通过E2F1/hsa-let-7b-5p/lncRNA-TMPO-AS1在肺腺癌中的作用

Q2 Medicine

Asian Pacific Journal of Cancer Prevention Pub Date : 2025-05-01 DOI:10.31557/APJCP.2025.26.5.1793

Sakshi Nirmal, Prerna Vats, Rajeev Nema

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引用次数: 0

摘要

目的：核仁和纺锤体相关蛋白1 （NUSAP1）对染色体分离和纺锤体组装至关重要，其表达与高发病率和死亡率相关，需要更好地了解预后相关网络。方法：采用KM Plotter、TNMplot、UALCAN、OncoMX、GEPIA2、OncoDB、ENCORI、TIMER 2.0、CancerSEA、miRNet、CellTracer、TISIDB、GSCA、富集等数据库分析NUSAP1在肺癌肿瘤和正常组织中的表达。结果：NUSAP1基因在腺癌（LUAD）和鳞状细胞癌（LUSC）中均有过表达。然而，LUAD在总生存期（OS）（HR = 1.94）、首次进展期（FP）（HR = 1.96）和进展后生存期（PPS）（HR = 1.48）方面预后较差，而LUSC无显著数据。NUSAP1高表达与腺癌吸烟者显著相关。本研究还发现，LUAD吸烟者lncRNA-TMPO-AS1过表达与OS预后不良密切相关，miRNA hsa-let-7b-5p与TMPO-AS1/E2F1/NUSAP1表达呈负相关，肺腺癌中S期和M期细胞周期检查点、肿瘤浸润CD4免疫细胞、NUSAP1表达呈正相关。结论：吸烟者肺腺癌患者由于较高的E2F1、NUSAP1和TMPO-AS1水平，可能是由于TMPO-AS1海绵与hsa-let-7b-5p形成所致，预后较差。

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In Silico Analysis Revealed a Role for NUSAP1 in Lung Adenocarcinoma through E2F1/hsa-let-7b-5p/lncRNA-TMPO-AS1.

Objectives: Nucleolar and spindle-associated protein 1 (NUSAP1) is crucial for chromosomal segregation and spindle assembly, its expression correlates with high morbidity and mortality rates, necessitating better understanding of prognosis-related networks.

Methods: The study used databases like KM Plotter, TNMplot, UALCAN, OncoMX, GEPIA2, OncoDB, ENCORI, TIMER 2.0, CancerSEA, miRNet, CellTracer, TISIDB, GSCA, and the Enrichr database to analyze NUSAP1 expression in lung cancer tumors and normal tissues.

Results: The NUSAP1 gene is overexpressed in both adenocarcinoma (LUAD) and squamous cell carcinoma (LUSC). However, LUAD has a poor prognosis for overall survival (OS) (HR = 1.94), first progression (FP) (HR = 1.96), and post-progression survival (PPS) (HR = 1.48), while LUSC showed no significant data. High NUSAP1 expression is significantly associated with adenocarcinoma smoker patients. The study also found a strong correlation between lncRNA-TMPO-AS1 overexpression and poor OS prognosis in LUAD smokers, a negative relationship between miRNA hsa-let-7b-5p and TMPO-AS1/E2F1/NUSAP1 expression, and a positive correlation between S and M phase cell cycle checkpoints, tumor infiltrating CD4 immune cells, and NUSAP1 expression in lung adenocarcinoma.

Conclusion: Smokers with lung adenocarcinoma have worse prognoses due to higher E2F1, NUSAP1, and TMPO-AS1 levels, possibly due to TMPO-AS1 sponge formation with hsa-let-7b-5p.

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来源期刊

Asian Pacific Journal of Cancer Prevention 医学-肿瘤学

CiteScore

2.80

自引率

0.00%

发文量

779

审稿时长

3 months

期刊介绍： Cancer is a very complex disease. While many aspects of carcinoge-nesis and oncogenesis are known, cancer control and prevention at the community level is however still in its infancy. Much more work needs to be done and many more steps need to be taken before effective strategies are developed. The multidisciplinary approaches and efforts to understand and control cancer in an effective and efficient manner, require highly trained scientists in all branches of the cancer sciences, from cellular and molecular aspects to patient care and palliation. The Asia Pacific Organization for Cancer Prevention (APOCP) and its official publication, the Asia Pacific Journal of Cancer Prevention (APJCP), have served the community of cancer scientists very well and intends to continue to serve in this capacity to the best of its abilities. One of the objectives of the APOCP is to provide all relevant and current scientific information on the whole spectrum of cancer sciences. They aim to do this by providing a forum for communication and propagation of original and innovative research findings that have relevance to understanding the etiology, progression, treatment, and survival of patients, through their journal. The APJCP with its distinguished, diverse, and Asia-wide team of editors, reviewers, and readers, ensure the highest standards of research communication within the cancer sciences community across Asia as well as globally. The APJCP publishes original research results under the following categories: -Epidemiology, detection and screening. -Cellular research and bio-markers. -Identification of bio-targets and agents with novel mechanisms of action. -Optimal clinical use of existing anti-cancer agents, including combination therapies. -Radiation and surgery. -Palliative care. -Patient adherence, quality of life, satisfaction. -Health economic evaluations.