Microchimica Acta最新文献

{"title":"Construction of highly emissive Schiff base containing TX- 100 nanomicelles for monitoring the levels of devil in disguise Allura red and picric acid in real samples","authors":"Nisha Jain,&nbsp;Navneet Kaur","doi":"10.1007/s00604-025-07166-1","DOIUrl":"10.1007/s00604-025-07166-1","url":null,"abstract":"<div><p>A sulfonamide derivative synthesized from 1,8-naphthalimide-based amine and 4-methoxy-benzenesulfonyl chloride has been enwrapped into the micelles of a neutral surfactant TX-100 to form highly emissive nanomicelles <b>MXL@TX- 100</b>. The proposed sensor offered highly sensitive turn-off detection of Allura red (linear range = 0.66–224 µM) with the limit of detection (LOD) of 0.2 µM (<i>R</i>² = 0.999) due to fluorescence resonance energy transfer from <b>MXL@TX- 100</b> to Allura red. To the best of our knowledge, no organic compound-based sensor has been reported for the determination of Allura red so far. Additionally, nanomicelles <b>MXL@TX- 100</b> were utilized for the sensing of picric acid (LOD = 0.92 µM, <i>R</i>² = 0.998) in the linear range 3.03–928 µM. The binding constant between <b>MXL@TX- 100-</b>Allura red and <b>MXL@TX- 100-</b>picric were calculated to be 1.45 × 10⁵ M⁻¹ and 1.97 × 10⁴ M⁻¹, respectively. Moreover, nanomicelles <b>MXL@TX- 100</b> conveniently detected Allura red in real samples, viz. candy, soft drink, gulkand, and pharmaceutical syrup.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A ruthenium-nickel metal–organic framework incorporating 2,2'-bipyridine- 5,5'-dicarboxylic acid for dual-mode electrochemiluminescence-fluorescence detection of ferric ions","authors":"Jing Zhang,&nbsp;Zhaojiang Yin,&nbsp;Wei Xiong,&nbsp;QiQi Fan,&nbsp;Fengyao Yu,&nbsp;Fusheng Liao,&nbsp;Hao Fan,&nbsp;Fei Qu,&nbsp;Qiangqiang Yu","doi":"10.1007/s00604-025-07168-z","DOIUrl":"10.1007/s00604-025-07168-z","url":null,"abstract":"<div><p>A novel, low-toxicity Ru-Ni-BPY metal–organic framework (MOF) nanomaterial was developed and a simple and sensitive dual-mode \"on–off\" ferric ions (Fe³⁺) sensing platform based on this material constructed. The dual-mode detection method enabled cross-validation of the electrochemiluminescence (ECL) and fluorescent (FL) signals, enhancing accuracy and effectively minimizing errors. In linear ranges of 0.005–10 μM and 0.078–10 μM, Fe³⁺ can dramatically quench the ECL and FL signals of the Ru-Ni-BPY MOF, with quenching rates of 91.42% and 91.31%, respectively, and detection limits of 0.1 nM and 9.3 nM, respectively. This ECL method not only achieved efficient detection of Fe³⁺ in rat brain microdialysis fluid but also successfully enabled real-time FL detection of Fe³⁺ in nude mice. Therefore, this platform has great potential for applications in environmental protection, life sciences, and early disease diagnosis.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-performance electrochemical immunosensor for ultrasensitive detection of malachite green in food matrices using MOF-derived nanocomposites","authors":"Na Li,&nbsp;Yiqun Guo,&nbsp;Mengmeng Wu,&nbsp;Aiying Wang,&nbsp;Yuna Guo,&nbsp;Yuancheng Li","doi":"10.1007/s00604-025-07170-5","DOIUrl":"10.1007/s00604-025-07170-5","url":null,"abstract":"<div><p> A highly specific and ultrasensitive electrochemical immunosensor for malachite green (MG) detection in complex food matrices is presented. The sensor was constructed through a stepwise assembly process incorporating gold nanoparticles, antibodies, and metal–organic framework (MOF)-derived Pd/CuO@NiO nanocomposites. Its structure and electrochemical performance were thoroughly validated using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Key detection parameters, including pH and nanocomposite concentration, were systematically optimized to maximize sensor performance. The sensor demonstrated a broad linear detection range (10⁻⁴ to 100 ng/mL) and an ultralow detection limit (42 pg/L). Specificity tests confirmed the immunosensor’s ability to selectively detect MG without interference from structurally similar compounds such as crystal violet, nitrofuran drugs, and chloramphenicol. Its practical applicability was verified using pretreated freshwater fish samples spiked with MG, yielding recoveries of 95%–105%. Furthermore, the results showed strong agreement with those of the conventional enzyme-linked immunosorbent assays (ELISA) method, with minimal difference ratios observed. These findings establish the immunosensor as a reliable, accurate, and rapid tool for detecting MG in food safety applications. Looking ahead, the platform’s modular design and versatility provide opportunities to extend its application to other harmful contaminants in food and environmental monitoring, contributing to broader advancements in public health and safety.