Asian Pacific Journal of Cancer Prevention最新文献

{"title":"CD44 Expression in Head and Neck Cancer: Systematic Review, IHC-based Meta-Analysis, and Pathway Analysis Highlight a Favorable Clinicopathological Association.","authors":"Stella Marleen, Muhammad Husni Cangara, Upik Anderiani Miskad, Prihantono Prihantono","doi":"10.31557/APJCP.2026.27.9.3497","DOIUrl":"10.31557/APJCP.2026.27.9.3497","url":null,"abstract":"<p><strong>Background: </strong>Head and neck cancer (HNC) is the seventh most common cancer worldwide with its highest incidence in Asia. Tobacco, alcohol, and HPV contribute to this phenomenon. CD44 has been found to have a mechanistic role in tumorigenicity and metastasis, however current literature shows conflicting evidence regarding its role in HNC. This review attempts to synthesize available studies to clarify its clinicopathological significance.</p><p><strong>Methods: </strong>A PRISMA-compliant systematic search was performed using PubMed, Cochrane, and the Directory of Open Access Journals for studies comparing clinicopathological features between immunohistochemically assessed CD44 expression levels. Meta-analysis was conducted utilizing Review Manager 5.4. Further subgroup analysis was conducted based on the CD44 expression quantification method.</p><p><strong>Results: </strong>Nine studies, representing 484 patients, were included in the final analysis. An association between CD44 expression and histopathological grading (OR = 0.46, 95% CI: 0.25-0.84) was found. Gender, survival, lymph node involvement, and perineural invasion were not found to be predicted by CD44.</p><p><strong>Conclusions: </strong>This meta-analysis demonstrates that CD44 expression is associated with histopathological grading in HNC. However, survival, lymph node involvement, and perineural invasion are found to be independent of CD44 expression.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"27 9","pages":"3497-3507"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149118950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-126 and miR-143/145 Polymorphisms in Prostate Cancer: Clinical and Methodological Perspectives.","authors":"Akif Erbin, Serbay Arslan","doi":"10.31557/APJCP.2026.27.9.3133","DOIUrl":"https://doi.org/10.31557/APJCP.2026.27.9.3133","url":null,"abstract":"","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"27 9","pages":"3133"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KRAS and BRAF Hotspot Variants Show Lower Prevalence in Bangladeshi Colorectal Cancer Patients.","authors":"Muhammad Sibgatullah Zunnun, Md Zahin Alam, S M Mahbubur Rashid, Md Shihab Al Mashiur Rahaman, Nahid Parvez, Md Shahadot Hossain Sheikh, Farida Arjuman, Mustak Ibn Ayub","doi":"10.31557/APJCP.2026.27.9.3221","DOIUrl":"https://doi.org/10.31557/APJCP.2026.27.9.3221","url":null,"abstract":"<p><strong>Objective: </strong>Colorectal cancer (CRC) is the second-leading cause of cancer-related mortality globally, with a rising incidence in Bangladesh. This study aims to identify pathogenic KRAS and BRAF mutations contributing to anti-EGFR resistance in CRC patients.</p><p><strong>Methods: </strong>Two sets of primers were used: one was adopted from a published paper, and another was designed using Primer3Plus. They were optimized for specific PCR amplicons of 173 bp and 230 bp from exon 2 of KRAS and exon 15 of BRAF, respectively. Targeted Sanger sequencing was performed to analyze mutations in these amplicons in 46 CRC patient samples.</p><p><strong>Results: </strong>KRAS mutations were detected in 5 samples (10.87%), comprising two G12D, one G12V, and two G13D variants. Notably, no BRAF V600E mutation was identified. These results stand in sharp contrast to global data, where KRAS mutations at these loci occur in approximately 40% of cases and BRAF V600E mutations in about 10%. Such mutations are clinically significant because they confer resistance to anti-EGFR therapy, underscoring the distinct molecular profile observed in this cohort.