Microchimica Acta最新文献

{"title":"A ratiometric electrochemical sensor based on Cu@Ni/MWCNTs for detection of chloramphenicol","authors":"Xiaoxue Liu,&nbsp;Dongyang Wang,&nbsp;Rongxuan Xu,&nbsp;Xing Gao,&nbsp;Mingyang Han,&nbsp;Yurong Guo,&nbsp;Lei Yu","doi":"10.1007/s00604-025-07091-3","DOIUrl":"10.1007/s00604-025-07091-3","url":null,"abstract":"<div><p>A signal tag was successfully designed by means of two-step reduction approach, in which CuNi nanoparticles (CuNi NPs) uniformly distributed on the surface of multiwall carbon nanotubes (MWCNTs). This composites not only inherits excellent conductivity and surface area of MWCNTs, but also endows the material with superior electrocatalytic performance due to the introduction of CuNi NPs. Then, a ratiometric sensing platform coupled with built-in correction ability for convenient direct determination of chloramphenicol (CAP) was exploited, wherein Cu@Ni/MWCNTs were used as signal label and ferrocene (Fc) as internal reference. It is noteworthy that ratiometric measurement was performed by directly incorporating Fc into the electrolyte solution. The profound investigation into the sensing performance of the implemented sensor revealed that Cu@Ni/MWCNTs nanocomposites exhibit satisfactory electrocatalytic activity and stability. Additionally, the integration of the ratiometric strategy markedly enhanced the reliability and repeatability and exhibited decent performance in CAP determination varying from 0.1 to 10 μM. Overall, the corporation of Cu@Ni/MWCNTs with excellent electrocatalytic ability as well as elaborated ratiometric method makes it a valuable tool for future assaying an extensive range of substances.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction and application of magnetic surface molecularly imprinted solid-phase extraction for the detection of 5-hydroxytryptamine in peripheral blood","authors":"Gege Zhu,&nbsp;Xinyu Li,&nbsp;Qianjie Ma,&nbsp;Zhanying Hong,&nbsp;Dan Zhong,&nbsp;Xiaodong Sun","doi":"10.1007/s00604-025-07058-4","DOIUrl":"10.1007/s00604-025-07058-4","url":null,"abstract":"<div><p>The 5-Hydroxytryptamine (5-HT) level determination is crucial for predicting, pathogenesis, diagnosis, and pharmacological treatment of schizophrenia. To realize the extraction of trace 5-HT in complex matrix samples, a magnetic molecularly imprinted solid-phase extraction (MMISPE) pretreatment method was developed. In combination with UPLC-MS/MS, the method was possible to achieve the quantification of 1 ng/mL 5-HT in tissue samples. The type of adsorbent, magnetic surface molecularly imprinted polymers (MMIPs) adsorbent dosage, adsorption temperature, and adsorption method were screened to obtain the better extraction of 5-HT. After optimizing the extraction and separation method, we applied the MMISPE method to the detection of 5-HT in peripheral blood of clinical schizophrenia patients. The results showed that the average concentration of 5-HT in the peripheral blood of healthy controls was 74.30 ng/mL, whereas the 5-HT content in the samples from schizophrenic patients was 1.17 ng/mL.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sulfonated reduced graphene oxide-doped polypyrrole film prepared by in situ electropolymerization and coating in fabrication of ammonia gas sensor for exhaled breath analysis of patients with kidney failure","authors":"Fatemeh Ferdosi,&nbsp;Mahsa Amiri,&nbsp;Naader Alizadeh","doi":"10.1007/s00604-025-07075-3","DOIUrl":"10.1007/s00604-025-07075-3","url":null,"abstract":"<div><p>Detection of the level of ammonia gas in exhaled breath provides non-invasive and fast diagnosis of kidney failure. Here, we fabricated room temperature and sensitive chemiresistive ammonia gas sensor by in situ electropolymerization and deposition of polypyrrole/sulfonated graphene oxide (PPy/SRGO) on/between gold interdigitated electrodes (Au-IDEs). The prepared sensors were characterized by using field emission scanning electron microscopy (FESEM) and Fourier transform infrared (FT-IR). The fabricated ammonia gas sensor was tested at different operating temperatures (26–50°C) and we selected room temperature for simplifying operation (26°C). At this temperature, the sensor showed two linear ranges of 5–40 ppb (R² = 0.99) and 40–5000 ppb (R² = 0.98) with the detection limit of 2.7 ppb. The fabricated gas sensor showed good selectivity toward ammonia in comparison with different interfering gases like acetone, dibutylamine, ethanol, methanol, and humid air (RH = 86%). According to the exhaled breath analysis, the fabricated sensor can determine ammonia level in the patient with kidney failure compared with the healthy persons. The results are with a good linear correlation to the clinical blood test. So this study presents the facile, rapid, and sensitive measurement of ammonia gas in human exhaled breath as a non-invasive diagnosis of kidney disease.