Microchimica Acta最新文献

{"title":"Silica-based optical chemosensors for rapid and reliable on-site detection of gamma-hydroxybutyrate in beverages and oral fluids","authors":"Jordi Hernández-Contreras,&nbsp;Jordi Roig-Rubio,&nbsp;Margarita Parra,&nbsp;Salvador Gil,&nbsp;Pau Arroyo,&nbsp;José A. Sáez,&nbsp;Carlos Lodeiro,&nbsp;Pablo Gaviña","doi":"10.1007/s00604-025-07134-9","DOIUrl":"10.1007/s00604-025-07134-9","url":null,"abstract":"<div><p>The illicit use of gamma-hydroxybutyrate (GHB) in drug-facilitated crimes underscores the urgent need for rapid and reliable detection technologies. This study introduces two innovative silica-based nanosensors that offer real-time, on-site detection of GHB in spiked beverages at concentrations typical of chemical submission cases. Both chemosensors are based on silica nanoparticles functionalized with 2-aminonaphtoxazole derivatives. The first nanosensor, modified with a <i>p</i>-nitroaniline chromogenic unit, enables a visible color change for immediate visual identification, while the second incorporates a fluorescein derivative for high-sensitivity detection via fluorescence. Both nanosystems demonstrate exceptional specificity to GHB, with minimal interference from other substances. Moreover, the solid-phase integration of these nanosensors onto hydrophobic cellulose-based substrates enhances their portability and user-friendliness, making them suitable for nightlife venues and forensic applications. Finally, chromogenic precursor <b>1</b> has proven to be an excellent chemosensor for the visual detection of GHB in oral fluid, with a calculated LOD of 19.2 μM, and a linear response in the 32–132 μM range.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 5","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07134-9.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143761812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cauliflower-like CuO-ZnO modified glassy carbon electrode as an electrochemical platform for the detection of hydrogen peroxide in adulterated milk","authors":"Haiyan Song,&nbsp;Haobin Hu,&nbsp;Zhijun Li,&nbsp;Yun Wu,&nbsp;Chunxiao He,&nbsp;Yuanyuan Gao,&nbsp;Nana Hou,&nbsp;Qiqi He,&nbsp;Zhenyu Cheng","doi":"10.1007/s00604-025-07130-z","DOIUrl":"10.1007/s00604-025-07130-z","url":null,"abstract":"<div><p>The detection of hydrogen peroxide (H₂O₂) is a crucial process in various industries, including food safety. In this study, a cauliflower-like CuO-ZnO composite with p–n junction was successfully synthesized using a straightforward one-pot hydrothermal method. The resulting material demonstrates outstanding electrocatalytic performance for H₂O₂ detection, outperforming the individual components. The electrochemical sensor constructed from the cauliflower-like CuO-ZnO composite exhibits a well-defined linear response in the concentration range 0.0005 to 0.105 mmol·L⁻¹ for H₂O₂. The sensor’s limit of detection (LOD) is 0.21 µmol·L⁻¹ at a signal-to-noise ratio (S/N) of 3, with a sensitivity of 1582.7 µA·(mmol·L⁻¹)⁻¹·cm⁻². Furthermore, the sensor has been successfully applied to detect trace amounts of H₂O₂ in milk samples, showcasing its potential for sensitive electrochemical sensing applications.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assembly of adaptive engineering aptamers for Escherichia coli and their application in all-in-one rapid detection","authors":"Mengyue Liu,&nbsp;Shicai Xu,&nbsp;Giovanna Marrazza","doi":"10.1007/s00604-025-07138-5","DOIUrl":"10.1007/s00604-025-07138-5","url":null,"abstract":"<div><p>Multivalent engineering aptamers (multi-Apts) adaptive and specific to whole <i>Escherichia coli</i> (<i>E. coli</i>: CMCC 44102) cells based on hybridization chain reaction (HCR) were constructed for the first time. The dissociation constant (<i>K</i>_d) value for these multi-Apts was 48 nM, demonstrating a higher affinity than that of monovalent aptamers (mono-Apts) (<i>K</i>_d = 102 nM). Furthermore, the reaction equilibrium of multi-Apts was achieved within 20 min, with a reaction rate twice that of mono-Apts. To validate the exceptional performance of these multi-Apts, they were employed as recognition elements in conjunction with gold nanoparticles (AuNPs) colorimetric assays for the all-in-one rapid detection of <i>E. coli</i>. This method exhibited a linear detection range from 1 × 10² to 1 × 10⁷ CFU mL⁻¹, achieving a limit of detection (LOD) as low as 19 CFU mL⁻¹. The recovery of this method in tap water and milk were 85.7% to 101% and 81.8% to 98.2%, respectively. The results indicated that this method not only provided a wide detection range but also exhibited high sensitivity and accuracy. Additionally, this study demonstrated that multi-Apts possessed greater application potential in the detection of macromolecular substances such as bacteria. In short, this work provided a novel approach for rapid and all-in-one detection of <i>E. coli</i> in food.