Asian Pacific Journal of Cancer Prevention最新文献

{"title":"HPV Vaccination Program in Indonesia: Effectiveness, Dose, Scale-Up Costs, Future Prospects, and Policy Recommendations.","authors":"Didik Setiawan, Putri Ramadani, Lianawati Lianawati, Githa Fungie Galistiani","doi":"10.31557/APJCP.2025.26.2.421","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.2.421","url":null,"abstract":"<p><strong>Background: </strong>Among females, Cervical cancer affects more frequently than any other type of cancer in Indonesia. Cervical cancer and illnesses linked to HPV infection are potentially preventable through vaccination.  The aim of his study was to describe the characteristics of the available vaccines, the  policy, and the implementation of HPV vaccination in Indonesia.</p><p><strong>Methods: </strong>A scoping review was performed by collecting information from previous studies, including general information about vaccines, vaccine efficacy, effectiveness, and safety.</p><p><strong>Results: </strong>Approved HPV vaccine products in Indonesia have proven efficacy, effectiveness, and safety. Procuring vaccines through GAVI/UNICEF and the government has both advantages and disadvantages. Alongside the limited supply, numerous research studies show that dosage reduction to a single dose provides equal protection compared to 2-3 doses. The benchmark implementation of the single dose has been done in many countries, ranging from high-income to low-middle-income countries. Therefore, considering other countries and Indonesia's high population and vaccination burden, proposed updates for vaccination programs are recommended to achieve the cancer elimination target by 2030.</p><p><strong>Conclusion: </strong>Improvement of vaccination programs using single-dose HPV vaccine to prevent cervical cancer requires a coherent framework, sufficient funds, effective management of stakeholder interests, and sensitivity to contextual factors.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 2","pages":"421-434"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quality of Life of Indonesian Breast Cancer Women Undergoing Various Surgery Techniques.","authors":"Kristanto Yuli Yarso, Suyatmi Suyatmi, Ahmad Azmiardi, Monica Bellynda, Kamila Muyasarah, Danendra Rafi Noval Yarsa","doi":"10.31557/APJCP.2025.26.2.383","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.2.383","url":null,"abstract":"<p><strong>Introduction: </strong>Breast cancer has the highest cancer rate among women in the world. The types of surgery that are often performed are mastectomy, breast conserving surgery (BCS) without reconstruction, and mastectomy or BCS with reconstruction. In this study, we wanted to assess the QoL in patients with mastectomy without reconstruction, BCS without reconstruction, and BCS or mastectomy with reconstruction in breast cancer patientys in Indonesia.</p><p><strong>Method: </strong>This study was a retrospective cohort study that assessed patients' QoL after completing therapy with different  types of surgery. The inclusion criteria were all breast cancer patients who did a follow-up medical check-up at outpatient clinics from 2018-2023. Patients were disease-free, had been free of therapy, and were tumor-free for more than 6 months at the time of the evaluation.The participants' QoL was assessed using the SF-36 questionnaire. Data were recorded quantitatively and analyzed through bivariate and multivariate methods.</p><p><strong>Results: </strong>A significant difference in the total QoL score was found in the three groups (p=0.011). Physical function with a p-vcalue of 0.001  and vitality with a pvalue- of 0.015 were significantly different in the three group. After we conducted a post hoc test on these 3 groups, it was found that patients who underwent breast reconstruction surgery generally reported the best total QoL score and better physical functions score. On the other hand, patients who had BCS had the lowest vitality scores compared to those who underwent mastectomy and breast reconstruction groups.</p><p><strong>Conclusion: </strong>This research shows that the QoL of the breast reconstruction group has the highest value in the total QoL score and the physical function domain as well. Meanwhile, in the vitality domain, the BCS group had a significantly lower score than the mastectomy group and reconstruction group.