Microchimica Acta最新文献

{"title":"Covalent Michael-type recognition-based dual-mode sensing platform using thiol-functionalized nitrogen-doped carbon dots and Fe-MOF nanozyme for ultrasensitive patulin detection","authors":"Ashraf M Mahmoud,&nbsp;Ali O Alqarni,&nbsp;Rayed Ali A. Alqahtani,&nbsp;Yahya S. Alqahtani,&nbsp;Bandar A. Alyami,&nbsp;Sameera Sh. Mohammed Ameen,&nbsp;Khalid M. Omer,&nbsp;Mohamed M. El-Wekil,&nbsp;Ramadan Ali,&nbsp;Al-Montaser Bellah H. Ali","doi":"10.1007/s00604-026-08420-w","DOIUrl":"10.1007/s00604-026-08420-w","url":null,"abstract":"<div><p>Patulin is a toxic mycotoxin commonly found in apple and fruit products, requiring sensitive and reliable detection methods. Herein, a novel dual-mode sensing platform integrating turn-off fluorescence and colorimetric detection was developed for rapid patulin determination in apple juice. The strategy exploits patulin’s unique electrophilic reactivity toward thiols across two independent signal channels. Thiol-functionalized nitrogen-doped carbon dots (SH-N-CDs) serve as both recognition element and signal transducer, where direct covalent Michael-type addition of patulin onto the CD surface induces static fluorescence quenching confirmed by time-resolved lifetime measurements and XPS analysis. Simultaneously, patulin consumes free cysteine through PAT–cysteine adduct formation, relieving cysteine-mediated inhibition of Fe-MOF nanozyme peroxidase-like activity and restoring TMB oxidation to generate a proportional colorimetric signal. Under optimized conditions, the fluorometric and colorimetric methods exhibited linear ranges of 0.1–50.0 and 5.0–50.0 µg/L with limits of detection of 0.026 and 2.11 µg/L, respectively. Satisfactory recoveries of 97.0–100.4% with RSDs below 3.8% were obtained in spiked apple juice samples, with results showing no statistically significant difference from reference HPLC analysis. Successful determination of patulin in ten commercial apple juice samples further confirms the practical applicability of the proposed platform for routine food safety monitoring.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pair of inverse ratiometric sensing paradigms based on stimulus response of AIE@ZIFs via solvent-assisted aggregation-induced emission modulation and substituent-dependent ligand emission regulation for dynamic monitoring of PFAS-induced ATP changes in cardiomyocytes","authors":"Yiwen Min,&nbsp;Ziyang Ge,&nbsp;Chunqian Yu,&nbsp;Shu Zhang,&nbsp;Chenyi Xue,&nbsp;Ying Zhang,&nbsp;Tianshu Zhou,&nbsp;Wanying Zhai,&nbsp;Jingjing Deng","doi":"10.1007/s00604-026-08433-5","DOIUrl":"10.1007/s00604-026-08433-5","url":null,"abstract":"<div><p>Two aggregation-induced emission (AIE) guests, tetra[4-(3,5-dicarboxyphenyl)] tetraphenylethylene (H₈ETTB) and dispersed Cu nanoclusters (D-CuNCs), were separately encapsulated into ZIF-8 and ZIF-90, which bear methyl-substituted and aldehyde-functionalized imidazole ligands, respectively. The constructed AIE@ZIFs (H₈ETTB@ZIF-8 and A-CuNCs@ZIF-90) integrate dual emissions from the guests and the intrinsic ligand emission (LE) of the ZIF skeletons. Based on ATP-triggered responses, we developed inverse ratiometric sensing paradigms through solvent-assisted AIE modulation and substituent-dependent LE regulation, which have been rarely explored. The distinct fluorescence color changes (bluish-green to green; orange-red to blue), arising from opposite emission responses of the guests and ligands, enable reliable color-oriented analysis. H₈ETTB@ZIF-8 affords a linear range of 0.02-6 mM ATP with detection limits of 9.84 µM (spectral) and 19.86 µM (smartphone), while A-CuNCs@ZIF-90 exhibits a linear range of 0.01-3 mM with limits of 4.26 µM and 8.52 µM, respectively. Both nanoprobes show outstanding anti-interference performance and can be internalized into cardiomyocytes (H9c2) for in situ imaging of ATP dynamics and accurate quantification of PFAS-induced ATP perturbations. Mitochondrial ATP loss is validated as an appropriate cardiotoxicity indicator for PFOS and PFOA, thereby advancing the toxicological assessment of emerging pollutants.