Microchimica Acta最新文献_第6页

Preparation of ultra-sensitive and selective hydrogen peroxide–based colorimetric sensor using highly exfoliated g-C3N4 nanosheets with peroxidase-like activity 利用具有过氧化物酶样活性的高剥离 g-C3N4 纳米片制备超灵敏和选择性过氧化氢比色传感器。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-29 DOI: 10.1007/s00604-024-06777-4

Mohib Ullah, Roopashree R, Prakash Kanjariya, Mamata Chahar, Jasgurpreet Singh Chohan, Vineet Tirth, Hassan Alqahtani, Ali Algahtani, Tawfiq Al-Mughanam, Li Zhengxin

{"title":"Preparation of ultra-sensitive and selective hydrogen peroxide–based colorimetric sensor using highly exfoliated g-C3N4 nanosheets with peroxidase-like activity","authors":"Mohib Ullah, Roopashree R, Prakash Kanjariya, Mamata Chahar, Jasgurpreet Singh Chohan, Vineet Tirth, Hassan Alqahtani, Ali Algahtani, Tawfiq Al-Mughanam, Li Zhengxin","doi":"10.1007/s00604-024-06777-4","DOIUrl":"10.1007/s00604-024-06777-4","url":null,"abstract":"<div><p> A highly sensitive, portable, rapid, and accurate colorimetric sensing method is presented. It is based upon exfoliated g-C<sub>3</sub>N<sub>4</sub> nanosheets (E–g-C<sub>3</sub>N<sub>4</sub> NSs), having peroxidase nanozyme-like properties. The as-prepared catalyst (E–g-C<sub>3</sub>N<sub>4</sub> NSs) tends to oxidize the colorless tetramethyl-benzidine (TMB) into oxidized-TMB in the presence of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) generating a dark blue color and corresponding ultraviolet visible-spectral changes following a Michaelis–Menten kinetic. The prepared colorimetric sensor exhibited response within the range 0.001–0.450 μM having <i>R</i><sup>2</sup> value of 0.999 and a detective limit (LOD) of 0.15 ± 0.04 nM. Furthermore, the sensor also displayed outstanding selectivity, ample stability (10 weeks), and excellent practicability in real sample applications. All these outstanding properties were highly attributed to the large surface area with exposed actives sites, high surface energy, and large conductive structure of E–g-C<sub>3</sub>N<sub>4</sub> NSs. For comparison of the catalytic study, we have also explored the sensing mechanism of B-g-C<sub>3</sub>N<sub>4</sub>, using the same optimized experimental conditions. Resultantly, we concluded that the proposed sensor (E–g-C<sub>3</sub>N<sub>4</sub> NSs) will gain considerable attention for on-site environmental and health monitoring in future endeavor.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cost-effective chemiresistive biosensor with MWCNT-ZnO nanofibers for early detection of tuberculosis (TB) lipoarabinomannan (LAM) antigen 采用 MWCNT-ZnO 纳米纤维的高性价比化学电阻生物传感器可用于结核病脂联素甘露聚糖 (LAM) 抗原的早期检测

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-29 DOI: 10.1007/s00604-024-06780-9

Dinesh R. Rotake, Jitendra B. Zalke, Harsh V. Gechode, Sandhini M. Peshkar, Shiv Govind Singh

{"title":"Cost-effective chemiresistive biosensor with MWCNT-ZnO nanofibers for early detection of tuberculosis (TB) lipoarabinomannan (LAM) antigen","authors":"Dinesh R. Rotake, Jitendra B. Zalke, Harsh V. Gechode, Sandhini M. Peshkar, Shiv Govind Singh","doi":"10.1007/s00604-024-06780-9","DOIUrl":"10.1007/s00604-024-06780-9","url":null,"abstract":"<div><p>The development of an affordable chemiresistive biosensor enhanced with a multi-walled carbon nanotube–zinc oxide (MWCNT-ZnO) nanofiber composite is presented. The sensor leverages the precise interaction between lipoarabinomannan (LAM) tuberculosis (TB) antigens and antibodies to achieve high sensitivity and specificity. The MWCNT-ZnO nanofibers have a larger surface area and better electrical conductivity, which makes it easier for TB antibodies to stick to them. The binding of LAM TB antigens to the fixed Monoclonal Antibody-MBS320597 induces significant resistance changes in the chemiresistive sensor, enabling accurate TB detection. Performance evaluation reveals a linear detection range from 1.0 to 100.0 pg/mL in the lower concentration range and up to 6.0 ng/mL in the higher concentration range, with a sensitivity of 79.750 mA pg mL<sup>−1</sup> cm<sup>−2</sup> and a lower limit of detection of 40.54 fg/mL. The sensor exhibits a response time of 102 s. Featuring rapid response time and high sensitivity, this biosensor is ideally suited for point-of-care (PoC) applications. The incorporation of MWCNT-ZnO nanofibers shows great potential for enhancing the development of sensitive and cost-effective TB diagnostic tools, which could play a crucial role in advancing global TB control and management efforts.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142540612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecularly imprinted electrochemiluminescence sensor based on luminol functionalized Co-MOF for rifampicin detection 基于发光酚功能化 Co-MOF 的分子印迹电化学发光传感器用于检测利福平