</p><p><strong>Conclusion: </strong>The lower prevalence of KRAS and BRAF mutations indicates the potential effectiveness of anti-EGFR therapies in Bangladeshi CRC patients than other populations.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"27 9","pages":"3221-3226"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Diabetes, Hypertension, and Gut Microbial Diversity on Chemotherapy Response and Survival in Cancer Patients: A Prospective Cohort Study.","authors":"Sobia Nadeem, Rajib Maitra, - Farah, Shovit Dutta, Khaizran Siddiqui, Rabia Zulfiqar, Mamtaz Mariam Asha, Salah Termos","doi":"10.31557/APJCP.2026.27.9.3377","DOIUrl":"https://doi.org/10.31557/APJCP.2026.27.9.3377","url":null,"abstract":"<p><strong>Background and aims: </strong>Chronic comorbidities, including diabetes mellitus and hypertension, and gut microbial factors may influence chemotherapy response and survival in cancer patients. This study quantitatively evaluated their combined impact. The main objective of this study was to evaluate the impact of diabetes, hypertension, and gut microbial diversity on chemotherapy response and survival in cancer patients.</p><p><strong>Methods: </strong>A prospective cohort study enrolled 250 adult cancer patients receiving chemotherapy at a tertiary care center in Bangladesh. Clinical, metabolic, and microbiome data were collected at baseline and during treatment. Chemotherapy response was assessed by RECIST 1.1 criteria, while progression-free survival (PFS) and overall survival (OS) were recorded. Gut microbial diversity was measured using 16S rRNA sequencing. Statistical analyses included chi-square tests, t-tests, Kaplan-Meier survival analysis, and multivariable Cox regression.</p><p><strong>Results: </strong>Among participants, 32.8% had diabetes and 38.4% had hypertension. Objective response rates (CR+PR) were lower in patients with diabetes (41.5%) and hypertension (45.8%) compared with non-diabetic (57.1%) and normotensive (55.8%) patients (p < 0.05). Responders exhibited significantly lower HbA1c, fasting glucose, and systolic blood pressure than non-responders (p < 0.01). Higher gut microbial alpha diversity was observed in responders (Shannon index 4.1 ± 0.6) versus non-responders (3.4 ± 0.7; p < 0.001). Median PFS and OS were shorter in patients with diabetes (8.6 and 18.4 months) and hypertension (9.1 and 20.2 months) compared with those without comorbidities (12.9 and 26.7 months; 13.4 and 27.9 months, respectively). Multivariable analysis confirmed diabetes (aHR 1.58), hypertension (aHR 1.41), low microbial diversity (aHR 1.76), and advanced tumor stage (aHR 2.34) as independent predictors of worse survival (all p < 0.05).</p><p><strong>Conclusions: </strong>Diabetes, hypertension, and reduced gut microbial diversity are associated with poorer chemotherapy response and survival outcomes. Integrated management of metabolic and cardiovascular comorbidities, alongside microbiome-informed interventions, may enhance treatment efficacy and patient survival.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"27 9","pages":"3377-3383"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Maintenance Capecitabine Therapy After First-Line Chemotherapy in Metastatic Pancreatic Cancer.","authors":"Mohamed Abouelfetouh Shehata, Alshimaa Mahmoud Alhanafy, Mohamed Elsherbini Hassan, Nahla Atef Shaaban, Amira Hosny Hegazy","doi":"10.31557/APJCP.2026.27.9.3203","DOIUrl":"10.31557/APJCP.2026.27.9.3203","url":null,"abstract":"<p><strong>Objective: </strong>To assess the clinical impact of maintenance capecitabine following first-line modified FOLFIRINOX in patients with metastatic pancreatic cancer (mPC), focusing on progression-free survival (PFS), overall survival (OS), quality of life (QoL), and the toxicity profile.</p><p><strong>Methods: </strong>This phase II prospective trial, 60 patients with histologically confirmed stage IV pancreatic adenocarcinoma (ECOG PS ≤1, normal organ function, and measurable metastatic disease) who were enrolled after completing modified FOLFIRINOX. Participants were randomized into two groups: Arm A received maintenance capecitabine, and Arm B underwent observation only.