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MoS₂-DNA tetrahedral bioconjugate for high-performance DNA biosensors: application in viral infection diagnostics","authors":"Estefanía Enebral-Romero,&nbsp;Emiliano Martínez-Periñán,&nbsp;David López-Diego,&nbsp;Mónica Luna,&nbsp;Marina Garrido,&nbsp;Cristina Navío,&nbsp;Emilio M. Pérez,&nbsp;Encarnación Lorenzo,&nbsp;Tania García-Mendiola","doi":"10.1007/s00604-025-07084-2","DOIUrl":"10.1007/s00604-025-07084-2","url":null,"abstract":"<div><p> An electrochemical DNA biosensor is presented for early viral infection detection, integrating molybdenum disulphide (MoS₂), tetrahedral DNA nanostructures (TDNs), and thionine-modified carbon nanodots (CNDsTy). The innovation of this work lies in the first-time integration of these nanomaterials for the preparation of a bioconjugate, whose synergy enables the biosensor’s functionality. MoS₂ anchors the TDNs, which carry the capture probe for virus identification via genetic code recognition. CNDsTy allow the electrochemical detection based on their different affinity for single-stranded (ssDNA) and double-stranded DNA (dsDNA), enabling hybridization event identification. The biosensor achieves high sensitivity (detection limit of 5.00 fM) and can distinguish viral loads, validated with the SARS-CoV-2 ORF1ab sequence in human nasopharyngeal samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07084-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143594782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering fluorescent carbon dot sensor with rare earth europium for the detection of uranium (VI) ion in vivo","authors":"Xiayu Zhou,&nbsp;Yue Wang,&nbsp;Jiayi Song,&nbsp;Lihao Xiong,&nbsp;Xin Zhao,&nbsp;Sihan Chen,&nbsp;Weichao Zhao,&nbsp;Le Li,&nbsp;Deshuai Zhen","doi":"10.1007/s00604-025-07078-0","DOIUrl":"10.1007/s00604-025-07078-0","url":null,"abstract":"<div><p>Three types of carbon dots were synthesized using the same precursor (folic acid and europium nitrate) via different preparation methods (doping and direct coordination). A comprehensive comparison and analysis of the morphology, surface groups, and optical properties of the prepared carbon dots (CD), europium-doped carbon dots (CD-Eu), and europium-functionalized carbon dots (CD@Eu) were conducted. Moreover, due to the higher quantum yield, excellent stability, and outstanding selectivity for UO₂²⁺ exhibited by CD-Eu, we selected CD-Eu as the probe for subsequent applications. CD-Eu showed a sensitive response to UO₂²⁺ within the concentration range 25 ~ 200 nM, with a detection limit of 0.84 nM (0.42 μg·L⁻¹). Additionally, CD-Eu demonstrated excellent accuracy and recovery in spiked detection of real water samples. Furthermore, we discovered that this probe could detect UO₂²⁺ both <i>in</i> <i>vitro</i> and <i>in</i> <i>viv</i>o. This strategy provides a promising fluorescent sensor for the detection of UO₂²⁺ in water and biological samples, holding significant implications for addressing UO₂²⁺ contamination issues.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A label-free colorimetric assay for sensitive screening of T4 polynucleotide kinase activity and inhibition based on enzyme-aided cyclic strand displacement amplification and PNA-gold nanoparticle platform","authors":"Xiaojun Yuan,&nbsp;Long Chen,&nbsp;Yang Zhao,&nbsp;Li Cheng,&nbsp;Chao Zhao","doi":"10.1007/s00604-025-07086-0","DOIUrl":"10.1007/s00604-025-07086-0","url":null,"abstract":"<div><p>The phosphorylation of nucleic acids mediated by 5′-polynucleotide kinase (PNK) exerts a crucial regulatory function in a wide range of significant cellular activities. Nevertheless, the current approaches for detecting PNK require expensive labeled probes and complex instrumentation, making it impossible to achieve real-time, on-site, and rapid analysis. Here, we take T4 PNK as a model and establish a novel colorimetric strategy for the detection of PNK activity and its inhibition by means of a coupled enzyme-assisted cyclic strand displacement amplification (SDA) and peptide nucleic acid (PNA)-gold nanoparticle (AuNP) based platform. The inspiration