CeO2/g-C3N4 photoactive nanozyme-based colorimetric immunoassay for carcinoembryonic antigen detection

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-09-25 DOI:10.1007/s00604-025-07524-z

Yan Cheng, Yunzhi Wang, Pengcheng Zhang, Xing Hu, Xiaolin Hou, Bing Zhang

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引用次数: 0

Abstract

Enzyme-mimicking nanomaterials have gained significant attention for use in colorimetric immunoassays. However, further enhancing their mimetic enzyme activity remains crucial for improving assay sensitivity. In this study, CeO₂/g-C₃N₄ nanozymes were synthesized with enhanced catalytic activity when exposed to visible light. The irradiation facilitates electron transfer within the CeO₂/g-C₃N₄ nanomaterials, leading to the generation of reactive oxygen species (ROS). These ROS then promote the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB), causing a distinct color change. Based on this, a novel colorimetric immunoassay platform was developed, using the CeO₂/g-C₃N₄ nanomaterials as labels. Under visible light, the color development signal was significant amplified, resulting in improved sensitivity. This approach showed a linear response for the detection of carcinoembryonic antigen (CEA) in the range 0.5–30 ng/mL, with a limit of detection (LOD) of 0.13 ng/mL. The assay also demonstrated high specificity and excellent reproducibility. Consequently, this method offers a promising strategy for early cancer diagnosis and holds considerable potential for wider clinical applications.

Graphical Abstract

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基于CeO2/g-C3N4光活性纳米酶的比色免疫法检测癌胚抗原。

酶模拟纳米材料在比色免疫测定中得到了极大的关注。然而，进一步提高它们的模拟酶活性仍然是提高分析灵敏度的关键。在本研究中，CeO2/g-C3N4纳米酶在可见光下的催化活性增强。辐照促进了CeO2/g-C3N4纳米材料内部的电子转移，导致活性氧（ROS）的产生。然后，这些ROS促进3,3',5,5'-四甲基联苯胺（TMB）的氧化，导致明显的颜色变化。在此基础上，建立了以CeO2/g-C3N4纳米材料为标记的新型比色免疫分析平台。在可见光下，显色信号被显著放大，从而提高了灵敏度。该方法对癌胚抗原（CEA）的检测在0.5 ~ 30 ng/mL范围内呈线性响应，检出限（LOD）为0.13 ng/mL。该方法具有高特异性和良好的重现性。因此，该方法为早期癌症诊断提供了一种有希望的策略，并具有广泛的临床应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.