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Design and application of graphene oxide-based molecularly imprinted covalent organic framework for simultaneous and precise recognition of bile acid metabolites","authors":"Yue Yuan,&nbsp;Tianyi Xue,&nbsp;Mengxin Ren,&nbsp;Yanzhu Liu,&nbsp;Zhexue Song,&nbsp;Zhili Xiong,&nbsp;Feng Qin","doi":"10.1007/s00604-025-07120-1","DOIUrl":"10.1007/s00604-025-07120-1","url":null,"abstract":"<div><p> In this study a bile acid (BA)-imprinted covalent organic framework (COF) was constructed via Schiff base reactions, which integrated the advantages of both inherent structural stability of COF and exceptional selectivity of molecular imprinting technology. Besides, it was found that the addition of graphene oxide (GO) effectively increased the dispersibility of nanoparticles, ultimately resulting in a GO-based molecularly imprinted COF (GO@MICOF). The GO@MICOF was identified with the characteristics of excellent mass transfer performance (20.09 mg g^–1), specific surface area (152.35 m² g^–1), selectivity (IFs = 2.4), and regeneration ability (<i>n</i> ≥ 10). By coupling the GO@MICOF-based pretreatment method with ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) analysis, sensitive and accurate validation results (LOQs, 0.01–2.5 µmol L^–1; extraction efficiency, 81.1–118.9%) were obtained. Notably, the application of the proposed pretreatment techniques to metabolomics analysis holds great significance for the precision of metabolomics results. Sixteen BA metabolites were successfully quantified in rat liver and fecal samples, and some potential biomarkers and related metabolic pathways associated with postmenopausal osteoporosis had been identified. Therefore, the integrated analysis strategy has significant advantages in purifying complex biological samples and has great application prospects in targeted metabolomics research.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Handling electric connections in 3D-printed electrodes and sensors","authors":"Ivan Verlangieri,&nbsp;Thawan Gomes de Oliveira,&nbsp;Fernando Silva Lopes,&nbsp;Ivano Gebhardt Rolf Gutz,&nbsp;Lúcio Angnes,&nbsp;Claudimir Lucio do Lago","doi":"10.1007/s00604-025-07122-z","DOIUrl":"10.1007/s00604-025-07122-z","url":null,"abstract":"<div><p>Voltammetric and amperometric sensors typically consist of an electroactive surface, an electrode substrate, and connection tracks. While metal connectors exhibit negligible resistance, semiconductor materials, conductive polymers, or composites can introduce significant electrical resistance. This study investigates the electrical behavior of 3D-printed conductive polymer tracks and metal connections, focusing on limitations and improvements. Carbon black PLA (CB-PLA) was chosen for its favorable electrical properties. Printed tracks showed higher resistivity (17 Ω·cm) than the raw filament (6 Ω·cm). The electrical contact resistance (ECR) between nickel-plated metals and CB-PLA ranged from 10² to 10³ Ω. Pressed contacts (e.g., alligator clips) were unstable and introduced noise, while a welded metal-polymer contact (WMPC), achieved via induction heating, improved stability. Despite high resistivity, the electrochemical behavior remained unaffected, apart from an Ohmic drop requiring compensation for accurate sensor performance. To address this, a four-electrode potentiostat was proposed for dynamic Ohmic drop compensation. Cyclic voltammetry experiments were performed using a custom 3D-printed electrode with dual conducting tracks to independently monitor potential and current. Results from a commercial four-electrode potentiostat were compared with those from a conventional three-electrode system. A four-electrode potentiostatic module (FEPM) was developed for compatibility with standard three-electrode instruments, yielding comparable results. Peak current varied linearly with hexaammineruthenium(III) concentration (R² = 0.992) and with the square root of the scan rate (R² ≥ 0.993). Differential pulse voltammetry confirmed enhanced performance with the four-electrode setup. These findings highlight key considerations for integrating 3D-printed components into electrochemical systems and mitigating ECR and Ohmic drop.