Microchimica Acta最新文献_第6页

Ni-C@MOF-Apt microspheres with enzyme activity and photodynamic properties for capture and clearance of MCF-7 cells Ni-C@MOF-Apt具有酶活性和光动力学特性的微球，用于捕获和清除MCF-7细胞

IF 5.4 2区化学

Microchimica Acta Pub Date : 2026-09-25 DOI: 10.1007/s00604-026-08414-8

Qilong Hao, Yi Yang, Shuai Mu, Xiaoyan Liu, Yanping Shi, Haixia Zhang

{"title":"Ni-C@MOF-Apt microspheres with enzyme activity and photodynamic properties for capture and clearance of MCF-7 cells","authors":"Qilong Hao, Yi Yang, Shuai Mu, Xiaoyan Liu, Yanping Shi, Haixia Zhang","doi":"10.1007/s00604-026-08414-8","DOIUrl":"10.1007/s00604-026-08414-8","url":null,"abstract":"<div><p>Circulating tumor cells (CTCs) carry a migration risk, prone to forming metastatic foci and exacerbating the disease. Therefore, it is of great importance to improve the capture efficiency and killing effect of CTCs. First, Ni-doped covalent organic framework (Ni-COF) is used as a precursor for calcination at 900 °C, which reduces Ni<sup>2+</sup> to magnetic Ni nanoparticles, and COF evolve into carbon nanospheres exhibiting enzyme activity (Ni-C-900). Then, with the photosensitizer meso-Tetrakis (4-carboxyphenyl) porphyrin (TCPP) as the first ligand and (2,5-dicarboxyphenyl) boronic acid (DCBA) as the second ligand, a metal-organic framework shell is in-situ grown on the surface of Ni-C-900 to obtain Ni-C@MOF. Finally, the aptamers are orderly linked via DCBA ligands to obtain Ni-C@MOF-Apt microspheres. Ni-C@MOF-Apt exhibits excellent targeting towards MCF-7 cancer cells, with a capture efficiency of 82.6% in whole-blood samples (1000 cells per milliliter), and the capture efficiency can still be maintained at 71% within a relatively low cell-concentration range (50 cells per milliliter). In addition, under weakly acidic conditions, Ni-C@MOF-Apt exhibits peroxidase (POD) and oxidase (OXD) activities, which can generate reactive oxygen species (ROS) to eliminate MCF-7 cells in synergy with photodynamic effects. This strategy combines nanozyme activity with photodynamic effects and improves the killing efficacy against MCF-7 cells.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149035232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Portable single-step ethanolamine-functionalized boron-doped diamond immunosensor for dual detection of CEA and CYFRA 21–1 用于CEA和CYFRA 21-1双重检测的便携式单步乙醇胺功能化掺杂硼金刚石免疫传感器

IF 5.4 2区化学

Microchimica Acta Pub Date : 2026-09-25 DOI: 10.1007/s00604-026-08396-7

Sowmya Joshi, N. Arunachalam, M S Ramachandra Rao, Swathi Sudhakar

{"title":"Portable single-step ethanolamine-functionalized boron-doped diamond immunosensor for dual detection of CEA and CYFRA 21–1","authors":"Sowmya Joshi, N. Arunachalam, M S Ramachandra Rao, Swathi Sudhakar","doi":"10.1007/s00604-026-08396-7","DOIUrl":"10.1007/s00604-026-08396-7","url":null,"abstract":"<div><p>A single-step ethanolamine-functionalized boron-doped diamond (BDD) immunosensor for the dual detection of carcinoembryonic antigen (CEA) and cytokeratin fragment 21–1 (CYFRA 21–1). Unlike conventional multi-step carbodiimide-based coupling strategies, the ethanolamine (ETA) modification provides a simplified amine-terminated surface for efficient antibody immobilization. Comprehensive characterization confirmed successful layer-by-layer assembly: scanning electron microscopy (SEM) revealed surface morphological evolution, while X-ray photoelectron spectroscopy (XPS), Contact angle measurements and attenuated total reflectance Fourier-transform infrared spectroscopy (ATR-FTIR) verified amine group integration and biomarker attachment. The platform showed concentration-dependent current responses across the range of 0.001–400 ng/mL and achieved statistically calculated limits of detection of 0.085 pg/mL for CEA and 0.043 pg/mL for CYFRA 21–1. The sensor exhibited good selectivity, reproducibility, stability, and reliable performance in spiked human serum samples with minimal matrix interference. In addition, an Arduino-based portable readout closely matched bench-top measurements, supporting the potential of the platform for point-of-care lung cancer screening.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149035163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fe3O4–Au cluster–satellite particles as direct colorimetric reporters in a smartphone-quantified lateral flow immunoassay for prostate-specific antigen Fe3O4-Au簇卫星颗粒在智能手机定量前列腺特异性抗原的侧流免疫分析中作为直接比色报告