for this innovative strategy comes from the high stability, strong binding ability, and potent regulatory effect of PNA probes on AuNPs. Under the catalysis of PNK, the 5′-hydroxyl end of the hairpin-shaped DNA (hpDNA) is initially phosphorylated and subsequently digested by λ exonuclease (λ exo). This results in the release of a single-stranded DNA, which serves as a triggering factor to initiate the strand displacement reaction (SDR). The replaced PNA probe adheres to the surface of AuNPs, inducing their aggregation and causing a remarkable color change. Meanwhile, the double-stranded SDR product releases the SDR trigger with the aid of a nicking enzyme, triggering the next round of the SDR cycle and achieving highly efficient and controllable signal amplification. This assay is simple to operate and does not require bulky and expensive instruments or complex labeled probes. Compared with existing colorimetric methods, the detection sensitivity has been greatly improved, reaching 3.52 × 10⁻⁴ U/mL. Additionally, the method has demonstrated satisfactory results when applied to intricate biological matrices and the screening of T4 PNK inhibitors. Therefore, the proposed strategy holds significant potential for real-time analysis, high-throughput detection, and PNK-related drug screening.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07086-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of trypsin-protected CsPbCl3 fluorescent nanocrystals for hydroxyl radical sensing","authors":"Suresh Kumar Kailasa,&nbsp;Kartik Pankajbhai Makwana,&nbsp;Madhura Pradeep Deshpande,&nbsp;Yoojin Choi,&nbsp;Ruth Stephanie,&nbsp;Chan Yeong Park,&nbsp;Tae Jung Park","doi":"10.1007/s00604-025-07070-8","DOIUrl":"10.1007/s00604-025-07070-8","url":null,"abstract":"<div><p>Water-dispersible perovskite nanocrystals (PNCs) show promising applications in recognizing ionic and molecular species because of their excellent optical properties. However, lead halide PNCs have some limitations when they are used as probes for molecular species sensing in aqueous media. Here, we introduce trypsin (Try) as a bioligand for the synthesis of cesium lead chloride (CsPbCl₃) PNCs with high water stability. The as-fabricated Try-CsPbCl₃ PNCs show λ_Em/Ex at 433/370 nm with a quantum yield of 17.26%. The fluorescence emission spectral characteristics of Try-CsPbCl₃ PNCs demonstrated that water-stable Try-CsPbCl₃ PNCs acted as a promising fluorescent probe for the detection of hydroxyl radical (^•OH) via turn-off mechanism. The Try-CsPbCl₃ PNCs-based turn-off fluorescence approach displayed good selectivity for hydroxyl radical in water, showing a wider linear range (0.01–5 µM) with a remarkable detection limit of 3.10 nM for hydroxyl radical. The Try-CsPbCl₃ PNCs were demonstrated to be a facile probe for sensing ^•OH in water samples, which signifies that Try-CsPbCl₃ PNCs exhibited broad applications for hydroxyl radical sensing in real samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07070-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioaccessibility and cellular transport study of silver and titanium dioxide nanoparticles from exposed seaweed and mussels using Caco-2 cells","authors":"Juan José López-Mayán,&nbsp;Raquel Domínguez-González,&nbsp;María Carmen Barciela-Alonso,&nbsp;Elena Peña-Vázquez,&nbsp;Antonio Moreda-Piñeiro,&nbsp;Pablo Taboada-Antelo,&nbsp;Pilar Bermejo-Barrera","doi":"10.1007/s00604-025-07066-4","DOIUrl":"10.1007/s00604-025-07066-4","url":null,"abstract":"<div><p>Bioavailability studies on pollution pre-concentrator organisms such as algae and mussels are necessary to ensure food safety, particularly in the case of nanomaterials whose industrial applications have increased in recent years. Thus, the bioaccessibility and the bioavailability of total Ag and Ti and AgNPs and TiO₂NPs from raw and cooked seaweed (<i>Palmaria palmata</i> and <i>Ulva</i> sp.) and cooked mussels (<i>Mytilus edulis</i>) exposed to 1.0 mg L⁻¹ of 15 nm PVP-AgNPs, 1.0 mg L⁻¹ of 25 nm citrate-TiO₂NPs, or 1.0 mg L⁻¹ 5 nm citrate-TiO₂NPs, for 28 days, were evaluated. Samples were subjected to an in vitro process simulating human gastrointestinal digestion while cell transport from the intestinal lumen to the bloodstream was mimicked using Caco-2 cell cultures. Total Ag and Ti in the digest samples, the bioaccessible fractions, the apical, and basolateral fractions were determined by ICP-MS, while AgNPs and TiO₂NPs were determined by single-particle-ICP-MS (SP-ICP-MS). Finally, the presence of nanoparticles in the Caco-2 cells was verified by single-cell-ICP-MS (SC-ICP-MS). AgNP bioaccessibility ratios were between 22% and 97% for seaweed and 18% for mussels, while for TiO₂NPs were between 17% and 81% (seaweed) and 76% and 100% (mussels). Nanoparticle cellular transports were in all cases less than 1%. However, the mass percentages of Ag as NPs and Ti as NPs in the Caco-2 cells for raw and cooked seaweed were 9% and 7% and 20% and 6%, respectively. These results confirm a small transport of the nanoparticles through the Caco-2 cells under the proposed experimental conditions.