Microchimica Acta最新文献_第3页

A facile optical fiber-embedded microfluidic biochip for rapid and sensitive detection of microRNA-let-7a in serum

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-06 DOI: 10.1007/s00604-024-06865-5

Yongkai Lu, Jiaxin Xu, Siyan Liu, Baochun Tian, Feng Long

{"title":"A facile optical fiber-embedded microfluidic biochip for rapid and sensitive detection of microRNA-let-7a in serum","authors":"Yongkai Lu, Jiaxin Xu, Siyan Liu, Baochun Tian, Feng Long","doi":"10.1007/s00604-024-06865-5","DOIUrl":"10.1007/s00604-024-06865-5","url":null,"abstract":"<div><p> A novel hybridization chain reaction (HCR) powered optical fiber-embedded microfluidic biochip (HCR-FMB) has been constructed for ultrafast and sensitive detection of lethal-7a (let-7a) in serum. By integrating HCR, fluorescence energy resonant transfer, and evanescent wave fluorescence principle, the HCR-FMB enables detecting let-7a with satisfactory limit of detection of 100.0 pM within 6 min at room temperature, and demonstrates excellent specificity. The HCR-FMB can directly detect let-7a in serum with high sensitivity and without any pre-treatment, and good recoveries were observed for let-7a in serum samples, demonstrating their potential application to the analysis of serum samples. The HCR-FMB exhibits several advantages, including rapidity, enzyme-free, miniaturization, ease-of-operation, field-deployment applicability, minimal-equipment, and cost-effectiveness. The HCR-FMB can be considered a revolutionary detection device that rapidly adapts and deploys in various settings, especially in low medical resource regions.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a colorimetric/fluorescence dual-mode immunoassay for aflatoxin B1 based on streptavidin-induced gold nanoparticle aggregation

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-06 DOI: 10.1007/s00604-024-06843-x

Yaqian Cheng, Chenxi Wang, Xueyu Chang, Xuexia Jia, Zesheng Liu, Baolin Liu, Zhixian Gao, Huanying Zhou

{"title":"Development of a colorimetric/fluorescence dual-mode immunoassay for aflatoxin B1 based on streptavidin-induced gold nanoparticle aggregation","authors":"Yaqian Cheng, Chenxi Wang, Xueyu Chang, Xuexia Jia, Zesheng Liu, Baolin Liu, Zhixian Gao, Huanying Zhou","doi":"10.1007/s00604-024-06843-x","DOIUrl":"10.1007/s00604-024-06843-x","url":null,"abstract":"<div><p>A dual-mode immunoassay method was developed for colorimetric and fluorescence detection of aflatoxin B1 (AFB1) based on streptavidin-induced gold nanoparticle aggregation (AuNP@SA). AuNP-modified streptavidin–biotin labeling AFB1 complete antigen aggregations (AuNP@SA@Bio-BSA-AFB1) were synthesized as the competitive binding and dual-mode probe. AuNP@SA@Bio-BSA-AFB1 aggregations possessed high colorimetric and fluorescence quenching intensities. AFB1 antibodies modified immunomagnetic microspheres were used as the capture probe. The competitive binding between AFB1 and AuNP@SA@Bio-BSA-AFB1 leads to changes in color and fluorescence intensity. The detection limit of the colorimetric method is 6.95 ng·mL<sup>−1</sup>, while that of the fluorescence method is 0.07 ng·mL<sup>−1</sup>. The practicality of the proposed strategy was demonstrated by determining AFB1 in spiked peanut samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142789208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile and rapid preparation of fluorinated imprinted adsorbent for magnetic solid phase extraction of liquid–crystal monomers

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-06 DOI: 10.1007/s00604-024-06851-x

