An all-in-one assay for bacteria-related detections through gold nanoparticles-amplified electrical conductivity

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-09-15 DOI:10.1007/s00604-025-07536-9

Shicheng Su, Jing Hu, Yichun Zhu, Bing Liang, Yaping Zhu, Shuifeng Zhang, Huadong Sheng, Hongbo Chen, Yunlei Xianyu, Chunyan He

{"title":"An all-in-one assay for bacteria-related detections through gold nanoparticles-amplified electrical conductivity","authors":"Shicheng Su, Jing Hu, Yichun Zhu, Bing Liang, Yaping Zhu, Shuifeng Zhang, Huadong Sheng, Hongbo Chen, Yunlei Xianyu, Chunyan He","doi":"10.1007/s00604-025-07536-9","DOIUrl":null,"url":null,"abstract":"<div><p> An all-in-one assay based on the electrical conductivity (EC) has been developed that enables the viability test and the specific detection of pathogenic bacteria, including the differentiation of (1) live/dead bacteria, (2) drug-sensitive/resistant bacteria, (3) Gram-positive/negative bacteria, and (4) specific detection of <i>A. baumannii</i>. Key to this assay is the modification of gold nanoparticles on the surface of bacteria that can amplify the EC signal for enhanced sensitivity and rapid detection. The amplification effect of gold nanoparticles significantly reduces the time and enhances the sensitivity for bacteria detection. This EC-based assay outcompetes traditional colorimetric and fluorescent methods owing to its rapid response, convenient operation, and low cost, which holds great promise for biosensing.</p><h3>Graphical abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07536-9","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

An all-in-one assay based on the electrical conductivity (EC) has been developed that enables the viability test and the specific detection of pathogenic bacteria, including the differentiation of (1) live/dead bacteria, (2) drug-sensitive/resistant bacteria, (3) Gram-positive/negative bacteria, and (4) specific detection of A. baumannii. Key to this assay is the modification of gold nanoparticles on the surface of bacteria that can amplify the EC signal for enhanced sensitivity and rapid detection. The amplification effect of gold nanoparticles significantly reduces the time and enhances the sensitivity for bacteria detection. This EC-based assay outcompetes traditional colorimetric and fluorescent methods owing to its rapid response, convenient operation, and low cost, which holds great promise for biosensing.

Graphical abstract

查看原文本刊更多论文

通过金纳米粒子放大电导率进行细菌相关检测的一体化检测

已经开发出一种基于电导率（EC）的一体化检测方法，可以进行活力测试和致病菌的特异性检测，包括(1)活菌/死菌，(2)药敏/耐药菌，(3)革兰氏阳性/阴性菌的区分，以及(4)鲍曼不动杆菌的特异性检测。该试验的关键是对细菌表面的金纳米颗粒进行修饰，可以放大EC信号，提高灵敏度和快速检测。金纳米粒子的扩增效应显著缩短了细菌检测时间，提高了检测灵敏度。该方法反应迅速，操作方便，成本低，在生物传感领域具有广阔的应用前景，因此优于传统的比色法和荧光法。图形抽象

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.