Microchimica Acta最新文献

{"title":"Determination of metal oxide and metallic nanoparticles in indoor air samples using mixed cellulose esters filters and spICP-MS: dissolve and shoot","authors":"Carlos Gómez-Pertusa,&nbsp;M. Carmen García-Poyo,&nbsp;Guillermo Grindlay,&nbsp;Ricardo Pedraza,&nbsp;Adela Yañez,&nbsp;Luis Gras","doi":"10.1007/s00604-025-07139-4","DOIUrl":"10.1007/s00604-025-07139-4","url":null,"abstract":"<div><p>In recent years, there has been increasing concern about the adverse health effects of the metallic and metal-containing nanoparticles (NPs) present in indoor environments. Unfortunately, there is no well-stablish method to simultaneously characterize their number and composition. Recently, our research group proposed a strategy for the determination of metallic nanoparticles in air by means spICP-MS based on the aerosol collection on micro-quartz filters and the subsequent extraction using microwave heating in basic media. Although the proposed method allows accurate and precise characterization of NPs, it suffers from practical drawbacks: (i) micro-quartz filter fibers are released into the sample and must be removed prior to analysis to avoid clogging the nebulizer and (ii) the particle distribution detection limits (LOD_size) achieved are not low enough (28 nm). In this work, we evaluate the NPs trapping capabilities and possible fiber release of filters of different nature commonly used for indoor air quality control (polytetrafluoroethylene (PTFE), nylon, polycarbonate, and mixed cellulose ester (MCE) filters) and NPs of different chemical composition (ZrO₂-, TiO₂-, Pt-, AuNPs), size (20–150 nm), and capping agent (citrate, polyethylene glycol, branched polyethyleneimine, and lipoic acid). The results show that MCE is an optimal solution because it is completely dissolved during the microwave heating step and NPs are recovered quantitatively irrespective of their composition and size. The LODs are also improved down to 15 nm and 120 particles per liter of air, low enough to be used for indoor air pollution control. Finally, the proposed method was successfully tested in a simulated (NPs enriched) indoor environment.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07139-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sorbent magnetic levitation in the 3D printed platform","authors":"Olga Mokhodoeva,&nbsp;Valeriia Maksimova,&nbsp;Tatiana Danilova,&nbsp;Denis Trofimov,&nbsp;Artem Krivenko,&nbsp;Valery Shkinev","doi":"10.1007/s00604-025-07116-x","DOIUrl":"10.1007/s00604-025-07116-x","url":null,"abstract":"<div><p>The emerging technologies based on magnetic levitation and 3D printing manufacturing were utilized to create a sorbent retention platform as an alternative to traditional packed columns. The magnetic nanoscale sorbent was suspended in the aqueous solution in a 3D printed sorption cell placed between two permanent magnets. Simulations of hydrodynamic fluid and magnetic field were carried out to optimize the configuration and positioning of the sorption cell. The developed platform ensures uniform distribution of the sorbent in the cell’s volume and retention under dynamic conditions. The efficiency of palladium solid-phase extraction in a flow mode was demonstrated using modified magnetite nanoparticles. A high concentration factor can be achieved using the spiral shape of the cell with 50–100 mg of the suspended sorbent. The results of the spectrometric determination of trace amounts of palladium in natural water are given. An automated system for environmental analysis based on the proposed platform is promising. </p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transfer learning and data augmentation for glucose concentration prediction from colorimetric biosensor images","authors":"Ga-Young Choi,&nbsp;Na-Ri Kim,&nbsp;Da-Young Yu,&nbsp;Taeha Lee,&nbsp;Gyudo Lee,&nbsp;Han-Jeong Hwang","doi":"10.1007/s00604-025-07136-7","DOIUrl":"10.1007/s00604-025-07136-7","url":null,"abstract":"<div><p> A deep learning algorithm is introduced to accurately predict glucose concentrations using colorimetric paper sensor (CPS) images. We used an image dataset from CPS