A smartphone-integrated portable colorimetric sensing platform for ascorbic acid based on a PtAu@Rh nanozyme with high specific activity

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-09-16 DOI:10.1007/s00604-025-07530-1

Jian Hao, Xiaojie Dong, Zeyang Yu, Kai Cai

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Abstract

A PtAu@Rh nanozyme with strong peroxidase (POD)-like activity was synthesized via the template method, and a colorimetric sensing platform for ascorbic acid (AA) detection based on UV‒vis or smartphone assistance was established. The results revealed that the specific activity of the PtAu@Rh-2 nanozyme reached 1789 U mg⁻¹, with V_max and K_m of 1.3488 × 10⁻⁶ M s⁻¹ and 1.8286 mM, respectively. Under optimal detection conditions, the developed smartphone-assisted method achieved a wide detection range (0.5–50 μM) and a low limit of detection (LOD, 0.138 μM). Spike recovery experiments in various actual samples verified the reliability and feasibility of the developed method, yielding recoveries of 95.34% to 104.28% and relative standard deviations of 0.29% to 5.06%, both within the acceptable standard range. This work provides not only a reliable strategy for synthesizing high-efficiency nanozymes but also a convenient AA detection method to help individuals make reasonable and healthy dietary choices.

Graphical Abstract

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基于高比活性PtAu@Rh纳米酶的智能手机集成便携式抗坏血酸比色传感平台

采用模板法合成了具有较强过氧化物酶（POD）样活性的PtAu@Rh纳米酶，并建立了基于UV-vis或智能手机辅助的抗坏血酸（AA）比色检测平台。结果表明，PtAu@Rh-2纳米酶的比活性达到1789 U mg−1，Vmax和Km分别为1.3488 × 10−6 M s−1和1.8286 mM。在最佳检测条件下，该方法具有较宽的检测范围（0.5 ~ 50 μM）和较低的检测限（LOD为0.138 μM）。在各种实际样品中进行的峰回收率实验验证了该方法的可靠性和可行性，回收率为95.34% ~ 104.28%，相对标准偏差为0.29% ~ 5.06%，均在可接受的标准范围内。本研究不仅为高效合成纳米酶提供了可靠的策略，也为AA检测提供了一种方便的方法，帮助个体做出合理健康的饮食选择。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.