Effects of PIK3CA Gene Modifications on Radiosensitivity in Colorectal Cancer Cells.

The phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) plays a critical role in cell growth and survival. PIK3CA somatic mutations are linked to the PIK3CA-related overgrowth spectrum (PROS) syndrome. Cells from those patients appeared to be associated with a moderate but significant radiosensitivity. Mutations or amplifications in this gene are common in breast, colorectal, and lung cancers. Alterations in the PIK3CA gene, including amplification and mutation, are common in cancer, but their influence on radiotherapy is not yet fully understood. This report reveals a potential association between PIK3CA gene modifications and radiosensitivity (p < 0.05) deducted from 8 established colorectal cancer cell lines (HT-29, DLD-1, HCT-116, SW480, HCT-15, Colo-320, and LoVo). Meanwhile gene amplification (> 2) seems to be linked to increased radiation sensitivity, mutations appear to be associated with increased radioresistance in colorectal cancer cells. Leveraging this relationship, PIK3CA amplification and mutations could act as biomarker to pinpoint patients who might benefit from more personalized radiotherapy regimens. However, this association is preliminary and hypothesis-generating in view of the limited number of cases. Further studies are needed to confirm this conclusion. By uncovering the distinct mechanistic effects of these PIK3CA alterations on radiosensitivity phenotype in both normal and cancerous cells, researchers can lay the groundwork for tailored radiotherapy strategies in colorectal cancer. This insight could enhance treatment effectiveness while reducing side effects, ultimately leading to improved patient outcomes.