Canan Armutcu, Sena Pişkin, Erdoğan Özgür, Mustafa Karakaya, M. Emin Çorman, Lokman Uzun
{"title":"用于次黄嘌呤检测的纳米碳纤维增强分子印迹电化学传感器","authors":"Canan Armutcu, Sena Pişkin, Erdoğan Özgür, Mustafa Karakaya, M. Emin Çorman, Lokman Uzun","doi":"10.1007/s00604-025-07502-5","DOIUrl":null,"url":null,"abstract":"<div><p>A molecularly imprinted electrochemical sensor (MIP) was developed using thymine-functionalized carbon nanofibers (Thy@CNFs) to enable selective detection of hypoxanthine (HYP). The sensor was fabricated by first depositing Thy@CNFs onto a glassy carbon electrode (GCE), followed by electropolymerization of a pyrrole-co-pyrrole-3-carboxylic acid (Py-co-PyCOOH) copolymer in the presence of HYP. Each modification step was characterized using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), and contact angle measurements. Under optimized conditions, the Thy@CNFs-modified MIP sensor (Thy@CNFs/MIP/GCE) exhibited a linear response to HYP concentrations ranging from 1 × 10<sup>−9</sup> to 1 × 10<sup>−8</sup> M, with a detection limit of 1.71 × 10<sup>−10</sup> M. Finally, the sensor was successfully applied to commercial serum and artificial urine sample, achieving recoveries of 99.55% and 100.17%, respectively, demonstrating its accuracy, precision, and practical applicability in real sample analysis.\n</p><h3>Graphical Abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 10","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Carbon nanofiber–enhanced molecular imprinted electrochemical sensor for hypoxanthine detection\",\"authors\":\"Canan Armutcu, Sena Pişkin, Erdoğan Özgür, Mustafa Karakaya, M. Emin Çorman, Lokman Uzun\",\"doi\":\"10.1007/s00604-025-07502-5\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>A molecularly imprinted electrochemical sensor (MIP) was developed using thymine-functionalized carbon nanofibers (Thy@CNFs) to enable selective detection of hypoxanthine (HYP). The sensor was fabricated by first depositing Thy@CNFs onto a glassy carbon electrode (GCE), followed by electropolymerization of a pyrrole-co-pyrrole-3-carboxylic acid (Py-co-PyCOOH) copolymer in the presence of HYP. Each modification step was characterized using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), and contact angle measurements. Under optimized conditions, the Thy@CNFs-modified MIP sensor (Thy@CNFs/MIP/GCE) exhibited a linear response to HYP concentrations ranging from 1 × 10<sup>−9</sup> to 1 × 10<sup>−8</sup> M, with a detection limit of 1.71 × 10<sup>−10</sup> M. Finally, the sensor was successfully applied to commercial serum and artificial urine sample, achieving recoveries of 99.55% and 100.17%, respectively, demonstrating its accuracy, precision, and practical applicability in real sample analysis.\\n</p><h3>Graphical Abstract</h3>\\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 10\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2025-09-12\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-025-07502-5\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-025-07502-5","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Carbon nanofiber–enhanced molecular imprinted electrochemical sensor for hypoxanthine detection
A molecularly imprinted electrochemical sensor (MIP) was developed using thymine-functionalized carbon nanofibers (Thy@CNFs) to enable selective detection of hypoxanthine (HYP). The sensor was fabricated by first depositing Thy@CNFs onto a glassy carbon electrode (GCE), followed by electropolymerization of a pyrrole-co-pyrrole-3-carboxylic acid (Py-co-PyCOOH) copolymer in the presence of HYP. Each modification step was characterized using electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV), attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electron microscopy (SEM), and contact angle measurements. Under optimized conditions, the Thy@CNFs-modified MIP sensor (Thy@CNFs/MIP/GCE) exhibited a linear response to HYP concentrations ranging from 1 × 10−9 to 1 × 10−8 M, with a detection limit of 1.71 × 10−10 M. Finally, the sensor was successfully applied to commercial serum and artificial urine sample, achieving recoveries of 99.55% and 100.17%, respectively, demonstrating its accuracy, precision, and practical applicability in real sample analysis.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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