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143856678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel ionic-liquid-supported polythieno[3,2-b]thiophene coating for headspace solid-phase microextraction of phenolic compounds from environmental water via gas chromatography-mass spectrometry","authors":"Ling Shi,&nbsp;Anping Li,&nbsp;Yuying Xu,&nbsp;Hongping Yang,&nbsp;Guangming Yang","doi":"10.1007/s00604-025-07157-2","DOIUrl":"10.1007/s00604-025-07157-2","url":null,"abstract":"<div><p>A novel thieno[3,2-b]thiophene (TT) was utilized for the first time to electrochemically fabricate an ionic liquid (IL)-supported polythieno[3,2-b]thiophene (PTT) coating. This innovative coating serves as a new headspace solid-phase microextraction (HS-SPME) material for the extraction of five phenolic compounds, which were subsequently determined via gas chromatography-mass spectrometry (GC–MS). The coating was characterized using Fourier-transform infrared spectroscopy, scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Experimental results confirmed that the ILs were successfully embedded within the polymer matrix, resulting in a well-defined three-dimensional mesoporous architecture. This unique architecture not only exhibited high thermal stability but also maintained a consistent extraction performance when used for the solid-phase microextraction of phenolic compounds. To optimize the extraction and detection performance, various experimental parameters were investigated, including the coating type, pH, salt concentration, extraction time, extraction temperature, stirring rate, desorption time, and temperature. Under the optimal conditions identified, the method exhibited low limits of detection ranging from 0.001 to 0.007 μg mL⁻¹ and wide linearity in the concentration range 0.030 to 10.000 μg mL⁻¹. Noteworthily, the proposed method was successfully applied to the determination of five phenols in actual water samples, achieving satisfactory recoveries between 90.5% and 110.5%‌.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sandwich-like electrochemical immunosensing of IL-17 based on silver nanoparticles/graphdiyne and methylene blue/UiO-66-NH2","authors":"Rui Li,&nbsp;Wenzhong Que,&nbsp;Haiyan Han,&nbsp;Honghui Shi,&nbsp;Rongfeng Wang,&nbsp;Bin Qiu","doi":"10.1007/s00604-025-07064-6","DOIUrl":"10.1007/s00604-025-07064-6","url":null,"abstract":"<div><p>IL-17 (interleukin-17) is a common proinflammatory cytokine and can be adopted as an important biomarker for several diseases; it is thus very necessary to design an effective method for monitoring IL-17. Herein, by preparing silver nanoparticles/graphdiyne (Ag/GDY) with simple electroless deposition as the sensing platform and methylene blue/UiO-66-NH₂ MOFs (MB/MOFs) as the signaling-amplifier, a highly efficient sandwich-like electrochemical immunosensing (SLEI) platform was designed for IL-17 detection. The superior electrical property, easy preparation, large surface area, and high stability of Ag/GDY make it a desirable substrate to immobilize primary antibody of IL-17. Meanwhile, the unique structure of UiO-66-NH₂ is beneficial to immobilize MB probe and secondary antibody. By optimizing experimental conditions, the MB current obtained at the as-designed SLEI platform increases along with IL-17 increase, and the related linearity is 1.0 pg mL⁻¹–1.0 ng mL⁻¹ coupled with a low detection limit of 0.3 pg mL⁻¹. It is expected that the SLEI platform based on Ag/GDY and MB/MOFs shows some potential application for IL-17 detection.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143845608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid and sensitive detection of malachite green by multifunctional octahedron UiO- 66-NH2/Fe3O4/Ag SERS substrate","authors":"Xiaobo Ma,&nbsp;Fang Mi,&nbsp;Pengfei Geng,&nbsp;Guotong Chen,&nbsp;Shan Zhang,&nbsp;Zhiyuan Sun,&nbsp;Jian Sun,&nbsp;Peng Chen,&nbsp;Ming Guan","doi":"10.1007/s00604-025-07165-2","DOIUrl":"10.1007/s00604-025-07165-2","url":null,"abstract":"<div><p>Multifunctional nanocomposites have attracted extensive attention in the design of new SERS substrates. In this work, a metal-organic framework (MOF) modified with Ag nanoparticles (Ag NPs) and cysteine functionalized Fe₃O₄ (UiO-66-NH₂/Fe₃O₄/Ag) was prepared as a SERS substrate (UFAs). The substrate combines the