Microchimica Acta最新文献_第4页

Visual detection of kanamycin with functionalized Au nanoparticles 功能化金纳米颗粒对卡那霉素的目视检测

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-14 DOI: 10.1007/s00604-025-06954-z

Xigui Liu, Danlian Huang, Cui Lai, Fei Wang

{"title":"Visual detection of kanamycin with functionalized Au nanoparticles","authors":"Xigui Liu, Danlian Huang, Cui Lai, Fei Wang","doi":"10.1007/s00604-025-06954-z","DOIUrl":"10.1007/s00604-025-06954-z","url":null,"abstract":"<div><p>A simple and rapid colorimetric detection strategy, based on hydrogen bond identification of 6-thioguanine (6-TG) functionalized Au nanoparticles (AuNPs), is proposed for highly selective and sensitive determination of kanamycin (KA). In this strategy, the hydrogen bond interaction between 6-TG and kanamycin induces AuNPs to agglomerate, with a consequent color change of AuNPs from wine red to purple or even blue. The kanamycin concentrations can be quantified by employing UV–vis spectrophotometer. The results display that kanamycin concentrations (0.005 to 18 µM) are linearly related to A<sub>620</sub>/A<sub>520</sub> (the absorbance ratio of 620 nm and 520 nm) with a LOD of 1.8 nM and a LOQ of 5.9 nM (S/N = 3). This strategy also reveals a high degree of selectivity among a series of common interfering species. Moreover, the strategy can be employed to detect trace amounts of kanamycin in real-life samples, and it shows satisfying results compared with high performance liquid chromatography. In general, this developed strategy is facile and inexpensive without the need for complex processing procedures and expensive instruments. In addition, this work may further exploit detection strategies for other organic contaminants, as well as make a strong contribution to the development of the colorimetric method.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cu doped ceria nanoparticles- rhodamine B as a novel chemiluminescence system and its application for nitrite detection in water and food samples 铜掺杂纳米二氧化铈-罗丹明B作为一种新型化学发光体系及其在水和食品中亚硝酸盐检测中的应用

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-14 DOI: 10.1007/s00604-024-06877-1

Niaz Jahed Tabrizi, Mohammad Amjadi, Tooba Hallaj

{"title":"Cu doped ceria nanoparticles- rhodamine B as a novel chemiluminescence system and its application for nitrite detection in water and food samples","authors":"Niaz Jahed Tabrizi, Mohammad Amjadi, Tooba Hallaj","doi":"10.1007/s00604-024-06877-1","DOIUrl":"10.1007/s00604-024-06877-1","url":null,"abstract":"<div><p>Fe, Ni, and Cu doped ceria nanoparticles (CeNPs) were prepared with a simple and one-pot hydrothermal synthesis method. We investigated the chemiluminescence (CL) interaction between these NPs and rhodamine B (Rh B) and found that the highest CL intensity was related to the Rh B- Cu doped CeNPs. We assigned that to the higher catalytic property of Cu doped NPs compared to the others. Cu doped CeNPs have been applied for the first time as a catalyst in chemiluminescence reactions. Rh B- Cu doped CeNPs reaction was introduced as a novel CL system, and its mechanism was studied. Considering the sensitivity, simplicity, portability, and rapidness of the CL methods, we applied the introduced reaction to the development of a CL sensor for nitrite monitoring. In the presence of nitrite, the CL intensity of the system decreased, and there was a linear relationship between the CL intensity and nitrite concentration in the range 0.5–100 µM. Based on this fact, a sensitive and selective CL sensor with the detection limit of 0.2 µM was established for nitrite detection in various food and water samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Amplification-free sensitive detection of Staphylococcus aureus by spherical nucleic acid triggered CRISPR/Cas12a and Poly T-Cu reporter 球形核酸触发CRISPR/Cas12a和Poly T-Cu报告基因无扩增灵敏检测金黄色葡萄球菌

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-14 DOI: 10.1007/s00604-024-06931-y

Xiaoyu Zhang, Ruimeng Sun, Haoran Zheng, Yanfei Qi

引用次数: 0

Electrochemical aptasensor based on zirconium/copper oxides embedded in mesoporous carbon derived from bimetallic metal–organic framework for ultrasensitive detection of miR-21 基于锆/铜氧化物包埋于双金属金属-有机框架衍生的介孔碳的电化学适体传感器，用于超灵敏检测miR-21

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-14 DOI: 10.1007/s00604-024-06925-w

