Asian Pacific Journal of Cancer Prevention最新文献

{"title":"Mixed Methods Study of Care Pathway and Delay in Obtaining Health Services by Breast Cancer Patients in India.","authors":"Radhika Kuthari, Raman Damor, Kalpita Shringarpure","doi":"10.31557/APJCP.2025.26.6.2097","DOIUrl":"10.31557/APJCP.2025.26.6.2097","url":null,"abstract":"<p><p>Delay in receiving treatment or untimely discontinuation can impact patient survival. This study addresses the literature gap in knowledge about care pathway of patients following initiation of treatment, quantifies the delays in obtaining healthcare services and explores the challenges faced by them in this process.</p><p><strong>Methods: </strong>A concurrent triangulation mixed method study was conducted. All breast cancer patients above 18 years, who were aware of their diagnosis and whose treatment had commenced were included with their consent. The final sample size was 150. Patient pathway was documented through in-depth interviews and delay in access, diagnosis, and various treatment modalities was quantified after reviewing patient records. Factors associated with overall delay (from onset of symptoms to initiation of treatment) were identified by bivariate analysis. Inductive thematic analysis of interviews was done to identify reasons for delay.</p><p><strong>Results: </strong>Of 150 patients, 111 (74%) consulted at least two healthcare facilities and 75 (50%) consulted only government facilities during their treatment.  Majority, 124 (82.6%) patients faced delay in their care pathway; 88 (58.6%) experienced overall delay (median duration 109 days) which was significantly associated with patient's education and caregiver's occupation (p<0.05), and 48 (32%) out of 149 women were delayed in initiation of chemotherapy. Median access, diagnostic and treatment interval were 60 days, 20 days, and 6 days respectively. Median duration between surgery and chemotherapy, and surgery and radiotherapy was 10 days and 139 days respectively. The major themes that emerged in access delay were neglect, avoidance, and reliance on family. Diagnostic delay included themes of misdiagnosis and difficulty in navigating healthcare systems. Treatment delay included themes of misconceptions, avoidance, neglect, and affordability and accessibility of services.</p><p><strong>Conclusion: </strong>Majority of the patients visited at least two facilities and experienced delay in receiving healthcare services. Both patient and provider side challenges were responsible for delay.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"2097-2107"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protecting HPV Vaccination Acceptance Among Parents 5th and 6th Grade Elementary School in Indonesia.","authors":"Nurul Mardiati, Deni Setiawan, Samsul Hadi, Nashrul Wathan","doi":"10.31557/APJCP.2025.26.6.2043","DOIUrl":"10.31557/APJCP.2025.26.6.2043","url":null,"abstract":"<p><strong>Objective: </strong>This study seeks to determine parents' intentions to accept HPV vaccination for their daughters in the 5th and 6th grades elementary school in Indonesia to support future HPV vaccine acceptance.</p><p><strong>Methods: </strong>Eligible participants from all provinces in Indonesia were recruited using a 25-item questionnaire. We summarized the sociodemographic data and mortgage scores of the participants using descriptive statistics. To identify independent determinants of vaccination intentions, we conducted bivariate and multivariate ordinal logistic regression analysis.</p><p><strong>Result: </strong>A total of 1,000 parents responded. Overall, only 48.7% intended to receive HPV vaccination. In addition, 56.3% exhibited negative attitudes, 50.3% reported negative subjective norms, and 60.5% demonstrated negative perceived behavioral control. Subjective norms were significantly associated with HPV vaccination acceptance intentions (p-value = 0.000; Exp(B) 7.020).</p><p><strong>Conclusion: </strong>Participants demonstrated moderately high intentions to refuse HPV vaccination, with subjective norms being a significant influencing factor. Therefore, to increase vaccine acceptance, targeted interventions aimed to improving subjective norms are essential.