</p><p><strong>Results: </strong>Baseline patient and disease characteristics were comparable between both groups. The pancreatic head was the predominant tumor site (73.3% in Arm A vs. 86.7% in Arm B). After six months of maintenance therapy, stable disease was reported in 33.3% of Arm A and 0% of Arm B. Toxicity levels were not significantly different. Patients in Arm B demonstrated earlier deterioration in QoL, with median time to decline of 3.2 months compared with 6.9 months in Arm A. Median PFS and OS favored the capecitabine arm (6.13 vs. 3.57 months and 14.20 vs. 10.97 months, respectively). Multivariate Cox regression confirmed treatment arm as the most independent prognostic factor influencing both PFS and OS.</p><p><strong>Conclusion: </strong>Maintenance capecitabine after first-line modified FOLFIRINOX in mPC was well tolerated and may enhance PFS and OS while maintaining an acceptable quality of life.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"27 9","pages":"3203-3212"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methodological Considerations in Assessing Lung Cancer Knowledge, Attitude and Screening Practice in Uzbekistan: A Cross-Sectional Study.","authors":"Vaishnavi Gawande, Sana Anwar","doi":"10.31557/APJCP.2026.27.9.3131","DOIUrl":"https://doi.org/10.31557/APJCP.2026.27.9.3131","url":null,"abstract":"","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"27 9","pages":"3131-3132"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimizing Antimicrobial Treatment to Cryopreserve and Establish Oral Squamous Cell Carcinoma In-Vitro Models Derived from Habitual Tobacco Chewers.","authors":"Sowmya Sv, Varsha Vinay Kumar, Aditya Sreejit, Dominic Augustine, Aastha Lal, Kavitha Prasad, Kavina Ganapathy","doi":"10.31557/APJCP.2026.27.9.3399","DOIUrl":"https://doi.org/10.31557/APJCP.2026.27.9.3399","url":null,"abstract":"<p><strong>Objectives: </strong>Cryopreservation and establishment of reliable in vitro models of oral squamous cell carcinoma (OSCC) are frequently compromised by microbial contamination arising from the oral microbiota of habitual tobacco users. This study aimed to optimize an antimicrobial strategy prior to biobanking to eliminate resistant microbial contaminants and improve the reliability of OSCC primary cultures.</p><p><strong>Methods: </strong>OSCC tissues obtained from habitual tobacco chewers were subjected to either conventional penicillin-streptomycin (Pen-Strep) treatment or a stepwise antimicrobial treatment before cryopreservation. The optimized protocol employed a combination of cell culture-grade antibiotics to selectively eliminate Pen-Strep-resistant microbial taxa. Post-thaw tissue viability and epithelial outgrowth were assessed. Microbial profiling of conventionally treated tissues was performed using 16S and 18S rRNA gene sequencing, and antibiotic susceptibility testing was carried out to determine resistance profiles.</p><p><strong>Results: </strong>Conventional Pen-Strep treatment alone was insufficient to eliminate microbial contamination, whereas the stepwise antimicrobial treatment effectively eradicated resistant microorganisms, enabling sterile tissue preservation. Post-thaw assessments demonstrated epithelial-like outgrowth from day 3 onward, confirming preserved tissue viability and integrity. Microbial profiling identified contaminants including Neisseria elongata, Streptococcus gallolyticus, and Candida albicans. Antibiotic susceptibility testing highlighted the necessity of tailored combinatorial antimicrobial strategies to overcome resistance.</p><p><strong>Conclusion: </strong>The refined biobanking protocol integrating a stepwise antimicrobial strategy prior to cryopreservation effectively mitigates microbial contamination while preserving OSCC tissue integrity. This approach enhances the reliability of OSCC primary cultures derived from tobacco chewers and provides a robust platform for translational oncology research and personalized medicine.