YueYue Zhang, Dingliang Tang, Yuanfei Wu, Xiaojia Huang

{"title":"Facile and rapid preparation of fluorinated imprinted adsorbent for magnetic solid phase extraction of liquid–crystal monomers","authors":"YueYue Zhang, Dingliang Tang, Yuanfei Wu, Xiaojia Huang","doi":"10.1007/s00604-024-06851-x","DOIUrl":"10.1007/s00604-024-06851-x","url":null,"abstract":"<div><p> A new fluorinated imprinted adsorbent (MIA) for magnetic solid phase extraction (MSPE) of liquid crystal monomer (LCM) pollutants was one-pot prepared within 3.5 h using 4-[difluoro(3,4,5-trifluorophenoxy)methyl]-3,5-difluoro-4′-ethyl-biphenyl (DFBP) as template and 1H,1H,2H,2H-heptadecafluorodecyl acrylate/vinylanthracene as dual monomers. The structure, morphology, and magnetic properties of MIA fabricated were investigated by various characterization techniques. Under the optimal conditions the prepared MIA presented satisfactory specific recognition performance. The recognition factor and adsorption capacity towards DFBP were 2.7 and 15.9 mg/g, respectively. The specific recognition behaviors of MIA/MSPE towards DFBP were surveyed by means of adsorption kinetics and adsorption isotherms. Combining MSPE with HPLC coupled to a diode array detector (DAD), a sensitive, reliable and anti-interference method for the monitoring of LCMs residuals in various environmental water and soil samples was established. The achieved enrichment factors were 132–248 and 96–204 in water and soil samples, respectively. The corresponding limits of detection were 0.0017–0.0051 μg/L and 0.087–0.28 μg/kg, respectively. Moreover, confirmatory experiments were adopted to inspect the accuracy of the established MIA/MSPE-HPLC/DAD approach. To the best of our knowledge, this is the first time that an imprinted material has been used for specific isolation and capture of LCMs which have been classified as emerging organic pollutants.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142789209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple capillary isoelectric focusing method as the novel strategy for the isoelectric point measurement of exosomes and its application in disease diagnosis

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-05 DOI: 10.1007/s00604-024-06864-6

Kaige Yang, Wenchang Fu, Yinjie Ma, Mingyuan Wu, Xinyan Li, Yan Wang

{"title":"A simple capillary isoelectric focusing method as the novel strategy for the isoelectric point measurement of exosomes and its application in disease diagnosis","authors":"Kaige Yang, Wenchang Fu, Yinjie Ma, Mingyuan Wu, Xinyan Li, Yan Wang","doi":"10.1007/s00604-024-06864-6","DOIUrl":"10.1007/s00604-024-06864-6","url":null,"abstract":"<div><p>A straightforward capillary isoelectric focusing (cIEF) method is established with a isoelectric point (pI) range spanning from 3.5 to 7.0, exhibiting excellent linearity and repeatability, with an R<sup>2</sup> value of 0.9937 and migration time RSDs for all standard proteins below 0.3%. Subsequently, this method was applied to model exosomes derived from cell lines and healthy human serum, and the peak attributions of exosomes were identified using DiI labeled exosomes and lysed exosomes. The reproducibility of this method in exosome detection was also validated, as the RSDs of all pI values were less than 1%. Then, we observed a significant increase in the pI of exosomes with higher cholesterol content, irrespective of whether they originated from cell culture medium or mouse plasma. Notably, serum exosomes from healthy volunteers exhibited higher pI values compared to those from hepatocellular carcinoma patients, suggesting a potential diagnostic perspective for cancer. These findings underscore the significance of pI measurement in reflecting modifications in exosomal lipid membrane composition and their implications in biological functions mediated by exosomes.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modified Carba PBP test for rapid detection and differentiation between different classes of carbapenemases in Enterobacterales

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-05 DOI: 10.1007/s00604-024-06859-3

Xiaonan Wang, Zhimin Lu, Leina Dou, Licai Ma, Tong He, Chenxi Gao, Xiangjun Zhao, Jin Tao, Liang Luo, Qing Li, Yang Wang, Yingbo Shen, Jianzhong Shen, Zhanhui Wang, Kai Wen

{"title":"Modified Carba PBP test for rapid detection and differentiation between different classes of carbapenemases in Enterobacterales","authors":"Xiaonan Wang, Zhimin Lu, Leina Dou, Licai Ma, Tong He, Chenxi Gao, Xiangjun Zhao, Jin Tao, Liang Luo, Qing Li, Yang Wang, Yingbo Shen, Jianzhong Shen, Zhanhui Wang, Kai Wen","doi":"10.1007/s00604-024-06859-3","DOIUrl":"10.1007/s00604-024-06859-3","url":null,"abstract":"<div><p>An advanced biochemical assay named modified Carba PBP test was innovated to identify and differentiate distinct categories of clinically significant carbapenemases (Ambler classes A, B, and D) within the <i>Enterobacterales</i>. The mechanism of mCarba PBP hinges on two core attributes: (i) the hydrolysis of the meropenem substrate by various carbapenemases, (ii) the immobilized penicillin and free meropenem in their affinity to interact with a limited quantity of penicillin-binding protein (PBP). Specific inhibitors for class A (phenylboronic acid, PBA) and class B (ethylenediaminetetraacetic acid, EDTA) were employed to inhibit the hydrolysis activity of carbapenemase and facilitate the classification of carbapenemase classes within 25 min. A comprehensive evaluation was undertaken using 94 clinical <i>Enterobacterales</i> isolates, comprising 75 carbapenemase-producing strains and 19 non-carbapenemase-producing strains. Its overall specificity and sensitivity were 100% and 97.3%, respectively, including detection of all types of OXA-48-like carbapenemases. For precise carbapenemase type identification, the assay exhibited remarkable sensitivities for class A, class B, and class D detection at 94.7%, 100%, and 100%, respectively. This user-friendly test presents a promising tool for carbapenemase identification, refining the selection of β-lactam/β-endoenzyme inhibitor combinations for effectively treating infections due to carbapenemase-producing organisms.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142778372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Aβ40 in cerebrospinal fluid and plasma of Alzheimer’s disease patients using photoelectrochemical biosensors