Leila Gorgani, Maedeh Mohammadi, Ghasem Najafpour Darzi, Jahan Bakhsh Raoof

{"title":"Electrochemical aptasensor based on zirconium/copper oxides embedded in mesoporous carbon derived from bimetallic metal–organic framework for ultrasensitive detection of miR-21","authors":"Leila Gorgani, Maedeh Mohammadi, Ghasem Najafpour Darzi, Jahan Bakhsh Raoof","doi":"10.1007/s00604-024-06925-w","DOIUrl":"10.1007/s00604-024-06925-w","url":null,"abstract":"<div><p>A novel electrochemical aptasensor based on bimetallic zirconium and copper oxides embedded within mesoporous carbon (denoted as ZrO<sub>2</sub>CuO<sub>x</sub>@mC) was constructed to detect miRNA. The porous ZrO<sub>2</sub>CuO<sub>x</sub>@mC was created through the pyrolysis of bimetallic zirconium/copper-based metal–organic framework (ZrCu-MOF). The substantial surface area and high porosity of ZrO<sub>2</sub>CuO<sub>x</sub>@mC nanocomposite along with its robust affinity toward aptamer strands, facilitated the effective anchoring of aptamer strands on the ZrO<sub>2</sub>CuO<sub>x</sub>@mC-modified electrode surface. This, coupled with the remarkable electrochemical performance arising from the presence of metal oxides and mesoporous carbon, resulted in the exceptional sensitivity of the ZrO<sub>2</sub>CuO<sub>x</sub>@mC-based aptasensor for the detection of miR-21. The prepared aptasensor not only demonstrated a broad detection range from 10 zM to 100 pM, featuring an exceptionally low detection limit of 0.52 zM, but also exhibited notable selectivity against interferences. Moreover, it displayed good stability, reproducibility, and acceptable applicability for miR-21 detection in human serum. The fabricated aptasensor offers a promising platform for ultrasensitive miR-21 detection, with potential applications in accurate and early diagnosis of diseases related to miRNA.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trends in ready-to-use portable electrochemical sensing devices for healthcare diagnosis 医疗诊断即用型便携式电化学传感设备的发展趋势

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-14 DOI: 10.1007/s00604-024-06916-x

Anjana Sajeevan, Reshmi A. Sukumaran, Lakshmi R. Panicker, Yugender Goud Kotagiri

{"title":"Trends in ready-to-use portable electrochemical sensing devices for healthcare diagnosis","authors":"Anjana Sajeevan, Reshmi A. Sukumaran, Lakshmi R. Panicker, Yugender Goud Kotagiri","doi":"10.1007/s00604-024-06916-x","DOIUrl":"10.1007/s00604-024-06916-x","url":null,"abstract":"<div><p>Compared with previous decades, healthcare has emerged as a key global concern in light of the recurrent outbreak of pandemics. The initial stage in the provision of healthcare involves the process of diagnosis. Countries worldwide advocate for healthcare research due to its efficacy and capacity to assist diverse populations. Enhanced levels of healthcare management can be attained by the implementation of rapid diagnostic procedures and cognitive data analysis. Therefore, there is a constant need for smart therapeutics, analytical tools, and diagnostic systems to improve health and well-being. The past decade witnessed enormous growth in the sensing detection systems integrated into smartphones with printed electrodes and wearable patches for the screening of various healthcare diagnostics biomarkers and therapeutic drugs. This review focuses on the expansion of point-of-care technologies and their incorporation into a broader array of portable devices, a critical aspect in the context of decentralized societies and their healthcare systems. Discussions are broadly focused on the different sensing platforms such as solid electrodes, screen-printed electrodes, and paper-based sensing strategies for the detection of various biomarkers and therapeutic drugs. We also discuss the next-generation healthcare wearable sensing device importance and future research possibilities. Finally, the portable electrochemical sensing devices and their future perspective developments towards healthcare diagnosis are critically summarized.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly electroactive thiazolium [5,4-d]thiazol-2,5-dicarboxylic acid-silver electrochemiluminescent metal–organic frameworks: synthesis, properties and application in glutathione detection 高电活性噻唑[5,4-d]噻唑-2,5-二羧酸-银电化学发光金属-有机框架：合成、性质及其在谷胱甘肽检测中的应用

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-14 DOI: 10.1007/s00604-024-06948-3