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laccase-mimicking enzyme with fluorescent properties for dual-mode detection of epinephrine and degradation of phenolic pollutants","authors":"Huizhu Fan,&nbsp;Luxi Huang,&nbsp;Yin Dai,&nbsp;Hongsong Zhang,&nbsp;Wanying Zhu,&nbsp;Jun Zhang,&nbsp;Junli Hong","doi":"10.1007/s00604-025-07069-1","DOIUrl":"10.1007/s00604-025-07069-1","url":null,"abstract":"<div><p>Despite considerable interest has been shown in research on nanozymes in recent times, the creation of highly active bifunctional nanozymes remains a significant challenge. Inspired by the structural features of the active center of natural laccase, bifunctional copper-based nanozymes (Cu-MB) possessing both laccase-like activity and fluorescence properties using 2-methylimidazole (2-MI) and 2-aminoterephthalic acid (BDC-NH₂) as ligands have been prepared. The <i>K</i>_m of Cu-MB was 0.0898 mM, which was markedly lower than that of previously reported nanozymes and natural laccase. A dual-mode sensor for the detection of epinephrine (EP) was designed based on the high catalytic activity, excellent fluorescence properties, and stability of Cu-MB nanozymes. The limits of detection (LODs) of the colorimetric and fluorescence sensors for EP were 0.22 µg/mL and 0.11 µg/mL, respectively. The sensor system was verified to have good resistance to interferences and reliability by analyzing actual samples. Furthermore, the integration of a dual-reading hydrogel with a smartphone facilitates the portable, real-time, and rapid assessment of EP. More encouragingly, Cu-MB has excellent substrate versatility and can effectively degrade a diverse array of phenolic pollutants. The developed Cu-MB nanozymes provide a promising approach for the degradation and determination of phenolic compounds. Clinical trial number: not applicable.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel strategy for rapid, portable, and sensitive detection of Clostridium perfringens cpb2 gene using smartphone-based electrochemical DNA biosensor based on screen-printed electrodes modified with nanocomposite","authors":"Qianyue Wu,&nbsp;Beiying Yang,&nbsp;Yutang Wang,&nbsp;Yanyan Xiong,&nbsp;Jinhua Zhang,&nbsp;Yangping Wen,&nbsp;Andreja Rajkovic","doi":"10.1007/s00604-025-07126-9","DOIUrl":"10.1007/s00604-025-07126-9","url":null,"abstract":"<div><p>An accurate, sensitive, low-cost, portable, and easy-to-use method for the quantitative detection of <i>Clostridium perfringens</i> (Cp), a zoonotic pathogen widely found in nature and capable of spreading through contaminated food or environments, is essential for epidemiology, prevention, and diagnostics. Here, we have designed a smartphone-based electrochemical DNA biosensor, which utilizes carboxyl-functionalized multi-walled carbon nanotubes (COOH-MWCNTs) as the substrate, L-cysteine (L-Cys) as the linker, and a synergistic modification of the bioelectrode with gold nanoparticles (AuNPs) and polyamidoamine dendrimers (PAMAM). This design enables highly sensitive, low-cost, label-free, and portable detection of the cpb2 gene in Cp. The sensor not only enhances detection performance but also improves convenience and practicality. Differential pulse voltammetry (DPV) was used to monitor the electrochemical signal response to changes in target DNA concentration, enabling the tracking of the DNA hybridization process. Under optimized conditions, the biosensor exhibited a linear detection range from 10⁻¹⁴ to 10⁻⁸ M, with a detection limit of 1.5 fM. It demonstrated excellent selectivity for the cpb2 gene and was successfully applied to detect variations in cpb2 gene content in wastewater and fecal DNA samples. Compared with the traditional method, the detection time of this method is short, the operation of professional and technical personnel is not required, the instrument is small and portable, and the single detection cost is significantly reduced. This study provides a new strategy for the rapid, portable, and highly sensitive detection of bacterial toxin genes in livestock and aquaculture.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143717023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel ZIF-67-derived Co3O4 hollow nanocages as efficient nanozymes with intrinsic dual enzyme-mimicking activities for colorimetric sensing","authors":"Jingtian Chi,&nbsp;Jingfei Hu,&nbsp;Rongrong Jin,&nbsp;Shanding Zhou,&nbsp;Siyu Wen,&nbsp;Quantong Jiang,&nbsp;Peng Ju,&nbsp;Xiaofan Zhai","doi":"10.1007/s00604-025-07112-1","DOIUrl":"10.1007/s00604-025-07112-1","url":null,"abstract":"<div><p>Nanozymes with multifaceted functionalities have accrued substantial interest as they provide an expanded spectrum of applications in comparison to their single-active nanozymes. In this endeavor, novel Co₃O₄ hollow nanocages (COHNs) derived from ZIF-67 were crafted adorned with the exceptional quality of dual-enzymatic prowess by employing a simple co-precipitation and pyrolysis technique, all the while meticulously