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-04 DOI: 10.1007/s00604-024-06816-0

Liting Li, Na Wei, Yiwei Guo, Xingyu Zhu, Lin Wang, Yanzhao Zhu, Ke Fang, Shenhui Ma, Yingying Zhang, Yan Zhang, Xiaofei Zhou, Gang Zhao, Yuyu Bu, Linfu Zhou

{"title":"Detection of Aβ40 in cerebrospinal fluid and plasma of Alzheimer’s disease patients using photoelectrochemical biosensors","authors":"Liting Li, Na Wei, Yiwei Guo, Xingyu Zhu, Lin Wang, Yanzhao Zhu, Ke Fang, Shenhui Ma, Yingying Zhang, Yan Zhang, Xiaofei Zhou, Gang Zhao, Yuyu Bu, Linfu Zhou","doi":"10.1007/s00604-024-06816-0","DOIUrl":"10.1007/s00604-024-06816-0","url":null,"abstract":"<div><p>An ultra-sensitive photoelectrochemical (PEC) biosensor for amyloid-beta 40 (Aβ40), a biomarker for Alzheimer’s disease (AD), was developed using g-C₃N₄ modified with gold nanoparticles (Au NPs) to form Au-C₃N₄. This was further combined with TiO₂ to create a tightly bonded TiO₂/Au-C₃N₄ heterojunction, leading to a highly responsive photocatalytic process. Furthermore, the incorporation of noble metal Au NPs not only enhances photocurrent generation but also securely immobilizes the aptamer through Au–S bonds, providing additional surface binding sites. This significantly increases the sensor's capture efficiency. The sensor exhibited excellent performance, featuring a linear detection range from 10<sup>−15</sup> to 10<sup>−11</sup> g/mL and a remarkably low detection limit (LOD) of 0.33 fg/mL. Moreover, the validation in clinical settings demonstrated the successful detection in real cerebrospinal fluid (CSF) and plasma, from AD patients and non-AD controls. These results strongly suggest that PEC biosensors possess significant potential as cost-effective and highly sensitive tools for detecting ultra-trace substances in human body fluids, which offers promising opportunities for the early screening of high-risk populations for AD.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Molecularly imprinted electrochemical sensor based on APTES-functionalized indium tin oxide electrode for the determination of sulfadiazine

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-04 DOI: 10.1007/s00604-024-06866-4

Samridhi Chopra, Manisha Balkhandia, Manisha Khatak, Navya Sagar, Ved Varun Agrawal

引用次数: 0

Electrochemical immunosensing of Crohn’s disease biomarkers using diazonium salt-grafted crystalline nanocellulose/carbon nanotube-modified electrodes

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-04 DOI: 10.1007/s00604-024-06868-2

Lorena García-Rodrigo, Claudia Ramos-López, Esther Sánchez-Tirado, Lourdes Agüí, Araceli González-Cortés, Paloma Yáñez-Sedeño, José M. Pingarrón

{"title":"Electrochemical immunosensing of Crohn’s disease biomarkers using diazonium salt-grafted crystalline nanocellulose/carbon nanotube-modified electrodes","authors":"Lorena García-Rodrigo, Claudia Ramos-López, Esther Sánchez-Tirado, Lourdes Agüí, Araceli González-Cortés, Paloma Yáñez-Sedeño, José M. Pingarrón","doi":"10.1007/s00604-024-06868-2","DOIUrl":"10.1007/s00604-024-06868-2","url":null,"abstract":"<div><p>The first dual immunosensor is reported for the determination of IL-12 and IL-23, two relevant biomarkers of Crohn’s disease (CD). The strategy relies on the selective capture of the targets by the respective antibodies which were covalently immobilized onto SPCEs modified with crystalline nanocellulose (CNC) and multi-walled carbon nanotubes (MWCNTs) followed by conjugation with a detector antibody labelled with poly-HRP-Strept and amperometric transduction using the H<sub>2</sub>O<sub>2</sub>/HQ system. The combination of CNC, a nanomaterial scarcely exploited in immunosensing, with MWCNTs enables the preparation of a novel dual immunosensor for the determination of CD biomarkers in clinical samples, including faeces. The developed bioplatform exhibits a high reproducibility and selectivity allowing the simultaneous determination of IL-12 and IL-23 using a smaller sample volume and in a much shorter assay time than those reported for each target with commercial ELISA kits. The dual immunosensor was successfully applied to the analysis of raw serum and faeces spiked with IL-12 and IL-23 at the expected levels in patients with severe CD.\u0000</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142765262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical sensor based on molecularly imprinted polypyrrole-MWCNTs-OH/covalent organic framework for the detection of ofloxacin in water