IF 5.4 2区化学

Microchimica Acta Pub Date : 2026-09-25 DOI: 10.1007/s00604-026-08422-8

Daniele Marra, Erica Cavaliere, Daniela Terracciano, Luca Livio Levorato, Tina D’Aponte, Vincenzo Iannotti, Francesco Gentile, Raffaele Velotta, Bartolomeo Della Ventura

{"title":"Fe3O4–Au cluster–satellite particles as direct colorimetric reporters in a smartphone-quantified lateral flow immunoassay for prostate-specific antigen","authors":"Daniele Marra, Erica Cavaliere, Daniela Terracciano, Luca Livio Levorato, Tina D’Aponte, Vincenzo Iannotti, Francesco Gentile, Raffaele Velotta, Bartolomeo Della Ventura","doi":"10.1007/s00604-026-08422-8","DOIUrl":"10.1007/s00604-026-08422-8","url":null,"abstract":"<div><p>Fe₃O₄–Au cluster–satellite magnetic particles (CSMPs) were investigated as high-payload direct colorimetric reporters in a smartphone-quantified sandwich LFA for prostate-specific antigen (PSA) determination in undiluted human serum. Commercially available PEGylated dextran-coated Fe₃O₄ magnetic clusters were used as scaffolds, decorated with multiple gold nanoparticles, and subsequently functionalized with anti-PSA antibodies through photochemical immobilization. Magnetic recoverability facilitated particle isolation during synthesis and bioconjugation, whereas assay transduction remained exclusively optical, without magnetic actuation, external excitation, or post-assay signal amplification. Systematic variation of reporter extinction revealed a non-monotonic relationship between particle loading and analytical performance. Low particle loading produced weak optical signals, whereas excessive loading caused early saturation and compression of the test-to-control response. An intermediate extinction provided the best balance between signal intensity, low-concentration detectability, and usable dynamic response. Under optimized conditions, the assay provided an estimated limit of detection of 0.25 ng·mL⁻¹ within 20 min. The assay showed limited interference from human kallikrein 2, intra- and inter-assay coefficients of variation below 10%, and preliminary agreement with a reference chemiluminescent immunoassay in seven human serum samples. These results demonstrate that CSMPs can serve as direct high-contrast colorimetric reporters for quantitative LFA while preserving a simple smartphone-based readout.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-026-08422-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149035233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Cryodesiccation-driven crystallization preparation approach for zinc(II)-phthalocyanine nanodots in cancer photodynamic therapy and photoacoustic imaging 修正：低温干燥驱动的锌(II)-酞菁纳米点在癌症光动力治疗和光声成像中的结晶制备方法。

IF 5.4 2区化学

Microchimica Acta Pub Date : 2026-09-22 DOI: 10.1007/s00604-026-08411-x

Hui Ding, Yanjuan Cai, Jianxiong Chen, Tong Lu, Weiping Wen, Guohui Nie, Xiaojun Wang

引用次数: 0

Photoactivated fluorescent nanozymes from mulberry leaf-derived carbon dots for dual-mode antioxidant determination and biothiol contribution estimation 桑叶碳点光激活荧光纳米酶用于双模式抗氧化测定和生物硫醇贡献估算。

