Microchimica Acta最新文献

{"title":"Illuminating NF-KB p50 detection: a cascaded electrochemiluminescent biosensor powered by T7 RNA polymerase transcription and DSN-mediated cleavage on an MXene interface","authors":"Zhengfang Liang,&nbsp;Runmin Chen,&nbsp;Haoxu Zhao,&nbsp;Dahong Zhang,&nbsp;Yuanxun Gong,&nbsp;Jiayi Zhang,&nbsp;Qianli Tang,&nbsp;Kai Zhang,&nbsp;Xianjiu Liao","doi":"10.1007/s00604-025-07414-4","DOIUrl":"10.1007/s00604-025-07414-4","url":null,"abstract":"<div><p>Early and sensitive detection of transcription factors such as NF-κB p50 is essential for accurate disease diagnosis and monitoring. We present a highly sensitive electrochemiluminescence (ECL) biosensor for the detection of NF-κB p50, integrating a multifunctional nanocomposite and a three-enzyme signal amplification cascade. The sensing platform is constructed by modifying a glassy carbon electrode with a nanocomposite composed of gold nanoparticles (AuNPs), Ti₃C₂Tₓ MXene, and a Ru(II)-polyethylenimine (PEI) complex, which enables strong and stable ECL emission and efficient probe immobilization. The detection mechanism combines Exonuclease III (Exo III)-mediated target recycling, T7 RNA polymerase-driven transcription, and duplex-specific nuclease (DSN)-facilitated signal amplification. Upon binding of NF-κB p50 to the DNA probe, the T7 promoter is exposed, initiating transcription of abundant RNA strands. These RNAs subsequently act as templates for DSN to cleave Fc-labeled DNA on the electrode surface, releasing quenching tags and restoring the ECL signal. The proposed biosensor exhibits excellent analytical performance, including a wide dynamic range (1 aM–10, pM), a low detection Limit of 0.273 aM, and high specificity against interfering proteins. It also demonstrates strong anti-interference capability and satisfactory recovery in diluted cell lysate samples. This work provides a versatile and scalable biosensing strategy with great potential for clinical diagnostics, real-time disease monitoring, and broader biomarker detection in complex biological matrices.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic cationic covalent organic framework for selective enrichment of phenoxy carboxylic acid herbicides prior to quantification by LC–MS/MS","authors":"Guiju Xu,&nbsp;Xingyun Zhao,&nbsp;Chenghao Hou,&nbsp;Hongwei Zhang,&nbsp;Baoyu Li,&nbsp;Chunlei Yang","doi":"10.1007/s00604-025-07528-9","DOIUrl":"10.1007/s00604-025-07528-9","url":null,"abstract":"<div><p>A magnetic ionic covalent organic framework (iCOF) was prepared with dimidium bromide (DB) as the ionic building blocks and named as Fe₃O₄@DB-iCOF. The Fe₃O₄@DB-iCOF was applied as magnetic solid-phase extraction (MSPE) adsorbent for the enrichment of phenoxy carboxylic acid herbicides (PCAs). Various MSPE parameters including pH, ionic strength, adsorbent dosage, extraction time, eluent type, and eluent volume were optimized. The coexistence interference and recycle experiments confirmed the Fe₃O₄@DB-iCOF possesses the selectivity characteristics of mixed-mode ion-exchange adsorbent and good reusability towards PCAs. The density function theory (DFT) computational adsorption energy verified the adsorption process is exothermic and spontaneous. The molecular dynamics (MD) simulation confirmed the crucial role of electrostatic interaction in driving adsorption process. A novel method for analyzing trace PCAs residues in water samples has been established by combining the Fe₃O₄@DB-iCOF based MSPE with liquid chromatography tandem mass spectrometry (LC–MS/MS). The proposed method exhibits wide linear range (10–2000 ng L⁻¹) and high sensitivity with limits of detection in the range 0.8–1.9 ng L⁻¹. The proposed method was utilized to determine PCAs in river and lake water, with spiked recoveries ranging from 90.8 to 103.1%, confirming its good accuracy.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of malachite green by electrochemical sensor constructed from modified high-affinity aptamer","authors":"Zanlin Chen,&nbsp;Haiming Li,&nbsp;Yian Chen,&nbsp;Shuangyan Han","doi":"10.1007/s00604-025-07506-1","DOIUrl":"10.1007/s00604-025-07506-1","url":null,"abstract":"<div><p>A high-affinity aptamer was developed using a molecular docking approach, leading to the establishment of an electrochemical aptamer biosensor for the detection of malachite green (MG). The aptamer designated as MG-15 was derived from a rational truncation of MG-36, yielding a dissociation constant (K_d) of 45.4 <span>(pm)</span> 17.6 μM. Further enhancements were achieved by completely mutating the binding site of MG-15, resulting in a higher affinity aptamer (MG-15-GGT) with a lower K_d of 2.71 <span>(pm)</span> 1.95 μM. This sensor exhibited a limit of detection (LOD) value of 2.74 pM and demonstrated excellent specificity, with recoveries ranging from 87.1 to 111.5%. Consequently, electrochemical biosensors utilizing modified aptamers hold great promise for applications in food safety.