Microchimica Acta最新文献

{"title":"Antenna effect on Zn(II) porphyrin–based molecular ensembles for the detection of 2,4-dinitrophenol through energy and electron transfer process","authors":"Prasanth Palanisamy,&nbsp;Mageshwari Anandan,&nbsp;Gurusamy Raman,&nbsp;Venkatramaiah Nutalapati","doi":"10.1007/s00604-024-06795-2","DOIUrl":"10.1007/s00604-024-06795-2","url":null,"abstract":"<div><p>Two modular systems were synthesized composed of triphenylamine (ZnTPAP) and pyrene (ZnPyP) covalently linked at meso position of the Zn(II) porphyrins. Both compounds behaved as energy transfer antenna and orthogonal units to enhance the electron donating ability of Zn(II) porphyrins. Detailed photophysical and aggregation studies reveal that an appreciable electronic interaction exists between peripheral units to the porphyrin <i>π</i>-system so that they behave like strong donor materials. The electrochemical and computational studies demonstrate delocalization of the frontier highest occupied molecular orbital (−5.08 eV) over the triphenylamine entities (ZnTPAP) in addition to the porphyrin macrocycle. Fluorescence experiments with ZnTPAP and ZnPyP in the presence of different nitro analytes at various concentrations show <i>turn-off</i> fluorescence behaviour and exhibit superior selectivity towards 2,4-dinitrophenol (DNP) with limit of detection (LOD) of ~ 2.3 and 9.2 ppm for ZnTPAP and ZnPyP. Photoinduced electron transfer process is involved in the static and dynamic fluorescence quenching process. A Stern–Volmer quenching association constant (K_sv) determination revealed that ZnTPAP is more sensitive than the ZnPyP. This is attributed to the strong donating behaviour of TPA units caused by intermolecular interaction through metal center and strong <i>π</i>–<i>π</i> interactions with nitro analytes. The present study provides new insights into the ability to tune the affinity and selectivity of porphyrin-based sensors utilising electronic factors associated with the central Zn(II) ion. Furthermore, a smartphone-interfaced portable fluorimetric method by recognising colour variations in RGB and the luminance (L) values facilitate sensitive and real-time sensing at low concentration levels will have a significant impact on development of a new class of chemosensors.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142758001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progress of black phosphorene from preparation to diversified bio-/chemo-nanosensors and their challenges and opportunities for comprehensive health","authors":"Ting Xue,&nbsp;Xinyu Lu,&nbsp;Yangping Wen,&nbsp;Hassan Karimi Maleh,&nbsp;Xuemin Duan,&nbsp;Jingkun Xu","doi":"10.1007/s00604-024-06828-w","DOIUrl":"10.1007/s00604-024-06828-w","url":null,"abstract":"<div><p>The introduction of comprehensive health, related to human living environment and mental state, helps people to improve human health literacy and accept scientific health guidance. The unique structure and properties of black phosphorene (BP) provide potential opportunities for rapid development and versatile applications of high-performance sensors serving comprehensive health. The review begins with the preparation from bulk black phosphorous crystals via transforming requirements of phosphorous allotropes and BP nanosheets via preparative strategies using both “top-down” and “bottom-up” methods. Then the diversified modification of BP and versatile fabrication of diversified bio-/chemo-nanosensors for sensitive detection of analytes are discussed. Besides, the challenges including the preparation of BP, diversified modification, devices for improving performance defects and chemo-/bio-nanosensors for enhancing performance are outlined together with potential opportunities for the BP preparation and applications in comprehensive health from agricultural environments, food safety, personal life, physical and mental life, and finally to medical care.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A facile synthesis of polyethyleneimine based fluorescent polymeric nanorods using vitamin B6 cofactor as cross-linker for ratiometric detection of doxorubicin","authors":"Anuj K Saini,&nbsp;Ritambhara Jangir,&nbsp;Bigyan Ranjan Jali,&nbsp;Suban K Sahoo","doi":"10.1007/s00604-024-06856-6","DOIUrl":"10.1007/s00604-024-06856-6","url":null,"abstract":"<div><p>Polyethyleneimine (PEI) based fluorescent polymeric nanorods with high quantum yield (87.21%) were synthesized by crosslinking and self-assembly of PEI by vitamin B₆ cofactor pyridoxal 5’-phosphate (PLP). The fluorescent PEIPLP polymeric nanorods were employed for the ratiometric detection of doxorubicin (DOX). With the addition of DOX to the PEIPLP nanorods, the fluorescent intensity was decreased at 510 nm and concomitantly enhanced at 590 nm due to the fluorescence resonance energy transfer (FRET) process. The selective and specific ratiometric fluorescence response from the PEIPLP nanorods can be employed to detect DOX down to 10 nM. The practical utility of the PEIPLP nanorods was examined by detecting DOX in human serum.