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-29 DOI: 10.1007/s00604-024-06774-7

Shuning Yang, Li Tian, Yongbo Feng, Yujia Song, Ruidan Li, Yanjia Guo, Huiling Li, Chao Li, Juan Lu

{"title":"Molecularly imprinted electrochemiluminescence sensor based on luminol functionalized Co-MOF for rifampicin detection","authors":"Shuning Yang, Li Tian, Yongbo Feng, Yujia Song, Ruidan Li, Yanjia Guo, Huiling Li, Chao Li, Juan Lu","doi":"10.1007/s00604-024-06774-7","DOIUrl":"10.1007/s00604-024-06774-7","url":null,"abstract":"<div><p>A highly sensitive molecularly imprinted electrochemiluminescence (MIECL) sensor was developed for detecting rifampicin (RIF) based on luminol@Co-MOF. Co-MOF had a significant enhancement of ECL signaling in the luminol-O<sub>2</sub> system. Molecular imprinted polymers (MIPs) with the introduction of RIF provide new properties for the specific recognition of RIF. A noteworthy decrease in ECL intensity was observed with higher concentrations of RIF. Consequently, the ECL signal was controlled by RIF elution from and adsorption by the MIP, thus establishing a new method for RIF detection. Under optimal conditions, this sensor exhibited linear detection ranges of RIF between 1.0 × 10<sup>−11</sup> mol L<sup>−1</sup> and 1.0 × 10<sup>−6</sup> mol L<sup>−1</sup>, with a detection limit of 3.3 × 10<sup>−12</sup> mol L<sup>−1</sup> (<i>S</i>/<i>N</i> = 3). The recoveries ranged between 98.1 and 106.0% in fish samples. This method can be used as a sensitive and rapid method to detect RIF in real samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142524345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bifunctional SnO2/NiO/rGO nanocomposite electrode for hydrogen evolution reaction and detection of winter wheat cold-resistant RNA 用于氢进化反应和检测冬小麦抗寒 RNA 的 SnO2/NiO/rGO 双功能纳米复合电极。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-28 DOI: 10.1007/s00604-024-06778-3

Bairui Tao, Jiaxin Guo, Fengjuan Miao

{"title":"Bifunctional SnO2/NiO/rGO nanocomposite electrode for hydrogen evolution reaction and detection of winter wheat cold-resistant RNA","authors":"Bairui Tao, Jiaxin Guo, Fengjuan Miao","doi":"10.1007/s00604-024-06778-3","DOIUrl":"10.1007/s00604-024-06778-3","url":null,"abstract":"<div><p>A convenient, non-toxic, and low-cost tin dioxide (SnO<sub>2</sub>)/nickel oxide (NiO)/reduced graphene oxide (rGO) nanocatalytic electrode was investigated, which can effectively promote the hydrogen evolution reaction and can also be used to construct a sensitive winter wheat cold-tolerant RNA sensor. Due to the good synergy and photocurrent generation between SnO<sub>2</sub> and NiO, which accelerates the electron transfer and catalyzes the hydrolysis reaction, the resultant data for the hydrogen evolution reaction in alkaline environment of this material are very impressive, with cathodic current densities of up to 10 mA cm<sup>−2</sup>. The marvelous heterostructures and photovoltaic properties form a signal amplification system, which enables the nanocomposites to have an excellent linear sensitivity in low-concentration RNA solutions. The single-stranded complementary RNA of the target RNA was immobilized on the surface of the nanocomposites, which enabled the materials to recognize the target RNA under enzyme-free conditions. The test results showed that the modified electrode materials had good single detection performance in a wide linear range from 0.1 nM to 2 mM, with the limit of detection (LOD) of 0.078 nM. The developed nanocomposite electrode has good performance in hydrogen evolution and winter wheat cold-resistant RNA detection, and is a bifunctional device with superior performance.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of D-NCCDs and its application in fluorescent/colorimetric dual-mode discrimination of glutamine enantiomers 制备 D-NCCD 及其在荧光/比色双模式谷氨酰胺对映体鉴别中的应用。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-28 DOI: 10.1007/s00604-024-06788-1