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145100805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A signal-amplifying electrochemical immunosensor based on palladium decorated CoS2 hollow nanoboxes and gold-functionalized Fe3O4 for neuron-specific enolase detection","authors":"Dehao Jia,&nbsp;Yujian Guo,&nbsp;Lei Yang,&nbsp;Xing Gao,&nbsp;Dongquan Leng,&nbsp;Qin Wei","doi":"10.1007/s00604-025-07522-1","DOIUrl":"10.1007/s00604-025-07522-1","url":null,"abstract":"<div><p>A sandwich-type electrochemical (EC) immunosensor for neuron-specific enolase (NSE) detection was constructed utilizing a signal amplification strategy. This strategy was implemented by utilizing palladium decorated CoS₂ hollow nanoboxes (CoS₂-Pd HNBs) as the signal indicator and gold-functionalized Fe₃O₄ (Fe₃O₄-Au) as the EC sensing platform. CoS₂ HNBs with hollow structures were synthesized via template method, which exhibited excellent electrocatalytic performance toward H₂O₂. Moreover, Pd nanoparticles (NPs) were decorated on CoS₂ HNBs by in situ reduction method, which further improved the electrocatalytic performance for H₂O₂ reduction due to the synergetic catalysis. Modifying Au NPs uniformly on Fe₃O₄ surface could not only enhance the electroconductivity of the EC sensing platform but also immobilize the capture antibody (Ab₁) via strong coordination. Fe₃O₄-Au nanocomposites were anchored to the magnetic glass carbon electrode because of good magnetic properties, improving stability of the EC immunosensor. Prominently, the well-established sandwich-type EC immunosensor with NSE as a targeted detection object exhibited a low detection limit of 0.29 pg/mL (S/N = 3) and a linear range from 500 fg/mL to 50 ng/mL. This work may provide a promising detection method for other biomarkers.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smartphone electrochemical sensor based on laser-induced graphene integrated electrode for on-site sulfadimidine detection in beef and milk","authors":"Qian Zeng,&nbsp;Yangping Wen,&nbsp;Weiqiang Li,&nbsp;Hongyu Xiao,&nbsp;Xiaoqin Fu,&nbsp;Zhenna Shi,&nbsp;Anna Li,&nbsp;Jingye Liang,&nbsp;Chaoli Tan,&nbsp;Qihua Xu,&nbsp;Kaijie Tang","doi":"10.1007/s00604-025-07538-7","DOIUrl":"10.1007/s00604-025-07538-7","url":null,"abstract":"<div><p>Sulfadimidine (SM2), a potentially carcinogenic sulfonamides, poses a threat to food safety. In this study, a portable electrochemical sensing platform integrated with a smartphone is developed for on-site sulfadimidine (SM2) detection. The electrode utilizes a flexible three-electrode system based on laser-induced porous graphene (LIPG), fabricated via CO₂ laser etching of polyimide (PI) film. The platform is wirelessly connected to a portable electrochemical workstation and smartphone via Bluetooth. Laser parameters, including power and etching depth, are optimized to improve electrochemical performance. The optimized LIPG electrode exhibits significantly improved sensitivity—2.87 and 10.87-fold higher than screen-printed carbon electrode (SPCE) and glassy carbon electrode (GCE), respectively—along with excellent stability (RSD &lt; 0.46% over 99 cycles) and a rapid 10 s response time. Quantitative detection of SM2 demonstrates good linearity (3 – 110 μM, <i>R</i>² = 0.999), with low limits of detection (LOD) of 0.03 μM and quantification (LOQ) of 0.1 μM. The method is validated in spiked beef (recovery 93.34% – 103.70%) and milk (recovery 98.50% – 100.80%), confirming its practical applicability. This work is novel in systematically linking laser processing parameters to LIPG electrochemical performance and significant in demonstrating a field-deployable, cost-effective, and smartphone-enabled strategy for monitoring SM2 residues.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Versatile functional properties of polyindole/CuO nanocomposites for dielectric and environmental sensing applications","authors":"R. Gopika,&nbsp;M. T. Ramesan","doi":"10.1007/s00604-025-07527-w","DOIUrl":"10.1007/s00604-025-07527-w","url":null,"abstract":"<div><p>Conductive polyindole/copper oxide (PInCuO) nanocomposites (NCs) were synthesized via oxidative in situ polymerization. Fourier-transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) analyses confirmed the successful incorporation of CuO nanoparticles and their crystallinity within the polymer matrix. UV–Vis analysis revealed enhanced optical conductivity, a reduced optical bandgap, and an increased refractive index with CuO