IF 5.4 2区化学

Microchimica Acta Pub Date : 2026-09-21 DOI: 10.1007/s00604-026-08407-7

Shijie Li, Qiongmeng Lu, Xiling Fang, Qiuju He, Xing Tong, Ze Chen, Lin Han

{"title":"Photoactivated fluorescent nanozymes from mulberry leaf-derived carbon dots for dual-mode antioxidant determination and biothiol contribution estimation","authors":"Shijie Li, Qiongmeng Lu, Xiling Fang, Qiuju He, Xing Tong, Ze Chen, Lin Han","doi":"10.1007/s00604-026-08407-7","DOIUrl":"10.1007/s00604-026-08407-7","url":null,"abstract":"<div><p>Contribution-resolved antioxidant determination in complex food matrices remains challenging because the total antioxidant response usually arises from multiple overlapping reducing species. Herein, red-emissive carbon dots (CDs) were prepared from mulberry leaves via a solvothermal method and developed as highly efficient photoactivated fluorescent nanozymes for dual-mode antioxidant determination. The CDs exhibited a large Stokes shift (Δλ = 272 nm) and were stably dispersed in aqueous media with the assistance of Tween-20. Under 365 nm irradiation, the CDs showed oxidase-like activity toward 3,3′,5,5′-tetramethylbenzidine (TMB), producing oxidized TMB (oxTMB) and simultaneously inducing fluorescence quenching through an inner-filter-effect-dominated process. In the presence of antioxidants, oxTMB was reduced, accompanied by color fading and fluorescence recovery, thereby establishing a light-gated “oxidation–quenching/reduction–recovery” dual-mode sensing platform. Using biothiols as model reductive analytes, the limits of detection for Cys and GSH were 0.29 and 0.16 µM in the colorimetric mode, and 0.34 and 0.26 µM in the fluorescent mode, respectively. More importantly, by integrating N-ethylmaleimide-mediated thiol blocking with total antioxidant measurement, the platform enabled differential estimation of the biothiol-associated reducing contribution in complex soy sauce samples. The total antioxidant capacity was determined to be 1392.3–2643.5 µM Cys-equivalent, while the biothiol-associated contribution was estimated to be 102.4–384.8 µM Cys-equivalent. This work provides a light-gated dual-mode nanozyme platform and a chemically selective strategy for contribution-resolved antioxidant determination in complex fermented food matrices.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a graphene oxide-based PEGylated fluorescent aptasensor with ultrahigh-sensitivity and anti-fouling properties for the detection of serum carcinoembryonic antigen levels 一种具有超高灵敏度和抗污染特性的氧化石墨烯基聚乙二醇荧光配体传感器的研制，用于检测血清癌胚抗原水平。

IF 5.4 2区化学

Microchimica Acta Pub Date : 2026-09-21 DOI: 10.1007/s00604-026-08412-w

Qi Feng, Lu Xu, Ding-Fan Guo, Qi Wen, Yun-Hui Liang, Qi Huang, Ting Wang, Kun-He Zhang

{"title":"Development of a graphene oxide-based PEGylated fluorescent aptasensor with ultrahigh-sensitivity and anti-fouling properties for the detection of serum carcinoembryonic antigen levels","authors":"Qi Feng, Lu Xu, Ding-Fan Guo, Qi Wen, Yun-Hui Liang, Qi Huang, Ting Wang, Kun-He Zhang","doi":"10.1007/s00604-026-08412-w","DOIUrl":"10.1007/s00604-026-08412-w","url":null,"abstract":"<div><p>The specificity and sensitivity of aptamer-based serum biomarker assays can be compromised by nonspecific adsorption of proteins. In this study, we used the carcinoembryonic antigen (CEA) aptamer as a model to develop a fluorescent aptasensor capable of specifically and sensitively detecting serum biomarkers. This aptasensor employs the good antifouling capability of polyethylene glycol 2000 (P2K) to enhance specificity and the good fluorescence resonance energy transfer capability of graphene oxide (GO) to improve sensitivity. The aptasensor was applied to determine pure CEA protein and CEA in clinical serum and demonstrated that the P2K-modified fluorescent aptasensor exhibited significantly superior performance in CEA detection compared with the unmodified aptasensor, including accuracy, precision, linear range, antifouling properties, and selectivity. In the detection of CEA levels in clinical serum samples, the P2K-modified fluorescent aptasensor exhibited a lower limit of detection (LOD) (1.31 vs. 1.90 ng/mL), a wider linear range (1–256 vs. 1–64 ng/mL), better recovery (92.4%–103.6% vs. 73.3%–124.1%), and a smaller relative standard deviation (RSD) (0.7%-2.5% vs. 2.0%-6.4%). The aptasensor is capable of determining CEA levels within 90 min using only 1 µL of serum, with advantages of requiring no prefabrication of sensors, measuring fluorescence intensity in a conventional microplate reader, operating experimentally at room temperature, and high-throughput detection. Conclusively, this study successfully developed a GO-based P2K-modified fluorescent aptasensor capable of specifically, sensitively, and conveniently detecting CEA levels in clinical serum samples and with potential for clinical application.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

D2O metabolic labeling combined with AuNPs@PVP/SA hydrogel SERS platform for rapid quantitative detection of active lactic acid bacteria D2O代谢标记结合AuNPs@PVP/SA水凝胶SERS平台快速定量检测活性乳酸菌。

IF 5.4 2区化学

Microchimica Acta Pub Date : 2026-09-21 DOI: 10.1007/s00604-026-08404-w

Shijie Liu, Lijun Zhao, Yueyu Bai, Qian Ding, Yangyang Ma, Miaoyun Li, Dong Liang, Yanxia Liu, Haoyun Li, Lingxia Sun