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 2","pages":"383-389"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Community-based Assessment of Exposure to Tobacco Advertising, Promotion and Sponsorship (TAPS) among Adults Residing in an Urbanized Village in Delhi, India.","authors":"Yukti Bhandari, Jugal Kishore, Abhilasha Yadav, Aritrik Das","doi":"10.31557/APJCP.2025.26.2.557","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.2.557","url":null,"abstract":"<p><strong>Background: </strong>A comprehensive ban on tobacco advertising, promotion, and sponsorship (TAPS) is known to significantly reduce tobacco consumption and has been incorporated into the Framework Convention on Tobacco Control since 2003. Though Indian legislation also comprehensively bans TAPS, existing literature indicates poor and non-uniform implementation across the country. This study aims to assess the exposure to TAPS among adults residing in an urbanised village in South Delhi.</p><p><strong>Methods: </strong>It was a community-based cross-sectional house-to-house survey conducted in Aliganj, Delhi, between Apr 21 and Jun 21. 490 residents aged ≥ 15 years were included in the study. The interview was conducted using the Global Adult Tobacco Survey questionnaire. Data was analyzed using SPSS 21. Multivariable logistic regression analysis was conducted to identify the channels of communication where exposure to messaging encouraged thoughts of quitting.</p><p><strong>Results: </strong>Out of 490 study participants, 93(18.9%) were exposed to TAPS for smoked tobacco products, 88(17.9%) for smokeless tobacco products, and 74(15.1%) for both smoked and smokeless tobacco on various platforms. Exposure to TAPS for smokeless tobacco was more likely at stores where the products are sold (aOR = 2.19;95% C.I.-1.19-3.98), on television (aOR = 4.12;95% C.I.-1.49-11.39), billboards (aOR = 3.48;95% C.I.-1.18-10.29), and posters (aOR = 3.04;95% C.I.-1.22-7.60). Among smokers, 77.1%, and smokeless tobacco users, 75.4% had to quit thoughts triggered by packet warnings. Education and employment were found to be significantly associated.</p><p><strong>Conclusion: </strong>Almost one-fifth of the participants were exposed to TAPS through various channels. Our findings indicate that implementing the TAPS ban remains partial in our study area, especially regarding smokeless tobacco products, compared to state and national level estimates. Focusing on mass media anti-tobacco campaigns for both smoked and smokeless products, through television, newspapers, and magazines, in addition to comprehensive TAPS ban enforcement, can contribute effectively to reducing tobacco consumption in our study population.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 2","pages":"557-563"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Normal Saline Irrigation in Reducing Wound Contamination during Oral Cancer Surgery: A Cytological Analysis.","authors":"Pankhuri Shandilya, Ajay Vidyarthi, Hiralal Ash, Amit Kumar, Varun Ketan","doi":"10.31557/APJCP.2025.26.2.657","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.2.657","url":null,"abstract":"<p><strong>Background: </strong>Tumour manipulation during surgery can lead to the dissemination of malignant cells and potential wound contamination. Despite the widespread practice of irrigating surgical sites with normal saline, the efficacy of this measure in reducing epithelial contamination in oral cancer surgery remains unclear.</p><p><strong>Objectives: </strong>This study aimed to assess the proportion of normal saline wash effluents contaminated by epithelial cells or debris during oral cavity cancer surgery, and to evaluate the effectiveness of saline irrigation in reducing contamination.</p><p><strong>Methods: </strong>A total of 132 patients with biopsy-proven, treatment-naïve squamous cell carcinoma of the oral cavity undergoing surgery were included. Wash effluents from the tumour bed and neck incisions were collected post-irrigation with normal saline and analysed using cell block cytology. Cytology smears from the tumour bed and incision edges were examined for cellular contaminants. Data were analysed using Chi-square tests and Mann-Whitney tests.</p><p><strong>Results: </strong>Epithelial or abnormal epithelial cells were detected in 24% of cell block samples, while 21% showed cellular debris. Following normal saline irrigation, the positivity rate for epithelial cells or debris in smears decreased from 55% to 7.6%, a statistically significant reduction (p < 0.001). Perineural invasion was significantly associated with the presence of exfoliated cells (p = 0.037).