus</i>. Compared with similar biosensors, this biosensor offers distinct advantages, including a wide detection range and a low detection limit. The reliability of the biosensor was confirmed by its application in milk, where the recovery ranged from 93.66 to 120.8%. The dual-mode SERS/colorimetric detection, non-interfering nature, magnetic enrichment, and reusability of the biosensor make it a promising tool for point-of-care testing (POCT).</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06921-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0