addition, showing optimal effects at 7 wt% loading. Field-emission scanning electron microscopy (FE-SEM) images revealed a uniform nanoparticle dispersion at this concentration, whereas higher loadings led to agglomeration. Differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) revealed that the incorporation of CuO significantly improved the phase transition temperature and thermal stability of the NCs. The impact of nanofiller concentration, frequency, and temperature on the electrical, dielectric, and modulus properties was systematically investigated. Dielectric analysis showed a substantial increase in the dielectric constant with increasing filler content, with the NC containing 7 wt% CuO (PInCuO7) exhibiting the highest charge storage capacity. Moreover, PInCuO7 exhibited the highest AC conductivity of 3.5 × 10⁻⁵ S/cm at room temperature and 10⁶ Hz, significantly surpassing that of pristine PIn (2.7 × 10⁻⁶ S/cm). The activation energy from Arrhenius plots was significantly lower for PInCuO7 (0.054 eV) compared to PIn (0.174 eV), indicating enhanced charge carrier mobility due to the effective dispersion of CuO. Additionally, PInCuO NCs exhibited improved ammonia sensing performance over PIn, underscoring the critical role of CuO in enhancing optical, electrical, and sensing properties of the nanocomposites. These findings highlight the potential of PInCuO NCs for advanced applications in optoelectronics, energy storage, and gas sensing.\u0000</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laser-induced WO3-decorated porous graphene for portable and self-powered photoelectrochemical aptasensing","authors":"Lulu Zhang,&nbsp;Lingbo Liu,&nbsp;Jiang Guo,&nbsp;Jingdong Zhang,&nbsp;Kai Yan","doi":"10.1007/s00604-025-07532-z","DOIUrl":"10.1007/s00604-025-07532-z","url":null,"abstract":"<div><p>Photoelectrochemical (PEC) biosensors have attracted significant interest due to their inherent advantages of high sensitivity, excellent biocompatibility, and ease of miniaturization. In this study, efficient WO₃-based photoelectrodes were developed via a novel one-step laser-scribing strategy, enabling the <i>in-situ</i> synthesis of patterned, flexible WO₃-decorated porous graphene electrodes (LIG-WO₃) using H₂WO₄-coated polyimide (PI) film as the precursor. The localized high temperature and reducing atmosphere generated during laser irradiation simultaneously convert the PI into laser-induced graphene and decompose H₂WO₄, yielding photoactive WO₃ and conductive metallic W nanoparticles embedded in the porous graphene matrix. The optimized LIG-WO₃ photoelectrode exhibits a rapid photoelectric response under visible Light illumination at 0 V applied bias, enabling self-powered PEC sensing. A visible light-driven, self-powered PEC aptasensor was fabricated by immobilizing an oxytetracycline (OTC) specific aptamer onto the photoelectrode surface. The Sensor exhibits a Linear response toward OTC in the range 1.0  -1.0 × 10³ nM, with a low detection Limit of 0.68 nM (S/N = 3) as well as high stability, reproducibility and applicability in real sample analysis. This work demonstrates the significant promise of one-step laser-induced WO₃ electrodes for advancing self-powered PEC sensing applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145084772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A ratio-type fluorescence approach based on dual-functional carbon dots with magnetic and luminescent features for IL-8 separation and detection","authors":"Haixia Yang,&nbsp;Yijiong Xue,&nbsp;Tingting Wang,&nbsp;Yan Lu,&nbsp;Minghui Yang,&nbsp;Xiaoqing Li,&nbsp;Ting Li","doi":"10.1007/s00604-025-07542-x","DOIUrl":"10.1007/s00604-025-07542-x","url":null,"abstract":"<div><p>Ratio-based fluorescence assays can reduce environmental interference, allowing for the identification of biomarkers with higher specificity. However, the complicated preparation procedures involved in developing multi-fluorescent emission probes and the challenges associated with isolating and enriching specific biomarkers from complex samples present formidable obstacles. Magnetic nanoparticles enable the quick separation and enrichment of biomarkers, hence, the present study developed a ratio-fluorescence assay for the detection of interleukin-8 (IL-8) using carbon dots (CDs) with both magnetic and fluorescent properties. In this approach, magnetic and fluorescent Fe₃O₄-CDs-1 (FCs) hybrid nanoparticles were initially synthesized, followed by the growth of SiO₂ nanorods on their surface. FCs@SiO₂-Ab₁ was then prepared by immobilizing capture IL-8 antibody (Ab₁) through amino modification onto FCs@SiO₂ to selectively capture and enrich target IL-8 from samples. Subsequently, CDs-2 modified with detection IL-8 antibody (Ab₂) served as IL-8 detection probe, which formed sandwich structure with FCs@SiO₂-Ab₁ in the presence of IL-8. As the concentration of IL-8 increased, the fluorescence intensity of CDs-2 enhanced accordingly while the fluorescence intensity of FCs remained relatively unchanged. This ratio-fluorescence sensing platform exhibits a wide linear range (20-1000 pg·mL⁻¹) and an impressive low detection limit (6.9 pg·mL⁻¹). Furthermore, this method enables the reliable detection of IL-8 in human serum, thereby offering promising prospects for the development of enrichment and detection systems for other biomarkers.