Microchimica Acta最新文献

{"title":"Rapid and home-testable integrated colorimetric sensor for nitrite detection in urine","authors":"Feng-Jiao Liu,&nbsp;Hua-Li Li,&nbsp; Ke-Shi,&nbsp;Shun-Xing Li,&nbsp;Jia-Yi Luo,&nbsp;Zhao-Jing Huang,&nbsp;Peiyuan Cai,&nbsp;Feng-Ying Zheng,&nbsp;Ling-Ling Su,&nbsp;Feng-Juan Han,&nbsp;Shang-qing Li,&nbsp; Fei-Liu,&nbsp;Hai-Li Lin","doi":"10.1007/s00604-026-08434-4","DOIUrl":"10.1007/s00604-026-08434-4","url":null,"abstract":"<div><p>A portable, rapid and convenient intelligent detection platform for nitrite is proposed based on a polyacrylonitrile NO₂⁻ colorimetric sensor (PAN-NP) integrated with a smartphone for household urinary nitrite detection. The PAN-NP membrane was prepared from polymer polyacrylonitrile (PAN) and polyvinylpyrrolidone (PVP) via electrospinning technology to protect the Griess reagent, resulting in prolonged stability for 30 days. Deep Convolutional Neural Network (DCNN) was utilized for color recognition to determine NO₂⁻ content, and it can detect NO₂⁻ in artificial urine over a concentration range 5.00-200.0 µM, with recoveries of 95.20–104.00% and accuracy of 99.50%. Compared to commercial test strip reflectance analysis, it lowers the detection limit for NO₂⁻ from 25.0 µM to 4.65 µM, facilitating earlier detection and treatment of urinary tract infections, and reducing the entire testing time to 2 min without complex preprocessing. This method has been successfully applied to detect nitrite in real urine samples, providing a reliable strategy for point-of-care testing of urinary nitrite and intelligent home health monitoring.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 11","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149098164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dual-readout smartphone-assisted and spectrophotometric colorimetric nanosensor for enzymeless bisphenol A detection","authors":"Aziz Balouch,&nbsp;Muhammad Imran,&nbsp;Tasmina Kanwal,&nbsp;Khadija Rehman,&nbsp;Muhammad Hasnain,&nbsp;Jafar Saifullah,&nbsp;Yue Li,&nbsp;Dong-Sheng Guo,&nbsp;Muhammad Raza Shah,&nbsp; Sirajuddin","doi":"10.1007/s00604-026-08443-3","DOIUrl":"10.1007/s00604-026-08443-3","url":null,"abstract":"<div><p>A proof-of-concept approach is introduced that uses silver nanoparticles modified with <i>Epimedium</i> plant extract (EE-AgNPs) for BPA detection. The EE-AgNPs aggregate in the presence of BPA, demonstrating a measurable color change. This color change is monitored via a smartphone, enabling the prompt on-site determination of BPA. The sensor exhibited a linear response within the range 0.1–50 µM and a limit of detection (LOD) of 22 nM, which is below the thresholds set by the United States Food and Drug Administration, the National Health and Family Planning Commission of China, and European Union’s specific migration limits, rendering it appropriate for the testing of polluted water and biological samples. Moreover, the study provides promising recoveries for BPA in water and biological specimens. The assimilation of the colorimetric system with smartphone offers a potential, cost-effective, and efficient option to traditional chromatographic approaches, especially for the initial evaluation of biological and polluted water samples.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 11","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149098594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zein–templated synthesis of Au@Pt bimetallic nanozymes: a multi–mode lateral flow immunoassay platform for ultrasensitive colorimetric and fluorometric detection of tetracycline in food","authors":"Anna Kerkula,&nbsp;Li Wang,&nbsp;Xiaohuan Gao,&nbsp;Lixin Xu,&nbsp;Wuyang Huang,&nbsp;Kun Zeng,&nbsp;Zhen Zhang","doi":"10.1007/s00604-026-08435-3","DOIUrl":"10.1007/s00604-026-08435-3","url":null,"abstract":"<div><p>The systematic development of a multifunctional zein–stabilized Au@Pt bimetallic nanozyme (zein–Au@Pt) for highly efficient multimodal detection of tetracycline (TC) is described. We created a core-shell nanostructure using zein protein as a biomimetic template, combining the Au@Pt bimetallic interface’s high peroxidase-mimicking activity with the prolamine shell’s fluorescence. Zein–Au@Pt exhibited low Michaelis constants (𝐾𝑚 for H₂O₂: 0.813 mM; TMB: 0.211 mM) in steady-state kinetics, indicating high substrate affinity. A multimodal lateral flow immunoassay (LFIA) platform allows traditional colorimetric screening, catalytically amplified detection, and fluorescence measurement. These three approaches had detection limits of 0.9, 0.09, and 0.014 ng/mL, respectively, demonstrating 10-fold (catalytic amplification) and 64-fold (fluorescence sensitization) increases in sensitivity over traditional colorimetric LFIA. High recoveries (89.8–109.3%) across various complex animal-derived matrices demonstrated its adaptability as a multimodal LFIA platform for on-site screening after minimal sample preprocessing for antibiotic residue surveillance. Despite these benefits, challenges remain, including potential cross-reactivity with TC analogs in complex matrices, the need to miniaturize the fluorescence readout device for true point-of-care use, and the long-term stability of the zein protein coating under different storage conditions. Engineering highly specialized recognition elements (aptamers) to overcome cross-reactivity, integrating smartphone-based detecting systems for mobility, and scaling up zein-templated nanozymes for commercial use are future priorities.