Xuan Liao, Cancan Lu, Yaning Duan, Cuiling Ren, Hongli Chen

{"title":"Preparation of D-NCCDs and its application in fluorescent/colorimetric dual-mode discrimination of glutamine enantiomers","authors":"Xuan Liao, Cancan Lu, Yaning Duan, Cuiling Ren, Hongli Chen","doi":"10.1007/s00604-024-06788-1","DOIUrl":"10.1007/s00604-024-06788-1","url":null,"abstract":"<div><p>A new type of carbon dots (D-NCCDs) was synthesized by 3, 5-diaminobenzoic acid, N,N-dimethyl-o-phenylenediamine, and D-cysteine. The morphology and structure of D-NCCDs were investigated by transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), and FT-IR spectra, and the chirality was characterized by circular dichroism. In the presence of hydrogen peroxide, the fluorescence of D-NCCDs at 487 nm (<i>λ</i><sub>ex</sub> = 410 nm) showed great discrimination ability towards glutamine enantiomers. The ratio of fluorescence intensity (<i>F</i>/<i>F</i><sub>0</sub>) to the concentration of D-Gln showed good linearity in the range 0.5–10 mM, with a detection limits of 0.11 mM. Meanwhile, the color of the solution gradually changed from light yellow to yellow–brown. The UV–Vis absorption ratio (<i>A</i>/<i>A</i><sub>0</sub>) at 410 nm showed good linearity with the concentration of D-Gln in the range 0.5 to 20 mM; the detection limit is 7.7 μM. But the fluorescence and absorbance of D-NCCDs showed no significant change after the addition of L-glutamine. Thus, fluorescence and colorimetry dual-mode discrimination of glutamine enantiomers was achieved. The fluorescence enantioselectivity of Gln (<i>F</i><sub>L-Gln</sub>/<i>F</i><sub>D-Gln</sub>) is 1.62, and the colorimetric enantioselectivity of Gln (<i>A</i><sub>D-Gln</sub>/<i>A</i><sub>L-Gln</sub>) is 2.14. The chiral discrimination mechanism of D-NCCDs to Gln enantiomers was also investigated systematically. This work not only can discriminate glutamine enantiomers with high sensitivity and convenience, but also offers a new strategy for preparing new dual mode chiral nanoprobes.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142520616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel electrochemical sensor based on ꞵ-cyclodextrin/bismuth oxybromide/multi-walled carbon nanotubes modified electrode with in situ addition of tetrabutylammonium bromide for the simultaneous detection and degradation of tebuconazole 基于ꞵ-环糊精/氧溴化铋/多壁碳纳米管修饰电极并原位添加四丁基溴化铵的新型电化学传感器，用于同时检测和降解戊唑醇

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-28 DOI: 10.1007/s00604-024-06797-0

Narumon Wannasri, Pikaned Uppachai, Teeradech Senasu, Suwat Nanan, Praewpan Katrun, Jitlada Vichapong, Nutthaya Butwong, Supalax Srijaranai, Siriboon Mukdasai

{"title":"A novel electrochemical sensor based on ꞵ-cyclodextrin/bismuth oxybromide/multi-walled carbon nanotubes modified electrode with in situ addition of tetrabutylammonium bromide for the simultaneous detection and degradation of tebuconazole","authors":"Narumon Wannasri, Pikaned Uppachai, Teeradech Senasu, Suwat Nanan, Praewpan Katrun, Jitlada Vichapong, Nutthaya Butwong, Supalax Srijaranai, Siriboon Mukdasai","doi":"10.1007/s00604-024-06797-0","DOIUrl":"10.1007/s00604-024-06797-0","url":null,"abstract":"<div><p>A novel electrochemical sensor–based glassy carbon electrode (GCE) was fabricated and applied to simultaneous detection and degradation of tebuconazole (TBZ) for the first time. The GCE was consecutively modified by multi-walled carbon nanotubes (MWCNTs), bismuth oxybromide (BiOBr), ꞵ-cyclodextrin (ꞵ-CD), and in situ addition of tetrabutylammonium bromide (TBABr). The detection was based on the decreasing of Bi signal at its anodic potential (<i>E</i><sub>pa</sub>) of 0.05 V. Under the optimum conditions, the modified electrode exhibited a linear response to TBZ in the concentration range 1–100 μg L<sup>−1</sup> with a detection limit of 0.9 μg L<sup>−1</sup>. TBZ was firstly adsorbed on the surface of the modified electrode through host–guest molecule interactions of the ꞵ-CD. The adsorption was further enhanced by the large surface area of BiOBr and MWCNTs. The adsorbed TBZ on the electrode surface hindered the electron transfer of Bi, thus decreasing the oxidation of Bi. In addition, the in situ addition of tetrabutylammonium bromide (TBABr) enriched TBZ via electrostatic interactions, increasing its detection sensitivity. The fabricated electrochemical sensor was applied to determine TBZ in water and soil samples from rice fields with recoveries of 80.5–100.5% and 87.6–112%, respectively. Furthermore, the degradation of TBZ on the modified electrode was studied under a solar light simulator. The degradation percentage (100%) of TBZ (50 µg L<sup>−1</sup>) was achieved in 5 min owing to the excellent photocatalytic properties of BiOBr.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142518846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Persistent chemiluminescence-based nanosensor for portable point-of-care testing of H2O2 用于便携式 H2O2 床旁检测的基于持久化学发光的纳米传感器