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"27 9","pages":"3399-3410"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Knowledge, Attitudes, and Perceived Impact of the Single-Stick Cigarette Ban: A Cross-Sectional Study in Indonesia.","authors":"Abdillah Ahsan, Fitri Kurnia Rahim, Indah Suci Widyahening, Depi Yulyanti, Witri Dewi Mentari, Yudita Ingga Hindiarti, Humaira Anggie Nauli, Ernoiz Antriyandarti, Made Adhyatma Prawira Natha Kusuma, Maulida Gadis Utami, Yuyu Buono Ayuning Pertiwi, Ella Kurnia, Anisa Salsabila","doi":"10.31557/APJCP.2026.27.9.3185","DOIUrl":"https://doi.org/10.31557/APJCP.2026.27.9.3185","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate public perceptions (knowledge, attitudes, and perceived impact) of the single-stick cigarette sales ban in Indonesia, and to identify sociodemographic and behavioral factors associated with these outcomes.</p><p><strong>Methods: </strong>A cross-sectional survey study was conducted among 714 respondents in seven selected districts in Indonesia, utilizing the Knowledge, Attitude, and Perception (KAP) framework. Multivariable analyses were performed using binary logistic regression for knowledge and ordered logistic regression for attitude and perception. Robust standard errors were applied, and findings are reported as adjusted odds ratios (aORs) with 95% confidence intervals (95% CIs).</p><p><strong>Result: </strong>Most respondents (66.11%) were unaware of the regulation. Older adults (≥55) had lower knowledge (aOR = 0.47, 95% CI: 0.28-0.79), and active smokers opposed the ban (aOR = 0.35, 95% CI: 0.23-0.51). Higher education (aOR = 2.16, 95% CI: 1.00-4.63) and urban residence (aOR = 1.65, 95% CI: 1.08-2.51) correlated with supportive attitudes. Knowledge (aOR = 1.90, 95% CI: 1.25-2.87) and supportive attitudes (aOR = 2.86, 95% CI: 1.56-5.22) were the strongest predictors of perceiving the ban as effective for public health.</p><p><strong>Conclusion: </strong>The implementation of the single-stick cigarette ban in Indonesia has been ineffective, primarily due to a lack of public awareness of the regulation. Smokers and older adults demonstrate significantly limited knowledge of and acceptance toward the policy. These findings highlight the need for increased public awareness campaigns and targeted interventions to improve knowledge and compliance with the single-stick cigarette sales ban in Indonesia.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"27 9","pages":"3185-3191"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global Trends and Disparities in Cervical Cancer Control in Central Asia (1990-2023): A Comparative Age-Period-Cohort and Joinpoint Regression Analysis.","authors":"Dinara Serikova-Esengeldina, Balday Issenova, Zukhra Siyazbekova, Umutzhan Samarova, Saule Rakhyzhanova, Kamila Akhmetova, Assiya Yessenbayeva, Zaituna Khismetova, Aliya Atabayeva","doi":"10.31557/APJCP.2026.27.9.3307","DOIUrl":"https://doi.org/10.31557/APJCP.2026.27.9.3307","url":null,"abstract":"<p><strong>Background: </strong>Cervical cancer is a significant public health challenge that remains a leading cause of premature death among women in Central Asia, serving as a marker of health inequality. Following the dissolution of the Soviet Union, healthcare strategies diverged, creating disparate outcomes for women across the region. This study evaluates the long-term trends in cervical cancer incidence, mortality, and disability-adjusted life years (DALYs) across Kazakhstan, Kyrgyzstan, Tajikistan, Turkmenistan, and Uzbekistan, distinguishing between the life-saving potential of organized screening and the limitations of opportunistic approaches.</p><p><strong>Methods: </strong>We conducted a comparative ecological study using the Global Burden of Disease (GBD) Study 2023 dataset (1990-2023). We analyzed trends in Age-Standardized Rates (ASR) and the absolute human burden of disease. Joinpoint regression was used to calculate the Average Annual Percent Change (AAPC). An Age-Period-Cohort (APC) model (intrinsic estimator) was employed to isolate \"period effects\" attributable to screening interventions from demographic and cohort factors. Ovarian cancer served as a descriptive comparative reference to contrast trends with another gynecological malignancy that lacks an organized population-based screening programme in the region.