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of a visual assessment platform for total antioxidant capacity of Ganoderma sichuanense based on Arg-CeO2 hydrogel nanozyme utilizing a self-programmed smartphone app and a self-designed 3D-printed device","authors":"Suhui Tan,&nbsp;Mengyuan Tan,&nbsp;Tao Zhang,&nbsp;Lu Gao,&nbsp;Yi Li,&nbsp;Xuechao Xu,&nbsp;Zhen-quan Yang","doi":"10.1007/s00604-024-06840-0","DOIUrl":"10.1007/s00604-024-06840-0","url":null,"abstract":"<div><p> A multienzyme-like L-argininemodified CeO₂ (Arg-CeO₂) nanozyme was prepared for accurate total antioxidant capacity (TAC) determination of <i>Ganoderma sichuanense </i>(<i>G. sichuanense</i>). It exhibits oxidase-like and peroxidase-like activities catalyzing the generation of superoxide anion free radicals and hydroxyl radicals, accelerating 3, 3′, 5, 5′-tetramethylbenzidine (TMB) chromogenic reaction. Antioxidants can effectively eliminate the generated free radicals, inhibiting the TMB chromogenic reaction. Hence, TAC can be assessed by Arg-CeO₂ catalyzed TMB chromogenic reaction using ascorbic acid (AA) as a reference substance. TAC of <i>G. sichuanense </i>extracted using different solvents was determined, indicating the highest TAC observed in the <i>G. sichuanense </i>extracted by the mixture solvent (deionized water/ethanol/ethyl acetate = 1 : 1 : 1). A visual assessment platform based on Arg-CeO₂ hydrogel nanozyme utilizing a self-programmed smartphone app and a self-designed 3D printed device is established. TAC using AA as a reference substance (3.33 ~ 83.33 µM) can be detected with a detection limit as low as 0.58 µM. The platform can realize one-step quantitative determination of TAC in <i>G. sichuanens</i>e within 40 min. The proposed visual assessment platform presented exhibits promising application prospects in the field of TAC assessment in food.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Highly sensitive voltammetric determination of the fungicide fenhexamid using a cost-effective and disposable pencil graphite electrode","authors":"Teslime Erşan,&nbsp;Didem Giray Dilgin,&nbsp;Ayhan Oral,&nbsp;Sławomira Skrzypek,&nbsp;Mariola Brycht,&nbsp;Yusuf Dilgin","doi":"10.1007/s00604-024-06804-4","DOIUrl":"10.1007/s00604-024-06804-4","url":null,"abstract":"<div><p> A differential pulse voltammetric (DPV) method is proposed for the highly sensitive determination of fenhexamid (FHX) based on both electrooxidation and electroreduction processes using a disposable and cost-effective pencil graphite electrode (PGE). The electrochemical oxidation and reduction mechanisms of FHX at the PGE were elucidated by recording cyclic voltammograms at various pH values of Britton–Robinson buffer (BRB) solutions at a scan rate of 50 mV s⁻¹ and different scan rate values in the range 10–400 mV s⁻¹ at selected pH of BRB (pH 2.0). Differential pulse voltammograms recorded under optimized conditions revealed an oxidation peak of FHX around + 0.65 V and a reduction peak of FHX around + 0.45 V. The DPV analysis of FHX revealed two linear ranges: 0.001–0.01 µmol L⁻¹ and 0.01–5.0 µmol L⁻¹ for the anodic peak, and 0.001–0.1 µmol L⁻¹ and 0.1–5.0 µmol L⁻¹ for the cathodic peak. The limits of detection were 0.34 nmol L⁻¹ and 0.32 nmol L⁻¹ for the anodic and cathodic peaks, respectively. The proposed methodology demonstrated satisfactory selectivity, as selected pesticides, certain electroactive compounds, and cationic species tested did not interfere with the voltammetric determination of FHX, particularly during its reduction. The recovery results, showing values close to 100% obtained from the analysis of real samples spiked with FHX, indicated that this methodology can accurately determine FHX in water and soil samples.