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-28 DOI: 10.1007/s00604-024-06794-3

Yueliang Wang, Xiying Chen, Zhanhui Tao, Jingya Zhou, Hanyue Sun, Xian Li, Liyu Shi, Yaqing Liu

{"title":"Persistent chemiluminescence-based nanosensor for portable point-of-care testing of H2O2","authors":"Yueliang Wang, Xiying Chen, Zhanhui Tao, Jingya Zhou, Hanyue Sun, Xian Li, Liyu Shi, Yaqing Liu","doi":"10.1007/s00604-024-06794-3","DOIUrl":"10.1007/s00604-024-06794-3","url":null,"abstract":"<div><p> A novel glow-type chemiluminescence (CL)-based nanosensing system was developed for sensitive and rapid point-of-care testing (POCT) of H<sub>2</sub>O<sub>2</sub> in food. CuSe nanoparticles (CuSe<sub>NPs</sub>) have excellent peroxidase-like activity. After being modified with thiols of 4-mercaptophenylboronic acid (MBA) (CuSe<sub>NPs</sub>@MBA), luminol can be catalyzed to produce long-lasting CL in the presence of H<sub>2</sub>O<sub>2</sub>. The possible reason for the long-lasting glow-type CL behavior was explored. Under the optimized condition, H<sub>2</sub>O<sub>2</sub> can be sensitively detected with improved repeatability. The limit of detection is as low as 0.30 μM. To meet the requirement of in situ and outside of laboratory detection, a 3D-printed portable device was designed which can eliminate the environmental interference to improve detection accuracy. The developed multifunctional platform also has the advantages of simple operation and low cost, suggesting its great potential for applications in food and agricultural fields.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142519150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple strategy for dual-responsive ratio electrochemical-colorimetric detection of nitrite in food and environment 食品和环境中亚硝酸盐双响应比电化学比色法检测的简单策略

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-27 DOI: 10.1007/s00604-024-06793-4

Peng Lei, Ni Wu, Ying Zhou, Chuan Dong, Minglu Li, Shaomin Shuang

{"title":"Simple strategy for dual-responsive ratio electrochemical-colorimetric detection of nitrite in food and environment","authors":"Peng Lei, Ni Wu, Ying Zhou, Chuan Dong, Minglu Li, Shaomin Shuang","doi":"10.1007/s00604-024-06793-4","DOIUrl":"10.1007/s00604-024-06793-4","url":null,"abstract":"<div><p>A dual-responsive ratio electrochemical-colorimetric method for nitrite (NO<sub>2</sub><sup>−</sup>) is established based on the combination of nanoenzyme (Mn<sub>3</sub>O<sub>4</sub>) catalysis with diazotization reactions. The Mn<sub>3</sub>O<sub>4</sub> can oxidize colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue TMBox. The NO<sub>2</sub><sup>−</sup> induces the diazotization reaction of TMBox, leading to a decrease of the signal at 652 nm and the generation of a new signal from diazotized TMBox at 445 nm. Furthermore, the presence of NO<sub>2</sub><sup>−</sup> reduces the electrochemical oxidation signal of TMB and simultaneously provides its electrochemical signal. Compared with traditional single-mode detection, dual-mode detection offers higher sensitivity, lower detection limits, and better interference resistance. The inherent advantages of this method make it feasible to detect NO<sub>2</sub><sup>−</sup> in real samples, offering broad prospects for applications in food safety and environmental monitoring.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuning multispectral fluorescence quantum dot–based identification of short-length amyloid β peptides by applying Cu(II) ions 利用 Cu(II) 离子调谐基于多光谱荧光量子点的短长淀粉样β肽鉴定。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-26 DOI: 10.1007/s00604-024-06764-9