Ru Yu, Haoran Zhang, Ying Tu, Fei Nie

{"title":"Highly electroactive thiazolium [5,4-d]thiazol-2,5-dicarboxylic acid-silver electrochemiluminescent metal–organic frameworks: synthesis, properties and application in glutathione detection","authors":"Ru Yu, Haoran Zhang, Ying Tu, Fei Nie","doi":"10.1007/s00604-024-06948-3","DOIUrl":"10.1007/s00604-024-06948-3","url":null,"abstract":"<div><p>Thiazolo[5,4-d]thiazole-2,5-dicarboxylic acid (H<sub>2</sub>Thz), a thiazolothiazole (TTz) derivative with carboxylic acid groups, was synthesized as a ligand for the creation of five MOFs, each associated with distinct metal ions including Ag<sup>+</sup>, Mn<sup>2+</sup>, Co<sup>2+</sup>, Zn<sup>2+</sup>, and Cu<sup>2+</sup>. The cathodic electrochemiluminescence (ECL) of H<sub>2</sub>Thz and the resulting MOFs was investigated. H<sub>2</sub>Thz was found to generate ECL signals, but this process was heavily reliant on potassium persulfate (K<sub>2</sub>S<sub>2</sub>O<sub>8</sub>) as a co-reactant. However, the Ag-MOF, formed with H<sub>2</sub>Thz as the ligand, exhibited substantial ECL signals without the need for any co-reactant, which is different from other metal-containing MOFs. The smaller band gap, higher electron mobility and the unique electrocatalytic activity of Ag<sup>+</sup> ions were confirmed to be the reasons for the excellent ECL performance of Ag-MOF. The interaction between thiol group of glutathione (GSH) and Ag-MOF suppressed the ECL signal of the Ag-MOF-K<sub>2</sub>S<sub>2</sub>O<sub>8</sub> system. Based on this, a sensitive ECL method for GSH was developed and effectively utilized for GSH quantification in cell lysates.</p><h3>Graphical Abstract</h3><p>The Ag-MOF, constructed from the distinctive electron-accepting properties of thiazolo[5,4-d]thiazole-2,5-dicarboxylic acid and the superior catalytic and conductive attributes of silver, was utilized as an elecetrochemiluminescence probe for the sensitive determination of glutathione in cell lysates</p>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Self-powered dual-photoelectrode photoelectrochemical aptasensor amplified by hemin/G-quadruplex-based DNAzyme hemin/ g -四联基DNAzyme扩增的自供电双光电电极光电感应传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-14 DOI: 10.1007/s00604-025-06958-9

Yibo Huang, Weishen Lu, Shengjun Che, Xiaoru Zhang, Xunyi Yuan

{"title":"Self-powered dual-photoelectrode photoelectrochemical aptasensor amplified by hemin/G-quadruplex-based DNAzyme","authors":"Yibo Huang, Weishen Lu, Shengjun Che, Xiaoru Zhang, Xunyi Yuan","doi":"10.1007/s00604-025-06958-9","DOIUrl":"10.1007/s00604-025-06958-9","url":null,"abstract":"<div><p>A self-powered dual-electrode aptasensor was developed for the detection of tumor marker carcinoembryonic antigen (CEA). The composite BiVO<sub>4</sub>/ZnIn<sub>2</sub>S<sub>4</sub>, which is capable of forming a Z-scheme heterojunction, was chosen as the photoanode, and the AuNP/CuBi<sub>2</sub>O<sub>4</sub> complex was chosen as the photocathode in photoelectrochemical (PEC) detection. The experiments showed that the constructed self-powered dual-electrode system had a good photoelectric response to white light, and the photocurrent signal of the photocathode was significantly enhanced under the influence of the photoanode. The introduction of G-quadruplex/hemin DNAzyme through aptamer recognition resulted in the generation of insoluble species through catalytic reaction, which led to a significant decrease in the photocurrent, and achieved a highly sensitive detection of the target CEA with a detection limit of 0.021 pg/mL. At the same time, the developed photocathode detection exhibited good selectivity and could effectively avoid interference, making it suitable for real sample analysis. It is expected that this study can facilitate the application of PEC sensors in point-of-care (POC) diagnosis for life analysis.</p><h3>Graphical Abstract</h3><p>Self-powered dual-photoelectrode photoelectrochemical aptasensor amplified by hemin/ G-quadruplex-based DNAzyme</p>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of an electrochemical sensor for the detection of methyl parathion with three-dimensional graphdiyne-carbon nanotubes 三维石墨烯-碳纳米管检测甲基对硫磷电化学传感器的构建

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-14 DOI: 10.1007/s00604-024-06934-9