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"2043-2048"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methylation of Tumor Suppressor Genes on Chromosome 9: Diagnostic Insights from a Nasopharyngeal Carcinoma in Vietnam.","authors":"Hue Hong Thieu, Thuy Ai Huyen Le, Thuan Duc Lao","doi":"10.31557/APJCP.2025.26.6.2239","DOIUrl":"10.31557/APJCP.2025.26.6.2239","url":null,"abstract":"<p><strong>Background: </strong>Methylation represents the second hit in tumor development. In nasopharyngeal carcinoma (NPC), multiple tumor suppressor genes located on chromosome 9 under methylation.</p><p><strong>Aims: </strong>This first case-control study aims to explore the methylation characteristics of DAPK, p16INK4α, p15INK4α, and p14ARF, individually and in combination, to evaluate their potential as promising biomarkers for nasopharyngeal carcinoma (NPC).</p><p><strong>Materials and methods: </strong>A total of 70 NPC biopsy samples and 60 non-cancerous swab samples were enrolled into the current study. Genomic DNA extraction, bisulfite modification, and methylation-specific PCR (MSP) were used to analyze methylation status. Statistical analyses, including odds ratio (OR) calculations and methylation index (MI) evaluation, were conducted to determine the association between methylation and nasopharyngeal carcinoma (NPC).</p><p><strong>Results: </strong>Promoter hypermethylation was observed in 75.71%, 78.57%, 52.86%, and 87.14% of samples for DAPK, p16INK4α, p15INK4α, and p14ARF, respectively. A significant association between the methylation status of these candidate genes and nasopharyngeal carcinoma (NPC) was identified. The methylation of these genes significantly increased the risk of NPC compared to control samples (OR > 1). Based on the methylation index (MI), 100% of NPC samples showed methylation in at least one gene (MI ≥ 0.25). An MI ≥ 0.25 demonstrated a sensitivity of 100% and a specificity of 50%, highlighting its potential as a diagnostic marker.</p><p><strong>Conclusion: </strong>This study highlights the potential of DAPK, p16INK4α, p15INK4α, and p14ARF methylation as biomarkers for the diagnosis and early screening of nasopharyngeal carcinoma (NPC) in Vietnamese patients. The findings support the use of a composite methylation index as a sensitive biomarker for the early detection and screening of NPC.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"2239-2245"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Anticancer Effect of Haematococcus Pluvialis Alga Extract on Breast Cancer Cell Line.","authors":"Vahideh Shamskhani, Habib Zarredar, Jafar Razeghi, Atefeh Ansarin, Milad Asadi, Zahra Soleimani, Ensiyeh Seyedrezazadeh","doi":"10.31557/APJCP.2025.26.6.2071","DOIUrl":"10.31557/APJCP.2025.26.6.2071","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to investigate the anticancer effects of H. pluvialis extract on breast cancer.  Methods: We evaluated the cytotoxicity, suppression of migration, and apoptosis ability of H. pluvialis alga extract MDA-MB-468 by MTT assay, scratch test, and flow cytometry, respectively. The expression patterns of MMP1 and MMP9, Bax and Bcl2, Caspase3, Caspase6, and Caspase9 were analyzed by qRT-PCR.</p><p><strong>Results: </strong>Based on the findings of this study, the survival and proliferation capacity of MDA-MB-468 cells treated with the extract was reduced. Flow cytometry results showed that treatment with H. Pluvialis extracts induced apoptosis. Scratch test results showed that H. Pluvialis extract could inhibit cell migration. The expression patterns of the studied genes were changed by treatment with the extract. Expression of Bcl2, MMP1, and MMP9 genes decreased, and expression of Bax, Caspase3, Caspase8, Caspase9 genes increased.</p><p><strong>Conclusion: </strong>The findings of this research reveal that H. pluvialis algae extract shows a potential anti-cancer compound.