Klaudia Głowacz, Weronika Tokarska, Anita Olechowska, Nina E. Wezynfeld, Patrycja Ciosek-Skibińska

{"title":"Tuning multispectral fluorescence quantum dot–based identification of short-length amyloid β peptides by applying Cu(II) ions","authors":"Klaudia Głowacz, Weronika Tokarska, Anita Olechowska, Nina E. Wezynfeld, Patrycja Ciosek-Skibińska","doi":"10.1007/s00604-024-06764-9","DOIUrl":"10.1007/s00604-024-06764-9","url":null,"abstract":"<div><p>Currently available methods for detecting amyloid β (Aβ) derivatives are mainly dedicated to determining the long forms Aβ<sub>1-42</sub> and Aβ<sub>1-40</sub>. At the same time, the number of physiologically occurring Aβ analogs is much higher, including those truncated at the N- and C-termini. Their identification using standard methods is challenging due to the structural similarity of various Aβ analogs, but could highly benefit from both biomarkers discovery and pathophysiological studies of Alzheimer’s disease. Therefore a “chemical tongue” sensing strategy was employed for the detection of seven Aβ peptide derivatives: Aβ<sub>1-16</sub>, Aβ<sub>4-16</sub>, Aβ<sub>4-9</sub>, Aβ<sub>5-16</sub>, Aβ<sub>5-12</sub>, Aβ<sub>5-9</sub>, Aβ<sub>12-16</sub>. The proposed sensing system is based on competitive interactions between quantum dots, Cu(II) ions, and Aβ peptides, providing unique fluorescence fingerprints useful for the identification of analytes. After carefully evaluating the Aβ sample preparation protocol, perfect determination of all studied Aβ peptides was achieved using partial least square–discriminant analysis (PLS-DA). The developed PLS-DA models are characterized by excellent accuracy, sensitivity, precision, and specificity of analyte determination, emphasizing the potential of the proposed sensing strategy.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06764-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fluorescent sensor for rapid and quantitative aquatic bacteria detection based on bacterial reactive oxygen species using Ag@carbon dots composites 基于细菌活性氧的 Ag@carbon dots 复合材料快速定量检测水生细菌的荧光传感器。

IF 5.3 2区化学

Microchimica Acta Pub Date : 2024-10-25 DOI: 10.1007/s00604-024-06783-6

Ruilin Xiao, Haixia Liang, Baohua Tian, Xinxin Li, Tingshan Song

{"title":"A fluorescent sensor for rapid and quantitative aquatic bacteria detection based on bacterial reactive oxygen species using Ag@carbon dots composites","authors":"Ruilin Xiao, Haixia Liang, Baohua Tian, Xinxin Li, Tingshan Song","doi":"10.1007/s00604-024-06783-6","DOIUrl":"10.1007/s00604-024-06783-6","url":null,"abstract":"<div><p>A novel fluorescent sensor based on silver nanoparticle-carbon dot composites (Ag@CDs) has been developed for the rapid and quantitative detection of aquatic bacteria. The sensor operates on the principle of plasmon-enhanced resonance energy transfer, where the fluorescence of CDs is quenched by Ag nanoparticles and restored upon bacterial interaction due to the generation of reactive oxygen species. The Ag@CDs exhibit a linear response to bacterial concentration over the range 7 × 10<sup>4</sup> ~ 4 × 10<sup>7</sup> CFU·mL<sup>−1</sup>, with a low detection limit of 4 × 10<sup>4</sup> CFU·mL<sup>−1</sup>. The fluorescence recovery is rapid, reaching maximum intensity within 5 min. The method demonstrates high selectivity, with minimal interference from common ions and compounds found in municipal and industrial wastewater. The Ag@CDs-based 96-well plate assay for quantitative measurement of bacteria was developed. The assay’s performance was further validated through the analysis of real water samples, showing a recovery of 94.0 ~ 102% for domestic wastewater and 97.6 ~ 106% for industrial wastewater. Also, Ag@CDs-based test strips assay for semi-quantitation were developed for rapid in-field aquatic bacteria detection. Ag@CDs can be conveniently integrated into 96-well plates and test strips, providing rapid on-site aquatic bacteria testing.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 11","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06783-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142492615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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