treated with five different glucose concentrations as input for deep learning models. Transfer learning was performed by modifying four established deep learning models—ResNet50, ResNet101, GoogLeNet, and VGG-19—to predict glucose concentrations. Additionally, we attempted to alleviate the challenge of requiring the large amount of training data by applying data augmentation techniques. Prediction performance was evaluated using coefficients of determination (<i>R</i>²), root mean squared error (RMSE), and relative-RMSE (rRMSE). GoogLeNet showed the highest coefficient of determination (<i>R</i>² = 0.994) and significantly lower prediction errors across all concentration levels compared with a traditional machine learning approach (two-sample <i>t</i>-test, <i>p</i> &lt; 0.001). When data augmentation was performed using 20% of the entire training dataset, the mean prediction error was comparable to that of the original entire training dataset. We presented a novel approach for glucose concentration prediction using deep learning techniques based on transfer learning and data augmentation with image data. Our method uses images from CPS as input and eliminates the need for separate feature extraction, simplifying the prediction process.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143801185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porous GNPs assisted LAMP-CRISPR/Cas12a amperometric biosensor as a potential point of care testing system for SARS-CoV-2","authors":"Mohammad Behnam Rad,&nbsp;Seyed Reza Mohebbi,&nbsp;Abbas Yadegar,&nbsp;Hedayatollah Ghourchian","doi":"10.1007/s00604-025-07094-0","DOIUrl":"10.1007/s00604-025-07094-0","url":null,"abstract":"<div><p>A simple and ultrasensitive amperometric biosensor is introduced which has the potential to be applied as a point of care test for SARS-CoV-2 monitoring. It was prepared by integrating the reverse transcription loop-mediated isothermal amplification (RT‑LAMP) and CRISPR/Cas12a nuclease activity on a modified gold screen-printed electrode (GSPE). The GSPE is modified with double-end thiolated oligonucleotide reporters conjugated to porous gold nanoparticles (PGNPs) and inserted into a homemade poly-methyl methacrylate cartridge. This biosensor was integrated with a low-cost electronic kit to make a platform with the potential to be applied as a point-of-care testing system. The PGNPs on the reporters create a dense, negatively charged barrier that repels the redox couple of [Fe(CN)₆]^3−/4− from the GSPE surface. Upon the addition of a real sample, followed by LAMP amplification and Cas12a nuclease activity on disposable GSPE, in the presence of SARS-CoV-2, the single-guide RNA binds to the target sequence and activates Cas12a. The activated Cas12a then cleaves the reporters, releasing the PGNPs. This removal of electrostatic hindrance allows the redox couple of [Fe(CN)₆]^3−/4− to approach the positively charged GSPE, enhancing the amperometric signal. This biosensor offers an outstanding detection limit of 143 zM (~ 86 copies/mL) and a linear response from 4.7 to 7062 aM for SARS-CoV-2 real samples. By using double-end thiolated reporters and porous GNPs, this novel testing system makes it possible to minimize the required sample volume and reagent costs.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical luminescence sensor for the detection of Allure Red: double luminescence cooperative amplification strategy of self-supporting material Zn3Cu2O2 and CdTe@MWNTs","authors":"Ruidan Li,&nbsp;Li Tian,&nbsp;Yujia Song,&nbsp;Yanjia Guo,&nbsp;Guangping Ma,&nbsp;Pengfei Han,&nbsp;Hanyue Jiang,&nbsp;Wenzhuo Wang,&nbsp;Juan Lu","doi":"10.1007/s00604-025-07137-6","DOIUrl":"10.1007/s00604-025-07137-6","url":null,"abstract":"","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143793125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adjustable crystallinity of covalent organic frameworks for electrochemical aptasensing of penicillin","authors":"Haimei Qin,&nbsp;Huaqing Liang,&nbsp;Siyu Liao,&nbsp;Youjiang Tan,&nbsp;Yuehua Huang,&nbsp;Ziao Zong,&nbsp;Rong Wang,&nbsp;Jing