\u0000</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07066-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent aptasensor for detection of Salmonella typhimurium using boric acid-functionalized terbium metal–organic framework and magnetic nanoparticles","authors":"Dinesh Kumar,&nbsp;Harpreet Singh,&nbsp;Simran Makkar,&nbsp;Nitin Singhal,&nbsp;Akash Deep,&nbsp;Sanjeev Soni","doi":"10.1007/s00604-025-07073-5","DOIUrl":"10.1007/s00604-025-07073-5","url":null,"abstract":"<div><p>A fluorescent detection platform was designed using boric acid-functionalized terbium metal–organic framework (BA-Tb-MOF) and carboxyl-modified magnetic nanoparticles (MNPs) to identify <i>Salmonella typhimurium </i>(<i>S. typhimurium</i>) bacteria. Firstly, carboxyl-modified Fe₃O₄MNPs were coated with specific aptamer (Apt-MNPs) as the capture probe for <i>S. typhimurium</i>. Then, the Apt-MNPs were added to the bacterial suspension to facilitate the targeted binding. Subsequently, the fluorescent probe (BA-Tb-MOF) was introduced into this solution. The BA-Tb-MOF was strongly attached to the bacterial surface through interactions between BA and glycolipids on the bacterial cell walls, forming a stable complex. As the bacterial concentration increased, the fluorescence intensity of the solution progressively decreased due to the binding and removal of bacteria-Apt-MNPs/BA-Tb-MOF complexes through magnetic separation. Under optimum conditions, the concentration of <i>S. typhimurium</i> and the fluorescence intensity showed an inverse linear relationship within the range of 10¹–10⁹ CFU/mL, and the detection limit was 4 CFU/mL. The developed sensor showed high specificity against several other pathogenic bacteria such as <i>E. coli</i>, <i>S. aureus</i>, and <i>P. aeruginosa</i>. The developed fluorescence platform also successfully detected the <i>S. typhimurium</i> in drinking water and egg samples with satisfactory recoveries (83–98%). This strategy can be investigated further for the detection of <i>S. typhimurium</i> and other pathogens in food and clinical samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive detection of doxycycline enabled by oxygen vacancy modulated TiO2 nanotubes","authors":"Juan Gao,&nbsp;Sen Yang,&nbsp;Chen Xu,&nbsp;Zerui Dong,&nbsp;SiZhu Chen,&nbsp;Lingcheng Zheng,&nbsp;Leilei Lan,&nbsp;Gang He","doi":"10.1007/s00604-025-07072-6","DOIUrl":"10.1007/s00604-025-07072-6","url":null,"abstract":"<div><p>TiO₂ nanotubes rich in oxygen vacancies (Ov), which were successfully fabricated on Ti foils, were used as the working electrode of a photoelectrochemical (PEC) sensor. The TiO₂ nanotube electrode optimized with abundant Ov demonstrated a remarkable photocurrent density of 1.03 mA/cm², which is approximately 2.9 times higher than that of the TiO₂ nanotube electrode. When applied to the detection of DOC, this electrode exhibited a wide linear detection range spanning from 0.1 to 100 μM and achieved an exceptionally low detection limit of 0.043 μM with a signal-to-noise ratio of 3. Furthermore, comparative experiments indicated that the Ov-enriched TiO₂ nanotube electrode exhibited excellent anti-interference capabilities and long-term stability, ensuring the accuracy and reliability of the detection outcomes. The superior detection performance is primarily attributed to two aspects: on one hand, Ov act as electron traps, facilitating the capture and transfer of photogenerated electrons, effectively prolonging the lifetime of these carriers; on the other hand, Ov also serves as active sites, enhancing the adsorption of DOC molecules and reaction kinetics, further amplifying the detection signal. This work offers a theoretical and experimental groundwork for the rapid monitoring of residual antibiotics.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div><div><p>Graphical Abstract</p></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}