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06804-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel unlabeled electrochemical sensing platform based on highly electroactive Cu-MOF film for nanoplastic detection in water","authors":"Xue Wang,&nbsp;Zixuan Zhao,&nbsp;Long An,&nbsp;Tianxiang Wang,&nbsp;Xiaojing Yang,&nbsp;Jiajia Shan","doi":"10.1007/s00604-024-06841-z","DOIUrl":"10.1007/s00604-024-06841-z","url":null,"abstract":"<div><p>An unlabeled electrochemical sensing strategy based on electroactive copper-centered metal–organic framework (Cu-MOF) film coupled with multiwalled carbon nanotubes (MWCNTs) was proposed for the rapid assessment of nanoplastic concentration. The sensing interface was fabricated via the electro-deposition of Cu-MOF on the pre-modified MWCNTs using the cathodic reduction method. The exposed copper active sites in Cu-MOF showed excellent electrochemical activity, which was further enhanced due to rapid electron transfer induced by highly conductive MWCNTs. Through the adsorption functionality of Cu-MOF film towards polystyrene (PS) nanoplastics, the rapid recognition for nanoplastics in aqueous solution was achieved, thereby causing the inhibition of the current response. The results showed a robust dependence of the inhibition rate on the PS mass concentration. The proposed detection method was used for the quantitative determination of PS nanoplastics with the sizes of 100 nm, 500 nm, and 1 μm. The applicability of this electrochemical sensing platform was successfully validated in real-world water sample analysis.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dummy-template imprinted bovine serum albumin for extraction of zearalenone","authors":"Kirill Yu. Presnyakov,&nbsp;Polina M. Ilicheva,&nbsp;Daria V. Tsyupka,&nbsp;Ekaterina A. Khudina,&nbsp;Mikhail V. Pozharov,&nbsp;Pavel S. Pidenko,&nbsp;Natalia A. Burmistrova","doi":"10.1007/s00604-024-06790-7","DOIUrl":"10.1007/s00604-024-06790-7","url":null,"abstract":"<p>The aim of this study is to develop molecularly imprinted protein specific to zearalenone (ZEN). The primary idea of our study was to replace the toxic template—ZEN—with a dummy-template—4–hydroxicoumarin—during the synthesis of imprinted proteins (IPs). The choice of the dummy-template was based on the results of comprehensive evaluation that included a combination of blind docking and molecular dynamics simulations. Furthermore, we studied the influence of protonation and purification conditions to IPs sorption capacity. 3D fluorescence spectroscopy was used to monitor the process of bovine serum albumin (BSA) imprinting. The modified purification approach allowed reducing the eluent volume and purification time by approximately 300 and 48 times, respectively. The imprinted BSA was then used to produce a bioinorganic sorbent (IPs–BIS) based on silica nanoparticles (silica NPs), that, as far as we know, was never described before. The synthesized IPs–BIS were successfully applied as ZEN sorbents in model solutions (Q<span>(_text {max})</span> = 1.70 ± 0.15 mg g<span>(^{-1})</span>, imprinting factor = 2.5) and artificially contaminated wheat extract (Q<span>(_text {max})</span> = 2.24 ± 0.02 mg g<span>(^{-1})</span>) confirmed by HPLC–UV. We believe that our method can be used for mycotoxin monitoring in animal feeds and foodstuff.</p><p>Dummy-template imprinted bovine serum albumin for extraction of zearalenone</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142736817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a green-synthesized molecularly imprinted polymer-based electrochemical nanosensor for the determination of N-nitrosodimethylamine (NDMA) in serum and tap water","authors":"Nimisha Jadon,&nbsp;Ahmet Cetinkaya,&nbsp;Goksu Ozcelikay-Akyildiz,&nbsp;S. Irem Kaya,&nbsp;Esen Bellur Atici,&nbsp;Lokman Uzun,&nbsp;Sibel A. Ozkan","doi":"10.1007/s00604-024-06850-y","DOIUrl":"10.1007/s00604-024-06850-y","url":null,"abstract":"<div><p>N-nitrosodimethylamine (NDMA) was determined using a molecularly imprinted polymer (MIP)-based electrochemical sensor. Green-synthesized silver nanoparticles were functionalized with cysteamine to enhance their integration into the electrode surface, which was used to modify a glassy carbon electrode (GCE). Furthermore, a MIP-based electrochemical sensor was constructed via electropolymerization of 3-aminophenyl boronic acid (3-APBA) as a conjugated functional monomer in the presence of lithium perchlorate (LiClO₄) solution as a dopant, chitosan as a carrier natural polymer, and NDMA as a template/target molecule. The polymer film was characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The analytical performance of the silver nanomaterial-based MIP-based electrochemical (AgNPs@Chitosan/3-APBA@MIP-GCE) sensor was evaluated under optimized conditions. The linear range of NDMA was 1.0 × 10^–13–1.0 × 10^–12 M (0.1–1.0 pM), with a limit of detection (LOD) of 3.63 × 10^–15 M (3.63 fM) using differential pulse voltammetry (DPV). Method validation figured out that the developed MIP-based