enrichment ability of UiO-66-NH₂, the magnetic separation ability of Fe₃O₄, and the localized surface plasmon resonance (LSPR) effect of Ag NPs to achieve efficient detection of the target analyte. The results of adsorption kinetics showed that the adsorption process of malachite green (MG) was mainly dominated by chemical adsorption. In addition, we further explored the detection mechanism of UFAs for MG. UFAs enriched cation analytes such as MG through π-π stacking and electrostatic interaction and performed SERS detection. The UFA SERS substrate exhibits good detection sensitivity for MG, with a limit of detection (LOD) of 1.35 × 10⁻¹⁰ M (at 1619 cm⁻¹), and the SERS substrate has good uniformity and stability. The SERS substrate was applied to the detection of MG in aquaculture water. The recovery of MG in the sample was 91.2–105%, and the relative standard deviation (RSD) was 3.76–6.06%. This high-performance UFA SERS substrate also has great potential for the detection of food and other environmental pollutants in practical applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A rare multi-emission metal–organic complex fluorescent probe for direct oxytetracycline recognition","authors":"Zhaojing Yuan,&nbsp;Long Yu,&nbsp;Wenyuan Jiang,&nbsp;Xin Li,&nbsp;Zhiqing Wen,&nbsp;Xiangyang Hao,&nbsp;Mingtai Sun,&nbsp;Suhua Wang","doi":"10.1007/s00604-025-07160-7","DOIUrl":"10.1007/s00604-025-07160-7","url":null,"abstract":"<div><p>A simple and effective metal–organic coordination polymer, EuIn@MOCPs, which enables the rapid and selective detection of oxytetracycline (OTC) among tetracycline antibiotics was successfully synthesized. Unlike the previously reported rare-earth-doped metal–organic complexes, this probe not only exhibits the common 617-nm characteristic peak in response to OTC but also uniquely generates uncommon peak shifts at 591 nm and 652 nm, allowing it to specifically recognize OTC among tetracycline antibiotics. We found that the response of the probe and OTC had a linear relationship with a detection limit as low as 42.3 nM within the 0–90-μM concentration range using multi-peak ratio fluorescence testing. Finally, the rich color change from blue to red in fluorescence makes this probe an excellent candidate for the development of high-performance visual fluorescent test strips. This achievement provides an effective approach for fluorescent probes to recognize structurally similar contaminants.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840403","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preparation of polydentate phosphonate-functionalized adsorbent for selective extraction of rare earth ions in harsh acidic solution","authors":"Xue Chen,&nbsp;Chenyang Wang,&nbsp;Mao Li,&nbsp;Dexiu Zhang,&nbsp;Yinmao Wei","doi":"10.1007/s00604-025-07162-5","DOIUrl":"10.1007/s00604-025-07162-5","url":null,"abstract":"<div><p>Developing adsorbents capable of extracting rare earth element (REE) in harsh acidic solution is a challenging issue in the recycle of REE from secondary resources. In this work, porous polystyrene resin was facile modified with tris(2-aminoethyl)amine followed by methylenebis(phosphonic dichloride) to create a polydentate phosphonate-functionalized adsorbent for this purpose. The adsorption properties of four REE ions (Ce³⁺, Nd³⁺, Gd³⁺, and Dy³⁺) were investigated in terms of affinity constants, selectivity, and recovery. The adsorption mechanism of adsorbent for REE ions is regarded as the strong chelation interaction provided by the phosphonate ligand, which was verified by the experiments of acid and salt effects, thermodynamic and kinetic analysis. By using the diluted digest solution of a permanent magnet as model of strong acidic sample (1 mol/L HCl), the batch adsorption method generates high recovery ranging from 58.1 to 100% toward five REE ions (Y³⁺, Ce³⁺, Nd³⁺, Gd³⁺, and Dy³⁺), while the simulated continuous column bed enhances their total contents from 35.4% in the original digest solution to 88.9% in the eluent. In conclusion, the adsorbent indicates an excellent extraction ability in harsh acidic solution, and the recycle of REE using this adsorbent can predictably reduce alkali consumption to neutralize digest solution of secondary resources and improve greenness in manufacture.