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-03 DOI: 10.1007/s00604-024-06860-w

Lijuan Huang, Yuan Luo, Xulin Li, Juan Wu, Qian Long, Li Zheng, Wenlong Liao, Huiming Li, Lingpu Jia, Kunping Liu

{"title":"Electrochemical sensor based on molecularly imprinted polypyrrole-MWCNTs-OH/covalent organic framework for the detection of ofloxacin in water","authors":"Lijuan Huang, Yuan Luo, Xulin Li, Juan Wu, Qian Long, Li Zheng, Wenlong Liao, Huiming Li, Lingpu Jia, Kunping Liu","doi":"10.1007/s00604-024-06860-w","DOIUrl":"10.1007/s00604-024-06860-w","url":null,"abstract":"<div><p>A platform was developed to accurately detect the content of ofloxacin (OFX) based on molecularly imprinted polypyrrole-MWCNTs-OH/1,3,5-Tris(4-aminophenyl) benzene (TAPB)-2,5-dimethoxybenzene-1,4-dicarboxaldehyde (DMTP)-covalent organic framework (MIP-MWCNTs-OH/COF)–modified glassy carbon electrode (GCE) sensor (MIP-MWCNTs-OH/COF/GCE). The complex of MWCNTs-OH and COF synergistically enhanced the active area and electrochemical signal, based on which a molecularly imprinted membrane was polymerized on its surface to further improve the selectivity. Under optimized conditions, the prepared MIP-MWCNTs-OH/COF/GCE sensor exhibited strong detection performance to OFX in a linear range 1.969 × 10<sup>−11</sup>–9.619 × 10<sup>−9</sup> M with the limit of detection (LOD, 3S/N) of 4.989 × 10<sup>−12</sup> M, excellent selectivity, stability, and reproducibility. Furthermore, the MIP-MWCNTs-OH/COF/GCE sensor can be successfully applied to the detection of OFX in lake water and eye drops with a relative standard deviation (RSD) of less than 4.95%, indicating its high potential in practical applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142761919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Colorimetric sensor array for sensitive detection and identification of bacteria based on the etching of triangular silver nanoparticles regulated by Cl− at various concentrations

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-12-02 DOI: 10.1007/s00604-024-06855-7

Shuang Liu, Peng Yan, Shahzad Naveed, Yuheng Zhu, Tao Fu, Ruijing Wu, Yayan Wu

{"title":"Colorimetric sensor array for sensitive detection and identification of bacteria based on the etching of triangular silver nanoparticles regulated by Cl− at various concentrations","authors":"Shuang Liu, Peng Yan, Shahzad Naveed, Yuheng Zhu, Tao Fu, Ruijing Wu, Yayan Wu","doi":"10.1007/s00604-024-06855-7","DOIUrl":"10.1007/s00604-024-06855-7","url":null,"abstract":"<div><p>A colorimetric sensor array is proposed for ultrasensitive detection and identification of bacteria by using Cl<sup>−</sup> at various concentrations as sensing elements and triangular silver nanoparticles (T-AgNPs) as a single sensing nanoprobe. T-AgNPs are easily etched by Cl<sup>−</sup>. However, in the presence of bacteria, the etching process will be hindered. Different bacteria have differential protective effects on T-AgNPs due to their interactions, resulting in different etching degrees of T-AgNPs by Cl<sup>−</sup>, and visual color changes. By adjusting the antagonistic action between bacteria protection on T-AgNPs and the etching by using Cl<sup>−</sup> at various concentrations, different bacteria had their own color response patterns. Combined with linear discriminant analysis (LDA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), the bacteria could be identified. The method was also used for bacteria mixtures identification and showed high sensitivity (OD<sub>600</sub> = 1.0 × 10<sup>−6</sup>) for <i>V. parahaemolyticus</i> detection. Finally, the sensor array was successfully utilized in the identification of bacteria in pure and mineral bottled water. The method is low-cost, simple, sensitive, visual, and has potential application in point-of-care testing of bacteria.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142758000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0