Yuzhen Xue, Xiuxiu Wang, Baoyun Sun, Longgang Wang, Xihong Guo

{"title":"Construction of an electrochemical sensor for the detection of methyl parathion with three-dimensional graphdiyne-carbon nanotubes","authors":"Yuzhen Xue, Xiuxiu Wang, Baoyun Sun, Longgang Wang, Xihong Guo","doi":"10.1007/s00604-024-06934-9","DOIUrl":"10.1007/s00604-024-06934-9","url":null,"abstract":"<div><p>To enhance the application performance of graphdiyne (GDY) in electrochemical sensing, carbon nanotubes (CNTs) were grown in situ to construct three-dimensional nanoarchitectures of GDY-CNTs composites. GDY-CNTs showed superior electrochemical properties and detection response to MP when compared with GDY, as the in situ growth of CNTs significantly increased the electrode surface area and enhanced the electron transfer process. GDY-CNTs were successfully used to construct electrochemical sensors for methyl parathion (MP). The proposed sensor exhibited a wide linear relationship for MP ranging from 0.09 to 64.6 µM with a detection limit of 0.05 µM. Moreover, the sensor also showed good stability and acceptable reproducibility, which provided a feasible method for rapid and accurate detection of MP in real samples. This work provides an effective application of graphdiyne in electrochemical sensing with constructed three-dimensional GDY-CNTs.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A POCT assay based on commercial HCG strip for miRNA21 detection by integrating with RCA-HCR cascade amplification and CRISPR/Cas12a 结合RCA-HCR级联扩增和CRISPR/Cas12a，基于商用HCG条带的POCT检测miRNA21

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-14 DOI: 10.1007/s00604-024-06922-z

Yuan Li, Zhihui Zeng, Xuefei Lv, Hao Jiang, Anyi Li, Ying Liu, Yulin Deng, Xiaoqiong Li

{"title":"A POCT assay based on commercial HCG strip for miRNA21 detection by integrating with RCA-HCR cascade amplification and CRISPR/Cas12a","authors":"Yuan Li, Zhihui Zeng, Xuefei Lv, Hao Jiang, Anyi Li, Ying Liu, Yulin Deng, Xiaoqiong Li","doi":"10.1007/s00604-024-06922-z","DOIUrl":"10.1007/s00604-024-06922-z","url":null,"abstract":"<div><p> A point-of-care testing (POCT) assay based on commercial HCG strip was proposed for miRNA21 detection by integrating RCA-HCR cascaded isothermal amplification with CRISPR/Cas12a. Three modules were integrated in the proposed platform: target amplification module composed of rolling circle amplification (RCA) cascaded with hybridization chain reaction (HCR), signal transduction module composed of CRISPR/Cas12a combined with HCG-agarose gel beads probes, and signal readout module composed of commercial HCG strips. The proposed RCA-HCR-CRISPR/Cas12a-HCG strip assay for miRNA21 detection had high sensitivity, and the limit of detection was as low as 37 fM. The proposed assay showed excellent specificity for miRNA21, as other miRNAs did not caused interference for detection. The recoveries of miRNA21 were ranged from 89.0 to 118.0%. The intra-batch and inter-batch coefficient of variation (CV) were 10.1–13.4% and 11.9–14.5%, respectively, which indicated a high accuracy and precision, and the serum matrix did not cause any interference. With the advantages of low-cost, high sensitivity, visualization, and easy popularization, the proposed assay is expected to provide a powerful tool for early diagnosis of tumor disease miRNA, especially in resource-limited areas.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142976570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aptamer proximal enzyme cascade reactions for ultrafast detection of glucose in human blood serum 适体近端酶级联反应超快速检测人血清中葡萄糖。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2025-01-13 DOI: 10.1007/s00604-024-06935-8

Min Liu, Chuanlin Zhu, Zihe Dong, Zhangmin Wang, Huan Yang, Jie Li, Ke Li, Bo Shen, Xinmin Li, Ping Leng, Shijia Ding, Jinlin Guo, Juan Zhang

{"title":"Aptamer proximal enzyme cascade reactions for ultrafast detection of glucose in human blood serum","authors":"Min Liu, Chuanlin Zhu, Zihe Dong, Zhangmin Wang, Huan Yang, Jie Li, Ke Li, Bo Shen, Xinmin Li, Ping Leng, Shijia Ding, Jinlin Guo, Juan Zhang","doi":"10.1007/s00604-024-06935-8","DOIUrl":"10.1007/s00604-024-06935-8","url":null,"abstract":"<div><p> An innovative colorimetric sensing strategy was developed for the detection of glucose by the integration of glucose aptamer, glucose oxidase (GOx), and horseradish peroxidase (HRP), termed aptamer proximal enzyme cascade reactions (APECR). In the presence of glucose, aptamer binding enables GOx to catalyze glucose oxidation into H<sub>2</sub>O<sub>2</sub> efficiently. Subsequently, the adjacent HRP catalyzes the oxidation of the peroxidase substrate, 2,2′-biazobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS<sup>2−</sup>), utilizing the generated H<sub>2</sub>O<sub>2</sub>, resulting in a distinct color change. In comparison to the free enzymes and the HRP-GOx system, APECR exhibited higher colorimetric signal. This approach achieved glucose detection within three minutes, which was significantly faster than previous methods. This method showed good sensitivity and selectivity with a limit of detection of 0.013 mM. Moreover, the practical utility of this strategy was verified by achieving rapid detection of glucose in clinical serum samples. Hence, the developed strategy has the advantages of simple operation and rapid analysis time for the detection of glucose in human serum.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142969201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0