</p></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic Ag-polyaniline/MXene interfaces for enhanced electrochemical nitrate detection","authors":"Vishwanath Ankalgi,&nbsp;Mohammed Arkham Belgami,&nbsp;Subhashree Das,&nbsp;Chandra Sekhar Rout","doi":"10.1007/s00604-026-08423-7","DOIUrl":"10.1007/s00604-026-08423-7","url":null,"abstract":"<div><p> A V₂CT_x MXene/Ag-doped polyaniline (VCX-PANI-Ag) nanocomposite was successfully synthesized and employed as the sensing interface on a screen-printed carbon electrode (SPCE) for electrochemical nitrate detection. The successful formation of the ternary nanocomposite was confirmed by XRD, FESEM, HRTEM, SAED, UV-Vis, and XPS, demonstrating the uniform integration of crystalline Ag nanoparticles with conductive polyaniline on VCX nanosheets. Owing to the synergistic effects of the conductive MXene framework, electrocatalytically active Ag nanoparticles, and the PANI network, the sensor exhibited two linear detection ranges of 0-2.2 mM and 2.2–12.2 mM, with detection limits of 0.0145 mM and 0.0276 mM, and sensitivities of 0.0871 and 0.0458 mA mM^-1 cm^-2, respectively. The sensor demonstrated excellent selectivity toward nitrate in the presence of Cl^-, NO₂^-, PO₄^3-, HCO₃^-, CH₃COO^-, Ca²⁺, Mg²⁺, K⁺, Na⁺, NH₄⁺, Fe³⁺, Fe²⁺, Mn²⁺, and Ni²⁺ and maintained stable electrochemical performance over 100 cyclic voltammetry cycles. Real-sample testing with tap water, lake water, and soil leachates produced spike-and-recovery values ranged from 50% to 97.5%, with an average RSD of 5.3%, underscoring the sensor’s robustness, precision, and reproducibility under environmentally relevant conditions.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphorylation-asymmetric recombinase polymerase amplification enables target-directed circularization for ultrasensitive multiplex electrochemical detection of respiratory pathogens","authors":"Yemin Han,&nbsp;Haotian Yu,&nbsp;Manman Lv,&nbsp;Mengqing Cheng,&nbsp;Houyang Ge,&nbsp;Song Gao,&nbsp;Lu Yin,&nbsp;Qinyu Ge,&nbsp;Xinping Xu,&nbsp;Yan Huang,&nbsp;Longhua Sun,&nbsp;Zuhong Lu,&nbsp;Quanjun Liu","doi":"10.1007/s00604-026-08419-3","DOIUrl":"10.1007/s00604-026-08419-3","url":null,"abstract":"<div><p>Target-directed circularization has emerged as an efficient strategy for rolling circle amplification (RCA); however, its application to double-stranded DNA (dsDNA) remains challenging because genomic DNA cannot directly generate ligation-ready circular templates. Herein, we report a phosphorylation-asymmetric recombinase polymerase amplification (PA-RPA) strategy that directly converts dsDNA into ligation-ready single-stranded templates, thereby enabling target-directed circularization without requiring predesigned padlock probes. To facilitate multiplex analysis, a universal terminal sequence was incorporated into all PA-RPA products, allowing different targets to share a common bridge oligonucleotide for circular template construction and substantially simplifying assay design. The generated circular templates initiate Phi29 polymerase-mediated RCA, while Cas12a-mediated cleavage releases secondary primers that continuously regenerate amplification intermediates through a positive-feedback circuit. Coupled with a signal-off electrochemical transduction platform based on stem-loop probes immobilized on screen-printed carbon electrodes, the proposed strategy enabled simultaneous detection of <i>Staphylococcus aureus</i> (<i>S. aureus</i>) and <i>Acinetobacter baumannii</i> (<i>A. baumannii</i>). The biosensor achieved detection limits of 0.33 aM and 0.45 aM for <i>S. aureus</i> and <i>A. baumannii</i>, respectively, with a linear range from 1 aM to 10 pM, together with excellent specificity, satisfactory storage stability, and reliable analytical performance in simulated clinical samples. By establishing a general template-conversion strategy for target-directed circularization of dsDNA and a universal circularization design compatible with multiplex analysis, this work expands the applicability of RCA-based molecular diagnostics and provides a versatile analytical framework for CRISPR-powered electrochemical nucleic acid detection.