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic innovation: MOF@GCN hybrid for electrochemical detection of flutamide—bridging experimental, computational, and real-world applications","authors":"Sirisha Subbareddy,&nbsp;Arehalli Shivamurthy Santhosh,&nbsp;Sahana Kamanna Metry,&nbsp;Kumar Venkatesan,&nbsp;Manickam Selvaraj,&nbsp;Srujan Basavapura Ravikumar,&nbsp;Sandeep Shadakshari","doi":"10.1007/s00604-025-07164-3","DOIUrl":"10.1007/s00604-025-07164-3","url":null,"abstract":"<div><p>Electrochemical sensors are at the forefront of analytical technology, offering remarkable sensitivity and rapid response for detecting a wide range of chemical and biological compounds. Herein, a bimetallic metal–organic framework (MOF) is engineered and combined with graphitic carbon nitride (GCN) to demonstrate exceptional electrochemical performance toward the anti-cancer drug flutamide. A simple solvothermal method is used to synthesize MOF and GCN. These materials are then used as precursors to synthesize the MOF@GCN nanocomposite via a sonication method. The formation of the nanocomposite is confirmed using various characterization techniques like UV–Vis spectroscopy, FTIR spectroscopy, XRD, XPS, TGA, SEM, and TEM. The electrochemical characterization is performed using EIS, and the electrochemical measurements are conducted using CV and LSV. The results obtained from the electrochemical parameters indicate good operational stability, high sensitivity, reliability, and excellent electrochemical conductivity. The LSV curves show linearity over a wide range of flutamide concentration levels (10 to 180 nM), a limit of detection of 17.56 nM, a limit of quantification of 53.23 nM, and an optimal sensitivity of 22.89 µA µM⁻¹ cm⁻². This electrical response of the sensor is attributed to the abundance of active sites, accelerated diffusion, and low rate of recombination. The real sample analysis conducted in biofluids and environmental samples also demonstrate good recovery for the flutamide analyte. The theoretical results obtained from the computational DFT analysis on the analyte are also in good agreement with the experimental results. In a wider perspective, the development of this electrochemical sensor promises significant advancements in health monitoring and environmental protection.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnology-enhanced immunoassay for accurate determination of HT-2 toxin in edible insect samples","authors":"Raúl Sancho-García,&nbsp;Fernando Navarro-Villoslada,&nbsp;Fernando Pradanas-González,&nbsp;Henri O. Arola,&nbsp;Bettina Glahn-Martínez,&nbsp;Tarja K. Nevanen,&nbsp;Elena Benito-Peña","doi":"10.1007/s00604-025-07146-5","DOIUrl":"10.1007/s00604-025-07146-5","url":null,"abstract":"<div><p>Consumption of edible insects is common in non-Western countries of Africa, Asia, Oceania, and Latin America. However, their consumption has significantly increased in Europe in recent years thanks to their remarkable nutritional properties. Edible insects provide a valuable source of high-quality proteins, fats, minerals, and vitamins. Nevertheless, the absence of global regulatory guidelines poses a risk associated with their consumption due to the potential presence of pathogens and contaminants, such as mycotoxins, which are toxic compounds produced by fungi and represent a major threat to food and feed safety. Our approach integrates advanced nanobiotechnology to develop fluorescent antibodies by conjugating a recombinant superfolder green fluorescent protein (sfGFP) with a single-chain antibody (scFv). This fusion allows for precise detection of the immune complex formed between the HT-2 toxin and a biotinylated anti-HT-2 antibody. Additionally, we employed advanced computational tools, including AlphaFold and MOE, to deepen our understanding of the binding interactions present in the immune complex, confirming the strong interaction between the Fab/HT-2 toxin immunocomplex and the scFv antibody fragment, in contrast to the weaker binding observed with the Fab/T-2 toxin and the scFv. The method demonstrates high sensitivity, with an EC₅₀ of 10.3 ± 0.6 ng mL⁻¹, a dynamic range of 3.4 ± 0.1 to 31 ± 3 ng mL⁻¹, a limit of detection of 0.43 ng mL⁻¹, and a limit of quantification of 1.2 ng mL⁻¹ in buffer solution. The assay exhibited excellent precision, with a reproducibility of 4% and no cross-reactivity with other mycotoxins. Application to contaminated cricket flour yielded recoveries between 91 and 133%, with coefficients of variation from 6 to 13%. These results indicate that the developed immunoassay is highly sensitive, selective, and reliable for detecting HT-2 toxin in food matrices, providing a promising tool for mycotoxin screening in food safety.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143840418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}