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 6","pages":"2071-2077"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Malnutrition in Colorectal Cancer Patients: Association with the Lack of Eating Motivation and Inappropriate Diet.","authors":"Le Thi Ngoc Anh, To Gia Kien, Nguyen Van Tuan, Tran Thi Anh Tuong, Juyeon Ko, Phan Tan Dan, Jaelim Cho, Nguyen Van Tap","doi":"10.31557/APJCP.2025.26.5.1661","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.5.1661","url":null,"abstract":"<p><strong>Objective: </strong>Colorectal cancer (CRC) significantly contributes to cancer-related mortality in Vietnam. Notably, malnutrition, rather than cancer itself, accounts for one-fifth of the deaths among cancer patients. Therefore, understanding the nutritional status and related factors among CRC patients is essential. We aimed to investigate the nutritional status and related factors in CRC in Vietnam.</p><p><strong>Methods: </strong>This cross-sectional study, conducted from October 2022 to April 2023, included CRCs of both sexes aged ≥18 years. Data collection involved face-to-face interviews, anthropometric assessments, and medical record reviews. Nutritional status was evaluated using the Patient-Generated Subjective Global Assessment (PG-SGA). Multivariable logistic regression was used to identify malnutrition-related factors.</p><p><strong>Results: </strong>In total, 388 patients were included (median age, 60.0 years, [IQR: 51.0-66.0 years]; men, 57.7%). The prevalence of malnutrition was 87.9% (95%CI: 84.6-91.1), and urgent nutritional intervention was needed in 64.7% of participants. Malnutrition-associated factors included lack of eating motivation (OR=8.76, 95%CI: 1.81-42.38), dieting for fear of cancer cell growth (OR=3.82, 95%CI: 1.27-11.52), gastrointestinal symptoms (OR=5.38, 95%CI: 1.76-16.45), daily energy intake <25kcal/kg (OR=7.02, 95%CI: 1.70-28.99), protein ≤ 1g/kg (OR=5.21, 95%CI: 1.32-20.60), fat <18% of total energy intake (OR=3.13, 95%CI: 1.02-9.57), mean corpuscular volume <85fL (OR=4.74, 95%CI: 1.11-20.22), and total lymphocyte count ≤1700 lymphocytes/mm3 (OR=4.06, 95%CI: 1.22-13.50). Additionally, a 1-kg increase in dominant hand strength reduced the risk of malnutrition by 4% (OR=0.96, 95%CI: 0.93-0.99).</p><p><strong>Conclusion: </strong>The high prevalence of malnutrition among CRCs in Vietnam necessitates nutritional intervention. The main contributors include loss of eating motivation and inadequate dietary intake.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 5","pages":"1661-1670"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medicago sativa Extracts Enhance the Anticancer Efficacy of GEM in PANC-1 Cells through Apoptosis Induction and BAX/BCL-2/CASP3 Expression Modulation.","authors":"Nazanin Jamshidi, Negar Jamshidi, Mohammad Zaman, Mahta Chehrehsaz, Farnaz Roshanfarzad, Vahid Chaleshi, Hamid Asadzadeh Aghdaei","doi":"10.31557/APJCP.2025.26.5.1689","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.5.1689","url":null,"abstract":"<p><strong>Introduction: </strong>Pancreatic cancer (PC) has a poor prognosis and limited response to therapies. Combinatorial approaches, such as natural product-based therapies, can enhance anticancer efficacy while minimizing side effects. This study evaluated M. sativa's anticancer properties and its potential as adjunctive therapy with Gemcitabine (GEM) to sensitize PANC-1 cells to chemotherapy.</p><p><strong>Methods: </strong>The antioxidant activity (AA) and total phenolic content (TPC) of M. sativa extracts (Methanol, Ethyl acetate, and water) were assessed using the DPPH radical scavenging assay. Cytotoxic effects on PANC1 and HUVEC cells were also evaluated by utilizing the MTT assay. Then, apoptosis detection was performed by Annexin V/PI-flow cytometry (FC). Besides, the DNA fragmentation analysis was conducted utilizing agarose gel electrophoresis (AGE). Bcl-2, Bax, and CASP3 expression levels in PANC-1 cells using western blot analysis and qRT-PCR.</p><p><strong>Results: </strong>Herein, DPPH IC50 values for M. sativa extracts (water, MeOH, EtOH) were 76.21, 110.32, and 65.39 μg/ml, respectively. The water extract of M. sativa exhibited the highest TPC (4612.15 ± 119.4 mgGAE/g). The cytotoxicity IC50 values for EtOH M. sativa extract, GEM, and combined GEM with EtOH M. sativa on PANC1 cells were 68.74, 43.53, and 41.22 µg/ml M. sativa + 25 µg/ml GEM, respectively, with no toxicity observed in HUVEC cells. FC analysis revealed that Combining GEM and EtOH M. sativa yielded the highest apoptosis rate (25.6%). Expression changes in Bcl-2, Bax, and CASP3, as well as morphological alterations and DNA fragmentation, indicated apoptotic cell death.