Wang","doi":"10.1007/s00604-025-07133-w","DOIUrl":"10.1007/s00604-025-07133-w","url":null,"abstract":"<div><p>Covalent organic frameworks (COFs) show a great potential application in the field of constructing electrochemical aptasensors due to their high specific surface area, abundant pores, and good stability. Crystallinity (i.e., orderliness) is directly related to the structural characteristics of porous frameworks and affects their sensing performance. However, few reports focus on the influence of crystallinity on electrocatalytic aptasensing performance. This work develops copper porphyrin-based COFs with different crystallinity to fabricate electrochemical aptasensors for detecting penicillin. The research results indicate that highly ordered COF has higher specific surface area and ordered pores, which can significantly improve sensing performance.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143786483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Simultaneous enrichment and ultra-high sensitivity detection of multi-structural synthetic cannabinoids in large-volume wastewater","authors":"Rui Cao,&nbsp;Jiawei Chen,&nbsp;Ningjia Pang,&nbsp;Siyu Li,&nbsp;Mengyi Chen,&nbsp;Bin Di,&nbsp;Deli Xiao","doi":"10.1007/s00604-025-07128-7","DOIUrl":"10.1007/s00604-025-07128-7","url":null,"abstract":"<div><p>Nowadays, the abuse of synthetic cannabinoids has aroused the world’s great attention. However, the determination of synthetic cannabinoids in wastewater faces the bottleneck of complex species and very low content. Therefore, this work integrated GO-Fe₃O₄ with an ionic liquid (ILs-GO-Fe₃O₄) to create a mixed hemimicelles magnetic solid-phase extraction (MHMSPE) system for the detection of synthetic cannabinoids (SCs) in wastewater samples using ultrahigh-performance liquid chromatography—tandem mass spectrometry (UHPLC-MS/MS). Because of the multiple interaction sites of mixed hemimicelles’ magnetic solid-phase extraction materials, multi-structural synthetic cannabinoids can be enriched simultaneously. Under the optimized conditions, the limits of quantitation (LOQ) of the method were as low as 10–50 pg/L, lower than other methods. The average recoveries of 18 analytes were in the range 71.23–96.08% with relative standard deviations (RSD) lower than 15% (<i>n</i> = 6). To our knowledge, this is the first development of magnetic solid-phase extraction for large-volume samples pretreatment (1 L) and ultra-high sensitive detection of synthetic cannabinoids (10 pg/L), due to the high enrichment factor (10,000-fold). The extraction process was convenient, accurate, and rapid, and offers significant potential for ultra-high sensitivity detection in large-volume samples, providing a valuable tool for simultaneous broad-spectrum extraction and drug abuse monitoring.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143786430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Light-driven in situ deposited Au nanoparticles on ZnO substrate with ultrasensitive SERS enhancement for molecular detection","authors":"Apichaya Chantaraklud,&nbsp;Chanoknan Rattanabut,&nbsp;Suwussa Bamrungsap,&nbsp;Tanujjal Bora","doi":"10.1007/s00604-025-07118-9","DOIUrl":"10.1007/s00604-025-07118-9","url":null,"abstract":"<div><p>Uniform, reproducible and stable SERS substrates with high detection sensitivity are crucial for their successful commercial applications. Here, we introduce a plasmonic SERS substrate based on gold nanoparticles (AuNPs) fabricated by using a straightforward light-driven in-situ method for highly sensitive molecular detection. A dense array of zinc oxide nanorods (ZnO NRs) was used as a support surface for the in-situ growth of Au nanoparticles (AuNPs). The SERS performance of the fabricated Au-ZnO substrates was evaluated by using rhodamine 6G (R6G) dye as a model Raman probe, where the distribution of the AuNPs on the substrate was found to play an important role defining the SERS activities The Au-ZnO substrates exhibited exceptional homogeneity (RSD = 3.95%), a detection limit (LOD) of 9 × 10^–11 M, and signal enhancement in the order of 10⁶. Additionally, these substrates demonstrated good stability over a period of 4 weeks when stored under standard room conditions, maintaining more than 80% of the initial Raman signal intensity. When tested for antibiotic residue detection in water using amoxicillin as a model antibiotic, the Au-ZnO substrates revealed LOD in the order of 10^–9 M with linear detection over a wide concentration range of amoxicillin in water. The present work offers a straightforward and inexpensive solution-processed fabrication approach for SERS substrates that holds great potential for the development of extremely sensitive and reliable SERS-based detection and sensor systems.