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 11","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149098009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A dual-electropolymerized poly(Xylenol orange)@poly(Euchrysine) sensing interface for sustainable simultaneous electroanalysis of 5-fluorouracil and empagliflozin: density functional theory mechanistic elucidation","authors":"Rania S. Ibrahim,&nbsp;Hanaa M. Abd El-Wadood,&nbsp;Marwa R. El-Zahry,&nbsp;Antonio Frontera,&nbsp;Noha M. Hosny","doi":"10.1007/s00604-026-08402-y","DOIUrl":"10.1007/s00604-026-08402-y","url":null,"abstract":"<div><p>A sensitive and sustainable electroanalytical approach for the simultaneous determination of 5-fluorouracil (5-FU) and empagliflozin (EMPA) is desirable for pharmaceutical and bioanalytical investigations, particularly in preliminary studies exploring their concomitant use and potential protective effects. Herein, a square-wave voltammetric (SWV) method was developed using a sequentially fabricated poly-XO@poly-EUC/PGE sensing interface, prepared by successive electropolymerization of euchrysine (EUC) and xylenol orange (XO) on the surface of pencil graphite electrode (PGE). Electrochemical and morphological characterization confirmed successful electrode modification and improved interfacial electron-transfer properties. The sequential dual-polymer architecture produced a more pronounced analytical response than the corresponding individual polymer-modified electrodes, supporting a synergistic contribution of the two polymeric layers. Under optimized conditions, the proposed SWV method exhibited good linearity, precision (RSD ≤ 1.51%), and accuracy (recoveries of 96.21–103.59%), with detection limits of 0.33 and 0.02 µM for 5-FU and EMPA, respectively. The method also provided satisfactory recoveries in spiked human plasma, demonstrating its potential applicability to biological samples. An Environmental, Performance, and Practicality Index (EPPI) score of 78.0 highlighted the favorable sustainability and practical performance of the method. Density functional theory (DFT) calculations were performed using isolated EUC and XO molecular models and suggested favorable analyte–modifier interactions and provided qualitative molecular-level support for the proposed sensing mechanism, while the estimated oxidation pathway indicated a one-electron irreversible process. Overall, the poly-XO@poly-EUC/PGE platform provides a sensitive, selective, practical, and sustainable approach for the simultaneous electrochemical determination of 5-FU and EMPA, with potential utility in pharmaceutical quality control and bioanalytical studies.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 11","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-026-08402-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149098010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Density functional theory‑elucidated α-ZrP/phosphorus-nitrogen co-doped hollow carbon spheres composite for highly sensitive electrochemical detection of moxifloxacin","authors":"Yuan Jin,&nbsp;Yanhong Zhang,&nbsp;Jinxia Yao,&nbsp;Nanke Ma,&nbsp;Guangli Li","doi":"10.1007/s00604-026-08432-6","DOIUrl":"10.1007/s00604-026-08432-6","url":null,"abstract":"<div><p>Moxifloxacin (MOX) residues require reliable trace detection. Herein, an electrochemical sensor based on an α‑ZrP/phosphorus‑nitrogen co‑doped hollow carbon spheres (α‑ZrP/PNHC) composite is reported, where PNHC serves as a conductive scaffold to disperse α‑ZrP nanosheets and provide abundant transport pathways. Density functional theory (DFT) computations indicate that phosphorus atoms substitute into the carbon sites at the shoulder position next to pyridinic‑N, thereby injecting electrons into the carbon lattice and gently lowering the Lewis basicity of those pyridinic‑N centers. For the P‑doped pyridinic‑N site, the calculated adsorption energy amounts to − 0.745 eV, representing a moderate and ideal strength for stable enrichment without active site locking. Importantly, XPS data experimentally corroborate these findings, after P‑doping, the fraction of pyridinic‑N rises from 22.94% to 24.40%. This enrichment of pyridinic‑N, together with its moderate adsorption energy and open configuration, provides the ideal balance between stable enrichment and active site accessibility. Owing to these synergistic structural and electronic effects, the α‑ZrP/PNHC/GCE sensor delivers a broad linear detection interval (0.03–10 μM), a low limit of detection (0.023 μM), outstanding selectivity, acceptable reproducibility, and reliable stability. Recovery experiments in tap water and honey give values ranging from 97.29% to 103.70%, verifying the method’s practical utility. This work offers a highly sensitive sensing platform for MOX and provides DFT‑guided insights into the rational design of heteroatom‑doped carbon‑based composites.