{"title":"D2O metabolic labeling combined with AuNPs@PVP/SA hydrogel SERS platform for rapid quantitative detection of active lactic acid bacteria","authors":"Shijie Liu, Lijun Zhao, Yueyu Bai, Qian Ding, Yangyang Ma, Miaoyun Li, Dong Liang, Yanxia Liu, Haoyun Li, Lingxia Sun","doi":"10.1007/s00604-026-08404-w","DOIUrl":"10.1007/s00604-026-08404-w","url":null,"abstract":"<div><p>Achieving accurate quantification and counting of active lactic acid bacteria (LAB) is an important technical guarantee for the industrial production and application of LAB and their products. In this study, a gold nanoparticles@polyvinylpyrrolidone/sodium alginate (AuNPs@PVP/SA) hydrogel SERS sensor with outstanding stability and sensitivity was developed. Utilizing this platform as a substrate material to mitigate the “coffee ring” effect, we explored a strategy for the precise quantitative determination of mixed active LAB based on the AuNPs@PVP/SA hydrogel SERS platform and D<sub>2</sub>O-probed Raman spectroscopy technology. The results indicated that the SERS platform based on AuNPs@PVP/SA hydrogel exhibited excellent spectral reproducibility for LAB as a SERS substrate material, with a significantly reduced “coffee ring” effect. This strategy enabled rapid and quantitative detection of active lactic acid bacteria (LAB), with a limit of detection (LOD) of 10 CFU/mL. Moreover, the strategy had excellent analytical capabilities for the qualitative identification and precise quantification of different species of active LAB in actual samples, achieving an average accuracy of 98.67% with <i>RSD</i> values all less than 5%. It provides a potential technical support for the dynamic monitoring of relative abundance and metabolic phenomena of mixed active LAB in co-culture systems during actual industrial production.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149015170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Water-induced supramolecular self-assembly of porphyrin integrated with multivalent metal–organic framework for enhanced electrochemiluminescence and ultrasensitive aptasensing of 4-fluoromethamphetamine 水诱导卟啉与多价金属-有机骨架的超分子自组装增强4-氟甲基安非他明的电化学发光和超灵敏的适体感应

IF 5.4 2区化学

Microchimica Acta Pub Date : 2026-09-18 DOI: 10.1007/s00604-026-08418-4

Bingxue Fan, Ding Jiang, Yuan Ni, Tengfei Meng, Zheng Sun, Haibo Li, Wenchang Wang, Zhidong Chen

{"title":"Water-induced supramolecular self-assembly of porphyrin integrated with multivalent metal–organic framework for enhanced electrochemiluminescence and ultrasensitive aptasensing of 4-fluoromethamphetamine","authors":"Bingxue Fan, Ding Jiang, Yuan Ni, Tengfei Meng, Zheng Sun, Haibo Li, Wenchang Wang, Zhidong Chen","doi":"10.1007/s00604-026-08418-4","DOIUrl":"10.1007/s00604-026-08418-4","url":null,"abstract":"<div><p>Aggregation-caused quenching (ACQ) and poor aqueous solubility severely restrict electrochemiluminescence (ECL) applications of polycyclic aromatic hydrocarbons (PAHs). Herein, a water-induced supramolecular self-assembly strategy is proposed to address these drawbacks. Via a simple reprecipitation route, representative PAH meso-tetrakis(4-carboxyphenyl)porphyrin (TCPP) forms ordered supramolecular aggregates driven by noncovalent interactions, which suppress π–π stacking and ACQ and achieve a low triggering potential of − 1.0 V. Iron-based MOF supported molybdenum disulfide (MIL-101@MoS<sub>2</sub>) is adopted as a multifunctional amplifier: reversible valence interconversion of Fe<sup>2+</sup>/Fe<sup>3+</sup> and Mo<sup>4+</sup>/Mo<sup>6+</sup> sustains continuous redox cycling to improve redox activity, while abundant oxygen vacancies accelerate electron transfer and coreactant activation for further ECL enhancement. After preparing MIL-101(Fe)@MoS<sub>2</sub>@TCPP nanoparticles (MMST), an ECL aptasensor is fabricated for 4-fluoromethamphetamine (4-FMA) determination relying on target-triggered cyanine dye displacement. This sensor exhibits an ultralow limit of detection of (1.87 × 10<sup>− 15</sup> g/L) (S/<i>N</i> = 3) and a broad linear range from (1.0 × 10<sup>− 14</sup> – 1.0 × 10<sup>− 6</sup> g/L) g/L. It also shows favorable analytical results for real e-cigarette samples, with recoveries of 95.0–104.0% and relative standard deviations (RSD) less than 5.0%. This work offers a green route for high-performance PAH-based ECL luminophores and a reliable tool for rapid screening of emerging psychoactive contaminants in complicated food-related matrices.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148986796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A chiral silicon-based electrochemical sensor for the detection of L-glutamate 一种检测l -谷氨酸的手性硅基电化学传感器