</p><p><strong>Conclusions: </strong>Irrigation with normal saline significantly reduces the presence of exfoliated epithelial cells and cellular debris in wound sites during oral cancer surgery. The results support the continued use of mechanical cleansing measures during surgery to minimize the risk of tumour cell implantation.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 2","pages":"657-661"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liver Cancer Mortality Rates in Peru: Trend Analysis from 2003 to 2017.","authors":"J Smith Torres-Roman, Gabriel De La Cruz-Ku, Christian S Alvarez, Jorge Ybaseta-Medina, Eloy F Ruiz, Jose Fabian Martinez-Herrera, Janina Bazalar-Palacios, Lita Del Rio-Muñiz, Julio A Poterico, Katherine A McGlynn","doi":"10.31557/APJCP.2025.26.2.435","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.2.435","url":null,"abstract":"<p><strong>Background: </strong>Liver cancer is one of the leading causes of cancer-related death in Peru, and some reports have indicated an increase in mortality rates among the largest cities. To our knowledge, no study has been carried out at a national level or by geographic area in recent years. Thus, our objective was to examine overall, regional and sex-specific liver cancer mortality rates in Peru between 2003-2017.</p><p><strong>Methods: </strong>We retrieved data on liver cancer deaths between 2003 and 2017 from the mortality database of the Peruvian Ministry of Health.  Age-standardized mortality rates (ASMR) were estimated per 100,000 person-years using the world standard SEGI population. We analyzed mortality trends using Joinpoint regression Program Version 4.7.0. To examine the spatial distribution of the mortality rates, we used GeoDa software.</p><p><strong>Results: </strong>Between 2003 and 2017, 31,473 deaths from liver cancer were reported in Peru. Overall, liver cancer mortality rates have decreased significantly among Peruvian women since 2005 (-3.1% annually) with decreases in the coastal and highland regions ranging from 2.8% to 3.5%. In Arequipa, Cusco, La Libertad, Lima, and Moquegua, rates decreased bewteen 2003 and 2017. Among men in Ancash a significant increase (+12.5 annually) was observed from 2003-2011, followed by a sharpdecline (-10.5 annually) between  2011 and  2017.  In contrast, rates in Cajamarca, Junin, and Ucayali  decreased between  2003 and 2017.</p><p><strong>Conclusions: </strong>Although decreases in liver cancer mortality rates were observed in some  regions of Peru, these trends were not statistically significant. In addition, some provinces experienced  increases in rates. Effective interventions, such as expanding access to healthcare and controlling the various risk factors for liver cancer, remains a key challenge for the country.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 2","pages":"435-441"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Translation and Validation of the Greek Version of the Breast Cancer Screening Beliefs Questionnaire.","authors":"Aikaterini Toska, Dimitra Georga, Anastasios Christakis, Evangelos C Fradelos, Erasmia Rouka, Pavlos Sarafis, Maria Saridi","doi":"10.31557/APJCP.2025.26.2.663","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.2.663","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer is the most prevalent cancer among women in Greece. It is crucial to have a simple, trustworthy and effective instrument for assessing the factors that impact cancer screening behaviors.</p><p><strong>Objective: </strong>This study aimed to translate and validate the Greek version of the Breast Cancer Screening Beliefs Questionnaire (BCSBQ).</p><p><strong>Methods: </strong>In this cross-sectional methodological study conducted from April to June 2024, 202 Greek women aged 21 to 69 years participated using a snowball sampling method. A backward-forward translation process was performed on the BCSBQ. The study evaluated the face, content, and constructs validity of the questionnaire. A confirmatory factor analysis was carried out to determine whether the data supported the 3-factor structure of the instrument. Additionally, the reliability of the BCSBQ was measured using Cronbach's alpha and intra-class correlation coefficients.