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 11","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149098011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A ratiometric nanozyme sensor with smartphone-assisted visual detection for rapid nitrite detection in meat products based on Mn2O3/MnNiO3 composite microspheres","authors":"Xuefang Zheng,&nbsp;Mengze Zhao,&nbsp;Qi Lian,&nbsp;Zhao’an Liu,&nbsp;Jiaqi Kang,&nbsp;Yingjie Du","doi":"10.1007/s00604-026-08424-6","DOIUrl":"10.1007/s00604-026-08424-6","url":null,"abstract":"<div><p>Rational design of high-performance nanozymes to replace natural enzymes remains a significant challenge in food safety monitoring. Herein, hierarchical Mn₂O₃/MnNiO₃ microspheres were developed via a facile solvothermal strategy for the sensitive detection of nitrite. The obtained dual-phase Mn₂O₃/MnNiO₃ composite exhibited favorable oxidase-like activity toward TMB oxidation. Structural characterization indicates that the optimized composite possesses a specific surface area of 16.29 m²/g and a mesoporous architecture. Mechanistic investigations using Electron Paramagnetic Resonance (EPR) and radical scavenging assays reveal that the introduced lattice defects promote the generation of superoxide anion radicals (·O₂⁻), which serve as the primary active species. The Mn₂O₃/MnNiO₃ microspheres showed oxidase-like activity, enabling rapid chromogenic signal generation for the subsequent assay. Leveraging this activity, a ratiometric colorimetric sensor was constructed by coupling the enzymatic oxidation of TMB with a nitrite-specific diazotization reaction. The assay demonstrates a linear range of 0-300 µM with an ultra-low detection limit (LOD) of 0.17 µM. Furthermore, a smartphone-assisted visual detection platform was developed, achieving an LOD of 2.5 µM based on the single B-channel response.</p><h3>Graphical Abstract</h3><p>Scheme 1. Schematic Overview of Mn2O3/MnNiO3 Microsphere-Based Ratiometric Colorimetric Detection of NO2-.</p><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microfluidic pretreatment devices for milk safety at the point of care","authors":"Ruihua Tang,&nbsp;Tingjuan Li,&nbsp;Haojie He,&nbsp;Keyu Chen,&nbsp;Feng Shi,&nbsp;Sufeng Zhang,&nbsp;Zedong Li","doi":"10.1007/s00604-026-08430-8","DOIUrl":"10.1007/s00604-026-08430-8","url":null,"abstract":"<div><p>Milk is a vital nutrient source, playing an essential role in maintaining normal physiological functions and safeguarding human health. However, milk consumption may expose individuals to residual contaminants such as antibiotics and environmental pollutants, which pose serious threats to human health. Currently, traditional milk pretreatment technologies hinder the rapid detection of milk residues due to their cumbersome procedures, operational complexity, prolonged processing time, and high dependence on specialized personnel. Microfluidic devices have emerged as a promising tool for food safety monitoring in resource-limited settings due to their simplicity, portability, and rapid processing capabilities. Nevertheless, the lack of systematic summaries on microfluidic device development for milk pretreatment limits their widespread application. To overcome these drawbacks, this review first presents the types and preparation methods of microfluidic devices employed in milk pretreatment. Subsequently, it summarizes the emerging applications of these devices in this field. Finally, the current challenges and prospects are discussed.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Copper-doped mesoporous silica nanoparticles for constructing a single-particle enzyme-nanozyme cascade sensing system","authors":"Yu Wu,&nbsp;Qing Liu,&nbsp;Pei-Lin Shen,&nbsp;Jia-Dong Zhang,&nbsp;Tian Shun Song,&nbsp;Xiang-Ling Li,&nbsp;Jing Jing Xie","doi":"10.1007/s00604-026-08438-0","DOIUrl":"10.1007/s00604-026-08438-0","url":null,"abstract":"<div><p>Copper-doped mesoporous silica nanoparticles (Cu-MSNs) were developed as multifunctional carriers to construct a single-particle enzyme-nanozyme cascade system for colorimetric glucose detection. The mesoporous framework provided abundant sites for glucose oxidase (GOx) immobilization and substrate diffusion, while the incorporated Cu⁺/Cu²⁺ species acted as catalytic active sites that endowed Cu-MSNs with