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145078873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cd/Zn-regulated POM-based complexes for the electrochemical detection and photocatalytic reduction of Cr(VI)","authors":"Xinyue Zhang,&nbsp;Dongmei Zheng,&nbsp;Yongxia Hou,&nbsp;Aixiang Tian","doi":"10.1007/s00604-025-07504-3","DOIUrl":"10.1007/s00604-025-07504-3","url":null,"abstract":"<div><p>To address the challenge of Cr(VI) pollution in wastewater, based on the unique \"electronic sponge\" properties of polyoxometalates (POMs), we designed and synthesized two redox-active POM-based complexes: [Cd^Ⅱ₂(dm4bt)₄][GeW₁₂O₄₀] (<b>1</b>) and [Zn^Ⅱ₂(dm4bt)₄][GeW₁₂O₄₀] (<b>2</b>) (dm4bt = 2,2’-dimethyl-4,4’-bithiazole). Complex <b>1</b> enables highly sensitive detection of trace Cr(VI) with a detection Limit of 0.21 μM and simultaneously serves as a photocatalyst to reduce Cr(VI) to harmless Cr(III) with 99.85% removal within 15 min. Under the same conditions, complex <b>2</b> exhibits a detection Limit of 0.30 μM and the reduction rate of Cr(VI) decreased to 93.90%. Transition metals modulate material performance by tuning both the crystal structure and band structure of the inorganic–organic hybrid materials. The high efficiency of <b>1</b> originates from the chemical bond network formed between Cd²⁺ and the terminal oxygens of [GeW₁₂O₄₀]⁴⁻, which constructs a rapid electron pathway and optimizes the energy band structure. Complex <b>2</b> exhibits attenuated performance due to restricted electron transport (dominated by hydrogen bond stacking), which is due to the core regulatory effect of the metal coordination modes in governing charge migration. This work overcomes the limitation of electron transport through a structure-performance regulation strategy, providing new ideas for the development of integrated environmental remediation materials.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145078872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellulose nanocrystal/polyacrylic acid nanofiber composite aerogels characterized by 3D porous structure for colorimetric detection of breath acetone","authors":"Xinyang Su,&nbsp;Likun Wang,&nbsp;Yehong Han,&nbsp;Xuelian Xin,&nbsp;Hongyuan Yan,&nbsp;Jiankun Cao","doi":"10.1007/s00604-025-07515-0","DOIUrl":"10.1007/s00604-025-07515-0","url":null,"abstract":"<p>Breath acetone (BrAce) has been validated as a biomarker for diabetes, playing a crucial role in the non-invasive diagnosis of the diabetes. In this study, cellulose nanocrystal/polyacrylic acid nanofiber composite aerogels loaded with thymol blue (CNC/TB@PAA NFAs), featuring a 3D porous structure, were firstly synthesized and employed as a novel gas-sensitive sensor for BrAce detection. The characterization results reveal that cellulose nanocrystals as a reinforcing nanofiller successfully maintain the 3D hierarchical pore structure stability. The more achievable diffusion of target gas through the interconnected pore channels inside the nanofiber aerogels enables rapid contact and interaction between probe molecules immobilized on the interface of nanofiber and target gas. Consequently, this significantly shortens the response time (2-min acetone gas exposure) and enhances sensing sensitivity. The distinctive reaction mechanism between loaded hydroxylamine sulfate and acetone endows CNC/TB@PAA NFAs with heightened selectivity, effectively eliminating interferences of other components in exhaled breath during colorimetric analysis. Additionally, the sensing performance analysis demonstrates a limit of detection and limit of quantification for acetone at 0.0516 ppm and 0.172 ppm, respectively, and a linear range of 0.2–10 ppm with determination coefficient of 0.9946. It is expected that the proposed CNC/TB@PAA NFA-based colorimetric sensor can be applied as a new strategy for daily health management in healthy people as well as a means of ancillary monitoring for patients with diabetes.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145078874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}