A multi-thionine DNA network-driven electrochemical aptasensor with dual signal amplification for ultrasensitive interleukin-6 detection

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-09-12 DOI:10.1007/s00604-025-07322-7

Linru Bai, Benqi Chen, Yan Xu, Mingjia Liu, Yunhui Yang, Jianmei Yang, Rong Hu

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引用次数: 0

Abstract

Au NPs@FGO@Fe MOG were sythesized for its inaugural application in biosensor development and engineered as a novel, highly efficient electrochemical aptasensor for the precise detection of interleukin-6 (IL-6). The aptasensor architecture integrates Au NPs@FGO@Fe MOG as the electrode interface and an anemone-like PtNi nanoclusters@MIL-anchored three-dimensional DNA (PD) network as the signal transduction matrix. The as-prepared Au NPs@FGO@Fe MOG exhibited an exceptionally high specific surface area, excellent electrical conductivity, and robust structural stability, collectively contributing to a pronounced enhancement in the performance of an electrochemical biosensor. The hierarchically PD network provided abundant binding sites for the redox-active probe thionine (Thi), thereby amplifying the electrochemical signal with remarkable efficiency. Under optimized conditions, the aptasensor achieved an impressive detection limit of 0.89 pg/mL. Moreover, the platform demonstrated reliable performance in detecting IL-6 in unspiked human serum samples, with results exhibiting high concordance with those obtained from commercial enzyme-linked immunosorbent assay (ELISA) kits. These findings demonstrate the considerable potential of this platform for rapid, sensitive, and accurate cytokine quantification, offering valuable prospects for clinical diagnostic applications.

Graphical Abstract

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一种多硫氨酸DNA网络驱动的双信号放大电化学配体传感器，用于超灵敏的白细胞介素-6检测

Au NPs@FGO@Fe MOG是为其在生物传感器开发中的首次应用而合成的，并被设计为一种新型的、高效的电化学适配传感器，用于精确检测白细胞介素-6 （IL-6）。aptassensor架构将Au NPs@FGO@Fe MOG作为电极界面，将类似海葵的PtNi nanoclusters@MIL-anchored三维DNA （PD）网络作为信号转导矩阵。制备的Au NPs@FGO@Fe MOG具有极高的比表面积、优异的导电性和强大的结构稳定性，这些都显著提高了电化学生物传感器的性能。分层PD网络为氧化还原活性探针硫氨酸（Thi）提供了丰富的结合位点，从而以显著的效率放大电化学信号。在优化条件下，该传感器的检出限为0.89 pg/mL。此外，该平台在检测未加标人血清样本中的IL-6方面表现出可靠的性能，其结果与商用酶联免疫吸附测定（ELISA）试剂盒的结果高度一致。这些发现证明了该平台在快速、敏感和准确的细胞因子定量方面的巨大潜力，为临床诊断应用提供了宝贵的前景。图形抽象

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.