peroxidase-like activity, enabling hydrogen peroxide (H₂O₂) activation and 3,3’,5,5’-tetramethylbenzidine (TMB) oxidation. GOx was immobilized onto amino-functionalized Cu-MSNs through electrostatic interactions to form GOx@Cu-MSNs. In this system, GOx catalyzed glucose oxidation to generate H₂O₂, which was then activated by Cu-MSNs to produce hydroxyl radicals and oxidize TMB into a blue product. The spatial integration of GOx and Cu catalytic sites within one particle facilitated intermediate utilization and enhanced cascade signal amplification compared to a physical mixture of free GOx and Cu-MSNs. After integration with a paper-based analytical device (PAD), the GOx@Cu-MSNs-modified PAD enabled smartphone-assisted glucose detection with a linear range of 20–400 µM and a detection limit of 13.6 µM. The PAD showed good selectivity, retained 95% of its initial response after 21 days of storage, and maintained the response in artificial and real sweat samples. This work provides a portable visual strategy with initial feasibility for sweat glucose analysis and demonstrates the potential of Cu-MSNs for building expandable enzyme-nanozyme cascade sensing platforms.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guanosine monophosphate-iron coordination nanozyme as a peroxidase mimetic for colorimetric detection of tetracycline antibiotics in food","authors":"Tingyue Yang,&nbsp;Jiazhen Shi,&nbsp;Yinghua Song,&nbsp;Xiaojuan Niu,&nbsp;Yuangen Wu,&nbsp;Zhaoyu Wang","doi":"10.1007/s00604-026-08436-2","DOIUrl":"10.1007/s00604-026-08436-2","url":null,"abstract":"<div><p>Residues of tetracycline antibiotics (TCs) in food pose a serious threat to human health, creating an urgent need for the development of simple, rapid, and highly sensitive detection methods. Herein, guanosine monophosphate-iron coordination nanozyme (Fe-GMP) were synthesized using the self-assembly of guanosine-5′-monophosphate and iron. Based on the peroxidase-mimicking activity of Fe-GMP nanozyme, a novel colorimetric sensor for the colorimetric detection of TCs was developed. Fe-GMP catalyzes the oxidation of chromogenic substrate 3,3′,5,5′-tetramethylbiphenylamine (TMB) in the presence of H₂O₂, producing a characteristic absorption peak at 652 nm. Interestingly, the introduction of TCs significantly enhances the peroxidase-like catalytic activity of Fe-GMP, causing the solution color to deepen from light to dark blue. Under optimized experimental conditions, the sensor exhibited a linear detection range of 0.2 µmol·L^− 1 to 6 µmol·L^− 1 for tetracycline, with a limit of detection (LOD) as low as 57.9 nmol·L^− 1 (26.7 µg·kg^− 1). The sensor has been successfully applied to determine tetracycline antibiotics in real milk and honey samples, with spiked recoveries ranging from 90% to 94%. The developed sensor exhibits a typical “signal-on” response mode, effectively avoiding the false-positive issues caused by nonspecific inhibition in traditional “signal-off” sensors. Furthermore, the sensor is cost-effective, highly efficient, and easy to operate, demonstrating great potential for rapid screening in food safety.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serum-compatible aptamer-regulated RCA–CRISPR/Cas12a assay for fluorescence and lateral flow detection of myeloperoxidase","authors":"Yingying Cao,&nbsp;Mengchen Duan,&nbsp;Lizhen Wei,&nbsp;Yisheng Chen,&nbsp;Fei Liu,&nbsp;Jiawen Bai,&nbsp;Yi Huang,&nbsp;Shenghang Zhang","doi":"10.1007/s00604-026-08427-3","DOIUrl":"10.1007/s00604-026-08427-3","url":null,"abstract":"<div><p>Myeloperoxidase (MPO), a neutrophil-derived heme peroxidase involved in oxidative inflammatory responses, has been investigated as a biomarker for cardiovascular, hematological, and other inflammation-associated diseases. In this study, an aptamer-regulated rolling circle amplification (RCA)–CRISPR/Cas12a fluorescence assay was established for MPO detection in serum samples. Two aptamers, Apt-02 and Apt-14, were obtained with dissociation constants (<i>K</i>_<i>D</i>) of 57.76 and 56.88 nM, respectively, using MPO-depleted human serum as the counter-selection matrix during MB-SELEX. Apt-14 exhibited relatively good stability in serum and was therefore used in the assay development. Upon MPO binding, cDNA was released from the aptamer/cDNA duplex, initiating RCA and subsequently activating Cas12a-mediated trans-cleavage to amplify the fluorescence signal. Under the optimized conditions, the assay achieved a detection limit of 0.422 ng/mL with good selectivity. It also showed limited interference from the tested cardiovascular disease-related proteins and provided MPO levels in clinical serum samples that were consistent with ELISA results. The assay was also adapted to a lateral-flow strip using a biotin/FAM-labeled ssDNA reporter, enabling visual detection and reader-assisted semi-quantitative analysis.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}