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescence resonance energy transfer-based aptasensor for rapid detection of aflatoxin M1 in milk","authors":"Leiying Wu,&nbsp;Shanxiao Liao,&nbsp;Alideertu Dong,&nbsp;Xue Hu,&nbsp;Zhiyong Lv,&nbsp;Yuchun Guo,&nbsp;Haixia Wu","doi":"10.1007/s00604-026-08437-1","DOIUrl":"10.1007/s00604-026-08437-1","url":null,"abstract":"<div><p>A novel aptasensor for the detection of aflatoxin M1 (AFM1) was developed based on the fluorescence resonance energy transfer (FRET) effect between gold nanoparticles (AuNPs) and FAM-labeled cDNA (FAM-cDNA). Additionally, magnetic nanoparticles (Fe₃O₄ NPs) were attached with AuNPs to construct a Fe₃O₄@AuNPs system that was easy to separate by the external magnetic field. In this aptasensor, FAM-labeled cDNA and Fe₃O₄@AuNPs acted as donor and acceptor, respectively. In the presence of target AFM1, the aptamer of this aptasensor specifically recognized with AFM1, resulting in a turn-on fluorescence signal due to target-induced dissociation of FAM-cDNA from the FRET pair. The morphology, size and functional group of Fe₃O₄@AuNPs were characterized by TEM, XRD and FT-IR. The UV-vis absorption spectroscopy and fluorescence spectroscopy were used to determine the construction of Fe₃O₄@AuNPs@Apt@FAM-cDNA aptasensor. Under optimal conditions, the linear concentration range of 1-100 ng/mL, limit of detection (LOD) of 0.20 ng/mL, and recoveries of 99%~101% of the aptasensor were obtained. Overall, this aptasensor with satisfied sensitivity and selectivity are promising to be applied in the detection of other biochemical analytes in the future.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149102544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNAzyme assisted self-feedback electrochemiluminescence signal amplification for the detection of miRNA-622","authors":"Kaiyue Liu,&nbsp;Shujing Wang,&nbsp;Yajing Zhao,&nbsp;Liyuan Jing,&nbsp;Jingke Zhao,&nbsp;Peixia Li,&nbsp;Li Xie,&nbsp;Shenguang Ge","doi":"10.1007/s00604-026-08417-5","DOIUrl":"10.1007/s00604-026-08417-5","url":null,"abstract":"<div><p>A novel microRNA (miRNA-622) biosensor platform was constructed based on nano-confined effect of vertically-ordered mesoporous silica film (VMSF) and self-feedback cascade amplification strategy of DNAzyme. VMSF was grown in-situ on the ITO electrode surface, which could effectively block the interference of biological matrix and enrich the luminophore by its nano-confinement effect. Meanwhile, DNAzyme walking strands and ferrocene-labeled substrate strands were immobilized on the electrode surface through Au-S bonds, quenched the ECL signal and switched the signal-off. Upon the addition of target miRNA-622, the DNAzyme walker was activated and moved autonomously, completing substrate strand cleavage and signal-on with the assistance of Mg²⁺. The released substrate strands could act as target analogues to reactivate DNAzyme, realizing self-feedback cascade amplification. By organically combining the anti-interference performance of nanomaterials with the signal amplification capacity of DNAzyme, the constructed sensing platform achieved high-sensitivity quantitative detection of miRNA-622 with a linear range spanning from 1 fM to 10 nM and a detection limit as low as 0.65 fM. This study provided a promising approach for the precise determination of low-abundance miRNAs.