Lejing Yao, Xiaoming Sun, Qing Tang, Jingwei Qiu, Da Liu, Cheng-Yu Li
{"title":"生物熵驱动的催化交联超灵敏纳米生物传感器，用于肿瘤生物标志物的精确近红外光调节成像。","authors":"Lejing Yao, Xiaoming Sun, Qing Tang, Jingwei Qiu, Da Liu, Cheng-Yu Li","doi":"10.1007/s00604-025-07554-7","DOIUrl":null,"url":null,"abstract":"<div><p>Owing to the protease-free and hairpin DNA primers-free design of entropy-powered catalysis (EPC), nanobiosensors constructed from this amplification protocol show promising potential for detecting tumor biomarkers in live biosystems. Nevertheless, EPC’s single-round signal enhancement and perpetual activation during bio-delivery severally restrict its sensitivity and precision in responding to low-concentration analytes. To overcome these restrictions, this study first links together fuel strands produced by two separate EPC modules, thus establishing an ultrasensitive nanobiosensor that implements an exceptional crosslinked bientropy-powered catalysis with more powerful two-round amplification capacity. Following that, one DNA segment is embedded with a photocleavable bond to block the analyte’s recognition site, whereby a near-infrared light (NIR)-regulated strategy facilitated by upconversion luminescence is utilized to precisely activate the biosensing operation. Our conceptual proof is validated by determining microRNA-21, a low-abundance biomarker inclined to be overexpressed in various malignant tumors. In addition to ultra-high sensitivity (with a remarkably low limit of detection of 32.44 fM) and satisfactory specificity (discriminating even single-nucleotide mutations) in buffered environments, this bientropy-powered catalysis crosslinked nanobiosensor enables reliable imaging assays in both live cells and animals, offering a potent toolbox for cancer diagnostics.</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Bientropy-powered catalysis crosslinked ultrasensitive nanobiosensor for precise NIR light-regulated imaging of tumor biomarkers\",\"authors\":\"Lejing Yao, Xiaoming Sun, Qing Tang, Jingwei Qiu, Da Liu, Cheng-Yu Li\",\"doi\":\"10.1007/s00604-025-07554-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Owing to the protease-free and hairpin DNA primers-free design of entropy-powered catalysis (EPC), nanobiosensors constructed from this amplification protocol show promising potential for detecting tumor biomarkers in live biosystems. Nevertheless, EPC’s single-round signal enhancement and perpetual activation during bio-delivery severally restrict its sensitivity and precision in responding to low-concentration analytes. To overcome these restrictions, this study first links together fuel strands produced by two separate EPC modules, thus establishing an ultrasensitive nanobiosensor that implements an exceptional crosslinked bientropy-powered catalysis with more powerful two-round amplification capacity. Following that, one DNA segment is embedded with a photocleavable bond to block the analyte’s recognition site, whereby a near-infrared light (NIR)-regulated strategy facilitated by upconversion luminescence is utilized to precisely activate the biosensing operation. Our conceptual proof is validated by determining microRNA-21, a low-abundance biomarker inclined to be overexpressed in various malignant tumors. In addition to ultra-high sensitivity (with a remarkably low limit of detection of 32.44 fM) and satisfactory specificity (discriminating even single-nucleotide mutations) in buffered environments, this bientropy-powered catalysis crosslinked nanobiosensor enables reliable imaging assays in both live cells and animals, offering a potent toolbox for cancer diagnostics.</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 10\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-09-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-025-07554-7\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07554-7","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Bientropy-powered catalysis crosslinked ultrasensitive nanobiosensor for precise NIR light-regulated imaging of tumor biomarkers
Owing to the protease-free and hairpin DNA primers-free design of entropy-powered catalysis (EPC), nanobiosensors constructed from this amplification protocol show promising potential for detecting tumor biomarkers in live biosystems. Nevertheless, EPC’s single-round signal enhancement and perpetual activation during bio-delivery severally restrict its sensitivity and precision in responding to low-concentration analytes. To overcome these restrictions, this study first links together fuel strands produced by two separate EPC modules, thus establishing an ultrasensitive nanobiosensor that implements an exceptional crosslinked bientropy-powered catalysis with more powerful two-round amplification capacity. Following that, one DNA segment is embedded with a photocleavable bond to block the analyte’s recognition site, whereby a near-infrared light (NIR)-regulated strategy facilitated by upconversion luminescence is utilized to precisely activate the biosensing operation. Our conceptual proof is validated by determining microRNA-21, a low-abundance biomarker inclined to be overexpressed in various malignant tumors. In addition to ultra-high sensitivity (with a remarkably low limit of detection of 32.44 fM) and satisfactory specificity (discriminating even single-nucleotide mutations) in buffered environments, this bientropy-powered catalysis crosslinked nanobiosensor enables reliable imaging assays in both live cells and animals, offering a potent toolbox for cancer diagnostics.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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