</p><p><strong>Result: </strong>The results of the confirmatory factor analysis demonstrated the presence of three distinct factors: attitude, knowledge, and barriers to screening. The three-factor model was supported by standard indices, with the following values: chi-square = 112.5, RMSEA = 0.079, SRMR = 0.057, CFI = 0.94, and NNFI = 0.942. The instrument also showed a satisfactory Cronbach's alpha of 0.814, while the Cronbach's alpha values for the three subscales ranged from 0.72 to 0.84. The mean scores observed on the three subscales in this study were remarkably high (Attitudes=76.7±23.9, Knowledge=80.9±20.1, Barriers=89.9±12.7).</p><p><strong>Conclusion: </strong>The three-factor structure of the BCSBQ-12 demonstrated valid and reliable results among Greek women. Given its strong psychometric properties, this tool can be utilized in future studies to assess women's beliefs regarding breast cancer screening in Greece.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 2","pages":"663-670"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plumbagin Inhibits Cadmium-Induced Interleukin-6/STAT3 Signaling in the Triple-Negative Breast Cancer Cell Line.","authors":"Titiwadee Titiwattanakarn, Rapeewan Settacomkul, Thitima Kasemsuk, Pornpun Vivithanaporn","doi":"10.31557/APJCP.2025.26.2.465","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.2.465","url":null,"abstract":"<p><strong>Introduction: </strong>Plumbagin has been found to reduce proinflammatory cytokine expression in activated macrophages and carrageenan-induced paw edema. Cadmium triggers the release of interleukin-6 (IL-6), a key mediator of inflammation and carcinogenesis in many cell types. The effects of plumbagin on cadmium-induced inflammation in triple-negative breast cancer cells are unknown.</p><p><strong>Method: </strong>We investigated the effects of plumbagin on cadmium-induced IL-6 expression and signal transducer and activator of transcription 3 (STAT3) activation in MDA-MB-231, a triple-negative breast cancer cell line, using real-time PCR, ELISA, and Western blotting.</p><p><strong>Result: </strong>Non-cytotoxic concentrations of cadmium chloride at 1 and 10 μM upregulated the IL-6 mRNA expression after 3 h of exposure and increased the IL-6 release after 24 h. Plumbagin at 4 μM or more was toxic to cells after 24 h. Plumbagin at 1 μM co-treated with cadmium reduced the expression and secretion of IL-6. At 24-h post-exposure, plumbagin decreased the levels of phosphorylated STAT3 induced by cadmium.</p><p><strong>Conclusion: </strong>Plumbagin inhibits cadmium-induced IL-6/STAT3 signaling in a triple-negative breast cancer cell and further in vivo studies are required to elucidate the potential use of plumbagin on cancer progression.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 2","pages":"465-470"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal Alcohol Consumption and Risk of Childhood Cancers: A Systematic Review and Meta-Analysis.","authors":"Roya Rashti, Faezeh Ghaaseemi, Jalal Poorolajal","doi":"10.31557/APJCP.2025.26.2.361","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.2.361","url":null,"abstract":"<p><strong>Background: </strong>Childhood cancers are a leading cause of death among children worldwide. Maternal alcohol consumption before and during pregnancy has been suggested as a potential risk factor for childhood cancers. However, the evidence to support this association is inconsistent. A systematic review and meta-analysis were conducted to clarify this association.</p><p><strong>Methods: </strong>We performed a systematic search of PubMed, Web of Science, and Scopus databases until May 2023 to identify observational studies reporting associations between maternal alcohol consumption and childhood cancers. Heterogeneity between studies was evaluated using the χ2, τ2, and I2 statistics. Publication bias was assessed using Begg and Egger tests. We calculated the odds ratio (OR) with a 95% confidence interval (CI) using a random-effects model.</p><p><strong>Results: </strong>Out of 18,583 studies retrieved from the search, 31 studies involving 47,277 participants met the eligibility criteria. Our meta-analysis found that maternal alcohol consumption before and during pregnancy was associated with an increased risk of childhood cancers with OR of 1.15 (95% CI: 1.00, 1.33) and 1.11 (95% CI: 1.03, 1.20), respectively.</p><p><strong>Conclusions: </strong>Our meta-analysis found evidence of a positive association between maternal alcohol consumption before and during pregnancy and the risk of childhood cancers. These findings suggest a need for public health interventions aimed at reducing alcohol consumption during pregnancy to potentially prevent childhood cancers.