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149073139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A derivatization-assisted SERS platform using Au TOHs/hydrogel for point-of-use detection of trace ozone in air","authors":"Xinyuan Zhao,&nbsp;Bingxin Xu,&nbsp;Wei Zhang,&nbsp;Haiyan Wei,&nbsp;Yu Zhang,&nbsp;Wenwen Chen,&nbsp;Ming Shang,&nbsp;Cuijuan Wang","doi":"10.1007/s00604-026-08413-9","DOIUrl":"10.1007/s00604-026-08413-9","url":null,"abstract":"<div><p>A portable ozone detection strategy is reported based on derivatization-assisted surface-enhanced Raman spectroscopy (SERS). The strategy integrates active sampling, chemical derivatization, and signal enhancement using high-index gold trisoctahedrons embedded in a porous hydrogel (Au TOHs/hydrogel), assembled into a 48-well microdevice. Ozone is selectively captured by eugenol and converted to formaldehyde, which reacts with 3-methyl-2-benzothiazolinone hydrazone (MBTH) to form a Raman-active azo compound, enabling sensitive detection. The SERS signal at 1270 cm^− 1 exhibited a strong linear correlation with the logarithm of analyte concentration (R² = 0.996). The limit of detection under simulated air conditions is 5.80 × 10^− 6 mg/m³. The SERS sensor demonstrated high stability, spatial uniformity, and reproducibility (RSD &lt; 10%), along with good selectivity against common volatile organic compounds. Validation with air samples and comparison with ultraviolet-visible UV-vis spectrophotometry further supports its applicability. This cost-effective and user-friendly platform shows potential for point-of-use detection of trace ozone.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149068796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZIF-8/β-cyclodextrin composite-based plasmonic sensor for selective detection of copper ions in water","authors":"Hui Zhang,&nbsp;Weisen Peng,&nbsp;Manlin Yu,&nbsp;Can Zou,&nbsp;Kunhua Wen,&nbsp;Miao He","doi":"10.1007/s00604-026-08426-4","DOIUrl":"10.1007/s00604-026-08426-4","url":null,"abstract":"<div><p>The selective detection of different metal-ion pollutants is of great significance for assessing water quality and investigating pollution sources. To address this issue, a composite suspension of zeolitic imidazolate framework-8 and β-cyclodextrin (ZIF-8@β-CD) and functionalized a prism-based surface plasmon resonance (SPR) sensor with the suspension via drop casting has been prepared for the selective detection of Cu²⁺ in water. The interaction between ZIF-8 and Cu²⁺ is mainly related to ion exchange and coordination interaction, while the hydroxyl group on the surface of cavity-structured β-CD confers excellent selective binding ability with Cu²⁺. Based on these combined effects, the maximum resonance wavelength shift of the ZIF-8@β-CD-functionalized SPR sensor was 23.03 nm within a Cu²⁺ concentration range of 0–320 µM, while that was merely 5.12 nm for the sensor modified with ZIF-8 alone. Additionally, the introduction of β-CD produces a synergistic effect with ZIF-8, significantly enhancing the sensor’s selective capture capacity for Cu²⁺ relative to the tested interfering metal ions, including Cd²⁺, Zn²⁺, Cr³⁺, Fe³⁺, Co²⁺, Mn²⁺, Ca²⁺, and Mg²⁺. Consequently, the resonance wavelength shift showed a good linear relationship with Cu²⁺ concentration over the range 0–80 µM, yielding a sensitivity of 0.2 nm/µM and a LOD of 4.35 µM. This study presents a novel, straightforward strategy for functionalizing SPR sensor interfaces to enable high-performance, selective detection of Cu²⁺, with significant value for aquatic ecosystem monitoring and freshwater resource safety.