Sandwich-like electrochemical immunosensing of IL-17 based on silver nanoparticles/graphdiyne and methylene blue/UiO-66-NH2

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-04-17 DOI:10.1007/s00604-025-07064-6

Rui Li, Wenzhong Que, Haiyan Han, Honghui Shi, Rongfeng Wang, Bin Qiu

{"title":"Sandwich-like electrochemical immunosensing of IL-17 based on silver nanoparticles/graphdiyne and methylene blue/UiO-66-NH2","authors":"Rui Li, Wenzhong Que, Haiyan Han, Honghui Shi, Rongfeng Wang, Bin Qiu","doi":"10.1007/s00604-025-07064-6","DOIUrl":null,"url":null,"abstract":"<div><p>IL-17 (interleukin-17) is a common proinflammatory cytokine and can be adopted as an important biomarker for several diseases; it is thus very necessary to design an effective method for monitoring IL-17. Herein, by preparing silver nanoparticles/graphdiyne (Ag/GDY) with simple electroless deposition as the sensing platform and methylene blue/UiO-66-NH<sub>2</sub> MOFs (MB/MOFs) as the signaling-amplifier, a highly efficient sandwich-like electrochemical immunosensing (SLEI) platform was designed for IL-17 detection. The superior electrical property, easy preparation, large surface area, and high stability of Ag/GDY make it a desirable substrate to immobilize primary antibody of IL-17. Meanwhile, the unique structure of UiO-66-NH<sub>2</sub> is beneficial to immobilize MB probe and secondary antibody. By optimizing experimental conditions, the MB current obtained at the as-designed SLEI platform increases along with IL-17 increase, and the related linearity is 1.0 pg mL<sup>−1</sup>–1.0 ng mL<sup>−1</sup> coupled with a low detection limit of 0.3 pg mL<sup>−1</sup>. It is expected that the SLEI platform based on Ag/GDY and MB/MOFs shows some potential application for IL-17 detection.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07064-6","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

IL-17 (interleukin-17) is a common proinflammatory cytokine and can be adopted as an important biomarker for several diseases; it is thus very necessary to design an effective method for monitoring IL-17. Herein, by preparing silver nanoparticles/graphdiyne (Ag/GDY) with simple electroless deposition as the sensing platform and methylene blue/UiO-66-NH₂ MOFs (MB/MOFs) as the signaling-amplifier, a highly efficient sandwich-like electrochemical immunosensing (SLEI) platform was designed for IL-17 detection. The superior electrical property, easy preparation, large surface area, and high stability of Ag/GDY make it a desirable substrate to immobilize primary antibody of IL-17. Meanwhile, the unique structure of UiO-66-NH₂ is beneficial to immobilize MB probe and secondary antibody. By optimizing experimental conditions, the MB current obtained at the as-designed SLEI platform increases along with IL-17 increase, and the related linearity is 1.0 pg mL⁻¹–1.0 ng mL⁻¹ coupled with a low detection limit of 0.3 pg mL⁻¹. It is expected that the SLEI platform based on Ag/GDY and MB/MOFs shows some potential application for IL-17 detection.

Graphical abstract

查看原文本刊更多论文

基于银纳米颗粒/石墨炔和亚甲基蓝/UiO-66-NH2的IL-17的三明治状电化学免疫传感

IL-17（白细胞介素-17）是一种常见的促炎细胞因子，可作为多种疾病的重要生物标志物；因此，设计一种有效的IL-17监测方法是非常必要的。本文以简单化学沉积法制备银纳米颗粒/石墨烯（Ag/GDY）为传感平台，以亚甲基蓝/UiO-66-NH2 mof （MB/ mof）为信号放大器，设计了一种高效的三明治状电化学免疫传感（SLEI）检测IL-17平台。Ag/GDY具有优良的电学性能、制备简单、表面积大、稳定性高等特点，是固定化IL-17一抗的理想底物。同时，UiO-66-NH2独特的结构有利于固定MB探针和二抗。通过优化实验条件，在所设计的SLEI平台上获得的MB电流随着IL-17的增加而增加，相关线性关系为1.0 pg mL−1 ~ 1.0 ng mL−1，检测限低至0.3 pg mL−1。预计基于Ag/GDY和MB/ mof的SLEI平台在IL-17检测中具有一定的应用潜力。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.