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 2","pages":"361-369"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Targeting of PARP Expression and Glycolysis Rate-Limiting Enzymes in Breast Cancer Patients.","authors":"Shams Firas Adnan, Zainab N Najim Al-Abady","doi":"10.31557/APJCP.2025.26.2.611","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.2.611","url":null,"abstract":"<p><strong>Background: </strong>Breast cancer is a heterogeneous disease characterized by diverse biochemical, histological, and clinical features. PARP1 and glycolysis rate-limiting enzymes play critical roles in cancer progression, making them promising therapeutic targets.</p><p><strong>Aim: </strong>This study aimed to evaluate the expression levels of PARP1 and key glycolytic enzymes (HK, PFK, and PK) in breast cancer patients and assess their potential as therapeutic indicators.</p><p><strong>Materials and methods: </strong>A total of 120 participants (60 breast cancer patients and 60 healthy controls) were included in the study. Blood samples were collected to measure PARP1 expression and the levels of glycolytic enzymes using ELISA. Statistical analyses were performed to compare the two groups.</p><p><strong>Results: </strong>PARP1 expression and glycolytic enzyme levels (HK, PFK, and PK) were significantly higher in breast cancer patients compared to healthy controls (p < 0.0001).</p><p><strong>Conclusion: </strong>The overexpression of PARP1 and key glycolytic enzymes indicates their involvement in breast cancer progression and underscores their potential as therapeutic targets and biomarkers.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 2","pages":"611-617"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High APEX1 Expression Facilitates Osteosarcoma Cell Proliferation.","authors":"Yan Yu Lu, Wen Lu, Jie Zheng, Ju Shu Luo","doi":"10.31557/APJCP.2025.26.2.453","DOIUrl":"https://doi.org/10.31557/APJCP.2025.26.2.453","url":null,"abstract":"<p><p>Osteosarcoma (OS) is a serious malignancy affecting children and young adults; however, there is limited improvement in the survival of patients with OS over the past four decades. Molecular targeted therapy is a promising treatment strategy for OS. Apurinic/apyrimidinic exonuclease 1 (APEX1)-a key factor for DNA damage repair-is associated with OS proliferation, but the underlying molecular mechanism remains unclear. APEX1 expression in OS tissues and paired paracancerous tissues and in human osteoblast cell line hFOB1.19 and OS cell lines was determined using real-time quantitative PCR (RT-qPCR). APEX1-shRNA and NC-shRNA lentiviral vectors were constructed and transfected into MG-63 cells. The effects of APEX1 knockdown on MG-63 cell proliferation and apoptosis were assessed using MTT, xenograft tumor growth, and terminal deoxynucleotidyl transferase dUTP nick end labeling assays. Expression changes of apoptosis- and angiogenesis-related genes due to APEX1 knockdown were detected using RT-qPCR and immunohistochemistry. To preliminarily determine the mechanism by which APEX1 affects OS cell proliferation, transcription factors were predicted using three databases, and construction of protein-protein interaction network, gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses were performed. APEX1 expression was higher in OS tissues than in paracancerous tissues. APEX1 expression was also higher in OS cell lines than in hFOB1.19 cells, with the highest APEX1 expression observed in MG-63 cells. APEX1 knockdown mediated by APEX1-shRNA lentivirus markedly suppressed MG-63 cell proliferation both in vitro and in vivo and induced their apoptosis. APEX1 knockdown downregulated CD31 expression but had no effect on the expression of P53 and Caspase3. Bioinformatics analyses suggested that USF1 or SP1 regulates APEX1 transcription and its recruitment in DNA damage response pathways, affecting OS cell proliferation. Thus, high APEX1 expression in OS facilitates cell proliferation likely via CD31, and USF1 or SP1 may regulate APEX1 transcription and its recruitment in DNA damage response pathways.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"26 2","pages":"453-463"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143532244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}