</p><p><strong>Conclusion: </strong>Our findings suggested that combining M.sativa EtOH extracts with GEM may represent a promising strategy for treating PC.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 5","pages":"1689-1700"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of In Vitro Anticancer Activity and Apoptotic Potential of Wrightia Tinctoria Bark Extracts on Oral Cancer Cell Lines.","authors":"Deepa Anchalayil Gopalakrishnan, Beena Valappil Thannikunnath, Rajesh Ramachandran, Sudha Sivasankar","doi":"10.31557/APJCP.2025.26.5.1753","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.5.1753","url":null,"abstract":"<p><strong>Background and objectives: </strong>Bioprospecting of indigenous plants for biological/cytotoxic properties has become an area of growing interest in cancer research. Wrightia tinctoria is a lesser-studied plant widely distributed in India which is commonly used in traditional medicinal systems like Ayurveda and Siddha and holds potential as a source for novel therapeutic agents. In this study, the cytotoxic effects of Wrightia tinctoria bark extracts on oral cancer (KB) cell lines were evaluated, focusing on three different extraction solvents ethanol, ethyl acetate, and water. The present study aimed to evaluate and compare the cytotoxic effects of Wrightia tinctoria bark extracts, while also investigating their potential to induce apoptosis which can contribute into exploration of new plant based anticancer agents.</p><p><strong>Methods: </strong>Cytotoxic effects of ethanol, ethyl acetate, and aqueous extracts of Wrightia tinctoria bark were evaluated on human oral cancer cells (KB cell line). Cell viability was assessed by 3-(4,5-dimethylthiazoline- 2-yl)-2,5-diphenyltetrazolium bromide (MTT assay). Apoptosis was evaluated by acridine orange (AO) and ethidium bromide (EB) double staining method and expression of apoptotic enzymes Caspase 3 and Caspase 7 were measured by quantitative indirect ELISA technique.</p><p><strong>Results: </strong>Ethanol extract of Wrightia tinctoria bark exhibited highest cytotoxic activity towards oral cancer cell lines. The half-maximum inhibitory concentration (IC50) values of the ethanol, ethyl acetate, aqueous extracts were 75.084µg/ml, 179.743µg/ml and 115.258 µg/ml respectively.  IC 50 concentration of ethanol and ethyl acetate extracts stimulated significant apoptosis in KB cells. Indirect ELISA showed increased expression of caspase 3 and caspase 7 among the cells on treatment with ethanol  and ethyl acetate extracts.</p><p><strong>Conclusion: </strong>Ethanol extract of Wrightia tinctoria bark exhibited highest cytotoxic activity towards oral cancer cell lines and induced apoptotic changes. Further research may help us to identify newer, better anti cancer agents against oral cancer from Wrightia tinctoria bark.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 5","pages":"1753-1759"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Tobacco Consumption on Salivary Protein Profiles: A MALDI-TOF Mass Spectrometry Study.","authors":"Manish S, Ramya Ramadoss, Nitya Krishnasamy, Sandhya S, Suganya Panneer Selvam, Hemashree K","doi":"10.31557/APJCP.2025.26.5.1483","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.5.1483","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the impact of tobacco consumption on salivary protein profiles and identify proteomic alterations associated with tobacco use, using advanced mass spectrometry techniques.</p><p><strong>Methods: </strong>This cross-sectional study involved 100 adults aged 18-60, divided into two groups: 50 tobacco users (smokers and smokeless users) and 50 non-users. Participants were selected based on strict inclusion criteria, excluding individuals with systemic diseases, ongoing medications, or cancer history to minimize confounding factors. Unstimulated saliva samples were collected under standardized conditions, centrifuged, and stored at -80°C. Proteins were extracted using trichloroacetic acid (TCA) precipitation, quantified via Bradford assay, and analyzed with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS). Bioinformatics tools, including Cytoscape and heatmap analysis, were utilized to identify protein interaction networks and significant proteomic alterations. Statistical significance was set at p < 0.05.