exploring the intricacies of the catalyst mechanism. Kinetic analyses ascertained that the catalytic behavior of COHNs adhered to the archetypal dynamics of Michaelis–Menten, displaying a higher affinity for 3,3′,5,5′-tetramethylbenzidine (TMB) compared to natural enzymes. Leveraging the exceptional peroxidase- and oxidase-mimicking activity of the COHNs, a visual colorimetric assay platform was established for the detection of H₂O₂, ascorbic acid (AA), and acid phosphatase (ACP), all of which showed high selectivity and good sensitivity. Significantly, by harnessing the enzyme mimic property of COHNs, quantitative detection of H₂O₂, AA, and ACP unveiled astoundingly low detection limits of 0.0046 µM, 0.15 µM, and 0.0068 mU∙mL⁻¹, respectively. Moreover, the successful detection application in real samples attested to the superior stability and anti-interference ability of the colorimetric sensing system. This study not only provides a novel nanozyme boasting remarkably dual-enzymatic prowess, but also pioneers a rapid and sensitive method for environmental analysis and clinical diagnosis.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143716811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MIL-125@ZIF-8 based solid phase microextraction coating for sensitive determination of organophosphate esters in aqueous environments","authors":"Xingru Hu,&nbsp;Jiahui Hou,&nbsp;Long Pang,&nbsp;Zhigao Feng,&nbsp;Junhao Liu,&nbsp;Haiyang Feng,&nbsp;Ying Kong,&nbsp;Chao Liu","doi":"10.1007/s00604-025-07127-8","DOIUrl":"10.1007/s00604-025-07127-8","url":null,"abstract":"<div><p>A novel MOF-on-MOF heterostructure (MIL-125@ZIF-8) was synthesized and utilized as a coating material for solid-phase microextraction (SPME), which was subsequently coupled with gas chromatography (GC) for the sensitive determination of organophosphate esters (OPEs) in water samples. Compared to commercial fiber, the MIL-125@ZIF-8 coated fiber (MIL-125@ZIF-8-F) demonstrated superior extraction performance for medium-polar or non-polar compounds due to multiple interactions such as hydrophobic interaction, π-π stacking, and hydrogen bonding. The synergistic effect of MIL-125 and ZIF-8 endowed the MIL-125@ZIF-8-F with high efficiency and excellent stability under extreme conditions. Specifically, the developed MIL-125@ZIF-8-F could withstand 80 conditioning cycles without significant loss of extraction capacity. Moreover, the established method exhibited a wide linear range (10–1000 μg L⁻¹), low detection limits (0.03–0.20 μg L⁻¹), and excellent reproducibility under optimized condition. Finally, this proposed method provided a promising approach for the efficient enrichment of trace OPEs in actual environmental water samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A flexible magnetic DNA biosensor composed of AgNWs/hydrogel/PS/Fe3O4 for the detection of ASFV P72 protein gene fragment","authors":"Mengjie Xue,&nbsp;Shengbo Sang,&nbsp;Dong Zhao,&nbsp;Qianqian Duan,&nbsp;Xing Guo","doi":"10.1007/s00604-025-07114-z","DOIUrl":"10.1007/s00604-025-07114-z","url":null,"abstract":"<p>African swine fever virus (ASFV) is a highly infectious and pathogenic DNA virus with a mortality rate of nearly 100%. Here, we developed a flexible biosensor based on a AgNWs/hydrogel/PS(polystyrene)/Fe₃O₄ composite film for the detection of ASFV P72 protein gene fragments. A large number of carboxyl groups in the hydrogel provide modification sites for capture probes (cp) simplifying the preparation process of the biosensor. Combined with the excellent magnetic properties of Fe₃O₄, the excellent electrical conductivity of silver nanowires (AgNWs) and the unique optical properties of two-dimensional photonic crystals (2DPC), the flexible biosensor can convert biological signals into optical signals and electrical signals, and the micro-deformation of the film can be verified by simple optical methods. In order to improve the sensitivity, we introduce Fe₃O₄ and apply an external magnetic field to amplify the thin film micro-deformation. The concentration of P72 protein gene fragment and the relative change rate of resistance showed a good linear relationship, and the linear equation is <i>y</i> = − 0.00126x − 0.31729; the detection limit (LOD) is as low as 0.208 μM. The composite film was used to detect real serum samples, and the recovery of the composite film fluctuated in the range 91.89 to 103.19%, indicating that the composite film has practical application potential in clinical detection of ASFV. In addition, the biosensor also shows good biocompatibility, stability, and specificity.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143726647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}