electrochemical nanosensor exhibited excellent selectivity, accuracy, and precision, which was shown by the analysis of synthetic serum samples and tap water. The LOD and LOQ in serum samples were 17.8 fM and 59.5 fM, respectively, which were in agreement with the developed method. Good recovery results confirm the successful application of the method in serum and tap water samples. The selectivity of the developed AgNPs@Chitosan/3-APBA@MIP-GCE sensor for NDMA was demonstrated in the presence of NDEA, sartans (valsartan, losartan, irbesartan, candesartan, telmisartan), and potential interferents that are possibly present in biological fluids (dopamine, ascorbic acid, uric acid) besides ionic species (sodium, chloride, potassium, nitrate, magnesium, sulfate) and common analgesic paracetamol.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142736937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature-dependent luminescent copper nanoclusters with noncovalent interactions for determination of β-galactosidase activity","authors":"Yanping Chang,&nbsp;Jingyi Wang,&nbsp;Hongwei Guo,&nbsp;Wanqing Yao,&nbsp;Haijiao Xie,&nbsp;Long Li,&nbsp;Xianhu Liu","doi":"10.1007/s00604-024-06844-w","DOIUrl":"10.1007/s00604-024-06844-w","url":null,"abstract":"<div><p>The synthesis of a novel bidentate ligand-protected copper nanocluster via a solid-state strategy is reported. Single-crystal X-ray diffraction analysis result reveals that the copper nanocluster features an octahedral core (Cu₆) coordinated by six ligands. Noncovalent interactions (C-H^<b>…</b>π and π^<b>…</b>π) exist between the copper nanoclusters. The copper nanocluster displays luminescence even at 250 °C. The luminescence intensity is linearly correlated with temperature changes. The copper nanocluster can assemble into luminescent nanosheets whose emission is quenched by 4-nitrophenol. Spectroscopic analysis and theoretical calculations results demonstrate that the inner filter effect and electron transfer cause the above quenching effect. A probe based on luminescent nanosheets was constructed for β-galactosidase activity determination. The linearity range is 3.3–91.8 U·L⁻¹, and the limit of detection is 0.45 U·L⁻¹. This probe was also evaluated for determination of the β-galactosidase activity in human serum via spiking experiments. The recoveries ranged from 96.2% to 101.8%.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142736816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic poly(phages) encoded probes–based dual-mode assay for rapid determination of live Escherichia coli and Hafnia paralvei based on microfluidic chip and ATP bioluminescence meter","authors":"Ye Wang,&nbsp;Ming Wang,&nbsp;Hang You,&nbsp;Chao Chen,&nbsp;Jing Zhang,&nbsp;Tianhua Li,&nbsp;Ning Gan","doi":"10.1007/s00604-024-06809-z","DOIUrl":"10.1007/s00604-024-06809-z","url":null,"abstract":"<div><p> A dual-mode assay was developed for screening and detecting live <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Hafnia paralvei</i> (<i>H. paralvei</i>) (as two typical pathogens in aquatic environments) based on magnetic poly(phages) encoded probes (MPEP). The probes were prepared by grafting a large number of phages targeting different target bacteria on a long-chain DNA structure, respectively. They could specifically capture and enrich <i>E. coli</i> and <i>H. paralvei</i> by magnetic separation. Then, different DNA signal tags with different lengths conjugate with the corresponding MPEP-bacteria complex and form two kinds of sandwich structures, respectively. After that, the captured <i>E. coli</i> and <i>H. paralvei</i> were lysed to release both adenosine triphosphate (ATP) and DNA signal tags. The measurement includes two steps. Firstly, a portable ATP bioluminescence meter was employed to rapidly screen the positive samples that contain either of the two target bacteria. Secondly, only positive samples were injected into the microfluidic chip which could detect various DNA signal tags for accurate quantification of the target bacteria. The assay demonstrated high sensitivity (3 CFU/mL for <i>E. coli</i> and 5 CFU/mL for <i>H. paralvei</i>), high specificity (strain identification), signal amplification (20-fold), and short time (≤ 35 min). It can be applied to detect other pathogens solely by changing the relative phage in MPEP. Furthermore, the proposed dual-mode assay provides a wide prospect for rapid screening and accurate determination of live foodborne pathogens. Clinical Trial Number: nbdxms-20240322.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06809-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}