</p><p><strong>Results: </strong>Tobacco users exhibited significant proteomic alterations compared to non-users, with marked upregulation of inflammatory proteins such as TRML1, MAFB (p < 0.05), and NINJ1, indicating chronic inflammation. Oxidative stress markers, including mitochondrial proteins NU4M and GSAS1, were significantly elevated (p < 0.01), reflecting tobacco-induced cellular stress. DNA repair proteins like O6C70 and PP4RL showed increased expression (p < 0.05), suggesting an adaptive response to smoking-induced DNA damage. Cancer-related proteins TWST1 and CD82 were upregulated (p < 0.05), highlighting processes linked to carcinogenesis, such as epithelial-mesenchymal transition (EMT). Cytoscape analysis revealed highly interconnected protein networks in smokers, with central nodes involving JUNB, EGR1, and FOSB, indicating systemic biological disruptions. In contrast, non-smokers displayed modular and compartmentalized networks reflecting stable cellular processes. Heatmap analysis further emphasized significant differences in protein expression patterns, with smokers showing elevated levels of stress-related proteins while non-smokers exhibited a balanced proteomic profile associated with immune homeostasis and metabolic regulation.</p><p><strong>Conclusion: </strong>Tobacco consumption significantly alters salivary protein profiles, promoting chronic inflammation, oxidative stress, and DNA repair dysregulation, all of which may contribute to oral carcinogenesis. These findings emphasize the utility of MALDI-TOF MS in detecting salivary biomarkers for early diagnosis of tobacco-related oral diseases and provide insights into the molecular pathways disrupted by tobacco use.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 5","pages":"1483-1489"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Silico Analysis Revealed a Role for NUSAP1 in Lung Adenocarcinoma through E2F1/hsa-let-7b-5p/lncRNA-TMPO-AS1.","authors":"Sakshi Nirmal, Prerna Vats, Rajeev Nema","doi":"10.31557/APJCP.2025.26.5.1793","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.5.1793","url":null,"abstract":"<p><strong>Objectives: </strong>Nucleolar and spindle-associated protein 1 (NUSAP1) is crucial for chromosomal segregation and spindle assembly, its expression correlates with high morbidity and mortality rates, necessitating better understanding of prognosis-related networks.</p><p><strong>Methods: </strong>The study used databases like KM Plotter, TNMplot, UALCAN, OncoMX, GEPIA2, OncoDB, ENCORI, TIMER 2.0, CancerSEA, miRNet, CellTracer, TISIDB, GSCA, and the Enrichr database to analyze NUSAP1 expression in lung cancer tumors and normal tissues.</p><p><strong>Results: </strong>The NUSAP1 gene is overexpressed in both adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC). However, LUAD has a poor prognosis for overall survival (OS) (HR = 1.94), first progression (FP) (HR = 1.96), and post-progression survival (PPS) (HR = 1.48), while LUSC showed no significant data. High NUSAP1 expression is significantly associated with adenocarcinoma smoker patients. The study also found a strong correlation between lncRNA-TMPO-AS1 overexpression and poor OS prognosis in LUAD smokers, a negative relationship between miRNA hsa-let-7b-5p and TMPO-AS1/E2F1/NUSAP1 expression, and a positive correlation between S and M phase cell cycle checkpoints, tumor infiltrating CD4 immune cells, and NUSAP1 expression in lung adenocarcinoma.</p><p><strong>Conclusion: </strong>Smokers with lung adenocarcinoma have worse prognoses due to higher E2F1, NUSAP1, and TMPO-AS1 levels, possibly due to TMPO-AS1 sponge formation with hsa-let-7b-5p.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 5","pages":"1793-1807"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144175898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction.","authors":"","doi":"10.31557/APJCP.2025.26.5.1863","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.5.1863","url":null,"abstract":"","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 5","pages":"1863"},"PeriodicalIF":0.0,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}