IF 5.4 2区化学

Microchimica Acta Pub Date : 2026-09-18 DOI: 10.1007/s00604-026-08415-7

Ziyao Qin, Menggai Jia, Yue Zhan, Molin Yang, Beibei Hu, Zhiwei Li, Shiguo Sun

{"title":"A chiral silicon-based electrochemical sensor for the detection of L-glutamate","authors":"Ziyao Qin, Menggai Jia, Yue Zhan, Molin Yang, Beibei Hu, Zhiwei Li, Shiguo Sun","doi":"10.1007/s00604-026-08415-7","DOIUrl":"10.1007/s00604-026-08415-7","url":null,"abstract":"<div><p><i>L</i>-Glutamate (<i>L</i>-Glu), a major excitatory neurotransmitter in the central nervous system, plays an important role in nitrogen metabolism. In this study, chiral mesoporous imprinted silica nanoparticles (CMISNs) were synthesized via the sol-gel method using <i>L</i>-Glu derivatives as templates. Subsequently, the AuNPs/CMISNs@CMK-3/GCE electrochemical sensing interface was constructed. Benefiting from the chiral mesoporous structure of CMISNs and combined high conductivity of CMK-3 and signal amplification effect of gold nanoparticles (AuNPs), the designed electrochemical sensor enables specific detection of <i>L</i>-Glu. The developed sensor exhibits a wide linear response range for <i>L</i>-Glu (1 nM to 1 mM), with a limit of detection of 0.15 nM and a limit of quantification of 0.52 nM. It also demonstrated remarkable enantioselectivity, achieving a chiral separation factor of 7.7 toward glutamate enantiomers, along with good reproducibility (RSD = 3.2%, <i>n</i> = 5). The sensor retained excellent stability over 14 days and high selectivity against potential interferents. In contrast to conventional enzyme-based biosensors, chiral recognition is achieved by imprinted mesopores of <i>L</i>-Glu without the involvement of biological enzymes. This work provides a novel bionic sensing platform for the precise detection of <i>L</i>-Glu, which holds significant potential in neuroscience research and clinical diagnosis. </p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148986916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel carbon black-integrated cage-structured NiCo2O4 nanocomposite for the selective and sensitive electrochemical determination of vanillin 一种新型炭黑集成笼状NiCo2O4纳米复合材料，用于电化学测定香兰素的选择性和敏感性

IF 5.4 2区化学

Microchimica Acta Pub Date : 2026-09-18 DOI: 10.1007/s00604-026-08405-9

Li Zhang, Shuiying Xiong, Liting Jiang, Jiaxin Qiu, Qian Jin, Xinding Lv, Xinman Tu

{"title":"A novel carbon black-integrated cage-structured NiCo2O4 nanocomposite for the selective and sensitive electrochemical determination of vanillin","authors":"Li Zhang, Shuiying Xiong, Liting Jiang, Jiaxin Qiu, Qian Jin, Xinding Lv, Xinman Tu","doi":"10.1007/s00604-026-08405-9","DOIUrl":"10.1007/s00604-026-08405-9","url":null,"abstract":"<div><p>A novel electrochemical sensor for determination of vanillin based on carbon black and NiCo<sub>2</sub>O<sub>4</sub> nanocomposite (CB@NiCo<sub>2</sub>O<sub>4</sub>) was established. The nanocomposite was immobilized onto electrode surface, the carbon black can selectively adsorb vanillin via π-π stacking interactions between the aromatic domains and the benzene ring of vanillin. Concurrently, the NiCo<sub>2</sub>O<sub>4</sub> with cage-structure enhances the electrochemical signal response of the adsorbed vanillin by the high specific surface area and favorable ion-exchange capability. Under optimized experimental conditions, the sensor exhibits a linear range of 0.1 ~ 60 µM with a limit of detection of 30 nM, and the selectivity and signal stability were confirmed to be satisfactory. In real-sample analysis, the results are agreement with the contrast testing results of high-performance liquid chromatography, and the spike recovery experiment values ranged from 95.35% to 103.80%, with relative standard deviations (RSDs) of 3.77%~4.59% (<i>n</i> = 3). The proposed electrochemical sensor possesses a favorable application prospect in product quality control.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 10","pages":""},"PeriodicalIF":5.4,"publicationDate":"2026-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148986914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0