{"title":"基于V2CTx前体的v - pmof衍生多孔纳米材料制备氢化可的松电化学感应传感器","authors":"Zhuo Shi, Shuyi Yang, Zhenxia Ma, Zifeng Wang, Zhanhong Li, Linlin Wang, Ľubomír Švorc, Zhigang Zhu","doi":"10.1007/s00604-025-07404-6","DOIUrl":null,"url":null,"abstract":"<p>An electrochemical aptasensor for hydrocortisone (HC) detection was developed using a vanadium-based porphyrinic metal–organic framework (V-PMOF) materials synthesized from vanadium-based MXene (V₂CTₓ) and tetrakis (4-carboxyphenyl) porphyrin (TCPP), followed by thermal treatment at 800 °C to obtain V-PMOF<sub>800°C</sub>. The resulting V-PMOF<sub>800°C</sub> retained a nanoflower-like porous structure, enhancing electron transport, structural integrity, and electroactive site accessibility. Gold nanoparticles (Au NPs) were electrodeposited onto the V-PMOF<sub>800°C</sub>-modified electrode to improve conductivity and enable thiolated DNA aptamer (DNA<sub>apt</sub>) immobilization via Au–S bonding. Electrochemical measurements, particularly differential pulse voltammetry (DPV), were employed to evaluate the performance of the aptasensor, which exhibited a wide linear concentration range (0.05 nM–5 μM) and a low detection limit (LOD) of 10 pM. This work introduces a promising strategy for constructing MXene or MOF-based aptasensors with high sensitivity, selectivity, and stability for practical hormone monitoring applications.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"An electrochemical aptasensor for hydrocortisone detection using V-PMOF-derived porous nanomaterials from V2CTx precursors\",\"authors\":\"Zhuo Shi, Shuyi Yang, Zhenxia Ma, Zifeng Wang, Zhanhong Li, Linlin Wang, Ľubomír Švorc, Zhigang Zhu\",\"doi\":\"10.1007/s00604-025-07404-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>An electrochemical aptasensor for hydrocortisone (HC) detection was developed using a vanadium-based porphyrinic metal–organic framework (V-PMOF) materials synthesized from vanadium-based MXene (V₂CTₓ) and tetrakis (4-carboxyphenyl) porphyrin (TCPP), followed by thermal treatment at 800 °C to obtain V-PMOF<sub>800°C</sub>. The resulting V-PMOF<sub>800°C</sub> retained a nanoflower-like porous structure, enhancing electron transport, structural integrity, and electroactive site accessibility. Gold nanoparticles (Au NPs) were electrodeposited onto the V-PMOF<sub>800°C</sub>-modified electrode to improve conductivity and enable thiolated DNA aptamer (DNA<sub>apt</sub>) immobilization via Au–S bonding. Electrochemical measurements, particularly differential pulse voltammetry (DPV), were employed to evaluate the performance of the aptasensor, which exhibited a wide linear concentration range (0.05 nM–5 μM) and a low detection limit (LOD) of 10 pM. This work introduces a promising strategy for constructing MXene or MOF-based aptasensors with high sensitivity, selectivity, and stability for practical hormone monitoring applications.</p>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 10\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-09-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-025-07404-6\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07404-6","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
An electrochemical aptasensor for hydrocortisone detection using V-PMOF-derived porous nanomaterials from V2CTx precursors
An electrochemical aptasensor for hydrocortisone (HC) detection was developed using a vanadium-based porphyrinic metal–organic framework (V-PMOF) materials synthesized from vanadium-based MXene (V₂CTₓ) and tetrakis (4-carboxyphenyl) porphyrin (TCPP), followed by thermal treatment at 800 °C to obtain V-PMOF800°C. The resulting V-PMOF800°C retained a nanoflower-like porous structure, enhancing electron transport, structural integrity, and electroactive site accessibility. Gold nanoparticles (Au NPs) were electrodeposited onto the V-PMOF800°C-modified electrode to improve conductivity and enable thiolated DNA aptamer (DNAapt) immobilization via Au–S bonding. Electrochemical measurements, particularly differential pulse voltammetry (DPV), were employed to evaluate the performance of the aptasensor, which exhibited a wide linear concentration range (0.05 nM–5 μM) and a low detection limit (LOD) of 10 pM. This work introduces a promising strategy for constructing MXene or MOF-based aptasensors with high sensitivity, selectivity, and stability for practical hormone monitoring applications.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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