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149035062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A chlorella-derived carbon dots-based integrated sensing-removal platform for silver ion monitoring and removal via dual-mode optical detection and smartphone-assisted deep learning","authors":"Fenglan Li,&nbsp;Xuan Liu,&nbsp;Bifei Huang,&nbsp;Guoxin Zhuang,&nbsp;Jinyuan Chen","doi":"10.1007/s00604-026-08421-9","DOIUrl":"10.1007/s00604-026-08421-9","url":null,"abstract":"<div><p>Chlorella-derived fluorescent carbon dots (CDs) were synthesized via a solvothermal route and a dual-mode colorimetric/fluorescent platform was constructed for Ag⁺ sensing based on coordination-induced visible color change. To enable portable analysis, the CDs were immobilized on filter paper to fabricate portable test strips. Smartphone-based image acquisition, coupled with a convolutional neural network (CNN) model running on a portable laptop, allowed intelligent extraction and quantitative interpretation of color features for Ag⁺ determination. Notably, the coordination/aggregation interaction between CDs and Ag⁺ generated a reddish-brown precipitate, which could be readily removed by centrifugation, achieving an Ag⁺ removal efficiency of over 86.7% and thereby coupling rapid detection with post-detection removal in a two-stage integrated platform. Under optimized conditions, the limits of detection for the fluorescent and colorimetric modes were 0.13 and 0.49 µM, respectively, while the CNN-assisted test-strip assay achieved a limit of detection of 0.88 µM. The recoveries for Ag⁺ in real samples (milk and tap water) ranged from 97.8% to 101.9%, in good agreement with inductively coupled plasma-mass spectrometry (ICP-MS) results. This work presents a functionally integrated, portable, and AI-assisted strategy for rapid Ag⁺ monitoring and removal in water and food-related matrices.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149056803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Portable bioactive capsule-integrated electrochemical-colorimetric dual-mode biosensor based on Mn/Ce MOF nanozyme for rapid detection of malathion in water","authors":"Sirui Li,&nbsp;Shanshan Wang,&nbsp;Guorui Chang,&nbsp;Yaoxing Yang,&nbsp;Hui Feng,&nbsp;Haoqian Xue,&nbsp;Li Zhang,&nbsp;Manlin Luo,&nbsp;Jing Liu,&nbsp;Bowen Li,&nbsp;Shuxia Xu","doi":"10.1007/s00604-026-08398-5","DOIUrl":"10.1007/s00604-026-08398-5","url":null,"abstract":"<div><p>To enable rapid and reliable on-site monitoring of organophosphorus pesticides a novel portable dual-mode biosensor has been developed which integrates electrochemical and colorimetric detection into a single freeze-dried bioactive capsule. This innovative design supports instrument-free operation and provides dual-signal cross-validation. The sensor is based on a newly synthesized Mn/Ce bimetallic metal-organic framework (MOF) nanozyme with high oxidase-like activity, co-immobilized with acid phosphatase (ACP) within a mannitol- and sucrose-stabilized lyophilized capsule to ensure long-term stability and field readiness. In the presence of malathion, the pesticide selectively inhibits ACP, thereby restoring the MOF's catalytic activity and enabling the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). The resulting signal is detected both visually and through chronoamperometry after conversion to a yellow diimine product. This approach achieves a wide linear detection range of 1–2000 nM and an exceptionally low detection limit of 0.06 nM for malathion, along with high accuracy evidenced by recoveries of 93.31%-104.03% in real water samples. This work establishes a field-deployable and user-friendly sensing platform that combines innovative material design with integrated dual-mode detection, offering a robust and practical strategy for on-site environmental monitoring.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149035164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}