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143786473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanomaterials functionalized signal-ON/OFF electrogenerated chemiluminescence biosensor for quantization of trypsin based on target-induced cleavage of peptide","authors":"Shuo Kang,&nbsp;Ronghan Qu,&nbsp;Yuhong Duan,&nbsp;Jiale Yang,&nbsp;Fen Ma","doi":"10.1007/s00604-025-07131-y","DOIUrl":"10.1007/s00604-025-07131-y","url":null,"abstract":"<div><p>Trypsin (TPN) is an important proteolytic enzyme in the digestive system and its abnormal levels are indicative of some pancreatic diseases. As an endopeptidase, TPN can cleave substrate peptide mainly by catalyzing the hydrolysis of the carboxyl side peptide bond of lysine (K) or arginine (R) residues. Based on this hydrolysis cleavage effect, two kinds of nanomaterials functionalized electrogenerated chemiluminescence (ECL) biosensors for the determination of TPN were designed as follows: A signal-ON ECL biosensor was fabricated by attaching substrate peptide (HWR*GWVC, “*” representing the cleavage site, abbreviated as HGC) labeled with ferrocene carboxylic acid (as quencher) onto the surface of NH₂-MIL-53(Al) film which was incorporated with ECL emitting species (bis (2,2′-bipyridine)-4′-methyl-4-carboxybipyridine-ruthenium (N-succinimidylester)-bis (hexafluorophosphate) (Ru complex)). The presence of tryptic cleavage event can eventually lead the Fc complex to leave the electrode and results in the increase of the ECL intensity. HGC labeled with Ru-Ti₃C₂T_<i>x</i>-AuNP complex was used as capture probe and signal probe, which was attached onto NH₂-MIL-53(Al) film modified glassy carbon electrode. A signal-OFF ECL biosensor was built as described above. The presence of TPN lead the Ru-Ti₃C₂T_<i>x</i>-AuNPs to leave the electrode, which resulted in the decreasing ECL intensity. As expected, the fabricated ECL biosensing methods provide excellent sensitivity and selectivity toward the TPN activity. Thus, this strategy shows great potential application in the clinic for diagnosis of TPN-indicating diseases as well as the screening of TPN inhibitor-based anti-cancer drugs.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143769687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A highly selective electrochemical aptasensor for Pb2+ based on molecular imprinting technology and tetrahedral DNA nanostructure","authors":"Jiaqi Han,&nbsp;Nianxin Zhu,&nbsp;Jiahao Sha,&nbsp;Jinyan Cai,&nbsp;Hui Cao,&nbsp;Tai Ye,&nbsp;Liling Hao,&nbsp;Fei Xu","doi":"10.1007/s00604-025-07101-4","DOIUrl":"10.1007/s00604-025-07101-4","url":null,"abstract":"<div><p> A novel electrochemical biosensor for the detection of lead ions (Pb²⁺) with improved specificity and sensitivity was developed. The sensor design incorporated molecular imprinting technology, where chitosan was polymerized on the electrode surface to form a lead-specific cavity structure, thereby enhancing selectivity in complex sample matrices. Meanwhile, the tetrahedral DNA nanostructure was employed as the recognition probe to mitigate the entanglement issues commonly associated with single-stranded DNA, thus improving the sensitivity of the detection. The developed sensor exhibited a linear dynamic range from 0.050 to 2.000 μg/mL, with a limit of detection (LOD) of 0.0034 μg/mL. The aptasensor’s efficacy was verified through the analysis of aquatic samples, demonstrating a high degree of reliability comparable to that of inductively coupled plasma mass spectrometry (ICP-MS).</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}