</p><p><strong>Results: </strong>Kazakhstan demonstrated a robust decline in mortality (ASR AAPC: -2.5%; 95% CI: -2.8 to -2.2), associated with a strong period effect following the scale-up of its organized national screening programme. In contrast, neighboring countries exhibited slower declines and face a critical demographic divergence (the 'scissors effect'): while statistical rates have stabilized, the absolute number of lives lost has nearly doubled since 1990 due to population growth. Furthermore, the disease affects women in the region at a younger age than the global average, amplifying the socioeconomic loss.</p><p><strong>Conclusion: </strong>A widening gap in cancer control exists within Central Asia. Kazakhstan's experience indicates organized screening reduces mortality. To ensure regional health equity, neighboring countries must urgently transition to organized national programmes. This may be accelerated by adopting, technology-driven solutions like Artificial Intelligence (AI) to bridge resource gaps in underserved communities.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"27 9","pages":"3307-3313"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The First Molecular Profiling of JAK2 Exon 12 and Flanking Intronic Sequences in Indonesia.","authors":"Farmaditya Eka Putra Mundhofir, Nani Maharani, Raisa Tahira, Amelia Syabani, Nydia Rena Benita Sihombing, Ferdy Kurniawan Cayami, Endang Mahati, Tri Indah Winarni","doi":"10.31557/APJCP.2026.27.9.3237","DOIUrl":"https://doi.org/10.31557/APJCP.2026.27.9.3237","url":null,"abstract":"<p><strong>Objective: </strong>Data on JAK2 Exon 12 mutations in Indonesia are not available. This study aimed to explore the presence of known JAK2 exon 12 coding mutations in V617F-negative myeloproliferative neoplasm (MPN) patients in Indonesia and to describe any novel intronic changes downstream of exon 12.</p><p><strong>Methods: </strong>A total of 198 V617F-negative MPN samples were screened using allele-specific polymerase chain reaction (AS-PCR) for four commonly reported canonical exon 12 mutations. Of these, 57 cases were selected at random or for atypical PCR band patterns and underwent bidirectional Sanger sequencing of a 496-bp fragment covering exon 12 plus ~250 bp of the adjacent region. Variants were cross-referenced with gnomAD v4.1.0. Furthermore, SpliceAI was used to predict splice site gain or loss.</p><p><strong>Results: </strong>No coding mutations were identified in exon 12. However, two intronic variants were detected adjacently downstream: NC_000009.12:g.5070231_5070235delTCTTA in 29 of 57 cases (50.9%), which was not present in the gnomAD database, and NC_000009.12:g.5070319C>T in 1 of 57 cases (1.8%), corresponding to a rare gnomAD entry. The SpliceAI Δ scores remained below 0.2, a high-sensitivity threshold, indicating no predicted impact on canonical splicing.</p><p><strong>Conclusion: </strong>Classic exon 12 coding mutations are apparently absent in Indonesian V617F-negative MPNs. We report a highly frequent downstream intronic deletion not recorded in global databases, as well as a rare single nucleotide variant (SNV) that aligns with gnomAD data. Although in silico tools suggest minimal splice disruption, RNA-level assays are needed to rule out the subtle effects of the variant. Our workflow, which combines allele-specific PCR and targeted sequencing, may be suitable for laboratories facing resource constraints.</p>","PeriodicalId":55451,"journal":{"name":"Asian Pacific Journal of Cancer Prevention","volume":"27 9","pages":"3237-3243"},"PeriodicalIF":0.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149076882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}